##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665052
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3606169
Total Bases	893.9 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.49006577340108	32.0	18.0	33.0	18.0	33.0
2	27.181018415942237	29.0	25.0	31.0	18.0	33.0
3	26.794588107212945	29.0	25.0	31.0	18.0	33.0
4	30.117362220128896	31.0	29.0	33.0	27.0	33.0
5	31.44694327969654	33.0	31.0	33.0	29.0	33.0
6	35.57575809675032	37.0	36.0	38.0	29.0	38.0
7	35.910478682502124	38.0	36.0	38.0	31.0	38.0
8	36.46381381460492	38.0	37.0	38.0	34.0	38.0
9	36.69241014494884	38.0	38.0	38.0	34.0	38.0
10-14	36.78561121234196	38.0	38.0	38.0	34.4	38.0
15-19	36.792350829925056	38.0	38.0	38.0	34.8	38.0
20-24	36.80307351097522	38.0	38.0	38.0	35.0	38.0
25-29	36.74167783040672	38.0	38.0	38.0	34.2	38.0
30-34	36.717041547415	38.0	38.0	38.0	34.0	38.0
35-39	36.68360295926231	38.0	38.0	38.0	34.0	38.0
40-44	36.65372770937801	38.0	38.0	38.0	34.0	38.0
45-49	36.63089960564799	38.0	38.0	38.0	34.0	38.0
50-54	36.580150569759766	38.0	38.0	38.0	34.0	38.0
55-59	36.4994987201099	38.0	38.0	38.0	34.0	38.0
60-64	36.44937588892812	38.0	38.0	38.0	34.0	38.0
65-69	36.37166133922176	38.0	37.6	38.0	33.4	38.0
70-74	36.27823759784969	38.0	37.0	38.0	33.0	38.0
75-79	36.1546640278447	38.0	37.0	38.0	33.0	38.0
80-84	36.030054573776674	38.0	37.0	38.0	32.6	38.0
85-89	35.88963538174933	38.0	37.0	38.0	31.0	38.0
90-94	35.7777153946695	38.0	36.8	38.0	31.0	38.0
95-99	35.651614845133956	38.0	36.0	38.0	31.0	38.0
100-104	35.51780874051941	38.0	36.0	38.0	29.0	38.0
105-109	35.33269940266826	38.0	35.4	38.0	28.8	38.0
110-114	35.17385630027417	38.0	35.0	38.0	28.0	38.0
115-119	35.01720229828072	38.0	35.0	38.0	28.0	38.0
120-124	35.074915139735126	38.0	34.6	38.0	28.8	38.0
125-129	33.33062344902667	34.2	32.8	35.6	29.4	35.6
130-134	34.60414316635754	36.2	35.4	37.0	30.2	37.6
135-139	35.797254694096516	38.0	37.0	38.0	29.4	38.0
140-144	35.71133242957706	38.0	37.0	38.0	29.0	38.0
145-149	35.67795947914893	38.0	37.0	38.0	29.0	38.0
150-154	35.649254063316874	38.0	37.0	38.0	29.0	38.0
155-159	35.63171006768052	38.0	37.0	38.0	29.0	38.0
160-164	35.60574816246283	38.0	37.0	38.0	29.0	38.0
165-169	35.535815898336296	38.0	37.0	38.0	29.0	38.0
170-174	35.48163170325887	38.0	36.4	38.0	29.0	38.0
175-179	35.40551673613878	38.0	36.0	38.0	28.2	38.0
180-184	35.301663523549855	38.0	36.0	38.0	28.0	38.0
185-189	35.18075396131381	38.0	36.0	38.0	27.2	38.0
190-194	35.0226039156731	38.0	36.0	38.0	27.0	38.0
195-199	34.862416662525334	38.0	35.4	38.0	25.8	38.0
200-204	34.62949254564853	38.0	35.0	38.0	24.8	38.0
205-209	34.434736634818336	38.0	35.0	38.0	23.2	38.0
210-214	34.245816591955844	38.0	34.0	38.0	23.0	38.0
215-219	33.99274116675487	38.0	34.0	38.0	21.8	38.0
220-224	33.79258049624557	38.0	34.0	38.0	21.0	38.0
225-229	33.554508097464165	38.0	33.8	38.0	17.2	38.0
230-234	33.34748031580332	38.0	33.0	38.0	15.0	38.0
235-239	33.1812581570778	38.0	32.6	38.0	15.0	38.0
240-244	33.17152505033512	38.0	32.0	38.0	18.0	38.0
245-249	33.63193452509173	38.0	32.8	38.0	22.0	38.0
250-252	35.36171209080799	37.666666666666664	33.333333333333336	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	8.0
17	24.0
18	36.0
19	85.0
20	407.0
21	1665.0
22	4581.0
23	9733.0
24	17019.0
25	26138.0
26	37331.0
27	49505.0
28	63343.0
29	78478.0
30	96680.0
31	118647.0
32	148779.0
33	193797.0
34	273892.0
35	406117.0
36	709882.0
37	1370022.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.9915927691379	16.831743315935235	11.877862697060076	22.29880121786678
2	17.192087204141583	19.173475157362724	35.505450536152175	28.128987102343515
3	21.138623278710163	26.171758229386256	27.88923514806046	24.800383343843123
4	14.592752401446413	18.54379728020942	36.527220090068106	30.33623022827606
5	17.046816796689445	35.04766700784185	31.830646590295558	16.074869605173145
6	30.660713523130685	35.54998361138518	19.218326852759247	14.570976012724888
7	26.023045508959786	29.31587510180471	24.715536071659425	19.945543317576075
8	24.893314761454608	32.72874898541915	21.034066900358802	21.343869352767438
9	23.781553221715342	16.23867322912487	20.027763535208692	39.952010013951096
10-14	25.214395400201788	22.176460774909287	27.17338712897733	25.4357566959116
15-19	24.76343732087986	26.521424813978488	24.592813037880365	24.122324827261284
20-24	24.522022702788142	25.532275977388725	25.401344641745453	24.54435667807768
25-29	24.768937948151898	25.427307977765157	25.157214705488574	24.64653936859437
30-34	24.583841989543075	25.296917403327313	25.45213346096257	24.667107146167037
35-39	24.431476143755866	25.069480302313497	25.71831068718251	24.780732866748124
40-44	24.06817419533369	24.791971882085313	26.135761888959262	25.004092033621735
45-49	23.5225610203189	24.806584249843066	26.588355265339953	25.08249946449808
50-54	23.12046932403837	24.98388026908332	26.420535688635017	25.475114718243297
55-59	22.87687857366997	24.80795952089626	26.257798169513404	26.05736373592037
60-64	23.03289976113135	24.86839485481256	25.691790117260272	26.406915266795817
65-69	23.045521330005407	24.942099216209733	25.19570354000068	26.81667591378418
70-74	23.209216490185504	24.884273926866502	24.91910890993013	26.987400673017863
75-79	22.987469770894382	24.721629342414765	25.0307587597021	27.260142126988757
80-84	22.741540319291335	24.498090798840646	25.31102790928968	27.44934097257834
85-89	22.648519196591916	24.05299081672434	25.731613495648688	27.566876491035057
90-94	22.655267892998108	23.628514091866144	26.00259916888718	27.713618846248565
95-99	22.41959314132004	23.535823431029506	26.356978147696257	27.687605279954198
100-104	22.271698738236054	23.912681357523528	26.418891607582644	27.39672829665778
105-109	21.948480830418976	25.02821711598523	26.113246707827457	26.910055345768335
110-114	21.676394167183986	26.02604286077959	25.748988317011833	26.54857465502459
115-119	21.614253521989724	26.289113393850368	25.665726596230943	26.43090648792897
120-124	21.728683085907754	26.357190525385406	25.559417419446994	26.35470896925985
125-129	23.894596783479166	24.16890554804238	26.4802981685239	25.45619949995456
130-134	23.326132248856958	29.292327020634534	25.785969522805917	21.595571207702587
135-139	25.286725536326998	21.925381279394536	26.403305602437264	26.384587581841203
140-144	24.344704802342267	25.436204720201093	24.959223272630197	25.259867204826435
145-149	24.273260129702663	25.4008453909082	25.663608799833366	24.66228567955577
150-154	24.339975305687382	25.260903590791933	25.588534414160748	24.810586689359944
155-159	24.174503279179945	25.12675705768646	25.72690712444996	24.97183253868364
160-164	24.03527057001929	25.117852586901957	25.88106928775596	24.965807555322794
165-169	23.922463942952493	24.983146721210698	25.844670845897273	25.24971848993954
170-174	23.892665778619563	25.056878041078985	25.867318052245402	25.183138128056047
175-179	23.90120971111559	24.697800636666603	25.81748816417397	25.583501488043837
180-184	23.945498607986345	24.581012776640048	25.902557985618706	25.5709306297549
185-189	23.58021540560179	24.183290909009855	26.561523441853414	25.674970243534933
190-194	23.576777832057488	24.438567321865623	26.29399136989774	25.69066347617915
195-199	23.357238897320446	24.276006531178258	26.61918050623101	25.74757406527029
200-204	23.706646590510957	24.10561664174267	26.315108408784532	25.87262835896184
205-209	23.772074083968533	23.812415023334843	26.439334244224106	25.97617664847252
210-214	23.823301250319773	24.005826778637186	26.23184548799439	25.93902648304865
215-219	23.562424120085872	24.269784311238485	26.082476468074283	26.085315100601363
220-224	23.750261695907426	24.23308072164787	26.542843390962005	25.473814191482695
225-229	23.439454945253438	24.61667779456402	26.312188838402818	25.631678421779725
230-234	23.56971927000211	24.871919790663174	26.214848136442644	25.34351280289207
235-239	23.576626013586637	24.765253275584357	26.495008149350145	25.16311256147887
240-244	23.461344936636145	24.737254474297174	26.25171701398614	25.54968357508054
245-249	23.043207649026595	25.246234779110647	26.38411650613643	25.326441065726325
250-252	22.33530648891294	25.424385241694992	26.560335774278887	25.67997249511318
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	0.0
16	1.6666666666666665
17	4.666666666666666
18	6.333333333333331
19	11.0
20	20.666666666666664
21	48.500000000000064
22	85.16666666666656
23	132.166666666666
24	224.8333333333353
25	382.83333333332934
26	631.0000000000007
27	1040.5000000000462
28	1636.9999999999109
29	2442.8333333333176
30	3580.0000000004975
31	5170.333333333134
32	7330.49999999785
33	10130.833333333749
34	13836.500000007123
35	18460.833333338098
36	24196.666666650694
37	30884.333333293227
38	38382.66666665814
39	46636.33333338481
40	55368.00000011465
41	64360.6666668463
42	72812.16666675718
43	79956.83333332065
44	84947.16666658201
45	88006.49999987181
46	88836.49999986049
47	87723.6666665442
48	84724.33333325513
49	80540.9999999822
50	76372.66666670925
51	72076.83333343809
52	67771.66666683403
53	63356.666666835095
54	59287.33333347243
55	55597.000000112675
56	51986.16666675366
57	48690.00000006349
58	45936.00000004389
59	43009.333333356364
60	40136.00000000232
61	36932.49999997929
62	33243.83333328621
63	29279.33333329976
64	24903.166666648827
65	20552.83333333116
66	16283.833333344599
67	12599.00000000467
68	9324.999999998792
69	6705.49999999849
70	4718.000000000264
71	3212.3333333336427
72	2143.833333333164
73	1362.8333333333119
74	844.1666666666893
75	505.8333333333235
76	282.33333333333564
77	157.4999999999998
78	75.50000000000011
79	36.16666666666662
80	16.000000000000007
81	4.5
82	0.6666666666666666
83	0.3333333333333333
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03263851472296501
2	2.7730258898016146E-4
3	1.9411181228611303E-4
4	2.495723300821453E-4
5	1.6638155338809688E-4
6	1.9411181228611303E-4
7	0.0
8	0.0
9	0.0
10-14	7.819933009240555E-4
15-19	0.0
20-24	1.1092103559206459E-4
25-29	0.0022572430742985143
30-34	0.002739749579123996
35-39	0.0030780587376797925
40-44	0.010604051002601375
45-49	0.00571797938477093
50-54	8.152696116016748E-4
55-59	6.100656957563553E-5
60-64	8.319077669404844E-5
65-69	5.54605177960323E-6
70-74	6.655262135523875E-4
75-79	6.045229966925936E-4
80-84	0.0022961134453082415
85-89	0.0017526192367598352
90-94	0.004825368794062689
95-99	0.0022685514539085255
100-104	0.0015253937568240569
105-109	9.9303290334084E-4
110-114	4.1614604484534044E-4
115-119	0.001060009937454419
120-124	9.659655366812922E-4
125-129	0.0010462256436026781
130-134	0.0026070963714844935
135-139	0.002167009590827923
140-144	0.0011252851859826234
145-149	0.001426103998076988
150-154	0.0017269230987774385
155-159	0.0017603482993949137
160-164	0.0019386159541073895
165-169	0.001966478943132883
170-174	0.002612708862119053
175-179	0.003403833161813939
180-184	0.004089320338802976
185-189	0.0029755579644820907
190-194	0.0021738662702129154
195-199	0.0014946796098484663
200-204	0.0013676935924225085
205-209	0.0016438033561656127
210-214	0.003688833961266683
215-219	0.01106043185692712
220-224	0.012360272225357018
225-229	0.013803143678864963
230-234	0.026057151514003823
235-239	0.06990516796359673
240-244	0.02972644592028122
245-249	1.4791983363590783E-4
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	56.0
80-84	55.0
85-89	77.0
90-94	104.0
95-99	147.0
100-104	371.0
105-109	608.0
110-114	656.0
115-119	920.0
120-124	2091.0
125-129	10883.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	5.0
160-164	12.0
165-169	26.0
170-174	41.0
175-179	150.0
180-184	406.0
185-189	896.0
190-194	1649.0
195-199	2642.0
200-204	4671.0
205-209	7344.0
210-214	13492.0
215-219	20139.0
220-224	30835.0
225-229	47740.0
230-234	74558.0
235-239	103653.0
240-244	172560.0
245-249	486846.0
250-253	2622535.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.4124101916728
#Duplication Level	Percentage of total
1	84.67783274399144
2	11.068078459629454
3	2.5460289595426024
4	0.8730032949104374
5	0.35616346311390107
6	0.17627223354161173
7	0.09314015466009182
8	0.055991032589313025
9	0.037836267666717695
>10	0.11565339035440336
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1092103559206459E-4	0.0	0.0
3	0.0	0.0	0.0	1.1092103559206459E-4	0.0	0.0
4	0.0	0.0	0.0	1.1092103559206459E-4	0.0	0.0
5	0.0	2.7730258898016148E-5	0.0	1.3865129449008076E-4	0.0	0.0
6	0.0	2.7730258898016148E-5	0.0	1.663815533880969E-4	0.0	0.0
7	0.0	2.7730258898016148E-5	0.0	1.9411181228611306E-4	0.0	2.7730258898016148E-5
8	0.0	2.7730258898016148E-5	0.0	1.9411181228611306E-4	0.0	2.7730258898016148E-5
9	0.0	2.7730258898016148E-5	0.0	2.2184207118412918E-4	0.0	2.7730258898016148E-5
10-14	0.0	3.327631067761938E-5	0.0	4.2704598702944876E-4	0.0	2.7730258898016144E-5
15-19	2.218420711841292E-5	5.546051779603229E-5	5.54605177960323E-6	0.0011757629772758847	0.0	3.8822362457222604E-5
20-24	1.1092103559206458E-4	5.546051779603229E-5	2.7730258898016144E-5	0.007786656698562935	6.1006569575635536E-5	5.546051779603229E-5
25-29	1.4974339804928722E-4	6.655262135523875E-5	2.7730258898016144E-5	0.09492067620790928	9.428288025325492E-5	5.546051779603229E-5
30-34	1.885657605065098E-4	1.1092103559206458E-4	4.991446601642906E-5	0.7218740996331564	3.826775727926229E-4	5.546051779603229E-5
35-39	1.9965786406571628E-4	1.275591909308743E-4	9.428288025325492E-5	1.984094478101276	4.60322297707068E-4	5.546051779603229E-5
40-44	2.3293417474333565E-4	1.8856576050650984E-4	1.1092103559206458E-4	4.214799694634388	5.046907119438939E-4	5.546051779603229E-5
45-49	2.8284864075976473E-4	2.4957233008214536E-4	1.1092103559206458E-4	6.642289920411383	6.821643688911973E-4	8.319077669404845E-5
50-54	3.327631067761938E-4	2.6066443364135185E-4	1.1646708737166782E-4	9.10907946909865	0.0013865129449008077	1.1092103559206458E-4
55-59	3.6049336567421E-4	3.0503284787817766E-4	1.3865129449008076E-4	11.515982750669755	0.0020575852102327984	1.3310524271047754E-4
60-64	4.1040783169063905E-4	3.0503284787817766E-4	1.663815533880969E-4	13.901411719750238	0.0026121903881931212	1.4419734626968398E-4
65-69	4.3259203880905193E-4	3.0503284787817766E-4	1.7192760516770013E-4	16.229228302944204	0.003438552103354002	2.4402627830254214E-4
70-74	4.60322297707068E-4	3.0503284787817766E-4	1.9411181228611306E-4	18.54236448707756	0.004070802006228771	2.773025889801615E-4
75-79	4.714144012662745E-4	3.327631067761938E-4	1.9411181228611306E-4	20.798232140534733	0.004564400614613458	2.773025889801615E-4
80-84	4.714144012662745E-4	4.1595388347024227E-4	1.9965786406571628E-4	23.041787559041186	0.005152282103251401	3.216710032169874E-4
85-89	4.714144012662745E-4	4.3259203880905193E-4	2.2184207118412916E-4	25.230570170172278	0.006278130614510856	3.6049336567420997E-4
90-94	4.714144012662745E-4	4.60322297707068E-4	2.2184207118412916E-4	27.38406879988154	0.012162491552669883	3.771315210130196E-4
95-99	4.714144012662745E-4	4.714144012662745E-4	2.3293417474333565E-4	29.48812437797563	0.07218740996331564	3.882236245722261E-4
100-104	4.714144012662745E-4	4.714144012662745E-4	2.4957233008214536E-4	31.578603221313255	0.18680766209237562	3.882236245722261E-4
105-109	4.714144012662745E-4	4.880525566050843E-4	2.4957233008214536E-4	33.62441416361796	0.36710425939549696	3.882236245722261E-4
110-114	4.714144012662745E-4	5.213288672827036E-4	2.4957233008214536E-4	35.62455336951763	0.5234308209071733	3.882236245722261E-4
115-119	5.157828155031004E-4	5.656972815195295E-4	2.4957233008214536E-4	36.771388140711096	0.6295767059169994	3.882236245722261E-4
120-124	5.656972815195295E-4	5.823354368583391E-4	2.4957233008214536E-4	36.79591278168051	0.6418390264017022	3.882236245722261E-4
125-129	6.322499028747682E-4	5.878814886379423E-4	2.4957233008214536E-4	36.796328735563975	0.6419000329712778	4.048617799110358E-4
130-134	6.433420064339746E-4	6.100656957563553E-4	2.7175653720055824E-4	36.797277110418285	0.6419887697997515	4.658683494866713E-4
135-139	6.655262135523876E-4	6.100656957563553E-4	3.1612495143738416E-4	36.79912949171268	0.6420996908353436	5.213288672827036E-4
140-144	6.655262135523876E-4	6.100656957563553E-4	3.327631067761938E-4	36.8010817019391	0.642205065819156	5.601512297399262E-4
145-149	6.655262135523876E-4	6.100656957563553E-4	3.327631067761938E-4	36.80475873426897	0.6422660723887317	5.823354368583391E-4
150-154	7.043485760096103E-4	6.322499028747682E-4	3.882236245722261E-4	36.81048225970552	0.6423769934243238	5.823354368583391E-4
155-159	7.209867313484199E-4	6.766183171115941E-4	3.882236245722261E-4	36.81798052171155	0.642526736822373	5.934275404175456E-4
160-164	7.431709384668328E-4	7.043485760096103E-4	3.882236245722261E-4	36.82609994151689	0.6426210197026263	6.211577993155618E-4
165-169	7.48716990246436E-4	7.209867313484199E-4	4.2149993524984543E-4	36.83557259795644	0.6427097565310999	6.544341099931812E-4
170-174	7.48716990246436E-4	7.431709384668328E-4	4.658683494866712E-4	36.84580506348981	0.6428317696702511	6.821643688911972E-4
175-179	7.70901197364849E-4	7.598090938056425E-4	4.714144012662745E-4	36.857191107793334	0.6429815130683004	7.431709384668328E-4
180-184	7.764472491444522E-4	7.986314562628651E-4	4.714144012662745E-4	36.87220426996073	0.6431867169841458	7.653551455852456E-4
185-189	7.764472491444522E-4	8.041775080424683E-4	4.880525566050843E-4	36.887888504393445	0.6437302300585469	8.20815663381278E-4
190-194	7.764472491444522E-4	8.041775080424683E-4	4.991446601642907E-4	36.90318451520159	0.6449947298642964	8.596380258385006E-4
195-199	7.764472491444522E-4	8.374538187200878E-4	4.991446601642907E-4	36.91758761167322	0.6486717621941734	8.929143365161201E-4
200-204	8.041775080424683E-4	8.762761811773103E-4	4.991446601642907E-4	36.93388745785347	0.6667574370474595	9.650130096509621E-4
205-209	8.041775080424683E-4	9.42828802532549E-4	5.157828155031004E-4	36.94917792260984	0.7128950418019787	0.0010260195792265974
210-214	8.374538187200878E-4	9.705590614305651E-4	5.379670226215133E-4	36.96418553872545	0.7691874673649516	0.0010814800970226298
215-219	9.206445954141362E-4	9.705590614305651E-4	5.54605177960323E-4	36.983114213449234	0.8427447521178293	0.001109210355920646
220-224	9.42828802532549E-4	9.705590614305651E-4	5.54605177960323E-4	37.00539824949967	0.9491679397166355	0.001109210355920646
225-229	9.42828802532549E-4	9.705590614305651E-4	5.823354368583391E-4	37.026190397621406	1.0732386640781393	0.001109210355920646
230-234	9.483748543121524E-4	9.705590614305651E-4	5.823354368583391E-4	37.04543519729663	1.1773491480848512	0.001109210355920646
235-239	0.001014927475667391	9.927432685489783E-4	6.04519643976752E-4	37.069604890952135	1.2477673675304735	0.001109210355920646
240-241	0.0010260195792265974	0.0010260195792265974	6.100656957563553E-4	37.12452189567378	1.2493868146501177	0.001109210355920646
>>END_MODULE
