##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665045
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172977
Total Bases	43 Mbp
Sequences flagged as poor quality	0
Sequence length	163-252
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.927262005931425	25.0	18.0	32.0	18.0	33.0
2	29.995831815790538	31.0	29.0	33.0	27.0	33.0
3	30.656439873509196	33.0	30.0	33.0	27.0	33.0
4	31.418460257722124	33.0	31.0	33.0	29.0	33.0
5	31.218508819091557	33.0	31.0	33.0	28.0	33.0
6	33.59818357353868	36.0	33.0	38.0	26.0	38.0
7	34.45634968810882	37.0	34.0	38.0	28.0	38.0
8	34.63066187990311	37.0	34.0	38.0	29.0	38.0
9	36.15958190973367	38.0	37.0	38.0	33.0	38.0
10-14	36.499612087156095	38.0	37.4	38.0	33.8	38.0
15-19	36.597399654289305	38.0	38.0	38.0	34.0	38.0
20-24	36.71966099539245	38.0	38.0	38.0	34.0	38.0
25-29	36.710759233886584	38.0	38.0	38.0	34.0	38.0
30-34	36.69352804130029	38.0	38.0	38.0	34.0	38.0
35-39	36.66659151216636	38.0	38.0	38.0	34.0	38.0
40-44	36.60447805199535	38.0	38.0	38.0	34.0	38.0
45-49	36.56680136665568	38.0	38.0	38.0	34.0	38.0
50-54	36.51030252576932	38.0	38.0	38.0	34.0	38.0
55-59	36.407459951323005	38.0	37.4	38.0	34.0	38.0
60-64	36.38655312556004	38.0	37.0	38.0	33.6	38.0
65-69	36.299068662307704	38.0	37.0	38.0	33.0	38.0
70-74	36.209241691091876	38.0	37.0	38.0	33.0	38.0
75-79	36.10841903836926	38.0	37.0	38.0	33.0	38.0
80-84	35.97147019545952	38.0	37.0	38.0	31.8	38.0
85-89	35.87589101441232	38.0	37.0	38.0	31.0	38.0
90-94	35.77533660544466	38.0	36.6	38.0	31.0	38.0
95-99	35.61191834752597	38.0	36.0	38.0	29.8	38.0
100-104	35.55470611699822	38.0	36.0	38.0	29.8	38.0
105-109	35.295916798187044	38.0	35.2	38.0	28.4	38.0
110-114	35.17135226070518	38.0	35.0	38.0	28.0	38.0
115-119	34.933596952195956	38.0	34.8	38.0	27.6	38.0
120-124	35.017404626048545	38.0	34.4	38.0	28.8	38.0
125-129	33.78652306375992	34.2	33.2	35.6	31.8	35.6
130-134	35.537883071159754	36.2	36.0	37.2	33.8	37.6
135-139	36.80710614706002	38.0	38.0	38.0	35.0	38.0
140-144	36.76058204269931	38.0	38.0	38.0	35.0	38.0
145-149	36.73358423374206	38.0	38.0	38.0	35.0	38.0
150-154	36.71186227070651	38.0	38.0	38.0	34.4	38.0
155-159	36.708609815177745	38.0	38.0	38.0	34.2	38.0
160-164	36.69429540443324	38.0	38.0	38.0	34.0	38.0
165-169	36.643599688225784	38.0	38.0	38.0	34.0	38.0
170-174	36.60666201529547	38.0	38.0	38.0	34.0	38.0
175-179	36.561768332959105	38.0	38.0	38.0	34.0	38.0
180-184	36.50146234807803	38.0	38.0	38.0	34.0	38.0
185-189	36.40321751819486	38.0	38.0	38.0	34.0	38.0
190-194	36.31027814167258	38.0	38.0	38.0	33.4	38.0
195-199	36.16792463905231	38.0	37.4	38.0	33.0	38.0
200-204	35.960002064962794	38.0	37.0	38.0	31.6	38.0
205-209	35.81233543641049	38.0	37.0	38.0	31.0	38.0
210-214	35.66866382608662	38.0	37.0	38.0	29.8	38.0
215-219	35.443494370473545	38.0	36.6	38.0	28.6	38.0
220-224	35.33447987640104	38.0	36.0	38.0	28.0	38.0
225-229	35.09304649774739	38.0	35.8	38.0	26.6	38.0
230-234	34.885227596192706	38.0	35.2	38.0	25.0	38.0
235-239	34.75014715612993	38.0	35.0	38.0	24.2	38.0
240-244	34.71410804062326	38.0	34.8	38.0	24.8	38.0
245-249	35.075522766433224	38.0	35.2	38.0	28.0	38.0
250-252	35.977009834104024	38.0	35.0	38.0	31.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	11.0
21	28.0
22	82.0
23	201.0
24	375.0
25	583.0
26	835.0
27	1351.0
28	1813.0
29	2303.0
30	3083.0
31	4136.0
32	5494.0
33	7726.0
34	12029.0
35	18896.0
36	35493.0
37	78536.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.70106588586573	20.263404585276657	16.34763387225572	23.687895656601896
2	14.80138978014418	22.49547627719292	39.2063684767339	23.496765465928995
3	16.717251426490225	28.05806552316204	32.15803257080421	23.066650479543522
4	12.811529856570528	19.56040398434474	38.56524277794158	29.06282338114316
5	14.873653722749266	34.82081432791643	35.67699751990149	14.628534429432815
6	28.875515241910776	38.331685715441935	19.573700549784075	13.219098492863212
7	23.662683478150274	31.396659671517025	26.89837377223562	18.04228307809709
8	23.01404232932702	35.71515288159697	22.33649560346173	18.934309185614275
9	23.268411407296924	16.547286633483065	21.046728755846154	39.13757320337386
10-14	23.66054136802319	23.399812460182474	29.12925794587245	23.810388225921887
15-19	23.239158963330386	28.1100955618377	26.090751949681174	22.55999352515074
20-24	22.991611601542402	27.075969637581878	26.836400215057495	23.096018545818232
25-29	23.2367332969503	26.92895596111332	26.629374057664023	23.204936684272354
30-34	23.145265071148515	26.787615868541454	26.913066653100255	23.154052407209775
35-39	22.98077612382091	26.624710069352105	27.037261435928507	23.35725237089848
40-44	22.829646314445746	26.341872910008373	27.411555485664334	23.416925289881547
45-49	22.465433791749433	26.263265122065715	27.688810469184677	23.58249061700017
50-54	22.188524703890927	26.296011950735593	27.57699298403023	23.938470361343256
55-59	21.967917084834497	26.08858529005046	27.53305645612591	24.410441168989134
60-64	22.056111506153997	26.1218543505784	27.037929898194555	24.784104245073042
65-69	21.904299415529234	26.151338039161274	26.661694907415434	25.282667637894058
70-74	21.759424663394554	25.847598235603574	26.57301259705048	25.819964503951393
75-79	21.392001701962343	25.746689729489734	26.828337673028983	26.032970895518936
80-84	21.02718858576574	25.613578683871268	26.99873393572556	26.360498794637437
85-89	21.04501875396007	25.298999634633084	27.062704362665972	26.593277248740872
90-94	21.00605399105545	24.92981717779494	27.212088557540664	26.852040273608946
95-99	20.934118027934513	24.644806215891222	27.52439644806216	26.89667930811211
100-104	20.859377348586282	24.781993381756124	27.468718276019153	26.889910993638438
105-109	20.49454030829568	25.619505043450914	27.28527756323391	26.60067708501949
110-114	20.314492678217334	26.346277250732758	27.001162004197088	26.33806806685282
115-119	20.124664116806414	26.4928013467702	27.118203134755642	26.26433140166774
120-124	20.19505483387965	26.64724211889442	26.99040912953745	26.16729391768848
125-129	22.11426952716257	25.013730149094965	28.54171363822936	24.3302866855131
130-134	21.65081244457189	31.25792759282149	27.42712974022355	19.66413022238307
135-139	23.96127456761688	23.270294082959282	28.054200805218894	24.714230544204945
140-144	23.02597651868862	27.16862190093634	26.170423352735362	23.63497822763968
145-149	22.931741395697326	27.100980739034615	27.022817618614848	22.944460246653207
150-154	23.11208290069169	26.90977080206558	26.817268356532175	23.16087794071056
155-159	22.934586420088547	26.75801958950154	26.93180675052697	23.37558723988294
160-164	22.867370913732817	26.74412151036945	27.105225426895824	23.283282149001906
165-169	22.82938865936587	26.64340216711357	26.946349427695626	23.580859745824938
170-174	22.833537614763767	26.748687588168636	26.923984181679423	23.493790615388175
175-179	22.903638365187145	26.395261706969602	26.928458435863757	23.7726414919795
180-184	22.986810988337467	26.401614367407355	26.840939709853306	23.77063493440187
185-189	22.924442213542783	26.256183330769595	27.142349902790002	23.67702455289762
190-194	22.930906819075826	26.384640303584238	26.89254228660018	23.791910590739757
195-199	22.894922066736434	26.267862600368595	27.172684923641143	23.66453040925382
200-204	23.081076759247136	26.06451702613103	26.809594934860726	24.04481127976111
205-209	23.220787299951372	25.745732083105562	27.03553724859361	23.99794336834946
210-214	23.44890489719087	26.0792224804957	26.529455973679372	23.94241664863406
215-219	23.586534991995123	26.56824930840436	26.046385077181956	23.79883062241856
220-224	23.76689250687327	26.41813674195848	26.48202497513174	23.33294577603651
225-229	23.506907696300765	26.756256747996193	26.30708167756815	23.429753878134893
230-234	23.684922919957792	26.891207232397562	26.109999530770057	23.313870316874592
235-239	23.86950435567714	26.91921639633833	26.140828230593804	23.070451017390727
240-244	23.60807832542834	26.76351550360041	26.377575892993164	23.250830277978082
245-249	23.27420953164852	27.248347042546396	26.09642116435352	23.38102226145157
250-252	23.095999723750836	27.054210759277108	26.526825991279317	23.322963525692742
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.3333333333333333
16	0.6666666666666666
17	0.3333333333333333
18	0.6666666666666666
19	1.6666666666666665
20	3.666666666666667
21	6.999999999999997
22	11.000000000000002
23	15.000000000000009
24	29.99999999999996
25	40.00000000000001
26	63.66666666666684
27	92.8333333333331
28	143.49999999999972
29	238.66666666666907
30	343.6666666666646
31	483.6666666666566
32	648.6666666666705
33	872.666666666696
34	1116.5000000000255
35	1414.4999999999577
36	1794.4999999998713
37	2211.8333333332216
38	2595.0000000000623
39	3078.6666666669485
40	3550.3333333338296
41	4067.0000000007317
42	4429.166666667108
43	4733.500000000164
44	4988.999999999931
45	5044.166666666549
46	4960.166666666624
47	4757.333333333472
48	4378.000000000481
49	3949.5000000006717
50	3386.8333333337523
51	2935.166666666884
52	2621.6666666667415
53	2270.83333333325
54	1973.1666666664987
55	1698.3333333332275
56	1474.4999999999443
57	1291.833333333319
58	1201.6666666666727
59	1112.5000000000264
60	986.5000000000422
61	905.500000000033
62	841.3333333333592
63	729.000000000013
64	633.000000000002
65	557.6666666666601
66	448.9999999999919
67	336.9999999999983
68	274.1666666666685
69	201.50000000000134
70	155.00000000000003
71	115.99999999999943
72	79.66666666666661
73	56.00000000000012
74	42.66666666666669
75	29.333333333333297
76	17.33333333333334
77	13.666666666666673
78	8.999999999999998
79	4.666666666666666
80	2.333333333333333
81	2.333333333333333
82	0.3333333333333333
83	0.6666666666666666
84	0.3333333333333333
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09423218115703244
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	2.312446163362759E-4
15-19	0.0
20-24	0.0
25-29	0.0015030900061857935
30-34	4.624892326725518E-4
35-39	0.002659313087867173
40-44	0.00566549310023876
45-49	0.002659313087867173
50-54	1.1562230816813795E-4
55-59	1.1562230816813795E-4
60-64	0.0
65-69	0.0
70-74	0.0
75-79	1.1562230816813795E-4
80-84	0.0
85-89	1.1562230816813795E-4
90-94	1.1562230816813795E-4
95-99	5.781115408406898E-4
100-104	3.4686692450441387E-4
105-109	1.1562230816813795E-4
110-114	0.0
115-119	1.1562230816813795E-4
120-124	0.0
125-129	0.0
130-134	0.003006180012371587
135-139	0.003121802320539725
140-144	0.003121802320539725
145-149	0.003121802320539725
150-154	0.004971759251229932
155-159	0.0039311584777166906
160-164	0.004162407906725064
165-169	0.00416246084685266
170-174	0.005434361583919839
175-179	0.0077469991940808305
180-184	0.007631813561732699
185-189	0.005204309630979752
190-194	0.004049168474331164
195-199	0.0041673805235156024
200-204	0.0033602695631417827
205-209	0.0040620306903827366
210-214	0.006165608237252604
215-219	0.014010949557078855
220-224	0.015030266023966229
225-229	0.018840469803111166
230-234	0.028506614256193875
235-239	0.0805047092182194
240-244	0.03540757417506063
245-249	0.0012183813127381767
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
162-163	1.0
164-165	1.0
166-167	1.0
168-169	0.0
170-171	0.0
172-173	2.0
174-175	1.0
176-177	1.0
178-179	2.0
180-181	6.0
182-183	10.0
184-185	11.0
186-187	12.0
188-189	23.0
190-191	27.0
192-193	42.0
194-195	47.0
196-197	42.0
198-199	59.0
200-201	74.0
202-203	112.0
204-205	116.0
206-207	127.0
208-209	150.0
210-211	181.0
212-213	216.0
214-215	259.0
216-217	301.0
218-219	369.0
220-221	423.0
222-223	544.0
224-225	628.0
226-227	762.0
228-229	945.0
230-231	1140.0
232-233	1344.0
234-235	1399.0
236-237	1664.0
238-239	1911.0
240-241	2273.0
242-243	2875.0
244-245	3809.0
246-247	5722.0
248-249	11629.0
250-251	64877.0
252-253	68839.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.01953514636625
#Duplication Level	Percentage of total
1	98.07808407807882
2	1.8149285599644072
3	0.09288937362803217
4	0.004081321936080989
5	0.010016666392669477
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0	0.0
15-19	2.3124461633627593E-4	0.0	0.0	0.0	0.0	0.0
20-24	0.0011562230816813796	0.0	0.0	8.093561571769659E-4	0.0	0.0
25-29	0.0011562230816813796	0.0	2.3124461633627593E-4	0.030408667048220282	0.0	5.781115408406898E-4
30-34	0.0011562230816813796	0.0	5.781115408406898E-4	0.36444151534597086	4.6248923267255186E-4	5.781115408406898E-4
35-39	0.0011562230816813796	0.0	5.781115408406898E-4	1.2139186134572806	5.781115408406898E-4	5.781115408406898E-4
40-44	0.0011562230816813796	1.1562230816813797E-4	6.937338490088277E-4	2.8971481757690327	0.0010406007735132418	5.781115408406898E-4
45-49	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	4.754736178798337	0.0013874676980176555	5.781115408406898E-4
50-54	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	6.762170693213548	0.0019655792388583457	5.781115408406898E-4
55-59	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	8.66774195413263	0.003121802320539725	5.781115408406898E-4
60-64	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	10.69772281864063	0.003931158477716691	9.249784653451037E-4
65-69	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	12.762621620215404	0.004971759251229933	0.0011562230816813796
70-74	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	14.897471918231904	0.0057811154084068984	0.0011562230816813796
75-79	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	17.07163380102557	0.006243604641079449	0.0011562230816813796
80-84	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	19.273891904704094	0.006706093873752002	0.0011562230816813796
85-89	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	21.391167611879034	0.007515450030928968	0.0011562230816813796
90-94	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	23.578163570879365	0.009018540037114761	0.0011562230816813796
95-99	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	25.726310434335204	0.02150574931927366	0.0011562230816813796
100-104	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	27.83271764454234	0.0724951872214225	0.0011562230816813796
105-109	0.0011562230816813796	5.781115408406898E-4	0.0011562230816813796	29.952305797880644	0.16765234684380004	0.0011562230816813796
110-114	0.0011562230816813796	5.781115408406898E-4	0.0012718453898495174	32.0709689727536	0.2542534556617354	0.0011562230816813796
115-119	0.0011562230816813796	5.781115408406898E-4	0.0017343346225220696	33.32893968562294	0.3299860675118657	0.0011562230816813796
120-124	0.0011562230816813796	5.781115408406898E-4	0.0017343346225220696	33.36027333113652	0.3381952513918035	0.0011562230816813796
125-129	0.0011562230816813796	5.781115408406898E-4	0.0017343346225220696	33.36108268729369	0.3381952513918035	0.0011562230816813796
130-134	0.0011562230816813796	5.781115408406898E-4	0.0017343346225220696	33.36119830960185	0.3381952513918035	0.0011562230816813796
135-139	0.0011562230816813796	5.781115408406898E-4	0.0017343346225220696	33.36316388884071	0.3381952513918035	0.0011562230816813796
140-144	0.0011562230816813796	5.781115408406898E-4	0.0017343346225220696	33.36339513345705	0.3381952513918035	0.0011562230816813796
145-149	0.0011562230816813796	5.781115408406898E-4	0.0017343346225220696	33.364898223463236	0.3381952513918035	0.0011562230816813796
150-154	0.0011562230816813796	6.937338490088277E-4	0.0017343346225220696	33.371835561953326	0.3381952513918035	0.0011562230816813796
155-159	0.0017343346225220696	0.0011562230816813796	0.0017343346225220696	33.37657607658822	0.3381952513918035	0.0011562230816813796
160-164	0.0017343346225220696	0.0011562230816813796	0.0017343346225220696	33.38559461662534	0.3381952513918035	0.0011562230816813796
165-169	0.0017343346225220696	0.0011562230816813796	0.0017343346225220696	33.39472877897062	0.3381952513918035	0.0011562230816813796
170-174	0.0017343346225220696	0.0011562230816813796	0.0017343346225220696	33.40559727593842	0.3381952513918035	0.0011562230816813796
175-179	0.0017343346225220696	0.0011562230816813796	0.0017343346225220696	33.41646577290622	0.3381952513918035	0.0011562230816813796
180-184	0.0017343346225220696	0.0011562230816813796	0.0017343346225220696	33.43126542835175	0.3381952513918035	0.0011562230816813796
185-189	0.0017343346225220696	0.0011562230816813796	0.0017343346225220696	33.449996242274985	0.3387733629326442	0.0011562230816813796
190-194	0.0027749353960353114	0.0015030900061857933	0.0017343346225220696	33.46965203466357	0.33923585216531676	0.0016187123143539314
195-199	0.0028905577042034492	0.0017343346225220696	0.0017343346225220696	33.48711100319695	0.3401608306306619	0.0017343346225220696
200-204	0.0028905577042034492	0.0017343346225220696	0.0017343346225220696	33.5058418171202	0.34802314758609526	0.0017343346225220696
205-209	0.0028905577042034492	0.0017343346225220696	0.0017343346225220696	33.52376327488626	0.36871954074819197	0.0017343346225220696
210-214	0.0028905577042034492	0.0017343346225220696	0.0017343346225220696	33.54191597726866	0.4042155893558103	0.0017343346225220696
215-219	0.0028905577042034492	0.0017343346225220696	0.0017343346225220696	33.56064679119189	0.44641773183718064	0.0017343346225220696
220-224	0.0028905577042034492	0.0017343346225220696	0.0017343346225220696	33.58377125282552	0.519606652907612	0.0017343346225220696
225-229	0.0028905577042034492	0.0017343346225220696	0.0017343346225220696	33.60620198061014	0.6191574602403788	0.0017343346225220696
230-234	0.0028905577042034492	0.0017343346225220696	0.0017343346225220696	33.63603253611752	0.6991680974927302	0.0017343346225220696
235-239	0.0028905577042034492	0.0017343346225220696	0.0017343346225220696	33.669331760869945	0.7696977054752944	0.0017343346225220696
240-241	0.0028905577042034492	0.0017343346225220694	0.0017343346225220694	33.7420581927077	0.7712007954814802	0.0017343346225220694
>>END_MODULE
