##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665076
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1346704
Total Bases	333.1 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.93779702146871	18.0	18.0	32.0	18.0	33.0
2	24.077699331107652	25.0	18.0	29.0	18.0	31.0
3	26.937704202259738	27.0	25.0	31.0	18.0	33.0
4	25.719488469626583	28.0	25.0	31.0	15.0	33.0
5	29.062897266214403	31.0	28.0	33.0	25.0	33.0
6	32.73080053226247	36.0	29.0	37.0	26.0	38.0
7	35.03474111608787	37.0	35.0	38.0	29.0	38.0
8	35.48118294740344	38.0	36.0	38.0	29.0	38.0
9	35.54474851192244	38.0	36.0	38.0	29.0	38.0
10-14	36.04895463294087	38.0	36.8	38.0	32.6	38.0
15-19	36.20573385094275	38.0	37.0	38.0	33.0	38.0
20-24	36.25050790671149	38.0	37.0	38.0	33.0	38.0
25-29	36.14807574641495	38.0	37.0	38.0	33.0	38.0
30-34	36.08579717591987	38.0	37.0	38.0	33.0	38.0
35-39	36.017854554527204	38.0	37.0	38.0	32.0	38.0
40-44	35.887379854815904	38.0	36.6	38.0	31.0	38.0
45-49	35.77894117786833	38.0	36.0	38.0	30.8	38.0
50-54	35.63471735437038	38.0	36.0	38.0	29.2	38.0
55-59	35.436827840416306	38.0	36.0	38.0	29.0	38.0
60-64	35.30011895709822	38.0	35.4	38.0	29.0	38.0
65-69	35.13940539272179	38.0	35.0	38.0	28.8	38.0
70-74	34.98411232163861	38.0	35.0	38.0	28.0	38.0
75-79	34.79186859543331	38.0	35.0	38.0	27.0	38.0
80-84	34.590383456227656	38.0	34.0	38.0	26.4	38.0
85-89	34.35345815617537	38.0	34.0	38.0	25.0	38.0
90-94	34.148434598621336	38.0	34.0	38.0	24.2	38.0
95-99	33.92602153920321	37.6	34.0	38.0	23.0	38.0
100-104	33.67995134802593	37.0	33.4	38.0	23.0	38.0
105-109	33.39383032528305	37.0	33.0	38.0	20.2	38.0
110-114	33.15326488803237	37.0	32.0	38.0	19.2	38.0
115-119	32.96520865036034	36.2	31.2	38.0	16.8	38.0
120-124	33.21228209870257	36.0	32.0	38.0	22.2	38.0
125-129	33.146918509923864	33.8	32.8	35.4	30.2	36.4
130-134	35.050430146704	36.2	35.4	37.2	31.8	38.0
135-139	36.11909650862575	38.0	37.0	38.0	33.0	38.0
140-144	36.07198663511484	38.0	37.0	38.0	32.4	38.0
145-149	36.040904598630746	38.0	37.0	38.0	31.8	38.0
150-154	36.00643615568085	38.0	37.0	38.0	31.0	38.0
155-159	35.988485965979166	38.0	37.0	38.0	31.0	38.0
160-164	35.9456611127462	38.0	37.0	38.0	31.0	38.0
165-169	35.86763986291798	38.0	37.0	38.0	31.0	38.0
170-174	35.799161317090665	38.0	37.0	38.0	31.0	38.0
175-179	35.71143388238072	38.0	37.0	38.0	29.2	38.0
180-184	35.61208995736264	38.0	36.0	38.0	29.0	38.0
185-189	35.49053499080584	38.0	36.0	38.0	29.0	38.0
190-194	35.36249444633976	38.0	36.0	38.0	29.0	38.0
195-199	35.227754760322746	38.0	36.0	38.0	28.2	38.0
200-204	35.04250023448105	38.0	35.8	38.0	27.2	38.0
205-209	34.88125122538294	38.0	35.0	38.0	26.4	38.0
210-214	34.727670534385084	38.0	35.0	38.0	25.6	38.0
215-219	34.461741596797665	38.0	34.2	38.0	24.2	38.0
220-224	34.259826382774754	38.0	34.0	38.0	23.0	38.0
225-229	34.02298400496064	38.0	34.0	38.0	23.0	38.0
230-234	33.745633419125426	38.0	33.6	38.0	21.4	38.0
235-239	33.51469044098103	38.0	33.0	38.0	21.0	38.0
240-244	33.34388436464504	38.0	32.2	38.0	21.0	38.0
245-249	33.687957394899044	38.0	32.8	38.0	22.4	38.0
250-252	35.285183227488915	37.333333333333336	33.333333333333336	38.0	30.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	7.0
17	13.0
18	23.0
19	49.0
20	136.0
21	521.0
22	1405.0
23	3043.0
24	5401.0
25	8777.0
26	13418.0
27	18832.0
28	25033.0
29	33241.0
30	42669.0
31	55618.0
32	71656.0
33	97777.0
34	139788.0
35	215393.0
36	364971.0
37	248932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.881596636782955	39.589030921096615	9.878261644619075	23.651110797501357
2	12.389294447253775	35.33629909490066	29.914019646499636	22.36038681134593
3	20.503778487164578	31.63422075988544	27.672099935917572	20.18990081703241
4	18.065021066278955	20.11514054334218	35.25984219225931	26.559996198119556
5	16.799720205153186	37.909528544198004	30.325885255895702	14.964865994753104
6	28.689441828587043	38.009030957874096	19.042014522897066	14.259512690641799
7	24.003641483206405	31.931070227756063	25.4362502821704	18.629038006867134
8	22.78496239708206	35.668639879290474	22.006840404424434	19.539557319203034
9	23.014057293998754	17.397377149972932	21.521746071702523	38.06681948432579
10-14	23.513590940096023	24.170991192832606	28.725551507961768	23.589866359109603
15-19	22.712771329111668	28.973805676674306	26.334398650334446	21.979024343879576
20-24	22.515171501116875	27.790397078018565	27.278554299961993	22.415877120902568
25-29	22.661567707250423	27.790492173344816	27.08110315739448	22.466836962010277
30-34	22.566334618575386	27.59613993722601	27.322027242854315	22.515498201344293
35-39	22.452783392467026	27.326582834942	27.489028459915076	22.7316053126759
40-44	22.190680265106906	26.991439410851097	27.884882302852994	22.932998021189007
45-49	21.739529498359907	26.79268883497986	28.349789712221046	23.117991954439184
50-54	21.3136741167179	26.872534887678945	28.252091864724644	23.56169913087851
55-59	21.001435208589392	26.583749114132942	28.157399492330605	24.25741618494706
60-64	21.106773935043744	26.499602882060426	27.630171221097566	24.763451961798264
65-69	21.04625812353717	26.43688590811344	27.154281861492947	25.362574106856446
70-74	21.010134582732046	26.328544278522294	26.824959281821474	25.836361856924185
75-79	21.037998325363613	26.05538775198629	26.77492001814239	26.131693904507706
80-84	20.938535561224427	25.87784699506792	26.76323884638554	26.42037859732211
85-89	20.914963853496232	25.393761776167633	26.987493312666587	26.703781057669545
90-94	20.968713154354162	24.86197504098788	27.141997344811227	27.02731445984673
95-99	20.82908638056836	24.499953804640867	27.49436372057125	27.17659609421952
100-104	20.701950312574088	24.676240269382358	27.55920963112039	27.06259978692317
105-109	20.47909157384647	25.550911780724554	27.27266244487911	26.697334200549864
110-114	20.19668933331986	26.440459767928814	26.982123636182088	26.380727262569238
115-119	20.09855460186527	26.821565786051348	26.8798721807305	26.20000743135288
120-124	20.27563448764928	27.06652631459988	26.77935872944403	25.878480468306808
125-129	21.999821361037707	26.17178794897976	28.366597620815803	23.46179306916674
130-134	21.98720112618598	30.26060707265863	26.998776613445763	20.753415187709635
135-139	23.46921522142093	23.797969447747963	28.428644277482874	24.304171053348234
140-144	22.301778816076784	27.550573808500268	27.186090158342925	22.961557217080024
145-149	22.175256656781603	27.23914347537171	27.89958084118156	22.686019026665132
150-154	22.22849838331909	27.108437250351148	27.82974522293947	22.833319143390295
155-159	22.1083595773878	26.90281053660569	27.99311277803523	22.995717107971284
160-164	21.990661456474623	26.88486029218673	28.04003558252925	23.084442668809398
165-169	21.915406288123904	26.75441595731484	28.00819814038021	23.32197961418104
170-174	21.88257493286886	26.66618782653717	28.044454320522117	23.406782920071855
175-179	21.851516206277438	26.280824010771966	28.120650040837802	23.747009742112798
180-184	21.856060436000003	26.00515406061858	28.244446739128808	23.89433876425261
185-189	21.561033608686124	25.598500187386776	28.822241193719613	24.01822501020749
190-194	21.438752164489042	25.695484554376858	28.61712372621308	24.248639554921024
195-199	21.27979314804393	25.57762294563966	28.895089362596828	24.24749454371958
200-204	21.616361739881064	25.082021508728182	28.559730481488586	24.741886269902167
205-209	21.71992763596098	24.850644861802035	28.654574576112964	24.774852926124026
210-214	21.807209207941785	25.068292555934583	28.25682846556224	24.867669770561392
215-219	21.427890163690353	25.093136250924864	28.252309417769006	25.226664167615777
220-224	21.54248428853149	24.91427929880246	28.834239538453794	24.708996874212257
225-229	21.361680637729876	25.246059613385253	28.598869034280238	24.793390714604637
230-234	21.586314807476903	25.295453765440378	28.430814303587752	24.687417123494964
235-239	21.89212728114992	24.999337536279864	28.662586315830136	24.445948866740075
240-244	21.698696292062632	24.74364516679683	28.794724569946585	24.762933971193952
245-249	21.447769688019026	25.75528672150475	28.341841993287225	24.455101597188996
250-252	19.961803682591515	26.189650061473717	29.37350264118302	24.475043614751748
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.6666666666666666
15	1.3333333333333333
16	1.6666666666666665
17	3.0
18	5.999999999999998
19	11.66666666666667
20	28.166666666666636
21	53.5000000000001
22	87.16666666666652
23	122.66666666666602
24	188.16666666666754
25	316.66666666666646
26	483.333333333324
27	709.3333333333424
28	1091.6666666667018
29	1608.666666666586
30	2321.6666666665915
31	3219.3333333336604
32	4485.166666667118
33	6018.666666665738
34	8052.666666663913
35	10810.500000001535
36	13824.500000006978
37	17374.33333334237
38	21505.666666660818
39	26132.33333331082
40	31135.49999995954
41	35910.83333330569
42	40038.00000000217
43	43248.50000002517
44	44933.50000003709
45	44929.00000003697
46	43299.33333335846
47	39961.50000000101
48	35599.666666636
49	30691.499999961732
50	26023.999999978365
51	21549.999999994394
52	17735.000000008124
53	14743.000000008304
54	12439.000000004216
55	10560.666666667557
56	9119.66666666498
57	7985.99999999741
58	7047.8333333315595
59	6241.833333332292
60	5539.166666666254
61	5003.166666666725
62	4531.333333333811
63	4060.0000000006676
64	3652.1666666671513
65	3164.166666666935
66	2689.5000000000596
67	2240.333333333195
68	1787.1666666665562
69	1412.3333333333057
70	1097.833333333377
71	784.000000000014
72	581.333333333326
73	415.166666666662
74	271.5000000000029
75	167.66666666666652
76	115.99999999999967
77	65.00000000000014
78	39.66666666666664
79	17.66666666666668
80	13.833333333333337
81	6.999999999999999
82	2.1666666666666665
83	0.3333333333333333
84	0.3333333333333333
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02777150732454942
2	7.425536717793962E-5
3	7.425536717793962E-5
4	1.4851073435587925E-4
5	2.2276610153381884E-4
6	2.2276610153381884E-4
7	0.0
8	0.0
9	7.425536717793962E-5
10-14	4.0097898276087395E-4
15-19	0.0
20-24	1.0395751404911548E-4
25-29	0.0017821288122705507
30-34	0.0023464696028228918
35-39	0.002821703952761706
40-44	0.01023238959712008
45-49	0.00543549287742518
50-54	8.019579655217479E-4
55-59	5.940429374235169E-5
60-64	5.940429374235169E-5
65-69	0.0
70-74	6.088940108591049E-4
75-79	5.494963272111027E-4
80-84	0.0020792478588202586
85-89	0.0017228591025032845
90-94	0.004307292335039615
95-99	0.0019012519446985222
100-104	0.001351765874150597
105-109	8.319504632924143E-4
110-114	1.7830566821832222E-4
115-119	5.499170888518876E-4
120-124	4.460951285965862E-4
125-129	7.169405279908519E-4
130-134	0.0014514037842735163
135-139	0.0011521452720521727
140-144	4.788136195541497E-4
145-149	5.685911732205528E-4
150-154	6.434062826379058E-4
155-159	7.481500866881505E-4
160-164	7.182292417160113E-4
165-169	8.379426413298748E-4
170-174	0.00110730553806894
175-179	0.001646055064432581
180-184	0.0022896434935290636
185-189	0.001646342321264846
190-194	9.131676378179546E-4
195-199	5.241356217394084E-4
200-204	4.7956800514096896E-4
205-209	9.154981683282958E-4
210-214	0.00353620517333274
215-219	0.00921943850596732
220-224	0.01008511349023113
225-229	0.01139529208575464
230-234	0.022599701883573634
235-239	0.05099167316826455
240-244	0.01923543292553222
245-249	1.812988759107096E-5
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	45.0
80-84	47.0
85-89	39.0
90-94	62.0
95-99	79.0
100-104	122.0
105-109	196.0
110-114	288.0
115-119	458.0
120-124	1518.0
125-129	7213.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	3.0
155-159	10.0
160-164	11.0
165-169	19.0
170-174	45.0
175-179	63.0
180-184	110.0
185-189	225.0
190-194	389.0
195-199	653.0
200-204	1568.0
205-209	2659.0
210-214	4877.0
215-219	7283.0
220-224	10493.0
225-229	16514.0
230-234	25277.0
235-239	35595.0
240-244	66449.0
245-249	232380.0
250-253	932014.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.18638510887419
#Duplication Level	Percentage of total
1	92.90666698897819
2	5.796812181269476
3	0.8973320897017627
4	0.20406434192643433
5	0.08442526708224858
6	0.03956233271696658
7	0.018632155495476768
8	0.011577228062109772
9	0.011983457037536874
>10	0.02894395772977751
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.425536717793962E-5	0.0	0.0
3	0.0	0.0	0.0	7.425536717793962E-5	0.0	0.0
4	0.0	0.0	0.0	1.4851073435587925E-4	0.0	0.0
5	0.0	0.0	0.0	2.2276610153381887E-4	0.0	0.0
6	0.0	0.0	0.0	2.2276610153381887E-4	0.0	0.0
7	0.0	0.0	0.0	2.970214687117585E-4	0.0	0.0
8	0.0	0.0	0.0	3.712768358896981E-4	0.0	0.0
9	0.0	0.0	0.0	3.712768358896981E-4	0.0	0.0
10-14	0.0	1.0395751404911548E-4	2.970214687117585E-5	5.049364968099894E-4	7.425536717793962E-5	0.0
15-19	0.0	1.4851073435587925E-4	7.425536717793962E-5	6.682983046014566E-4	7.425536717793962E-5	0.0
20-24	0.0	1.9306395466264304E-4	1.3365966092029132E-4	0.002910810393375233	7.425536717793962E-5	0.0
25-29	1.4851073435587925E-5	2.2276610153381887E-4	1.4851073435587925E-4	0.05494897171167532	1.4851073435587925E-4	0.0
30-34	7.425536717793962E-5	2.2276610153381887E-4	1.4851073435587925E-4	0.5338366857156436	9.80170846748803E-4	0.0
35-39	7.425536717793962E-5	2.2276610153381887E-4	1.4851073435587925E-4	1.6718595920113106	0.0011138305076690944	0.0
40-44	7.425536717793962E-5	2.2276610153381887E-4	1.4851073435587925E-4	3.8111567204077508	0.001321745535767325	0.0
45-49	7.425536717793962E-5	2.2276610153381887E-4	1.4851073435587925E-4	6.287721726526393	0.0016336180779146718	0.0
50-54	7.425536717793962E-5	2.8217039527617056E-4	1.9306395466264304E-4	8.933856289132578	0.0024355760434364195	0.0
55-59	1.188085874847034E-4	2.970214687117585E-4	2.8217039527617056E-4	11.631360714752462	0.004663237058774608	7.425536717793962E-5
60-64	1.4851073435587925E-4	2.970214687117585E-4	2.970214687117585E-4	14.410694555002436	0.006519621238223099	7.425536717793962E-5
65-69	1.4851073435587925E-4	2.970214687117585E-4	2.970214687117585E-4	17.194973802706464	0.007618600672456605	7.425536717793962E-5
70-74	1.4851073435587925E-4	2.970214687117585E-4	2.970214687117585E-4	20.026405208568477	0.009074005869144221	7.425536717793962E-5
75-79	1.4851073435587925E-4	3.2672361558293435E-4	2.970214687117585E-4	22.859499934655275	0.01023238959712008	7.425536717793962E-5
80-84	1.4851073435587925E-4	4.4553220306763774E-4	2.970214687117585E-4	25.687664104361467	0.011168007223562119	7.425536717793962E-5
85-89	1.4851073435587925E-4	4.4553220306763774E-4	2.970214687117585E-4	28.480705485392484	0.012059071629697393	7.425536717793962E-5
90-94	1.4851073435587925E-4	4.4553220306763774E-4	2.970214687117585E-4	31.252554384630923	0.014791669141845574	7.425536717793962E-5
95-99	1.4851073435587925E-4	4.4553220306763774E-4	3.267236155829343E-4	33.95586557996412	0.03757321579203744	7.425536717793962E-5
100-104	1.4851073435587925E-4	4.4553220306763774E-4	3.712768358896981E-4	36.61049495657546	0.0962795090829165	7.425536717793962E-5
105-109	1.4851073435587925E-4	4.4553220306763774E-4	4.752343499388136E-4	39.22024438926446	0.21003873159952002	1.4851073435587925E-4
110-114	1.4851073435587925E-4	4.4553220306763774E-4	5.197875702455774E-4	41.722278986325136	0.32039705829937387	1.4851073435587925E-4
115-119	1.4851073435587925E-4	4.4553220306763774E-4	5.197875702455774E-4	43.07121683755302	0.4104836697596502	1.4851073435587925E-4
120-124	1.4851073435587925E-4	4.4553220306763774E-4	5.197875702455774E-4	43.10111204837885	0.42396844443916404	1.4851073435587925E-4
125-129	2.0791502809823097E-4	4.4553220306763774E-4	5.79191863987929E-4	43.102567453575546	0.42407240195321316	1.6336180779146718E-4
130-134	2.2276610153381887E-4	4.4553220306763774E-4	6.385961577302807E-4	43.10485451888463	0.42407240195321316	2.376171749694068E-4
135-139	2.8217039527617056E-4	4.4553220306763774E-4	6.682983046014566E-4	43.10874550012475	0.4241466573203912	3.267236155829343E-4
140-144	2.970214687117585E-4	4.4553220306763774E-4	7.128515249082203E-4	43.11470078057241	0.4242357637610046	3.712768358896981E-4
145-149	2.970214687117585E-4	4.4553220306763774E-4	7.425536717793962E-4	43.12515593627107	0.42436942342192496	3.712768358896981E-4
150-154	2.970214687117585E-4	4.4553220306763774E-4	7.425536717793962E-4	43.14077926552532	0.42451793415628075	3.712768358896981E-4
155-159	2.970214687117585E-4	4.4553220306763774E-4	7.425536717793962E-4	43.16161532155543	0.4246664448906367	3.712768358896981E-4
160-164	2.970214687117585E-4	4.4553220306763774E-4	7.425536717793962E-4	43.18614929487103	0.42474070025781463	4.00978982760874E-4
165-169	2.970214687117585E-4	4.4553220306763774E-4	7.425536717793962E-4	43.213549525359696	0.42493376421247725	4.4553220306763774E-4
170-174	3.1187254214734637E-4	4.4553220306763774E-4	7.425536717793962E-4	43.243578395846455	0.4252604878280602	4.4553220306763774E-4
175-179	3.712768358896981E-4	4.6038327650322567E-4	7.425536717793962E-4	43.27709726859057	0.42531989212180255	4.4553220306763774E-4
180-184	3.712768358896981E-4	5.79191863987929E-4	7.425536717793962E-4	43.323893000986104	0.42542384963585167	4.900854233744015E-4
185-189	3.712768358896981E-4	5.94042937423517E-4	7.425536717793962E-4	43.371520393494045	0.42591393505922615	5.494897171167531E-4
190-194	3.712768358896981E-4	5.94042937423517E-4	7.425536717793962E-4	43.418509189844244	0.4265376801435208	5.94042937423517E-4
195-199	4.00978982760874E-4	5.94042937423517E-4	7.425536717793962E-4	43.46714645534579	0.4275772552840119	5.94042937423517E-4
200-204	4.4553220306763774E-4	5.94042937423517E-4	7.425536717793962E-4	43.52099644762323	0.432225641269351	5.94042937423517E-4
205-209	4.752343499388136E-4	5.94042937423517E-4	8.01957965521748E-4	43.573836566907055	0.44268079696800483	5.94042937423517E-4
210-214	5.197875702455774E-4	5.94042937423517E-4	8.168090389573359E-4	43.629023155793696	0.45653684848340836	5.94042937423517E-4
215-219	5.197875702455774E-4	5.94042937423517E-4	8.168090389573359E-4	43.69202140930746	0.4732888593187516	5.94042937423517E-4
220-224	5.197875702455774E-4	5.94042937423517E-4	8.316601123929239E-4	43.75873243118012	0.49667929997980254	5.94042937423517E-4
225-229	5.197875702455774E-4	6.237450842946927E-4	8.910644061352755E-4	43.82352766457959	0.5208271453860684	5.94042937423517E-4
230-234	5.197875702455774E-4	6.682983046014566E-4	8.910644061352755E-4	43.89459005096889	0.5372672836792644	5.94042937423517E-4
235-239	5.197875702455774E-4	6.682983046014566E-4	8.910644061352755E-4	43.98446874740106	0.5480046097731943	5.94042937423517E-4
240-241	5.197875702455774E-4	6.682983046014566E-4	8.910644061352755E-4	44.10490352742696	0.5488214188121517	5.94042937423517E-4
>>END_MODULE
