##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665014
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4668874
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.28543434669687	30.0	18.0	32.0	18.0	33.0
2	27.146095825246086	29.0	25.0	31.0	18.0	33.0
3	26.38961085692182	29.0	18.0	31.0	18.0	33.0
4	30.1438453897021	31.0	29.0	33.0	27.0	33.0
5	31.674291060328464	33.0	32.0	33.0	29.0	33.0
6	35.622033706628194	37.0	36.0	38.0	30.0	38.0
7	35.98648667751582	38.0	36.0	38.0	33.0	38.0
8	36.54750031806384	38.0	37.0	38.0	34.0	38.0
9	36.765696825401584	38.0	38.0	38.0	34.0	38.0
10-14	36.82819493522421	38.0	38.0	38.0	34.8	38.0
15-19	36.778934064187645	38.0	38.0	38.0	34.6	38.0
20-24	36.777815764571926	38.0	38.0	38.0	34.6	38.0
25-29	36.72181399626548	38.0	38.0	38.0	34.2	38.0
30-34	36.692871557467605	38.0	38.0	38.0	34.0	38.0
35-39	36.63085930354942	38.0	38.0	38.0	34.0	38.0
40-44	36.64900295017599	38.0	38.0	38.0	34.0	38.0
45-49	36.599606029205326	38.0	38.0	38.0	34.0	38.0
50-54	36.49347037422727	38.0	38.0	38.0	34.0	38.0
55-59	36.387636547912834	38.0	37.6	38.0	33.6	38.0
60-64	36.36804968392808	38.0	37.6	38.0	33.6	38.0
65-69	36.3053495553746	38.0	37.0	38.0	33.0	38.0
70-74	36.211025784803795	38.0	37.0	38.0	33.0	38.0
75-79	36.10251039912686	38.0	37.0	38.0	32.8	38.0
80-84	35.969122476509895	38.0	37.0	38.0	31.4	38.0
85-89	35.83609936072343	38.0	37.0	38.0	31.0	38.0
90-94	35.72490448144228	38.0	36.8	38.0	31.0	38.0
95-99	35.60368108077522	38.0	36.0	38.0	30.2	38.0
100-104	35.4689134934417	38.0	36.0	38.0	29.0	38.0
105-109	35.27116924335125	38.0	35.2	38.0	28.2	38.0
110-114	35.14846458065722	38.0	35.0	38.0	28.0	38.0
115-119	34.990888929518135	38.0	35.0	38.0	27.8	38.0
120-124	35.12887230138568	38.0	35.0	38.0	28.8	38.0
125-129	33.32404875345145	34.4	33.0	35.6	29.4	35.6
130-134	34.546745810692	36.2	35.4	37.0	29.4	37.8
135-139	35.70951681864504	38.0	37.0	38.0	29.0	38.0
140-144	35.60877349426355	38.0	37.0	38.0	29.0	38.0
145-149	35.580909504384934	38.0	37.0	38.0	29.0	38.0
150-154	35.5523633675994	38.0	37.0	38.0	29.0	38.0
155-159	35.54566863067534	38.0	37.0	38.0	29.0	38.0
160-164	35.50055889750642	38.0	37.0	38.0	28.8	38.0
165-169	35.45764894500545	38.0	36.4	38.0	28.6	38.0
170-174	35.407887427774995	38.0	36.0	38.0	28.0	38.0
175-179	35.350637355439645	38.0	36.0	38.0	28.0	38.0
180-184	35.217252328938244	38.0	36.0	38.0	27.6	38.0
185-189	35.107751105901556	38.0	36.0	38.0	27.0	38.0
190-194	34.99092344271241	38.0	36.0	38.0	27.0	38.0
195-199	34.840630965933975	38.0	35.2	38.0	25.8	38.0
200-204	34.633369247594956	38.0	35.0	38.0	25.0	38.0
205-209	34.44952055467311	38.0	34.8	38.0	23.2	38.0
210-214	34.23075591407223	38.0	34.0	38.0	23.0	38.0
215-219	33.96243099632872	38.0	34.0	38.0	21.8	38.0
220-224	33.729711623931756	38.0	34.0	38.0	21.0	38.0
225-229	33.42684484426035	38.0	33.4	38.0	15.0	38.0
230-234	33.225504304258514	38.0	33.0	38.0	15.0	38.0
235-239	33.03323296010952	38.0	32.4	38.0	15.0	38.0
240-244	33.06550151824743	38.0	32.0	38.0	16.0	38.0
245-249	33.515826972145945	38.0	32.6	38.0	21.8	38.0
250-252	35.276534438486145	37.333333333333336	33.666666666666664	38.0	30.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	0.0
7	0.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	0.0
15	1.0
16	3.0
17	7.0
18	25.0
19	97.0
20	498.0
21	2086.0
22	6210.0
23	13383.0
24	23452.0
25	35545.0
26	50019.0
27	66416.0
28	84500.0
29	104511.0
30	127264.0
31	154963.0
32	192180.0
33	250047.0
34	347690.0
35	539149.0
36	995297.0
37	1675527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57142422341956	19.021746352709386	12.860158293248466	22.54667113062258
2	18.61626148915613	18.585204650643767	36.3335881421992	26.46494571800091
3	18.423740089126646	26.409304879861516	29.346958637886488	25.81999639312535
4	13.987795315476168	19.333229525047106	40.508235088500136	26.170740070976596
5	14.538654370742005	36.878756802511276	34.50188311332264	14.08070571342408
6	26.49884736588353	40.27166310656259	19.999995716309147	13.22949381124473
7	25.641150856631263	30.53132770884604	26.730964880153014	17.096556554369688
8	23.621266735048682	34.96285717161908	20.541355947232617	20.874520146099623
9	23.784400982593006	16.24202082259287	22.43488843448448	37.53868976032964
10-14	24.167934603787838	23.281304610379156	27.839701162795333	24.711059623037677
15-19	24.16173285285158	26.821545840560635	25.487796874858105	23.528924431729685
20-24	23.931650439389056	25.946730652779664	26.113272863292313	24.008346044538968
25-29	24.074780178432643	25.985695625098764	26.009517544237294	23.930006652231295
30-34	23.89296422752491	25.982116868132305	26.105414394792398	24.019504509550384
35-39	23.88540350992928	25.464308379609708	26.31960331292225	24.330684797538762
40-44	23.50707558788315	25.38280140590764	26.576970444668625	24.533152561540586
45-49	22.960707630031578	24.916673006577746	27.370758342466235	24.751861020924448
50-54	22.43625370126879	25.584502961828942	26.973556656175184	25.005686680727084
55-59	22.091888651500767	25.057648987129895	27.215016949637555	25.635445411731787
60-64	22.240847117945652	25.174311032122134	26.247743308060212	26.337098541872
65-69	22.25249443375238	25.25062572359934	25.982717836761733	26.514162005886554
70-74	22.69731609505413	25.607853353727673	25.305588397248147	26.38924215397005
75-79	22.915801163313624	25.646576792412905	25.30801137394042	26.129610670333054
80-84	23.027567956012934	25.883013165490198	25.036896144384507	26.052522734112358
85-89	23.007096295261196	25.336995095312894	25.423245757111303	26.232662852314615
90-94	23.045797709444095	24.85897418706805	25.630805069445245	26.46442303404261
95-99	22.738671670606376	24.683740174754615	26.101885328133356	26.475702826505653
100-104	22.70781659430928	24.809064154084346	26.360225912367575	26.1228933392388
105-109	22.26476617401062	26.169042111099785	25.80096294610039	25.765228768789207
110-114	21.912209485104977	27.35428872340579	25.534026981874607	25.19947480961463
115-119	21.718679800187747	27.854115260480327	25.34319074707721	25.084014192254717
120-124	21.698542891031277	28.11503403374795	25.359062130846453	24.82736094437432
125-129	22.89947849537367	25.407569727416877	27.240604036723088	24.452347740486363
130-134	21.69134176321407	31.106525048262906	27.503992905952924	19.6981402825701
135-139	24.41079861586054	22.8090488911548	27.254256698596656	25.525895794388003
140-144	23.705637154242762	25.78353535643119	25.90411711939805	24.606710369927992
145-149	23.686530990075862	25.85037350172411	26.4237892631234	24.039306245076627
150-154	23.73184767885125	25.75161108940545	26.40636597545663	24.110175256286666
155-159	23.510728341386734	25.7088184526237	26.425815808043428	24.354637397946142
160-164	23.528850700029142	25.370774727706475	26.63030904910459	24.470065523159793
165-169	23.366234589410027	25.569370916757663	26.215422917149716	24.848971576682594
170-174	23.265463286341905	25.348817488507176	26.551993442638803	24.833725782512115
175-179	23.35617736973612	25.233294021906943	26.27152846806899	25.139000140287948
180-184	23.27160470372365	24.838211485179706	26.723693196125847	25.166490614970794
185-189	22.904837999593646	24.52142843725244	27.176197289870384	25.397536273283528
190-194	22.81683328557655	24.616312329188876	27.12027375218955	25.44658063304503
195-199	22.62483228201761	24.54180922866213	27.31014168834165	25.52321680097861
200-204	22.954920459788728	24.180291693355798	27.076600503565608	25.788187343289863
205-209	23.20949004191828	23.752067110826594	27.12307116578554	25.91537168146959
210-214	23.11726519820361	24.037658907489813	26.797246629599503	26.04782926470707
215-219	22.98201382325894	24.174614781863628	26.47947081588829	26.363900578989142
220-224	23.191705797372308	24.05910956770223	27.31718138127799	25.432003253647473
225-229	22.911399331232047	24.493905691054604	26.91747563775146	25.677219339961894
230-234	23.099918557888387	24.837548691650063	26.611498579949007	25.451034170512543
235-239	23.23448246970466	24.521578160553208	27.151577264833705	25.092362104908428
240-244	23.168192968580424	24.485922872191523	26.586481383775055	25.759402775453
245-249	22.617284430553404	25.265847335931817	27.01361748379537	25.103250749719408
250-252	21.872438504430434	25.188453176328697	26.580306889828776	26.3588014294121
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.3333333333333333
15	0.6666666666666666
16	2.333333333333333
17	4.166666666666667
18	7.499999999999998
19	18.500000000000007
20	32.49999999999996
21	48.33333333333339
22	82.49999999999993
23	162.00000000000014
24	314.5000000000001
25	561.9999999999924
26	951.3333333333695
27	1467.1666666666188
28	2316.999999999918
29	3571.5000000004857
30	5537.999999999501
31	8282.999999997028
32	12237.000000004191
33	17402.000000008724
34	23182.16666665462
35	28704.499999968273
36	34021.33333329197
37	40566.666666672674
38	48481.166666729485
39	56490.66666678701
40	65981.33333352208
41	75900.16666671755
42	85514.66666657971
43	95919.66666643161
44	106773.99999961046
45	114137.49999950797
46	120014.33333275779
47	125435.83333268073
48	124791.49999935938
49	124567.16666603036
50	127331.9999993245
51	128224.99999931292
52	127672.66666599059
53	124864.66666602786
54	113349.99999952245
55	95792.16666643987
56	76329.00000004897
57	58895.500000135755
58	45703.00000003977
59	35361.33333329663
60	26476.499999979602
61	19621.833333336643
62	14401.000000007225
63	10238.00000000012
64	7129.999999998099
65	4875.833333333443
66	3392.833333333732
67	2317.166666666587
68	1615.499999999918
69	1188.5000000000143
70	888.5000000000285
71	688.0000000000063
72	455.83333333332536
73	314.83333333333326
74	222.33333333333516
75	142.83333333333292
76	86.66666666666654
77	42.16666666666668
78	20.666666666666664
79	11.333333333333336
80	6.499999999999998
81	3.1666666666666665
82	1.3333333333333333
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07188028633884744
2	3.212766075931799E-4
3	2.570212860745439E-4
4	2.356028455683319E-4
5	2.141844050621199E-4
6	6.425532151863598E-5
7	8.567376202484796E-5
8	1.0709220253105995E-4
9	6.425532151863598E-5
10-14	8.995745012609037E-5
15-19	9.424113822733276E-5
20-24	5.91148957971451E-4
25-29	0.0013836312567012947
30-34	1.0709220253105995E-4
35-39	1.0709220253105995E-4
40-44	8.567376202484796E-5
45-49	8.310354916410251E-4
50-54	0.0014907234592323544
55-59	0.002176113555431138
60-64	0.0019961986551789578
65-69	0.004266553348837429
70-74	0.011124737998926508
75-79	0.012208559725366521
80-84	0.003495538754408459
85-89	7.111109587981882E-4
90-94	0.0013065807287824568
95-99	2.870271319036612E-4
100-104	0.0028746764864358756
105-109	5.484352051012701E-4
110-114	6.599548402330754E-4
115-119	9.901953513971828E-4
120-124	0.0024230810088302648
125-129	0.023542302143301698
130-134	0.018404482870943907
135-139	0.009338235151267105
140-144	0.007714945083363068
145-149	0.007037135134796754
150-154	0.008431610743314052
155-159	0.011345766330315926
160-164	0.013180615489412056
165-169	0.010922714670198777
170-174	0.008708021089039711
175-179	0.011484243984231356
180-184	0.005530814646685213
185-189	0.006369104961554355
190-194	0.005290831843586252
195-199	0.004320910384707503
200-204	0.002966692647571228
205-209	2.685396062568342E-4
210-214	5.991517053854708E-4
215-219	5.188360371303484E-4
220-224	7.593308039570703E-4
225-229	4.168031992749398E-4
230-234	2.07437949221535E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	45.0
80-84	52.0
85-89	68.0
90-94	108.0
95-99	146.0
100-104	340.0
105-109	750.0
110-114	1047.0
115-119	1591.0
120-124	4824.0
125-129	27336.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	2.0
160-164	9.0
165-169	25.0
170-174	59.0
175-179	126.0
180-184	342.0
185-189	806.0
190-194	1560.0
195-199	2744.0
200-204	6268.0
205-209	8547.0
210-214	15528.0
215-219	25701.0
220-224	38401.0
225-229	60963.0
230-234	98702.0
235-239	141533.0
240-244	239682.0
245-249	664737.0
250-253	3326831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.024987858276205
#Duplication Level	Percentage of total
1	18.013276519576692
2	5.8830959100199856
3	3.6647733032915615
4	2.8052048716409437
5	2.345485976503818
6	2.021229512136704
7	1.9079932114134481
8	1.728968318118814
9	1.5894727024552309
>10	28.603700009222504
>50	11.166953776858014
>100	16.139128124047623
>500	2.381648328455164
>1k	1.7490694362595303
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	2.141844050621199E-5
2	0.0	0.0	0.0	0.0	0.0	2.141844050621199E-5
3	0.0	0.0	0.0	0.0	0.0	2.141844050621199E-5
4	0.0	0.0	0.0	0.0	0.0	2.141844050621199E-5
5	0.0	0.0	0.0	2.141844050621199E-5	2.141844050621199E-5	2.141844050621199E-5
6	0.0	0.0	0.0	6.425532151863598E-5	2.141844050621199E-5	4.283688101242398E-5
7	0.0	0.0	0.0	6.425532151863598E-5	2.141844050621199E-5	4.283688101242398E-5
8	0.0	0.0	0.0	8.567376202484796E-5	2.141844050621199E-5	4.283688101242398E-5
9	0.0	0.0	0.0	1.2851064303727195E-4	2.141844050621199E-5	4.283688101242398E-5
10-14	0.0	0.0	2.141844050621199E-5	2.4417022177081665E-4	9.852482632857515E-5	6.425532151863598E-5
15-19	0.0	2.141844050621199E-5	1.1137589063230235E-4	3.76964552909331E-4	1.8848227645466552E-4	6.425532151863598E-5
20-24	0.0	2.141844050621199E-5	1.7991490025218073E-4	0.0014436028901186882	2.827234146819983E-4	8.567376202484796E-5
25-29	0.0	2.141844050621199E-5	2.099007169608775E-4	0.04160317883926617	3.683971767068462E-4	1.4564539544224153E-4
30-34	0.0	2.141844050621199E-5	2.9129079088448305E-4	0.5798485887603735	5.140425721490876E-4	1.7991490025218073E-4
35-39	0.0	2.141844050621199E-5	3.598298005043614E-4	1.874499076222661	5.825815817689661E-4	1.9276596455590793E-4
40-44	0.0	2.141844050621199E-5	3.7268086480808866E-4	4.361449891344252	6.254184627813901E-4	1.9276596455590793E-4
45-49	0.0	2.141844050621199E-5	3.940993053143006E-4	7.104389623707985	6.768227199962989E-4	1.9704965265715033E-4
50-54	0.0	2.141844050621199E-5	4.1123405771927024E-4	10.021902497261653	7.924822987298435E-4	2.141844050621199E-4
55-59	0.0	2.141844050621199E-5	4.583546268329366E-4	12.933268278390035	8.995745012609034E-4	2.141844050621199E-4
60-64	0.0	2.141844050621199E-5	4.712056911366638E-4	15.964564475288904	0.0012122837326515987	2.141844050621199E-4
65-69	0.0	2.141844050621199E-5	4.926241316428757E-4	18.953482145802177	0.0014093333853087491	2.141844050621199E-4
70-74	0.0	2.141844050621199E-5	5.054751959466029E-4	21.956167589872845	0.001495007147333597	2.227517812646047E-4
75-79	0.0	2.141844050621199E-5	5.568794531615117E-4	24.922754394314346	0.0016620709832820505	2.356028455683319E-4
80-84	0.0	2.141844050621199E-5	5.740142055664813E-4	27.86104743884714	0.0018591206359392007	2.356028455683319E-4
85-89	0.0	2.141844050621199E-5	5.997163341739357E-4	30.77656411374563	0.0021032908577100174	2.741560384795135E-4
90-94	0.0	2.141844050621199E-5	5.997163341739357E-4	33.6175746014992	0.004630666837443032	2.9985816708696787E-4
95-99	0.0	2.141844050621199E-5	5.997163341739357E-4	36.408290307256095	0.06191214412725639	3.212766075931799E-4
100-104	0.0	2.141844050621199E-5	6.254184627813901E-4	39.11678061990963	0.2233557812868799	3.212766075931799E-4
105-109	0.0	2.141844050621199E-5	7.068085367049956E-4	41.78146165435178	0.5317727572001301	3.7696455290933104E-4
110-114	0.0	2.141844050621199E-5	7.624964820211468E-4	44.33744838691299	0.839003151509336	4.1123405771927024E-4
115-119	8.567376202484796E-6	2.141844050621199E-5	7.710638582236317E-4	45.847444158912836	1.062020521436218	4.283688101242398E-4
120-124	3.4269504809939184E-5	2.141844050621199E-5	7.710638582236317E-4	45.88759516748578	1.0797078696062479	4.497872506304517E-4
125-129	4.283688101242398E-5	2.141844050621199E-5	7.710638582236317E-4	45.88807065686502	1.0797849759920701	4.626383149341789E-4
130-134	4.283688101242398E-5	2.141844050621199E-5	7.710638582236317E-4	45.88889740866856	1.0798235291849811	4.797730673391486E-4
135-139	4.283688101242398E-5	2.141844050621199E-5	7.881986106286012E-4	45.89156186266753	1.079909202947006	5.097588840478455E-4
140-144	4.283688101242398E-5	2.141844050621199E-5	8.567376202484796E-4	45.89607686992623	1.0799863093328284	5.525957650602693E-4
145-149	4.283688101242398E-5	2.141844050621199E-5	9.381276941720851E-4	45.901362941043175	1.0800934015353594	5.568794531615117E-4
150-154	4.283688101242398E-5	2.141844050621199E-5	9.766808870832666E-4	45.90810546611453	1.0802047774259917	5.740142055664814E-4
155-159	4.283688101242398E-5	2.141844050621199E-5	0.001019517768095691	45.91758955157068	1.080350422821434	5.911489579714509E-4
160-164	4.283688101242398E-5	2.141844050621199E-5	0.001109475218221781	45.93092467262985	1.0804360965834587	6.297021508826324E-4
165-169	5.140425721490878E-5	2.141844050621199E-5	0.001148028411132963	45.94774243211533	1.0805003519049774	6.982411605025109E-4
170-174	6.425532151863598E-5	2.141844050621199E-5	0.0011565957873354476	45.967785808741034	1.0805560398502936	7.453617296161773E-4
175-179	6.425532151863598E-5	2.141844050621199E-5	0.0011822979159429017	45.99474734165025	1.0806759831171286	8.224681154385405E-4
180-184	6.425532151863598E-5	2.141844050621199E-5	0.0012422695493602954	46.03154422243993	1.0807702242553558	8.438865559447523E-4
185-189	6.425532151863598E-5	2.141844050621199E-5	0.0012422695493602954	46.066961755661005	1.0809244370270004	9.252766298683578E-4
190-194	6.425532151863598E-5	2.141844050621199E-5	0.0012636879898665074	46.102319317248664	1.0813313873966186	9.766808870832666E-4
195-199	6.425532151863598E-5	2.141844050621199E-5	0.0012851064303727195	46.137831091607964	1.0824794158077515	0.0010280851442981756
200-204	6.425532151863598E-5	2.141844050621199E-5	0.0012979574946764467	46.1745465823237	1.0928031041317456	0.0010709220253105995
205-209	6.425532151863598E-5	2.141844050621199E-5	0.0013622128161950825	46.21209310853109	1.1311635310783712	0.0010709220253105995
210-214	6.425532151863598E-5	2.141844050621199E-5	0.0013836312567012947	46.24604562042154	1.191949065235001	0.001075205713411842
215-219	6.425532151863598E-5	2.141844050621199E-5	0.0014007660091062643	46.27959974931857	1.267427649578892	0.0011094752182217809
220-224	6.425532151863598E-5	2.570212860745439E-5	0.0014136170734099915	46.3065912680445	1.3896669732359452	0.001152312099234205
225-229	6.425532151863598E-5	4.283688101242398E-5	0.0014136170734099915	46.33878318412534	1.5371243687450122	0.0012165674207528412
230-234	6.425532151863598E-5	4.283688101242398E-5	0.0014350355139162033	46.37584565357729	1.657663068225872	0.0012208511088540835
235-239	6.425532151863598E-5	4.283688101242398E-5	0.0014350355139162033	46.41652355578669	1.7276970849930842	0.0012208511088540835
240-241	6.425532151863598E-5	4.283688101242398E-5	0.0014350355139162033	46.47769462187242	1.7304707730386384	0.0012208511088540835
>>END_MODULE
