##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665030
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4836505
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.947033239911878	32.0	25.0	33.0	18.0	33.0
2	30.44586286998566	31.0	29.0	33.0	27.0	33.0
3	29.3934601535613	31.0	29.0	33.0	18.0	33.0
4	30.62517541075632	33.0	31.0	33.0	27.0	33.0
5	30.08747701077534	31.0	29.0	33.0	25.0	33.0
6	31.932619112354892	35.0	29.0	37.0	16.0	38.0
7	34.87765276785613	37.0	34.0	38.0	29.0	38.0
8	34.405212855150566	37.0	34.0	38.0	27.0	38.0
9	35.735272681409405	38.0	36.0	38.0	31.0	38.0
10-14	36.33125134782245	38.0	37.2	38.0	33.2	38.0
15-19	36.52504967946896	38.0	37.8	38.0	34.0	38.0
20-24	36.65461861406119	38.0	38.0	38.0	34.0	38.0
25-29	36.63786511127354	38.0	38.0	38.0	34.0	38.0
30-34	36.617688227345994	38.0	38.0	38.0	34.0	38.0
35-39	36.58774838442223	38.0	38.0	38.0	34.0	38.0
40-44	36.54639666453359	38.0	38.0	38.0	34.0	38.0
45-49	36.5248760830393	38.0	38.0	38.0	34.0	38.0
50-54	36.47349993435343	38.0	38.0	38.0	34.0	38.0
55-59	36.3858059487171	38.0	37.4	38.0	33.6	38.0
60-64	36.32879941197207	38.0	37.0	38.0	33.0	38.0
65-69	36.246050567506906	38.0	37.0	38.0	33.0	38.0
70-74	36.15018977546803	38.0	37.0	38.0	33.0	38.0
75-79	36.00003421011736	38.0	37.0	38.0	32.0	38.0
80-84	35.833227338741025	38.0	37.0	38.0	31.0	38.0
85-89	35.66460990737736	38.0	36.0	38.0	30.4	38.0
90-94	35.54210031487126	38.0	36.0	38.0	29.0	38.0
95-99	35.40108825675735	38.0	36.0	38.0	29.0	38.0
100-104	35.25524221414025	38.0	35.2	38.0	28.0	38.0
105-109	35.06045028625717	38.0	35.0	38.0	27.6	38.0
110-114	34.87955953287578	38.0	35.0	38.0	27.0	38.0
115-119	34.72022345401238	38.0	34.2	38.0	26.4	38.0
120-124	34.83078058963747	38.0	34.0	38.0	28.0	38.0
125-129	33.65988307578088	34.2	33.2	35.6	31.0	35.8
130-134	35.207950419678816	36.2	36.0	37.2	32.6	38.0
135-139	36.43709856346191	38.0	38.0	38.0	34.0	38.0
140-144	36.388251279739244	38.0	38.0	38.0	33.2	38.0
145-149	36.372048378893034	38.0	38.0	38.0	33.0	38.0
150-154	36.361467915741954	38.0	38.0	38.0	33.0	38.0
155-159	36.35782252384536	38.0	38.0	38.0	33.0	38.0
160-164	36.347149963637044	38.0	38.0	38.0	33.0	38.0
165-169	36.29857728252305	38.0	38.0	38.0	33.0	38.0
170-174	36.25927737012623	38.0	37.6	38.0	33.0	38.0
175-179	36.19456808366721	38.0	37.0	38.0	33.0	38.0
180-184	36.10693952111389	38.0	37.0	38.0	33.0	38.0
185-189	35.990112803080606	38.0	37.0	38.0	31.6	38.0
190-194	35.84045421218015	38.0	37.0	38.0	31.0	38.0
195-199	35.66253947431522	38.0	37.0	38.0	29.4	38.0
200-204	35.42567667838351	38.0	36.2	38.0	28.8	38.0
205-209	35.206896314116285	38.0	36.0	38.0	27.4	38.0
210-214	35.01232095486746	38.0	35.8	38.0	26.4	38.0
215-219	34.758760211074204	38.0	35.0	38.0	24.6	38.0
220-224	34.53462825036776	38.0	35.0	38.0	23.2	38.0
225-229	34.25430551608333	38.0	34.0	38.0	23.0	38.0
230-234	34.0099937307161	38.0	34.0	38.0	21.6	38.0
235-239	33.84540487249186	38.0	33.6	38.0	21.0	38.0
240-244	33.783519334061026	38.0	33.0	38.0	21.0	38.0
245-249	34.158587562814034	38.0	33.2	38.0	23.8	38.0
250-252	35.569601059201084	37.666666666666664	34.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	5.0
17	16.0
18	32.0
19	62.0
20	367.0
21	1249.0
22	3625.0
23	7824.0
24	14054.0
25	22590.0
26	33515.0
27	46651.0
28	63624.0
29	83656.0
30	107722.0
31	140077.0
32	184047.0
33	255315.0
34	389075.0
35	582720.0
36	1047500.0
37	1852778.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.99242707225492	19.244283281356584	12.565880996613341	27.197408649775152
2	16.194678957658603	19.75572811390726	38.89214423003761	25.15744869839652
3	18.199763175703406	25.93302549345115	29.062687312738948	26.804524018106495
4	13.08646478104729	19.32297749964282	37.40511994104829	30.185437778261598
5	16.281328278698272	33.68890837043845	33.69777844860472	16.33198490225856
6	29.859772475822894	36.37738222519315	19.57480796535178	14.188037333632172
7	25.183577810836542	29.789383035890587	25.72820662854685	19.298832524726016
8	24.333294393368764	32.868031770875874	21.611018700487232	21.187655135268134
9	23.815145739627525	16.063861857185213	19.664501327612392	40.45649107557487
10-14	24.91038801788729	22.176712376520808	27.609224146590112	25.30367545900179
15-19	24.390613651412067	26.78660837884416	24.87196539120565	23.950812578538123
20-24	24.248909521618643	25.757289174248527	25.68250370743214	24.31129759670069
25-29	24.391138436375538	25.703063481092503	25.49919365970645	24.406604422825513
30-34	24.270892653618716	25.526532556351057	25.722170349243793	24.480404440786433
35-39	24.11562604882372	25.210434668113923	26.102822893796557	24.5711163892658
40-44	23.722859258779522	24.85955398301178	26.55271017936321	24.86487657884549
45-49	23.111846689362327	24.8690311249761	27.0465847187354	24.97253746692617
50-54	22.640603501863012	25.002778888781922	26.8502064813611	25.50641112799396
55-59	22.35381550804472	24.806906910023844	26.61906393741559	26.220213644515848
60-64	22.49580895275509	24.84300217099142	25.89195492608291	26.76923395017058
65-69	22.62296017475429	25.104351179208955	25.120970618246023	27.15171802779073
70-74	22.939814342898323	25.384112188063984	24.478008171506104	27.19806529753159
75-79	23.3177888547867	25.630029004781196	24.121817599872962	26.930364540559136
80-84	23.462700202828284	25.48050394972793	24.155302935847054	26.901492911596737
85-89	23.478590228153983	24.96798364097214	24.535907533512965	27.01751859736091
90-94	23.4544569145114	24.413988761431135	24.856703594377613	27.27485072967985
95-99	23.25866043539884	24.200563184659583	25.283317930101795	27.25745844983979
100-104	23.030643421905648	24.64026833285599	25.396501291930885	26.93258695330748
105-109	22.673711032105015	25.96601910623838	24.977036526875516	26.383233334781085
110-114	22.334380660882907	27.130850135792926	24.595126248988368	25.939642954335795
115-119	22.228052308397565	27.507605469259016	24.519030649595237	25.745311572748186
120-124	22.350905894445418	27.594513722544388	24.427026021706233	25.62755436130396
125-129	23.865198478946006	24.727772604171815	26.504058593544034	24.902970323338145
130-134	22.963259793720916	29.753508662199156	26.193510123903565	21.089721420176367
135-139	25.144448282060694	22.194973668723623	26.577174361124197	26.083403688091483
140-144	24.115947902123732	25.73207841046577	25.244938648260018	24.907035039150482
145-149	24.095911215958584	25.694270735270074	25.90617364057065	24.303644408200693
150-154	24.10376045231205	25.551647233119873	25.885199972212945	24.459392342355134
155-159	23.946330112676698	25.38235978353649	25.992886596446674	24.678423507340142
160-164	23.81641636841012	25.37022169400849	26.15762978296214	24.655732154619255
165-169	23.755860897526798	25.234833961728725	26.046723881806045	24.96258125893844
170-174	23.756980656976744	25.469259749472318	25.97944964096925	24.794309952581692
175-179	23.915586788603605	25.109569045190867	25.87148228049293	25.103361885712598
180-184	23.95856314240688	25.12212710765671	25.81431307665013	25.10499667328628
185-189	23.906298723556663	24.77009655275209	26.397850638471198	24.925754085220046
190-194	23.844009359541616	25.315598685416873	25.924300918824322	24.916091036217193
195-199	23.86960538405746	25.036620016699533	26.33417549968382	24.759599099559185
200-204	24.133375031859604	24.9358868483643	25.837314550431717	25.09342356934438
205-209	24.408783085356305	24.38752910467241	26.10962359697207	25.094064212999218
210-214	24.391155234997328	25.24059636789272	25.414885066776876	24.953363330333076
215-219	24.55158016333881	25.75625578759932	25.056620394511324	24.635543654550546
220-224	25.021189139610527	25.700069639657237	25.41663623470263	23.862104986029607
225-229	24.524001069788646	26.256705812640245	25.169260918481978	24.05003219908913
230-234	24.815918647030443	26.391052255721654	25.0990487550538	23.693980342194106
235-239	24.69223224565064	26.543820172256016	25.190141550499597	23.57380603159375
240-244	24.933002601285036	26.13333363532002	25.076351908298722	23.857311855096217
245-249	24.035673898766973	26.79360816460889	25.232707484798013	23.938010451826123
250-252	23.499282467661846	27.20251925923703	25.033244599074813	24.264953674026312
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.3333333333333333
11	0.3333333333333333
12	0.0
13	0.0
14	0.0
15	2.333333333333333
16	2.6666666666666665
17	6.999999999999997
18	11.66666666666667
19	25.666666666666643
20	47.00000000000004
21	92.66666666666649
22	165.83333333333363
23	255.1666666666694
24	385.66666666666265
25	621.0
26	988.0000000000416
27	1549.3333333332635
28	2339.4999999999336
29	3460.166666667101
30	4939.166666666712
31	6929.16666666493
32	9650.999999999452
33	13032.666666672227
34	17428.00000000878
35	22895.666666655736
36	29661.83333329803
37	37691.49999998555
38	47483.33333338951
39	58588.16666680287
40	70702.6666667916
41	83713.99999993698
42	96912.33333307972
43	109298.4999995677
44	119804.16666608394
45	126232.99999932709
46	129452.4999992816
47	129145.33333261992
48	125070.4999993471
49	118127.33333278279
50	109980.66666623543
51	101746.33333302001
52	93563.16666647032
53	86348.6666665717
54	80525.99999998619
55	75547.83333339021
56	71006.66666678799
57	66493.16666685231
58	61672.16666682379
59	56087.000000117456
60	50584.3333334115
61	44376.00000003352
62	37886.99999998679
63	31446.83333329177
64	25369.66666664697
65	19920.49999999987
66	15224.00000000947
67	11288.83333333569
68	8138.499999997201
69	5763.333333332663
70	4016.000000000671
71	2682.3333333334085
72	1761.9999999998881
73	1210.0000000000102
74	727.3333333333447
75	437.6666666666597
76	252.16666666666933
77	142.49999999999955
78	82.16666666666664
79	49.50000000000006
80	24.333333333333318
81	8.33333333333333
82	3.5
83	1.0
84	0.3333333333333333
85	0.3333333333333333
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03413622026649409
2	1.860847864315244E-4
3	1.6540869905024393E-4
4	8.270434952512197E-5
5	4.135217476256098E-4
6	1.6540869905024393E-4
7	0.0
8	0.0
9	6.202826214384147E-5
10-14	8.766661049662927E-4
15-19	4.135217476256098E-6
20-24	7.856913204886586E-5
25-29	0.0017657378623613538
30-34	0.00244804874594361
35-39	0.0027830013615203542
40-44	0.010726754133408319
45-49	0.005781034031806025
50-54	8.146378428224514E-4
55-59	7.443391457260977E-5
60-64	1.0338043690640244E-4
65-69	0.0
70-74	8.353139302037318E-4
75-79	9.014806530259687E-4
80-84	0.0026259012113633786
85-89	0.0020263107770207094
90-94	0.00532226984925446
95-99	0.002932090468844988
100-104	0.0018693616464973434
105-109	0.001282281435129382
110-114	4.716302802526416E-4
115-119	0.0011172674386101889
120-124	8.77550313333065E-4
125-129	0.0011707943116999883
130-134	0.00246937137661842
135-139	0.00175017735615548
140-144	8.813244643823318E-4
145-149	0.0012138997339605703
150-154	0.0013926591150453661
155-159	0.0015547908718976204
160-164	0.0016711953844410381
165-169	0.001712774742891517
170-174	0.002190875018021714
175-179	0.0032469083383641012
180-184	0.003858356730857582
185-189	0.00285289144290599
190-194	0.0020886568251542517
195-199	0.0011783555652525844
200-204	0.0011005355022331283
205-209	0.0015035012786025457
210-214	0.003611072116838542
215-219	0.010435384189533865
220-224	0.011361862761265525
225-229	0.012813444806000445
230-234	0.02494018790470714
235-239	0.06555674605774447
240-244	0.026992209761452928
245-249	1.432988835993404E-4
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	54.0
80-84	53.0
85-89	69.0
90-94	121.0
95-99	184.0
100-104	522.0
105-109	851.0
110-114	958.0
115-119	1254.0
120-124	3123.0
125-129	18375.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	3.0
155-159	4.0
160-164	18.0
165-169	26.0
170-174	92.0
175-179	224.0
180-184	805.0
185-189	1744.0
190-194	3020.0
195-199	4628.0
200-204	7462.0
205-209	11397.0
210-214	20400.0
215-219	31999.0
220-224	51567.0
225-229	79705.0
230-234	116789.0
235-239	155046.0
240-244	240730.0
245-249	637052.0
250-253	3448230.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.38436270042646
#Duplication Level	Percentage of total
1	72.52791981232158
2	15.352943618262612
3	5.705179266014944
4	2.7828405781648247
5	1.4727268412252355
6	0.7884909819845493
7	0.46020219437145177
8	0.2960608073876343
9	0.18571858764224827
>10	0.42306596189309725
>50	0.004851350731809613
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.067608738128049E-5	0.0	0.0
5	0.0	0.0	0.0	2.067608738128049E-5	0.0	0.0
6	0.0	0.0	0.0	2.067608738128049E-5	0.0	0.0
7	0.0	0.0	0.0	2.067608738128049E-5	0.0	0.0
8	0.0	0.0	2.067608738128049E-5	4.135217476256098E-5	0.0	0.0
9	0.0	2.067608738128049E-5	2.067608738128049E-5	8.270434952512197E-5	2.067608738128049E-5	0.0
10-14	2.481130485753659E-5	4.5487392238817076E-5	6.616347962009757E-5	1.3232695924019514E-4	2.8946522333792684E-5	1.6540869905024392E-5
15-19	4.135217476256098E-5	6.202826214384147E-5	1.28191741763939E-4	5.375782719132928E-4	6.616347962009757E-5	2.067608738128049E-5
20-24	4.135217476256098E-5	6.202826214384147E-5	1.6540869905024393E-4	0.004958125754031062	8.683956700137807E-5	2.067608738128049E-5
25-29	6.616347962009757E-5	6.616347962009757E-5	2.191665262415732E-4	0.0841516756418116	1.2819174176393906E-4	2.8946522333792684E-5
30-34	9.924521943014636E-5	9.511000195389027E-5	2.5224826605162203E-4	0.7508562484686773	2.3157217867034147E-4	4.135217476256098E-5
35-39	1.2405652428768295E-4	1.1992130681142683E-4	3.721695728630488E-4	2.2404939103753643	2.7705957090915856E-4	4.5487392238817076E-5
40-44	1.2405652428768295E-4	1.4059739419270732E-4	3.721695728630488E-4	4.842620859484276	3.2254696314797565E-4	6.202826214384147E-5
45-49	1.4059739419270732E-4	1.530030466214756E-4	4.2592740005437807E-4	7.740829379893126	4.4246826995940244E-4	6.202826214384147E-5
50-54	1.530030466214756E-4	1.6540869905024393E-4	4.838204447219634E-4	10.659383170285157	8.022321903936831E-4	7.029869709635367E-5
55-59	1.7781435147901222E-4	1.695439165265E-4	5.375782719132928E-4	13.547960769191803	0.0012529708953055978	8.270434952512197E-5
60-64	2.067608738128049E-4	1.860847864315244E-4	5.499839243420611E-4	16.406764802269407	0.0017161152526462807	9.511000195389027E-5
65-69	2.274369611940854E-4	1.860847864315244E-4	5.747952291995977E-4	19.210847502483716	0.0020055804759842076	1.2405652428768295E-4
70-74	2.729243534329025E-4	1.860847864315244E-4	6.78175666106E-4	21.99780213191137	0.0022909104818458785	1.4473261166896343E-4
75-79	3.2254696314797565E-4	1.860847864315244E-4	7.236630583448172E-4	24.722509332668942	0.002485265703229915	1.4473261166896343E-4
80-84	3.5562870295802445E-4	1.860847864315244E-4	7.319334932973293E-4	27.423505196417658	0.002613457444993854	1.4473261166896343E-4
85-89	4.052513126730976E-4	1.860847864315244E-4	7.939617554411707E-4	30.06476370850438	0.0031799822392409396	1.4473261166896343E-4
90-94	4.135217476256098E-4	1.860847864315244E-4	8.063674078699391E-4	32.652682050364874	0.008349004084561063	1.4473261166896343E-4
95-99	4.21792182578122E-4	1.9022000390778052E-4	8.270434952512196E-4	35.18281899842965	0.06577063395985325	1.4473261166896343E-4
100-104	4.4660348743565863E-4	2.067608738128049E-4	8.601252350612683E-4	37.65511252443655	0.20093848760623634	1.654086990502439E-4
105-109	4.7555000976945124E-4	2.1089609128906102E-4	8.808013224425489E-4	40.05701637856262	0.4221602169335088	1.860847864315244E-4
110-114	5.086317495795001E-4	2.3984261362285366E-4	8.890717573950612E-4	42.3757568740237	0.6241035623864754	1.860847864315244E-4
115-119	5.210374020082684E-4	2.481130485753659E-4	8.890717573950612E-4	43.68963125231961	0.7677816935989935	1.860847864315244E-4
120-124	5.375782719132928E-4	2.481130485753659E-4	9.304239321576221E-4	43.71720488245127	0.7852157704788892	1.860847864315244E-4
125-129	5.582543592945733E-4	2.6465391848039027E-4	9.304239321576221E-4	43.717775542463	0.7852860691759856	1.9435522138403662E-4
130-134	5.789304466758537E-4	2.93600440814183E-4	9.345591496338783E-4	43.718623262045625	0.7853522326556057	2.191665262415732E-4
135-139	5.789304466758537E-4	3.1841174567171957E-4	9.511000195389027E-4	43.72047170425752	0.7854183961352257	2.481130485753659E-4
140-144	6.368234913434391E-4	3.3081739810048786E-4	9.67640889443927E-4	43.72356898214723	0.7854886948323221	2.687891359566464E-4
145-149	6.78175666106E-4	3.3081739810048786E-4	9.924521943014636E-4	43.72895717051879	0.7855465878769896	2.8946522333792685E-4
150-154	6.947165360110245E-4	3.3081739810048786E-4	0.0010379395865402804	43.73666108067706	0.7855962104867047	3.060060932429513E-4
155-159	7.195278408685611E-4	3.5149348548176837E-4	0.0010999678486841222	43.74716039784928	0.7856541035313723	3.1014131071920736E-4
160-164	7.277982758210732E-4	3.5562870295802445E-4	0.001174401763256732	43.76043444594806	0.7857244022284687	3.1427652819546344E-4
165-169	7.484743632023538E-4	3.8457522529181717E-4	0.0011785369807329879	43.774659594066385	0.7857698896207076	3.597639204342805E-4
170-174	7.732856680598905E-4	3.928456602443293E-4	0.001182672198209244	43.78917834262552	0.7858319178828513	3.9698087772058546E-4
175-179	7.898265379649147E-4	3.928456602443293E-4	0.0012033482855905247	43.80548764035187	0.785922892667329	4.135217476256098E-4
180-184	8.187730602987074E-4	4.2592740005437807E-4	0.0012571061127818538	43.82752834950031	0.7861337887586181	4.2592740005437807E-4
185-189	8.642604525375245E-4	4.548739223881708E-4	0.001302593505020671	43.852773852192854	0.7864025778945748	4.548739223881708E-4
190-194	9.097478447763416E-4	4.796852272457073E-4	0.0013232695924019512	43.87702690269109	0.7870972944305856	4.796852272457074E-4
195-199	9.67640889443927E-4	5.04496532103244E-4	0.0013604865496882564	43.902375785820546	0.7895742896988631	5.293078369607805E-4
200-204	9.841817593489513E-4	5.375782719132928E-4	0.0013852978545457928	43.928642687229726	0.801570555597482	5.541191418183171E-4
205-209	9.924521943014636E-4	5.582543592945733E-4	0.00143078524678461	43.95315625642897	0.834267720182239	5.789304466758537E-4
210-214	0.0010007226292539759	5.747952291995977E-4	0.0014845430739759392	43.976582263431965	0.878349138479129	6.864461010585123E-4
215-219	0.001013128281682744	5.789304466758537E-4	0.0015300304662147564	44.00757985363398	0.935460627043702	7.526095806786098E-4
220-224	0.0010172634991590002	5.789304466758537E-4	0.0015424361186435246	44.041269470413035	1.0138354038711836	7.815561030124027E-4
225-229	0.0010338043690640245	5.996065340571343E-4	0.0015507065535960369	44.07242833409663	1.1040120913759008	8.105026253461952E-4
230-234	0.0010710213263503293	6.202826214384147E-4	0.0015507065535960369	44.10165605121881	1.1836481095336406	8.43584365156244E-4
235-239	0.0011165087185891465	6.202826214384147E-4	0.0015631122060248055	44.138595948934196	1.2414708555041294	8.683956700137805E-4
240-241	0.0011165087185891465	6.202826214384147E-4	0.0015713826409773173	44.21240131045042	1.2432324581490146	8.683956700137806E-4
>>END_MODULE
