##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665033
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2355482
Total Bases	583.5 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.476476576768576	28.0	18.0	32.0	18.0	33.0
2	30.192900221695602	31.0	29.0	33.0	27.0	33.0
3	30.73831088499084	33.0	31.0	33.0	27.0	33.0
4	31.334655072719723	33.0	31.0	33.0	29.0	33.0
5	31.269771961747107	33.0	31.0	33.0	28.0	34.0
6	33.93159149592313	37.0	33.0	38.0	26.0	38.0
7	34.766890598187544	37.0	34.0	38.0	29.0	38.0
8	34.85049514282002	38.0	34.0	38.0	29.0	38.0
9	36.09128365234801	38.0	37.0	38.0	33.0	38.0
10-14	36.47252494393929	38.0	37.6	38.0	33.6	38.0
15-19	36.57712383282912	38.0	38.0	38.0	34.0	38.0
20-24	36.71338978603954	38.0	38.0	38.0	34.0	38.0
25-29	36.70452366012561	38.0	38.0	38.0	34.0	38.0
30-34	36.687090200646836	38.0	38.0	38.0	34.0	38.0
35-39	36.66540996704708	38.0	38.0	38.0	34.0	38.0
40-44	36.63598320853227	38.0	38.0	38.0	34.0	38.0
45-49	36.62334961591725	38.0	38.0	38.0	34.0	38.0
50-54	36.57193101029853	38.0	38.0	38.0	34.0	38.0
55-59	36.494569009654924	38.0	38.0	38.0	34.0	38.0
60-64	36.44167028234561	38.0	38.0	38.0	34.0	38.0
65-69	36.371847375611445	38.0	37.2	38.0	33.6	38.0
70-74	36.29497699409293	38.0	37.0	38.0	33.0	38.0
75-79	36.16381357209346	38.0	37.0	38.0	33.0	38.0
80-84	36.00746174168721	38.0	37.0	38.0	32.2	38.0
85-89	35.84990454680336	38.0	37.0	38.0	31.0	38.0
90-94	35.724565158682104	38.0	36.6	38.0	31.0	38.0
95-99	35.59713812458065	38.0	36.0	38.0	29.6	38.0
100-104	35.45661489053022	38.0	36.0	38.0	29.0	38.0
105-109	35.26453029659109	38.0	35.4	38.0	28.4	38.0
110-114	35.09179959910845	38.0	35.0	38.0	28.0	38.0
115-119	34.91508926261896	38.0	34.6	38.0	27.6	38.0
120-124	35.00241982999209	38.0	34.0	38.0	28.8	38.0
125-129	33.77882833298899	34.2	33.2	35.6	31.2	35.6
130-134	35.39898249588578	36.2	36.0	37.2	33.2	38.0
135-139	36.64662352262284	38.0	38.0	38.0	34.0	38.0
140-144	36.605556348714444	38.0	38.0	38.0	34.0	38.0
145-149	36.59833670307123	38.0	38.0	38.0	34.0	38.0
150-154	36.588255595504585	38.0	38.0	38.0	34.0	38.0
155-159	36.58644669045851	38.0	38.0	38.0	34.0	38.0
160-164	36.57631168248115	38.0	38.0	38.0	34.0	38.0
165-169	36.53645546126601	38.0	38.0	38.0	34.0	38.0
170-174	36.51383986946205	38.0	38.0	38.0	34.0	38.0
175-179	36.470643089468695	38.0	38.0	38.0	34.0	38.0
180-184	36.406628329271356	38.0	38.0	38.0	34.0	38.0
185-189	36.3219777074577	38.0	38.0	38.0	33.2	38.0
190-194	36.211274867018034	38.0	38.0	38.0	33.0	38.0
195-199	36.0765783790135	38.0	37.0	38.0	32.8	38.0
200-204	35.883519038764845	38.0	37.0	38.0	31.0	38.0
205-209	35.705542092573985	38.0	37.0	38.0	30.2	38.0
210-214	35.5333640782746	38.0	37.0	38.0	29.0	38.0
215-219	35.29792807793107	38.0	36.2	38.0	28.0	38.0
220-224	35.0945099864764	38.0	36.0	38.0	26.6	38.0
225-229	34.83218175258089	38.0	35.0	38.0	24.8	38.0
230-234	34.59239583639825	38.0	35.0	38.0	23.4	38.0
235-239	34.410894785157716	38.0	34.4	38.0	23.0	38.0
240-244	34.317894438013624	38.0	34.0	38.0	23.0	38.0
245-249	34.63046825016465	38.0	34.0	38.0	25.2	38.0
250-252	35.8165385713124	38.0	34.666666666666664	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	2.0
18	15.0
19	23.0
20	107.0
21	399.0
22	1268.0
23	2679.0
24	5131.0
25	8502.0
26	13023.0
27	19034.0
28	26328.0
29	34842.0
30	46054.0
31	60445.0
32	80111.0
33	110630.0
34	165736.0
35	259772.0
36	478969.0
37	1042410.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.4802120118832	20.62711688046938	15.374726331816435	23.517944775830983
2	15.471395165987682	22.827275675914336	38.75136065916189	22.949968498936098
3	17.39047002853778	28.751537907019987	31.51072777708436	22.347264287357874
4	13.200171345336848	20.371627487765746	37.91355211704466	28.514649049852746
5	15.49453633225485	34.72887989610562	34.809670600631975	14.966913171007556
6	29.016665010948966	37.08149612349786	20.207822289696256	13.694016575856919
7	24.316636679881228	30.69074609782626	26.419178749826997	18.573438472465508
8	23.225711956798616	34.80861650279349	22.332475758656408	19.63319578175149
9	23.178969891487085	17.424304175794315	21.131234398084466	38.26549153463413
10-14	23.734562342827793	23.586446762068928	28.985550070139233	23.693440824964046
15-19	23.108947231852714	28.327234651291693	26.172677383682384	22.391140733173206
20-24	22.909198264200338	27.14431950831916	27.04923057694587	22.897251650534635
25-29	23.134878247180556	27.066741161266656	26.742035842318252	23.056344749234533
30-34	22.970290707626763	26.981624270106213	27.015130097029346	23.032954925237682
35-39	22.843067511271524	26.657502496810316	27.268318843823558	23.231111148094605
40-44	22.550082332298096	26.23914333309641	27.701517120456003	23.509257214149486
45-49	21.975451800118677	26.167036380748783	28.208666492084177	23.648845327048367
50-54	21.539758034626768	26.280927597612525	28.025664615122047	24.153649752638657
55-59	21.206445732315395	26.015595116241652	27.88148153179754	24.896477619645413
60-64	21.293906754647587	26.043663959165187	27.275591000460032	25.386838285727194
65-69	21.356834821917552	26.184611047760075	26.657253165169593	25.80130096515278
70-74	21.521293856656825	26.437269201465835	26.161773517686584	25.879663424190756
75-79	21.795341407855048	26.545720357447195	25.82563971412104	25.833298520576715
80-84	21.791156275953643	26.52341125381007	25.784310054328746	25.90112241590754
85-89	21.841033629715646	26.058142945184414	26.03791691977502	26.06290650532491
90-94	21.859950444549533	25.55286663395125	26.25787566777112	26.3293072537281
95-99	21.71270686329988	25.212450757346744	26.67188955243956	26.40295282691382
100-104	21.58683550529731	25.4302699954093	26.77341958349447	26.209474915798925
105-109	21.276681394757507	26.592569303642072	26.450579365487826	25.680169936112595
110-114	20.99393007224422	27.77707561341843	26.00746853723252	25.22152577710483
115-119	20.842016781306587	28.208438094017936	25.937825124858993	25.01171999981649
120-124	20.949297428963114	28.402814193694244	25.825556026778695	24.822332350563954
125-129	22.774120566951023	26.042364820732384	27.71464914755442	23.468865464762175
130-134	22.011834612934255	30.694785513670485	27.30127257910574	19.992107294289518
135-139	23.95643709033564	23.464931760258658	27.950333604829236	24.628297544576466
140-144	22.83874899248256	27.17205713431776	26.519038788181792	23.470155085017886
145-149	22.761151890138482	27.0167793545947	27.225524241020366	22.99654451424645
150-154	22.827480568319743	26.842456365119727	27.17477214470334	23.155290921857187
155-159	22.6391874346359	26.691011414143773	27.305657298350283	23.364143852870043
160-164	22.509525356937463	26.606555203298093	27.488204818064766	23.395714621699682
165-169	22.478916000243824	26.517797061324693	27.31104977103768	23.692237167393795
170-174	22.4965622829519	26.63323620065666	27.28768485102998	23.582516665361457
175-179	22.566228834542137	26.30985423227081	27.16999805752463	23.953918875662424
180-184	22.71410711473339	26.23068124357566	27.07302938381591	23.982182257875035
185-189	22.626044387572822	25.956779846101146	27.551514112924945	23.865661653401087
190-194	22.60788788487397	26.27945468418009	27.221183422234844	23.891474008711093
195-199	22.510503054640534	26.215403590122595	27.482830376822864	23.79126297841401
200-204	22.913253086761305	25.87819187743711	27.03875622666953	24.169798809132054
205-209	23.18980242922912	25.50785794105963	27.14670790155268	24.155631728158568
210-214	23.56939364429503	26.014846810675557	26.312214727516082	24.103544817513328
215-219	23.71831346970983	26.611313161883267	25.94995244407731	23.720420924329595
220-224	23.990200874935542	26.43032419070549	26.43532267985781	23.14415225450116
225-229	23.656020896557315	27.060671042866417	26.198065274775022	23.085242785801242
230-234	23.801374085798336	27.14515501264684	26.18390969333433	22.869561208220496
235-239	24.14533808105791	27.25549609493298	26.028375256005354	22.570790568003748
240-244	23.773501454131242	26.95321253618697	26.27315124998646	23.00013475969533
245-249	23.56736108091756	27.492794883147386	26.083635489979585	22.856208545955468
250-252	22.6672786935543	28.102197304365127	26.21913598742069	23.011388014659882
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.8333333333333333
14	4.5
15	5.499999999999999
16	8.166666666666664
17	11.333333333333336
18	14.666666666666675
19	28.8333333333333
20	56.83333333333345
21	78.99999999999997
22	121.16666666666606
23	211.83333333333488
24	316.833333333333
25	488.99999999999
26	735.6666666666795
27	1104.0000000000327
28	1600.3333333332569
29	2297.1666666665737
30	3263.8333333336777
31	4651.333333333638
32	6472.166666665349
33	8960.49999999813
34	12100.333333337101
35	16276.833333344634
36	21309.333333328406
37	27526.999999972828
38	34920.999999964464
39	43028.8333333562
40	51664.833333418464
41	59560.500000141874
42	67036.50000018062
43	73186.00000009249
44	76582.66666671104
45	76934.83333337266
46	74172.0000000795
47	68725.33333349187
48	61611.00000015429
49	53561.83333342969
50	45984.83333337561
51	39186.83333332779
52	33139.66666661987
53	28282.833333303763
54	24466.333333317267
55	21451.166666661236
56	19280.333333335544
57	17366.16666667565
58	15825.166666677247
59	14746.000000008671
60	13520.666666673142
61	12216.000000004124
62	10896.166666668438
63	9276.666666665536
64	7738.99999999749
65	6307.666666665452
66	4908.166666666715
67	3760.666666667242
68	2700.166666666763
69	1890.999999999855
70	1307.3333333333194
71	845.3333333333583
72	582.9999999999952
73	402.1666666666617
74	258.00000000000273
75	156.49999999999997
76	92.16666666666644
77	54.5000000000001
78	39.33333333333332
79	24.833333333333314
80	14.000000000000005
81	7.833333333333331
82	4.5
83	1.9999999999999998
84	0.3333333333333333
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03808137782415658
2	2.547249352786394E-4
3	3.396332470381858E-4
4	2.1227077939886614E-4
5	5.51904026437052E-4
6	3.396332470381858E-4
7	0.0
8	8.490831175954645E-5
9	8.490831175954645E-5
10-14	0.001027390572290512
15-19	1.698166235190929E-5
20-24	1.1887163646336504E-4
25-29	0.0022925244175077543
30-34	0.0030057542362879443
35-39	0.003260479171566584
40-44	0.011471112918714726
45-49	0.006300196732558347
50-54	8.915372734752377E-4
55-59	4.2454155879773225E-5
60-64	6.792664940763716E-5
65-69	0.0
70-74	7.896472993637819E-4
75-79	6.792686857475506E-4
80-84	0.002190661414173834
85-89	0.0017746281665756002
90-94	0.005298495948900761
95-99	0.0024285319558292493
100-104	0.001537003703839257
105-109	0.0011804754122682988
110-114	6.115587664655827E-4
115-119	0.0012828541278465237
120-124	0.0010963608553620418
125-129	0.0016384218106711436
130-134	0.0031973327064053515
135-139	0.0027955288636217915
140-144	0.0015559212635448503
145-149	0.001684156532518327
150-154	0.0020517648297928566
155-159	0.002162904865014219
160-164	0.0023509875686616604
165-169	0.0023167989122842834
170-174	0.0031375343750232433
175-179	0.004300277705607776
180-184	0.00518109730511123
185-189	0.003865023131137328
190-194	0.002908175222689241
195-199	0.0016773113178806178
200-204	0.001627357141168607
205-209	0.0021873958306348304
210-214	0.0045576162669407885
215-219	0.013359822686567937
220-224	0.014428338787129505
225-229	0.016537356773580137
230-234	0.031227590898550702
235-239	0.08033008328190017
240-244	0.03308025638813762
245-249	2.653623359397358E-4
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	19.0
80-84	26.0
85-89	32.0
90-94	44.0
95-99	78.0
100-104	171.0
105-109	320.0
110-114	418.0
115-119	676.0
120-124	1871.0
125-129	12377.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	2.0
155-159	4.0
160-164	5.0
165-169	13.0
170-174	19.0
175-179	69.0
180-184	224.0
185-189	538.0
190-194	948.0
195-199	1510.0
200-204	2915.0
205-209	4602.0
210-214	8008.0
215-219	12583.0
220-224	19383.0
225-229	31278.0
230-234	49170.0
235-239	65741.0
240-244	104398.0
245-249	288011.0
250-253	1750029.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.1636226502535
#Duplication Level	Percentage of total
1	84.22095831796634
2	11.477473580237344
3	2.5433183527551946
4	0.8847228924502463
5	0.34453145849086153
6	0.15267668798173523
7	0.11634672260921763
8	0.0634211909693437
9	0.04472410525603847
>10	0.15182669128366869
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.2454155879773225E-5	0.0	0.0
3	0.0	0.0	0.0	4.2454155879773225E-5	0.0	0.0
4	4.2454155879773225E-5	0.0	0.0	8.490831175954645E-5	0.0	0.0
5	8.490831175954645E-5	0.0	0.0	1.698166235190929E-4	0.0	0.0
6	8.490831175954645E-5	0.0	0.0	1.698166235190929E-4	0.0	0.0
7	8.490831175954645E-5	0.0	0.0	1.698166235190929E-4	0.0	0.0
8	8.490831175954645E-5	0.0	0.0	2.1227077939886614E-4	0.0	0.0
9	8.490831175954645E-5	0.0	0.0	2.1227077939886614E-4	0.0	0.0
10-14	8.490831175954645E-5	0.0	3.396332470381858E-5	2.7170659763054865E-4	4.2454155879773225E-5	4.2454155879773225E-5
15-19	9.33991429355011E-5	6.792664940763716E-5	8.490831175954645E-5	7.217206499561448E-4	6.792664940763716E-5	5.943581823168252E-5
20-24	1.273624676393197E-4	1.698166235190929E-4	8.490831175954645E-5	0.004398250549144507	9.33991429355011E-5	8.490831175954645E-5
25-29	2.037799482229115E-4	1.698166235190929E-4	1.1038080528741039E-4	0.0674681445241356	1.273624676393197E-4	8.490831175954645E-5
30-34	2.547249352786394E-4	1.698166235190929E-4	1.273624676393197E-4	0.605999111859059	2.971790911584126E-4	8.490831175954645E-5
35-39	2.6321576645459404E-4	1.698166235190929E-4	1.528349611671836E-4	1.8726867791815007	3.396332470381858E-4	8.490831175954645E-5
40-44	2.971790911584126E-4	2.1227077939886614E-4	1.698166235190929E-4	4.310158175693976	4.330323899736869E-4	8.490831175954645E-5
45-49	2.971790911584126E-4	2.1227077939886614E-4	1.7830745469504756E-4	7.096585751875837	6.537940005485077E-4	1.273624676393197E-4
50-54	3.396332470381858E-4	2.547249352786394E-4	2.1227077939886614E-4	10.043974014660268	9.339914293550109E-4	1.273624676393197E-4
55-59	3.396332470381858E-4	2.886882599824579E-4	2.1227077939886614E-4	12.969693676283663	0.0011802255334576958	1.273624676393197E-4
60-64	3.396332470381858E-4	3.396332470381858E-4	2.1227077939886614E-4	15.958092653647958	0.0015453312740237456	1.273624676393197E-4
65-69	3.396332470381858E-4	3.396332470381858E-4	2.1227077939886614E-4	18.885867096415936	0.0018849645210619313	1.3585329881527432E-4
70-74	4.160507276217776E-4	3.396332470381858E-4	2.1227077939886614E-4	21.80764701237369	0.0022585610928039356	1.698166235190929E-4
75-79	4.6699571467750543E-4	3.396332470381858E-4	2.547249352786394E-4	24.664675849783613	0.0026491393268978495	1.698166235190929E-4
80-84	4.6699571467750543E-4	3.396332470381858E-4	2.547249352786394E-4	27.51327329183581	0.002971790911584126	1.698166235190929E-4
85-89	5.51904026437052E-4	3.396332470381858E-4	2.7170659763054865E-4	30.281759741742878	0.0036425665744845423	1.698166235190929E-4
90-94	6.113398446687345E-4	3.396332470381858E-4	3.2265158468627647E-4	32.978243943277846	0.008057798785980959	1.698166235190929E-4
95-99	6.368123381965984E-4	3.396332470381858E-4	3.396332470381858E-4	35.599889958827966	0.06014055721928675	1.698166235190929E-4
100-104	6.368123381965984E-4	3.8208740291795906E-4	3.396332470381858E-4	38.15515465624445	0.18728226324803163	1.698166235190929E-4
105-109	7.217206499561448E-4	3.8208740291795906E-4	3.396332470381858E-4	40.64982878238933	0.4190564818580656	1.698166235190929E-4
110-114	7.471931434840089E-4	3.9906906526986834E-4	3.396332470381858E-4	43.02622563025317	0.6303338340093451	1.698166235190929E-4
115-119	7.641748058359181E-4	4.6699571467750543E-4	3.396332470381858E-4	44.398751508183885	0.7760704603134305	1.698166235190929E-4
120-124	7.981381305397367E-4	4.6699571467750543E-4	3.396332470381858E-4	44.43032041849608	0.7937144074970643	1.698166235190929E-4
125-129	8.405922864195099E-4	4.839773770294148E-4	3.396332470381858E-4	44.43062608841842	0.7937993158088239	1.698166235190929E-4
130-134	8.490831175954646E-4	5.094498705572788E-4	3.396332470381858E-4	44.43189971309482	0.7938078066399998	1.7830745469504756E-4
135-139	8.490831175954646E-4	5.264315329091881E-4	3.396332470381858E-4	44.43361486099236	0.7938417699647037	2.547249352786394E-4
140-144	8.490831175954646E-4	5.51904026437052E-4	3.396332470381858E-4	44.43651872525454	0.7939181874452872	2.547249352786394E-4
145-149	8.490831175954646E-4	5.51904026437052E-4	3.396332470381858E-4	44.44119717323248	0.7940370590817505	2.886882599824579E-4
150-154	8.915372734752379E-4	5.51904026437052E-4	3.396332470381858E-4	44.44810021897853	0.7940880040688063	2.971790911584126E-4
155-159	9.339914293550109E-4	5.51904026437052E-4	3.396332470381858E-4	44.45752504158385	0.7941559307182139	2.971790911584126E-4
160-164	9.764455852347842E-4	5.51904026437052E-4	3.396332470381858E-4	44.46939522356783	0.7942408390299734	3.2265158468627647E-4
165-169	0.0010188997411145575	5.51904026437052E-4	3.396332470381858E-4	44.48393152654107	0.7944021648223165	3.396332470381858E-4
170-174	0.001103808052874104	5.51904026437052E-4	3.396332470381858E-4	44.49662531914912	0.7944870731340762	3.9057823409391373E-4
175-179	0.001103808052874104	5.51904026437052E-4	3.396332470381858E-4	44.51193428775936	0.7946229264328915	4.245415587977323E-4
180-184	0.0011462622087538774	5.51904026437052E-4	3.396332470381858E-4	44.53333118232276	0.7947927430564106	4.245415587977323E-4
185-189	0.0011887163646336503	6.368123381965983E-4	3.396332470381858E-4	44.55692720216075	0.7951918121216803	4.330323899736869E-4
190-194	0.0011887163646336503	7.132298187801901E-4	3.396332470381858E-4	44.579809992179946	0.7957522069792933	4.6699571467750543E-4
195-199	0.0011887163646336503	7.302114811320995E-4	3.396332470381858E-4	44.603074869602054	0.7984013463061912	5.349223640851426E-4
200-204	0.0011887163646336503	7.641748058359181E-4	3.396332470381858E-4	44.628309619857	0.8082507104702987	5.688856887889613E-4
205-209	0.0012141888581615142	7.641748058359181E-4	3.396332470381858E-4	44.651022593252684	0.8342581263622477	5.943581823168252E-4
210-214	0.0012736246763931968	7.641748058359181E-4	3.651057405660498E-4	44.67244496030961	0.8685950476378084	5.943581823168252E-4
215-219	0.0012736246763931968	7.811564681878273E-4	3.8208740291795906E-4	44.70351291158243	0.9167635328989991	5.943581823168252E-4
220-224	0.0012736246763931968	8.066289617156913E-4	3.8208740291795906E-4	44.73728943800038	0.9866515643082817	6.707756629004168E-4
225-229	0.0012821155075691515	8.236106240676007E-4	3.8208740291795906E-4	44.76710074625915	1.0667710472845897	6.962481564282809E-4
230-234	0.0013245696634489248	8.490831175954646E-4	3.8208740291795906E-4	44.79569786565976	1.137830813396154	7.217206499561448E-4
235-239	0.0013585329881527432	8.490831175954646E-4	3.8208740291795906E-4	44.83264996293752	1.1902192417517945	7.217206499561448E-4
240-241	0.0013585329881527432	8.490831175954646E-4	3.8208740291795906E-4	44.90603197137571	1.1919216534025732	7.217206499561448E-4
>>END_MODULE
