##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665032
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1654878
Total Bases	410.3 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.49634232855836	32.0	25.0	33.0	18.0	33.0
2	30.759551459382504	33.0	30.0	33.0	27.0	33.0
3	29.75478192350131	31.0	29.0	33.0	18.0	33.0
4	30.89220957677847	33.0	31.0	33.0	27.0	33.0
5	30.42986310773362	33.0	31.0	33.0	27.0	33.0
6	32.59861029030539	36.0	29.0	37.0	16.0	38.0
7	35.21632652074654	37.0	35.0	38.0	29.0	38.0
8	34.86059999589094	38.0	34.0	38.0	29.0	38.0
9	35.964471701237194	38.0	36.0	38.0	31.0	38.0
10-14	36.469128358706804	38.0	37.6	38.0	33.6	38.0
15-19	36.637871190504676	38.0	38.0	38.0	34.0	38.0
20-24	36.75059950038614	38.0	38.0	38.0	34.0	38.0
25-29	36.73328885875575	38.0	38.0	38.0	34.0	38.0
30-34	36.714793718932754	38.0	38.0	38.0	34.0	38.0
35-39	36.688805217061315	38.0	38.0	38.0	34.0	38.0
40-44	36.656151813003746	38.0	38.0	38.0	34.0	38.0
45-49	36.63887247277442	38.0	38.0	38.0	34.0	38.0
50-54	36.5909616297999	38.0	38.0	38.0	34.0	38.0
55-59	36.517510414665004	38.0	38.0	38.0	34.0	38.0
60-64	36.46728701451104	38.0	38.0	38.0	34.0	38.0
65-69	36.39549380679422	38.0	37.8	38.0	33.8	38.0
70-74	36.31189429069696	38.0	37.4	38.0	33.0	38.0
75-79	36.188266843159425	38.0	37.0	38.0	33.0	38.0
80-84	36.045498639062885	38.0	37.0	38.0	32.6	38.0
85-89	35.900683612912445	38.0	37.0	38.0	31.0	38.0
90-94	35.78924172382808	38.0	37.0	38.0	31.0	38.0
95-99	35.66288844727996	38.0	36.0	38.0	31.0	38.0
100-104	35.531410632184496	38.0	36.0	38.0	29.0	38.0
105-109	35.360154283027086	38.0	35.8	38.0	28.8	38.0
110-114	35.19940342207569	38.0	35.0	38.0	28.0	38.0
115-119	35.04148025740056	38.0	35.0	38.0	28.0	38.0
120-124	35.12731801045223	38.0	34.6	38.0	29.0	38.0
125-129	33.77426686338943	34.2	33.4	35.6	31.2	35.6
130-134	35.35548553280424	36.2	36.0	37.2	33.0	38.0
135-139	36.60627884617137	38.0	38.0	38.0	34.0	38.0
140-144	36.56233263222852	38.0	38.0	38.0	34.0	38.0
145-149	36.551293602170496	38.0	38.0	38.0	34.0	38.0
150-154	36.541276968515504	38.0	38.0	38.0	34.0	38.0
155-159	36.5382317121254	38.0	38.0	38.0	34.0	38.0
160-164	36.53209824215328	38.0	38.0	38.0	34.0	38.0
165-169	36.4904567063548	38.0	38.0	38.0	34.0	38.0
170-174	36.46434971062734	38.0	38.0	38.0	34.0	38.0
175-179	36.4136275495304	38.0	38.0	38.0	34.0	38.0
180-184	36.33617268393517	38.0	38.0	38.0	33.2	38.0
185-189	36.24773700998106	38.0	38.0	38.0	33.0	38.0
190-194	36.12587910969101	38.0	37.0	38.0	33.0	38.0
195-199	35.98299276207463	38.0	37.0	38.0	31.6	38.0
200-204	35.783443218647434	38.0	37.0	38.0	31.0	38.0
205-209	35.60038663372385	38.0	37.0	38.0	29.2	38.0
210-214	35.430643506189035	38.0	36.4	38.0	28.8	38.0
215-219	35.20458681156616	38.0	36.0	38.0	27.4	38.0
220-224	35.00538313788364	38.0	35.8	38.0	25.8	38.0
225-229	34.75367339791822	38.0	35.0	38.0	24.4	38.0
230-234	34.529750383822666	38.0	35.0	38.0	23.2	38.0
235-239	34.34672811386539	38.0	34.0	38.0	23.0	38.0
240-244	34.276130783557946	38.0	34.0	38.0	23.0	38.0
245-249	34.58661290599027	38.0	34.0	38.0	25.2	38.0
250-252	35.796647016495676	38.0	34.666666666666664	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	5.0
18	9.0
19	25.0
20	92.0
21	334.0
22	946.0
23	2106.0
24	3815.0
25	6265.0
26	9670.0
27	13582.0
28	18848.0
29	25093.0
30	32529.0
31	42302.0
32	56025.0
33	77404.0
34	116530.0
35	179985.0
36	332625.0
37	736687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.23541679134048	21.67976270569667	14.266552299226811	25.818268203736043
2	15.366951964981062	20.584684290545034	40.48986147602599	23.55850226844791
3	17.14178843827385	26.687167723947564	31.139107871656694	25.031935966121893
4	12.77402388338931	19.776853101807145	40.128009745757076	27.321113269046464
5	14.735692708745626	35.21037566024704	35.59258021612535	14.461351414881987
6	27.506082034040052	39.24335024902198	20.048233279391663	13.202334437546304
7	23.899828265286022	31.796785019802066	26.253476087058985	18.04991062785293
8	22.95522691098679	34.668900063932206	22.68427038126073	19.691602643820268
9	23.512652012991918	17.078389606465745	21.996887982476018	37.41207039806632
10-14	23.596095774902537	23.661430464722287	28.938650895457485	23.80382286491769
15-19	23.04214570500061	28.295668925443447	26.160635406356235	22.501549963199704
20-24	22.88764561361146	27.141341448985727	27.09402673142559	22.87698620597722
25-29	23.022466558434772	27.110929878614375	26.861770109988765	23.00483345296209
30-34	22.959748683526566	26.96312593294642	27.05274196661449	23.02438341691252
35-39	22.800118441169186	26.677749376068842	27.35225974873552	23.169872434026455
40-44	22.507946787496273	26.29271953517926	27.7210925670961	23.47824111022837
45-49	22.008453107852663	26.22487877513331	28.158240148515567	23.608427968498454
50-54	21.604157257513354	26.338841190331365	27.969311244538964	24.087690307616317
55-59	21.353056059789374	26.07639484771064	27.783024226588577	24.787524865911404
60-64	21.531616685284373	26.036509558841924	27.14074925967272	25.291124496200982
65-69	21.67025001238762	26.111725456498906	26.604390172568614	25.61363435854486
70-74	22.05949516812199	26.280495107814914	26.143057815392424	25.51695190867067
75-79	22.376019891009395	26.42721896051275	25.90418925319402	25.29257189528384
80-84	22.391485978664186	26.392105262839845	25.91148321339839	25.304925545097586
85-89	22.38485023439004	25.92862558253059	26.19546051869047	25.491063664388896
90-94	22.496619372530404	25.386638870664953	26.344708937281563	25.772032819523083
95-99	22.28757714119817	25.152596315980887	26.6928277042221	25.866998838598835
100-104	22.16536894457295	25.44829822720997	26.724432916657754	25.661899911559328
105-109	21.899223869107516	26.539085305735423	26.32446584487343	25.237224980283635
110-114	21.589838539787614	27.63061998671022	25.943015948705693	24.83652552479647
115-119	21.48636683218616	27.98212621645461	25.889232945207812	24.642274006151414
120-124	21.568757669364736	28.135875014032273	25.82317444925735	24.472192867345637
125-129	22.41656816592818	25.770231695816943	28.339957540853796	23.473242597401082
130-134	21.468297000988702	31.491009550154985	27.591088595219667	19.44960485363665
135-139	23.889240158429647	23.5706558941308	28.041647002658515	24.498456944781037
140-144	22.760928552018562	27.231922629006423	26.513979554985106	23.49316926398991
145-149	22.74986607321817	27.094548056330638	27.27784249724554	22.877743373205657
150-154	22.800267733389052	26.933748133822967	27.2140785974852	23.051905535302787
155-159	22.69515280169338	26.731389109064242	27.28386220971019	23.289595879532197
160-164	22.563115301838415	26.740999827867633	27.414563369916827	23.281321500377125
165-169	22.533144444398552	26.605691595163165	27.247905065088695	23.61325889534959
170-174	22.522052694198187	26.74935257755403	27.2376375051416	23.490957223106186
175-179	22.694098668663187	26.38160923003174	27.10977421175898	23.814517889546096
180-184	22.780263751921378	26.364816813565383	26.998452691679866	23.85646674283337
185-189	22.65998299839656	25.97980366987727	27.59784792167792	23.76236541004825
190-194	22.668877531237964	26.4414022232705	27.0803156680927	23.809404577398837
195-199	22.68338274063822	26.10172290570649	27.492444190743125	23.72245016291216
200-204	22.944654857515363	25.988356539356683	26.93398606689245	24.1330025362355
205-209	23.254894160257436	25.41026505459028	27.192697634270836	24.14214315088145
210-214	23.22895274698529	26.10472970000987	26.50496008570309	24.16135746730175
215-219	23.526879699086667	26.56910673654831	26.000243693270914	23.903769871094113
220-224	23.911726214266622	26.47638014093017	26.513309714874044	23.09858392992916
225-229	23.56509454203283	26.969451464870115	26.138603901003236	23.326850092093814
230-234	23.76475406287763	27.05871360732196	26.025026287770697	23.151506042029713
235-239	24.01769708741403	27.135568750333594	26.018415697996094	22.828318464256284
240-244	24.157066242923825	26.558499073782205	26.016113814678715	23.26832086861525
245-249	23.301338771293516	27.475889644906857	26.043137976892822	23.179633606906805
250-252	22.50783940194204	28.03617918457967	26.152986869150656	23.302994544327635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.3333333333333333
11	1.0
12	1.9999999999999998
13	5.499999999999999
14	9.166666666666664
15	10.0
16	30.833333333333293
17	56.33333333333345
18	84.83333333333323
19	143.49999999999957
20	218.66666666666833
21	338.33333333333184
22	516.4999999999885
23	798.5000000000197
24	1169.1666666666836
25	1673.6666666665715
26	2315.833333333251
27	3214.833333333657
28	4310.500000000593
29	5696.333333332676
30	7309.166666664558
31	9195.33333333202
32	11310.500000002494
33	13676.333333340088
34	16407.000000011685
35	19218.000000002194
36	22180.666666658122
37	25172.499999980668
38	28138.333333303293
39	30821.999999960277
40	33210.49999995378
41	34640.33333329738
42	35870.49999997272
43	36256.33333330867
44	35895.9999999724
45	35026.83333329939
46	33637.33333328952
47	31918.83333329041
48	29923.999999964395
49	28305.666666636836
50	26808.499999975513
51	25570.16666664662
52	24518.166666650264
53	23407.66666665418
54	22117.49999999196
55	20916.833333329436
56	19936.999999999647
57	18785.333333337127
58	17527.333333341612
59	16070.666666677698
60	14637.166666675084
61	13014.166666672221
62	11359.833333335928
63	9650.333333332841
64	8023.9999999972215
65	6496.833333331934
66	5129.333333333176
67	3899.8333333339738
68	2861.5000000001696
69	2044.8333333331536
70	1429.999999999958
71	938.1666666667023
72	593.166666666663
73	371.3333333333301
74	219.00000000000173
75	120.49999999999942
76	57.00000000000012
77	23.166666666666657
78	9.83333333333333
79	5.499999999999999
80	1.6666666666666665
81	0.8333333333333333
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03389977992335387
2	1.208548303862883E-4
3	2.417096607725766E-4
4	2.417096607725766E-4
5	3.021370759657207E-4
6	2.417096607725766E-4
7	0.0
8	0.0
9	1.8128224557943244E-4
10-14	8.701547787812757E-4
15-19	0.0
20-24	1.0876934734765947E-4
25-29	0.002187472429991818
30-34	0.0024291820907643945
35-39	0.002900515929270919
40-44	0.011130729878577153
45-49	0.006163596349700704
50-54	0.001075607990437966
55-59	1.208548303862883E-4
60-64	1.0876934734765947E-4
65-69	0.0
70-74	8.580692957426469E-4
75-79	8.339013530955869E-4
80-84	0.002574241487055916
85-89	0.002054579542986578
90-94	0.005619962202430555
95-99	0.0030819873911665644
100-104	0.0020184903384416046
105-109	0.0013901596761580724
110-114	5.077947706327292E-4
115-119	8.948662731189912E-4
120-124	9.43540701805574E-4
125-129	0.0012131730699356665
130-134	0.002818186290616958
135-139	0.002429470940187033
140-144	0.001409093145308479
145-149	0.0016398928846262473
150-154	0.002089345769961573
155-159	0.0019678733739079673
160-164	0.0022715611237012017
165-169	0.002210836011846194
170-174	0.0028060866570579455
175-179	0.0038629883081435314
180-184	0.004555701837454259
185-189	0.0035601003875406208
190-194	0.0026861234377032065
195-199	0.0017511245807181482
200-204	0.001764793441881518
205-209	0.0023522910522626663
210-214	0.004447213771701548
215-219	0.011643403171667932
220-224	0.013522546901935108
225-229	0.014563641114943444
230-234	0.027222947180375316
235-239	0.07160146085713089
240-244	0.03087788289687691
245-249	2.3176364164416023E-4
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	11.0
80-84	21.0
85-89	19.0
90-94	34.0
95-99	51.0
100-104	148.0
105-109	270.0
110-114	287.0
115-119	421.0
120-124	1017.0
125-129	6150.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	2.0
160-164	6.0
165-169	12.0
170-174	19.0
175-179	59.0
180-184	184.0
185-189	424.0
190-194	682.0
195-199	1089.0
200-204	1990.0
205-209	2955.0
210-214	5647.0
215-219	8788.0
220-224	14018.0
225-229	21960.0
230-234	34120.0
235-239	45832.0
240-244	72945.0
245-249	200792.0
250-253	1234924.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.67882073935212
#Duplication Level	Percentage of total
1	86.23841664780558
2	8.74562266722156
3	1.7625939370329264
4	0.6996505948556309
5	0.4817696977563274
6	0.33047739887549965
7	0.3023813173654751
8	0.1843334899376326
9	0.1582954400284047
>10	1.071393461317209
>50	0.025065347803729106
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	6.042741519314415E-5	0.0	0.0	0.0	0.0	0.0
3	6.042741519314415E-5	0.0	0.0	0.0	0.0	0.0
4	6.042741519314415E-5	0.0	0.0	6.042741519314415E-5	0.0	0.0
5	6.042741519314415E-5	0.0	0.0	6.042741519314415E-5	0.0	0.0
6	6.042741519314415E-5	0.0	0.0	6.042741519314415E-5	0.0	0.0
7	6.042741519314415E-5	0.0	0.0	6.042741519314415E-5	0.0	0.0
8	6.042741519314415E-5	0.0	0.0	1.208548303862883E-4	0.0	0.0
9	6.042741519314415E-5	0.0	0.0	1.8128224557943244E-4	0.0	0.0
10-14	6.042741519314415E-5	0.0	0.0	2.7796610988846306E-4	8.45983812704018E-5	0.0
15-19	6.042741519314415E-5	0.0	0.0	6.767870501632144E-4	1.8128224557943244E-4	0.0
20-24	6.042741519314415E-5	0.0	0.0	0.002876344963193662	1.8128224557943244E-4	0.0
25-29	8.45983812704018E-5	2.417096607725766E-5	0.0	0.06570877128102495	2.2962417773394775E-4	0.0
30-34	2.2962417773394775E-4	6.042741519314415E-5	0.0	0.6305842485065365	7.130434992791009E-4	0.0
35-39	2.417096607725766E-4	6.042741519314415E-5	0.0	1.8919823696973435	8.459838127040181E-4	0.0
40-44	2.417096607725766E-4	6.042741519314415E-5	0.0	4.218123632074389	8.822402618199046E-4	0.0
45-49	2.417096607725766E-4	6.042741519314415E-5	2.417096607725766E-5	6.807970134354315	0.0010876934734765948	0.0
50-54	2.779661098884631E-4	6.042741519314415E-5	7.251289823177298E-5	9.521100649111293	0.001365659583365058	0.0
55-59	3.0213707596572076E-4	6.042741519314415E-5	1.208548303862883E-4	12.21856837785021	0.0017644805236398092	0.0
60-64	3.0213707596572076E-4	6.042741519314415E-5	1.208548303862883E-4	14.970686660889806	0.002501694988996168	2.417096607725766E-5
65-69	3.0213707596572076E-4	6.042741519314415E-5	1.208548303862883E-4	17.722575319751666	0.0028884304462322903	6.042741519314415E-5
70-74	3.0213707596572076E-4	6.042741519314415E-5	1.208548303862883E-4	20.508279160155617	0.0033235078356229283	6.042741519314415E-5
75-79	3.0213707596572076E-4	6.042741519314415E-5	1.208548303862883E-4	23.282876441647055	0.003553132013356876	6.042741519314415E-5
80-84	3.0213707596572076E-4	6.042741519314415E-5	1.208548303862883E-4	26.06954712069409	0.004181577131365575	6.042741519314415E-5
85-89	3.0213707596572076E-4	9.668386430903065E-5	1.208548303862883E-4	28.846561498793267	0.0047375093511425005	6.042741519314415E-5
90-94	3.0213707596572076E-4	1.208548303862883E-4	1.208548303862883E-4	31.573034386824894	0.008508180059194697	6.042741519314415E-5
95-99	3.5047900812023604E-4	1.208548303862883E-4	1.208548303862883E-4	34.25213218134509	0.059798970075135446	6.042741519314415E-5
100-104	4.2299190635200907E-4	1.8128224557943244E-4	1.208548303862883E-4	36.86891722531812	0.17542078630569746	8.459838127040181E-5
105-109	4.4716287242926666E-4	1.8128224557943244E-4	1.208548303862883E-4	39.440744272387455	0.388089031336449	1.691967625408036E-4
110-114	4.834193215451532E-4	1.8128224557943244E-4	1.208548303862883E-4	41.93928495031054	0.5856745935349917	1.8128224557943244E-4
115-119	4.834193215451532E-4	1.8128224557943244E-4	1.208548303862883E-4	43.39052183907212	0.7312925786674305	1.8128224557943244E-4
120-124	4.95504804583782E-4	2.054532116566901E-4	1.208548303862883E-4	43.42675411722194	0.749771282233494	1.8128224557943244E-4
125-129	5.438467367382973E-4	2.417096607725766E-4	1.208548303862883E-4	43.42732213492475	0.7498921370638804	1.8128224557943244E-4
130-134	5.438467367382973E-4	2.417096607725766E-4	1.208548303862883E-4	43.42826480260177	0.749964649962112	2.537951438112055E-4
135-139	5.438467367382973E-4	2.537951438112055E-4	1.208548303862883E-4	43.43019847988795	0.749964649962112	3.5047900812023604E-4
140-144	5.801031858541838E-4	3.0213707596572076E-4	1.208548303862883E-4	43.43245846521617	0.750061333826421	3.625644911588649E-4
145-149	6.647015671245856E-4	3.0213707596572076E-4	1.5711127950217478E-4	43.43634999075461	0.7503513854193482	3.625644911588649E-4
150-154	6.647015671245856E-4	3.0213707596572076E-4	2.417096607725766E-4	43.44383090475552	0.7505326676649275	4.2299190635200907E-4
155-159	7.251289823177298E-4	3.0213707596572076E-4	2.417096607725766E-4	43.45307629928006	0.7505810095970821	4.95504804583782E-4
160-164	7.251289823177298E-4	3.0213707596572076E-4	2.417096607725766E-4	43.46274468571097	0.7506897789444298	5.438467367382973E-4
165-169	7.492999483949875E-4	3.0213707596572076E-4	2.417096607725766E-4	43.47571240901142	0.7508106337748159	5.438467367382973E-4
170-174	9.18496710935791E-4	3.0213707596572076E-4	2.417096607725766E-4	43.4881604565412	0.7509073176391249	5.438467367382973E-4
175-179	9.910096091675642E-4	3.0213707596572076E-4	2.417096607725766E-4	43.50246966845894	0.750943574088241	5.921886688928127E-4
180-184	0.0010272660582834505	3.0213707596572076E-4	2.417096607725766E-4	43.522011894532405	0.7509919160203955	6.042741519314415E-4
185-189	0.0010272660582834505	3.0213707596572076E-4	2.417096607725766E-4	43.54517976551746	0.7510885998847044	6.767870501632144E-4
190-194	0.0011481208886697388	3.0213707596572076E-4	2.417096607725766E-4	43.56661941242798	0.7515236772740951	7.251289823177298E-4
195-199	0.001208548303862883	3.0213707596572076E-4	2.417096607725766E-4	43.58959391568442	0.7531914739334259	7.251289823177298E-4
200-204	0.001208548303862883	3.0213707596572076E-4	2.417096607725766E-4	43.613873651109024	0.7640442377021145	7.251289823177298E-4
205-209	0.001208548303862883	3.0213707596572076E-4	2.417096607725766E-4	43.63561543509552	0.7945359114085752	7.251289823177298E-4
210-214	0.0012327192699401405	3.2630804204297845E-4	2.417096607725766E-4	43.65897667380919	0.8363516827222309	7.251289823177298E-4
215-219	0.0013173176512105427	3.625644911588649E-4	2.417096607725766E-4	43.688126858898364	0.8908088692942924	7.251289823177298E-4
220-224	0.0013294031342491713	3.625644911588649E-4	2.417096607725766E-4	43.721361937254585	0.9637689303984945	7.251289823177298E-4
225-229	0.0013294031342491713	3.625644911588649E-4	2.417096607725766E-4	43.7519019528932	1.0510744598695492	7.251289823177298E-4
230-234	0.0013294031342491713	3.7464997419749374E-4	2.6588062684983427E-4	43.78076208638945	1.1282040126220785	7.251289823177298E-4
235-239	0.0013777450664036865	4.2299190635200907E-4	3.0213707596572076E-4	43.8173206725813	1.186202245724458	7.251289823177298E-4
240-241	0.0013898305494423153	4.22991906352009E-4	3.0213707596572076E-4	43.89057682801995	1.187579990790862	7.251289823177298E-4
>>END_MODULE
