FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR5665096

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5665096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332
Total Bases299.9 kbp
Sequences flagged as poor quality0
Sequence length76-252
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA20.15015015015015015RNA PCR Primer, Index 46 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)