##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665020
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4328372
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.4916592196789	30.0	18.0	32.0	18.0	33.0
2	27.11993816612805	29.0	25.0	31.0	18.0	33.0
3	26.445114005912615	29.0	18.0	31.0	18.0	33.0
4	30.0975574650238	31.0	29.0	33.0	27.0	33.0
5	31.62433727969777	33.0	32.0	33.0	29.0	33.0
6	35.58920443991413	37.0	36.0	38.0	30.0	38.0
7	35.93494759692559	38.0	36.0	38.0	31.0	38.0
8	36.50996078895252	38.0	37.0	38.0	34.0	38.0
9	36.73762352219264	38.0	38.0	38.0	34.0	38.0
10-14	36.80094335699427	38.0	38.0	38.0	34.6	38.0
15-19	36.74293581974932	38.0	38.0	38.0	34.0	38.0
20-24	36.74262863727979	38.0	38.0	38.0	34.2	38.0
25-29	36.682913021339196	38.0	38.0	38.0	34.0	38.0
30-34	36.65330586188063	38.0	38.0	38.0	34.0	38.0
35-39	36.586418958444426	38.0	38.0	38.0	34.0	38.0
40-44	36.61026986589878	38.0	38.0	38.0	34.0	38.0
45-49	36.559934451105406	38.0	38.0	38.0	34.0	38.0
50-54	36.4513980776144	38.0	38.0	38.0	33.8	38.0
55-59	36.34375044473997	38.0	37.2	38.0	33.2	38.0
60-64	36.323750685015064	38.0	37.0	38.0	33.0	38.0
65-69	36.26008989061014	38.0	37.0	38.0	33.0	38.0
70-74	36.16512365388188	38.0	37.0	38.0	33.0	38.0
75-79	36.05230423157471	38.0	37.0	38.0	32.6	38.0
80-84	35.91889725591484	38.0	37.0	38.0	31.0	38.0
85-89	35.78318557637943	38.0	37.0	38.0	31.0	38.0
90-94	35.68469015391073	38.0	36.6	38.0	31.0	38.0
95-99	35.559908044153815	38.0	36.0	38.0	29.0	38.0
100-104	35.433099096767855	38.0	36.0	38.0	29.0	38.0
105-109	35.243530263485184	38.0	35.0	38.0	28.0	38.0
110-114	35.124395278997085	38.0	35.0	38.0	28.0	38.0
115-119	34.962717643397085	38.0	35.0	38.0	27.6	38.0
120-124	35.10486205011749	38.0	35.0	38.0	28.8	38.0
125-129	33.2162116482937	34.4	32.8	35.6	28.8	35.6
130-134	34.36588277872455	36.0	35.4	36.8	28.6	37.6
135-139	35.56357603518036	38.0	37.0	38.0	29.0	38.0
140-144	35.458481332314676	38.0	36.2	38.0	29.0	38.0
145-149	35.43070072622284	38.0	36.0	38.0	28.0	38.0
150-154	35.40550437890478	38.0	36.0	38.0	28.0	38.0
155-159	35.39624732681616	38.0	36.0	38.0	28.0	38.0
160-164	35.358835142652836	38.0	36.0	38.0	28.0	38.0
165-169	35.32158504758778	38.0	36.0	38.0	28.0	38.0
170-174	35.27596778502585	38.0	36.0	38.0	28.0	38.0
175-179	35.214077269095085	38.0	36.0	38.0	27.6	38.0
180-184	35.08029422098286	38.0	36.0	38.0	27.0	38.0
185-189	34.96879920798894	38.0	36.0	38.0	27.0	38.0
190-194	34.85744570437219	38.0	35.2	38.0	26.6	38.0
195-199	34.70571084149301	38.0	35.0	38.0	25.0	38.0
200-204	34.508923779917765	38.0	35.0	38.0	24.6	38.0
205-209	34.32904175039374	38.0	34.2	38.0	23.2	38.0
210-214	34.11901750314759	38.0	34.0	38.0	23.0	38.0
215-219	33.86232823961659	38.0	34.0	38.0	21.0	38.0
220-224	33.637625720877935	38.0	34.0	38.0	17.4	38.0
225-229	33.34603058493479	38.0	33.0	38.0	15.0	38.0
230-234	33.15557362525286	38.0	33.0	38.0	15.0	38.0
235-239	32.975849334280724	38.0	31.6	38.0	15.0	38.0
240-244	33.026665483838535	38.0	32.0	38.0	15.6	38.0
245-249	33.49727197985571	38.0	32.6	38.0	21.6	38.0
250-252	35.273411121284965	37.333333333333336	33.666666666666664	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.0
16	9.0
17	21.0
18	49.0
19	109.0
20	508.0
21	2065.0
22	6099.0
23	12967.0
24	22926.0
25	35021.0
26	48404.0
27	64384.0
28	81848.0
29	101454.0
30	122453.0
31	149252.0
32	184280.0
33	238787.0
34	332784.0
35	512067.0
36	913319.0
37	1499563.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.422855114999194	17.67962519636617	12.748571540863237	22.148948147771396
2	17.145323267636456	19.561617276181025	37.01310703073956	26.27995242544296
3	20.61131152032892	26.686444723020188	29.221812573151162	23.480431183499732
4	13.822167533891541	18.996568219970307	40.05698686294089	27.12427738319726
5	14.772110306753172	36.86575950370186	34.28210553358935	14.08002465595562
6	27.108195463881323	39.435792226634966	20.04073126835275	13.415281041130958
7	23.861158787524815	31.21397459412541	26.559912059253726	18.36495455909605
8	23.645060596756238	34.80825447564867	21.657636702248215	19.88904822534688
9	23.653032515003915	16.957927127780867	21.461954162432008	37.92708619478321
10-14	24.181716249686776	23.030867368969503	28.492659893093247	24.294756488250474
15-19	23.84673566045714	27.44853940635669	25.31305201204832	23.391672921137847
20-24	23.610403951364535	26.351172611406138	26.391164776545917	23.647258660683413
25-29	23.75343394835316	26.399883964394	26.224485924883766	23.62219616236908
30-34	23.65069479760549	26.31798162097293	26.349166582031238	23.682156999390347
35-39	23.540871861895322	25.871047500802263	26.624934241974284	23.963146395328124
40-44	23.205347012019864	25.628537013759082	27.043171552294716	24.122944421926338
45-49	22.610497649859195	25.583307249408804	27.575989703572944	24.230205397159057
50-54	22.170341637271477	25.83766184528403	27.36131684291613	24.63067967452837
55-59	21.940711328028325	25.481178790538667	27.32138015240763	25.256729729025373
60-64	22.010502704691127	25.513896396978154	26.63331650299404	25.84228439533668
65-69	21.970602971734575	25.75950748372386	25.99006027023389	26.27982927430768
70-74	22.365159613231693	25.98028745603782	25.34898714959069	26.3055657811398
75-79	22.685184115452728	26.110783054119295	24.96936346150416	26.234669368923814
80-84	22.806111358791966	26.084551035934428	24.888765418133342	26.220572187140263
85-89	22.83089645175989	25.62928584891872	25.184973171791242	26.354844527530148
90-94	22.881299226679833	25.13563185841755	25.30671626079542	26.6763526541072
95-99	22.702249732113135	24.821217748907426	25.74787894785388	26.728653571125555
100-104	22.616650708610706	25.06960679405567	25.830149347623767	26.483593149709854
105-109	22.227349147229006	26.245919041134474	25.506028730726925	26.020703080909595
110-114	21.952454867669143	27.413385182223116	25.046290844512647	25.58786910559509
115-119	21.813924637456466	27.757874992790367	25.030997389545018	25.397202980208156
120-124	21.821431997333526	28.072256536469652	24.88628590183955	25.22002556435727
125-129	23.304985529485904	25.30245974846762	27.147979767214498	24.244574954831982
130-134	21.40020075867759	31.109250204975204	27.629259759958895	19.861289276388305
135-139	24.38340674693803	22.723067334212505	27.67536184077794	25.21816407807153
140-144	23.397309174673804	26.253068335336522	25.72494203449351	24.624680455496165
145-149	23.374234342342103	26.295418140390403	26.640521854912503	23.68982566235499
150-154	23.404591514971862	26.126846844035757	26.586423668949415	23.882137972042965
155-159	23.24752992739545	26.06374632859675	26.604225550473092	24.08449819353471
160-164	23.19029498148385	25.833849154293542	26.815894870038658	24.159960994183955
165-169	23.045796938914958	25.887817793870337	26.63269733039056	24.433687936824146
170-174	22.87148268408319	25.877701473586768	26.846677919749716	24.404137922580325
175-179	22.97817743631893	25.58321898486421	26.70946666016564	24.729136918651225
180-184	22.99593219227676	25.256325844767453	26.878810014512528	24.86893194844326
185-189	22.67875485167673	24.906835127957756	27.38446039518226	25.029949625183257
190-194	22.56558553260242	25.067909415710055	27.224356803507494	25.14214824818003
195-199	22.442607307343522	24.929365270877074	27.477797326755756	25.15023009502365
200-204	22.86998287826628	24.530598941586696	27.1263540596287	25.473064120518323
205-209	22.951522693755656	24.18913610291417	27.271865157205706	25.58747604612447
210-214	23.00540187053148	24.432200434881597	26.960301613269095	25.602096081317825
215-219	22.767173580320907	24.549511257536896	26.725807296123477	25.95750786601872
220-224	23.148250162916494	24.234358542172593	27.234560539278007	25.382830755632906
225-229	22.79184648931664	24.794631381201903	26.88824744359202	25.52527468588944
230-234	23.09636000738762	25.029549515957704	26.72795142929734	25.146139047357323
235-239	22.999166645010007	24.76344340830911	27.152589827901334	25.084800118779548
240-244	23.049406156089713	24.738369523348833	26.772814896014275	25.439409424547176
245-249	22.60393312797411	25.410738154827435	26.912642834051887	25.072685883146573
250-252	21.820837145625173	25.483715084639407	26.774661640922158	25.920786128813262
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.8333333333333333
10	0.8333333333333333
11	0.5
12	1.1666666666666665
13	1.0
14	1.9999999999999998
15	3.666666666666667
16	4.666666666666666
17	10.0
18	16.333333333333343
19	24.833333333333314
20	43.50000000000002
21	70.66666666666677
22	117.66666666666609
23	194.1666666666678
24	295.16666666666754
25	502.1666666666559
26	828.0000000000231
27	1249.8333333333317
28	1912.8333333331805
29	2899.8333333335268
30	4475.666666667101
31	6618.833333331833
32	9382.333333332406
33	12949.333333338845
34	17802.333333340513
35	23706.833333319144
36	31213.333333292023
37	39388.499999998756
38	47995.00000006097
39	57133.16666679337
40	65934.83333352361
41	75049.50000005936
42	85259.49999991205
43	95930.16666642563
44	106435.8333329414
45	116863.16666612541
46	125108.49999934113
47	131879.66666591103
48	136529.66666602614
49	136780.83333269885
50	131526.9999992535
51	120746.8333327433
52	107543.66666626596
53	93190.83333313966
54	78285.50000001979
55	64842.16666684537
56	52582.33333342459
57	41195.666666676574
58	32308.499999955468
59	25600.333333312796
60	20641.666666664012
61	16678.500000011674
62	13502.833333339591
63	11015.83333333515
64	9072.333333331742
65	7278.333333331268
66	5687.333333332702
67	4327.83333333392
68	3209.5000000003224
69	2320.8333333332585
70	1667.3333333332378
71	1129.1666666666922
72	761.3333333333487
73	488.33333333332337
74	334.99999999999875
75	222.8333333333351
76	129.83333333333263
77	77.50000000000003
78	45.50000000000003
79	31.333333333333293
80	17.333333333333343
81	6.999999999999998
82	3.0
83	2.333333333333333
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06716151014746423
2	1.8482699730984305E-4
3	1.386202479823823E-4
4	1.386202479823823E-4
5	1.386202479823823E-4
6	4.620674932746076E-5
7	6.931012399119115E-5
8	1.1551687331865192E-4
9	2.310337466373038E-5
10-14	4.1586074394714684E-5
15-19	4.1586074394714684E-5
20-24	5.591016668622753E-4
25-29	0.0010488932097333593
30-34	8.317214878942937E-5
35-39	6.931012399119115E-5
40-44	7.393079892393721E-5
45-49	8.224801380288016E-4
50-54	0.0012984096561016473
55-59	0.0019591661714843363
60-64	0.0017882011989727316
65-69	0.0038305395192464974
70-74	0.01034107049948572
75-79	0.011755063294824102
80-84	0.0030219848008026834
85-89	6.838874138330739E-4
90-94	0.0012569044255419984
95-99	2.3567703982983564E-4
100-104	0.002745098474168901
105-109	4.8531710087833615E-4
110-114	5.039503232587036E-4
115-119	9.527961212872461E-4
120-124	0.002100780939864405
125-129	0.02308031583482676
130-134	0.017842753940623048
135-139	0.009081557821456851
140-144	0.00761439408343008
145-149	0.006690452362330942
150-154	0.008440835592580086
155-159	0.01128695735665866
160-164	0.013218438007777568
165-169	0.01068808666951837
170-174	0.008380603263216548
175-179	0.01135693768672087
180-184	0.005107658529996373
185-189	0.005981439796645908
190-194	0.004970377479266094
195-199	0.0039131540067769135
200-204	0.0027908265716644494
205-209	2.1425811358213856E-4
210-214	6.675789935018888E-4
215-219	5.671873227539616E-4
220-224	8.398781308521636E-4
225-229	4.766016245252333E-4
230-234	3.0038768422122606E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	69.0
80-84	64.0
85-89	105.0
90-94	114.0
95-99	176.0
100-104	404.0
105-109	1054.0
110-114	1573.0
115-119	1734.0
120-124	2928.0
125-129	12520.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	2.0
155-159	6.0
160-164	13.0
165-169	28.0
170-174	44.0
175-179	139.0
180-184	433.0
185-189	893.0
190-194	1644.0
195-199	2772.0
200-204	5026.0
205-209	7627.0
210-214	13855.0
215-219	23358.0
220-224	35318.0
225-229	55709.0
230-234	88438.0
235-239	128813.0
240-244	219930.0
245-249	613619.0
250-253	3109964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.73567078344531
#Duplication Level	Percentage of total
1	35.26665327470539
2	10.558493731178718
3	5.8055090136086065
4	4.236347546479216
5	3.3162646690967703
6	2.769343521670397
7	2.34652399996311
8	1.972989525861905
9	1.8074794063611095
>10	21.88067779741759
>50	5.48077196110957
>100	4.4572111098735725
>500	0.10173444267398042
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.310337466373038E-5	0.0	4.620674932746076E-5
7	0.0	0.0	0.0	2.310337466373038E-5	2.310337466373038E-5	4.620674932746076E-5
8	0.0	0.0	0.0	2.310337466373038E-5	2.310337466373038E-5	4.620674932746076E-5
9	0.0	0.0	0.0	6.931012399119115E-5	2.310337466373038E-5	4.620674932746076E-5
10-14	0.0	4.6206749327460766E-6	0.0	2.541371213010342E-4	6.931012399119115E-5	4.620674932746076E-5
15-19	0.0	2.310337466373038E-5	0.0	4.80550193005592E-4	2.1255104690631952E-4	4.620674932746076E-5
20-24	0.0	2.310337466373038E-5	0.0	0.0018528906480311767	2.6799914609927243E-4	4.620674932746076E-5
25-29	0.0	2.310337466373038E-5	0.0	0.04510240801853445	3.511712948887018E-4	1.1551687331865192E-4
30-34	0.0	2.310337466373038E-5	0.0	0.5752416844023572	5.082742426020684E-4	1.386202479823823E-4
35-39	0.0	3.2344724529222534E-5	0.0	1.7971375842926622	5.914463913914978E-4	1.6172362264611268E-4
40-44	0.0	4.620674932746076E-5	0.0	4.126068646595071	6.376531407189586E-4	1.9868902210808128E-4
45-49	0.0	4.620674932746076E-5	9.241349865492153E-6	6.682078157792352	7.901354134995792E-4	2.0793037197357344E-4
50-54	0.0	4.620674932746076E-5	2.310337466373038E-5	9.401091218592118	0.0011921341326484875	2.2179239677181167E-4
55-59	0.0	4.620674932746076E-5	2.310337466373038E-5	12.122835098277136	0.0017096497251160483	2.541371213010342E-4
60-64	0.0	6.0068774125699E-5	2.310337466373038E-5	14.88674263672346	0.0025090264884811194	3.00343870628495E-4
65-69	0.0	6.931012399119115E-5	2.310337466373038E-5	17.59701338055047	0.0029572319569574888	3.00343870628495E-4
70-74	0.0	6.931012399119115E-5	4.620674932746076E-5	20.34388911119469	0.003128196929469094	3.00343870628495E-4
75-79	0.0	6.931012399119115E-5	4.620674932746076E-5	23.092811800834124	0.003336127301442667	3.142058954267332E-4
80-84	0.0	6.931012399119115E-5	4.620674932746076E-5	25.825381921886567	0.0034793682243577954	3.2344724529222537E-4
85-89	0.0	6.931012399119115E-5	4.620674932746076E-5	28.494302245740432	0.003719643320860591	3.6503331968694E-4
90-94	0.0	6.931012399119115E-5	4.620674932746076E-5	31.141921258154337	0.0065336343549029525	4.389641186108772E-4
95-99	0.0	6.931012399119115E-5	4.620674932746076E-5	33.76020360542024	0.06340490142714166	4.6206749327460766E-4
100-104	0.0	6.931012399119115E-5	4.620674932746076E-5	36.30648197520915	0.2129900110249304	4.897915428710841E-4
105-109	0.0	6.931012399119115E-5	4.620674932746076E-5	38.80687242224097	0.5045083925318804	5.221362674003066E-4
110-114	0.0	6.931012399119115E-5	4.620674932746076E-5	41.20530767688175	0.7932959551535774	5.544809919295292E-4
115-119	0.0	6.931012399119115E-5	4.620674932746076E-5	42.619271171701506	1.0055096927898064	5.544809919295292E-4
120-124	1.8482699730984306E-5	6.931012399119115E-5	4.620674932746076E-5	42.652470721093295	1.024791769284156	5.868257164587517E-4
125-129	2.310337466373038E-5	6.931012399119115E-5	4.620674932746076E-5	42.65290044386204	1.0248194933337522	6.284117908534664E-4
130-134	2.310337466373038E-5	6.931012399119115E-5	4.620674932746076E-5	42.654000164496026	1.0248657000830796	6.468944905844507E-4
135-139	2.310337466373038E-5	6.931012399119115E-5	4.620674932746076E-5	42.65686960362926	1.0249673549316	6.931012399119115E-4
140-144	2.310337466373038E-5	6.931012399119115E-5	4.620674932746076E-5	42.660797177322095	1.025078251129986	7.393079892393722E-4
145-149	4.1586074394714684E-5	6.931012399119115E-5	4.620674932746076E-5	42.66643902141498	1.0251244578793135	7.393079892393722E-4
150-154	4.620674932746076E-5	7.85514738566833E-5	4.620674932746076E-5	42.673074310618404	1.0252168713779686	7.439286641721183E-4
155-159	4.620674932746076E-5	9.241349865492152E-5	4.620674932746076E-5	42.68210773011192	1.0252676988022287	7.624113639031026E-4
160-164	4.620674932746076E-5	9.241349865492152E-5	6.931012399119115E-5	42.69452810433114	1.0253416296011528	7.624113639031026E-4
165-169	4.620674932746076E-5	9.241349865492152E-5	6.931012399119115E-5	42.71034467462594	1.0254155604000765	8.271008129615478E-4
170-174	4.620674932746076E-5	9.241349865492152E-5	6.931012399119115E-5	42.729608268420556	1.0255449392981935	8.825489121545005E-4
175-179	4.620674932746076E-5	1.1089619838590584E-4	6.931012399119115E-5	42.754546051032584	1.0256234907720498	9.010316118854848E-4
180-184	4.620674932746076E-5	1.1551687331865192E-4	6.931012399119115E-5	42.78728353293109	1.0257990764194944	9.241349865492151E-4
185-189	4.620674932746076E-5	1.1551687331865192E-4	6.931012399119115E-5	42.82052004772233	1.026020868816266	9.472383612129456E-4
190-194	4.620674932746076E-5	1.2475822318414406E-4	7.85514738566833E-5	42.854468146453215	1.0263304540367604	9.472383612129456E-4
195-199	4.620674932746076E-5	1.386202479823823E-4	9.241349865492152E-5	42.88844858990863	1.0275734155936689	9.657210609439299E-4
200-204	4.620674932746076E-5	1.6172362264611268E-4	9.241349865492152E-5	42.922636963736025	1.0379791755422132	9.934451105404064E-4
205-209	4.620674932746076E-5	1.6172362264611268E-4	9.241349865492152E-5	42.95857195268799	1.0732718906785275	0.0010165484852041368
210-214	4.620674932746076E-5	1.8020632237709697E-4	9.241349865492152E-5	42.99193784637734	1.1285444042240362	0.0010442725348006133
215-219	4.620674932746076E-5	1.8482699730984305E-4	9.241349865492152E-5	43.02421788145751	1.1941117815197029	0.0011182033337245503
220-224	4.620674932746076E-5	1.8482699730984305E-4	1.20137548251398E-4	43.05073131422161	1.3020784719982479	0.0011320653585227887
225-229	4.620674932746076E-5	1.8482699730984305E-4	1.386202479823823E-4	43.081112251904415	1.4322752295782342	0.0011366860334555348
230-234	4.620674932746076E-5	1.8482699730984305E-4	1.386202479823823E-4	43.11571648647575	1.5428710840935114	0.0011551687331865191
235-239	4.620674932746076E-5	1.8482699730984305E-4	1.5248227278062053E-4	43.15425753608979	1.6091546660037541	0.0011551687331865191
240-241	4.620674932746076E-5	1.8482699730984305E-4	1.6172362264611268E-4	43.213545416151845	1.6119917604124598	0.0011551687331865191
>>END_MODULE
