##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665038
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5204601
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	153-252
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.05666639959528	32.0	25.0	33.0	18.0	33.0
2	27.92289360894332	29.0	25.0	33.0	18.0	33.0
3	29.721677415809587	31.0	29.0	33.0	25.0	33.0
4	30.248523758113254	31.0	29.0	33.0	27.0	33.0
5	31.42163501102198	33.0	32.0	33.0	28.0	33.0
6	34.568825737073794	37.0	34.0	38.0	29.0	38.0
7	35.96648484677308	38.0	36.0	38.0	31.0	38.0
8	36.41920523782707	38.0	37.0	38.0	34.0	38.0
9	36.60625185292782	38.0	37.0	38.0	34.0	38.0
10-14	36.75656289502308	38.0	38.0	38.0	34.0	38.0
15-19	36.76416459205999	38.0	38.0	38.0	34.0	38.0
20-24	36.76522415455094	38.0	38.0	38.0	34.0	38.0
25-29	36.68821506201916	38.0	38.0	38.0	34.0	38.0
30-34	36.65636309104194	38.0	38.0	38.0	34.0	38.0
35-39	36.62883221979937	38.0	38.0	38.0	34.0	38.0
40-44	36.60244683502155	38.0	38.0	38.0	34.0	38.0
45-49	36.55603712945526	38.0	38.0	38.0	34.0	38.0
50-54	36.46884616130997	38.0	38.0	38.0	34.0	38.0
55-59	36.3847982583103	38.0	37.2	38.0	33.8	38.0
60-64	36.32105239191247	38.0	37.0	38.0	33.0	38.0
65-69	36.28280208223455	38.0	37.0	38.0	33.0	38.0
70-74	36.18499654440369	38.0	37.0	38.0	33.0	38.0
75-79	36.022911727527244	38.0	37.0	38.0	31.8	38.0
80-84	35.80158832540669	38.0	37.0	38.0	31.0	38.0
85-89	35.653916986143614	38.0	36.2	38.0	29.8	38.0
90-94	35.53741556749499	38.0	36.0	38.0	29.0	38.0
95-99	35.42631471653639	38.0	36.0	38.0	29.0	38.0
100-104	35.22139787468818	38.0	35.4	38.0	28.0	38.0
105-109	35.01166387202401	38.0	35.0	38.0	27.0	38.0
110-114	34.85634264759201	38.0	35.0	38.0	27.0	38.0
115-119	34.71074432026586	38.0	34.0	38.0	26.2	38.0
120-124	34.90605835106284	38.0	34.0	38.0	28.4	38.0
125-129	33.74489975312228	34.4	33.2	35.6	31.2	37.0
130-134	35.25475228552583	36.2	36.0	37.2	32.8	38.0
135-139	36.39588890675769	38.0	38.0	38.0	33.6	38.0
140-144	36.3378032244931	38.0	38.0	38.0	33.0	38.0
145-149	36.32312525013925	38.0	38.0	38.0	33.0	38.0
150-154	36.304380692991074	38.0	38.0	38.0	33.0	38.0
155-159	36.304222059280114	38.0	38.0	38.0	33.0	38.0
160-164	36.29018579759107	38.0	38.0	38.0	33.0	38.0
165-169	36.259854843524785	38.0	37.2	38.0	33.0	38.0
170-174	36.20558104402261	38.0	37.0	38.0	33.0	38.0
175-179	36.152543586978574	38.0	37.0	38.0	33.0	38.0
180-184	36.06130510026257	38.0	37.0	38.0	32.2	38.0
185-189	35.94425210061682	38.0	37.0	38.0	31.0	38.0
190-194	35.85420275422841	38.0	37.0	38.0	31.0	38.0
195-199	35.728316472908745	38.0	37.0	38.0	30.0	38.0
200-204	35.59793277617589	38.0	37.0	38.0	29.0	38.0
205-209	35.422828428782886	38.0	36.2	38.0	28.8	38.0
210-214	35.23762119001091	38.0	36.0	38.0	27.6	38.0
215-219	34.971927657473444	38.0	35.2	38.0	26.2	38.0
220-224	34.76779897736914	38.0	35.0	38.0	25.2	38.0
225-229	34.54564778095281	38.0	34.6	38.0	24.0	38.0
230-234	34.388553242719965	38.0	34.0	38.0	23.0	38.0
235-239	34.148144482224474	38.0	34.0	38.0	23.0	38.0
240-244	34.03103221962302	38.0	33.8	38.0	23.0	38.0
245-249	34.34179239244676	38.0	33.4	38.0	24.4	38.0
250-252	35.591623552470786	37.666666666666664	34.333333333333336	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	26.0
20	183.0
21	825.0
22	2858.0
23	6814.0
24	13534.0
25	23664.0
26	35940.0
27	52507.0
28	71190.0
29	92780.0
30	119100.0
31	151050.0
32	194842.0
33	256779.0
34	355405.0
35	550423.0
36	1099732.0
37	2176944.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.82436328914457	26.45106520736561	14.134218761744618	23.5903527417452
2	15.103847537976495	24.218148518973884	38.898966510593226	21.7790374324564
3	16.986143606397494	30.01780539949172	31.917124867016707	21.078926127094086
4	13.17488122528509	20.141178929950634	41.872105085481095	24.81183475928318
5	13.135127937761224	38.29027431689768	36.05300387099799	12.521593874343107
6	24.4694261865607	43.42344398734889	20.109053508616704	11.998076317473712
7	22.048645035421544	33.49820668289462	27.830452324779554	16.622695956904284
8	21.20971425090992	37.716032410553666	22.329338982949896	18.744914355586527
9	22.378737582381437	17.77390812475346	23.777115671306984	36.07023862155812
10-14	22.228120849225522	24.932624037846512	30.30909766185727	22.5301574510707
15-19	21.4764474740715	30.268268403283937	27.3607294776295	20.894554645015052
20-24	21.17462063781765	29.00573743450734	28.64283475877355	21.17680716890146
25-29	21.32514168744206	28.754484725916207	28.545037400319995	21.375336186321732
30-34	21.143434434263067	28.619738573619763	28.839859193817162	21.396967798300004
35-39	21.064385146911356	28.055153507444665	29.06369575688895	21.816765588755025
40-44	20.68560106720957	27.25128785088425	29.8350171319569	22.228093949949283
45-49	19.83829157150364	27.098631566076108	30.475436121881334	22.587640740538927
50-54	19.12475438887464	27.462225112674084	30.12733350743677	23.285686991014508
55-59	18.894949731961823	26.7992217072553	29.674584002927606	24.63124455785527
60-64	19.25256007334297	26.7127754055266	28.45653469535995	25.578129825770485
65-69	19.57246284917167	26.749038324821406	27.158829158068272	26.51966966793865
70-74	20.33994044030547	26.577017754805738	26.110968083500865	26.97207372138793
75-79	21.100375062267624	26.06689677536532	25.71835949668776	27.1143686656793
80-84	21.25545741277401	25.68081334837473	25.810505439850985	27.253223799000274
85-89	21.473461636954838	24.757590020096178	26.343034753756466	27.42591358919252
90-94	21.63309528097903	23.863105402524035	26.613895318423253	27.889903998073684
95-99	21.435194155189038	23.15369289856729	27.349297808172835	28.061815138070838
100-104	21.38647044442291	23.566329186480637	27.450388445122766	27.59681192397369
105-109	20.86697021212201	25.815000410599882	26.69736936708171	26.6206600101964
110-114	20.33855813057674	27.86358961447234	26.011058444531855	25.786793810419066
115-119	20.12290871403338	28.631036179561853	25.95463975207671	25.291415354328063
120-124	20.21237018106411	28.998833261362485	25.959767519227594	24.829029038345816
125-129	21.391164199849477	27.05625158075921	29.01611240476046	22.536471814630854
130-134	19.66908706057532	32.828678352692066	29.12558137210832	18.376653214624298
135-139	22.522803389539725	24.76685461572826	29.525312554093386	23.185029440638626
140-144	21.227265210416988	28.939402679653035	27.952861743494285	21.880470366435695
145-149	21.018258414821638	28.61056843220978	29.0848694717129	21.28630368125568
150-154	20.988129403434066	28.30780346404281	29.12875874201461	21.575308390508518
155-159	20.75604487505551	27.976031688717878	29.35785687231678	21.910066563909826
160-164	20.61482814721377	27.709548094451293	29.588538766085193	22.087084992249743
165-169	20.48300420479891	27.63021305938918	29.286705362531023	22.600077373280882
170-174	20.437950930408597	27.53089447867172	29.234541307731238	22.796613283188446
175-179	20.527392622425157	26.914975382794452	29.225428357670996	23.33220363710939
180-184	20.714755329959463	26.4359809355637	29.112739215819733	23.736524518657113
185-189	20.184245480561835	25.79898327414597	30.05432384832738	23.962447396964812
190-194	20.08920238682786	25.80436283350477	29.760636866681118	24.345797912986257
195-199	19.99484540521389	25.506878739080317	30.13842132230734	24.359854533398455
200-204	20.666768094668324	24.698138883342313	29.4541994115535	25.180893610435866
205-209	21.15490434362591	24.083853157867896	29.536829363807897	25.2244131346983
210-214	21.276779402342584	24.574180626824717	28.815875805974244	25.333164164858456
215-219	20.753004805668205	24.71713811314584	28.76427394720644	25.765583133979515
220-224	21.10398251337345	24.53078918213467	29.574006806897273	24.79122149759461
225-229	20.675251628999362	25.300048533871006	29.141384173384594	24.88331566374504
230-234	21.269961767735122	25.700433307043667	28.733168465025933	24.29643646019528
235-239	21.557963996234527	25.281392204757964	28.979390564400266	24.18125323460724
240-244	21.48682823471834	24.975798544701032	28.962673490402207	24.57469973017842
245-249	21.372976677690108	26.185057027955377	28.51758980032497	23.924376494029545
250-252	19.487762993692094	27.42643945898279	28.715574791542288	24.370222755782834
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.3333333333333333
10	0.3333333333333333
11	0.3333333333333333
12	5.333333333333332
13	18.333333333333336
14	35.33333333333331
15	72.00000000000006
16	106.66666666666623
17	124.9999999999993
18	167.66666666666706
19	218.00000000000182
20	300.3333333333337
21	492.66666666665617
22	902.833333333366
23	1700.1666666665594
24	2902.6666666668693
25	4527.66666666702
26	7030.166666664743
27	10218.666666667372
28	13865.33333334067
29	18696.500000003507
30	24260.166666649944
31	30683.833333293245
32	38381.49999999315
33	46438.50000005176
34	56538.6666667919
35	68153.5000001526
36	81486.66666662533
37	97145.16666639753
38	111970.49999951513
39	126660.4999993014
40	140952.49999952482
41	150024.33333312205
42	151082.83333315284
43	147048.49999970224
44	138026.99999944004
45	126085.33333264383
46	113383.33333282865
47	100632.99999968088
48	89594.83333317467
49	80583.16666663875
50	71570.66666676912
51	64113.33333351317
52	56836.00000012697
53	50525.33333341457
54	45356.33333337709
55	40451.833333341514
56	36656.66666664736
57	33237.16666662247
58	31041.16666662533
59	29194.499999965374
60	27126.66666663955
61	24919.666666647572
62	22375.999999990127
63	19574.166666666966
64	16343.833333345165
65	13255.166666672885
66	10646.666666668145
67	8446.666666664149
68	6680.499999998394
69	5074.333333333188
70	3613.3333333338583
71	2606.6666666667343
72	1871.9999999998536
73	1319.4999999999793
74	892.0000000000315
75	615.6666666666667
76	413.6666666666606
77	281.6666666666681
78	162.8333333333336
79	96.33333333333304
80	55.33333333333345
81	31.166666666666625
82	15.666666666666677
83	8.999999999999998
84	6.666666666666664
85	2.6666666666666665
86	1.3333333333333333
87	0.3333333333333333
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.052837864036071165
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	1.9213768740389514E-4
25-29	8.953616233021513E-4
30-34	0.0
35-39	0.0
40-44	0.0
45-49	4.5728769602127047E-4
50-54	6.186833534405423E-4
55-59	0.0015601580217196286
60-64	0.002328708771335209
65-69	0.0027091413923949212
70-74	0.009176495950410032
75-79	0.008680780716907982
80-84	0.0023094950025948197
85-89	5.725703084636075E-4
90-94	0.001145140616927215
95-99	2.2672247113659626E-4
100-104	0.0020712442702139895
105-109	3.996463898001019E-4
110-114	6.801674134097888E-4
115-119	0.0016908116491542772
120-124	0.003443107358277801
125-129	0.026995345080247268
130-134	0.022633819576178844
135-139	0.012254541702620431
140-144	0.010360064104818024
145-149	0.009222608995386966
150-154	0.01087115077106394
155-159	0.0138723623537902
160-164	0.016892822725350292
165-169	0.013334466698956314
170-174	0.011743641832450817
175-179	0.013330892858933905
180-184	0.007393834395249099
185-189	0.008408783397003221
190-194	0.007083567610577353
195-199	0.005812421174804107
200-204	0.003664964976894761
205-209	2.2707693027706772E-4
210-214	8.057412572832841E-4
215-219	7.820139577103111E-4
220-224	0.0010879499521185038
225-229	5.401384575940254E-4
230-234	3.5383366512610975E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
152-153	1.0
154-155	6.0
156-157	3.0
158-159	9.0
160-161	4.0
162-163	6.0
164-165	8.0
166-167	13.0
168-169	15.0
170-171	13.0
172-173	29.0
174-175	26.0
176-177	28.0
178-179	41.0
180-181	57.0
182-183	85.0
184-185	103.0
186-187	130.0
188-189	171.0
190-191	243.0
192-193	310.0
194-195	376.0
196-197	498.0
198-199	661.0
200-201	1073.0
202-203	1486.0
204-205	1586.0
206-207	2063.0
208-209	3056.0
210-211	4171.0
212-213	5962.0
214-215	8771.0
216-217	12930.0
218-219	14577.0
220-221	16105.0
222-223	18405.0
224-225	21937.0
226-227	28643.0
228-229	37959.0
230-231	45581.0
232-233	52484.0
234-235	57692.0
236-237	63710.0
238-239	71012.0
240-241	80914.0
242-243	97758.0
244-245	127933.0
246-247	198374.0
248-249	427621.0
250-251	1940275.0
252-253	1859687.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.76333059294139
#Duplication Level	Percentage of total
1	26.653513482703367
2	10.183479304108001
3	5.338758393609535
4	3.618308244029098
5	2.870059808828293
6	2.5442729596782248
7	2.135190551371954
8	1.8915885954371447
9	1.7430960074259854
>10	28.009863168598304
>50	8.564462236678716
>100	6.337347401360664
>500	0.11005984617066972
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9213768740389512E-5	0.0	0.0	0.0	0.0
3	0.0	1.9213768740389512E-5	0.0	0.0	0.0	0.0
4	0.0	1.9213768740389512E-5	0.0	0.0	1.9213768740389512E-5	0.0
5	0.0	1.9213768740389512E-5	0.0	0.0	1.9213768740389512E-5	0.0
6	0.0	1.9213768740389512E-5	0.0	0.0	1.9213768740389512E-5	1.9213768740389512E-5
7	0.0	1.9213768740389512E-5	0.0	0.0	1.9213768740389512E-5	1.9213768740389512E-5
8	0.0	1.9213768740389512E-5	0.0	1.9213768740389512E-5	1.9213768740389512E-5	3.8427537480779024E-5
9	0.0	1.9213768740389512E-5	0.0	3.8427537480779024E-5	3.8427537480779024E-5	5.764130622116854E-5
10-14	0.0	2.3056522488467416E-5	0.0	9.606884370194757E-5	4.227029122885693E-5	7.301232121348016E-5
15-19	0.0	3.8427537480779024E-5	0.0	1.8445217990773933E-4	3.304768223346996E-4	1.1912536619041497E-4
20-24	0.0	3.8427537480779024E-5	0.0	9.683739445156315E-4	6.801674134097888E-4	1.8829493365581725E-4
25-29	0.0	3.8427537480779024E-5	0.0	0.03749374831999609	8.223493020886712E-4	2.1519420989236254E-4
30-34	0.0	3.8427537480779024E-5	0.0	0.7551971803410098	0.0010183297432406445	2.7667826986160895E-4
35-39	0.0	4.611304497693483E-5	7.685507496155805E-6	2.7902081254643725	0.0010721282957137346	3.15105807342388E-4
40-44	0.0	5.764130622116854E-5	1.9213768740389512E-5	6.825368553708536	0.0011412978631791372	3.3431957608277753E-4
45-49	0.0	5.764130622116854E-5	1.9213768740389512E-5	11.223780651004756	0.001218152938140695	3.5353334482316704E-4
50-54	0.0	5.764130622116854E-5	1.9213768740389512E-5	15.767149105185968	0.001348806565575344	4.034891435481798E-4
55-59	0.0	5.764130622116854E-5	1.9213768740389512E-5	20.17846132681449	0.0015102022229946156	4.457594347770367E-4
60-64	0.0	5.764130622116854E-5	1.9213768740389512E-5	24.57626626901851	0.001890634844054328	5.495137859751401E-4
65-69	0.0	5.764130622116854E-5	5.764130622116854E-5	28.81635306914017	0.0023940355850525334	5.956268309520749E-4
70-74	0.0	5.764130622116854E-5	6.148405996924644E-5	33.030977782927074	0.0026053870411968183	5.994695847001527E-4
75-79	0.0	5.764130622116854E-5	7.685507496155805E-5	37.04106424296502	0.002893593572302661	6.148405996924644E-4
80-84	0.0	5.764130622116854E-5	7.685507496155805E-5	40.951661808465246	0.0031818001034085034	6.148405996924644E-4
85-89	0.0	5.764130622116854E-5	7.685507496155805E-5	44.66240927978917	0.0034853776495066572	6.148405996924644E-4
90-94	0.0	5.764130622116854E-5	7.685507496155805E-5	48.19008796255467	0.0058909414958034255	6.378971221809318E-4
95-99	0.0	5.764130622116854E-5	7.685507496155805E-5	51.51504601409407	0.0764285292955214	7.339659658828794E-4
100-104	0.0	5.764130622116854E-5	7.685507496155805E-5	54.63980812361986	0.31366093193311073	8.185065483405933E-4
105-109	0.0	5.764130622116854E-5	7.685507496155805E-5	57.56466634041688	0.8025706485473142	8.761478545617618E-4
110-114	0.0	5.764130622116854E-5	7.685507496155805E-5	60.307374186801255	1.2965604856164767	9.030471307983071E-4
115-119	0.0	6.148405996924644E-5	7.685507496155805E-5	61.875974738505406	1.6615990351613887	9.222608995386966E-4
120-124	0.0	8.454058245771386E-5	7.685507496155805E-5	61.91566269921556	1.6969485268899576	9.337891607829303E-4
125-129	0.0	9.606884370194757E-5	9.606884370194757E-5	61.916216055755285	1.6970599667486517	9.799022057598653E-4
130-134	0.0	9.606884370194757E-5	9.606884370194757E-5	61.91714600216231	1.6971445073311096	0.0010029587282483327
135-139	0.0	1.1143985869425917E-4	9.606884370194757E-5	61.91963226383733	1.6973366450185132	0.0010183297432406443
140-144	0.0	1.3065362743464867E-4	9.606884370194757E-5	61.92438190746994	1.6976940211170846	0.0010337007582329556
145-149	0.0	1.537101499231161E-4	9.606884370194757E-5	61.93126427943277	1.6980706109843964	0.0010874993107060464
150-154	0.0	1.537101499231161E-4	1.0759710494618127E-4	61.942800226184474	1.6982320066418155	0.0011374551094310592
155-159	0.0	1.537101499231161E-4	1.1528261244233708E-4	61.95935865208495	1.6984318298367156	0.001175882646911838
160-164	0.0	1.537101499231161E-4	1.1528261244233708E-4	61.978875998371436	1.6985125276654252	0.0011950964156522276
165-169	0.0	1.537101499231161E-4	1.1528261244233708E-4	62.00262805928831	1.6987200363678217	0.001218152938140695
170-174	0.0	1.537101499231161E-4	1.1912536619041497E-4	62.02717941298478	1.6989160168089736	0.0012296811993849288
175-179	0.0	1.537101499231161E-4	1.3449638118272659E-4	62.058125108918055	1.6990735697126447	0.0012296811993849288
180-184	0.0	1.537101499231161E-4	1.3449638118272659E-4	62.10004186680209	1.6993118204450255	0.0012412094606291625
185-189	0.0	1.537101499231161E-4	1.3449638118272659E-4	62.14308070878055	1.6994347885649639	0.001260423229369552
190-194	0.0	1.613956574192719E-4	1.421818886788824E-4	62.18463624781227	1.6996691965435968	0.0012873225056060974
195-199	0.0	1.7292391866350563E-4	1.537101499231161E-4	62.22592663683537	1.7007028973018297	0.0013026935205984092
200-204	0.0	1.7292391866350563E-4	1.537101499231161E-4	62.266798165699925	1.706052010519154	0.0013219072893387986
205-209	0.0	1.7676667241158352E-4	1.537101499231161E-4	62.30570604739921	1.7277443554270537	0.0013334355505830323
210-214	0.0	1.9213768740389514E-4	1.537101499231161E-4	62.33765854481449	1.7648615138797386	0.001344963811827266
215-219	0.0	1.9213768740389514E-4	1.537101499231161E-4	62.37129801112515	1.8089110000939557	0.0013718630880638113
220-224	0.0	1.9213768740389514E-4	1.537101499231161E-4	62.40407670059626	1.8734961623379007	0.001383391349308045
225-229	0.0	1.9213768740389514E-4	1.537101499231161E-4	62.43547199871806	1.9399143181196792	0.0013910768568042007
230-234	0.0	1.9213768740389514E-4	1.537101499231161E-4	62.468369813555356	1.988659649414047	0.0014026051180484344
235-239	0.0	2.1135145614428465E-4	1.537101499231161E-4	62.50665132639371	2.0151746502757852	0.0014026051180484344
240-241	0.0	2.1135145614428465E-4	1.537101499231161E-4	62.57202041040226	2.016773235834985	0.0014026051180484344
>>END_MODULE
