##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665094
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240892
Total Bases	58.3 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.3786261063049	30.0	18.0	32.0	18.0	33.0
2	28.875454560549954	31.0	27.0	33.0	18.0	33.0
3	30.765542234694387	33.0	30.0	33.0	27.0	33.0
4	30.290785912359066	33.0	30.0	33.0	25.0	33.0
5	31.738530129684673	33.0	32.0	33.0	29.0	34.0
6	34.65506118924663	37.0	34.0	38.0	28.0	38.0
7	35.219181209836776	37.0	35.0	38.0	29.0	38.0
8	35.723726815336335	38.0	36.0	38.0	31.0	38.0
9	36.28425601514372	38.0	37.0	38.0	33.0	38.0
10-14	36.52596765355429	38.0	38.0	38.0	34.0	38.0
15-19	36.462105009713895	38.0	38.0	38.0	33.4	38.0
20-24	36.48176527240423	38.0	38.0	38.0	33.8	38.0
25-29	36.42894077013766	38.0	38.0	38.0	33.2	38.0
30-34	36.37783238961858	38.0	38.0	38.0	33.0	38.0
35-39	36.3077545123956	38.0	38.0	38.0	33.0	38.0
40-44	36.33343739103	38.0	38.0	38.0	33.0	38.0
45-49	36.282020988658815	38.0	37.4	38.0	33.0	38.0
50-54	36.136290121714296	38.0	37.0	38.0	32.6	38.0
55-59	35.9792272055527	38.0	37.0	38.0	31.4	38.0
60-64	35.91620726300583	38.0	37.0	38.0	31.0	38.0
65-69	35.81768261295518	38.0	36.6	38.0	31.0	38.0
70-74	35.61686066785116	38.0	36.0	38.0	29.8	38.0
75-79	35.43764480151094	38.0	36.0	38.0	29.0	38.0
80-84	35.206537039687575	38.0	35.2	38.0	28.0	38.0
85-89	35.0298847920662	38.0	35.0	38.0	27.0	38.0
90-94	34.895482945322485	38.0	35.0	38.0	26.0	38.0
95-99	34.74822580823327	38.0	35.0	38.0	25.6	38.0
100-104	34.56295206141333	38.0	34.6	38.0	24.6	38.0
105-109	34.31815127507763	38.0	34.0	38.0	23.0	38.0
110-114	34.223583700477775	38.0	34.0	38.0	23.0	38.0
115-119	34.109775352390145	38.0	34.0	38.0	23.0	38.0
120-124	34.45963398377572	38.0	34.0	38.0	26.0	38.0
125-129	33.75136515068453	34.2	33.2	35.6	31.0	38.0
130-134	35.31785848807457	36.4	36.0	37.2	32.2	38.0
135-139	36.37988977257476	38.0	38.0	38.0	33.0	38.0
140-144	36.29632474229036	38.0	38.0	38.0	33.0	38.0
145-149	36.280208851963614	38.0	38.0	38.0	33.0	38.0
150-154	36.26992124757011	38.0	38.0	38.0	33.0	38.0
155-159	36.265609995800446	38.0	38.0	38.0	33.0	38.0
160-164	36.22155329860827	38.0	38.0	38.0	33.0	38.0
165-169	36.169879686251214	38.0	37.4	38.0	33.0	38.0
170-174	36.11209351169542	38.0	37.0	38.0	33.0	38.0
175-179	36.03627863558088	38.0	37.0	38.0	32.2	38.0
180-184	35.89368499139012	38.0	37.0	38.0	31.0	38.0
185-189	35.752379885012274	38.0	37.0	38.0	31.0	38.0
190-194	35.59990170259124	38.0	36.2	38.0	29.2	38.0
195-199	35.43039754935427	38.0	36.0	38.0	29.0	38.0
200-204	35.22114007640595	38.0	35.6	38.0	28.0	38.0
205-209	35.005219386191456	38.0	35.0	38.0	26.6	38.0
210-214	34.78268245552228	38.0	35.0	38.0	25.2	38.0
215-219	34.4880927941199	38.0	34.4	38.0	23.2	38.0
220-224	34.26986213202116	38.0	34.0	38.0	23.0	38.0
225-229	34.03040688008396	38.0	34.0	38.0	23.0	38.0
230-234	33.865234823722226	38.0	34.0	38.0	22.2	38.0
235-239	33.6830843227295	38.0	33.2	38.0	21.0	38.0
240-244	33.58397155492079	38.0	33.0	38.0	21.0	38.0
245-249	33.88949068899048	38.0	33.0	38.0	23.2	38.0
250-252	35.34650044975481	37.333333333333336	33.666666666666664	38.0	30.666666666666668	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	6.0
5	1.0
6	0.0
7	3.0
8	1.0
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	4.0
16	19.0
17	42.0
18	85.0
19	120.0
20	170.0
21	279.0
22	516.0
23	752.0
24	1150.0
25	1539.0
26	2079.0
27	2882.0
28	3807.0
29	4782.0
30	6031.0
31	7708.0
32	9867.0
33	13162.0
34	18414.0
35	28969.0
36	57171.0
37	81329.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.17686161925456	16.271069545599957	10.933105101561754	31.618963733583723
2	20.327991364278002	16.831769492651333	33.17611890724903	29.66412023582164
3	22.661014767731032	21.08667582816788	25.548963518680743	30.70334588542034
4	17.885611797528895	16.15766573668128	29.066195031220936	36.89052743456889
5	21.510657554948477	27.473823185060326	28.674925891174198	22.340593368817
6	35.84651087484484	29.232346799403846	15.824674003744649	19.096468322006668
7	31.12150851904746	25.200521438772462	19.21643030323159	24.46153973894849
8	28.91340177937568	28.218423969676714	18.334710280606632	24.53346397034097
9	26.810586403736377	13.687182148417229	18.625428126621692	40.876803321224706
10-14	29.339727840434023	18.501503279316022	22.42040839710819	29.73836048314177
15-19	29.377507022361126	21.749018364003664	20.213439923212775	28.660034690422435
20-24	28.953777148213867	21.002921881396027	20.876464780908716	29.166836189481394
25-29	29.09926516378129	20.98841698841699	20.759662888695146	29.15265495910657
30-34	28.787466143675367	20.656219018660625	21.38357991088999	29.172734926774023
35-39	28.155247099890314	20.1905424581372	22.39662689697605	29.257583544996436
40-44	27.067887677823105	20.046713723588606	23.563445058234393	29.321953540353903
45-49	25.559781891189708	20.37085472448692	24.521132923652782	29.54823046067058
50-54	24.612917877228107	21.1969965067743	24.001790205516592	30.188295410481004
55-59	24.347180602364922	20.809430457681426	23.640577139758857	31.20281180019479
60-64	24.674304560317385	21.17431369390524	22.218365818460807	31.933015927316568
65-69	24.455812787143604	21.454365496184984	21.652319692706	32.43750202396541
70-74	24.37922575737696	21.60955372016123	21.554414385387414	32.456806137074395
75-79	24.32178589460817	21.272936541681062	22.150537455821	32.25474010788977
80-84	23.89733240316258	21.060560760082385	22.707046043452262	32.33506079330277
85-89	23.76197734739395	20.349798768103604	23.747689675080057	32.14053420942239
90-94	23.86217085801275	19.32215530250151	24.363819476297788	32.45185436318795
95-99	23.183240850427083	19.45607581520045	25.413221938192322	31.947461396180145
100-104	22.595645143043473	21.213873120869906	25.296967438393814	30.893514297692803
105-109	21.6876946638159	24.4168626150485	24.41619602948308	29.479246691652516
110-114	21.279018842202312	26.694697035039077	23.49878284782625	28.52750127493236
115-119	21.394489062955007	26.874870751687084	23.195651348191788	28.534988837166118
120-124	22.090010457625343	26.351258786660186	22.96516649237291	28.593564263341552
125-129	26.415312394618617	21.420741557199396	22.674321829028383	29.489624219153605
130-134	28.211672415779038	23.633918232985845	21.419867650612165	26.734541700622948
135-139	29.699783332727147	18.208450889617463	21.72344873333645	30.36831704431894
140-144	28.828643154723505	20.641692984605843	20.71105630552421	29.818607555146436
145-149	28.680043921177422	20.702184628280666	21.209113990696192	29.40865745984572
150-154	28.401265700014548	20.49176636854247	21.637183315205718	29.46978461623726
155-159	27.976026041932776	20.26970269832596	22.122999463954628	29.631271795786628
160-164	27.540111351380375	20.61186968649624	22.335659720989362	29.51235924113402
165-169	27.372633515166893	20.651864219779878	22.181240630837458	29.79426163421577
170-174	27.310335271729013	20.931642324862416	22.232460261445265	29.525562141963306
175-179	27.41172231979852	20.426500798508986	22.495435854266045	29.666341027426448
180-184	27.510531639956486	20.545532021035566	22.362239913529695	29.581696425478256
185-189	27.09000144659258	20.219639529918044	23.70488818359079	28.985470839898582
190-194	26.900351228607022	20.949478182067068	23.454776067937697	28.695394521388213
195-199	26.681076846008107	20.852841099171965	24.125552298724056	28.340529756095872
200-204	27.07933454355902	20.688267431543174	23.792036622481575	28.44036140241623
205-209	27.18692941264356	20.481035000946818	24.065908865852577	28.26612672055704
210-214	27.49293056378372	21.40156753460119	23.101722228959865	28.00377967265522
215-219	27.209473773110844	22.072128343901976	23.26444361553133	27.453954267455856
220-224	27.161203313595994	22.39639674903211	24.364200210658165	26.07819972671373
225-229	26.490794472433155	23.68297065571354	23.728096234139354	26.09813863771395
230-234	26.852854682690218	23.987768697600366	23.78555465352384	25.373821966185577
235-239	27.20130816194382	23.69097083037834	23.81334602694952	25.294374980728318
240-244	26.7472008238513	23.202923864427998	24.32966167577009	25.72021363595061
245-249	26.233625284975464	24.584600350518443	24.0416166201772	25.14015774432889
250-252	25.850342059139482	25.34743031605134	22.621982246847967	26.180245377961214
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	2.0
3	3.5
4	2.5
5	1.0
6	1.0
7	1.5
8	1.0
9	1.0
10	1.1666666666666665
11	1.1666666666666665
12	2.333333333333333
13	3.333333333333333
14	3.6666666666666665
15	4.833333333333333
16	4.666666666666667
17	4.833333333333333
18	9.833333333333332
19	15.499999999999998
20	28.5
21	43.3333333333333
22	44.66666666666663
23	62.16666666666671
24	86.83333333333346
25	118.33333333333304
26	156.4999999999993
27	207.66666666666734
28	277.16666666666924
29	328.5000000000004
30	382.666666666664
31	460.83333333332695
32	559.4999999999885
33	646.1666666666636
34	713.1666666666696
35	803.6666666666773
36	925.8333333333557
37	1065.8333333333685
38	1197.0000000000439
39	1320.1666666666856
40	1433.000000000005
41	1632.4999999999754
42	1772.6666666666179
43	1972.6666666665856
44	2150.999999999892
45	2313.666666666535
46	2520.166666666506
47	2629.4999999998204
48	2908.6666666665856
49	3111.1666666666765
50	3395.666666666823
51	3770.000000000342
52	4169.333333333887
53	4501.500000000735
54	4752.166666667145
55	4960.000000000234
56	4930.166666666866
57	4829.666666666916
58	4787.500000000257
59	4783.000000000231
60	4649.0000000003365
61	4492.166666667125
62	4506.83333333376
63	4486.333333333771
64	4356.500000000552
65	4261.3333333339715
66	3985.1666666673427
67	3659.8333333338637
68	3250.000000000342
69	2845.1666666668248
70	2381.499999999951
71	1801.333333333208
72	1431.4999999999573
73	1063.1666666667074
74	755.0000000000147
75	507.9999999999891
76	343.666666666665
77	179.83333333333385
78	87.99999999999991
79	32.66666666666663
80	12.166666666666668
81	6.666666666666665
82	3.6666666666666665
83	1.8333333333333333
84	1.1666666666666665
85	0.8333333333333333
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08343988177274463
2	0.013283961277252876
3	0.01203858990751042
4	0.011623466117596267
5	0.012453713697424573
6	0.0062268568487122864
7	0.008302475798283047
8	0.0087175995881972
9	0.007057104428540591
10-14	0.007721302492403234
15-19	0.007721302492403234
20-24	0.008219451040300217
25-29	0.009132723378111354
30-34	0.00880062434618003
35-39	0.009049698620128522
40-44	0.00855155007223154
45-49	0.008883649104162861
50-54	0.010710193779785132
55-59	0.008883649104162861
60-64	0.009464822410042675
65-69	0.011706490875579097
70-74	0.019759892399913652
75-79	0.016937950640819134
80-84	0.004069331442063101
85-89	0.0012460024089379906
90-94	0.0018280715341010125
95-99	0.0011638162866113742
100-104	0.003909502503329316
105-109	0.0010831898106834178
110-114	0.0021771769885790323
115-119	0.0032917670374265477
120-124	0.0057007400751817
125-129	0.024487761723731488
130-134	0.01852998350485113
135-139	0.010304056248024695
140-144	0.008312515964793031
145-149	0.0077063950090268726
150-154	0.009438169168358754
155-159	0.011429709451590418
160-164	0.013421331084322325
165-169	0.011516627238815016
170-174	0.008139733241891224
175-179	0.010478249837846919
180-184	0.004763261569745841
185-189	0.005716745647261203
190-194	0.004591579044033243
195-199	0.0032933166471956544
200-204	0.002688675645108692
205-209	0.0
210-214	5.234976272470045E-4
215-219	3.5189583883170583E-4
220-224	9.785545326201155E-4
225-229	7.234509332969196E-4
230-234	4.6443064982207657E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	42.0
80-84	65.0
85-89	55.0
90-94	102.0
95-99	117.0
100-104	209.0
105-109	832.0
110-114	1693.0
115-119	2007.0
120-124	1887.0
125-129	2906.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	5.0
165-169	3.0
170-174	10.0
175-179	14.0
180-184	27.0
185-189	41.0
190-194	66.0
195-199	134.0
200-204	243.0
205-209	610.0
210-214	1659.0
215-219	2167.0
220-224	3162.0
225-229	4698.0
230-234	7361.0
235-239	8240.0
240-244	12222.0
245-249	33914.0
250-253	156401.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.92888752951605
#Duplication Level	Percentage of total
1	70.29612000055225
2	23.25324077016247
3	5.14618017307376
4	0.9591053427597943
5	0.1975539281897463
6	0.03346711157373228
7	0.011867051077682482
8	0.01684056426200095
9	0.0
>10	0.027149408744665267
>50	0.0
>100	0.05847564960389442
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	4.151237899141524E-4	0.0
2	0.0	0.0	0.0	0.0	0.0012453713697424572	0.0
3	0.0	0.0	0.0	0.0	0.0012453713697424572	0.0
4	0.0	0.0	0.0	4.151237899141524E-4	0.002075618949570762	0.0
5	0.0	0.0	0.0	0.002075618949570762	0.0029058665293990667	0.0
6	0.0	0.0	0.0	0.0024907427394849145	0.0037361141092273715	0.0
7	0.0	0.0	0.0	0.0024907427394849145	0.004151237899141524	0.0
8	0.0	0.0	0.0	0.0024907427394849145	0.004566361689055677	0.0
9	0.0	0.0	0.0	0.0033209903193132193	0.004566361689055677	4.151237899141524E-4
10-14	4.1512378991415236E-4	0.0	2.4907427394849145E-4	0.005562658784849642	0.007555252976437573	4.1512378991415236E-4
15-19	4.1512378991415236E-4	0.0	4.1512378991415236E-4	0.0065589558806436075	0.01868057054613686	4.1512378991415236E-4
20-24	4.1512378991415236E-4	0.0	4.1512378991415236E-4	0.011623466117596268	0.026983046344419902	4.1512378991415236E-4
25-29	4.1512378991415236E-4	0.0	5.811733058798134E-4	0.20631652358733374	0.03262872988725238	4.1512378991415236E-4
30-34	4.1512378991415236E-4	0.0	8.302475798283047E-4	1.9108148049748437	0.03951978479982731	8.302475798283047E-4
35-39	4.1512378991415236E-4	0.0	8.302475798283047E-4	5.311342842435614	0.04574664164853959	8.302475798283047E-4
40-44	4.1512378991415236E-4	0.0	8.302475798283047E-4	10.791807116882255	0.04915065672583564	8.302475798283047E-4
45-49	4.1512378991415236E-4	0.0	8.302475798283047E-4	15.980522391777228	0.0523055975291832	9.132723378111353E-4
50-54	4.1512378991415236E-4	0.0	8.302475798283047E-4	20.931371735051393	0.06052504856948342	0.0012453713697424572
55-59	4.1512378991415236E-4	0.0	8.302475798283047E-4	25.35285522142703	0.06982382146356043	0.0012453713697424572
60-64	4.1512378991415236E-4	0.0	8.302475798283047E-4	29.645235209139365	0.09763711538780864	0.0018265446756222706
65-69	4.1512378991415236E-4	0.0	8.302475798283047E-4	33.5715590389054	0.10486026933231489	0.002075618949570762
70-74	4.1512378991415236E-4	0.0	8.302475798283047E-4	37.41137107085333	0.10685286352390282	0.002075618949570762
75-79	4.1512378991415236E-4	0.0	8.302475798283047E-4	41.111452435116156	0.1090115072314564	0.002075618949570762
80-84	4.1512378991415236E-4	0.0	8.302475798283047E-4	44.65976454178636	0.11042292811716455	0.002075618949570762
85-89	4.1512378991415236E-4	0.0	8.302475798283047E-4	47.955681384188765	0.11274762134068379	0.002075618949570762
90-94	4.1512378991415236E-4	0.0	8.302475798283047E-4	51.16375803264533	0.12354083987845174	0.002075618949570762
95-99	4.1512378991415236E-4	0.0	0.0010793218537767965	54.170084519203634	0.266426448366903	0.0022416684655364233
100-104	4.1512378991415236E-4	0.0	0.0012453713697424572	57.02895903558441	0.5440612390614882	0.0024907427394849145
105-109	4.1512378991415236E-4	0.0	0.0014114208857081182	59.70609235674078	1.0096640818292015	0.0027398170134334057
110-114	4.1512378991415236E-4	0.0	0.002075618949570762	62.20264682928449	1.3919930923401358	0.0029058665293990667
115-119	4.1512378991415236E-4	0.0	0.002075618949570762	63.58002756421964	1.6455507032197	0.0029058665293990667
120-124	4.1512378991415236E-4	0.0	0.002075618949570762	63.59945535758764	1.6650615213456654	0.0029058665293990667
125-129	4.1512378991415236E-4	0.0	0.0024077179815020842	63.59995350613552	1.6658917689254935	0.0029058665293990667
130-134	4.1512378991415236E-4	0.0	0.0024907427394849145	63.60427079355063	1.6666389917473388	0.0029058665293990667
135-139	4.1512378991415236E-4	0.0	0.0024907427394849145	63.6102485761254	1.6668050412633046	0.0029058665293990667
140-144	4.1512378991415236E-4	0.0	0.002905866529399067	63.617222655795956	1.6683825116649786	0.0029058665293990667
145-149	4.1512378991415236E-4	0.0	0.003320990319313219	63.63108779037909	1.6687976354548926	0.0029058665293990667
150-154	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	63.6513458313269	1.6687976354548926	0.0029058665293990667
155-159	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	63.682148016538534	1.669544858276738	0.0032379655613303886
160-164	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	63.71751656343922	1.6707902296464805	0.003320990319313219
165-169	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	63.75155671421218	1.6712883781943773	0.003320990319313219
170-174	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	63.790329276190164	1.6712883781943773	0.0034870398352788803
175-179	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	63.838732710094156	1.6717035019842919	0.0037361141092273715
180-184	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	63.89925775866364	1.6721186257742058	0.004151237899141524
185-189	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	63.9694967039171	1.67253374956412	0.004151237899141524
190-194	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	64.02595353934544	1.6736960961758798	0.004151237899141524
195-199	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	64.08448599372332	1.6772661607691415	0.004566361689055677
200-204	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	64.14185610148948	1.6901349982564802	0.004566361689055677
205-209	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	64.20088670441525	1.7131328562177242	0.004566361689055677
210-214	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	64.2500373611411	1.743187818607509	0.00531358451090115
215-219	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	64.30666024608539	1.7807150092157482	0.005811733058798133
220-224	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	64.34875379838267	1.834182953356691	0.005811733058798133
225-229	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	64.39566278664299	1.8808428673430417	0.005811733058798133
230-234	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	64.44688906231839	1.9102336316689636	0.005811733058798133
235-239	4.1512378991415236E-4	4.1512378991415236E-4	0.003320990319313219	64.50749713564585	1.926174385201667	0.005811733058798133
240-241	4.151237899141524E-4	4.151237899141524E-4	0.0033209903193132193	64.59139365358749	1.9274197565714095	0.005811733058798133
>>END_MODULE
