##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665070
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	429777
Total Bases	107.2 Mbp
Sequences flagged as poor quality	0
Sequence length	154-252
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.407883623367468	32.0	28.0	33.0	18.0	33.0
2	29.24187194754489	31.0	28.0	33.0	18.0	33.0
3	31.087768773107914	33.0	31.0	33.0	27.0	33.0
4	31.38843167503147	33.0	31.0	33.0	29.0	33.0
5	32.14729033894322	33.0	33.0	33.0	31.0	34.0
6	35.81182101415385	38.0	36.0	38.0	31.0	38.0
7	36.06435663146236	38.0	36.0	38.0	33.0	38.0
8	36.53481922020024	38.0	37.0	38.0	34.0	38.0
9	36.69992810224838	38.0	38.0	38.0	34.0	38.0
10-14	36.82680646009442	38.0	38.0	38.0	35.0	38.0
15-19	36.82496364393627	38.0	38.0	38.0	35.0	38.0
20-24	36.825399218664565	38.0	38.0	38.0	35.0	38.0
25-29	36.766534737782614	38.0	38.0	38.0	34.8	38.0
30-34	36.74133376146234	38.0	38.0	38.0	34.2	38.0
35-39	36.72006924521322	38.0	38.0	38.0	34.0	38.0
40-44	36.72216381984145	38.0	38.0	38.0	34.0	38.0
45-49	36.67756953024475	38.0	38.0	38.0	34.0	38.0
50-54	36.61298115068978	38.0	38.0	38.0	34.0	38.0
55-59	36.54936955677014	38.0	38.0	38.0	34.0	38.0
60-64	36.522435123331405	38.0	38.0	38.0	34.0	38.0
65-69	36.50895417856237	38.0	38.0	38.0	34.0	38.0
70-74	36.43560823403765	38.0	38.0	38.0	34.0	38.0
75-79	36.37349695307101	38.0	37.8	38.0	33.8	38.0
80-84	36.24951591174028	38.0	37.0	38.0	33.0	38.0
85-89	36.18377344529838	38.0	37.0	38.0	33.0	38.0
90-94	36.099770346016655	38.0	37.0	38.0	33.0	38.0
95-99	36.02309430239403	38.0	37.0	38.0	32.2	38.0
100-104	35.86155099039735	38.0	37.0	38.0	31.0	38.0
105-109	35.76677858517324	38.0	36.8	38.0	31.0	38.0
110-114	35.665513510494975	38.0	36.0	38.0	31.0	38.0
115-119	35.53433385220707	38.0	36.0	38.0	31.0	38.0
120-124	35.55536755107882	38.0	35.6	38.0	31.0	38.0
125-129	32.99716411068996	34.4	32.8	35.6	28.4	35.6
130-134	33.9161220819169	36.0	34.6	36.4	27.6	37.2
135-139	35.13656617268956	38.0	36.0	38.0	28.0	38.0
140-144	35.052703146050156	38.0	36.0	38.0	27.0	38.0
145-149	35.02898945266964	38.0	36.0	38.0	27.0	38.0
150-154	34.99517982581665	38.0	36.0	38.0	27.0	38.0
155-159	34.98078437139239	38.0	36.0	38.0	27.0	38.0
160-164	34.96733407015299	38.0	36.0	38.0	27.0	38.0
165-169	34.94171575608751	38.0	36.0	38.0	27.0	38.0
170-174	34.86574998268887	38.0	36.0	38.0	25.2	38.0
175-179	34.81144599275136	38.0	35.8	38.0	25.0	38.0
180-184	34.73394989463606	38.0	35.0	38.0	25.0	38.0
185-189	34.633223257673066	38.0	35.0	38.0	25.0	38.0
190-194	34.58304599634516	38.0	35.0	38.0	25.0	38.0
195-199	34.482245845870565	38.0	35.0	38.0	25.0	38.0
200-204	34.413699729180806	38.0	35.0	38.0	24.2	38.0
205-209	34.293109164960086	38.0	34.2	38.0	23.0	38.0
210-214	34.20353247352606	38.0	34.0	38.0	23.0	38.0
215-219	34.02580943136604	38.0	34.0	38.0	23.0	38.0
220-224	33.93075185749233	38.0	34.0	38.0	21.8	38.0
225-229	33.753292910712105	38.0	34.0	38.0	21.0	38.0
230-234	33.6765815354593	38.0	34.0	38.0	21.0	38.0
235-239	33.495419334063946	38.0	33.0	38.0	20.8	38.0
240-244	33.49377757452525	38.0	33.0	38.0	21.0	38.0
245-249	33.908316512765445	38.0	33.0	38.0	22.8	38.0
250-252	35.45619740967821	37.333333333333336	33.666666666666664	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	45.0
21	203.0
22	627.0
23	1398.0
24	2429.0
25	3877.0
26	5285.0
27	6975.0
28	8688.0
29	10552.0
30	12303.0
31	14667.0
32	17277.0
33	21257.0
34	27582.0
35	39349.0
36	73000.0
37	184260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.24285235336848	22.10834621617091	12.18708674030062	24.461714690159997
2	16.516472496201516	20.240962173406206	40.57104033021776	22.67152500017451
3	17.51024368451546	29.15209515632526	29.864092308336648	23.473568850822634
4	12.718921673332915	19.481498544594057	40.3606986879242	27.438881094148826
5	13.039320391738041	37.21232173894834	37.37007797066851	12.378279898645111
6	27.49169918353006	40.898419412858296	19.63529923658083	11.974582167030809
7	24.067132489639974	31.550548307610693	27.022618706910794	17.35970049583854
8	22.702238602810297	37.896397434018105	20.93643913006047	18.464924833111123
9	23.661806006370746	16.6772535524237	21.16539507698178	38.49554536422377
10-14	23.418889330978622	23.862793960588863	29.340471919158073	23.377844789274437
15-19	22.306358879139644	28.358846564613742	26.853600820890833	22.481193735355777
20-24	22.750781802613453	27.43028926696698	26.913973790998103	22.904955139421464
25-29	22.90278821610478	27.362072535966664	27.179604116591783	22.555535131336775
30-34	22.812667965014413	27.44390695639832	26.895762220872687	22.84766285771458
35-39	22.42325671220191	26.94727730892998	27.535535870928413	23.093930107939702
40-44	22.255774506313738	27.1889840545213	27.663695358290465	22.8915460808745
45-49	22.02658588058458	26.652287116341732	27.7737989701636	23.54732803291009
50-54	21.43907189077126	26.888176891736876	28.261633358695327	23.411117858796537
55-59	21.662769296635233	26.65987244547754	28.14343252430912	23.533925733578112
60-64	21.696597683390493	26.15604770968387	27.80280431676773	24.344550290157898
65-69	21.282002955103373	26.686817214058845	27.395437041173665	24.63574278966412
70-74	21.25307451197576	26.49155425451956	27.086793734860137	25.168577498644538
75-79	20.692720229485797	26.759397320555994	27.17893302506883	25.36894942488938
80-84	21.264935070978673	26.491319917073277	26.869702194393835	25.37404281755422
85-89	20.946676997605735	26.01409568217936	27.133699569776887	25.90552775043802
90-94	20.876082247304996	26.14085909669433	27.18014225982312	25.80291639617755
95-99	21.0154103174437	25.59490154196246	27.263162058462875	26.12652608213096
100-104	20.723584556642166	25.629803363139487	27.762909601956366	25.883702478261984
105-109	20.686450616134916	25.860680912847624	27.499767320650758	25.953101150366702
110-114	20.307538809986845	26.862016122915005	27.18776918255062	25.642675884547533
115-119	20.16560354828847	27.10284070628848	27.209687709848957	25.521868035574098
120-124	20.126476893930757	27.52429088847947	26.873617575159937	25.47561464242984
125-129	22.302205161126338	24.969403150788814	28.56635431267769	24.162037375407163
130-134	20.618555741194797	32.31680719955421	28.407319286096346	18.65731777315464
135-139	23.7200101469209	23.327491871990542	28.5408681202829	24.411629860805657
140-144	22.012272714155788	27.00588222699867	27.350633416895278	23.631211641950266
145-149	22.468596715692822	27.364758414972485	27.39985134374734	22.766793525587353
150-154	22.729794564222765	26.95286864453718	27.5193087661845	22.79802802505555
155-159	22.55082525439972	27.217826109419224	27.174167519591673	23.05718111658938
160-164	22.196446233868997	26.622758532413282	27.861666104239152	23.319129129478572
165-169	22.08222771546807	27.17639354395189	27.630711143650654	23.11066759692939
170-174	21.98190080629097	26.976399559900287	27.377357577292045	23.664342056516695
175-179	21.96566712573532	26.48126436722762	27.98609110410588	23.566977402931183
180-184	22.15791597492848	26.556602917378186	27.858536805591434	23.42694430210191
185-189	22.28706408033605	25.501716333678537	28.326371030614737	23.88484855537067
190-194	21.52700458392758	26.197229907613938	28.304914968329562	23.970850540128914
195-199	21.33851479304264	26.151630316380626	28.38892788845586	24.120927002120872
200-204	21.249719462896	25.961951253127815	28.324422885122736	24.463906398853453
205-209	21.88453331594003	25.221578255886545	28.431061954323944	24.462826473849482
210-214	21.71103381566809	25.27644935951429	28.109827932135474	24.90268889268215
215-219	21.446929890968093	25.61203108147766	28.051743893708377	24.889295133845877
220-224	21.92659982613083	25.14772783754205	28.591866036336217	24.333806299990904
225-229	21.48512391746347	25.496643317677776	28.46214875752549	24.556084007333272
230-234	21.685828531565566	25.43903519562138	28.431255451507344	24.443880821305715
235-239	21.911977637258865	25.04412427095879	28.697642903676666	24.34625518810568
240-244	21.80798791162509	25.164238292983356	28.586268324419677	24.441505470971876
245-249	21.74189030963433	25.50972972887407	28.31675875158495	24.431621209906652
250-252	20.2312109585726	26.39969346650063	28.628489465431795	24.740606109494976
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.6666666666666666
19	2.333333333333333
20	4.333333333333333
21	6.333333333333331
22	7.33333333333333
23	15.333333333333343
24	24.666666666666647
25	39.0
26	61.33333333333349
27	115.99999999999943
28	185.66666666666757
29	301.00000000000034
30	493.99999999998937
31	786.3333333333528
32	1177.3333333333449
33	1672.6666666665656
34	2095.666666666502
35	2616.666666666739
36	3306.0000000003856
37	4344.666666667185
38	5876.999999999125
39	8125.1666666637475
40	10706.666666668261
41	12929.833333338971
42	14151.833333341194
43	14711.83333334221
44	15047.333333342822
45	14303.166666674804
46	13595.000000006845
47	12953.33333333901
48	12490.000000004833
49	11402.83333333619
50	10138.166666667219
51	8812.333333331482
52	6906.666666664855
53	5003.499999999922
54	3875.3333333339765
55	3060.6666666669403
56	2378.4999999999636
57	1808.8333333332014
58	1316.1666666666467
59	1055.3333333333726
60	878.5000000000298
61	721.0000000000119
62	673.83333333334
63	641.500000000003
64	605.3333333333322
65	533.9999999999908
66	514.833333333322
67	458.1666666666581
68	419.9999999999936
69	374.6666666666628
70	305.66666666666674
71	254.33333333333618
72	179.6666666666674
73	137.6666666666662
74	106.66666666666623
75	66.6666666666668
76	50.66666666666675
77	35.99999999999998
78	23.833333333333318
79	14.833333333333343
80	10.0
81	3.666666666666667
82	1.3333333333333333
83	1.0
84	0.3333333333333333
85	0.0
86	0.3333333333333333
87	0.3333333333333333
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06165988407941793
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	2.3267880784686012E-4
25-29	9.307152313874405E-4
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0025594668863154614
65-69	0.002792145694162321
70-74	0.007911079466793244
75-79	0.0037693966871191337
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	2.3267880784686012E-4
110-114	8.376437082486965E-4
115-119	8.376437082486965E-4
120-124	0.0015822158933586489
125-129	0.03667018011666515
130-134	0.034994892700167764
135-139	0.020941092706217412
140-144	0.017637053634791997
145-149	0.014146871517089094
150-154	0.017637053634791997
155-159	0.01800941515471344
160-164	0.020941190157640625
165-169	0.017218376009310952
170-174	0.01140145259159671
175-179	0.013262982132668914
180-184	0.011401617075061732
185-189	0.012472408287077607
190-194	0.010844225263880026
195-199	0.011217582466685874
200-204	0.005959603202169296
205-209	0.0
210-214	0.0013058592973917327
215-219	0.001588816229103562
220-224	0.002016241528854995
225-229	8.956729568049931E-4
230-234	7.613612949613585E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
154-155	2.0
156-157	0.0
158-159	0.0
160-161	0.0
162-163	0.0
164-165	1.0
166-167	1.0
168-169	3.0
170-171	0.0
172-173	1.0
174-175	1.0
176-177	1.0
178-179	1.0
180-181	3.0
182-183	1.0
184-185	7.0
186-187	13.0
188-189	11.0
190-191	11.0
192-193	7.0
194-195	18.0
196-197	25.0
198-199	27.0
200-201	81.0
202-203	102.0
204-205	96.0
206-207	152.0
208-209	154.0
210-211	211.0
212-213	240.0
214-215	346.0
216-217	485.0
218-219	594.0
220-221	591.0
222-223	750.0
224-225	930.0
226-227	1162.0
228-229	1461.0
230-231	1856.0
232-233	2208.0
234-235	2645.0
236-237	3185.0
238-239	3842.0
240-241	4921.0
242-243	6500.0
244-245	9116.0
246-247	15084.0
248-249	33756.0
250-251	173490.0
252-253	165685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.657433557237564
#Duplication Level	Percentage of total
1	19.820677296300897
2	4.57552220476636
3	2.5912411928265264
4	1.985341313655917
5	1.8062175241596918
6	1.6337968286896116
7	1.5509093149167938
8	1.495054237548842
9	1.403358818869788
>10	36.085870922032335
>50	16.641787572292195
>100	10.410222773941042
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	2.3267880784686012E-4	0.0	0.0
15-19	0.0	0.0	0.0	2.3267880784686012E-4	0.0	0.0
20-24	0.0	0.0	0.0	4.188218541243483E-4	0.0	0.0
25-29	0.0	0.0	0.0	0.008004150989931989	0.0	0.0
30-34	0.0	0.0	0.0	0.21992800917685215	0.0	0.0
35-39	0.0	0.0	0.0	0.9014442373603053	0.0	0.0
40-44	0.0	0.0	0.0	2.409482126777376	0.0	0.0
45-49	0.0	0.0	0.0	4.27579884451704	0.0	0.0
50-54	0.0	0.0	0.0	6.368744721099547	0.0	0.0
55-59	0.0	1.861430462774881E-4	0.0	8.595108626101444	0.0	0.0
60-64	0.0	2.3267880784686012E-4	0.0	11.040702503856652	0.0011168582776649287	0.0
65-69	0.0	2.3267880784686012E-4	0.0	13.42840589421956	0.0013960728470811608	0.0
70-74	0.0	2.3267880784686012E-4	0.0	16.005835584500797	0.0016287516549280206	0.0
75-79	0.0	2.3267880784686012E-4	0.0	18.60099539993997	0.0016287516549280206	0.0
80-84	0.0	2.3267880784686012E-4	0.0	21.33096931664561	0.0016287516549280206	0.0
85-89	0.0	2.3267880784686012E-4	0.0	24.032323739986087	0.0017218231780667648	0.0
90-94	0.0	2.3267880784686012E-4	0.0	26.77686335006294	0.0023733238400379733	0.0
95-99	0.0	2.3267880784686012E-4	0.0	29.511723521733366	0.022616380122714802	0.0
100-104	0.0	2.3267880784686012E-4	0.0	32.23406557354163	0.10228560392947972	0.0
105-109	0.0	2.3267880784686012E-4	0.0	34.95729180481971	0.2831235733880594	0.0
110-114	0.0	2.3267880784686012E-4	0.0	37.58390979507978	0.46382193556193096	0.0
115-119	0.0	2.3267880784686012E-4	0.0	39.13485365666381	0.599427144775081	0.0
120-124	0.0	2.3267880784686012E-4	0.0	39.17291990962755	0.6131086586764765	0.0
125-129	0.0	2.3267880784686012E-4	0.0	39.17324565995854	0.6131086586764765	2.3267880784686012E-4
130-134	0.0	2.3267880784686012E-4	0.0	39.17361794605109	0.6132482659611845	2.3267880784686012E-4
135-139	0.0	2.3267880784686012E-4	0.0	39.17473480432876	0.6133413374843233	2.3267880784686012E-4
140-144	0.0	2.3267880784686012E-4	0.0	39.17571205532171	0.6134809447690314	2.3267880784686012E-4
145-149	0.0	2.3267880784686012E-4	0.0	39.17794577187705	0.6135740162921701	3.722860925549762E-4
150-154	0.0	2.3267880784686012E-4	0.0	39.181296346710035	0.6137136235768782	4.6535761569372025E-4
155-159	0.0	2.3267880784686012E-4	0.0	39.186927173859935	0.613806695100017	4.6535761569372025E-4
160-164	0.0	2.3267880784686012E-4	0.0	39.192883751340815	0.613806695100017	4.6535761569372025E-4
165-169	0.0	2.7921456941623217E-4	0.0	39.20116711690016	0.613806695100017	4.6535761569372025E-4
170-174	0.0	4.6535761569372025E-4	0.0	39.20982276855206	0.613946302384725	4.6535761569372025E-4
175-179	0.0	5.118933772630923E-4	0.0	39.21973488576634	0.6140393739078639	4.6535761569372025E-4
180-184	0.0	6.980364235405804E-4	0.0	39.23206686258222	0.614178981192572	4.6535761569372025E-4
185-189	0.0	6.980364235405804E-4	0.0	39.2483543791315	0.6142720527157107	4.6535761569372025E-4
190-194	0.0	6.980364235405804E-4	0.0	39.26627064733571	0.6142720527157107	4.6535761569372025E-4
195-199	0.0	6.980364235405804E-4	0.0	39.28669984666467	0.61431858847728	4.6535761569372025E-4
200-204	0.0	6.980364235405804E-4	0.0	39.30931622678738	0.6155285182780837	4.6535761569372025E-4
205-209	0.0	6.980364235405804E-4	0.0	39.32834935326925	0.6217177745668103	4.6535761569372025E-4
210-214	0.0	6.980364235405804E-4	0.0	39.34538144200364	0.6320021778736414	4.6535761569372025E-4
215-219	0.0	6.980364235405804E-4	0.0	39.366741356563985	0.6456371560134675	4.6535761569372025E-4
220-224	0.0	6.980364235405804E-4	0.0	39.387449770462354	0.668346607659321	4.6535761569372025E-4
225-229	0.0	6.980364235405804E-4	0.0	39.406110610851684	0.6943600983766001	4.6535761569372025E-4
230-234	0.0	6.980364235405804E-4	0.0	39.428215097597125	0.7180468010154103	4.6535761569372025E-4
235-239	0.0	6.980364235405804E-4	0.0	39.45287905122889	0.7325194228634851	4.6535761569372025E-4
240-241	0.0	6.980364235405804E-4	0.0	39.512235415110624	0.7331709235254562	4.6535761569372025E-4
>>END_MODULE
