##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665100
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	206630
Total Bases	50.3 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.49340850796109	30.0	18.0	32.0	18.0	33.0
2	28.861293132652566	31.0	27.0	33.0	18.0	33.0
3	30.74796012195712	33.0	30.0	33.0	27.0	33.0
4	30.291240381357984	33.0	30.0	33.0	25.0	33.0
5	31.744354643565792	33.0	32.0	33.0	29.0	34.0
6	34.69424575327881	37.0	34.0	38.0	28.0	38.0
7	35.32276048976431	38.0	35.0	38.0	29.0	38.0
8	35.8023955863137	38.0	36.0	38.0	31.0	38.0
9	36.36108503121522	38.0	37.0	38.0	33.0	38.0
10-14	36.569532981658035	38.0	38.0	38.0	34.0	38.0
15-19	36.51874655180758	38.0	38.0	38.0	34.0	38.0
20-24	36.54055461452838	38.0	38.0	38.0	33.8	38.0
25-29	36.48840729806902	38.0	38.0	38.0	34.0	38.0
30-34	36.4483666456952	38.0	38.0	38.0	33.6	38.0
35-39	36.399012728064655	38.0	38.0	38.0	33.4	38.0
40-44	36.42572908096598	38.0	38.0	38.0	33.6	38.0
45-49	36.37880946619561	38.0	38.0	38.0	33.0	38.0
50-54	36.26190582200068	38.0	37.6	38.0	33.0	38.0
55-59	36.12202584329477	38.0	37.0	38.0	32.6	38.0
60-64	36.068428592169575	38.0	37.0	38.0	32.4	38.0
65-69	35.97387891400088	38.0	37.0	38.0	31.8	38.0
70-74	35.7913013599187	38.0	36.4	38.0	31.2	38.0
75-79	35.61042596690699	38.0	36.0	38.0	30.4	38.0
80-84	35.348209923629426	38.0	36.0	38.0	28.4	38.0
85-89	35.19437199170219	38.0	35.0	38.0	28.0	38.0
90-94	35.056834418671215	38.0	35.0	38.0	27.0	38.0
95-99	34.90336997194383	38.0	35.0	38.0	26.6	38.0
100-104	34.72633237592814	38.0	35.0	38.0	25.0	38.0
105-109	34.46715269455027	38.0	34.0	38.0	23.8	38.0
110-114	34.36374357537203	38.0	34.0	38.0	23.4	38.0
115-119	34.255106424373636	38.0	34.0	38.0	23.6	38.0
120-124	34.57619592941154	38.0	34.0	38.0	26.4	38.0
125-129	33.657974167108804	34.2	33.2	35.6	31.0	38.0
130-134	35.14625707935339	36.2	36.0	37.2	32.2	38.0
135-139	36.207324240601054	38.0	38.0	38.0	33.0	38.0
140-144	36.13712788332927	38.0	38.0	38.0	33.0	38.0
145-149	36.12429032434502	38.0	37.8	38.0	33.0	38.0
150-154	36.10929378011366	38.0	37.4	38.0	33.0	38.0
155-159	36.10372410897847	38.0	37.0	38.0	33.0	38.0
160-164	36.08193046660567	38.0	37.0	38.0	32.8	38.0
165-169	36.03286460146189	38.0	37.0	38.0	32.2	38.0
170-174	35.99008897920491	38.0	37.0	38.0	31.8	38.0
175-179	35.90512337985164	38.0	37.0	38.0	31.0	38.0
180-184	35.75986836132046	38.0	37.0	38.0	30.6	38.0
185-189	35.603885141096214	38.0	36.2	38.0	29.0	38.0
190-194	35.4488834906052	38.0	36.0	38.0	29.0	38.0
195-199	35.27694060794149	38.0	36.0	38.0	27.8	38.0
200-204	35.0744029279975	38.0	35.0	38.0	27.0	38.0
205-209	34.838837442409115	38.0	35.0	38.0	25.8	38.0
210-214	34.60922461563509	38.0	34.8	38.0	24.0	38.0
215-219	34.303410295779045	38.0	34.0	38.0	23.0	38.0
220-224	34.08056191750025	38.0	34.0	38.0	23.0	38.0
225-229	33.82701453576025	38.0	34.0	38.0	21.4	38.0
230-234	33.64619290106489	38.0	33.4	38.0	21.0	38.0
235-239	33.43505114075188	38.0	33.0	38.0	21.0	38.0
240-244	33.314484071776974	38.0	32.6	38.0	21.0	38.0
245-249	33.65395404967952	38.0	32.8	38.0	22.6	38.0
250-252	35.22308232425718	37.333333333333336	33.333333333333336	38.0	30.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	2.0
5	4.0
6	1.0
7	3.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	5.0
17	19.0
18	42.0
19	49.0
20	107.0
21	202.0
22	376.0
23	634.0
24	990.0
25	1452.0
26	1968.0
27	2643.0
28	3338.0
29	4160.0
30	5399.0
31	6664.0
32	8484.0
33	11204.0
34	15636.0
35	24494.0
36	48045.0
37	70704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27155949082596	15.209830856567145	9.776122294770698	31.742487357836204
2	21.151556222469626	15.887022605159979	33.04419381383417	29.91722735853623
3	24.317626273627145	19.215857111740362	24.656937486386408	31.809579128246085
4	17.87691920463126	14.5593331913493	27.953106546109314	39.61064105791012
5	21.851660027203245	27.930278373420204	28.252651348303186	21.965410251073365
6	37.88223450492222	28.553244213848046	14.730850765195003	18.833670516034733
7	32.67974172563673	24.61544418737476	18.032739276483287	24.672074810505222
8	29.515830029864325	29.001311707106936	17.067197157806593	24.415661105222146
9	26.402373508087546	13.274027897431925	17.71709564695519	42.606502947525335
10-14	30.061082446782766	17.664540236004765	21.771197351480595	30.503179965731874
15-19	30.296899548227906	21.09495926113434	19.341305250604282	29.266835940033474
20-24	29.644472322894405	20.39995895672645	20.189612179785026	29.765956540594118
25-29	29.80059495287133	20.251146392674706	20.089291332332387	29.858967322121575
30-34	29.56697166709906	19.942226748395942	20.683172572419856	29.80762901208514
35-39	28.846881749159486	19.577947316502726	21.60766854566171	29.967502388676078
40-44	27.785339107909323	19.423658853435608	22.72451826367767	30.0664837749774
45-49	26.309731703444996	19.722158763102158	23.91388102641147	30.05422850704138
50-54	25.349902517129685	20.553396798844535	23.42831862861834	30.668382055407445
55-59	24.78449631913421	20.359906877241553	23.149814383690934	31.705782419933303
60-64	24.959996437674302	20.749385547218722	21.9563169811613	32.33430103394567
65-69	25.05188670112873	21.251185843449306	21.34247158815899	32.35445586726298
70-74	25.187684235492284	21.992865336863556	21.215796938000068	31.60365348964409
75-79	25.260032450810687	21.969353733198574	21.598667115866434	31.171946700124302
80-84	24.941962116122237	21.82059855984727	22.413756382231018	30.823682941799476
85-89	24.662031971649608	21.07621103999845	23.510103699687253	30.751653288664688
90-94	24.590027397684803	20.163785664686813	24.130203405293013	31.115983532335367
95-99	23.88569214375666	20.25641770803061	25.183958151700086	30.673931996512643
100-104	23.439068635241313	21.80245516246943	25.17297383201479	29.58550237027447
105-109	22.556505506958406	24.877558465347686	24.27505297391277	28.290883053781137
110-114	22.061539510001023	27.077968543876473	23.412079112491423	27.448412833631085
115-119	22.190045993080478	27.179482661058994	23.262532575984523	27.367938769875998
120-124	22.75500544187429	26.8905346813699	22.998659704741417	27.355800172014398
125-129	27.47919986189462	20.849546494484713	22.208475622218298	29.462778021402364
130-134	28.907724839517567	23.29059106470421	20.620227052553894	27.181457043224327
135-139	30.31353028637921	17.47872917049981	21.07778833100444	31.129952212116542
140-144	29.3360517155644	19.992739930382893	19.993237195425163	30.67797115862755
145-149	29.196705708719172	20.144963137632434	20.67425362780794	29.98407752584045
150-154	29.036140024943357	19.81763897370206	21.015278305442784	30.130942695911795
155-159	28.536220280572284	19.686109135755807	21.51843213701396	30.25923844665795
160-164	28.08986640781025	20.013367175627184	21.742447147938034	30.154319268624537
165-169	27.9522398154042	20.052912157861236	21.655991406747294	30.33885661998727
170-174	27.790823151477177	20.28285420178723	21.880721855062085	30.045600791673504
175-179	27.86366746765544	19.97555260268305	21.86241824344754	30.298361686213976
180-184	27.838559331305664	20.13850900417934	22.05790205505748	29.96502960945752
185-189	27.39875504061337	19.708872872100216	23.390534886913205	29.50183720037321
190-194	27.206583186251006	20.378248662330144	23.2590393451039	29.156128806314957
195-199	26.793733577514132	20.42111234970937	23.999024603869735	28.78612946890676
200-204	27.020927144930262	20.36549545969301	23.67622934899856	28.937348046378165
205-209	27.279038728945267	20.12895683417356	23.969033601793903	28.62297083508727
210-214	27.431006725260147	21.13823500240939	23.115639811376138	28.31511846095432
215-219	27.20207986840164	21.74677016160812	23.16864677198038	27.882503198009857
220-224	27.373488511770148	22.211701303980995	24.196579782268067	26.21823040198079
225-229	26.64148687402112	23.39925124886997	23.780892211597443	26.178369665511468
230-234	27.103387950162144	23.71042413381123	23.673088410991635	25.513099505034987
235-239	27.510711557410627	23.328388118198024	23.766925367846508	25.393974956544845
240-244	27.035935681708224	22.92676305051276	24.380410279138786	25.656890988640225
245-249	26.43761251446728	24.240999795457117	24.108364533534235	25.213023156541368
250-252	26.132753391545865	25.465580149221466	22.222404181869397	26.179262277363275
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.5
3	1.5
4	3.0
5	2.5
6	1.0
7	0.5
8	1.0
9	2.0
10	1.0
11	1.0
12	1.5
13	1.0
14	1.3333333333333333
15	2.333333333333333
16	2.833333333333333
17	3.0
18	6.0
19	8.833333333333334
20	12.999999999999996
21	15.833333333333337
22	21.500000000000007
23	23.83333333333334
24	45.66666666666666
25	62.66666666666678
26	84.00000000000006
27	110.16666666666639
28	149.9999999999995
29	184.50000000000048
30	241.3333333333353
31	297.5000000000016
32	364.66666666666447
33	437.49999999999426
34	511.16666666665725
35	592.9999999999924
36	672.8333333333343
37	776.6666666666781
38	920.333333333358
39	993.5000000000315
40	1067.666666666706
41	1211.1666666667018
42	1330.33333333335
43	1437.5000000000002
44	1533.3333333333185
45	1653.1666666666379
46	1803.4999999999434
47	1979.8333333332396
48	2200.4999999998627
49	2494.666666666517
50	2774.999999999986
51	3137.500000000166
52	3595.8333333337214
53	3924.8333333338874
54	4107.3333333339815
55	4243.8333333340615
56	4360.833333333963
57	4240.166666667387
58	4212.166666667382
59	4129.000000000727
60	4042.000000000694
61	4018.666666667354
62	3965.333333333999
63	3994.5000000006826
64	3823.5000000006085
65	3643.3333333338614
66	3543.500000000483
67	3210.500000000331
68	2871.0000000001783
69	2479.500000000002
70	2062.3333333331475
71	1692.1666666665637
72	1373.6666666666354
73	1044.5000000000448
74	770.3333333333497
75	542.1666666666578
76	362.99999999999716
77	215.50000000000165
78	117.6666666666661
79	66.66666666666681
80	36.833333333333314
81	13.500000000000005
82	4.833333333333333
83	0.6666666666666666
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08614431592701931
2	0.01935827324202681
3	0.01693848908677346
4	0.01645453225572279
5	0.0179064027488748
6	0.007743309296810724
7	0.013550791269418768
8	0.014034748100469438
9	0.007743309296810724
10-14	0.011614963945216087
15-19	0.010356676184484345
20-24	0.009098388423752601
25-29	0.012292503508687025
30-34	0.014131539466679574
35-39	0.014615496297730244
40-44	0.01122779848037555
45-49	0.011034215747955283
50-54	0.01432512219909984
55-59	0.010163093452064077
60-64	0.011324589846585685
65-69	0.012582877607317427
70-74	0.015970575424672118
75-79	0.015196906413191304
80-84	0.0036786629802591326
85-89	0.002614209487256697
90-94	0.002324245854852783
95-99	0.0017436487593939078
100-104	0.0039729105639691896
105-109	0.001067228611040771
110-114	0.0011682732045831356
115-119	0.001664260016153112
120-124	0.0052152675481452835
125-129	0.02261559245908859
130-134	0.015812006185577262
135-139	0.007955726382680384
140-144	0.006464027685927812
145-149	0.006762367425278326
150-154	0.008651852441164917
155-159	0.011635284547347653
160-164	0.011138072317912407
165-169	0.010939286957386505
170-174	0.008353645620452111
175-179	0.009447857275695437
180-184	0.004177175910823261
185-189	0.0038791572878921557
190-194	0.004178489137917998
195-199	0.0032843892976681835
200-204	0.0020915751353547908
205-209	0.0
210-214	5.009106555718296E-4
215-219	3.031279776008633E-4
220-224	4.085776798099297E-4
225-229	2.0758236089646516E-4
230-234	1.0667337997804661E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	24.0
80-84	26.0
85-89	40.0
90-94	55.0
95-99	52.0
100-104	131.0
105-109	497.0
110-114	1039.0
115-119	1137.0
120-124	982.0
125-129	1534.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	1.0
160-164	2.0
165-169	0.0
170-174	4.0
175-179	8.0
180-184	14.0
185-189	33.0
190-194	56.0
195-199	121.0
200-204	223.0
205-209	500.0
210-214	1457.0
215-219	1929.0
220-224	2585.0
225-229	4202.0
230-234	6503.0
235-239	7207.0
240-244	10746.0
245-249	30224.0
250-253	135298.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.75523438594104
#Duplication Level	Percentage of total
1	84.26691104483736
2	13.68285137000591
3	1.7181028591784206
4	0.23517840699588963
5	0.05499022450901357
6	0.011819288133325789
7	0.006844439947997234
8	0.0
9	0.008738387397031082
>10	0.014563978995051803
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	4.839568310506703E-4	0.0
4	0.0	4.839568310506703E-4	0.0	0.0	4.839568310506703E-4	0.0
5	0.0	4.839568310506703E-4	0.0	0.0014518704931520108	4.839568310506703E-4	0.0
6	0.0	4.839568310506703E-4	0.0	0.0014518704931520108	4.839568310506703E-4	0.0
7	0.0	4.839568310506703E-4	0.0	0.0014518704931520108	4.839568310506703E-4	0.0
8	0.0	4.839568310506703E-4	0.0	0.0014518704931520108	9.679136621013406E-4	0.0
9	0.0	4.839568310506703E-4	0.0	0.0014518704931520108	9.679136621013406E-4	0.0
10-14	0.0	4.8395683105067024E-4	0.0	0.0014518704931520108	0.003484489183564826	0.0
15-19	0.0	4.8395683105067024E-4	9.679136621013406E-5	0.001839035957992547	0.01171175531142622	0.0
20-24	0.0	4.8395683105067024E-4	7.743309296810725E-4	0.0047427769442965695	0.018680733678555873	0.0
25-29	0.0	4.8395683105067024E-4	9.679136621013405E-4	0.16870735130426368	0.023229927890432173	0.0
30-34	0.0	4.8395683105067024E-4	9.679136621013405E-4	1.7910274403523208	0.02797270483472874	1.9358273242026813E-4
35-39	0.0	4.8395683105067024E-4	9.679136621013405E-4	4.987271935343367	0.03203794221555437	8.711222958912065E-4
40-44	0.0	4.8395683105067024E-4	9.679136621013405E-4	10.395683105067027	0.03358660407491652	9.679136621013405E-4
45-49	0.0	4.8395683105067024E-4	9.679136621013405E-4	15.537240478149348	0.034748100469438124	9.679136621013405E-4
50-54	0.0	4.8395683105067024E-4	9.679136621013405E-4	20.644533707593283	0.03823258965300295	9.679136621013405E-4
55-59	0.0	4.8395683105067024E-4	9.679136621013405E-4	25.29332623529981	0.043072157963509654	9.679136621013405E-4
60-64	0.0	4.8395683105067024E-4	9.679136621013405E-4	29.94918453273968	0.05110584135895078	9.679136621013405E-4
65-69	0.0	4.8395683105067024E-4	0.0012582877607317427	34.332381551565604	0.05410637371146494	9.679136621013405E-4
70-74	0.0	4.8395683105067024E-4	0.0014518704931520108	38.57571504621787	0.05604220103566763	0.0012582877607317427
75-79	0.0	4.8395683105067024E-4	0.0014518704931520108	42.613754053138464	0.058849150655761504	0.0014518704931520108
80-84	0.0	4.8395683105067024E-4	0.0014518704931520108	46.59429898853023	0.05981706431786284	0.0014518704931520108
85-89	0.0	4.8395683105067024E-4	0.0014518704931520108	50.305279969026756	0.06107535207859459	0.0014518704931520108
90-94	0.0	4.8395683105067024E-4	0.0014518704931520108	53.77689590088564	0.07220635919276001	0.001645453225572279
95-99	0.0	4.8395683105067024E-4	0.0014518704931520108	57.07438416493248	0.22271693364951844	0.001935827324202681
100-104	0.0	4.8395683105067024E-4	0.0014518704931520108	60.09582345254803	0.5264482408169191	0.001935827324202681
105-109	0.0	4.8395683105067024E-4	0.0014518704931520108	62.91187146106567	1.0375066544064269	0.001935827324202681
110-114	0.0	4.8395683105067024E-4	0.0014518704931520108	65.58011905338044	1.475874751972124	0.0024197841552533513
115-119	0.0	4.8395683105067024E-4	0.001645453225572279	67.07109325848134	1.7654745196728452	0.0024197841552533513
120-124	0.0	4.8395683105067024E-4	0.001935827324202681	67.09287131587863	1.7898659439577986	0.0024197841552533513
125-129	0.0	4.8395683105067024E-4	0.001935827324202681	67.09316168997725	1.7901563180564293	0.0024197841552533513
130-134	0.0	4.8395683105067024E-4	0.001935827324202681	67.09867879785124	1.7901563180564293	0.0024197841552533513
135-139	0.0	4.8395683105067024E-4	0.001935827324202681	67.10680927261288	1.79054348352127	0.0024197841552533513
140-144	0.0	9.679136621013405E-4	0.001935827324202681	67.11755311426221	1.7923825194792624	0.0024197841552533513
145-149	0.0	9.679136621013405E-4	0.001935827324202681	67.13129748826404	1.793544015873784	0.0024197841552533513
150-154	0.0	9.679136621013405E-4	0.001935827324202681	67.14765522915357	1.7937375986062043	0.0024197841552533513
155-159	0.0	9.679136621013405E-4	0.001935827324202681	67.16962686928326	1.7945119295358853	0.0024197841552533513
160-164	0.0	9.679136621013405E-4	0.001935827324202681	67.19663166045589	1.7945119295358853	0.0024197841552533513
165-169	0.0	9.679136621013405E-4	0.001935827324202681	67.22876639403765	1.7948023036345155	0.0024197841552533513
170-174	0.0	9.679136621013405E-4	0.001935827324202681	67.26564390456372	1.795673425930407	0.0024197841552533513
175-179	0.0	9.679136621013405E-4	0.001935827324202681	67.3103615157528	1.7959638000290377	0.0024197841552533513
180-184	0.0	9.679136621013405E-4	0.001935827324202681	67.36408072399941	1.7963509654938778	0.0024197841552533513
185-189	0.0	9.679136621013405E-4	0.001935827324202681	67.42351062285243	1.796447756860088	0.0024197841552533513
190-194	0.0	9.679136621013405E-4	0.001935827324202681	67.48003678071916	1.7976092532546097	0.0024197841552533513
195-199	0.0	9.679136621013405E-4	0.001935827324202681	67.53249770120505	1.799157915113972	0.0024197841552533513
200-204	0.0	9.679136621013405E-4	0.0021294100566229494	67.58302279436676	1.8126119150171802	0.0024197841552533513
205-209	0.0	9.679136621013405E-4	0.0024197841552533513	67.63141847747181	1.8416493248802204	0.0024197841552533513
210-214	0.0	9.679136621013405E-4	0.0025165755214634853	67.67933020374582	1.8716546484053622	0.0024197841552533513
215-219	0.0	9.679136621013405E-4	0.0029037409863040217	67.72143444804723	1.903305425156076	0.0024197841552533513
220-224	0.0	9.679136621013405E-4	0.0029037409863040217	67.75850554130571	1.9469583313168468	0.0024197841552533513
225-229	0.0	9.679136621013405E-4	0.0029037409863040217	67.79925470648018	1.991385568407298	0.0024197841552533513
230-234	0.0	9.679136621013405E-4	0.0029037409863040217	67.84029424575326	2.020810143735179	0.0024197841552533513
235-239	0.0	0.0013550791269418769	0.0029037409863040217	67.8940134539999	2.03600638823017	0.0024197841552533513
240-241	0.0	0.0019358273242026812	0.0029037409863040217	67.97657648937715	2.0372162803077964	0.0024197841552533513
>>END_MODULE
