##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665010
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1873556
Total Bases	465.3 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.702170097931422	30.0	18.0	32.0	18.0	33.0
2	26.91163274543168	29.0	25.0	31.0	18.0	33.0
3	25.96144017045661	28.0	18.0	31.0	18.0	33.0
4	30.3195431575037	31.0	29.0	33.0	27.0	33.0
5	31.855534609053585	33.0	32.0	33.0	30.0	33.0
6	35.87489511922782	37.0	36.0	38.0	31.0	38.0
7	36.083070375265	38.0	36.0	38.0	33.0	38.0
8	36.64828539952902	38.0	37.0	38.0	34.0	38.0
9	36.8463510031192	38.0	38.0	38.0	35.0	38.0
10-14	36.895373823894246	38.0	38.0	38.0	35.0	38.0
15-19	36.84620934735871	38.0	38.0	38.0	35.0	38.0
20-24	36.85045304223626	38.0	38.0	38.0	35.0	38.0
25-29	36.79596873538875	38.0	38.0	38.0	35.0	38.0
30-34	36.77343564857416	38.0	38.0	38.0	35.0	38.0
35-39	36.717539801318985	38.0	38.0	38.0	34.4	38.0
40-44	36.750293452664344	38.0	38.0	38.0	35.0	38.0
45-49	36.71017679749097	38.0	38.0	38.0	34.2	38.0
50-54	36.61265497268297	38.0	38.0	38.0	34.0	38.0
55-59	36.513262373796145	38.0	38.0	38.0	34.0	38.0
60-64	36.502311219947515	38.0	38.0	38.0	34.0	38.0
65-69	36.44108241226844	38.0	38.0	38.0	34.0	38.0
70-74	36.369556607862265	38.0	37.4	38.0	33.6	38.0
75-79	36.28229090259597	38.0	37.0	38.0	33.0	38.0
80-84	36.17834607046084	38.0	37.0	38.0	33.0	38.0
85-89	36.068890453429375	38.0	37.0	38.0	33.0	38.0
90-94	35.98500334844549	38.0	37.0	38.0	31.6	38.0
95-99	35.90016578807323	38.0	37.0	38.0	31.0	38.0
100-104	35.789996240949534	38.0	37.0	38.0	31.0	38.0
105-109	35.63622153359887	38.0	36.0	38.0	30.4	38.0
110-114	35.52575671203865	38.0	36.0	38.0	29.4	38.0
115-119	35.36711559663591	38.0	35.6	38.0	29.0	38.0
120-124	35.47032942663939	38.0	35.0	38.0	29.8	38.0
125-129	33.45967825463358	34.4	33.2	35.6	29.8	35.6
130-134	34.71150871303429	36.2	35.4	36.8	30.8	37.2
135-139	35.93426851536545	38.0	37.0	38.0	31.0	38.0
140-144	35.83665797016734	38.0	37.0	38.0	30.4	38.0
145-149	35.82586426489554	38.0	37.0	38.0	30.6	38.0
150-154	35.79922745054927	38.0	37.0	38.0	29.6	38.0
155-159	35.78666997243172	38.0	37.0	38.0	29.2	38.0
160-164	35.743823169104324	38.0	37.0	38.0	29.0	38.0
165-169	35.718573818723996	38.0	37.0	38.0	29.0	38.0
170-174	35.68592567555381	38.0	37.0	38.0	29.0	38.0
175-179	35.646652544065766	38.0	37.0	38.0	29.0	38.0
180-184	35.535471263701126	38.0	37.0	38.0	29.0	38.0
185-189	35.46181237705307	38.0	36.2	38.0	29.0	38.0
190-194	35.377204233293995	38.0	36.0	38.0	28.2	38.0
195-199	35.26174077095312	38.0	36.0	38.0	28.0	38.0
200-204	35.10034764105325	38.0	36.0	38.0	27.0	38.0
205-209	34.953797417477695	38.0	36.0	38.0	26.2	38.0
210-214	34.784193535088306	38.0	35.4	38.0	25.0	38.0
215-219	34.567471942511126	38.0	35.0	38.0	24.6	38.0
220-224	34.37883628701454	38.0	34.6	38.0	23.0	38.0
225-229	34.130341989923444	38.0	34.0	38.0	23.0	38.0
230-234	33.96191817187238	38.0	34.0	38.0	21.6	38.0
235-239	33.80349951222744	38.0	34.0	38.0	21.0	38.0
240-244	33.81796722222033	38.0	34.0	38.0	21.0	38.0
245-249	34.19504456135432	38.0	33.8	38.0	23.4	38.0
250-252	35.682089481074534	37.666666666666664	34.666666666666664	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	4.0
18	15.0
19	38.0
20	119.0
21	475.0
22	1566.0
23	3680.0
24	6668.0
25	11005.0
26	16069.0
27	21746.0
28	29024.0
29	36082.0
30	44706.0
31	55011.0
32	69919.0
33	90985.0
34	128831.0
35	201458.0
36	379376.0
37	776776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.31518353464961	21.306982050306996	13.412919848062108	17.964914566981278
2	18.216642577003302	20.82553176953344	41.99233970054805	18.965485952915206
3	14.91287156615548	33.88262747417211	33.94907865043799	17.25542230923442
4	10.250347467596379	22.498446803831857	47.39036356532711	19.860842163244655
5	9.605530872842873	41.14774258148676	39.54848427268787	9.6982422729825
6	19.763113565860856	47.463753418632805	23.10803626899863	9.665096746507711
7	18.40676232789412	34.75177683506658	32.675297669245005	14.166163167794291
8	17.594937114236245	39.59860287069081	22.992213736872557	19.814246278200383
9	19.79561859907043	17.949503510970583	25.72829421698631	36.52658367297268
10-14	20.5721419589273	26.257448402930045	32.163938521186445	21.006471116956206
15-19	20.1372150071842	31.40828456688778	28.938307688694653	19.516192737233368
20-24	20.010961014527933	29.975577903158346	30.055223529758386	19.95823755255533
25-29	20.29249473456103	30.16752262297322	29.59269574462861	19.94728689783714
30-34	19.99195113463382	30.02139247505812	29.928424877612414	20.05823151269564
35-39	20.15853275802805	29.618031166402286	29.967676439882236	20.255759635687433
40-44	20.104282978464482	29.033559712119626	30.516440394629253	20.345716914786642
45-49	19.245501069789928	29.073957855518206	30.879070237636085	20.801470837055778
50-54	19.124727093697867	28.998469632408607	30.68997630577633	21.186826968117202
55-59	18.977085719214553	28.46780095836632	30.47353177033027	22.081581552088856
60-64	18.951392815689214	28.63667316530252	29.941467442005198	22.47046657700307
65-69	19.187300792926486	28.65768926893773	29.09542841483772	23.059581523298068
70-74	19.35539351385768	28.949737916497543	28.350455040332612	23.344413529312163
75-79	19.67451541955335	29.1535097004701	27.784469916921744	23.387504963054802
80-84	19.558908303656068	29.24230099151956	27.69148241141657	23.507308293407807
85-89	19.791456610786152	28.595202137763494	27.835600931599274	23.777740319851077
90-94	19.79550723559316	28.232525810598126	27.923613162409566	24.048353791399148
95-99	19.665362908899294	27.65103046967092	28.238650229597017	24.444956391832765
100-104	19.90452620805126	27.70760943032697	28.217059924355908	24.170804437265865
105-109	19.481688773040975	28.79339906044039	27.846429909431713	23.878482257086922
110-114	19.25860830893011	29.889639597553714	27.183468629958167	23.668283463558005
115-119	19.299610919874613	30.311661382387843	26.909218054889863	23.479509642847685
120-124	19.148685918798172	30.711192495426943	27.131455338374426	23.008666247400463
125-129	20.99355136647064	29.21835832801617	30.4249605029902	19.363129802522987
130-134	16.028261156635775	35.78741721378327	32.02184111160921	16.16248051797174
135-139	20.67907276381817	25.631915171359438	31.373397237701745	22.31561482712065
140-144	19.920079673625597	30.161841074285363	29.223187513508208	20.694891738580836
145-149	19.9668888378808	29.859757549495125	30.209339342753623	19.96401426987045
150-154	19.9605212829607	29.921287122447094	29.95096641659689	20.16722517799532
155-159	19.777215424498067	29.7085145034801	29.993549098504214	20.520720973517623
160-164	19.942039382627282	29.354638120503036	30.170328098456462	20.53299439841322
165-169	19.91680321485713	29.299262146353062	30.149647377438075	20.634287261351737
170-174	19.54113681350778	29.281231758560082	30.16495142159074	21.012680006341398
175-179	19.846743434753865	28.721441384464335	30.084289287347428	21.347525893434373
180-184	19.847666415880898	28.51979115916873	29.95070740251374	21.681835022436637
185-189	19.513333802480876	28.10705832476567	30.537535808258255	21.8420720644952
190-194	19.669492477409484	28.093740253227516	30.178566504652753	22.058200764710243
195-199	19.410914126193006	27.901584552371816	30.383242495509265	22.30425882592591
200-204	19.947710952658802	27.559844099423508	29.749909760368	22.742535187549688
205-209	20.19519666420321	26.91125721755035	29.995798430453597	22.89774768779285
210-214	20.34167304804833	26.968977062455195	29.647197449012392	23.04215244048408
215-219	20.30274228563405	26.94486266970525	29.31372654908356	23.438668495577133
220-224	20.59579323210935	26.551424103452337	29.855068373141	22.997714291297317
225-229	20.500161672508945	27.026303652157356	29.352002359160267	23.121532316173433
230-234	20.64773034589294	27.189726084987818	29.166501201443907	22.996042367675333
235-239	20.95395931530778	26.896215676575792	29.32138731140283	22.828437696713596
240-244	20.80199000170186	26.903760168366723	28.796186337972106	23.498063491959307
245-249	20.506422208815543	27.554884409815518	29.166713382878136	22.771979998490803
250-252	19.215844073294953	27.91211941276439	29.756006945031753	23.116029568908907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.3333333333333333
13	0.3333333333333333
14	0.3333333333333333
15	3.666666666666667
16	15.666666666666677
17	42.66666666666669
18	69.0000000000001
19	81.99999999999991
20	119.66666666666605
21	160.50000000000017
22	265.50000000000244
23	416.66666666666055
24	671.0000000000059
25	1135.8333333333558
26	1940.3333333331734
27	3212.1666666670008
28	5388.16666666627
29	8111.666666663834
30	11740.333333336595
31	17106.5000000098
32	23555.83333331977
33	31205.33333329212
34	39707.666666667275
35	48894.5000000672
36	57638.00000012992
37	64643.50000018056
38	67393.50000016954
39	68396.6666668224
40	66227.66666685489
41	61369.33333348999
42	54870.00000010955
43	47843.66666672554
44	39671.499999999854
45	32300.99999995562
46	25542.499999980068
47	20001.99999999986
48	15931.166666677243
49	13007.000000005375
50	10833.166666668127
51	9180.999999998512
52	8011.999999997332
53	7435.166666664495
54	6923.833333331602
55	6561.166666665278
56	6293.333333332213
57	6183.499999998974
58	6012.166666665761
59	5880.166666665853
60	5524.999999999513
61	5056.999999999938
62	4492.000000000438
63	4008.8333333340165
64	3566.6666666671513
65	3029.000000000244
66	2613.5000000000564
67	2251.833333333226
68	1874.1666666665244
69	1540.9999999999318
70	1264.6666666666622
71	1032.5000000000457
72	786.8333333333518
73	571.3333333333277
74	412.49999999999426
75	298.8333333333342
76	195.16666666666768
77	103.83333333333296
78	72.16666666666673
79	52.66666666666676
80	25.499999999999982
81	9.33333333333333
82	5.333333333333332
83	0.6666666666666666
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06468981978654494
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	3.629461836208792E-4
25-29	9.607398978199745E-4
30-34	0.0
35-39	0.0
40-44	0.0
45-49	5.657690509384294E-4
50-54	0.001024789224341306
55-59	0.0018360806936115065
60-64	0.0018467555813650618
65-69	0.0035120380709196845
70-74	0.009799546957763739
75-79	0.011123265098898317
80-84	0.0027754945183983263
85-89	5.444298527754927E-4
90-94	0.0011635997806027276
95-99	2.135086495556565E-4
100-104	0.002626239657179643
105-109	4.484139757824423E-4
110-114	4.377878855763413E-4
115-119	5.12619408283417E-4
120-124	0.00154898907631538
125-129	0.02179963192365951
130-134	0.01739657377589845
135-139	0.008430152273647462
140-144	0.006864246126125161
145-149	0.005738080746057751
150-154	0.00726108535529177
155-159	0.010114041990542137
160-164	0.011969557425614188
165-169	0.009556387350946903
170-174	0.007282637666252482
175-179	0.009921207379833801
180-184	0.004150986502858228
185-189	0.004988019634990668
190-194	0.0040232736186546
195-199	0.003230368641942291
200-204	0.0022874860978837853
205-209	1.720189281027538E-4
210-214	4.630540980718427E-4
215-219	3.7802140357187024E-4
220-224	5.427918062320099E-4
225-229	3.8297909590671945E-4
230-234	1.6638933262458873E-4
235-239	0.0
240-244	1.1720783281193429E-5
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	10.0
80-84	17.0
85-89	22.0
90-94	31.0
95-99	42.0
100-104	95.0
105-109	176.0
110-114	278.0
115-119	425.0
120-124	1085.0
125-129	6640.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	2.0
160-164	9.0
165-169	10.0
170-174	11.0
175-179	45.0
180-184	120.0
185-189	221.0
190-194	461.0
195-199	792.0
200-204	1916.0
205-209	2488.0
210-214	4113.0
215-219	7838.0
220-224	11958.0
225-229	19737.0
230-234	32620.0
235-239	47471.0
240-244	82224.0
245-249	238520.0
250-253	1414179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.368523027515
#Duplication Level	Percentage of total
1	21.840780250324386
2	7.9444533314224675
3	4.940444004098334
4	3.8942064532395597
5	3.3108855634365795
6	2.9581673056928244
7	2.696455269450658
8	2.448788563641178
9	2.1737399449435917
>10	31.888798643412386
>50	7.938723534462221
>100	7.155445437266479
>500	0.629303122630439
>1k	0.1798085759789365
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	5.337443876777636E-5	0.0	0.0
2	0.0	0.0	0.0	5.337443876777636E-5	0.0	0.0
3	0.0	0.0	0.0	5.337443876777636E-5	0.0	0.0
4	0.0	0.0	0.0	5.337443876777636E-5	0.0	0.0
5	0.0	0.0	0.0	5.337443876777636E-5	0.0	0.0
6	0.0	0.0	0.0	5.337443876777636E-5	0.0	0.0
7	0.0	0.0	0.0	5.337443876777636E-5	0.0	0.0
8	0.0	0.0	0.0	5.337443876777636E-5	0.0	0.0
9	0.0	0.0	0.0	5.337443876777636E-5	0.0	5.337443876777636E-5
10-14	0.0	0.0	0.0	5.337443876777635E-5	5.337443876777635E-5	5.337443876777635E-5
15-19	0.0	0.0	0.0	5.337443876777635E-5	1.6012331630332907E-4	5.337443876777635E-5
20-24	0.0	0.0	0.0	5.764439386919847E-4	1.6012331630332907E-4	5.337443876777635E-5
25-29	0.0	0.0	0.0	0.015873558089536687	1.6012331630332907E-4	5.337443876777635E-5
30-34	0.0	0.0	0.0	0.36501711184506896	2.134977550711054E-4	5.337443876777635E-5
35-39	1.0674887753555272E-5	0.0	0.0	1.3788432264634736	3.095717448531029E-4	5.337443876777635E-5
40-44	5.337443876777635E-5	0.0	0.0	3.4808246991282887	3.2024663260665813E-4	9.607398978199745E-5
45-49	5.337443876777635E-5	0.0	0.0	5.840028267102772	3.5227129586732394E-4	1.067488775355527E-4
50-54	5.337443876777635E-5	0.0	0.0	8.463339232987966	5.12394612170653E-4	1.067488775355527E-4
55-59	5.337443876777635E-5	0.0	0.0	11.013249670679713	6.191434897062058E-4	1.067488775355527E-4
60-64	5.337443876777635E-5	0.0	0.0	13.72359299641964	0.0013343609691944091	1.067488775355527E-4
65-69	5.337443876777635E-5	0.0	0.0	16.37346308303568	0.001611908050786846	1.067488775355527E-4
70-74	5.337443876777635E-5	0.0	0.0	19.104174094609395	0.0016546076018010669	1.1742376528910798E-4
75-79	5.337443876777635E-5	0.0	0.0	21.789164561934633	0.0018147309181043962	1.6012331630332907E-4
80-84	5.337443876777635E-5	0.0	0.0	24.544107568708917	0.0019748542344077253	1.6012331630332907E-4
85-89	5.337443876777635E-5	0.0	0.0	27.298997201044433	0.002220376652739496	1.6012331630332907E-4
90-94	5.337443876777635E-5	0.0	0.0	30.027338387536854	0.003277190540341468	1.6012331630332907E-4
95-99	5.337443876777635E-5	0.0	0.0	32.70173936621057	0.04061794790227781	1.6012331630332907E-4
100-104	5.337443876777635E-5	0.0	0.0	35.273672097337894	0.1718123183934721	1.6012331630332907E-4
105-109	5.337443876777635E-5	0.0	0.0	37.76420880934437	0.45861452766824157	1.6012331630332907E-4
110-114	5.337443876777635E-5	0.0	0.0	40.18977815448271	0.7402073917192761	1.6012331630332907E-4
115-119	5.337443876777635E-5	0.0	3.2024663260665815E-5	41.66602973169737	0.9588290929120881	1.6012331630332907E-4
120-124	5.337443876777635E-5	0.0	5.337443876777635E-5	41.71836870635305	0.9816520029291892	1.6012331630332907E-4
125-129	5.337443876777635E-5	0.0	5.337443876777635E-5	41.71869962787341	0.9816626778169427	1.6012331630332907E-4
130-134	5.337443876777635E-5	0.0	5.337443876777635E-5	41.71912662338356	0.9816626778169427	1.921479795639949E-4
135-139	5.337443876777635E-5	0.0	5.337443876777635E-5	41.72025816148543	0.9816840275924499	2.134977550711054E-4
140-144	5.337443876777635E-5	0.0	5.337443876777635E-5	41.72289485876056	0.9817267271434641	2.134977550711054E-4
145-149	5.337443876777635E-5	0.0	5.337443876777635E-5	41.72642824660698	0.9818228011332462	2.134977550711054E-4
150-154	5.337443876777635E-5	0.0	5.337443876777635E-5	41.731456118738905	0.9819935993373029	2.2417264282466068E-4
155-159	5.337443876777635E-5	0.0	5.337443876777635E-5	41.7386509930848	0.982089673327085	2.6687219383888177E-4
160-164	5.337443876777635E-5	0.0	5.337443876777635E-5	41.747575199246775	0.9822284468678812	2.6687219383888177E-4
165-169	5.337443876777635E-5	0.0	5.337443876777635E-5	41.760555862755105	0.9823565455209238	2.6687219383888177E-4
170-174	5.337443876777635E-5	0.0	5.337443876777635E-5	41.776525494834424	0.9825486935004879	2.6687219383888177E-4
175-179	5.337443876777635E-5	0.0	5.337443876777635E-5	41.798088768096605	0.9826767921535305	3.095717448531029E-4
180-184	5.337443876777635E-5	0.0	5.337443876777635E-5	41.826942989694466	0.9827301665922983	3.2024663260665813E-4
185-189	5.337443876777635E-5	0.0	5.337443876777635E-5	41.85476174718023	0.9827728661433126	3.2024663260665813E-4
190-194	5.337443876777635E-5	0.0	5.337443876777635E-5	41.88309289927817	0.9831037876636728	3.2024663260665813E-4
195-199	5.337443876777635E-5	0.0	5.337443876777635E-5	41.913132033416666	0.9839044042451894	3.2024663260665813E-4
200-204	5.337443876777635E-5	0.0	5.337443876777635E-5	41.94184748147373	0.9876726396221944	3.2024663260665813E-4
205-209	5.337443876777635E-5	0.0	5.337443876777635E-5	41.974074967601716	1.011082668465741	3.2024663260665813E-4
210-214	5.337443876777635E-5	0.0	5.337443876777635E-5	42.005800734005284	1.0565897149591472	3.2024663260665813E-4
215-219	5.337443876777635E-5	0.0	5.337443876777635E-5	42.03327789508293	1.113839137981464	3.2024663260665813E-4
220-224	5.337443876777635E-5	0.0	5.337443876777635E-5	42.054414172834974	1.2136920380282201	3.2024663260665813E-4
225-229	5.337443876777635E-5	0.0	5.337443876777635E-5	42.0765859146991	1.3383000027754708	3.2024663260665813E-4
230-234	5.337443876777635E-5	0.0	5.337443876777635E-5	42.105493510735734	1.4408429745361224	3.2024663260665813E-4
235-239	5.337443876777635E-5	0.0	5.337443876777635E-5	42.14132910892442	1.503942236047388	3.2024663260665813E-4
240-241	5.337443876777636E-5	0.0	5.337443876777636E-5	42.20485002850195	1.5064935342204877	3.2024663260665813E-4
>>END_MODULE
