##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665024
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9149
Total Bases	2.1 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.07946223630998	32.0	25.0	33.0	18.0	33.0
2	29.75035523007979	31.0	29.0	33.0	18.0	33.0
3	28.93124931686523	31.0	28.0	33.0	18.0	33.0
4	29.731555361241664	31.0	29.0	33.0	15.0	33.0
5	29.510219696141654	31.0	29.0	33.0	15.0	33.0
6	31.505956935184173	35.0	29.0	37.0	16.0	38.0
7	33.76653186140562	37.0	33.0	38.0	26.0	38.0
8	33.5150289649142	37.0	33.0	38.0	16.0	38.0
9	34.55678216198492	38.0	34.0	38.0	26.0	38.0
10-14	35.054628921193576	38.0	35.4	38.0	27.8	38.0
15-19	35.186840091813316	38.0	36.0	38.0	28.0	38.0
20-24	35.23709695048639	38.0	36.0	38.0	28.2	38.0
25-29	35.07598644660619	38.0	36.0	38.0	27.2	38.0
30-34	35.03921740080883	38.0	36.0	38.0	27.0	38.0
35-39	34.989332167449994	38.0	35.8	38.0	26.6	38.0
40-44	34.916908951798014	38.0	35.4	38.0	26.6	38.0
45-49	34.95938353918461	38.0	35.4	38.0	27.0	38.0
50-54	34.87487157066346	38.0	35.2	38.0	26.6	38.0
55-59	34.75509891791452	38.0	35.0	38.0	25.4	38.0
60-64	34.56309979232704	38.0	34.8	38.0	25.0	38.0
65-69	34.33883484533829	38.0	34.2	38.0	24.2	38.0
70-74	34.06687069625096	38.0	34.0	38.0	19.2	38.0
75-79	33.81011188549143	38.0	34.0	38.0	17.2	38.0
80-84	33.62878490516029	38.0	33.6	38.0	15.2	38.0
85-89	33.42193143733876	38.0	33.0	38.0	15.0	38.0
90-94	33.223095615755554	38.0	33.0	38.0	15.0	38.0
95-99	33.07944203472888	38.0	32.6	38.0	15.0	38.0
100-104	32.95905309712774	38.0	31.2	38.0	15.0	38.0
105-109	32.719545194908655	37.4	31.0	38.0	15.0	38.0
110-114	32.50328302289856	37.0	31.0	38.0	15.0	38.0
115-119	32.41824889204274	37.0	31.0	38.0	15.0	38.0
120-124	32.659418192400565	36.6	31.0	38.0	16.6	38.0
125-129	31.230515724375852	34.0	29.4	35.6	18.2	36.2
130-134	31.843182083284727	36.0	29.0	37.0	15.8	38.0
135-139	32.669800536568296	38.0	29.0	38.0	16.0	38.0
140-144	32.66464481511723	38.0	29.0	38.0	16.0	38.0
145-149	32.63583343053774	38.0	29.0	38.0	16.0	38.0
150-154	32.60069987169019	38.0	29.0	38.0	16.0	38.0
155-159	32.609005015747115	38.0	29.0	38.0	16.0	38.0
160-164	32.574631036086814	38.0	29.0	38.0	16.0	38.0
165-169	32.54668208029442	38.0	29.0	38.0	16.0	38.0
170-174	32.533061343501785	38.0	29.0	38.0	16.0	38.0
175-179	32.44840922071553	38.0	28.6	38.0	16.0	38.0
180-184	32.39872689331414	38.0	28.2	38.0	16.0	38.0
185-189	32.252448811485934	38.0	28.2	38.0	15.0	38.0
190-194	32.043179575555655	38.0	27.8	38.0	15.0	38.0
195-199	31.886805845415303	38.0	27.2	38.0	15.0	38.0
200-204	31.74909874676643	37.4	26.8	38.0	15.0	38.0
205-209	31.5729499301461	37.0	26.0	38.0	15.0	38.0
210-214	31.51394300472707	37.0	26.0	38.0	15.0	38.0
215-219	31.47670774487592	37.0	25.8	38.0	15.0	38.0
220-224	31.47176089101753	37.0	25.8	38.0	14.4	38.0
225-229	31.446766267766474	37.0	25.6	38.0	14.0	38.0
230-234	31.410886566724052	37.0	25.2	38.0	14.0	38.0
235-239	31.604987610582736	37.0	26.6	38.0	14.0	38.0
240-244	32.07351809839398	37.0	29.0	38.0	14.4	38.0
245-249	33.27029041085474	37.8	31.8	38.0	19.0	38.0
250-252	35.46885583125211	37.666666666666664	34.0	38.0	30.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	8.0
17	15.0
18	16.0
19	21.0
20	34.0
21	69.0
22	128.0
23	209.0
24	260.0
25	315.0
26	410.0
27	419.0
28	376.0
29	344.0
30	318.0
31	320.0
32	395.0
33	466.0
34	618.0
35	876.0
36	1263.0
37	2268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.945112617537724	21.69254318827903	13.7983818062541	27.56396238792915
2	14.274784129413051	20.177068532080007	42.29970488577987	23.248442452727076
3	16.908951798010712	27.259809815280356	31.45698983495464	24.37424855175429
4	12.351076620395672	20.155208219477537	39.731118154989616	27.762597005137174
5	15.116406164608154	32.790468903705325	36.25532845119685	15.837796480489672
6	26.079352934746968	39.55623565416985	20.242649469887418	14.121761941195759
7	23.182861514919665	30.77932014427806	26.789813094327247	19.248005246475024
8	22.701934637665317	35.042081101759756	22.36309979232703	19.892884468247896
9	22.08984588479615	17.597551644988524	23.292163077932017	37.02043939228331
10-14	22.36965788610777	24.22778445731774	29.861187014974316	23.541370641600174
15-19	22.4221226363537	28.65449775931796	26.319816373374138	22.6035632309542
20-24	22.426494698874194	27.55492403541371	26.95813750136627	23.06044376434583
25-29	22.28100474389524	28.144196926305664	26.672933563605362	22.901864766193736
30-34	22.681561803156836	27.521752437584713	27.056097241047617	22.740588518210835
35-39	21.801766351871283	27.295383001049316	27.82660020986359	23.07625043721581
40-44	21.430757794394157	26.494381039835584	28.73540600813328	23.339455157636976
45-49	21.24398775688675	26.213379973764756	28.965894184521208	23.576738084827284
50-54	20.11411332633788	26.893144456103535	28.679171038824762	24.313571178733824
55-59	20.078697125368894	27.124275877145042	27.769155098917913	25.02787189856815
60-64	20.504973221117062	27.157066346048747	27.279484096622582	25.058476336211612
65-69	20.389113564323967	28.221663569789047	26.219258935402777	25.169963930484208
70-74	20.845994097715597	27.84566619302656	25.751448245709913	25.556891463547927
75-79	21.135757098481868	27.42048387802424	26.34203963774774	25.10171938574616
80-84	21.28722634935244	26.85337365503035	26.272653562115135	25.58674643350207
85-89	21.437196741827126	26.910663710013832	26.214679342217927	25.437460205941115
90-94	21.171062182434067	25.29969425686822	27.666454039548643	25.862789521149065
95-99	21.041611566604953	25.888212994798554	27.799083134973113	25.271092303623384
100-104	20.97084374101992	26.112425119918655	27.507239328897633	25.409491810163797
105-109	20.462507205888876	28.240432796771763	26.75269389384063	24.544366103498735
110-114	20.407981669743954	28.981380553022046	26.56552399172469	24.045113785509308
115-119	20.250083733392877	29.181645640281346	26.51780730155186	24.05046332477392
120-124	20.139947968063158	28.79025746837714	26.87494393110254	24.19485063245716
125-129	21.556637515287168	26.57313611925145	28.744490850774163	23.125735514687218
130-134	20.857636134571415	30.27390229107368	28.346787364098734	20.521674210256172
135-139	22.32513648453175	24.709532919602445	28.605758014091737	24.359572581774067
140-144	21.467898469578202	28.170492721164614	27.489268383725268	22.872340425531913
145-149	21.19027622247107	27.53826054497947	28.49477416946622	22.776689063083243
150-154	21.066629339305713	27.482269503546096	28.57642777155655	22.874673385591638
155-159	21.176772507757274	27.032638872687397	28.318129855586406	23.472458763968923
160-164	20.864350220521317	27.49632464471566	28.054045224371688	23.58527991039134
165-169	20.80657590547136	27.289073628657494	28.510379936015696	23.39397052985545
170-174	20.920394521572476	27.099985976721356	28.233534333660543	23.74608516804562
175-179	21.18192026951151	26.9488115291035	27.889294403892944	23.979973797492047
180-184	21.38758110228843	27.046588433701075	27.37919565268311	24.186634811327384
185-189	21.611412214635976	26.371976255836326	28.185636188733255	23.830975340794446
190-194	21.62047710911401	26.94913659248106	27.78196019385499	23.648426104549948
195-199	21.49068910104925	27.16702901975612	27.715284998582096	23.626996880612534
200-204	22.309155766944112	26.544589774078474	27.543400713436384	23.602853745541022
205-209	22.33758099872329	26.295377351672972	27.454050538385566	23.912991111218172
210-214	22.0849609375	26.81884765625	26.94580078125	24.150390625
215-219	22.386609878203693	27.480136491568906	26.790206480858803	23.3430471493686
220-224	22.737132212213236	27.968458927705615	26.873708117487944	22.42070074259321
225-229	22.513793922754033	28.0102431426384	27.19448771086882	22.28147522373875
230-234	22.437772504001323	28.34041613775595	27.242121529885754	21.979689828356975
235-239	21.890998449521692	28.505397419770063	27.949565573530705	21.654038557177543
240-244	22.07800450636286	28.697915013804703	27.320618196820156	21.90346228301228
245-249	21.01947933733843	29.65956672128163	27.722555980338615	21.598397961041325
250-252	20.203351534550933	30.69101864055733	28.902278290340806	20.203351534550933
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.3333333333333333
17	0.6666666666666666
18	0.3333333333333333
19	1.6666666666666665
20	1.9999999999999998
21	3.3333333333333335
22	8.499999999999996
23	13.166666666666671
24	15.333333333333343
25	18.333333333333336
26	25.999999999999975
27	43.66666666666669
28	49.000000000000064
29	63.00000000000016
30	66.16666666666683
31	83.66666666666657
32	89.99999999999982
33	98.66666666666636
34	102.83333333333297
35	109.66666666666622
36	114.33333333333283
37	122.16666666666606
38	127.166666666666
39	130.49999999999932
40	151.99999999999966
41	170.00000000000009
42	190.66666666666725
43	220.00000000000128
44	222.1666666666679
45	212.6666666666677
46	211.8333333333344
47	213.83333333333442
48	203.66666666666737
49	184.16666666666683
50	161.83333333333297
51	147.49999999999937
52	137.4999999999995
53	135.16666666666623
54	141.49999999999952
55	118.99999999999973
56	103.66666666666654
57	96.33333333333333
58	94.33333333333337
59	89.00000000000011
60	75.00000000000011
61	59.16666666666673
62	56.16666666666668
63	50.66666666666663
64	39.333333333333314
65	44.66666666666663
66	39.833333333333314
67	28.833333333333336
68	21.833333333333343
69	16.833333333333336
70	12.999999999999996
71	9.666666666666664
72	8.5
73	6.666666666666666
74	2.833333333333333
75	1.3333333333333333
76	1.0
77	0.3333333333333333
78	0.3333333333333333
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03279046890370532
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.004372062520494043
30-34	0.006558093780741065
35-39	0.0021860312602470216
40-44	0.01530221882172915
45-49	0.010930156301235108
50-54	0.0021860312602470216
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0021913485559013016
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.004614781144004245
130-134	0.006998716901901319
135-139	0.006998716901901319
140-144	0.002332905633967106
145-149	0.002332905633967106
150-154	0.002332905633967106
155-159	0.004665811267934212
160-164	0.00466690001166725
165-169	0.0023351391742947878
170-174	0.004674207721791156
175-179	0.004678800355588827
180-184	0.0117101503583306
185-189	0.007038288288288288
190-194	0.01176166168756322
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.007471607890017931
220-224	0.012757705654215147
225-229	0.015837402665962782
230-234	0.016554464187175807
235-239	0.07016107814190078
240-244	0.04123842152011166
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	15.0
80-84	17.0
85-89	17.0
90-94	17.0
95-99	26.0
100-104	21.0
105-109	33.0
110-114	33.0
115-119	34.0
120-124	74.0
125-129	289.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	5.0
165-169	7.0
170-174	9.0
175-179	7.0
180-184	15.0
185-189	16.0
190-194	35.0
195-199	35.0
200-204	97.0
205-209	106.0
210-214	143.0
215-219	177.0
220-224	223.0
225-229	312.0
230-234	373.0
235-239	462.0
240-244	661.0
245-249	1283.0
250-253	4607.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.56235654169855
#Duplication Level	Percentage of total
1	92.38168105803913
2	5.836703464859547
3	0.6230189091704011
4	0.0
5	0.05465078150617554
6	0.1311618756148213
7	0.0
8	0.0
9	0.0
>10	0.10930156301235108
>50	0.8634823477975735
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	79	0.8634823477975735	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	10	0.10930156301235108	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.010930156301235108	0.0	0.0
5	0.0	0.0	0.0	0.021860312602470216	0.0	0.0
6	0.0	0.0	0.0	0.021860312602470216	0.0	0.0
7	0.0	0.0	0.0	0.021860312602470216	0.0	0.0
8	0.0	0.0	0.0	0.021860312602470216	0.0	0.0
9	0.0	0.0	0.0	0.021860312602470216	0.0	0.0
10-14	0.0	0.0	0.0	0.021860312602470216	0.0	0.0
15-19	0.0	0.0	0.0	0.021860312602470216	0.0	0.0
20-24	0.0	0.0	0.0	0.03279046890370532	0.0	0.0
25-29	0.0	0.0	0.0	0.15520821947753852	0.0	0.0
30-34	0.0	0.0	0.0	0.9859000983714067	0.03934856268444639	0.0
35-39	0.0	0.0	0.0	3.025467264181878	0.04372062520494043	0.0
40-44	0.0	0.0	0.0	5.941632965351404	0.04809268772543447	0.0
45-49	0.0	0.0	0.0	8.964914198273036	0.07213903158815171	0.0
50-54	0.0	0.0	0.0	11.58815171056946	0.18799868838124384	0.0
55-59	0.0	0.0	0.0	14.257295879331076	0.3694392829817466	0.0
60-64	0.0	0.0	0.0	16.919881954311943	0.915947098043502	0.0
65-69	0.0	0.0	0.0	19.29391190294021	1.1083178489452397	0.0
70-74	0.0	0.0	0.0	21.53459394469341	1.1607825991911684	0.0
75-79	0.0	0.0	0.0	23.700950923598207	1.21106131817685	0.0
80-84	0.0	0.0	0.0	25.65963493277954	1.2613400371625314	0.0
85-89	0.0	0.0	0.0	27.697016067329763	1.318176849928954	0.0
90-94	0.0	0.0	0.0	29.454585200568367	1.4121761941195756	0.0
95-99	0.0	0.0	0.0	31.164061646081542	1.5717564761176086	0.0
100-104	0.0	0.0	0.0	32.82107334134878	1.8100338834845338	0.0
105-109	0.0	0.0	0.0	34.45403869275331	2.0898458847961523	0.0
110-114	0.0	0.0	0.0	36.11542245054104	2.308449010820855	0.0
115-119	0.0	0.0	0.0	36.9876489233796	2.485517542900864	0.0
120-124	0.0	0.0	0.0	36.99857907968084	2.5030057929828398	0.0
125-129	0.0	0.0	0.0	37.005137173461584	2.5030057929828398	0.0
130-134	0.0	0.0	0.0	37.01169526724232	2.5030057929828398	0.0
135-139	0.0	0.0	0.0	37.02918351732429	2.5030057929828398	0.0
140-144	0.0	0.0	0.0	37.04667176740627	2.5030057929828398	0.0
145-149	0.0	0.0	0.0	37.08164826757022	2.513935949284075	0.0
150-154	0.0	0.0	0.0	37.118810798994424	2.513935949284075	0.0
155-159	0.0	0.0	0.0	37.153787299158374	2.513935949284075	0.0
160-164	0.0	0.0	0.0	37.18876379932233	2.513935949284075	0.0
165-169	0.0	0.0	0.0	37.26090283091048	2.513935949284075	0.0
170-174	0.0	0.0	0.0	37.3221117061974	2.513935949284075	0.0
175-179	0.0	0.0	0.0	37.3964367690458	2.513935949284075	0.0
180-184	0.0	0.0	0.0	37.52978467592087	2.513935949284075	0.0
185-189	0.0	0.0	0.0	37.658760520275436	2.513935949284075	0.0
190-194	0.0	0.0	0.0	37.73527161438409	2.5183080118045686	0.0
195-199	0.0	0.0	0.0	37.83582905235545	2.533610230626298	0.0
200-204	0.0	0.0	0.0	37.92764236528582	2.5423543556672863	0.0
205-209	0.0	0.0	0.0	38.03038583451743	2.6123073559951906	0.0
210-214	0.0	0.0	0.0	38.12438517870805	2.6735162312821075	0.0
215-219	0.0	0.0	0.0	38.16591977265274	2.7500273253907532	0.0
220-224	0.0	0.0	0.0	38.242430866761396	2.8046781068969286	0.0
225-229	0.0	0.0	0.0	38.30801180456881	2.9030495136080443	0.0
230-234	0.0	0.0	0.0	38.37359274237622	3.0298393267023718	0.0
235-239	0.0	0.0	0.0	38.41731336758116	3.115094545852006	0.0
240-241	0.0	0.0	0.0	38.485080336648814	3.115094545852006	0.0
>>END_MODULE
