##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665099
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	768736
Total Bases	189.4 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.896374047787535	28.0	18.0	32.0	18.0	33.0
2	28.18980118011905	30.0	27.0	33.0	18.0	33.0
3	30.44167308412771	31.0	29.0	33.0	27.0	33.0
4	29.91976959580402	31.0	29.0	33.0	25.0	33.0
5	31.645510292219956	33.0	32.0	33.0	29.0	33.0
6	34.38119588519336	37.0	34.0	38.0	28.0	38.0
7	35.0592401032344	37.0	35.0	38.0	29.0	38.0
8	35.615584543978684	38.0	36.0	38.0	31.0	38.0
9	36.26116768305374	38.0	37.0	38.0	33.0	38.0
10-14	36.51379381218	38.0	37.6	38.0	33.6	38.0
15-19	36.47097000790909	38.0	38.0	38.0	33.4	38.0
20-24	36.49501727511135	38.0	38.0	38.0	33.6	38.0
25-29	36.45411532697831	38.0	38.0	38.0	33.2	38.0
30-34	36.41951307080714	38.0	38.0	38.0	33.0	38.0
35-39	36.35635224576448	38.0	38.0	38.0	33.0	38.0
40-44	36.37498933313908	38.0	38.0	38.0	33.0	38.0
45-49	36.314541533114095	38.0	37.2	38.0	33.0	38.0
50-54	36.16847110061191	38.0	37.0	38.0	33.0	38.0
55-59	35.99967739249885	38.0	37.0	38.0	31.8	38.0
60-64	35.92305030595679	38.0	36.8	38.0	31.2	38.0
65-69	35.82741253174042	38.0	36.0	38.0	31.0	38.0
70-74	35.660397587728426	38.0	36.0	38.0	30.2	38.0
75-79	35.49519984130764	38.0	36.0	38.0	29.0	38.0
80-84	35.26813625213307	38.0	35.2	38.0	28.4	38.0
85-89	35.071806420815996	38.0	35.0	38.0	27.4	38.0
90-94	34.895071753419906	38.0	35.0	38.0	26.6	38.0
95-99	34.7008635301748	38.0	35.0	38.0	25.4	38.0
100-104	34.47964640548297	38.0	34.2	38.0	23.6	38.0
105-109	34.19862080368169	38.0	34.0	38.0	23.0	38.0
110-114	34.045367181297465	38.0	34.0	38.0	23.0	38.0
115-119	33.919073405894224	38.0	33.4	38.0	23.0	38.0
120-124	34.25756506294662	38.0	33.4	38.0	24.8	38.0
125-129	33.76381108980223	34.2	33.2	35.6	31.0	38.0
130-134	35.45745085157451	36.4	36.0	37.2	33.0	38.0
135-139	36.48943482255265	38.0	38.0	38.0	34.0	38.0
140-144	36.40949936789537	38.0	38.0	38.0	33.0	38.0
145-149	36.389657302444405	38.0	38.0	38.0	33.0	38.0
150-154	36.37889974532595	38.0	38.0	38.0	33.0	38.0
155-159	36.37469419121386	38.0	38.0	38.0	33.0	38.0
160-164	36.34705739855595	38.0	38.0	38.0	33.0	38.0
165-169	36.2999352755112	38.0	38.0	38.0	33.0	38.0
170-174	36.24051128651356	38.0	37.8	38.0	33.0	38.0
175-179	36.16281536731466	38.0	37.0	38.0	33.0	38.0
180-184	36.0121947599313	38.0	37.0	38.0	32.0	38.0
185-189	35.86432953511634	38.0	37.0	38.0	31.0	38.0
190-194	35.70790147866199	38.0	36.6	38.0	30.8	38.0
195-199	35.521686486705946	38.0	36.0	38.0	29.0	38.0
200-204	35.30058523702716	38.0	36.0	38.0	28.2	38.0
205-209	35.063060207920216	38.0	35.0	38.0	27.2	38.0
210-214	34.78478539422203	38.0	35.0	38.0	25.2	38.0
215-219	34.421793252025985	38.0	34.0	38.0	23.2	38.0
220-224	34.13205332722089	38.0	34.0	38.0	23.0	38.0
225-229	33.8039967583068	38.0	34.0	38.0	21.4	38.0
230-234	33.56301236417944	38.0	33.2	38.0	21.0	38.0
235-239	33.26085034799349	38.0	32.8	38.0	21.0	38.0
240-244	33.058930313703165	38.0	31.8	38.0	21.0	38.0
245-249	33.349289834648275	38.0	32.0	38.0	21.8	38.0
250-252	35.05925653495739	37.333333333333336	33.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	0.0
6	0.0
7	2.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.0
17	16.0
18	34.0
19	47.0
20	135.0
21	312.0
22	783.0
23	1526.0
24	2760.0
25	4360.0
26	6340.0
27	8705.0
28	11792.0
29	15175.0
30	19424.0
31	25048.0
32	32518.0
33	44000.0
34	63560.0
35	101975.0
36	200972.0
37	229244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.04615238410157	11.321081401963937	6.577092264411554	34.05567394952295
2	25.59413468830775	13.483790650208203	26.66643251210124	34.25564214938281
3	26.97860350398843	18.07579833594372	20.536499445834917	34.40909871423294
4	18.948413214625795	15.679359562079497	23.73608733433586	41.636139888958844
5	25.02227708926576	26.982921115038238	24.75794397751087	23.23685781818513
6	39.786219106553524	25.35347215085641	15.190099007325058	19.670209735265
7	34.94581972864334	21.223316378962707	16.963693364380212	26.86717052801374
8	31.921522409067432	23.316669463491035	15.326597539575065	29.435210587866468
9	27.34805803330424	13.34029719108505	14.646215646305405	44.66542912930531
10-14	32.44687535204177	16.28449944310732	19.142616820630604	32.126008384220306
15-19	32.594253435468815	18.57745743437298	17.006295833676973	31.82199329648124
20-24	32.19792404899944	17.824112376926433	17.80792978315324	32.17003379092089
25-29	32.304969308824795	17.79703107466855	17.677638201348575	32.22036141515808
30-34	31.87590522845124	17.6817149771714	18.270401283365807	32.17197851101156
35-39	31.12243544013725	17.46049710144701	19.287045983991224	32.13002147442452
40-44	30.083090270162405	17.405222274342805	20.41428839402953	32.09739906146526
45-49	28.32154150434148	17.75112120806885	21.91487612465534	32.012461162934336
50-54	26.932939466677354	18.586159545135004	22.141210250176787	32.33969073801086
55-59	25.89198788845683	18.65448747868538	22.177507267914557	33.276017364943236
60-64	25.52532809102305	19.146825448447004	21.433953290640698	33.89389316988925
65-69	24.535183864536286	19.49674112900477	21.31669263685866	34.651382369600285
70-74	23.60992975525834	19.97433440264649	21.222227801951306	35.19350804014387
75-79	22.305535838067264	19.87197567844928	22.18987628981863	35.632612193664826
80-84	21.523710553744944	20.03195613044096	22.685053887267348	35.75927942854675
85-89	21.074103302861673	19.49228815352035	23.791495879911835	35.64211266370614
90-94	20.983264785063053	18.81516010527099	24.567125869820565	35.634449239845395
95-99	20.301475630177748	18.865374281279234	25.781488069137716	35.0516620194053
100-104	19.996470912485137	20.07631781879489	25.996518279293962	33.93069298942601
105-109	19.31384344818215	22.641489787620195	25.54602169873649	32.49864506546117
110-114	19.054490105434894	24.91113917661237	24.836232243788206	31.198138474164534
115-119	19.274980154566272	25.603959441017025	24.37178285641291	30.74927754800379
120-124	20.19508234064366	25.773001119654275	23.575806804711263	30.4561097349908
125-129	29.03602412071659	19.198398439666295	19.62400582613873	32.14157161347838
130-134	30.92714415223593	20.905816441369215	18.813438944813665	29.35360046158119
135-139	32.208056280779864	16.200225428856672	18.791845820483033	32.799872469880434
140-144	31.61585400967671	17.858088928955464	17.848194438773	32.67786262259483
145-149	31.451069122860652	17.807266810598463	18.526288842214594	32.21537522432629
150-154	31.070032636385434	17.702097963602633	19.06197353280234	32.16589586720959
155-159	30.535169620634882	17.69320857804308	19.527366830603437	32.24425497071859
160-164	29.959270731705402	18.113490657189086	19.92446276972113	32.00277584138438
165-169	29.640849839417594	18.182367897445054	19.951195723107716	32.22558654002964
170-174	29.323837715188	18.480907022450438	20.407088940009654	31.788166322351906
175-179	29.244914301840037	18.23492654536925	20.576956353402128	31.94320279938858
180-184	28.969716928504248	18.368456034821044	21.07841148233673	31.583415554337986
185-189	28.127027413377903	18.101217901088145	22.831286218537294	30.940468466996656
190-194	27.54051369503387	19.17014062834297	22.78825458336421	30.501091093258946
195-199	27.027461044067714	19.40542724078148	23.639037613870883	29.928074101279922
200-204	27.283270847637407	19.381025897941864	23.299286690455013	30.036416563965716
205-209	27.485524302262444	19.21615396491803	23.648322205452505	29.649999527367026
210-214	27.788112127844727	20.39460984678674	22.515255399382927	29.3020226259856
215-219	27.807658664851658	21.20503133283527	22.81548855028807	28.171821452025004
220-224	27.960164543367416	21.58795428108004	24.025961454172716	26.425919721379827
225-229	27.225384939617825	22.95157254053173	23.59397510313972	26.229067416710727
230-234	27.694665192483015	23.094588109882345	23.82035087482567	25.39039582280897
235-239	28.232597213800965	22.88677687761617	23.78183783941734	25.09878806916553
240-244	27.643232585261174	22.565313061627386	24.415430438769206	25.376023914342234
245-249	26.95279806214767	24.40849823597801	23.970260466184158	24.668443235690166
250-252	26.53620078075745	26.012178069467577	21.822452735607797	25.629168414167175
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.0
6	0.0
7	1.0
8	1.0
9	0.0
10	0.5
11	1.0
12	0.5
13	0.5
14	0.8333333333333333
15	0.0
16	0.0
17	0.3333333333333333
18	0.3333333333333333
19	0.8333333333333333
20	3.0
21	4.5
22	9.499999999999996
23	15.500000000000007
24	21.499999999999993
25	27.99999999999998
26	43.99999999999997
27	63.83333333333344
28	87.1666666666666
29	116.49999999999955
30	162.66666666666669
31	207.3333333333346
32	282.66666666666885
33	364.83333333333087
34	409.8333333333287
35	501.16666666665725
36	587.666666666661
37	667.5000000000028
38	822.1666666666853
39	950.8333333333655
40	1112.6666666667074
41	1218.000000000021
42	1377.499999999989
43	1597.9999999999425
44	1841.9999999998893
45	2167.499999999821
46	2661.1666666666824
47	3292.500000000297
48	4319.000000000726
49	5596.166666666236
50	7238.499999998067
51	9301.166666665355
52	11626.333333336264
53	13987.333333340566
54	16542.500000011823
55	18178.00000000604
56	19390.16666666818
57	19786.00000000008
58	19897.833333332987
59	19633.833333333852
60	19420.166666667934
61	19076.33333333586
62	18715.50000000385
63	18135.33333333926
64	17261.666666675723
65	16468.833333345225
66	15422.666666676665
67	14137.00000000767
68	12809.666666671943
69	11236.666666669094
70	9624.166666666166
71	7774.16666666412
72	6019.999999999039
73	4520.500000000392
74	3299.000000000371
75	2255.6666666665656
76	1448.4999999999534
77	860.0000000000266
78	482.1666666666571
79	258.8333333333362
80	134.83333333333272
81	56.00000000000011
82	18.66666666666667
83	5.833333333333332
84	3.0
85	0.6666666666666666
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07609894684260916
2	0.0016910877076135372
3	0.0015610040377971109
4	0.0015610040377971109
5	0.0011707530283478335
6	6.504183490821296E-4
7	7.805020188985554E-4
8	9.105856887149815E-4
9	6.504183490821296E-4
10-14	8.065187528618407E-4
15-19	7.024518170087E-4
20-24	0.0011447362943845481
25-29	0.0017431211755401077
30-34	9.626191566415519E-4
35-39	9.88635890604837E-4
40-44	7.544852849352703E-4
45-49	0.0015089705698705406
50-54	0.002029305249136244
55-59	0.0019252383132831038
60-64	0.002133372184989385
65-69	0.004448861507721767
70-74	0.01048474378720393
75-79	0.011941820698375367
80-84	0.002185520510329446
85-89	6.244687137271493E-4
90-94	9.887922994937385E-4
95-99	4.944332025947854E-4
100-104	0.001769717581701745
105-109	6.507808459057685E-4
110-114	0.0010678551634183033
115-119	0.0021897930098157474
120-124	0.0036268072719834094
125-129	0.024609587591955775
130-134	0.017196386926558078
135-139	0.007747535053670002
140-144	0.006857615486694394
145-149	0.0063341333884734475
150-154	0.007695186843847908
155-159	0.010181750795236616
160-164	0.011542858818011257
165-169	0.009344292417198315
170-174	0.00793100081639576
175-179	0.009684984112699711
180-184	0.004554779468834183
185-189	0.005366875695566725
190-194	0.004896782630509732
195-199	0.0035102462516333126
200-204	0.00243802309358391
205-209	2.3631592919029498E-4
210-214	7.64741916743866E-4
215-219	6.912584519639185E-4
220-224	8.604625524008249E-4
225-229	6.017530159450874E-4
230-234	3.092290245735872E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	23.0
80-84	45.0
85-89	36.0
90-94	54.0
95-99	54.0
100-104	113.0
105-109	302.0
110-114	629.0
115-119	692.0
120-124	791.0
125-129	1883.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	4.0
160-164	6.0
165-169	7.0
170-174	20.0
175-179	24.0
180-184	62.0
185-189	141.0
190-194	241.0
195-199	402.0
200-204	869.0
205-209	2037.0
210-214	5269.0
215-219	7324.0
220-224	10590.0
225-229	16272.0
230-234	25037.0
235-239	29791.0
240-244	45278.0
245-249	125521.0
250-253	495219.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.17755121446237
#Duplication Level	Percentage of total
1	74.3057894505623
2	20.43234891187298
3	4.233018608940259
4	0.817902604893778
5	0.1672087585320727
6	0.02309001745288539
7	0.014429510392040566
8	0.00459141842063176
9	0.0016207189330425637
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3008366981642592E-4	0.0	0.0
5	0.0	0.0	0.0	3.902510094492778E-4	0.0	0.0
6	0.0	0.0	0.0	3.902510094492778E-4	1.3008366981642592E-4	0.0
7	0.0	0.0	1.3008366981642592E-4	3.902510094492778E-4	1.3008366981642592E-4	0.0
8	0.0	0.0	1.3008366981642592E-4	3.902510094492778E-4	2.6016733963285185E-4	0.0
9	0.0	0.0	1.3008366981642592E-4	5.203346792657037E-4	3.902510094492778E-4	0.0
10-14	0.0	0.0	1.3008366981642592E-4	8.065187528618408E-4	7.024518170087E-4	0.0
15-19	0.0	0.0	1.3008366981642592E-4	0.001717104441576822	0.0013268534321275445	0.0
20-24	0.0	0.0	1.3008366981642592E-4	0.008559505473920825	0.002081338717062815	0.0
25-29	0.0	0.0	2.0813387170628148E-4	0.19816946259834328	0.002367522790658952	0.0
30-34	0.0	0.0	2.6016733963285185E-4	1.6278930608167173	0.0037464096907130666	0.0
35-39	0.0	0.0	2.6016733963285185E-4	4.482605211672147	0.004552928443574906	0.0
40-44	0.0	0.0	2.6016733963285185E-4	9.518222120467886	0.00486512925113433	0.0
45-49	0.0	0.0	2.6016733963285185E-4	14.945859176622402	0.005385463930400034	2.0813387170628148E-4
50-54	0.0	7.805020188985555E-5	2.6016733963285185E-4	20.73596137035341	0.005853765141739167	2.6016733963285185E-4
55-59	0.0	1.561004037797111E-4	2.6016733963285185E-4	26.36822003912917	0.006660283894601007	2.6016733963285185E-4
60-64	0.0	4.943179453024186E-4	3.3821754152270746E-4	32.112324647213086	0.0077269699870957	2.6016733963285185E-4
65-69	0.0	6.504183490821297E-4	6.764350830454149E-4	37.57021916496691	0.008013154060691837	2.6016733963285185E-4
70-74	0.0	6.504183490821297E-4	7.805020188985555E-4	42.86139845148399	0.008195271198434833	2.6016733963285185E-4
75-79	0.0	6.504183490821297E-4	7.805020188985555E-4	47.800363193606124	0.008767639345627107	2.6016733963285185E-4
80-84	0.0	6.504183490821297E-4	7.805020188985555E-4	52.47775569246139	0.00962619156641552	2.6016733963285185E-4
85-89	5.203346792657037E-5	7.024518170087E-4	7.805020188985555E-4	56.771271281688385	0.010640844190983642	2.6016733963285185E-4
90-94	1.3008366981642592E-4	8.845689547516963E-4	9.366024226782667E-4	60.761743953711026	0.019850768013986598	2.6016733963285185E-4
95-99	1.3008366981642592E-4	9.105856887149814E-4	0.0010406693585314074	64.43611330807975	0.13411626358073514	2.6016733963285185E-4
100-104	1.3008366981642592E-4	9.105856887149814E-4	0.0010406693585314074	67.77286350580694	0.3558568871498148	2.6016733963285185E-4
105-109	1.3008366981642592E-4	9.105856887149814E-4	0.001144736294384548	70.80089913832578	0.7149658660450402	2.6016733963285185E-4
110-114	1.3008366981642592E-4	9.105856887149814E-4	0.0011707530283478333	73.51673396328518	1.0286236107064064	2.6016733963285185E-4
115-119	1.3008366981642592E-4	9.105856887149814E-4	0.0011707530283478333	74.93391749573325	1.2354306289805603	2.6016733963285185E-4
120-124	1.3008366981642592E-4	9.105856887149814E-4	0.0011707530283478333	74.94684781251301	1.2496617824584773	2.6016733963285185E-4
125-129	1.3008366981642592E-4	9.105856887149814E-4	0.0011707530283478333	74.94820068267909	1.2497138159264038	2.6016733963285185E-4
130-134	1.3008366981642592E-4	9.366024226782667E-4	0.001248803230237689	74.95322191233402	1.2497138159264038	2.6016733963285185E-4
135-139	1.3008366981642592E-4	0.0010666860924946925	0.0013008366981642594	74.96331640511175	1.2498438995962204	2.6016733963285185E-4
140-144	1.3008366981642592E-4	0.0011707530283478333	0.0013008366981642594	74.9746336843858	1.2502081338717062	2.6016733963285185E-4
145-149	1.3008366981642592E-4	0.0013008366981642594	0.0013008366981642594	74.99331369937144	1.2502341506056696	3.902510094492778E-4
150-154	1.3008366981642592E-4	0.0014049036340174	0.001378886900054115	75.01501165549682	1.2502601673396327	3.902510094492778E-4
155-159	1.3008366981642592E-4	0.0014309203679806852	0.0014309203679806852	75.04196499188278	1.2503902510094493	3.902510094492778E-4
160-164	1.3008366981642592E-4	0.0014829538359072557	0.0014309203679806852	75.07456395953878	1.2505723681471923	5.203346792657037E-4
165-169	1.3008366981642592E-4	0.001691087707613537	0.0016650709736502518	75.1192607084877	1.250624401615119	6.504183490821297E-4
170-174	1.3008366981642592E-4	0.001691087707613537	0.0017431211755401074	75.17194459476335	1.250624401615119	7.284685509719852E-4
175-179	1.3008366981642592E-4	0.001691087707613537	0.0019512550472463891	75.23911980185656	1.250624401615119	7.805020188985555E-4
180-184	1.3008366981642592E-4	0.001691087707613537	0.0019512550472463891	75.33462723223577	1.250624401615119	9.105856887149814E-4
185-189	1.3008366981642592E-4	0.001691087707613537	0.002081338717062815	75.42714273820923	1.2511187195604214	9.105856887149814E-4
190-194	1.3008366981642592E-4	0.0019252383132831036	0.002081338717062815	75.51406464638055	1.252289472588769	9.105856887149814E-4
195-199	1.3008366981642592E-4	0.0019512550472463891	0.002081338717062815	75.59614744203472	1.255099279856804	9.105856887149814E-4
200-204	1.3008366981642592E-4	0.0019512550472463891	0.002081338717062815	75.67768388627564	1.2678214627648505	9.366024226782667E-4
205-209	1.3008366981642592E-4	0.00205532198309953	0.002081338717062815	75.75750322607502	1.2948268326187402	0.0010406693585314074
210-214	1.3008366981642592E-4	0.002081338717062815	0.002081338717062815	75.82969966282312	1.3236273571160972	0.0010406693585314074
215-219	1.3008366981642592E-4	0.002081338717062815	0.002081338717062815	75.8968228364484	1.3566686092494693	0.001118719560421263
220-224	1.3008366981642592E-4	0.002081338717062815	0.002081338717062815	75.95221246305624	1.4015734920700995	0.0013008366981642594
225-229	1.3008366981642592E-4	0.0023154893227323816	0.002081338717062815	76.01288348665862	1.4424457811264206	0.0013008366981642594
230-234	1.3008366981642592E-4	0.0024715897265120924	0.0021073554510261	76.07175935561754	1.470699954210548	0.0013008366981642594
235-239	1.3008366981642592E-4	0.0024715897265120924	0.0022114223868792407	76.13828414436166	1.487272613745161	0.0013008366981642594
240-241	1.3008366981642592E-4	0.0024715897265120924	0.0022114223868792407	76.22818496857178	1.4884173500395455	0.0013008366981642594
>>END_MODULE
