FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR5665066

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5665066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225584
Total Bases552.3 Mbp
Sequences flagged as poor quality0
Sequence length75-252
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCAAATTAAGATTTCCGCTTATGCTTGTCGCGCCTATGAAGGTATTT205440.9230835591916549No Hit
CGCTTATCAGAAGTTCTTGGAATTGAATATGCGCAAAGGTTATTCTTATC69590.3126819747086607No Hit
CGTAATTTGATAGACATTGCAACCTCTGGTATTTCTATCAATGATTTGCG63000.2830717690278147No Hit
GTTCAATCATATCGGATTCCAACCGATTTTGTACAAAGAAGTTTGCCCGG56990.25606762090309776No Hit
GGTTGTTACCTGCCCTATTTACGATTTATTCCAAAATCGTTCCTGTATAA54960.246946419456646No Hit
GGGTTTACTTGTAATCTGTATATGAATCTGTTTTCTTATTTTGGTCGTCG50380.22636755116859217No Hit
GCACACGGTTTGTTCCGGTCTAATTTGTCTAATTTCTTGATGCATCGTGT48310.21706662161482107No Hit
GACATTGTTGAGGGTCGTTTCAATGTTAAAGTGCGTTATGATGAGTTGTT38020.17083156600694469No Hit
GATATTAATGGTAGTGAAACATACGCCAAAGCTCTTGGTAGTCAATCAGG36730.16503533454589894No Hit
GCTTATCAGAAGTTCTTGGAATTGAATATGCGCAAAGGTTATTCTTATCG32330.14526524274078176No Hit
GGTTTCTGCTCAAGATGAGAATGGTGCTTCTGTTACTAGAGAATTATCTC31720.14252438910416323No Hit
GGTTCTAAGTCTGGTCGCCCTCACTATCATGCTATTTTTTGGAATTTTCC30130.13538019683822314No Hit
GAATAATAATAATGCTACACAAAGAACCGAGCAGACCTCAAGTTCAAATG28990.1302579457796246No Hit
GTTTTCTTATTTTGGTCGTCGTGTTTCAGGTAATAATACTGCTTGGGATT27740.1246414424258981No Hit
GTATTTATAACGCTTATATTCGTGACAATCGTAACAATCCTTATTATATC27020.12140633649415165No Hit
GATTTCCGCTTATGCTTGTCGCGCCTATGAAGGTATTTATAACGCTTATA24430.10976894154523038No Hit
GAATGGTGCTTCTGTTACTAGAGAATTATCTCGTGTTGCTCTTGTTGATG24280.10909496114278319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)