##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665031
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4877923
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.2161786891675	32.0	25.0	33.0	18.0	33.0
2	30.719805950196427	33.0	30.0	33.0	27.0	33.0
3	29.584597378843412	31.0	29.0	33.0	18.0	33.0
4	30.866340038577896	33.0	31.0	33.0	27.0	33.0
5	30.295512864799218	31.0	30.0	33.0	27.0	33.0
6	32.23642480621363	35.0	29.0	37.0	16.0	38.0
7	35.04496893452398	37.0	35.0	38.0	29.0	38.0
8	34.59399605118818	37.0	34.0	38.0	28.0	38.0
9	35.88074945012457	38.0	36.0	38.0	31.0	38.0
10-14	36.4299990385252	38.0	37.4	38.0	33.6	38.0
15-19	36.60866881252533	38.0	38.0	38.0	34.0	38.0
20-24	36.734893888238915	38.0	38.0	38.0	34.0	38.0
25-29	36.71667355142752	38.0	38.0	38.0	34.0	38.0
30-34	36.69698775482925	38.0	38.0	38.0	34.0	38.0
35-39	36.668050028669995	38.0	38.0	38.0	34.0	38.0
40-44	36.6298281871198	38.0	38.0	38.0	34.0	38.0
45-49	36.613426657206354	38.0	38.0	38.0	34.0	38.0
50-54	36.55869549396331	38.0	38.0	38.0	34.0	38.0
55-59	36.48207915541102	38.0	38.0	38.0	34.0	38.0
60-64	36.4284828194295	38.0	38.0	38.0	34.0	38.0
65-69	36.3583904870987	38.0	37.2	38.0	33.4	38.0
70-74	36.29102923518883	38.0	37.0	38.0	33.0	38.0
75-79	36.188249595156336	38.0	37.0	38.0	33.0	38.0
80-84	36.06123841608458	38.0	37.0	38.0	32.8	38.0
85-89	35.92593228517832	38.0	37.0	38.0	31.0	38.0
90-94	35.816559862890884	38.0	37.0	38.0	31.0	38.0
95-99	35.69270429656963	38.0	36.2	38.0	31.0	38.0
100-104	35.553019687147696	38.0	36.0	38.0	29.0	38.0
105-109	35.375195919082756	38.0	35.8	38.0	28.8	38.0
110-114	35.20410446969422	38.0	35.0	38.0	28.0	38.0
115-119	35.032659980954314	38.0	35.0	38.0	28.0	38.0
120-124	35.11319625915391	38.0	34.6	38.0	29.0	38.0
125-129	33.8706326987221	34.2	33.2	35.6	31.8	35.6
130-134	35.612934523054214	36.2	36.0	37.2	33.8	37.8
135-139	36.88500401753938	38.0	38.0	38.0	35.0	38.0
140-144	36.85128543779251	38.0	38.0	38.0	35.0	38.0
145-149	36.84119792803144	38.0	38.0	38.0	35.0	38.0
150-154	36.83682570994867	38.0	38.0	38.0	35.0	38.0
155-159	36.83520147504723	38.0	38.0	38.0	35.0	38.0
160-164	36.829357396990346	38.0	38.0	38.0	35.0	38.0
165-169	36.79874621106787	38.0	38.0	38.0	35.0	38.0
170-174	36.77871675624641	38.0	38.0	38.0	35.0	38.0
175-179	36.744204624385205	38.0	38.0	38.0	35.0	38.0
180-184	36.69223639295333	38.0	38.0	38.0	34.2	38.0
185-189	36.62504934025991	38.0	38.0	38.0	34.0	38.0
190-194	36.53739891523551	38.0	38.0	38.0	34.0	38.0
195-199	36.428230033421656	38.0	38.0	38.0	34.0	38.0
200-204	36.27195946931427	38.0	38.0	38.0	33.4	38.0
205-209	36.12655519984615	38.0	37.8	38.0	33.0	38.0
210-214	35.98873151770943	38.0	37.0	38.0	32.2	38.0
215-219	35.78782625312032	38.0	37.0	38.0	31.0	38.0
220-224	35.61720884238777	38.0	37.0	38.0	29.4	38.0
225-229	35.394049313242725	38.0	36.4	38.0	28.6	38.0
230-234	35.191918705135855	38.0	36.0	38.0	27.6	38.0
235-239	35.020622233664845	38.0	35.8	38.0	26.4	38.0
240-244	34.93169310981738	38.0	35.0	38.0	26.0	38.0
245-249	35.15329869411961	38.0	35.0	38.0	28.6	38.0
250-252	36.04544163259029	38.0	35.333333333333336	38.0	32.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	16.0
18	22.0
19	59.0
20	164.0
21	607.0
22	1794.0
23	4261.0
24	8137.0
25	13766.0
26	21490.0
27	32117.0
28	45275.0
29	61306.0
30	82008.0
31	108672.0
32	147995.0
33	207300.0
34	316655.0
35	502019.0
36	983850.0
37	2340407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.68826579453143	20.739379054809497	14.728684078200146	25.843671072458918
2	13.90274613287565	21.199779249900676	41.68581077895182	23.21166383827185
3	16.4097772221663	27.779792529954943	31.302879984025942	24.507550263852814
4	12.171098757910153	19.7964783263645	40.18156964009981	27.850853275625536
5	14.340784754769773	34.793351901410155	36.60099231104495	14.264871032775128
6	27.81478703272242	38.76548332492467	20.59540266126326	12.824326981089643
7	22.911144763867735	32.23414965754892	27.59758610375769	17.257119474825657
8	22.56312779025007	36.20998117436458	23.244831867989717	17.98205916739563
9	23.12320697214921	17.052199686669855	21.9848328858067	37.83976045537423
10-14	23.093335307960928	23.905700216024094	29.716628229900937	23.28433624611404
15-19	22.53720876997925	28.735207893622583	26.720477278585765	22.007106057812397
20-24	22.303821678719075	27.68449301087234	27.60197421884315	22.409711091565438
25-29	22.505988635175047	27.609515770417726	27.34995323528166	22.534542359125563
30-34	22.349717397001857	27.5726614934021	27.561193200995994	22.516427908600047
35-39	22.324176086860433	27.273329632745497	27.74835275595252	22.654141524441552
40-44	22.04007073104893	26.929514614471046	28.107796821646197	22.922617832833826
45-49	21.62972676197482	26.87519322821718	28.510307828438762	22.98477218136923
50-54	21.297321938013468	26.969595186898193	28.337687952337383	23.395394922750956
55-59	21.022965987659735	26.698941093267297	28.295597765095593	23.98249515397738
60-64	21.160433611225116	26.668734488771644	27.725980633540786	24.444851266462454
65-69	21.153039931134625	26.646287774530265	27.245846234145148	24.954826060189962
70-74	21.25868686165451	26.52236720039814	26.92736251434946	25.291583423597892
75-79	21.15354400443472	26.363602816966086	26.859191877113624	25.62366130148557
80-84	21.047768894305598	26.10072838130158	26.98589926981459	25.865603454578235
85-89	20.93500890292933	25.65030172701166	27.276561403560567	26.13812796649845
90-94	21.02101460557589	25.216296551011226	27.37106727812282	26.391621565290073
95-99	20.830848984780474	24.858181472850983	27.725108426668367	26.585861115700176
100-104	20.76224324399574	24.947416293238117	27.812086836812284	26.47825362595386
105-109	20.45868678172951	25.880782050928225	27.515289470540633	26.14524169680163
110-114	20.238879884713747	26.809110215656045	27.130332266981444	25.821677632648765
115-119	20.072941472575557	27.09749085206482	27.08823298743293	25.74133468792669
120-124	20.180724844092442	27.320409368476838	26.977011076429886	25.52185471100083
125-129	21.571526344274467	25.771317486632388	29.06907427269732	23.588081896395824
130-134	21.114819959302437	31.94001644733933	28.13954003966266	18.80562355369557
135-139	23.506410199721163	23.877088531031117	28.63725181903569	23.979249450212027
140-144	22.36600020747071	27.73491759127004	26.96580344758399	22.933278753675264
145-149	22.256686930878825	27.75105004453388	27.70072885516703	22.291534169420267
150-154	22.400647687111153	27.540835314166547	27.58082011327943	22.47769688544287
155-159	22.251730626358864	27.423199791968628	27.659443894503877	22.66562568716863
160-164	22.12291634283346	27.352995217394003	27.846696979442893	22.677391460329645
165-169	22.072269638896135	27.278703111201896	27.714574360312966	22.934452889589004
170-174	22.082850052193955	27.365771380043626	27.69015212136803	22.861226446394397
175-179	22.182116905328243	27.03687764991189	27.594417835691704	23.186587609068166
180-184	22.26153539532782	27.01764135907429	27.52796576336913	23.192857482228753
185-189	22.173199136286776	26.71039236122453	27.979611918176143	23.136796584312545
190-194	22.122878954644428	27.089866764502506	27.602579087127722	23.184675193725337
195-199	22.16846298702178	26.827158689218873	27.891564377964812	23.11281394579454
200-204	22.39243611301973	26.677754284881576	27.455752017382785	23.474057584715908
205-209	22.70469266570771	26.10734633079247	27.655171958866255	23.53278904463357
210-214	22.72761321795802	26.63704430239555	27.102154953909725	23.533187525736704
215-219	22.942638939787845	27.135638222084967	26.72967303763268	23.192049800494505
220-224	23.207503794961703	26.930482327605365	27.118149613462407	22.74386426397053
225-229	23.033694150027898	27.440697294264893	26.75044419405091	22.7751643616563
230-234	23.185173931643067	27.436066239029234	26.76272032582321	22.616039503504492
235-239	23.341929337417575	27.608563885715032	26.643458974751272	22.40604780211612
240-244	23.423502985836016	27.105037408873383	26.763695573532853	22.707764031757744
245-249	22.901395587475893	27.846828216748282	26.605598946540336	22.64617724923549
250-252	22.26921438336843	28.0961794592709	26.98885445619787	22.64575170116281
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.3333333333333333
12	0.6666666666666666
13	1.6666666666666665
14	4.666666666666666
15	8.166666666666664
16	11.166666666666668
17	22.999999999999986
18	40.00000000000001
19	66.33333333333348
20	101.33333333333299
21	172.33333333333383
22	265.66666666666913
23	427.1666666666602
24	682.1666666666727
25	1068.8333333333737
26	1643.8333333332444
27	2469.666666666656
28	3691.500000000539
29	5472.666666666224
30	7790.333333330807
31	10904.333333335086
32	15161.500000009448
33	20533.833333330742
34	27255.666666639754
35	35800.99999997257
36	46052.50000004681
37	57901.50000013267
38	72191.1666667663
39	88473.66666653073
40	106616.33333293481
41	124873.49999933681
42	141662.16666619224
43	156194.83333328037
44	166024.1666668988
45	171172.6666670474
46	169719.33333366946
47	162049.66666678115
48	148781.99999972942
49	131317.16666591493
50	112474.99999952196
51	93358.4999997983
52	75506.33333338764
53	59840.33333347759
54	47140.33333338637
55	36558.666666643854
56	28178.166666637055
57	22126.49999999202
58	17545.50000000841
59	13845.00000000697
60	11135.666666668785
61	9141.333333331857
62	7654.833333330938
63	6504.166666665304
64	5532.666666666173
65	4683.8333333336
66	3901.833333333966
67	3257.1666666670117
68	2671.1666666667484
69	2087.9999999998195
70	1620.8333333332503
71	1236.3333333333364
72	893.0000000000297
73	621.6666666666658
74	426.9999999999937
75	278.3333333333353
76	163.33333333333346
77	92.33333333333316
78	50.666666666666735
79	30.166666666666632
80	14.000000000000005
81	5.333333333333333
82	3.1666666666666665
83	1.6666666666666665
84	0.3333333333333333
85	0.0
86	0.0
87	0.3333333333333333
88	0.3333333333333333
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033108353698900125
2	1.8450475745517098E-4
3	2.870074004858215E-4
4	3.0750792909195164E-4
5	3.4850898630421184E-4
6	2.870074004858215E-4
7	0.0
8	0.0
9	2.0500528606130107E-5
10-14	8.446217785725604E-4
15-19	8.200211442452042E-6
20-24	7.790200870329442E-5
25-29	0.0020582530720554626
30-34	0.00258716671009362
35-39	0.0028577736876945373
40-44	0.010611073606532944
45-49	0.0055556432522612595
50-54	7.66719769869266E-4
55-59	1.0660274875187656E-4
60-64	1.0250264303065055E-4
65-69	0.0
70-74	8.077208270815263E-4
75-79	7.62621821660805E-4
80-84	0.002410890830312032
85-89	0.0019721938705116546
90-94	0.005162221265678248
95-99	0.0027308123834714998
100-104	0.001652471675282345
105-109	0.001107199779806669
110-114	2.9528521783744177E-4
115-119	9.147031142359593E-4
120-124	7.590078954462932E-4
125-129	0.0011302582335921542
130-134	0.002525486747467161
135-139	0.002056585299240359
140-144	0.0011475745969761204
145-149	0.001312101420915349
150-154	0.0016699472629831717
155-159	0.0017151928450545378
160-164	0.001842704672560223
165-169	0.0019455404962403357
170-174	0.002463819590820853
175-179	0.0031631143192704454
180-184	0.0038296105487630357
185-189	0.002879729101415091
190-194	0.0021232428930616372
195-199	0.0014201369324844673
200-204	0.0013509582866092602
205-209	0.0018717046248914288
210-214	0.004286169801860858
215-219	0.012283271286109856
220-224	0.014022620442450708
225-229	0.015115287620352962
230-234	0.029300228255281714
235-239	0.07589362038201046
240-244	0.03141410053021515
245-249	1.6596609217899017E-4
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	42.0
80-84	42.0
85-89	62.0
90-94	79.0
95-99	82.0
100-104	247.0
105-109	505.0
110-114	620.0
115-119	949.0
120-124	1922.0
125-129	10944.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	5.0
160-164	13.0
165-169	20.0
170-174	59.0
175-179	103.0
180-184	351.0
185-189	839.0
190-194	1500.0
195-199	2268.0
200-204	3941.0
205-209	5935.0
210-214	11092.0
215-219	18830.0
220-224	30352.0
225-229	51281.0
230-234	82781.0
235-239	112352.0
240-244	181027.0
245-249	535704.0
250-253	3823975.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.17473861374415
#Duplication Level	Percentage of total
1	68.7227255050824
2	15.101344737210647
3	6.228338213783082
4	3.4129021826708414
5	2.08627002190312
6	1.3467118410872816
7	0.8563986552706444
8	0.5604345284798269
9	0.4186895806226997
>10	1.2161262022495696
>50	0.028733247546943706
>100	0.021325284092922638
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.100105721226022E-5	0.0	0.0
3	0.0	0.0	0.0	4.100105721226022E-5	0.0	0.0
4	0.0	0.0	0.0	4.100105721226022E-5	0.0	0.0
5	2.050052860613011E-5	0.0	0.0	4.100105721226022E-5	0.0	0.0
6	2.050052860613011E-5	0.0	2.050052860613011E-5	4.100105721226022E-5	0.0	0.0
7	2.050052860613011E-5	0.0	2.050052860613011E-5	6.150158581839033E-5	0.0	0.0
8	2.050052860613011E-5	0.0	4.100105721226022E-5	8.200211442452044E-5	0.0	0.0
9	2.050052860613011E-5	0.0	4.100105721226022E-5	1.8450475745517098E-4	0.0	0.0
10-14	8.200211442452043E-5	0.0	4.100105721226022E-5	2.7060697760091743E-4	4.100105721226022E-5	2.050052860613011E-5
15-19	1.1890306591555463E-4	0.0	4.100105721226022E-5	4.797123693834445E-4	7.790200870329442E-5	2.050052860613011E-5
20-24	1.7630454601271894E-4	8.200211442452044E-6	4.100105721226022E-5	0.0013981360509380733	9.020232586697249E-5	3.69009514910342E-5
25-29	3.034078233707256E-4	2.050052860613011E-5	4.100105721226022E-5	0.03515430645379191	1.0250264303065054E-4	4.100105721226022E-5
30-34	3.649094091891159E-4	2.050052860613011E-5	4.100105721226022E-5	0.40762431059284865	3.0750792909195164E-4	5.74014800971643E-5
35-39	3.7720972635279403E-4	2.050052860613011E-5	7.790200870329442E-5	1.3544699250070162	3.8540993779524605E-4	6.150158581839033E-5
40-44	3.977102549589241E-4	2.050052860613011E-5	8.200211442452044E-5	3.290601348155762	3.977102549589241E-4	6.150158581839033E-5
45-49	4.510116293348624E-4	2.870074004858215E-5	9.430243158819848E-5	5.459471172464182	5.002128979895747E-4	6.150158581839033E-5
50-54	4.633119464985405E-4	4.100105721226022E-5	1.2300317163678066E-4	7.802476586858792	8.487218842937865E-4	6.150158581839033E-5
55-59	5.535142723655129E-4	4.100105721226022E-5	1.3940359452168472E-4	10.140586475022259	0.0011808304477130944	7.38019029820684E-5
60-64	6.068156467414511E-4	4.100105721226022E-5	1.4350370024291076E-4	12.58188372387182	0.0015457398569022102	8.200211442452044E-5
65-69	6.642171268386155E-4	4.100105721226022E-5	1.5580401740658884E-4	14.990277624308543	0.0018655481031578398	8.200211442452044E-5
70-74	7.093182897721017E-4	7.79020087032944E-5	1.92704968897623E-4	17.49018588444303	0.002136155080758757	9.84025373094245E-5
75-79	7.462192412631359E-4	1.0250264303065054E-4	2.1320549750375312E-4	19.967035150001344	0.0023903616354747706	1.0250264303065054E-4
80-84	7.58519558426814E-4	1.0250264303065054E-4	2.255058146674312E-4	22.49674707862342	0.002718370093172852	1.0250264303065054E-4
85-89	7.790200870329442E-4	1.0250264303065054E-4	2.7060697760091743E-4	25.014720404565633	0.0030094775993799	1.0250264303065054E-4
90-94	8.528219900150125E-4	1.0250264303065054E-4	3.2800845769808177E-4	27.541160448822172	0.004858625279652837	1.1070285447310258E-4
95-99	8.897229415060466E-4	1.0250264303065054E-4	3.8540993779524605E-4	30.059556085653668	0.0361916332012621	1.312033830792327E-4
100-104	9.020232586697248E-4	1.0250264303065054E-4	4.0591046640137607E-4	32.54628250589442	0.11457745437966119	1.4350370024291076E-4
105-109	9.143235758334029E-4	1.0250264303065054E-4	4.1001057212260216E-4	35.010569047522885	0.27098828743299147	1.4350370024291076E-4
110-114	9.47124421603211E-4	1.148029601943286E-4	4.1001057212260216E-4	37.40064777570289	0.41643543778776343	1.4350370024291076E-4
115-119	9.635248444881152E-4	1.2300317163678066E-4	4.264109950075063E-4	38.80669703068294	0.5268102838031679	1.4350370024291076E-4
120-124	0.0010127261131428276	1.2300317163678066E-4	4.305111007287323E-4	38.84047780172012	0.5410130500214947	1.4350370024291076E-4
125-129	0.0010824279104036695	1.3940359452168472E-4	4.305111007287323E-4	38.840887812292245	0.5410458508672645	1.5990412312781485E-4
130-134	0.0011070285447310258	1.6400422884904088E-4	4.4281141789241033E-4	38.841564329736244	0.5410909520301981	1.8450475745517098E-4
135-139	0.001119328861894704	1.6400422884904088E-4	4.838124751046706E-4	38.84262625711804	0.5411401532988529	1.8450475745517098E-4
140-144	0.00114392949622206	1.6400422884904088E-4	5.166133208744786E-4	38.84512732160799	0.5412180553075561	1.92704968897623E-4
145-149	0.0012095311877616765	1.6400422884904088E-4	5.453140609230608E-4	38.84865341252824	0.5413697592192415	2.0500528606130108E-4
150-154	0.0012792329850225188	1.7630454601271896E-4	6.027155410202251E-4	38.85441816117228	0.5415337634480906	2.3780613183110925E-4
155-159	0.0013448346765621351	1.8450475745517098E-4	6.150158581839033E-4	38.862659373671946	0.5415870648224665	2.6240676615846536E-4
160-164	0.0014022361566592993	1.8450475745517098E-4	6.355163867900334E-4	38.87306544199242	0.5416280658796786	3.321085634193078E-4
165-169	0.0014596376367564637	1.8450475745517098E-4	6.560169153961635E-4	38.88498444932402	0.5416649668311697	4.305111007287322E-4
170-174	0.0015908410198356968	1.8450475745517098E-4	6.560169153961635E-4	38.89655904777505	0.541759269262758	5.002128979895747E-4
175-179	0.001668743028538991	1.8450475745517098E-4	6.560169153961635E-4	38.909876191157586	0.541812570637134	5.207134265957047E-4
180-184	0.0017548452486847374	2.0500528606130108E-4	6.560169153961635E-4	38.92803965950262	0.5418945727515584	5.535142723655129E-4
185-189	0.0018163468345031276	2.3370602610988325E-4	6.806175497235196E-4	38.94854838832019	0.5420708772975711	5.74014800971643E-4
190-194	0.0018655481031578398	2.460063432735613E-4	7.421191355419099E-4	38.969553229930035	0.5423988857552692	6.027155410202251E-4
195-199	0.001902449054648874	2.5420655471601334E-4	7.790200870329442E-4	38.992087410973895	0.5434403126084606	6.437165982324854E-4
200-204	0.0019516503233035867	2.665068718796914E-4	8.241212499664304E-4	39.016347736526384	0.5500660834539619	6.765174440022936E-4
205-209	0.002009051803400751	2.665068718796914E-4	8.692224128999165E-4	39.03976754040603	0.5713005309841914	6.765174440022936E-4
210-214	0.002009051803400751	2.7470708332214347E-4	8.815227300635947E-4	39.062851135616526	0.601186201586208	7.175185012145538E-4
215-219	0.0020418526491705587	2.870074004858215E-4	9.22523787275855E-4	39.093105815733466	0.6428596761367492	7.626196641480401E-4
220-224	0.002091053917825271	2.870074004858215E-4	9.430243158819851E-4	39.12610756668361	0.708522869262184	7.831201927541703E-4
225-229	0.002152555503643661	3.157081405344037E-4	9.430243158819851E-4	39.15799408887758	0.7890407454156205	7.995206156390744E-4
230-234	0.0021894564551346958	3.2800845769808177E-4	9.51224527324437E-4	39.18888018527558	0.8571353012337424	7.995206156390744E-4
235-239	0.0021976566665771477	3.2800845769808177E-4	9.840253730942453E-4	39.22827810115084	0.9075092001247251	7.995206156390744E-4
240-241	0.002224307353765117	3.2800845769808177E-4	9.840253730942453E-4	39.310962883177936	0.9089729378672029	7.995206156390743E-4
>>END_MODULE
