##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665025
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2428616
Total Bases	603.7 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.82767922141664	32.0	25.0	33.0	18.0	33.0
2	30.443596270468447	31.0	29.0	33.0	27.0	33.0
3	29.38559821725625	31.0	29.0	33.0	18.0	33.0
4	30.676097415153322	33.0	31.0	33.0	27.0	33.0
5	30.14496651590865	31.0	29.0	33.0	25.0	33.0
6	31.849872108229544	35.0	29.0	37.0	16.0	38.0
7	34.84120297321602	37.0	34.0	38.0	29.0	38.0
8	34.318410979751434	37.0	34.0	38.0	27.0	38.0
9	35.735972669207484	38.0	36.0	38.0	31.0	38.0
10-14	36.328227764290446	38.0	37.2	38.0	33.2	38.0
15-19	36.516348158786734	38.0	37.8	38.0	33.8	38.0
20-24	36.66080928397079	38.0	38.0	38.0	34.0	38.0
25-29	36.6486051314823	38.0	38.0	38.0	34.0	38.0
30-34	36.628928657309345	38.0	38.0	38.0	34.0	38.0
35-39	36.600131103476215	38.0	38.0	38.0	34.0	38.0
40-44	36.55944817953929	38.0	38.0	38.0	34.0	38.0
45-49	36.540253131824876	38.0	38.0	38.0	34.0	38.0
50-54	36.48482189032766	38.0	38.0	38.0	34.0	38.0
55-59	36.397918402909305	38.0	37.6	38.0	33.6	38.0
60-64	36.34489594073332	38.0	37.0	38.0	33.0	38.0
65-69	36.265424505150264	38.0	37.0	38.0	33.0	38.0
70-74	36.18994744331751	38.0	37.0	38.0	33.0	38.0
75-79	36.0804895301478	38.0	37.0	38.0	33.0	38.0
80-84	35.96816401419104	38.0	37.0	38.0	31.6	38.0
85-89	35.829637123040854	38.0	37.0	38.0	31.0	38.0
90-94	35.71712450052074	38.0	36.0	38.0	31.0	38.0
95-99	35.592430830807544	38.0	36.0	38.0	30.2	38.0
100-104	35.4593805681004	38.0	36.0	38.0	29.0	38.0
105-109	35.28581108023021	38.0	35.2	38.0	28.8	38.0
110-114	35.123462506138175	38.0	35.0	38.0	28.0	38.0
115-119	34.96775283048122	38.0	35.0	38.0	28.0	38.0
120-124	35.03973126973231	38.0	34.2	38.0	28.8	38.0
125-129	33.712544341933935	34.2	33.2	35.6	31.2	35.6
130-134	35.38552766088372	36.2	36.0	37.2	33.0	37.6
135-139	36.659408207286255	38.0	38.0	38.0	34.0	38.0
140-144	36.604780608598986	38.0	38.0	38.0	34.0	38.0
145-149	36.58676596596398	38.0	38.0	38.0	34.0	38.0
150-154	36.57089584765263	38.0	38.0	38.0	34.0	38.0
155-159	36.56159161320085	38.0	38.0	38.0	34.0	38.0
160-164	36.54781680842571	38.0	38.0	38.0	34.0	38.0
165-169	36.500228087315804	38.0	38.0	38.0	34.0	38.0
170-174	36.46831964811106	38.0	38.0	38.0	34.0	38.0
175-179	36.41543252006507	38.0	38.0	38.0	34.0	38.0
180-184	36.34766137598611	38.0	38.0	38.0	33.2	38.0
185-189	36.26157388992037	38.0	38.0	38.0	33.0	38.0
190-194	36.1540976616576	38.0	37.0	38.0	33.0	38.0
195-199	36.03370238780586	38.0	37.0	38.0	32.4	38.0
200-204	35.86757894744272	38.0	37.0	38.0	31.0	38.0
205-209	35.722420510768515	38.0	37.0	38.0	31.0	38.0
210-214	35.590614297560045	38.0	37.0	38.0	29.2	38.0
215-219	35.40032373923573	38.0	36.0	38.0	28.6	38.0
220-224	35.23673665054654	38.0	36.0	38.0	27.8	38.0
225-229	35.022187389478276	38.0	35.2	38.0	26.6	38.0
230-234	34.829411680018225	38.0	35.0	38.0	25.2	38.0
235-239	34.679569115025785	38.0	35.0	38.0	24.2	38.0
240-244	34.60395842907364	38.0	34.4	38.0	24.2	38.0
245-249	34.85688120621645	38.0	34.4	38.0	26.8	38.0
250-252	35.89744049800196	38.0	34.666666666666664	38.0	31.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	9.0
17	10.0
18	36.0
19	56.0
20	183.0
21	555.0
22	1540.0
23	3260.0
24	5841.0
25	9518.0
26	14168.0
27	19970.0
28	27766.0
29	36107.0
30	47261.0
31	61657.0
32	81710.0
33	113113.0
34	169039.0
35	261564.0
36	502728.0
37	1072525.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.50772190037626	19.44081867177959	12.223647817911845	26.827811609932304
2	16.39365795168936	20.252193018575188	38.365554702760754	24.988594326974706
3	18.91958218178584	26.496160776343398	28.6999673888338	25.88428965303696
4	13.734588425574598	19.017521523602156	38.01927272891976	29.22861732190349
5	16.805017524441983	32.77758287875194	34.41321118929033	16.004188407515745
6	30.279007333412938	36.02916894849317	19.400933044004596	14.290890674089294
7	25.492832131551467	29.527475731033643	25.137362184882257	19.842329952532637
8	24.78807880211561	33.297784951505285	21.143820654982367	20.77031559139674
9	23.947280218264407	15.965773070566176	20.594956629583788	39.49199008158563
10-14	24.734694711155207	22.319243889809034	28.092335068655238	24.853726330380518
15-19	24.257332098391192	27.023195682083596	25.096732056177846	23.62274016334737
20-24	24.080910010326054	25.933752672614695	25.91331303259283	24.072024284466426
25-29	24.242274259753728	25.851892522138808	25.711027350572767	24.19480586753469
30-34	24.122756377262153	25.802433550884274	25.87382594552305	24.20098412633053
35-39	24.041160994890948	25.560305162962155	26.077263501422	24.321270340724897
40-44	23.79142999697078	25.323952584743576	26.356382352258066	24.52823506602757
45-49	23.439777927520076	25.298990919544185	26.694141871477512	24.567089281458223
50-54	23.12612534266746	25.39655049737428	26.575553851414252	24.90177030854401
55-59	22.90433710033882	25.20796434706323	26.513194730787536	25.374503821810418
60-64	22.91095438887813	25.23839731125632	26.1064727541283	25.744175545737242
65-69	22.807837879681266	25.20086337238987	25.85206553856188	26.13923320936698
70-74	22.59718384045578	25.087451683032626	25.759328982455802	26.55603549405579
75-79	22.25777006667081	24.967491414356942	25.9221114876077	26.852627031364545
80-84	22.011424610860413	24.843974391557364	26.047707575290147	27.096893422292073
85-89	21.88950329843297	24.49159264227207	26.354776338976176	27.264127720318776
90-94	21.849154831034152	24.185409649672966	26.471531269831004	27.493904249461877
95-99	21.677544544115424	23.99504514020581	26.748978616106672	27.578431699572093
100-104	21.543856412342976	24.16522151310975	26.8363018180725	27.454620256474776
105-109	21.289476224727128	24.905783251065973	26.619732371056166	27.185008153150736
110-114	21.033041344374492	25.620715099581084	26.354780466212986	26.99146308983144
115-119	20.91275708602265	25.854815273782222	26.321546674993996	26.910880965201127
120-124	20.98729200451266	25.96545352397016	26.235161663764455	26.81209280775272
125-129	23.6238025976129	24.294624706858713	27.000765461770083	25.08080723375831
130-134	23.235893133276385	29.765556609098244	26.18363921728469	20.814911040340686
135-139	25.010200164741036	22.19505394776771	27.06689910277424	25.727846784717013
140-144	23.984016004507307	26.092233350983417	25.29007259692948	24.633678047579796
145-149	23.980756895976324	25.97196243517156	25.987834315435215	24.0594463534169
150-154	24.08529451684132	25.807485213015045	25.88031079859106	24.226909471552574
155-159	23.93138626580618	25.74474853199084	25.964011154520684	24.3598540476823
160-164	23.85260153519666	25.684950371525	26.091618847886238	24.370829245392102
165-169	23.808481265573835	25.634576356476206	26.007277350629497	24.54966502732046
170-174	23.795079625528672	25.751123759720464	25.983667126597716	24.47012948815315
175-179	23.87956851995177	25.489549934908094	25.916710649919033	24.7141708952211
180-184	23.875761257276938	25.46721838371544	25.922134308849987	24.734886050157634
185-189	23.816731599016897	25.24694203542649	26.329370325524355	24.60695604003226
190-194	23.776466651175447	25.546206206683337	26.02601201033013	24.65131513181109
195-199	23.77098920407385	25.402746509574403	26.2810507264314	24.545213559920345
200-204	23.878932320677595	25.37263985370198	25.94550623936319	24.802921586257238
205-209	24.076569886971242	24.940030219411305	26.193255764592827	24.790144129024625
210-214	24.03245493349094	25.386328288215577	25.76592539457191	24.815291383721576
215-219	24.187532556056443	25.81726956648172	25.459394719132522	24.535803158329315
220-224	24.42147559402327	25.74722325291992	25.78647307776182	24.044828075294987
225-229	24.19376264928425	26.05502789651455	25.56346821258356	24.187741241617637
230-234	24.318342006003217	26.066335466404915	25.521209252517004	24.094113275074868
235-239	24.40478655778017	26.214791021232482	25.553373481443103	23.827048939544245
240-244	24.4643932542675	25.901042877498764	25.555039830426946	24.079524037806785
245-249	23.887839693266034	26.43340440173893	25.56819927435084	24.110556630644194
250-252	23.334383174795516	26.6297907098067	25.724781819949026	24.31104429544875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.3333333333333333
12	0.3333333333333333
13	0.0
14	0.0
15	1.0
16	1.3333333333333333
17	3.5
18	7.166666666666664
19	15.500000000000009
20	21.499999999999993
21	35.66666666666664
22	71.66666666666676
23	117.99999999999943
24	187.50000000000088
25	311.6666666666665
26	500.99999999998937
27	822.5000000000218
28	1283.9999999999907
29	1891.3333333331877
30	2772.5000000001287
31	3969.8333333340024
32	5685.16666666602
33	7876.499999997371
34	10689.833333334744
35	14304.66666667459
36	18601.00000000434
37	23811.99999998551
38	29771.333333297363
39	36094.16666664141
40	42796.50000002319
41	49366.000000070635
42	55270.66666677994
43	60403.500000150234
44	64164.50000017728
45	65523.500000186774
46	64770.83333351437
47	62470.83333349764
48	58546.66666680239
49	53746.333333434166
50	49056.66666673368
51	44325.6666666998
52	39902.666666668105
53	36176.666666641395
54	32865.999999953405
55	30269.999999962685
56	28178.999999970143
57	26564.166666642574
58	25290.666666647136
59	24017.833333318365
60	22826.66666665596
61	21245.999999994987
62	19558.00000000107
63	17664.33333334126
64	15423.000000009855
65	13037.666666672234
66	10545.500000001086
67	8386.499999997177
68	6379.999999998751
69	4715.000000000246
70	3288.1666666670167
71	2281.8333333332307
72	1564.9999999999313
73	978.6666666667055
74	595.9999999999959
75	360.3333333333311
76	217.00000000000148
77	116.16666666666622
78	61.333333333333464
79	28.49999999999997
80	18.166666666666675
81	7.333333333333331
82	3.333333333333333
83	2.6666666666666665
84	1.1666666666666665
85	0.3333333333333333
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0321582333312471
2	0.0
3	0.0
4	1.235271446782859E-4
5	3.2940571914209576E-4
6	2.470542893565718E-4
7	0.0
8	4.117571489276197E-5
9	8.235142978552394E-5
10-14	7.246925821126107E-4
15-19	8.235142978552393E-6
20-24	7.411628680697154E-5
25-29	0.0021658426033592795
30-34	0.00271759718292229
35-39	0.0026517160390938706
40-44	0.010985680733388894
45-49	0.006077535518171666
50-54	9.223360135978681E-4
55-59	1.0705685872118112E-4
60-64	9.058657276407634E-5
65-69	0.0
70-74	7.32927725091163E-4
75-79	5.352873352416464E-4
80-84	0.002215299616802578
85-89	0.0018529947607190808
90-94	0.004669656015184041
95-99	0.002256650509652987
100-104	0.001639026421600095
105-109	9.060804754319574E-4
110-114	3.871898485741693E-4
115-119	0.0012523794179022858
120-124	7.582032856739384E-4
125-129	0.0011566476880058526
130-134	0.002448158587743327
135-139	0.0018940145830852093
140-144	0.0010421215609988487
145-149	0.0012819749361493774
150-154	0.0015218289406375056
155-159	0.0014308521667526694
160-164	0.0018526694402672078
165-169	0.001662449888715108
170-174	0.002166994197087295
175-179	0.0032753732870976346
180-184	0.003565083620649295
185-189	0.0028293003669652215
190-194	0.0022508104572653846
195-199	0.0012335116130151205
200-204	0.0013503587895019296
205-209	0.001915943971494735
210-214	0.0039055949558991854
215-219	0.012370604642477856
220-224	0.013310495237539986
225-229	0.01491194983370136
230-234	0.027615681396626526
235-239	0.07176130704559937
240-244	0.030133491726868668
245-249	1.712106773065859E-4
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	33.0
80-84	52.0
85-89	66.0
90-94	53.0
95-99	86.0
100-104	164.0
105-109	296.0
110-114	310.0
115-119	470.0
120-124	1187.0
125-129	7755.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	2.0
155-159	7.0
160-164	8.0
165-169	22.0
170-174	31.0
175-179	90.0
180-184	257.0
185-189	501.0
190-194	866.0
195-199	1331.0
200-204	2351.0
205-209	3653.0
210-214	6190.0
215-219	9693.0
220-224	15507.0
225-229	24855.0
230-234	38089.0
235-239	52539.0
240-244	87476.0
245-249	273599.0
250-253	1901077.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.39240716305166
#Duplication Level	Percentage of total
1	93.13808595244112
2	6.004726280489547
3	0.5962712398156655
4	0.13490059568915075
5	0.04227966694462234
6	0.030975457879657303
7	0.018059613450980175
8	0.010363246447457421
9	0.003523510660982457
>10	0.020814436180785482
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	4.117571489276197E-5	0.0	0.0	4.117571489276197E-5	0.0	0.0
3	8.235142978552394E-5	0.0	0.0	8.235142978552394E-5	0.0	0.0
4	8.235142978552394E-5	4.117571489276197E-5	0.0	1.235271446782859E-4	0.0	0.0
5	8.235142978552394E-5	8.235142978552394E-5	0.0	1.235271446782859E-4	0.0	0.0
6	8.235142978552394E-5	8.235142978552394E-5	0.0	1.235271446782859E-4	0.0	0.0
7	8.235142978552394E-5	8.235142978552394E-5	0.0	1.235271446782859E-4	0.0	0.0
8	8.235142978552394E-5	1.235271446782859E-4	0.0	1.235271446782859E-4	0.0	0.0
9	8.235142978552394E-5	1.6470285957104788E-4	0.0	1.235271446782859E-4	0.0	0.0
10-14	9.058657276407634E-5	1.6470285957104788E-4	4.117571489276197E-5	2.8823000424933385E-4	0.0	0.0
15-19	1.235271446782859E-4	1.6470285957104788E-4	8.235142978552394E-5	6.835168672198487E-4	0.0	0.0
20-24	1.235271446782859E-4	2.0587857446380985E-4	9.058657276407634E-5	0.0033599383352493767	2.4705428935657183E-5	0.0
25-29	1.235271446782859E-4	2.470542893565718E-4	1.482325736139431E-4	0.05118141361170313	4.941085787131437E-5	0.0
30-34	1.235271446782859E-4	3.458760050992006E-4	1.6470285957104788E-4	0.43230383065910793	6.50576295305639E-4	0.0
35-39	1.235271446782859E-4	3.8705171999196255E-4	1.6470285957104788E-4	1.2871116718328461	7.658682970053726E-4	0.0
40-44	1.317622876568383E-4	4.117571489276197E-4	1.6470285957104788E-4	2.875613106394753	9.470414425335253E-4	0.0
45-49	1.9764343148525747E-4	4.117571489276197E-4	1.729380025496003E-4	4.682469357032977	0.0012682120186970687	4.117571489276197E-5
50-54	2.3881914637801944E-4	4.2822743488472447E-4	2.0587857446380985E-4	6.582778010191814	0.0018940828850670505	4.117571489276197E-5
55-59	2.470542893565718E-4	4.529328638203817E-4	2.0587857446380985E-4	8.487747754276509	0.0027258323259008424	4.117571489276197E-5
60-64	2.470542893565718E-4	4.529328638203817E-4	2.0587857446380985E-4	10.45032232349618	0.003837576628005415	4.117571489276197E-5
65-69	2.635245753136766E-4	5.270491506273532E-4	2.0587857446380985E-4	12.41983911824677	0.004430506922461188	5.764600084986676E-5
70-74	3.047002902064386E-4	5.764600084986676E-4	2.1411371744236226E-4	14.433125697928366	0.004998731787981303	8.235142978552394E-5
75-79	3.2940571914209576E-4	6.094005804128772E-4	2.470542893565718E-4	16.4582626483561	0.005682248655201151	8.235142978552394E-5
80-84	3.2940571914209576E-4	6.176357233914295E-4	2.882300042493338E-4	18.543087915092382	0.006406941237313763	8.235142978552394E-5
85-89	3.7058143403485773E-4	6.176357233914295E-4	2.882300042493338E-4	20.628431995836316	0.007246925821126108	1.399974306353907E-4
90-94	3.7058143403485773E-4	6.258708663699819E-4	2.882300042493338E-4	22.713306673430463	0.010672745300203903	1.6470285957104788E-4
95-99	3.7058143403485773E-4	6.917520101984011E-4	2.882300042493338E-4	24.802793031092605	0.04155453146977538	1.6470285957104788E-4
100-104	3.7058143403485773E-4	8.317494408337917E-4	2.882300042493338E-4	26.902556847191978	0.10857212502923475	1.6470285957104788E-4
105-109	3.7058143403485773E-4	8.646900127480013E-4	2.882300042493338E-4	29.008068793090388	0.22165710841071626	1.9764343148525747E-4
110-114	4.0352200594906726E-4	8.97630584662211E-4	3.2940571914209576E-4	31.096608109309994	0.3274539902561788	2.1411371744236226E-4
115-119	4.941085787131436E-4	9.058657276407635E-4	3.2940571914209576E-4	32.306029442283176	0.4044443419626651	2.470542893565718E-4
120-124	5.352842936059056E-4	9.470414425335253E-4	3.2940571914209576E-4	32.33281012724943	0.41407945924757145	2.470542893565718E-4
125-129	5.682248655201151E-4	9.882171574262873E-4	3.2940571914209576E-4	32.33310659239665	0.41414534039139994	2.635245753136766E-4
130-134	5.764600084986676E-4	0.0010540983012547064	3.623462910563054E-4	32.33376540383494	0.41423592696416395	3.211705761635434E-4
135-139	6.094005804128772E-4	0.0010788037301903634	3.7058143403485773E-4	32.334934794137894	0.4143100432509709	3.5411114807775297E-4
140-144	6.176357233914295E-4	0.0011117443021045731	3.9528686297051494E-4	32.33659829301956	0.4143512189658637	3.7058143403485773E-4
145-149	6.176357233914295E-4	0.001128214588061678	4.776382927560389E-4	32.339414711918224	0.4144088649667136	3.7058143403485773E-4
150-154	6.176357233914295E-4	0.0011529200169973352	5.517545795630103E-4	32.344553441136846	0.4144335703956492	3.7058143403485773E-4
155-159	6.588114382841915E-4	0.0012188011608257544	6.011654374343247E-4	32.35072979837076	0.41449945153947765	3.7058143403485773E-4
160-164	6.999871531769534E-4	0.001235271446782859	6.176357233914295E-4	32.358668476202084	0.41451592182543473	3.7058143403485773E-4
165-169	6.999871531769534E-4	0.0012517417327399638	6.176357233914295E-4	32.36888005349549	0.41458180296926317	3.7058143403485773E-4
170-174	7.411628680697155E-4	0.0013093877335898306	6.341060093485344E-4	32.3780540027736	0.41463121382713447	3.788165770134101E-4
175-179	8.15279154876687E-4	0.001317622876568383	6.588114382841915E-4	32.38931967836825	0.41466415439904863	4.199922919061721E-4
180-184	8.235142978552394E-4	0.001317622876568383	6.588114382841915E-4	32.404809982310915	0.4148041518296841	4.941085787131436E-4
185-189	8.235142978552394E-4	0.001334093162525488	6.588114382841915E-4	32.42157673341525	0.4151088521198905	4.941085787131436E-4
190-194	8.482197267908967E-4	0.0013752688774182494	6.752817242412963E-4	32.437972903085544	0.41566060669945354	5.105788646702484E-4
195-199	8.729251557265538E-4	0.0013999743063539069	6.999871531769534E-4	32.455357289913266	0.41715940272155	5.435194365844579E-4
200-204	9.058657276407635E-4	0.0014082094493324594	7.246925821126107E-4	32.47386989132905	0.4252463131264885	5.764600084986676E-4
205-209	9.058657276407635E-4	0.0015317365940107452	7.411628680697155E-4	32.49274483903589	0.44524124027841366	5.764600084986676E-4
210-214	9.058657276407635E-4	0.0015646771659249548	7.411628680697155E-4	32.51175154903039	0.47205486581658035	5.764600084986676E-4
215-219	9.058657276407635E-4	0.001605852880817717	7.411628680697155E-4	32.53627580482053	0.5096729989426076	6.50576295305639E-4
220-224	9.305711565764206E-4	0.0016882043106032404	7.658682970053726E-4	32.56418470437483	0.566536661209512	6.752817242412963E-4
225-229	9.470414425335253E-4	0.0016882043106032404	7.823385829624774E-4	32.590537161906205	0.6305401924388211	6.999871531769534E-4
230-234	9.470414425335253E-4	0.0017046745965603454	7.823385829624774E-4	32.61621433771334	0.6872885627040256	6.999871531769534E-4
235-239	9.470414425335253E-4	0.0017623205974102122	7.823385829624774E-4	32.64945960991775	0.7272043007210691	7.411628680697155E-4
240-241	9.470414425335253E-4	0.0017705557403887647	7.823385829624774E-4	32.72040536667798	0.7283778085955128	7.411628680697155E-4
>>END_MODULE
