FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR5665069

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5665069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493591
Total Bases121.4 Mbp
Sequences flagged as poor quality0
Sequence length75-252
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGAAGGAACGCCGCTCGGGGTGCATTTTGCTTTAATGCATTAAAGA36000.7293487928264495No Hit
CTTCTATATAATGCGAATTTTGCGCCCGGTGCAATTCGTAAATTTAGAAT31980.6479048442941625No Hit
CCTCTTTACCTCTTTAATGCATTAAAGCAAAATGCACCCCGAGCGGCGTT15490.3138225778022695No Hit
CTATGAAACTGCTCCGGGATTTCCCGGGGCTTTTTCTATGCAAAAATTCA14580.29538626109471205No Hit
GCATTAAAGAGGTAAAGAGGTGAGTGCCGTGTCCGGCTGATTTTCTGAAA13920.28201486655956043No Hit
GAAAAAGCCCCGGGAAATCCCGGAGCAGTTTCATAGTCGAAAGTCGAAAG13560.274721378631296No Hit
CTTTAATGCATTAAAGCAAAATGCACCCCGAGCGGCGTTCCTTCTATATA13510.27370839419681475No Hit
TTCTAATTCTTATTATACGAATTGTCAACACCTAAATTCTAAATTTACGA13170.26682010004234274No Hit
TGTCAATACTTTTCAGATAAAAATTTATCTTTTTCGTGTTGGCTGTTTAT13140.266212309381654No Hit
GTAAAGAGGTGAGTGCCGTGTCCGGCTGATTTTCTGAAAATTTCCGTGAA12150.2461552175789267No Hit
GTTTCATAGTCGAAAGTCGAAAGTCGAAAGTCGCTCGGCTGATAGTCGGA11900.24109029540652077No Hit
TGCTATGACTATACAATAACACTTTCAAGACTATTTGTCAATACTTTTCA11820.2394695203113509No Hit
GAATTAGAATAATAACACAAACAAGATAAACAGCCAACACGAAAAAGATA11530.23359421059136004No Hit
AGACAAGACCGAGTACGTCCTCACTCCGAACCAGCAGAATGACAACGACT9800.19854494915831122No Hit
CAATAACACTTTCAAGACTATTTGTCAATACTTTTCAGATAAAAATTTAT9770.19793715849762253No Hit
CTTTCAAGACTATTTGTCAATACTTTTCAGATAAAAATTTATCTTTTTCG9310.18861770170039568No Hit
CAAGATAAACAGCCAACACGAAAAAGATAAATTTTTATCTGAAAAGTATT9210.18659173283143332No Hit
ACTATCAGCCGAGCGACTTTCGACTTTCGACTTTCGACTATGAAACTGCT9150.1853761515100559No Hit
CTATACAATAACACTTTCAAGACTATTTGTCAATACTTTTCAGATAAAAA8860.17950084179006506No Hit
TCCTCACTCCGAACCAGCAGAATGACAACGACTATTCCGCTGATGAAATC8780.17788006669489517No Hit
CGCTTCGACTTTCCGACTATCAGCCGAGCGACTTTCGACTTTCGACTTTC8690.17605669471282903No Hit
CGACTATTCCGCTGATGAAATCAGAGAACGCTACATCGCAAGCGACCAAC8680.1758540978259328No Hit
GGTGAGTGCCGTGTCCGGCTGATTTTCTGAAAATTTCCGTGAATTTTTGC8320.1685606098976683No Hit
CCTTCTATATAATGCGAATTTTGCGCCCGGTGCAATTCGTAAATTTAGAA8070.1634956877252624No Hit
ACTCTGACGAGGACACGAGCGACTAAGCGACTTTCACCCACGCTTCGACT7980.1616723157431963No Hit
GCTCCGGGATTTCCCGGGGCTTTTTCTATGCAAAAATTCACGGAAATTTT7840.15883595932664898No Hit
GTTTATCTTGTTTGTGTTATTATTCTAATTCTTATTATACGAATTGTCAA7740.15680999045768662No Hit
GGCTTTTTCTATGCAAAAATTCACGGAAATTTTCAGAAAATCAGCCGGAC7620.15437882781493178No Hit
TAATAACACAAACAAGATAAACAGCCAACACGAAAAAGATAAATTTTTAT7560.1531632464935544No Hit
GTCAACACCTAAATTCTAAATTTACGAATTGCACCGGGCGCAAAATTCGC7380.1495165025294221No Hit
ACTTTCACCCACGCTTCGACTTTCCGACTATCAGCCGAGCGACTTTCGAC7270.14728793677356355No Hit
TGACTATACAATAACACTTTCAAGACTATTTGTCAATACTTTTCAGATAA7030.14242561148805388No Hit
GGTAAAGAGGTGAGTGCCGTGTCCGGCTGATTTTCTGAAAATTTCCGTGA6910.13999444884529905No Hit
GAGGTAAAGAGGTGAGTGCCGTGTCCGGCTGATTTTCTGAAAATTTCCGT6900.1397918519584028No Hit
TCCCGGAGCAGTTTCATAGTCGAAAGTCGAAAGTCGAAAGTCGCTCGGCT6730.1363477048811668No Hit
ATATAATGCGAATTTTGCGCCCGGTGCAATTCGTAAATTTAGAATTTAGG6520.13209317025634584No Hit
TTATTATTCTAATTCTTATTATACGAATTGTCAACACCTAAATTCTAAAT6520.13209317025634584No Hit
GAATTGCACCGGGCGCAAAATTCGCATTATATAGAAGGAACGCCGCTCGG6360.12885162006600606No Hit
ACTTTCGACTTTCGACTTTCGACTATGAAACTGCTCCGGGATTTCCCGGG6290.12743344185773242No Hit
CCATTAAAAAGGCGTACTTTTTGCTCGAAAATTTGTGGGAATCCTACATG6290.12743344185773242No Hit
GTGCAATTCGTAAATTTAGAATTTAGGTGTTGACAATTCGTATAATAAGA6110.12378669789360017No Hit
GCTGATAGTCGGAAAGTCGAAGCGTGGGTGAAAGTCGCTTAGTCGCTCGT6060.12277371345911897No Hit
ATTCTAATTCTTATTATACGAATTGTCAACACCTAAATTCTAAATTTACG6010.1217607290246378No Hit
GTCGAAGCGTGGGTGAAAGTCGCTTAGTCGCTCGTGTCCTCGTCAGAGTT6010.1217607290246378No Hit
CTTTACCTCTTTAATGCATTAAAGCAAAATGCACCCCGAGCGGCGTTCCT6000.12155813213774157No Hit
GCTTTAATGCATTAAAGAGGTAAAGAGGTGAGTGCCGTGTCCGGCTGATT5940.12034255081636415No Hit
GAATAATAACACAAACAAGATAAACAGCCAACACGAAAAAGATAAATTTT5840.1183165819474018No Hit
CTATTTGTCAATACTTTTCAGATAAAAATTTATCTTTTTCGTGTTGGCTG5790.11730359751292062No Hit
CTCCGAACCAGCAGAATGACAACGACTATTCCGCTGATGAAATCAGAGAA5630.11406204732258084No Hit
GTGTTATTATTCTAATTCTTATTATACGAATTGTCAACACCTAAATTCTA5440.11021270647155236No Hit
GGACACGAGCGACTAAGCGACTTTCACCCACGCTTCGACTTTCCGACTAT5410.10960491581086364No Hit
CTCGTGTCCTCGTCAGAGTTGTTTGCTGAAAGTCGCTTCTGTTGGTCGCT5400.10940231892396743No Hit
CAACTATGGCTACCAAGACAAGACCGAGTACGTCCTCACTCCGAACCAGC5350.10838933448948623No Hit
CTACATCGCAAGCGACCAACAGAAGCGACTTTCAGCAAACAACTCTGACG5180.10494518741225023No Hit
CGTCAGAGTTGTTTGCTGAAAGTCGCTTCTGTTGGTCGCTTGCGATGTAG5140.10413479986466527No Hit
ATTCTAAATTTACGAATTGCACCGGGCGCAAAATTCGCATTATATAGAAG5110.10352700920397657No Hit
CAACAGAAGCGACTTTCAGCAAACAACTCTGACGAGGACACGAGCGACTA4980.1008932496743255No Hit
ACGCTACATCGCAAGCGACCAACAGAAGCGACTTTCAGCAAACAACTCTG4960.10048805590053304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)