##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665086
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	212706
Total Bases	52.5 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.49591925004466	32.0	25.0	33.0	18.0	33.0
2	30.857305388658524	33.0	30.0	33.0	27.0	33.0
3	29.97843502298948	31.0	29.0	33.0	18.0	33.0
4	30.90374977668707	33.0	31.0	33.0	28.0	33.0
5	30.899307024719565	33.0	31.0	33.0	28.0	33.0
6	33.25196750444275	36.0	31.0	38.0	26.0	38.0
7	35.54312995402104	37.0	36.0	38.0	31.0	38.0
8	35.42124340639192	38.0	35.0	38.0	29.0	38.0
9	36.2644683271746	38.0	37.0	38.0	33.0	38.0
10-14	36.49783362951679	38.0	37.6	38.0	34.0	38.0
15-19	36.53782874013897	38.0	38.0	38.0	34.0	38.0
20-24	36.62271398079979	38.0	38.0	38.0	34.0	38.0
25-29	36.58079320752588	38.0	38.0	38.0	34.0	38.0
30-34	36.54500672289451	38.0	38.0	38.0	34.0	38.0
35-39	36.48575498575499	38.0	38.0	38.0	34.0	38.0
40-44	36.501725386213835	38.0	38.0	38.0	34.0	38.0
45-49	36.455845157165285	38.0	38.0	38.0	34.0	38.0
50-54	36.34228371555104	38.0	37.2	38.0	33.6	38.0
55-59	36.23423880849624	38.0	37.0	38.0	33.0	38.0
60-64	36.19424651866897	38.0	37.0	38.0	33.0	38.0
65-69	36.10958882213008	38.0	37.0	38.0	33.0	38.0
70-74	36.01759047699642	38.0	37.0	38.0	32.0	38.0
75-79	35.87005121073101	38.0	37.0	38.0	31.0	38.0
80-84	35.68665046228834	38.0	36.4	38.0	29.8	38.0
85-89	35.55057760242024	38.0	36.0	38.0	29.0	38.0
90-94	35.4327007650261	38.0	36.0	38.0	29.0	38.0
95-99	35.289728984437325	38.0	36.0	38.0	28.6	38.0
100-104	35.12696485086697	38.0	35.0	38.0	27.6	38.0
105-109	34.88869094306491	38.0	35.0	38.0	27.0	38.0
110-114	34.748350306982466	38.0	34.6	38.0	26.2	38.0
115-119	34.59691208448694	38.0	34.0	38.0	25.8	38.0
120-124	34.85313765775466	38.0	34.0	38.0	28.0	38.0
125-129	33.86939811858976	34.4	33.2	35.6	31.4	37.6
130-134	35.512102200572414	36.2	36.0	37.2	33.4	38.0
135-139	36.66854091245096	38.0	38.0	38.0	34.2	38.0
140-144	36.62559278985576	38.0	38.0	38.0	34.0	38.0
145-149	36.62061449231411	38.0	38.0	38.0	34.0	38.0
150-154	36.6228169601395	38.0	38.0	38.0	34.0	38.0
155-159	36.632613294729786	38.0	38.0	38.0	34.0	38.0
160-164	36.620755441195286	38.0	38.0	38.0	34.0	38.0
165-169	36.60043576300901	38.0	38.0	38.0	34.0	38.0
170-174	36.587266718178896	38.0	38.0	38.0	34.0	38.0
175-179	36.55229700929492	38.0	38.0	38.0	34.0	38.0
180-184	36.44553116848745	38.0	38.0	38.0	34.0	38.0
185-189	36.36780168995029	38.0	38.0	38.0	34.0	38.0
190-194	36.25449522205214	38.0	38.0	38.0	33.2	38.0
195-199	36.090950843331754	38.0	37.6	38.0	32.8	38.0
200-204	35.896473380949956	38.0	37.0	38.0	31.0	38.0
205-209	35.70901512077313	38.0	37.0	38.0	29.8	38.0
210-214	35.5046780968723	38.0	37.0	38.0	28.8	38.0
215-219	35.27322177844947	38.0	36.2	38.0	27.4	38.0
220-224	35.07160506421588	38.0	36.0	38.0	26.2	38.0
225-229	34.815152404659855	38.0	35.2	38.0	24.6	38.0
230-234	34.670101375766066	38.0	35.0	38.0	24.0	38.0
235-239	34.53738230983687	38.0	34.8	38.0	23.6	38.0
240-244	34.540964405134176	38.0	34.2	38.0	24.0	38.0
245-249	34.86337619231392	38.0	34.4	38.0	26.4	38.0
250-252	35.93057792330467	37.666666666666664	35.333333333333336	38.0	31.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	6.0
19	15.0
20	18.0
21	57.0
22	136.0
23	308.0
24	613.0
25	948.0
26	1468.0
27	2106.0
28	2681.0
29	3273.0
30	4019.0
31	5291.0
32	6982.0
33	9596.0
34	14397.0
35	23773.0
36	47136.0
37	89878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.7231236797659	24.50260400739566	14.875068805073463	24.899203507764973
2	13.455661805496788	21.48035316352148	45.063608924995066	20.000376105986668
3	13.81437289028048	30.32918676483033	34.52982050341787	21.326619841471324
4	10.017582954876685	20.736603574887404	47.48244055174749	21.76337291848843
5	10.595375776893928	38.03324776922137	40.531061653173865	10.840314800710841
6	21.074158697921074	46.54875743984655	22.145120494955478	10.231963367276899
7	19.98956305886999	35.15509670625182	30.70388235404737	14.151457880830817
8	19.09490094308576	40.332195612723666	24.195368254774195	16.377535189416378
9	21.226481622521227	18.303197841151636	26.350925690859683	34.119394845467454
10-14	19.768600791703104	26.598497456583264	33.499572179440165	20.133329572273468
15-19	19.454552292836123	31.995806418248662	29.713125158669712	18.836516130245503
20-24	19.32141077355599	30.347145825693683	30.813141143174143	19.518302257576185
25-29	19.397454305086594	30.480639310366225	30.849884487471336	19.272021897075845
30-34	19.30777693153931	30.364728780570367	30.893345744830892	19.434148543059436
35-39	19.093678598629094	29.69460193882636	31.225541357554558	19.986178104989985
40-44	18.803700882908803	28.917190864385585	32.00897012778201	20.270138124923605
45-49	18.026152603554024	28.809047631136774	32.35413863493828	20.810661130370924
50-54	17.597816316321964	29.30418119384236	31.485044061064915	21.612958428770764
55-59	17.347883852853702	28.53157413214507	31.0302741950498	23.090267819951425
60-64	18.11210121849968	28.344809364366103	29.68197148904441	23.861117928089808
65-69	18.353113969184708	28.25571839345255	28.597138875672545	24.794028761690196
70-74	18.85149475699561	28.15490066455586	27.876552659963117	25.117051918485412
75-79	18.64682992700867	27.619816929248625	27.975934114089153	25.75741902965355
80-84	18.580138832207115	27.225086088380934	28.28259557173781	25.91217950767414
85-89	18.868435529600948	26.10450491115719	28.824848715670093	26.202210843571766
90-94	19.066298997248122	25.29094099921563	28.991492315245175	26.65126768829107
95-99	18.862156604905874	24.7364866517297	29.74780225258528	26.653554490779147
100-104	18.850713988031682	25.407324551046923	29.569424623203034	26.17253683771836
105-109	18.464847815327058	27.765586860910002	28.711459817336987	25.058105506425953
110-114	17.964229130129038	29.761800536779564	27.943320897594848	24.33064943549655
115-119	17.84108835592893	30.20526754474776	28.009248576689256	23.944395522634053
120-124	17.82268065329569	30.41121217032729	28.17705172615256	23.58905545022446
125-129	18.980679313702574	28.855454921395268	31.317548353762458	20.8463174111397
130-134	17.379439386988288	35.2913018915855	31.406423580173424	15.922835141252792
135-139	20.106339623356806	26.686790950706563	32.22981490081603	20.977054525120604
140-144	19.214147207842796	30.91872691051563	30.155506314837737	19.711619566803837
145-149	19.051694316894835	30.470219732832927	31.083121277170438	19.3949646731018
150-154	19.112518007430435	30.126999772537722	31.076654788080976	19.683827431950867
155-159	18.924377744478342	29.878880418700888	31.204001997966017	19.992739838854753
160-164	18.787273326989208	29.792596327736092	31.191210982669915	20.228919362604785
165-169	18.900031941099474	29.564378375970435	30.935855258481215	20.599734424448872
170-174	18.901315540053833	29.653580771126357	30.600523183076167	20.84458050574364
175-179	19.45109807872953	29.312337022822256	30.00960208686618	21.22696281158203
180-184	19.651066895433818	29.14174400197169	29.81629112832131	21.390897974273173
185-189	19.81788082609743	28.53216193512052	30.385306069817407	21.264651168964647
190-194	20.14429023470402	28.95991895768043	29.52571651882261	21.37007428879294
195-199	20.353145738105724	28.605583058723482	29.8772987502088	21.16397245296199
200-204	20.88043204628253	28.104192691882034	29.152959393539255	21.862415868296182
205-209	21.566384859484945	27.267485287191175	29.155672697689244	22.01045715563464
210-214	21.693989540305893	28.18067924081819	28.191843117946842	21.933488100929083
215-219	22.09331668894341	28.87929586938312	27.610241097378317	21.417146344295155
220-224	22.387861672496815	29.036166281218406	27.959235456591554	20.61673658969323
225-229	22.010002984793264	29.7690908948096	27.43044646223451	20.790459658162625
230-234	22.258282582645023	29.909306686836363	27.45680039134029	20.375610339178326
235-239	22.628570123306048	29.867764750833736	27.145292649398005	20.358372476462208
240-244	22.61315198646406	29.328517662881374	27.45604616102127	20.602284189633295
245-249	21.78611965779896	30.37960583092567	27.34407245506735	20.490202056208023
250-252	20.755132721395242	31.469348707092514	27.809713471284375	19.965805100227875
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.3333333333333333
11	1.0
12	2.1666666666666665
13	6.499999999999998
14	11.333333333333336
15	19.0
16	35.166666666666636
17	49.166666666666735
18	69.33333333333343
19	107.99999999999956
20	157.00000000000003
21	228.16666666666868
22	310.66666666666674
23	419.9999999999938
24	574.999999999995
25	806.5000000000208
26	1059.333333333374
27	1327.166666666646
28	1701.6666666665622
29	2128.8333333331752
30	2440.8333333333144
31	2687.666666666753
32	3038.0000000002474
33	3421.5000000004247
34	3853.166666667286
35	4299.000000000591
36	4679.833333333587
37	5004.49999999995
38	5136.999999999827
39	5217.49999999977
40	5166.166666666485
41	4915.166666666717
42	4670.166666666955
43	4413.000000000527
44	4262.000000000662
45	3972.333333333967
46	3752.3333333338483
47	3581.333333333775
48	3345.500000000355
49	3082.5000000002483
50	2909.166666666837
51	2592.500000000031
52	2357.3333333332716
53	2062.999999999819
54	1718.8333333332298
55	1412.6666666666304
56	1070.5000000000396
57	833.6666666666903
58	579.999999999995
59	419.3333333333275
60	300.3333333333343
61	207.1666666666679
62	158.3333333333334
63	99.66666666666634
64	72.00000000000009
65	61.33333333333347
66	43.33333333333336
67	26.166666666666643
68	17.833333333333343
69	12.000000000000002
70	7.666666666666663
71	4.0
72	2.333333333333333
73	1.6666666666666665
74	1.5
75	0.8333333333333333
76	0.6666666666666666
77	0.6666666666666666
78	1.0
79	0.8333333333333333
80	0.5
81	0.0
82	0.0
83	0.3333333333333333
84	0.3333333333333333
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07004974001673671
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	8.462384700008463E-4
30-34	0.0
35-39	0.0
40-44	0.0
45-49	2.8207949000028207E-4
50-54	5.641589800005641E-4
55-59	0.0015984504433349317
60-64	8.462384700008463E-4
65-69	0.0030088478933363422
70-74	0.010624994123343959
75-79	0.00968484753887338
80-84	0.0022567717256593064
85-89	3.7615231912010446E-4
90-94	9.404825051444394E-4
95-99	1.8812268609096107E-4
100-104	0.0021636958182344995
105-109	1.8819561805322925E-4
110-114	9.413793655291353E-4
115-119	5.650829588873894E-4
120-124	0.0012250086457340958
125-129	0.02478701614467292
130-134	0.01819594761083417
135-139	0.006633939233116624
140-144	0.004359445781762354
145-149	0.003316969616558312
150-154	0.005686233628385678
155-159	0.008813779064147252
160-164	0.009572144112894436
165-169	0.007108048016285959
170-174	0.004454440081620506
175-179	0.007203415935662122
180-184	0.0019906137820619157
185-189	0.002749537082248135
190-194	0.0021815691552679677
195-199	0.0013287396904036522
200-204	0.0015199286393503826
205-209	9.515146209736658E-5
210-214	1.9083514229145296E-4
215-219	0.0
220-224	0.0
225-229	1.963675922780408E-4
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	8.0
80-84	14.0
85-89	16.0
90-94	28.0
95-99	29.0
100-104	43.0
105-109	64.0
110-114	106.0
115-119	105.0
120-124	185.0
125-129	1072.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	7.0
160-164	0.0
165-169	1.0
170-174	10.0
175-179	18.0
180-184	33.0
185-189	69.0
190-194	119.0
195-199	147.0
200-204	276.0
205-209	465.0
210-214	847.0
215-219	1662.0
220-224	2364.0
225-229	3652.0
230-234	5963.0
235-239	7241.0
240-244	10298.0
245-249	26421.0
250-253	151443.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.9938906130098
#Duplication Level	Percentage of total
1	73.3716274059143
2	12.79500328613136
3	5.420995573245529
4	2.797415548822927
5	1.5920789972065426
6	0.9323021039599408
7	0.6019742399930539
8	0.42602510501719215
9	0.2626440475941608
>10	1.0452941646655323
>50	0.36015817945563505
>100	0.3944813479938352
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAAAACAGTCTGCTGACTCTCCTGTTTTGAATTACTGTTTTTCCAAA	421	0.19792577548353127	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	8.462384700008462E-4	0.0	0.0
20-24	0.0	0.0	0.0	0.002820794900002821	0.0	0.0
25-29	0.0	0.0	0.0	0.05105638769005105	6.581854766673249E-4	0.0
30-34	0.0	0.0	0.0	0.9024663150075691	0.002444688913335778	0.0
35-39	0.0	0.0	0.0	3.068742771713069	0.0031969008866698637	0.0
40-44	0.0	0.0	0.0	7.131909772173799	0.003290927383336624	0.0
45-49	0.0	0.0	0.0	11.233815689261235	0.0036670333700036667	0.0
50-54	0.0	0.0	0.0	15.270749297151937	0.005171457316671838	0.0
55-59	0.0	0.0	0.0	19.010935281562343	0.007334066740007333	0.0
60-64	0.0	0.0	0.0	22.700158904779368	0.011941365076678607	0.0
65-69	0.0	0.0	0.0	26.170300790762838	0.018053087360018056	0.0
70-74	0.0	0.0	0.0	29.55008321344955	0.022378306206689045	0.0
75-79	0.0	0.0	0.0	32.74265888127274	0.026233392570026236	0.0
80-84	0.0	0.0	0.0	35.8337799591925	0.0346957772700347	0.0
85-89	0.0	0.0	0.0	38.78621195452879	0.044192453433377535	0.0
90-94	0.0	0.0	0.0	41.544949366731544	0.052466785140052465	0.0
95-99	0.0	0.0	0.0	44.23081624401757	0.140663639013474	0.0
100-104	0.0	0.0	0.0	46.768779442046764	0.4165373802337499	0.0
105-109	0.0	0.0	0.0	49.17651594219251	0.9417693906142752	0.0
110-114	0.0	0.0	0.0	51.48947373369815	1.4551540624147885	0.0
115-119	0.0	0.0	0.0	52.86827828081954	1.799573119705133	0.0
120-124	0.0	0.0	0.0	52.91895856252292	1.83680761238517	0.0
125-129	0.0	0.0	0.0	52.92027493347625	1.83680761238517	3.761059866670428E-4
130-134	0.0	0.0	0.0	52.922907675382916	1.8372777448685043	4.701324833338035E-4
135-139	0.0	0.0	0.0	52.92854926518294	1.8372777448685043	4.701324833338035E-4
140-144	0.0	0.0	0.0	52.936071384916275	1.8376538508551712	4.701324833338035E-4
145-149	0.0	0.0	0.0	52.94744859101295	1.8377478773518376	4.701324833338035E-4
150-154	0.0	0.0	0.0	52.968980658749636	1.8384060628285048	4.701324833338035E-4
155-159	0.0	0.0	0.0	52.99700055475633	1.8386881423185053	9.40264966667607E-4
160-164	0.0	0.0	0.0	53.0256786362397	1.8386881423185053	9.40264966667607E-4
165-169	0.0	0.0	0.0	53.06658016228973	1.8386881423185053	0.0014103974500014103
170-174	0.0	0.0	0.0	53.10879805929311	1.8386881423185053	0.0014103974500014103
175-179	0.0	0.0	0.0	53.15430688367983	1.8390642483051725	0.0014103974500014103
180-184	0.0	0.0	0.0	53.21335552358655	1.8394403542918394	0.0014103974500014103
185-189	0.0	0.0	0.0	53.27080571304994	1.8400045132718401	0.0014103974500014103
190-194	0.0	0.0	0.0	53.323554577679985	1.8409447782385076	0.0014103974500014103
195-199	0.0	0.0	0.0	53.37235432945003	1.8432014141585096	0.0014103974500014103
200-204	0.0	0.0	0.0	53.429992571906766	1.8524160108318526	0.0014103974500014103
205-209	0.0	0.0	0.0	53.48368170150347	1.8949159873252281	0.0014103974500014103
210-214	0.0	0.0	0.0	53.53323366524687	1.9644955948586311	0.0014103974500014103
215-219	0.0	0.0	0.0	53.57921262211691	2.0496836008387165	0.0016924769400016924
220-224	0.0	0.0	0.0	53.61654114129361	2.177935742292178	0.001880529933335214
225-229	0.0	0.0	0.0	53.66082762122365	2.3124876590223122	0.001880529933335214
230-234	0.0	0.0	0.0	53.70549020714037	2.4222165806324223	0.001880529933335214
235-239	0.0	0.0	0.0	53.768676012900436	2.4873769428224874	0.001880529933335214
240-241	0.0	0.0	0.0	53.840277190112175	2.4898216317358233	0.001880529933335214
>>END_MODULE
