##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665060
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8743
Total Bases	1.9 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.33077890884136	28.0	18.0	32.0	18.0	33.0
2	26.418620610774333	28.0	18.0	31.0	18.0	33.0
3	25.957566052842274	28.0	18.0	31.0	18.0	33.0
4	29.03877387624385	31.0	29.0	33.0	25.0	33.0
5	30.428914560219603	33.0	30.0	33.0	25.0	33.0
6	34.13244881619581	37.0	34.0	38.0	26.0	38.0
7	34.51160928742994	37.0	34.0	38.0	27.0	38.0
8	35.30790346562965	38.0	35.0	38.0	29.0	38.0
9	35.49136452018758	38.0	36.0	38.0	29.0	38.0
10-14	35.68088756719661	38.0	36.2	38.0	29.0	38.0
15-19	35.722131991307336	38.0	36.0	38.0	29.6	38.0
20-24	35.68621754546494	38.0	36.8	38.0	29.0	38.0
25-29	35.585268214571656	38.0	36.4	38.0	29.0	38.0
30-34	35.52419078119639	38.0	36.0	38.0	29.0	38.0
35-39	35.41024819855885	38.0	36.0	38.0	28.6	38.0
40-44	35.409950817797096	38.0	36.0	38.0	28.2	38.0
45-49	35.356559533340956	38.0	36.0	38.0	28.2	38.0
50-54	35.23193411872355	38.0	36.0	38.0	27.4	38.0
55-59	35.10355713141943	38.0	36.0	38.0	27.4	38.0
60-64	34.97177170307675	38.0	35.4	38.0	27.0	38.0
65-69	34.77282397346448	38.0	35.0	38.0	25.8	38.0
70-74	34.274276564108426	38.0	34.2	38.0	23.6	38.0
75-79	33.929776015267365	38.0	34.0	38.0	20.6	38.0
80-84	33.64063722575706	38.0	33.8	38.0	15.2	38.0
85-89	33.3619048789229	38.0	33.0	38.0	15.0	38.0
90-94	33.325576036414404	38.0	33.0	38.0	15.0	38.0
95-99	33.11691372262794	38.0	32.6	38.0	15.0	38.0
100-104	32.94343350213295	38.0	31.6	38.0	15.0	38.0
105-109	32.6136733916328	37.2	31.0	38.0	15.0	38.0
110-114	32.38426378734861	36.8	31.0	38.0	15.0	38.0
115-119	32.40769673721565	36.0	31.0	38.0	15.4	38.0
120-124	32.76771383409081	36.0	31.4	38.0	16.8	38.0
125-129	31.791087119730186	34.0	29.8	36.0	18.6	38.0
130-134	32.06737659734402	36.2	29.0	37.8	15.8	38.0
135-139	32.77754948634428	38.0	29.0	38.0	16.0	38.0
140-144	32.68611876722626	38.0	29.0	38.0	16.0	38.0
145-149	32.65319468804812	38.0	29.0	38.0	16.0	38.0
150-154	32.58361312954147	38.0	29.0	38.0	16.0	38.0
155-159	32.5370239786795	38.0	28.8	38.0	16.0	38.0
160-164	32.49181806791129	38.0	28.4	38.0	16.0	38.0
165-169	32.43321478731992	38.0	28.4	38.0	16.0	38.0
170-174	32.37091090558325	38.0	28.0	38.0	16.0	38.0
175-179	32.158915486293665	38.0	28.0	38.0	16.0	38.0
180-184	31.897398848144995	37.4	27.2	38.0	15.2	38.0
185-189	31.558912426512165	37.0	26.6	38.0	15.0	38.0
190-194	31.129597097649953	37.0	25.2	38.0	15.0	38.0
195-199	30.561230759247756	36.4	23.8	38.0	15.0	38.0
200-204	30.11190399298772	35.4	23.0	38.0	15.0	38.0
205-209	29.72825368852055	35.0	23.0	38.0	14.2	38.0
210-214	29.229766412792372	34.0	19.6	38.0	14.0	38.0
215-219	28.99561537844688	34.0	18.6	38.0	13.8	38.0
220-224	28.738463218423085	34.0	17.4	38.0	13.2	38.0
225-229	28.294387400551567	33.2	15.0	38.0	13.0	38.0
230-234	28.28167127291337	33.0	17.2	38.0	13.0	38.0
235-239	28.368362808028518	33.0	19.4	38.0	13.0	38.0
240-244	28.715096280971938	33.0	21.0	38.0	13.0	38.0
245-249	30.112805334636665	33.6	24.0	38.0	13.2	38.0
250-252	33.75402328291093	35.333333333333336	32.0	38.0	25.666666666666668	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	6.0
17	12.0
18	19.0
19	40.0
20	23.0
21	51.0
22	100.0
23	137.0
24	251.0
25	297.0
26	354.0
27	443.0
28	450.0
29	380.0
30	340.0
31	352.0
32	421.0
33	577.0
34	839.0
35	1126.0
36	1459.0
37	1066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17114746596499	20.36380276856195	13.236471799565267	22.228577965907792
2	14.869038087612948	20.13039002630676	37.02390483815624	27.976667047924053
3	19.66144344046666	25.803499942811392	32.72332151435434	21.811735102367606
4	13.851080864691752	20.073201418277478	39.43726409699188	26.638453620038888
5	14.525906439437264	35.61706508063593	34.38179114720348	15.47523733272332
6	25.883563994052384	39.76895802356171	19.92451103740135	14.422966944984559
7	23.172823973464485	32.059933661214686	25.952190323687525	18.815052041633308
8	22.738190552441953	34.713485073773306	23.081322200617638	19.467002173167106
9	22.864005490106372	17.900034313164817	23.51595562164017	35.720004575088645
10-14	22.347531683213614	24.660291897332662	29.290387518872674	23.70178890058105
15-19	22.349308017842844	28.81390826947272	26.57440237904609	22.26238133363834
20-24	22.013039002630677	27.935491250142974	27.015898433032138	23.03557131419421
25-29	22.194262707599396	28.279269799149017	27.37566912202041	22.150798371231183
30-34	22.277125797817583	27.083476311394776	28.01226179854963	22.627136092238008
35-39	20.636409388296656	26.14722972045569	29.544310747129067	23.67205014411859
40-44	19.895671272993503	25.183032854397364	30.310240688203532	24.6110551844056
45-49	18.750428953810253	24.80153737045595	30.781725423806368	25.666308251927433
50-54	18.344237544036236	25.433499565356637	29.38417898156197	26.838083909045157
55-59	18.426591023470742	25.708011163471657	26.968476918149793	28.896920894907808
60-64	19.74424669442284	27.05998078418813	23.64231138765613	29.5534611337329
65-69	21.16255661801711	28.443976758018024	21.338701560140915	29.054765063823947
70-74	21.50896778916545	29.83391288433382	20.27132137628111	28.385797950219622
75-79	22.54933382171146	29.581978847122386	20.832379469804497	27.036307861361657
80-84	22.670828838340945	27.869717905754353	21.890421649413547	27.56903160649115
85-89	22.1506168293132	25.38666912170871	24.392377094457743	28.070336954520346
90-94	22.34609791565681	22.957782240380396	25.75998892043487	28.93613092352792
95-99	21.943153171904996	24.357640093434167	26.3234579893152	27.375748745345636
100-104	20.706286085283	27.79697173464419	25.80749878266515	25.689243397407658
105-109	19.915115899444988	31.843197612051675	24.152324984842124	24.08936150366121
110-114	19.058132706987667	34.642395772166765	22.926600117439815	23.372871403405753
115-119	19.628578187840077	34.02413058907026	23.326236101253844	23.02105512183582
120-124	19.816207184628237	32.29979711182719	24.306003102995586	23.577992600548992
125-129	20.420064245119843	29.280948851000744	27.87991104521868	22.419075858660737
130-134	19.629657470745947	30.263850259339996	29.20393896113659	20.90255330877747
135-139	20.879478827361563	25.281884239538964	29.942370333249812	23.89626659984966
140-144	19.892257579553995	28.697068403908794	29.13304936106239	22.277624655474817
145-149	19.48634427461789	27.714858431470805	29.8371335504886	22.9616637434227
150-154	18.947632172387873	27.757454272112252	30.047607116011026	23.24730643948885
155-159	19.40762272283058	26.49159295361716	30.33803593354549	23.762748390006767
160-164	18.557036888532476	26.839414595028067	30.05212510024058	24.55142341619888
165-169	18.68685602547068	26.5060543006844	29.368998972147708	25.43809070169721
170-174	19.38058427868688	26.423049894588896	28.70695713281799	25.489408693906235
175-179	19.698568198944987	25.448379804069333	28.349660889223816	26.50339110776187
180-184	20.695468384369576	24.74649623833128	27.899252698588427	26.65878267871072
185-189	20.641530385624872	23.326771653543307	29.166666666666668	26.865031294165153
190-194	21.52389151958674	23.47623509153984	27.90509229951128	27.094781089362137
195-199	21.434929677763932	23.258475546173607	28.690963656247614	26.615631119814847
200-204	22.34162605796358	23.07771223390613	27.9148499615286	26.66581174660169
205-209	22.77534646243618	22.92643534437845	27.644055433989788	26.654162759195582
210-214	22.95086335536133	24.483052654018334	26.636111703261566	25.929972287358773
215-219	22.004620970403785	24.36461656947959	27.057432060732754	26.57333039938387
220-224	22.46372639336711	25.77153385536619	27.003684937816676	24.761054813450023
225-229	21.156657327323668	27.75612133024729	26.571445973931052	24.51577536849799
230-234	21.1287083072668	28.853182312074015	26.278357643804945	23.739751736854238
235-239	21.535677352637023	29.073718422218942	26.015659624759934	23.374944600384104
240-244	20.767023219410383	29.506913644664756	25.50656578832942	24.219497347595443
245-249	19.334637964774952	31.05954710651384	26.295778585406765	23.310036343304443
250-252	17.062597537714584	33.60499393098665	27.518640541009194	21.813767990289577
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.3333333333333333
11	0.6666666666666666
12	0.3333333333333333
13	0.0
14	0.0
15	0.3333333333333333
16	0.3333333333333333
17	1.3333333333333333
18	2.333333333333333
19	3.0
20	3.3333333333333335
21	4.5
22	10.500000000000002
23	12.000000000000004
24	16.00000000000001
25	18.833333333333336
26	21.16666666666666
27	25.666666666666643
28	28.666666666666632
29	35.49999999999997
30	48.00000000000006
31	57.00000000000012
32	60.00000000000014
33	64.33333333333351
34	84.16666666666659
35	89.99999999999984
36	97.49999999999973
37	106.99999999999963
38	134.99999999999932
39	151.6666666666663
40	157.33333333333312
41	179.83333333333368
42	202.50000000000068
43	200.6666666666672
44	208.00000000000085
45	214.0000000000008
46	223.1666666666676
47	216.00000000000085
48	208.5000000000006
49	196.83333333333366
50	172.33333333333306
51	164.16666666666606
52	137.49999999999955
53	133.4999999999996
54	122.33333333333312
55	109.99999999999994
56	104.83333333333329
57	98.16666666666669
58	76.33333333333347
59	81.33333333333348
60	82.33333333333343
61	69.83333333333337
62	62.33333333333338
63	57.33333333333332
64	50.16666666666663
65	36.999999999999986
66	34.00000000000001
67	36.33333333333334
68	31.333333333333336
69	22.166666666666664
70	20.166666666666668
71	14.999999999999996
72	8.666666666666664
73	5.833333333333333
74	2.333333333333333
75	2.5
76	2.833333333333333
77	1.3333333333333333
78	1.3333333333333333
79	0.5
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022875443211712226
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0022875443211712227
15-19	0.0
20-24	0.0
25-29	0.0022875443211712227
30-34	0.0045750886423424455
35-39	0.0022875443211712227
40-44	0.016012810248198558
45-49	0.009150177284684891
50-54	0.0022875443211712227
55-59	0.0022875443211712227
60-64	0.0022875443211712227
65-69	0.0022875443211712227
70-74	0.006862632963513668
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.00923211854040206
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0023487410747839157
115-119	0.007096728408203818
120-124	0.004773611475761988
125-129	0.0
130-134	0.0025056376847907794
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.002505951635133442
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.005032079506856209
185-189	0.0025236593059936906
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.002750501966608906
220-224	0.0057573838447809314
225-229	0.0
230-234	0.019751785890640944
235-239	0.08118680345412944
240-244	0.030428167789610952
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	20.0
80-84	24.0
85-89	28.0
90-94	17.0
95-99	17.0
100-104	37.0
105-109	60.0
110-114	58.0
115-119	66.0
120-124	100.0
125-129	334.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	1.0
160-164	1.0
165-169	9.0
170-174	7.0
175-179	7.0
180-184	22.0
185-189	24.0
190-194	33.0
195-199	44.0
200-204	100.0
205-209	145.0
210-214	212.0
215-219	294.0
220-224	352.0
225-229	433.0
230-234	593.0
235-239	761.0
240-244	888.0
245-249	1442.0
250-253	2614.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.29417819970261
#Duplication Level	Percentage of total
1	93.97232071371383
2	4.231956994166762
3	0.4117579778108201
4	0.04575088642342445
5	0.057188608029280566
6	0.0
7	0.0
8	0.0915017728468489
9	0.0
>10	0.24019215372297836
>50	0.9493308932860575
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	83	0.9493308932860575	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	11	0.12581493766441726	No Hit
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG	10	0.11437721605856113	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.022875443211712226	0.0	0.0
7	0.0	0.0	0.0	0.03431316481756834	0.0	0.0
8	0.0	0.0	0.0	0.03431316481756834	0.0	0.0
9	0.0	0.0	0.0	0.03431316481756834	0.0	0.0
10-14	0.0	0.0	0.0	0.043463342102253236	0.0	0.0
15-19	0.0	0.0	0.0	0.04575088642342445	0.0	0.0
20-24	0.0	0.0	0.0	0.08006405124099279	0.0	0.0
25-29	0.0	0.0	0.0	0.7022761065995654	0.0	0.0
30-34	0.0	0.0	0.0	4.334896488619467	0.057188608029280566	0.0
35-39	0.0	0.0	0.0	10.184147317854283	0.06862632963513668	0.0
40-44	0.0	0.0	0.0	18.025849250829236	0.08006405124099279	0.0
45-49	0.0	0.0	0.0	25.36429143314652	0.11666476037973235	0.0
50-54	0.0	0.0	0.0	30.80178428457051	0.27679286286171795	0.0
55-59	0.0	0.0	0.0	35.482099965686835	0.5101223836211826	0.0
60-64	0.0	0.0	0.0	39.44870181859774	1.1323344389797552	0.0
65-69	0.0	0.0	0.0	43.09504746654466	1.4640283655495825	0.0
70-74	0.0	0.0	0.0	46.13290632506005	1.550955049754089	0.0
75-79	0.0	0.0	0.0	48.919135308246595	1.612718746425712	0.0
80-84	0.0	0.0	0.0	51.13805329978268	1.6882077090243623	0.0
85-89	0.0	0.0	0.0	53.39128445613634	1.7614091273018413	0.0
90-94	0.0	0.0	0.0	55.45922452247513	1.8597735331122038	0.0
95-99	0.0	0.0	0.0	57.026192382477404	2.4705478668649206	0.0
100-104	0.0	0.0	0.0	58.48335811506348	3.3489648861946697	0.0
105-109	0.0	0.0	0.0	59.87876015097792	4.511037401349651	0.0
110-114	0.0	0.0	0.0	60.99508177970948	5.400892142285256	0.0
115-119	0.0	0.0	0.0	61.585268214571656	5.851538373555987	0.0
120-124	0.0	0.0	0.0	61.59213084753517	5.91330207022761	0.0
125-129	0.0	0.0	0.0	61.59213084753517	5.920164703191125	0.0
130-134	0.0	0.0	0.0	61.59213084753517	5.924739791833467	0.0
135-139	0.0	0.0	0.0	61.59213084753517	5.924739791833467	0.0
140-144	0.0	0.0	0.0	61.601281024819855	5.924739791833467	0.0
145-149	0.0	0.0	0.0	61.63101910099508	5.924739791833467	0.009150177284684891
150-154	0.0	0.0	0.0	61.65846963284913	5.924739791833467	0.011437721605856113
155-159	0.0	0.0	0.0	61.71108315223607	5.93160242479698	0.011437721605856113
160-164	0.0	0.0	0.0	61.76827176026535	5.936177513439323	0.011437721605856113
165-169	0.0	0.0	0.0	61.89408669792978	5.936177513439323	0.011437721605856113
170-174	0.0	0.0	0.0	62.003888825345996	5.936177513439323	0.011437721605856113
175-179	0.0	0.0	0.0	62.10911586411987	5.940752602081664	0.011437721605856113
180-184	0.0	0.0	0.0	62.20519272560905	5.947615235045179	0.011437721605856113
185-189	0.0	0.0	0.0	62.285256776850055	5.9567654123298635	0.011437721605856113
190-194	0.0	0.0	0.0	62.372183461054554	5.995653665789774	0.011437721605856113
195-199	0.0	0.0	0.0	62.50714857600366	6.029966830607344	0.011437721605856113
200-204	0.0	0.0	0.0	62.60093789317168	6.123756147775364	0.011437721605856113
205-209	0.0	0.0	0.0	62.6466887795951	6.2838842502573495	0.011437721605856113
210-214	0.0	0.0	0.0	62.726752830836105	6.558389568797895	0.011437721605856113
215-219	0.0	0.0	0.0	62.87315566739105	6.986160356856914	0.011437721605856113
220-224	0.0	0.0	0.0	62.93720690838385	7.423081322200618	0.011437721605856113
225-229	0.0	0.0	0.0	63.010408326661334	7.866864920507835	0.011437721605856113
230-234	0.0	0.0	0.0	63.122497998398714	8.184833581150635	0.011437721605856113
235-239	0.0	0.0	0.0	63.20713713828205	8.49365206450875	0.011437721605856113
240-241	0.0	0.0	0.0	63.262038201990165	8.498227153151092	0.011437721605856113
>>END_MODULE
