##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665091
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5126115
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.394106257858045	30.0	18.0	32.0	18.0	33.0
2	30.44461097731908	31.0	29.0	33.0	27.0	33.0
3	31.105395021375838	33.0	31.0	33.0	27.0	33.0
4	31.40723901043968	33.0	31.0	33.0	29.0	34.0
5	31.743824514276405	33.0	32.0	33.0	29.0	34.0
6	34.46184020452136	37.0	34.0	38.0	28.0	38.0
7	35.44250255798007	38.0	35.0	38.0	29.0	38.0
8	35.52012176862985	38.0	36.0	38.0	29.0	38.0
9	36.33907744949148	38.0	37.0	38.0	33.0	38.0
10-14	36.489651831845364	38.0	37.6	38.0	33.8	38.0
15-19	36.42148196051005	38.0	37.2	38.0	33.4	38.0
20-24	36.514664419350716	38.0	38.0	38.0	33.8	38.0
25-29	36.492167850311596	38.0	38.0	38.0	34.0	38.0
30-34	36.46362553317668	38.0	38.0	38.0	34.0	38.0
35-39	36.41221427923485	38.0	38.0	38.0	33.6	38.0
40-44	36.43297276787587	38.0	38.0	38.0	34.0	38.0
45-49	36.379682039907415	38.0	37.4	38.0	33.4	38.0
50-54	36.25325424810016	38.0	37.0	38.0	33.0	38.0
55-59	36.13442909493837	38.0	37.0	38.0	33.0	38.0
60-64	36.108760377010654	38.0	37.0	38.0	33.0	38.0
65-69	36.04783938713822	38.0	37.0	38.0	32.6	38.0
70-74	35.96255413700239	38.0	37.0	38.0	31.4	38.0
75-79	35.88529798452001	38.0	37.0	38.0	31.0	38.0
80-84	35.81610444761543	38.0	37.0	38.0	31.0	38.0
85-89	35.70276015048536	38.0	36.0	38.0	31.0	38.0
90-94	35.61890944012805	38.0	36.0	38.0	30.8	38.0
95-99	35.505045140457625	38.0	36.0	38.0	29.0	38.0
100-104	35.406181360027304	38.0	36.0	38.0	29.0	38.0
105-109	35.25306046433071	38.0	35.0	38.0	28.2	38.0
110-114	35.156283831385394	38.0	35.0	38.0	28.0	38.0
115-119	35.03602927244519	38.0	35.0	38.0	28.0	38.0
120-124	35.158033021848766	38.0	35.0	38.0	28.8	38.0
125-129	33.334224339537485	34.4	33.2	35.6	30.6	35.6
130-134	34.799509753679786	36.2	35.4	36.6	31.8	37.2
135-139	36.13254653467205	38.0	37.0	38.0	32.8	38.0
140-144	36.04322496172151	38.0	37.0	38.0	31.8	38.0
145-149	36.02236136614891	38.0	37.0	38.0	31.2	38.0
150-154	36.0155110623848	38.0	37.0	38.0	31.2	38.0
155-159	36.01835314494894	38.0	37.0	38.0	31.2	38.0
160-164	35.98842819091053	38.0	37.0	38.0	31.0	38.0
165-169	35.96194270424855	38.0	37.0	38.0	31.0	38.0
170-174	35.93164643975	38.0	37.0	38.0	31.0	38.0
175-179	35.8957875392742	38.0	37.0	38.0	31.0	38.0
180-184	35.80046575134179	38.0	37.0	38.0	30.6	38.0
185-189	35.75225047696169	38.0	37.0	38.0	29.4	38.0
190-194	35.716671881134786	38.0	37.0	38.0	29.2	38.0
195-199	35.65469887501734	38.0	37.0	38.0	29.0	38.0
200-204	35.577151370799754	38.0	36.4	38.0	29.0	38.0
205-209	35.512118262145	38.0	36.2	38.0	29.0	38.0
210-214	35.43766875072659	38.0	36.0	38.0	29.0	38.0
215-219	35.30927201058763	38.0	36.0	38.0	28.0	38.0
220-224	35.20286904519074	38.0	35.8	38.0	28.0	38.0
225-229	35.04052531700032	38.0	35.0	38.0	27.0	38.0
230-234	34.93227527404495	38.0	35.0	38.0	26.8	38.0
235-239	34.83148354132494	38.0	35.0	38.0	25.8	38.0
240-244	34.807107295267556	38.0	35.0	38.0	26.0	38.0
245-249	34.9994867159937	38.0	35.0	38.0	28.2	38.0
250-252	35.905388077521174	37.666666666666664	34.333333333333336	38.0	31.666666666666668	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	13.0
19	85.0
20	422.0
21	1735.0
22	4839.0
23	10294.0
24	18187.0
25	28445.0
26	41057.0
27	56145.0
28	74042.0
29	93946.0
30	117538.0
31	146857.0
32	186553.0
33	243097.0
34	335675.0
35	516812.0
36	1013458.0
37	2236912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54678921939023	19.041379506938497	13.298946407394089	24.112884866277188
2	17.23416001607457	20.174459775541298	39.3483947866901	23.242985421694033
3	18.510605507880246	27.567278111472444	31.333994003250027	22.588122377397283
4	12.59832504569742	18.62597564234868	39.6671160002419	29.108583311712
5	13.842930830019093	34.90191687226437	36.98103689131976	14.274115406396778
6	28.143418553817074	39.52256240837359	18.86009190195694	13.473927135852396
7	23.065069745801644	30.88836282447819	26.918241202157972	19.128326227562198
8	22.947300245897722	36.15781152003027	20.696180245663626	20.198707988408376
9	23.525847547314097	14.735701403499531	20.72832934883435	41.01012170035202
10-14	24.18808395831931	22.386938256359837	29.215918878136755	24.209058907184094
15-19	23.707681158148034	27.480499364528498	25.460041376363975	23.3517781009595
20-24	23.612133062618657	26.260044262232206	26.46011504528465	23.667707629864488
25-29	23.423773621522137	26.46309262079491	26.57879560508915	23.5343381525938
30-34	23.684076537494768	26.245782624853327	26.430273218607077	23.639867619044832
35-39	23.717111301638766	26.165604946436044	26.24553292308113	23.871750828844064
40-44	23.36383791623871	26.22318461446924	26.651419252201713	23.761558217090332
45-49	23.535316513370947	26.0360170481808	26.525564272888563	23.903102165559687
50-54	23.435198585486543	26.111304913868143	26.519735608213985	23.933760892431327
55-59	23.598232566701398	25.969766635488554	26.44085224435383	23.991148553456217
60-64	23.522015591974878	25.893062955489693	26.444184316351695	24.14073713618374
65-69	23.0348989050617	26.076385512797483	26.40864232925934	24.480073252881475
70-74	23.138834466134337	25.723586566243956	26.513817838856124	24.623761128765583
75-79	22.928409569749746	25.841736248568104	26.350322402939348	24.8795317787428
80-84	22.729354823555926	26.002151248837098	26.40194423951777	24.866549688089208
85-89	22.901975846182285	25.66037828605076	26.39973166066295	25.037914207104002
90-94	22.87259652525621	25.701233586796963	26.279174042145026	25.146995845801808
95-99	22.710817765810905	25.554959683012278	26.494931821147976	25.239290730028834
100-104	22.541978150986264	25.165258986578028	26.656957936126364	25.635804926309348
105-109	22.3052880371219	25.694953875627586	26.53638104379499	25.463377043455527
110-114	22.068583237756062	26.026773809978103	26.359060417087587	25.54558253517825
115-119	22.23478466999837	25.7711191385044	26.41188331686341	25.582212874633814
120-124	22.19809548264937	25.72062721110921	26.50534549157625	25.57593181466517
125-129	24.1055482034991	23.905093499063828	27.7905857139757	24.198772583461373
130-134	20.932166310873818	31.505826621475148	27.61118418957213	19.95082287807891
135-139	24.514053436281642	22.08511091465841	28.087432040329247	25.3134036087307
140-144	23.386784580661036	26.670015838077983	25.459948026941987	24.483251554318997
145-149	23.628462118315877	26.414509939361825	26.439340934452904	23.517687007869394
150-154	23.4104830628533	26.48989563775413	26.423632714439897	23.67598858495267
155-159	23.51593083755963	26.47495706280056	26.311119242054847	23.697992857584964
160-164	23.67970264586492	26.145065779821003	26.370151122414047	23.805080451900036
165-169	23.307807683032163	26.505489881056622	26.355411952743157	23.831290483168065
170-174	23.618279680321752	26.300458821032414	26.22602241973077	23.855239078915066
175-179	23.680360933023792	26.205352921197395	26.176345629819153	23.937940515959664
180-184	23.85909760333112	26.17399624203522	26.113461773498354	23.85344438113531
185-189	23.792522326428195	26.06582053841644	26.249701926314035	23.89195520884133
190-194	23.35223486745588	26.136399426572737	26.464499166076568	24.04686653989481
195-199	23.33773018108356	26.051170324220045	26.457419649276794	24.153679845419603
200-204	23.32801995980317	26.01287740093346	26.355202770754783	24.303899868508584
205-209	23.3337211501844	25.84826584798164	26.52892748336407	24.289085518469893
210-214	23.4471475358339	25.974925427853467	26.224390335614316	24.353536700698317
215-219	23.69760765205097	26.15450833367702	25.888386106414078	24.259497907857934
220-224	23.79195799899454	25.958545614372973	26.359453810427812	23.89004257620467
225-229	23.535347319381074	25.92907030447759	26.252436638353092	24.283145737788242
230-234	23.596455349225113	26.13936953255374	26.10454050416471	24.159634614056436
235-239	23.60065918526023	26.418300052676713	26.138908507497828	23.842132254565232
240-244	23.822697876363723	25.859124796810185	26.164745041587313	24.15343228523878
245-249	23.791868957997398	26.031117355526685	26.20802577696545	23.96898790951047
250-252	23.508647173699934	25.67368256516739	26.51032218971679	24.30734807141589
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.3333333333333333
11	0.8333333333333333
12	1.0
13	2.0
14	5.666666666666666
15	12.66666666666667
16	19.166666666666668
17	42.66666666666669
18	86.8333333333332
19	162.50000000000014
20	298.5000000000007
21	501.1666666666558
22	839.6666666666907
23	1381.9999999999704
24	2175.999999999855
25	3416.000000000405
26	5206.666666666491
27	7222.833333331353
28	9686.999999999434
29	12797.16666667171
30	16302.000000011329
31	20154.999999999287
32	24043.49999998534
33	28351.66666663667
34	33145.999999952684
35	38853.49999999309
36	44643.16666670145
37	50244.50000007563
38	55176.66666677875
39	59863.6666668132
40	61950.16666682849
41	65409.00000018696
42	71715.33333343932
43	84174.83333325801
44	94087.16666644771
45	103119.3333329836
46	116789.1666661186
47	130546.66666591867
48	143111.66666623944
49	152473.99999984383
50	158669.5000000237
51	159027.1666667014
52	153492.49999987477
53	138759.99999944685
54	117919.9999994355
55	98287.16666638624
56	81831.3333332914
57	65980.50000018792
58	51907.16666675677
59	38431.33333332571
60	25499.833333312516
61	16107.166666677927
62	9869.166666666619
63	5460.999999999545
64	2880.8333333335117
65	2232.999999999888
66	1825.8333333332014
67	1264.4999999999948
68	869.0000000000277
69	560.8333333333263
70	285.8333333333348
71	135.99999999999943
72	83.49999999999991
73	52.16666666666675
74	27.166666666666647
75	12.66666666666667
76	8.999999999999996
77	4.833333333333334
78	6.833333333333333
79	5.666666666666666
80	2.333333333333333
81	0.5
82	0.0
83	0.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08585449214463585
2	9.753975476554857E-5
3	9.753975476554857E-5
4	9.753975476554857E-5
5	7.803180381243885E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	5.696321678308037E-4
25-29	0.0011470675160428511
30-34	0.0
35-39	0.0
40-44	0.0
45-49	6.125496599276451E-4
50-54	0.0010846420729929
55-59	0.0016152583389174842
60-64	0.0018220426190204474
65-69	0.003913294961193809
70-74	0.009785188198079832
75-79	0.011068822167296448
80-84	0.0027506324601395318
85-89	5.384237107444246E-4
90-94	0.0010339348740419569
95-99	1.9508339132187142E-4
100-104	0.0023761425919657897
105-109	3.8237675480203747E-4
110-114	3.1995554958998867E-4
115-119	3.62889754326758E-4
120-124	0.001416572761811134
125-129	0.02900836501021254
130-134	0.022118198966774194
135-139	0.010358859367530608
140-144	0.008250315108052134
145-149	0.007291885899198283
150-154	0.00924004032600834
155-159	0.012627798761083065
160-164	0.015154938710517355
165-169	0.012436153223578838
170-174	0.00950219900210089
175-179	0.012940882426567239
180-184	0.005911082222918999
185-189	0.0068931206773003555
190-194	0.005743176193173218
195-199	0.004765459200509317
200-204	0.003110991489031661
205-209	3.210048422406044E-4
210-214	9.087036062255128E-4
215-219	8.742337860309281E-4
220-224	0.00116200510261565
225-229	6.452805479155825E-4
230-234	3.6350073332321855E-4
235-239	7.960212628423603E-6
240-244	0.0
245-249	4.210601308814889E-6
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	14.0
80-84	18.0
85-89	23.0
90-94	31.0
95-99	43.0
100-104	89.0
105-109	134.0
110-114	150.0
115-119	268.0
120-124	1366.0
125-129	11447.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	3.0
160-164	11.0
165-169	8.0
170-174	18.0
175-179	40.0
180-184	61.0
185-189	139.0
190-194	283.0
195-199	533.0
200-204	1633.0
205-209	2230.0
210-214	3782.0
215-219	5854.0
220-224	9101.0
225-229	16333.0
230-234	29441.0
235-239	51062.0
240-244	115277.0
245-249	532751.0
250-253	4343971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.25655446722926
#Duplication Level	Percentage of total
1	13.694219333256896
2	5.847701523183451
3	3.1966678503922195
4	2.163803863877397
5	1.6922808473684807
6	1.4177132544742934
7	1.186307975015328
8	1.0908447043007796
9	0.9223212147714618
>10	15.63232669851333
>50	8.694902325220498
>100	27.0637456148548
>500	9.973557839902007
>1k	7.3244507405137576
>5k	0.09915621435530192
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.803180381243885E-5	0.0	0.0
6	0.0	0.0	0.0	9.753975476554857E-5	0.0	0.0
7	0.0	0.0	0.0	9.753975476554857E-5	0.0	0.0
8	0.0	0.0	0.0	9.753975476554857E-5	0.0	0.0
9	0.0	0.0	3.9015901906219425E-5	1.1704770571865828E-4	0.0	0.0
10-14	0.0	0.0	5.852385285932914E-5	1.4045724686238993E-4	0.0	0.0
15-19	0.0	0.0	6.242544304995108E-5	2.0288268991234103E-4	1.1704770571865827E-5	0.0
20-24	0.0	0.0	7.803180381243885E-5	0.0016698806015861916	3.9015901906219425E-5	0.0
25-29	0.0	1.9507950953109712E-5	7.803180381243885E-5	0.012781609464477487	3.9015901906219425E-5	0.0
30-34	0.0	1.9507950953109712E-5	7.803180381243885E-5	0.08092678373388033	1.6776837819674353E-4	0.0
35-39	0.0	1.9507950953109712E-5	7.803180381243885E-5	0.28697366329081575	1.7557155857798743E-4	0.0
40-44	0.0	1.9507950953109712E-5	7.803180381243885E-5	0.7754956726487798	1.989810997217191E-4	7.803180381243885E-6
45-49	0.0	1.9507950953109712E-5	7.803180381243885E-5	1.380831292313965	2.4970177219980433E-4	3.121272152497554E-5
50-54	0.0	1.9507950953109712E-5	7.803180381243885E-5	2.119585690137658	2.8871767410602374E-4	5.46222626687072E-5
55-59	0.0	1.9507950953109712E-5	7.803180381243885E-5	2.9557315822996557	3.628478877278407E-4	7.413021362181691E-5
60-64	0.0	1.9507950953109712E-5	8.19333940030608E-5	3.92961531296118	4.330765111590357E-4	7.803180381243885E-5
65-69	0.0	1.9507950953109712E-5	1.0144134495617051E-4	4.998662729962165	4.8769877382774283E-4	7.803180381243885E-5
70-74	0.0	1.9507950953109712E-5	1.1704770571865828E-4	6.214757179657498	6.398607912619986E-4	7.803180381243885E-5
75-79	0.0	3.121272152497554E-5	1.36555656671768E-4	7.509164347658998	7.491053165994129E-4	7.803180381243885E-5
80-84	0.0	3.9015901906219425E-5	1.36555656671768E-4	8.935507689546565	8.310387106024739E-4	9.753975476554857E-5
85-89	0.0	5.46222626687072E-5	1.36555656671768E-4	10.449968445889334	0.0010417245808960589	1.0534293514679245E-4
90-94	0.0	5.852385285932914E-5	1.36555656671768E-4	12.060985756269613	0.0019000744228328862	1.1704770571865828E-4
95-99	0.0	5.852385285932914E-5	1.36555656671768E-4	13.738349607841418	0.008169929859162348	1.1704770571865828E-4
100-104	0.0	5.852385285932914E-5	1.36555656671768E-4	15.525480797836178	0.028345052734868415	1.1704770571865828E-4
105-109	0.0	5.852385285932914E-5	1.36555656671768E-4	17.38152967695809	0.07519144615366609	1.1704770571865828E-4
110-114	0.0	5.852385285932914E-5	1.36555656671768E-4	19.30286776632986	0.12835061250089005	1.1704770571865828E-4
115-119	0.0	6.632703324057303E-5	1.36555656671768E-4	20.456205137809043	0.17143197138573754	1.4045724686238993E-4
120-124	0.0	8.973657438430468E-5	1.36555656671768E-4	20.483032471959756	0.17558326334855928	1.560636076248777E-4
125-129	0.0	9.753975476554857E-5	1.36555656671768E-4	20.483184633977196	0.17560667288970305	1.6776837819674353E-4
130-134	0.0	9.753975476554857E-5	1.36555656671768E-4	20.483328992814247	0.17565349197199046	1.911779193404752E-4
135-139	0.0	9.753975476554857E-5	1.36555656671768E-4	20.48391423134284	0.17567690151313423	2.184890506748288E-4
140-144	0.0	9.753975476554857E-5	1.36555656671768E-4	20.484866219349353	0.17570811423465918	2.6530813296229207E-4
145-149	0.0	9.753975476554857E-5	1.36555656671768E-4	20.48604840117711	0.1757276221856123	2.848160839154019E-4
150-154	0.0	9.753975476554857E-5	1.36555656671768E-4	20.487589529302404	0.17574322854637478	2.926192642966457E-4
155-159	1.9507950953109712E-5	1.1704770571865828E-4	1.36555656671768E-4	20.490016318400972	0.17582126035018722	3.082256250591335E-4
160-164	1.9507950953109712E-5	1.1704770571865828E-4	1.36555656671768E-4	20.493929613362166	0.17589148897361842	3.5114311715597486E-4
165-169	1.9507950953109712E-5	1.1704770571865828E-4	1.36555656671768E-4	20.498966566298257	0.17590319374419028	3.667494779184626E-4
170-174	1.9507950953109712E-5	1.1704770571865828E-4	1.36555656671768E-4	20.504495119598367	0.17591489851476214	3.940606092528162E-4
175-179	2.73111313343536E-5	1.1704770571865828E-4	1.36555656671768E-4	20.511931550501693	0.17598512713819334	4.0966697001530397E-4
180-184	3.9015901906219425E-5	1.1704770571865828E-4	1.36555656671768E-4	20.523460749514985	0.17610997802429326	4.0966697001530397E-4
185-189	3.9015901906219425E-5	1.1704770571865828E-4	1.36555656671768E-4	20.537108512001776	0.17630895912401498	4.564860523027673E-4
190-194	3.9015901906219425E-5	1.1704770571865828E-4	1.36555656671768E-4	20.55246517099207	0.17641430205916175	5.306162659245841E-4
195-199	3.9015901906219425E-5	1.1704770571865828E-4	1.36555656671768E-4	20.570045736391013	0.1770268517190894	5.618289874495597E-4
200-204	3.9015901906219425E-5	1.287524762905241E-4	1.36555656671768E-4	20.5891050044722	0.1777681538553076	5.696321678308038E-4
205-209	3.9015901906219425E-5	1.36555656671768E-4	1.36555656671768E-4	20.609658581596392	0.18109230869771747	6.008448893557791E-4
210-214	3.9015901906219425E-5	1.36555656671768E-4	1.36555656671768E-4	20.6304579589026	0.1853294356447329	6.242544304995108E-4
215-219	3.9015901906219425E-5	1.36555656671768E-4	1.36555656671768E-4	20.651975228803877	0.19151735768705933	6.242544304995108E-4
220-224	3.9015901906219425E-5	1.36555656671768E-4	1.36555656671768E-4	20.67119056049269	0.20271101994395363	6.242544304995108E-4
225-229	3.9015901906219425E-5	1.36555656671768E-4	1.36555656671768E-4	20.693983650386308	0.21718201796097042	6.242544304995108E-4
230-234	3.9015901906219425E-5	1.36555656671768E-4	1.36555656671768E-4	20.72273837009119	0.22921062051865787	6.242544304995108E-4
235-239	3.9015901906219425E-5	1.36555656671768E-4	1.36555656671768E-4	20.757919008840027	0.23718156927809853	6.359592010713765E-4
240-241	3.9015901906219425E-5	1.36555656671768E-4	1.560636076248777E-4	20.81204772034962	0.23747028695220454	6.437623814526206E-4
>>END_MODULE
