##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665009
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	845214
Total Bases	210.4 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.100217223093797	28.0	18.0	32.0	18.0	33.0
2	26.643398003345897	28.0	25.0	31.0	18.0	33.0
3	25.662035886769505	27.0	18.0	31.0	18.0	33.0
4	29.979950639719647	31.0	29.0	33.0	27.0	33.0
5	31.65884261264011	33.0	32.0	33.0	29.0	33.0
6	35.71677705291204	37.0	36.0	38.0	31.0	38.0
7	35.90097537428391	38.0	36.0	38.0	31.0	38.0
8	36.45852529655212	38.0	37.0	38.0	34.0	38.0
9	36.697441121420134	38.0	38.0	38.0	34.0	38.0
10-14	36.732230654011886	38.0	38.0	38.0	34.0	38.0
15-19	36.689033073280854	38.0	38.0	38.0	34.0	38.0
20-24	36.68660812527951	38.0	38.0	38.0	34.0	38.0
25-29	36.61922187753634	38.0	38.0	38.0	34.0	38.0
30-34	36.59166152004108	38.0	38.0	38.0	34.0	38.0
35-39	36.52522887694714	38.0	38.0	38.0	34.0	38.0
40-44	36.54179817182394	38.0	38.0	38.0	34.0	38.0
45-49	36.48085620919672	38.0	38.0	38.0	34.0	38.0
50-54	36.35861261171727	38.0	37.2	38.0	33.6	38.0
55-59	36.228787265710224	38.0	37.0	38.0	33.0	38.0
60-64	36.2083406095971	38.0	37.0	38.0	33.0	38.0
65-69	36.14217346139558	38.0	37.0	38.0	33.0	38.0
70-74	36.0594642303606	38.0	37.0	38.0	32.6	38.0
75-79	35.980700878745225	38.0	37.0	38.0	31.8	38.0
80-84	35.90433989985913	38.0	37.0	38.0	31.0	38.0
85-89	35.784919122777495	38.0	36.6	38.0	31.0	38.0
90-94	35.69435769642142	38.0	36.0	38.0	31.0	38.0
95-99	35.58726675271705	38.0	36.0	38.0	29.6	38.0
100-104	35.472144308543506	38.0	36.0	38.0	29.0	38.0
105-109	35.30620134529586	38.0	35.2	38.0	29.0	38.0
110-114	35.19494665937673	38.0	35.0	38.0	28.0	38.0
115-119	35.062992082899925	38.0	35.0	38.0	28.0	38.0
120-124	35.178249978318505	38.0	35.0	38.0	28.8	38.0
125-129	33.23975589123544	34.4	32.8	35.6	29.2	35.6
130-134	34.5409194417675	36.0	35.4	36.6	30.2	37.2
135-139	35.84578815243126	38.0	37.0	38.0	30.8	38.0
140-144	35.758709830935985	38.0	37.0	38.0	29.4	38.0
145-149	35.73364171752864	38.0	37.0	38.0	29.0	38.0
150-154	35.705848369825375	38.0	37.0	38.0	29.0	38.0
155-159	35.697158936987265	38.0	37.0	38.0	29.0	38.0
160-164	35.65862817582498	38.0	37.0	38.0	29.0	38.0
165-169	35.625568342304724	38.0	37.0	38.0	29.0	38.0
170-174	35.57647522705846	38.0	37.0	38.0	29.0	38.0
175-179	35.53589593273884	38.0	36.8	38.0	29.0	38.0
180-184	35.4294813279005	38.0	36.0	38.0	28.8	38.0
185-189	35.35980692245601	38.0	36.0	38.0	28.0	38.0
190-194	35.30107255262574	38.0	36.0	38.0	28.0	38.0
195-199	35.218362968245295	38.0	36.0	38.0	28.0	38.0
200-204	35.10050240992168	38.0	36.0	38.0	27.0	38.0
205-209	34.98863313406239	38.0	35.8	38.0	26.6	38.0
210-214	34.867121877714204	38.0	35.0	38.0	25.8	38.0
215-219	34.684932110319224	38.0	35.0	38.0	25.0	38.0
220-224	34.53192584097927	38.0	35.0	38.0	23.8	38.0
225-229	34.306202443331664	38.0	34.0	38.0	23.0	38.0
230-234	34.15853836005034	38.0	34.0	38.0	23.0	38.0
235-239	34.032288548906	38.0	34.0	38.0	23.0	38.0
240-244	34.03648482242587	38.0	34.0	38.0	23.0	38.0
245-249	34.36014468830986	38.0	33.8	38.0	24.4	38.0
250-252	35.67106840256792	37.666666666666664	34.666666666666664	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	2.0
17	10.0
18	12.0
19	24.0
20	90.0
21	352.0
22	1086.0
23	2121.0
24	3699.0
25	5615.0
26	7985.0
27	10714.0
28	13742.0
29	17117.0
30	21259.0
31	26397.0
32	32839.0
33	42460.0
34	59446.0
35	91634.0
36	179467.0
37	329141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75152183847085	20.064235671278155	14.159312988485881	21.02492950176512
2	16.70878617722849	20.83791797107005	37.55545932746027	24.8978365242412
3	16.976883960748403	28.8929194263228	31.565733648519785	22.564462964409014
4	12.663538464814827	21.38760124654821	42.64754251585989	23.30131777277707
5	13.051724178728701	38.1996748752387	36.335886532878064	12.412714413154537
6	23.807935031838092	42.474805197263656	21.818143097487734	11.89911667341052
7	23.239913205413067	32.54181781182044	28.707167652215887	15.511101330550606
8	22.543048269432354	36.24277401936078	22.460110693859782	18.754067017347086
9	23.076759258601964	17.49911856642223	24.119690397934722	35.30443177704108
10-14	22.243928756504268	25.021095249250486	30.282697636338252	22.45227835790699
15-19	22.046747924194346	29.177249785261484	27.322713537636623	21.453288752907547
20-24	21.975343405780812	28.148483808757586	27.91621017261982	21.959962612841778
25-29	22.158821474916873	27.9701175666607	27.875844646557002	21.99521631186542
30-34	22.107087672471117	27.98034580591424	27.90829304767787	22.004273473936777
35-39	22.084963098102968	27.807774125842688	28.035811049036102	22.071451727018246
40-44	21.96560870974688	27.71785606958711	28.03067625477098	22.285858965895027
45-49	21.67998568871633	27.529722889251296	28.44862182906156	22.34166959297081
50-54	21.485166601830002	27.59732361824551	28.372997280646207	22.544512499278277
55-59	21.32262968900668	27.364690988470112	28.533178733901643	22.77950058862156
60-64	21.241715848424665	27.355855703431452	28.192535194703446	23.209893253440438
65-69	21.13030276068219	27.431688118446385	27.948594291599065	23.489414829272356
70-74	21.216256126859356	27.191115414979354	27.83196265857893	23.760665799582362
75-79	21.099103427139994	27.18159948976875	27.756840258097494	23.962456824993758
80-84	21.04271764065008	27.070273805788293	27.66861610064929	24.218392452912344
85-89	21.01249419943439	26.80047071841351	27.843519493331687	24.343515588820413
90-94	21.088034530862313	26.57373718729183	27.721784236538298	24.61644404530756
95-99	21.11021081198252	26.23854761136232	27.83658119399227	24.81466038266289
100-104	20.980243481191287	26.167010054568145	27.99771011624071	24.855036347999853
105-109	20.84168749290015	26.437578098375553	28.0101243894127	24.7106100193116
110-114	20.65177438964788	27.010328407819568	27.706730872777563	24.63116632975499
115-119	20.51843291792674	27.290148974486677	27.650442075682147	24.54097603190444
120-124	20.43313818721999	27.55066766867874	27.618052507137442	24.398141636963828
125-129	21.048795586570073	26.749111527507296	29.403276802996153	22.798816082926475
130-134	19.6146515149604	33.24918803796129	29.69933070100639	17.436829746071915
135-139	22.45888391165871	24.585388582492037	29.707599361103863	23.248128144745383
140-144	21.689906166296396	28.20477126012058	27.701907133379404	22.40341544020362
145-149	21.80607609515583	28.21315115042226	28.114587720738538	21.866185033683376
150-154	21.924054319867746	27.99376435674523	28.0346943918475	22.04748693153952
155-159	21.869538564634837	27.989368405822486	28.0083822919086	22.132710737634074
160-164	21.80374438284269	27.793681273635613	28.258593777702046	22.14398056581965
165-169	21.815287192612345	27.92457185556424	27.836830951811514	22.4233100000119
170-174	21.607343515526725	27.74212217394636	28.14526449953572	22.50526981099119
175-179	21.71931002757919	27.53257255155243	28.09869681236511	22.649420608503263
180-184	21.710362867415036	27.376971469203244	28.2406865362777	22.67197912710402
185-189	21.51366698279237	27.157736413040247	28.410712734550792	22.917883869616592
190-194	21.422476650539846	27.265881214969262	28.3743929830591	22.93724915143179
195-199	21.35863441931946	27.156167806245495	28.47157734166726	23.013620432767784
200-204	21.506649736715765	26.9199316832664	28.29229122055675	23.281127359461088
205-209	21.59919123860892	26.59606210227438	28.361605187359718	23.44314147175698
210-214	21.629216633116442	26.649033016330055	28.297403935695236	23.424346414858267
215-219	21.65395134315215	26.731316505667518	27.95225527085575	23.662476880324576
220-224	21.89860383241387	26.26531882132085	28.421890550125696	23.414186796139592
225-229	21.7532050842148	26.59451178915731	28.07726731280028	23.57501581382761
230-234	21.800728189091338	26.642799783431332	28.09764907479021	23.45882295268712
235-239	21.955967641647696	26.48437167702523	28.31569726260023	23.243963418726846
240-244	21.785609321088803	26.409164475829666	28.10573791282012	23.69948829026141
245-249	21.567186055065644	26.89432019351901	28.148224808746868	23.39026894266847
250-252	20.947251423435347	26.606779073702146	28.52912827524608	23.916841227616427
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.3333333333333333
13	0.6666666666666666
14	0.0
15	1.3333333333333333
16	1.6666666666666665
17	7.666666666666663
18	14.833333333333341
19	31.499999999999957
20	53.0000000000001
21	86.83333333333319
22	165.83333333333363
23	279.6666666666683
24	480.4999999999904
25	768.8333333333495
26	1218.500000000004
27	1769.333333333213
28	2518.3333333333544
29	3474.0000000004566
30	4616.000000000288
31	6047.666666665662
32	7587.833333330941
33	9101.833333331917
34	10663.33333333475
35	12201.833333337509
36	13705.8333333402
37	14815.166666675532
38	15969.16666667759
39	16961.000000010386
40	18059.166666673125
41	18712.333333337494
42	19463.16666666815
43	20181.999999998923
44	20443.999999997995
45	19900.50000000004
46	18932.000000003525
47	18285.00000000578
48	17769.66666667434
49	17223.500000009608
50	16307.500000011385
51	15431.333333343086
52	14194.666666674213
53	12843.00000000516
54	11206.333333335553
55	9425.499999999049
56	7856.833333330755
57	6473.833333332005
58	5084.833333333259
59	3950.5000000006435
60	2949.0000000001946
61	2254.8333333332166
62	1650.1666666665799
63	1146.1666666666936
64	862.5000000000248
65	661.333333333337
66	495.33333333332337
67	366.49999999999744
68	248.50000000000247
69	178.33333333333388
70	130.66666666666595
71	94.99999999999977
72	77.00000000000001
73	55.66666666666677
74	41.66666666666668
75	26.83333333333331
76	22.333333333333325
77	16.833333333333343
78	12.333333333333336
79	9.499999999999998
80	4.666666666666667
81	3.3333333333333335
82	2.6666666666666665
83	1.9999999999999998
84	0.6666666666666666
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060576374740598245
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	4.732529276609238E-4
25-29	9.938311480879398E-4
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.045299770235703E-4
50-54	0.0015617346612810485
55-59	0.002247951406389388
60-64	0.0018693490642606489
65-69	0.0037386981285212977
70-74	0.009796335602581122
75-79	0.011926061259728081
80-84	0.0031472027165328708
85-89	7.099061007200578E-4
90-94	0.0011831981135089278
95-99	2.839720495777336E-4
100-104	0.0026268129446502114
105-109	4.023233463267997E-4
110-114	3.313486339087518E-4
115-119	4.7341356746474723E-4
120-124	0.001065455173104502
125-129	0.022499341888310133
130-134	0.016442398067839765
135-139	0.007186113193180331
140-144	0.005282507049291502
145-149	0.004283113823749866
150-154	0.006043953821337393
155-159	0.00830449958727351
160-164	0.010660247674673988
165-169	0.008637699202476236
170-174	0.00602023894875682
175-179	0.008733005143668643
180-184	0.0036408208837485894
185-189	0.004093204162598252
190-194	0.0034510383341338157
195-199	0.002475645244797277
200-204	0.0022860626381162844
205-209	2.1448717902887356E-4
210-214	6.680573393614374E-4
215-219	5.258518321633927E-4
220-224	6.231996122739642E-4
225-229	3.8526116011285263E-4
230-234	3.154845138359374E-4
235-239	0.0
240-244	0.0
245-249	5.3346264467840335E-5
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	16.0
80-84	10.0
85-89	14.0
90-94	13.0
95-99	17.0
100-104	34.0
105-109	48.0
110-114	80.0
115-119	143.0
120-124	542.0
125-129	3787.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	3.0
160-164	3.0
165-169	3.0
170-174	6.0
175-179	13.0
180-184	38.0
185-189	81.0
190-194	104.0
195-199	211.0
200-204	548.0
205-209	781.0
210-214	1284.0
215-219	1901.0
220-224	3129.0
225-229	5112.0
230-234	8770.0
235-239	14123.0
240-244	28453.0
245-249	101708.0
250-253	674238.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.44929663969371
#Duplication Level	Percentage of total
1	39.47175928097434
2	19.334631277344574
3	9.516956506293473
4	5.584034100805653
5	3.8401379301716743
6	3.0001000680677907
7	2.169583912164347
8	1.8661038719420966
9	1.5159852898641832
>10	12.192796888463324
>50	1.2059719378790623
>100	0.3019389360294783
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.549396957456928E-4	0.0	1.1831323191523093E-4
6	0.0	0.0	0.0	3.549396957456928E-4	0.0	1.1831323191523093E-4
7	0.0	0.0	0.0	3.549396957456928E-4	0.0	1.1831323191523093E-4
8	0.0	0.0	0.0	3.549396957456928E-4	0.0	1.1831323191523093E-4
9	0.0	0.0	0.0	3.549396957456928E-4	0.0	1.1831323191523093E-4
10-14	0.0	0.0	0.0	5.442408668100624E-4	0.0	1.1831323191523093E-4
15-19	0.0	0.0	0.0	5.915661595761547E-4	7.098793914913856E-5	1.1831323191523093E-4
20-24	0.0	0.0	0.0	8.991805625557551E-4	1.1831323191523093E-4	1.1831323191523093E-4
25-29	0.0	0.0	0.0	0.01315643138897368	1.1831323191523093E-4	1.1831323191523093E-4
30-34	0.0	0.0	0.0	0.15113332244851602	3.3127704936264656E-4	1.1831323191523093E-4
35-39	0.0	0.0	0.0	0.5619405263045809	3.549396957456928E-4	1.1831323191523093E-4
40-44	0.0	0.0	0.0	1.5233065235549814	3.549396957456928E-4	1.656385246813233E-4
45-49	0.0	0.0	0.0	2.76826933770619	3.78602342128739E-4	2.8395175659655424E-4
50-54	0.0	0.0	0.0	4.166826389529752	0.001135807026386217	3.549396957456928E-4
55-59	0.0	0.0	0.0	5.662258315645506	0.001893011710643695	3.549396957456928E-4
60-64	0.0	0.0	0.0	7.244934418975549	0.0038806740068195746	3.549396957456928E-4
65-69	0.0	0.0	0.0	8.85695220382057	0.0050874689723549305	3.549396957456928E-4
70-74	0.0	0.0	0.0	10.570080476660348	0.005726360424697177	3.549396957456928E-4
75-79	0.0	0.0	0.0	12.30211520396018	0.006128625413208962	4.0226498851178517E-4
80-84	0.0	0.0	0.0	14.059374312304339	0.0064599024625716095	4.7325292766092373E-4
85-89	0.0	0.0	0.0	15.81322599956934	0.0069568180366155795	4.7325292766092373E-4
90-94	0.0	0.0	0.0	17.574247468688405	0.007548384196191733	4.7325292766092373E-4
95-99	0.0	0.0	0.0	19.365770088995212	0.020397201182185815	4.7325292766092373E-4
100-104	0.0	0.0	0.0	21.155399697591378	0.05821011010229362	5.442408668100624E-4
105-109	0.0	0.0	0.0	22.957901785819924	0.15342859914767146	5.915661595761547E-4
110-114	0.0	0.0	0.0	24.775382329208934	0.2673879041284219	5.915661595761547E-4
115-119	0.0	0.0	2.3662646383046187E-5	25.882084300544005	0.3578502012508075	5.915661595761547E-4
120-124	0.0	0.0	1.1831323191523093E-4	25.918098848339003	0.36724427186487685	5.915661595761547E-4
125-129	0.0	0.0	1.1831323191523093E-4	25.918737739791347	0.36724427186487685	5.915661595761547E-4
130-134	0.0	0.0	1.1831323191523093E-4	25.919329305950917	0.36724427186487685	5.915661595761547E-4
135-139	0.0	0.0	1.1831323191523093E-4	25.920062847988795	0.36743357303594115	5.915661595761547E-4
140-144	0.0	0.0	1.1831323191523093E-4	25.921316968247094	0.3677648500853038	5.915661595761547E-4
145-149	0.0	0.0	1.1831323191523093E-4	25.923517594360714	0.36807246448828346	5.915661595761547E-4
150-154	0.0	0.0	1.1831323191523093E-4	25.92685402750073	0.36826176565934776	5.915661595761547E-4
155-159	0.0	0.0	1.1831323191523093E-4	25.931444580899033	0.3685693800623274	5.915661595761547E-4
160-164	0.0	0.0	1.1831323191523093E-4	25.93795180865438	0.36890065711169007	6.388914523422469E-4
165-169	0.0	0.0	1.1831323191523093E-4	25.947061927511847	0.36925559680743575	7.098793914913856E-4
170-174	0.0	0.0	1.1831323191523093E-4	25.957307853395708	0.3694448979785001	7.098793914913856E-4
175-179	0.0	0.0	1.1831323191523093E-4	25.969778068039574	0.3694922232712662	7.57204684257478E-4
180-184	0.0	0.0	1.1831323191523093E-4	25.990530208917505	0.3694922232712662	8.281926234066166E-4
185-189	0.0	0.0	1.1831323191523093E-4	26.012205193004377	0.3694922232712662	8.281926234066166E-4
190-194	0.0	0.0	1.1831323191523093E-4	26.03513429734955	0.36956321121041535	8.281926234066166E-4
195-199	0.0	0.0	1.1831323191523093E-4	26.057897763170036	0.3700601267844593	8.281926234066166E-4
200-204	0.0	0.0	1.1831323191523093E-4	26.082246626298193	0.37325458404617057	8.281926234066166E-4
205-209	0.0	0.0	1.1831323191523093E-4	26.109364019053164	0.3828142931849212	8.991805625557551E-4
210-214	0.0	0.0	1.1831323191523093E-4	26.131654231945994	0.39869192890794525	9.465058553218475E-4
215-219	0.0	0.0	1.1831323191523093E-4	26.154323047180952	0.42457886405099776	0.0010174937944709861
220-224	0.0	0.0	1.1831323191523093E-4	26.172282995785686	0.47029509686304294	0.0011594696727692633
225-229	0.0	0.0	1.1831323191523093E-4	26.195306750716384	0.5315340257023665	0.001254120258301448
230-234	0.0	0.0	1.1831323191523093E-4	26.223488962558594	0.5838521368552817	0.0013014455510675404
235-239	0.0	0.0	1.1831323191523093E-4	26.255386209882943	0.6182339620498478	0.0013014455510675404
240-241	0.0	0.0	1.1831323191523093E-4	26.302628683386693	0.6194289256921917	0.0013014455510675404
>>END_MODULE
