##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665023
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1486428
Total Bases	369.8 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.924077721894367	28.0	18.0	32.0	18.0	33.0
2	26.562208193064176	28.0	25.0	31.0	18.0	33.0
3	25.743363284329952	28.0	18.0	31.0	18.0	33.0
4	30.25373714703975	31.0	29.0	33.0	27.0	33.0
5	31.834788499678425	33.0	32.0	33.0	30.0	33.0
6	35.91353432524145	37.0	36.0	38.0	31.0	38.0
7	36.09429451006036	38.0	36.0	38.0	33.0	38.0
8	36.655007844308635	38.0	37.0	38.0	34.0	38.0
9	36.84974516088233	38.0	38.0	38.0	35.0	38.0
10-14	36.89902369976885	38.0	38.0	38.0	35.0	38.0
15-19	36.85945367014076	38.0	38.0	38.0	35.0	38.0
20-24	36.85858003213072	38.0	38.0	38.0	35.0	38.0
25-29	36.802631274437786	38.0	38.0	38.0	35.0	38.0
30-34	36.77718207676389	38.0	38.0	38.0	35.0	38.0
35-39	36.71912423608812	38.0	38.0	38.0	34.6	38.0
40-44	36.74340634056947	38.0	38.0	38.0	34.8	38.0
45-49	36.696946774414904	38.0	38.0	38.0	34.0	38.0
50-54	36.59907819282199	38.0	38.0	38.0	34.0	38.0
55-59	36.49828219059383	38.0	38.0	38.0	34.0	38.0
60-64	36.48190292432597	38.0	38.0	38.0	34.0	38.0
65-69	36.42222643814567	38.0	37.8	38.0	34.0	38.0
70-74	36.34972874569102	38.0	37.2	38.0	33.4	38.0
75-79	36.28212001691871	38.0	37.0	38.0	33.0	38.0
80-84	36.19556950293005	38.0	37.0	38.0	33.0	38.0
85-89	36.09211025434902	38.0	37.0	38.0	33.0	38.0
90-94	36.00986275181613	38.0	37.0	38.0	32.0	38.0
95-99	35.92234859142125	38.0	37.0	38.0	31.0	38.0
100-104	35.81224433224686	38.0	37.0	38.0	31.0	38.0
105-109	35.65662136348912	38.0	36.0	38.0	31.0	38.0
110-114	35.54433708641614	38.0	36.0	38.0	29.4	38.0
115-119	35.396445122602394	38.0	35.6	38.0	29.0	38.0
120-124	35.5043366638555	38.0	35.2	38.0	30.6	38.0
125-129	33.59267829796139	34.4	33.4	35.6	31.0	35.6
130-134	34.98262194442475	36.2	35.6	37.0	32.2	37.2
135-139	36.23625999952725	38.0	37.8	38.0	33.0	38.0
140-144	36.155255471248296	38.0	37.0	38.0	32.8	38.0
145-149	36.13644781673471	38.0	37.0	38.0	33.0	38.0
150-154	36.12016681355714	38.0	37.0	38.0	33.0	38.0
155-159	36.1128540141509	38.0	37.0	38.0	33.0	38.0
160-164	36.077113047947655	38.0	37.0	38.0	32.4	38.0
165-169	36.05092961972136	38.0	37.0	38.0	31.6	38.0
170-174	36.01960986028434	38.0	37.0	38.0	31.0	38.0
175-179	35.98193872957721	38.0	37.0	38.0	31.0	38.0
180-184	35.88321893090697	38.0	37.0	38.0	31.0	38.0
185-189	35.81588527032981	38.0	37.0	38.0	30.8	38.0
190-194	35.741690279228706	38.0	37.0	38.0	29.2	38.0
195-199	35.640958816022824	38.0	37.0	38.0	29.0	38.0
200-204	35.50347188223118	38.0	37.0	38.0	29.0	38.0
205-209	35.37822880982215	38.0	36.2	38.0	28.6	38.0
210-214	35.219778297009405	38.0	36.0	38.0	27.8	38.0
215-219	35.02479435210806	38.0	36.0	38.0	26.2	38.0
220-224	34.851163652704535	38.0	35.4	38.0	25.2	38.0
225-229	34.6179930656677	38.0	35.0	38.0	24.4	38.0
230-234	34.463089342834465	38.0	35.0	38.0	23.2	38.0
235-239	34.319877360944005	38.0	34.0	38.0	23.0	38.0
240-244	34.32288101864528	38.0	34.0	38.0	23.0	38.0
245-249	34.63393148599519	38.0	34.2	38.0	25.0	38.0
250-252	35.86888624337892	37.666666666666664	35.0	38.0	31.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	3.0
16	4.0
17	7.0
18	13.0
19	32.0
20	96.0
21	327.0
22	1070.0
23	2254.0
24	4343.0
25	7082.0
26	10549.0
27	14765.0
28	19554.0
29	25200.0
30	32030.0
31	39882.0
32	50553.0
33	67197.0
34	96022.0
35	152440.0
36	300730.0
37	662274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4558747298762	22.950795392579927	15.403081935870421	20.190247941673455
2	12.775207107518302	22.04125869703571	43.98691895862963	21.196615236816363
3	15.758930643751762	31.044175058714625	34.62760027905844	18.569294018475176
4	10.447603548643828	20.82840260571155	47.98547120040204	20.738522645242586
5	10.290313617823141	40.36861547859397	39.26415491645402	10.076915987128867
6	20.39588893366442	48.291170706667735	21.581550927156194	9.731389432511655
7	18.878962774492123	36.05060995259101	31.008317260114353	14.062110012802512
8	18.48735255750871	41.847329199483056	24.415527974128565	15.249790268879671
9	21.281435280710053	19.212245202758023	27.266391151398622	32.2399283651333
10-14	19.282644796232145	27.27200559085722	33.914162473389204	19.531187139521435
15-19	18.611448796341833	33.027763892878696	30.090949639639213	18.26983767114026
20-24	18.577311547829428	31.638939637232987	31.102563502257368	18.681185312680213
25-29	18.764260862474895	31.362056841890546	31.127962740851988	18.745719554782568
30-34	18.64655311024356	31.39100675647038	31.299821652862867	18.66261848042319
35-39	18.653296617095442	31.23435341720157	31.264075701541444	18.848274264161542
40-44	18.558196071276434	30.553243523327218	31.67214154104326	19.21641886435309
45-49	18.24472231158696	30.55298449736003	31.88691631652958	19.315376874523434
50-54	17.91178123604439	30.66916826548594	31.739492497827314	19.679558000642352
55-59	17.85680262992182	29.92332623969188	31.65176894319812	20.568102187188174
60-64	17.995963401506998	30.05449407965554	31.015971474703985	20.933571044133476
65-69	17.80440848154216	29.913281419876814	30.544644567924887	21.737665530656137
70-74	17.899369109114897	29.57370324498923	30.20946535482701	22.317462291068864
75-79	17.560428484633587	29.147195546408067	30.3608854703178	22.931490498640546
80-84	17.323903579148777	29.103076712961002	30.27622532645209	23.296794381438133
85-89	17.301145614398628	28.462866431103013	30.453253711742214	23.78273424275615
90-94	17.3917349388998	28.093335997615604	30.49190320581782	24.023025857666774
95-99	17.26790393462622	27.514407962558202	30.662569143351103	24.555118959464473
100-104	17.431892801593754	27.42015011157305	30.666789933960487	24.481167152872708
105-109	17.137184306950985	28.268620335007625	30.30078604133991	24.293409316701474
110-114	16.886340209169052	29.161285876310334	29.86934269961016	24.08303121491045
115-119	16.7587095391367	29.29056910943455	29.88069902768314	24.070022323745608
120-124	16.87077120324082	29.585550982589208	29.800963734700915	23.742714079469057
125-129	18.000669243122207	28.822551900425047	32.550416967621345	20.6263618888314
130-134	16.442297261350852	36.51107995200856	32.00953830594963	15.037084480690957
135-139	19.801374868463533	26.697249317496276	33.11235206790861	20.389023746131578
140-144	18.360483765191283	31.8148705128574	30.527881012771424	19.296764709179897
145-149	18.521864484072523	31.483552197903137	31.30874600975597	18.68583730826837
150-154	18.52038364186366	31.170806865266883	31.440388731729236	18.86842076114023
155-159	18.479587935802062	31.102741826326234	31.349583194308046	19.068087043563658
160-164	18.442101639927138	30.846087091375864	31.50267395283014	19.209137315866858
165-169	18.417059047931932	30.80913843989137	31.331896193046177	19.44190631913052
170-174	18.410140300014362	30.737267720676247	31.325182070138798	19.527409909170597
175-179	18.53831471634667	30.332433678546984	31.194416349463943	19.934835255642408
180-184	18.67235914196809	29.92222490140495	31.19945209587512	20.20596386075184
185-189	18.466400316993308	29.61823138430994	31.54291467132952	20.372453627367232
190-194	18.446758440118842	29.57729922412371	31.347101918362675	20.628840417394773
195-199	18.535223945349482	29.263489802358826	31.36444513222368	20.83684112006801
200-204	18.93706890583216	28.759606314204362	30.971425001514508	21.33189977844897
205-209	19.179983317446155	28.163442469212864	31.055563352898275	21.60101086044271
210-214	19.24097979971147	28.209688359508352	30.76199491655548	21.787336924224693
215-219	19.15674155845585	28.443007324430297	30.39442260249658	22.005828514617274
220-224	19.496239427118823	27.929730795227993	30.79383657553578	21.780193202117406
225-229	19.511210322610417	28.28414920508572	30.22561277246425	21.97902769983962
230-234	19.691759742499073	28.32679697514776	30.068868659355097	21.91257462299807
235-239	19.90720683398838	28.006687811049662	30.358376486170403	21.72772886879155
240-244	19.727790319752582	27.89148633842985	30.19337961547952	22.187343726338046
245-249	19.59853601069122	28.436191827301414	30.04086814938527	21.924404012622094
250-252	18.309725423172868	28.973386792805698	31.20024140203077	21.51664638199066
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.3333333333333333
12	6.999999999999997
13	10.5
14	17.666666666666675
15	28.8333333333333
16	49.3333333333334
17	84.83333333333323
18	113.83333333333282
19	179.66666666666734
20	263.8333333333359
21	481.3333333333237
22	899.3333333333647
23	1570.6666666665917
24	2732.5000000001132
25	4632.6666666669535
26	7344.999999997827
27	10778.000000001628
28	14941.833333342483
29	19926.33333333283
30	26036.83333331087
31	31693.99999995717
32	35944.999999973494
33	39297.166666664685
34	41737.6666666827
35	43491.66666669544
36	44622.00000003689
37	44820.1666667052
38	44434.33333336921
39	42556.83333335542
40	39090.999999996624
41	34881.666666632664
42	29920.83333329696
43	25587.833333312738
44	21521.99999999406
45	17841.33333334056
46	14774.833333341952
47	12498.833333337827
48	10361.833333333987
49	8550.999999997402
50	7267.499999998001
51	6377.499999998778
52	5700.16666666605
53	5172.666666666515
54	4943.166666666715
55	4721.500000000245
56	4411.833333333842
57	4067.3333333340397
58	3787.000000000579
59	3472.5000000004356
60	3206.666666666987
61	2929.1666666668666
62	2592.833333333378
63	2265.33333333323
64	1959.9999999998392
65	1714.9999999998931
66	1495.4999999999416
67	1295.333333333321
68	1096.0000000000332
69	859.0000000000268
70	665.3333333333385
71	509.3333333333221
72	387.99999999999574
73	277.00000000000193
74	204.33333333333468
75	141.1666666666662
76	101.16666666666634
77	67.33333333333348
78	42.66666666666668
79	22.33333333333333
80	15.333333333333341
81	10.166666666666666
82	4.5
83	3.0
84	1.9999999999999998
85	1.5
86	0.8333333333333333
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06714082350440116
2	1.3455074850581393E-4
3	6.727537425290697E-5
4	6.727537425290697E-5
5	6.727537425290697E-5
6	6.727537425290697E-5
7	6.727537425290697E-5
8	6.727537425290697E-5
9	6.727537425290697E-5
10-14	8.073044910348836E-5
15-19	6.727537425290697E-5
20-24	5.651131437244186E-4
25-29	0.0011571364371499998
30-34	6.727537425290697E-5
35-39	8.073044910348836E-5
40-44	8.073044910348836E-5
45-49	6.86208817379651E-4
50-54	0.0012513219611040695
55-59	0.0017760698802767442
60-64	0.0018837104790813952
65-69	0.003821241257565116
70-74	0.010010575688832556
75-79	0.010750656879936017
80-84	0.002879436771403264
85-89	5.920452796229856E-4
90-94	0.0010764660963638994
95-99	1.8838739715225538E-4
100-104	0.002476050269203183
105-109	3.4991273714662853E-4
110-114	2.9612767302336125E-4
115-119	6.731679431501594E-4
120-124	0.0010908560540019536
125-129	0.02337350057711764
130-134	0.017856479558585665
135-139	0.007901694812233444
140-144	0.006240312826071541
145-149	0.005659504489445835
150-154	0.0071452932575581055
155-159	0.009954795503241926
160-164	0.012413143452309958
165-169	0.010076437749631588
170-174	0.007861294349958081
175-179	0.010833049337245658
180-184	0.0042414841736582495
185-189	0.00518748362869343
190-194	0.004364054566084621
195-199	0.0032841130826901837
200-204	0.002461016551282852
205-209	1.3534424198900815E-4
210-214	7.995756557130223E-4
215-219	7.607904884886286E-4
220-224	9.821659131953582E-4
225-229	7.553420882516975E-4
230-234	3.890398579671056E-4
235-239	4.246047708025908E-5
240-244	0.0
245-249	1.552151273904876E-5
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	16.0
80-84	27.0
85-89	29.0
90-94	36.0
95-99	52.0
100-104	95.0
105-109	211.0
110-114	299.0
115-119	376.0
120-124	723.0
125-129	3869.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	4.0
160-164	6.0
165-169	6.0
170-174	14.0
175-179	26.0
180-184	92.0
185-189	164.0
190-194	318.0
195-199	594.0
200-204	1179.0
205-209	1566.0
210-214	2731.0
215-219	5052.0
220-224	7879.0
225-229	13445.0
230-234	22414.0
235-239	33047.0
240-244	57288.0
245-249	173046.0
250-253	1161824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.48209288702412
#Duplication Level	Percentage of total
1	23.091347407316807
2	10.462723433503886
3	6.808159927451742
4	5.333495426129337
5	4.385664256656772
6	3.8048836728260813
7	3.367645080002194
8	3.0974428876807147
9	2.690399791843421
>10	32.26184166116634
>50	3.953000724449588
>100	0.7433957309730139
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.727537425290697E-5	1.3455074850581393E-4	0.0
10-14	0.0	0.0	0.0	1.2109567365523254E-4	9.687653892418603E-4	0.0
15-19	0.0	2.6910149701162787E-5	0.0	2.018261227587209E-4	0.002489188847357558	8.073044910348836E-5
20-24	0.0	6.727537425290697E-5	0.0	3.76742095816279E-4	0.004453629775542442	2.018261227587209E-4
25-29	0.0	6.727537425290697E-5	0.0	0.012661225434397091	0.005260934266577325	2.018261227587209E-4
30-34	0.0	6.727537425290697E-5	0.0	0.2928093388983523	0.006902453398348256	2.018261227587209E-4
35-39	0.0	6.727537425290697E-5	0.0	1.1512296592905948	0.007777033263636046	2.018261227587209E-4
40-44	0.0	6.727537425290697E-5	0.0	2.992193365571693	0.008194140584004068	2.018261227587209E-4
45-49	0.0	6.727537425290697E-5	0.0	5.0428275032494	0.008745798652877907	2.018261227587209E-4
50-54	0.0	6.727537425290697E-5	0.0	7.327849044824236	0.01013167136248779	2.018261227587209E-4
55-59	0.0	6.727537425290697E-5	0.0	9.610542858449922	0.011827010793661046	2.018261227587209E-4
60-64	0.0	6.727537425290697E-5	0.0	12.043112750836233	0.014006732919455232	2.152811976093023E-4
65-69	0.0	6.727537425290697E-5	0.0	14.417704725691388	0.015513701302720345	2.6910149701162787E-4
70-74	0.0	6.727537425290697E-5	0.0	16.918357296821643	0.01738395670695116	2.6910149701162787E-4
75-79	0.0	6.727537425290697E-5	0.0	19.41629194283208	0.019052385988423255	2.6910149701162787E-4
80-84	0.0	6.727537425290697E-5	0.0	22.011035852392446	0.019859690479458136	2.960116467127907E-4
85-89	0.0	6.727537425290697E-5	0.0	24.543388579870673	0.021124467515412786	4.036522455174418E-4
90-94	0.0	6.727537425290697E-5	0.0	27.095654818127752	0.02319654904240232	4.036522455174418E-4
95-99	0.0	6.727537425290697E-5	0.0	29.63827376771697	0.05219223534540522	4.574725449197673E-4
100-104	0.0	6.727537425290697E-5	0.0	32.18202294359364	0.15759929172485984	4.7092761977034876E-4
105-109	0.0	6.727537425290697E-5	0.0	34.68443812952931	0.3937224002777127	4.7092761977034876E-4
110-114	0.0	6.727537425290697E-5	0.0	37.11503012591258	0.6369094231271208	5.247479191726744E-4
115-119	0.0	6.727537425290697E-5	0.0	38.60738629788998	0.8310661532210103	5.382029940232557E-4
120-124	5.3820299402325574E-5	6.727537425290697E-5	0.0	38.66062802907373	0.8531593861256649	5.651131437244184E-4
125-129	6.727537425290697E-5	6.727537425290697E-5	0.0	38.66084331027133	0.8532132064250673	6.054783682761627E-4
130-134	6.727537425290697E-5	6.727537425290697E-5	0.0	38.66146224371446	0.8534823079220789	6.054783682761627E-4
135-139	6.727537425290697E-5	6.727537425290697E-5	0.0	38.66335940926839	0.8540877862903551	6.054783682761627E-4
140-144	6.727537425290697E-5	6.727537425290697E-5	0.0	38.665888963340294	0.8551238270538499	6.054783682761627E-4
145-149	6.727537425290697E-5	6.727537425290697E-5	0.0	38.67058478446315	0.8566846157365173	6.054783682761627E-4
150-154	6.727537425290697E-5	6.727537425290697E-5	0.0	38.676182095601	0.8577341115748626	6.054783682761627E-4
155-159	6.727537425290697E-5	6.727537425290697E-5	0.0	38.6842147752868	0.8585952363652998	6.054783682761627E-4
160-164	6.727537425290697E-5	6.727537425290697E-5	0.0	38.693821698730105	0.8593083553323806	6.054783682761627E-4
165-169	6.727537425290697E-5	6.727537425290697E-5	0.0	38.705877445796226	0.8596312771287946	6.592986676784882E-4
170-174	6.727537425290697E-5	6.727537425290697E-5	0.0	38.72242718786244	0.8602098453473698	7.400291167819767E-4
175-179	6.727537425290697E-5	6.727537425290697E-5	0.0	38.74287890163533	0.8606134975928871	7.669392664831395E-4
180-184	6.727537425290697E-5	6.727537425290697E-5	0.0	38.770152338357455	0.8613131614851174	8.073044910348836E-4
185-189	6.727537425290697E-5	6.727537425290697E-5	0.0	38.7976948765766	0.8619993703024971	8.073044910348836E-4
190-194	6.727537425290697E-5	6.727537425290697E-5	0.0	38.824941403149026	0.8629681356917388	8.073044910348836E-4
195-199	6.727537425290697E-5	6.727537425290697E-5	0.0	38.85366798795501	0.8651613128923836	8.073044910348836E-4
200-204	6.727537425290697E-5	6.727537425290697E-5	0.0	38.881937100216085	0.8697898586409837	8.073044910348836E-4
205-209	6.727537425290697E-5	6.727537425290697E-5	0.0	38.914350375531136	0.8907125000336377	8.073044910348836E-4
210-214	1.2109567365523254E-4	6.727537425290697E-5	0.0	38.94720766831627	0.9305664317410599	8.073044910348836E-4
215-219	1.3455074850581393E-4	6.727537425290697E-5	0.0	38.977400856280966	0.9836736121763046	8.073044910348836E-4
220-224	1.3455074850581393E-4	6.727537425290697E-5	0.0	39.00270985207491	1.0828240587502387	8.073044910348836E-4
225-229	1.3455074850581393E-4	6.727537425290697E-5	0.0	39.03212264569828	1.2125578904595447	8.073044910348836E-4
230-234	1.3455074850581393E-4	6.727537425290697E-5	0.0	39.06461665146243	1.3139418794586755	8.073044910348836E-4
235-239	1.3455074850581393E-4	6.727537425290697E-5	0.0	39.103918925100984	1.3729692928281758	8.476697155866278E-4
240-241	1.3455074850581393E-4	6.727537425290697E-5	0.0	39.16560371575347	1.3753441135393036	8.745798652877906E-4
>>END_MODULE
