##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665057
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2517466
Total Bases	624.7 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.697884698343493	32.0	18.0	33.0	18.0	33.0
2	27.389982625386004	29.0	25.0	31.0	18.0	33.0
3	27.010095071790442	29.0	25.0	31.0	18.0	33.0
4	30.194612757431482	31.0	29.0	33.0	27.0	33.0
5	31.456685015805576	33.0	31.0	33.0	29.0	33.0
6	35.57138527392227	37.0	36.0	38.0	29.0	38.0
7	35.89543612505591	38.0	36.0	38.0	31.0	38.0
8	36.4330040604322	38.0	37.0	38.0	34.0	38.0
9	36.65259074005369	38.0	38.0	38.0	34.0	38.0
10-14	36.746437965795764	38.0	38.0	38.0	34.0	38.0
15-19	36.75258374889671	38.0	38.0	38.0	34.0	38.0
20-24	36.76556028959279	38.0	38.0	38.0	34.2	38.0
25-29	36.705285632457404	38.0	38.0	38.0	34.0	38.0
30-34	36.67829515870323	38.0	38.0	38.0	34.0	38.0
35-39	36.6464594159365	38.0	38.0	38.0	34.0	38.0
40-44	36.614330997916156	38.0	38.0	38.0	34.0	38.0
45-49	36.58930678706287	38.0	38.0	38.0	34.0	38.0
50-54	36.537355578983	38.0	38.0	38.0	34.0	38.0
55-59	36.45512082387607	38.0	38.0	38.0	34.0	38.0
60-64	36.405628358039394	38.0	38.0	38.0	33.8	38.0
65-69	36.33483177131289	38.0	37.2	38.0	33.2	38.0
70-74	36.249521622139085	38.0	37.0	38.0	33.0	38.0
75-79	36.139324840556995	38.0	37.0	38.0	33.0	38.0
80-84	36.01537943173445	38.0	37.0	38.0	32.4	38.0
85-89	35.87443959258436	38.0	37.0	38.0	31.0	38.0
90-94	35.767147244840096	38.0	36.4	38.0	31.0	38.0
95-99	35.650420045232536	38.0	36.0	38.0	31.0	38.0
100-104	35.52790308461333	38.0	36.0	38.0	29.0	38.0
105-109	35.36412689375945	38.0	35.4	38.0	28.8	38.0
110-114	35.21861375222626	38.0	35.0	38.0	28.6	38.0
115-119	35.071718780011494	38.0	35.0	38.0	28.0	38.0
120-124	35.114518658619176	38.0	35.0	38.0	28.8	38.0
125-129	33.13805066640441	34.2	32.8	35.6	28.8	35.6
130-134	34.275244920691875	36.0	34.8	36.8	28.6	37.2
135-139	35.50698333777005	38.0	36.8	38.0	29.0	38.0
140-144	35.411962211152826	38.0	36.0	38.0	28.4	38.0
145-149	35.373781842084576	38.0	36.0	38.0	28.0	38.0
150-154	35.347737297613264	38.0	36.0	38.0	28.0	38.0
155-159	35.33299691361276	38.0	36.0	38.0	28.0	38.0
160-164	35.306635693391605	38.0	36.0	38.0	28.0	38.0
165-169	35.229042553237136	38.0	36.0	38.0	27.4	38.0
170-174	35.17457358485439	38.0	36.0	38.0	27.0	38.0
175-179	35.09921747332625	38.0	36.0	38.0	27.0	38.0
180-184	34.99906959704181	38.0	36.0	38.0	27.0	38.0
185-189	34.876114916427426	38.0	35.2	38.0	26.6	38.0
190-194	34.72539166699117	38.0	35.0	38.0	25.0	38.0
195-199	34.57839389999299	38.0	35.0	38.0	25.0	38.0
200-204	34.36703771030081	38.0	34.4	38.0	23.2	38.0
205-209	34.186231531698795	38.0	34.0	38.0	23.0	38.0
210-214	34.0263092017666	38.0	34.0	38.0	22.2	38.0
215-219	33.79121301462107	38.0	34.0	38.0	21.0	38.0
220-224	33.61102270774175	38.0	34.0	38.0	19.8	38.0
225-229	33.40160950276339	38.0	33.0	38.0	15.0	38.0
230-234	33.202237476749204	38.0	33.0	38.0	15.0	38.0
235-239	33.04873477031264	38.0	32.0	38.0	15.0	38.0
240-244	33.04136717126922	38.0	31.4	38.0	16.0	38.0
245-249	33.498361187977125	38.0	32.4	38.0	21.8	38.0
250-252	35.28791090345435	37.333333333333336	33.333333333333336	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	6.0
17	8.0
18	22.0
19	81.0
20	429.0
21	1522.0
22	4042.0
23	8506.0
24	14169.0
25	21171.0
26	28905.0
27	38650.0
28	48324.0
29	59407.0
30	71729.0
31	86841.0
32	107351.0
33	138340.0
34	190638.0
35	282241.0
36	491144.0
37	923940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.19597614551743	16.893600551857432	11.610662356075201	22.299760946549938
2	18.245583650848634	18.79323795692808	35.01460199629469	27.946576395928602
3	21.52278210798353	25.954156931317225	28.05067651575518	24.47238444494407
4	14.826112223565287	18.73128319570106	37.32302081028593	29.119583770447715
5	17.154215543701444	35.01398435008026	32.477257804204804	15.354542302013499
6	30.634714897271497	35.49929869817248	19.475574795399016	14.390411609157002
7	26.376960006609824	29.04015386900955	24.533916247528268	20.04896987685236
8	25.376271218757275	32.12198297812165	20.828881105047696	21.67286469807338
9	24.277715876884088	15.979368134214377	19.82613462352009	39.916781365381446
10-14	25.53208713851442	21.706589157386887	26.90400997074494	25.857313733353752
15-19	25.218167792534242	25.986885225063617	24.170805087337826	24.624141895064323
20-24	25.06751638991448	25.030296376176842	24.89967279754839	25.00251443636029
25-29	25.2569597307209	24.957478254106253	24.685532929814034	25.100029085358816
30-34	25.087850218109104	24.85217146608391	24.910580708790437	25.149397607016542
35-39	24.918898241156626	24.69957610909381	25.198973700173156	25.18255194957641
40-44	24.682284812963783	24.498214597569767	25.460262937473647	25.3592376519928
45-49	24.229731345560342	24.448034678108453	25.88193092423976	25.440303052091444
50-54	23.91687707159244	24.61800790753364	25.713840138228903	25.751274882645014
55-59	23.7121737241024	24.47204189296923	25.6377313191892	26.17805306373917
60-64	23.78658991214568	24.535955444032346	25.2217569831215	26.455697660700473
65-69	23.769361731201137	24.645369589897143	24.88521394132036	26.700054737581365
70-74	23.92337434927058	24.714962897688057	24.73308445128595	26.628578301755407
75-79	23.968711893171662	24.740173448359666	24.744209311291907	26.546905347176768
80-84	23.89888553217775	24.66267436525387	24.88636241754706	26.552077685021324
85-89	23.866853003287638	24.28295623737739	25.193222283486964	26.656968475848007
90-94	23.87030831756882	23.94931031959381	25.40328722380386	26.777094139033515
95-99	23.647104645667465	23.866670459316364	25.69849642627518	26.78772846874099
100-104	23.53893654344957	24.11453643192113	25.764610634565503	26.581916390063796
105-109	23.253486296514183	24.99880600160413	25.521542870025666	26.226164831856018
110-114	22.981848811467444	25.781907562128904	25.26043185869067	25.97581176771298
115-119	22.958003872485726	25.969613936687715	25.170704089831116	25.90167810099545
120-124	23.00169270478193	26.01075834080738	25.146736996839387	25.84081195757129
125-129	24.670051813719375	23.843416284983828	26.31212852449501	25.17440337680179
130-134	23.535512576285676	29.124794605892763	25.933470069833	21.406222747988565
135-139	25.645781008100776	21.372330290637272	26.159142675717995	26.82274602554396
140-144	24.862958249939695	24.922265714362798	24.475807423634254	25.73896861206325
145-149	24.8351338888351	24.908115035784547	25.138768346760305	25.11798272862005
150-154	24.915380626737328	24.77641040803125	25.035600641897133	25.27260832333429
155-159	24.74889731616209	24.71286557470428	25.135683772537696	25.402553336595936
160-164	24.69108248884998	24.630098010996043	25.285848775417318	25.392970724736664
165-169	24.54788108910048	24.59674068724166	25.224531844668146	25.63084637898972
170-174	24.49939876444977	24.645708461743794	25.309007294012165	25.545885479794272
175-179	24.508856178873692	24.364897272220592	25.24917407628497	25.87707247262075
180-184	24.486085223555122	24.25881314944857	25.345845684584777	25.90925594241153
185-189	24.266445494239893	23.987765681567915	25.813548003444055	25.932240820748138
190-194	24.227978688672273	24.15924045651067	25.612135240934414	26.000645613882647
195-199	24.038358590709066	24.04781872089452	25.906246913872227	26.007575774524188
200-204	24.29042454060964	23.91377550159565	25.659449679146228	26.13635027864848
205-209	24.338469100157504	23.678165374376974	25.763176145882287	26.220189379583235
210-214	24.38432980171869	23.87860185686671	25.58334085116397	26.153727490250635
215-219	24.158424346080853	24.018397089998654	25.51022159486516	26.312956969055328
220-224	24.33404069161796	23.881879188974708	25.846429266886222	25.937650852521106
225-229	24.04412398825987	24.27404644144179	25.690030985212033	25.991798585086308
230-234	24.148945801411788	24.43811337949093	25.596466682098225	25.816474136999055
235-239	24.156519723441257	24.309064885027293	25.869532838173686	25.664882553357764
240-244	24.011328263412786	24.31381532180038	25.711373683336845	25.96348273144999
245-249	23.634569673050184	24.780128551634277	25.789119612503924	25.796182162811615
250-252	22.96113948223714	24.65937666847354	26.03973497337914	26.339748875910175
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.3333333333333333
13	2.333333333333333
14	4.333333333333333
15	6.999999999999997
16	15.666666666666677
17	21.99999999999999
18	44.33333333333337
19	74.00000000000004
20	130.99999999999932
21	207.33333333333485
22	310.83333333333314
23	459.16666666665816
24	675.1666666666731
25	949.6666666667038
26	1307.1666666666508
27	1730.4999999998877
28	2408.9999999999714
29	3252.8333333336845
30	4415.166666667146
31	5875.99999999916
32	7676.166666664199
33	9700.49999999965
34	12139.166666670719
35	14867.166666675643
36	17813.00000000712
37	21030.499999995453
38	24340.83333331687
39	28231.66666663616
40	32257.999999954933
41	36273.83333330932
42	40361.66666667206
43	44008.8333333651
44	46819.000000052
45	49104.33333340162
46	50643.833333412396
47	51277.16666675014
48	51347.833333416995
49	50807.50000007944
50	50077.166666740784
51	49416.833333402334
52	48515.333333395625
53	47554.66666672237
54	46455.50000004803
55	45132.833333371986
56	43681.833333361705
57	41901.0000000156
58	40230.83333333705
59	37900.99999998703
60	34880.999999965316
61	31612.99999995777
62	28144.999999970292
63	24465.49999998358
64	20436.333333331495
65	16761.666666678015
66	13304.333333339306
67	10153.500000000296
68	7491.666666664444
69	5387.833333332993
70	3755.50000000056
71	2489.4999999999905
72	1601.1666666665908
73	981.5000000000385
74	602.9999999999961
75	370.33333333333053
76	204.8333333333346
77	96.99999999999974
78	48.50000000000005
79	23.83333333333333
80	14.000000000000004
81	5.833333333333333
82	3.1666666666666665
83	0.8333333333333333
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03471745000726922
2	7.944496569169156E-5
3	3.1777986276676625E-4
4	3.5750234561261204E-4
5	3.5750234561261204E-4
6	1.986124142292289E-4
7	0.0
8	0.0
9	3.972248284584578E-5
10-14	8.421166363319306E-4
15-19	0.0
20-24	1.271119451067065E-4
25-29	0.0019146236731697667
30-34	0.002232403535936533
35-39	0.0028997412477467423
40-44	0.010899849292900082
45-49	0.005680315046955947
50-54	7.388381809327315E-4
55-59	1.1916744853753735E-4
60-64	1.1916744853753735E-4
65-69	0.0
70-74	8.659501260394381E-4
75-79	7.785659212957244E-4
80-84	0.002661463439437009
85-89	0.0021371580358453043
90-94	0.005712480602195563
95-99	0.0027490657546336457
100-104	0.0019625950050924175
105-109	0.0012237837119632726
110-114	3.497050952827167E-4
115-119	9.221254705920276E-4
120-124	9.542097787259089E-4
125-129	0.0011082599330387755
130-134	0.00231480834783747
135-139	0.001756061505256012
140-144	8.061918728675326E-4
145-149	0.0011334578806652441
150-154	0.0013409927433135549
155-159	0.001460724937831387
160-164	0.0016842288095878755
165-169	0.0015565223554924643
170-174	0.002139241642030798
175-179	0.0031290964622211893
180-184	0.0039435167068602025
185-189	0.0028901895629027405
190-194	0.00196459543469549
195-199	0.0011105941654815667
200-204	0.001023535806041244
205-209	0.001377298737617624
210-214	0.0030337940580334307
215-219	0.010490065738550984
220-224	0.011797366934654337
225-229	0.013183632222268357
230-234	0.025453929092862915
235-239	0.06877379365406604
240-244	0.029030537673581158
245-249	1.0386092606556117E-4
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	38.0
80-84	41.0
85-89	65.0
90-94	71.0
95-99	101.0
100-104	238.0
105-109	327.0
110-114	429.0
115-119	594.0
120-124	1546.0
125-129	8409.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	3.0
160-164	11.0
165-169	17.0
170-174	36.0
175-179	71.0
180-184	261.0
185-189	522.0
190-194	953.0
195-199	1556.0
200-204	2929.0
205-209	4396.0
210-214	8054.0
215-219	11868.0
220-224	17907.0
225-229	27765.0
230-234	43967.0
235-239	63039.0
240-244	110980.0
245-249	340189.0
250-253	1871082.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.57806440783067
#Duplication Level	Percentage of total
1	90.24287770233818
2	7.4067924877636155
3	1.2785780556288147
4	0.43402828616816147
5	0.21269551056747987
6	0.13762595804496244
7	0.08868620230310331
8	0.04005678402710639
9	0.04316476124252661
>10	0.11549425191609294
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.972248284584578E-5	0.0	0.0
5	0.0	0.0	0.0	3.972248284584578E-5	0.0	0.0
6	0.0	0.0	0.0	3.972248284584578E-5	0.0	0.0
7	0.0	0.0	0.0	3.972248284584578E-5	0.0	0.0
8	0.0	7.944496569169156E-5	0.0	3.972248284584578E-5	0.0	0.0
9	0.0	7.944496569169156E-5	0.0	3.972248284584578E-5	0.0	0.0
10-14	0.0	7.944496569169156E-5	0.0	1.0327845539919905E-4	3.1777986276676625E-5	0.0
15-19	7.944496569169156E-6	7.944496569169156E-5	7.944496569169156E-6	5.243367735651642E-4	7.944496569169156E-5	0.0
20-24	4.766697941501494E-5	1.1122295196836819E-4	3.972248284584578E-5	0.004290028147351345	1.0327845539919905E-4	3.972248284584578E-5
25-29	7.944496569169156E-5	1.4300093824504484E-4	7.944496569169156E-5	0.07151635811566075	1.1916744853753733E-4	3.972248284584578E-5
30-34	7.944496569169156E-5	1.986124142292289E-4	1.5094543481421397E-4	0.5602458980578089	5.402257667035026E-4	3.972248284584578E-5
35-39	8.738946226086072E-5	1.986124142292289E-4	2.2244590393673638E-4	1.525144728866249	6.276152289643634E-4	3.972248284584578E-5
40-44	1.3505644167587566E-4	1.986124142292289E-4	2.3833489707507466E-4	3.242713109134344	7.229491877943932E-4	3.972248284584578E-5
45-49	1.986124142292289E-4	2.3833489707507466E-4	2.3833489707507466E-4	5.128108979426138	8.818391191777764E-4	6.355597255335325E-5
50-54	1.986124142292289E-4	2.54223890213413E-4	2.3833489707507466E-4	7.066311918413199	0.0013585089133279257	7.944496569169156E-5
55-59	1.986124142292289E-4	3.0189086962842794E-4	2.3833489707507466E-4	8.965944326556944	0.002216514542798195	1.271119451067065E-4
60-64	1.986124142292289E-4	3.654468421817812E-4	2.7805737992092047E-4	10.860841814745461	0.0027805737992092046	1.5888993138338313E-4
65-69	1.986124142292289E-4	4.2900281473513444E-4	2.7805737992092047E-4	12.735075667357574	0.003487633993865259	1.6683442795255228E-4
70-74	1.986124142292289E-4	4.3694731130430365E-4	2.7805737992092047E-4	14.60752200824162	0.004123193719398792	2.4627939364424385E-4
75-79	1.986124142292289E-4	4.6078080101181107E-4	2.7805737992092047E-4	16.437489125970323	0.0048461429071931855	2.7805737992092047E-4
80-84	3.0983536619759715E-4	5.005032838576569E-4	2.7805737992092047E-4	18.26956947978642	0.005513480619003396	2.7805737992092047E-4
85-89	3.5750234561261204E-4	5.163922769959952E-4	3.1777986276676625E-4	20.07641811249884	0.0064270977244578475	3.098353661975971E-4
90-94	3.972248284584578E-4	5.481702632726718E-4	3.1777986276676625E-4	21.874456298516048	0.01092368278260759	3.5750234561261204E-4
95-99	3.972248284584578E-4	6.276152289643634E-4	3.1777986276676625E-4	23.658631338020054	0.0580583809274882	3.5750234561261204E-4
100-104	3.972248284584578E-4	6.355597255335325E-4	3.1777986276676625E-4	25.43195419521058	0.14526511976725803	4.210583181659653E-4
105-109	3.972248284584578E-4	6.514487186718707E-4	3.4955784904344293E-4	27.206484615879617	0.2877178877490302	4.3694731130430365E-4
110-114	4.131138215967961E-4	7.150046912252241E-4	3.5750234561261204E-4	28.94916554980286	0.4142498846061874	4.3694731130430365E-4
115-119	4.3694731130430365E-4	7.150046912252241E-4	3.5750234561261204E-4	29.970390861286706	0.5043801981834114	4.3694731130430365E-4
120-124	4.3694731130430365E-4	7.547271740710699E-4	3.5750234561261204E-4	29.992810230604896	0.514549153791948	4.3694731130430365E-4
125-129	4.3694731130430365E-4	8.023941534860848E-4	3.5750234561261204E-4	29.993088287984826	0.5145650427850863	4.6078080101181107E-4
130-134	4.925587872884878E-4	8.341721397627615E-4	3.813358353201195E-4	29.993922460124587	0.5145650427850863	4.7666979415014933E-4
135-139	5.322812701343334E-4	8.65950126039438E-4	3.972248284584578E-4	29.995336580513897	0.5145968207713628	4.7666979415014933E-4
140-144	5.561147598418409E-4	8.897836157469455E-4	3.972248284584578E-4	29.99731476015962	0.5146683212404854	4.925587872884878E-4
145-149	5.561147598418409E-4	9.13617105454453E-4	4.2900281473513444E-4	29.99980933208234	0.5146842102336238	5.481702632726718E-4
150-154	5.640592564110102E-4	9.295060985927913E-4	4.4489180787347276E-4	30.003344633055622	0.5146842102336238	5.958372426876868E-4
155-159	5.958372426876868E-4	9.771730780078064E-4	4.7666979415014933E-4	30.009017003606004	0.5147557107027463	6.037817392568559E-4
160-164	6.037817392568559E-4	0.0010089510642844828	4.7666979415014933E-4	30.015650658241263	0.5148033776821613	6.355597255335325E-4
165-169	6.355597255335325E-4	0.0010327845539919904	4.7666979415014933E-4	30.022594148242717	0.5148113221787305	7.308936843635624E-4
170-174	6.355597255335325E-4	0.0010883960299761743	5.005032838576569E-4	30.02974419515497	0.514930489627268	7.944496569169156E-4
175-179	6.355597255335325E-4	0.001159896499098697	5.402257667035026E-4	30.038157417021715	0.5150099345929597	7.944496569169156E-4
180-184	6.355597255335325E-4	0.0012313969682212192	5.561147598418409E-4	30.049065210811186	0.5151449910346356	7.944496569169156E-4
185-189	6.673377118102091E-4	0.0012313969682212192	5.720037529801794E-4	30.06093428868553	0.5155183823733865	8.341721397627615E-4
190-194	6.752822083793783E-4	0.0012631749544978958	6.991156980868859E-4	30.07157991408821	0.5163525545131493	8.341721397627615E-4
195-199	6.752822083793783E-4	0.0013108419339129106	7.150046912252241E-4	30.082797543243878	0.5186167360353625	8.341721397627615E-4
200-204	7.07060194656055E-4	0.0013505644167587567	7.150046912252241E-4	30.095111512926092	0.5323527706034561	8.341721397627615E-4
205-209	7.150046912252241E-4	0.0013505644167587567	7.308936843635624E-4	30.107822707436764	0.5667842187342351	8.341721397627615E-4
210-214	7.150046912252241E-4	0.0013505644167587567	7.547271740710699E-4	30.119350171958626	0.6078175435139939	8.738946226086073E-4
215-219	7.388381809327316E-4	0.0013505644167587567	8.023941534860848E-4	30.135286832076382	0.6624518464201701	9.13617105454453E-4
220-224	7.547271740710699E-4	0.0013902868996046023	8.421166363319306E-4	30.152907725466804	0.7399265769627077	9.374505951619605E-4
225-229	7.547271740710699E-4	0.0013902868996046023	8.818391191777764E-4	30.16878877410857	0.8288731605511256	9.533395883002987E-4
230-234	7.944496569169156E-4	0.0014220648858812792	9.13617105454453E-4	30.18366087168605	0.9067451159221218	9.930620711461446E-4
235-239	7.944496569169156E-4	0.0014300093824504481	9.13617105454453E-4	30.20377633699919	0.9606882476267803	9.930620711461446E-4
240-241	7.944496569169156E-4	0.0014300093824504481	9.13617105454453E-4	30.250140418976862	0.962297008182037	9.930620711461446E-4
>>END_MODULE
