##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665102
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174661
Total Bases	42.3 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.158300937244146	30.0	18.0	32.0	18.0	33.0
2	28.70339114055227	31.0	27.0	33.0	18.0	33.0
3	30.592147073473758	33.0	29.0	33.0	27.0	33.0
4	30.0466847206875	31.0	29.0	33.0	25.0	33.0
5	31.625961147594484	33.0	32.0	33.0	29.0	34.0
6	34.43288427296305	37.0	34.0	38.0	28.0	38.0
7	35.05804386783541	37.0	35.0	38.0	29.0	38.0
8	35.58534532608882	38.0	36.0	38.0	29.0	38.0
9	36.24563010632024	38.0	37.0	38.0	33.0	38.0
10-14	36.48201258437774	38.0	37.8	38.0	33.6	38.0
15-19	36.416909327210995	38.0	38.0	38.0	33.0	38.0
20-24	36.43414843611338	38.0	38.0	38.0	33.2	38.0
25-29	36.38286280280085	38.0	38.0	38.0	33.0	38.0
30-34	36.32316773635786	38.0	38.0	38.0	33.0	38.0
35-39	36.25348532299712	38.0	37.2	38.0	33.0	38.0
40-44	36.26895529053424	38.0	37.0	38.0	33.0	38.0
45-49	36.21904947297909	38.0	37.0	38.0	33.0	38.0
50-54	36.092851867331575	38.0	37.0	38.0	32.6	38.0
55-59	35.92673121074539	38.0	37.0	38.0	31.2	38.0
60-64	35.86474141336647	38.0	36.6	38.0	31.0	38.0
65-69	35.76249305798089	38.0	36.0	38.0	31.0	38.0
70-74	35.544223381292895	38.0	36.0	38.0	29.6	38.0
75-79	35.26848601875296	38.0	35.4	38.0	28.4	38.0
80-84	34.901103572744944	38.0	35.0	38.0	26.4	38.0
85-89	34.70629551413293	38.0	35.0	38.0	25.6	38.0
90-94	34.57516297327427	38.0	34.8	38.0	24.0	38.0
95-99	34.41078285123028	38.0	34.0	38.0	23.2	38.0
100-104	34.215744692018035	38.0	34.0	38.0	23.0	38.0
105-109	33.983924561705365	38.0	34.0	38.0	23.0	38.0
110-114	33.87093785673772	38.0	33.8	38.0	23.0	38.0
115-119	33.784604200011735	38.0	33.0	38.0	23.0	38.0
120-124	34.21249354823575	38.0	33.0	38.0	24.8	38.0
125-129	33.78144063796871	34.2	33.2	35.6	31.0	38.0
130-134	35.324714046733774	36.4	36.0	37.2	32.2	38.0
135-139	36.30953650448363	38.0	38.0	38.0	33.0	38.0
140-144	36.236125577078816	38.0	38.0	38.0	33.0	38.0
145-149	36.2120126972117	38.0	38.0	38.0	33.0	38.0
150-154	36.20356277267615	38.0	38.0	38.0	33.0	38.0
155-159	36.20953740047547	38.0	38.0	38.0	33.0	38.0
160-164	36.17052634067909	38.0	37.2	38.0	33.0	38.0
165-169	36.11827442647596	38.0	37.0	38.0	33.0	38.0
170-174	36.05325240957861	38.0	37.0	38.0	32.4	38.0
175-179	35.95454992208984	38.0	37.0	38.0	31.4	38.0
180-184	35.79574262901379	38.0	37.0	38.0	31.0	38.0
185-189	35.62209173501735	38.0	36.2	38.0	29.0	38.0
190-194	35.4408007857254	38.0	36.0	38.0	29.0	38.0
195-199	35.22865236430658	38.0	35.8	38.0	27.8	38.0
200-204	34.9626401694707	38.0	35.0	38.0	26.8	38.0
205-209	34.705859413065426	38.0	35.0	38.0	24.8	38.0
210-214	34.40662723045661	38.0	34.2	38.0	23.2	38.0
215-219	34.04822457362634	38.0	34.0	38.0	23.0	38.0
220-224	33.78134111910738	38.0	34.0	38.0	21.0	38.0
225-229	33.51138284093504	38.0	33.4	38.0	21.0	38.0
230-234	33.32148748477991	38.0	33.0	38.0	21.0	38.0
235-239	33.09128653467705	38.0	32.4	38.0	21.0	38.0
240-244	32.96439962982349	37.6	31.4	38.0	21.0	38.0
245-249	33.34214347178839	38.0	32.0	38.0	21.8	38.0
250-252	35.089889884369654	37.333333333333336	33.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	2.0
6	1.0
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	15.0
17	19.0
18	34.0
19	56.0
20	75.0
21	165.0
22	291.0
23	568.0
24	889.0
25	1269.0
26	1728.0
27	2276.0
28	2968.0
29	3730.0
30	4830.0
31	5897.0
32	7824.0
33	10258.0
34	14475.0
35	23292.0
36	45176.0
37	48820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.43620672859279	14.944787947899535	9.876281452532535	32.74272387097514
2	20.816536875858908	15.82226294090701	33.62288135593221	29.738318827301878
3	23.635509951673118	19.64396143010925	24.79558415977646	31.924944458441175
4	17.65763496026202	14.997366070406084	28.07482650420284	39.27017246512906
5	21.7180190445652	27.86140870232417	28.434577967625387	21.985994285485248
6	37.40481179932896	28.878239261625843	14.948412289440839	18.76853664960436
7	32.344689378757515	24.740910392212996	18.436301173776123	24.478099055253363
8	28.873912819426394	29.749383048285416	17.13608437494632	24.240619757341868
9	26.635099311205646	13.108850119952134	17.780971411885282	42.47507915695694
10-14	29.881855003555685	17.72174946664697	21.95147088631078	30.444924643486566
15-19	30.155761884258453	21.120954221900565	19.421452586641262	29.30183130719972
20-24	29.574871316267114	20.477976330209042	20.255592517735167	29.691559835788677
25-29	29.677216922092096	20.434377823507386	20.22664784042123	29.661757413979288
30-34	29.427581357745048	19.985296172195106	20.83798076592743	29.749141704132416
35-39	28.485132346508053	19.572441877243087	21.976826478004853	29.965599298244005
40-44	27.104370549691154	19.34294031289865	23.67489871217309	29.877790425237098
45-49	25.127739441313146	19.982204653357183	25.00314911217939	29.88690679315028
50-54	23.927584544437433	20.92611530176733	24.417141240828684	30.729158912966554
55-59	23.300013627145276	20.54973507226395	23.975988741000645	32.17426255959013
60-64	23.98694995957648	21.147569893753708	21.961765992483286	32.903714154186524
65-69	24.476381334096764	21.757801316919554	20.57028342399084	33.195533924992844
70-74	25.382750489854207	21.935505297067518	19.84313213523755	32.83861207784072
75-79	26.151788209316923	21.37628211882304	20.279008296265076	32.19292137559496
80-84	25.657001815380916	21.10194194217124	21.469484993041995	31.77157124940585
85-89	25.47641113350051	20.170391083851797	22.748023044151154	31.60517473849654
90-94	25.405498927530385	18.789644710066547	23.754972775771353	32.04988358663171
95-99	24.440104569590236	18.832153429857346	25.255435635950207	31.47230636460221
100-104	23.84770090560309	20.93780027197905	25.119191492972348	30.095307329445514
105-109	22.631194582044408	25.271621184041003	23.863623315213772	28.23356091870081
110-114	22.122054464296014	27.912548010934383	22.816180117417733	27.149217407351873
115-119	22.342174179528612	27.97705280362549	22.467543322003678	27.213229694842223
120-124	23.127600554785023	27.317441637829987	22.157894245272885	27.397063562112105
125-129	27.39425818932438	21.237838468693305	22.02084480276893	29.34705853921339
130-134	28.752580708479858	23.408237928906065	20.736480398440104	27.10270096417397
135-139	30.10873973286229	17.678689873619767	21.299485327762152	30.91308506575579
140-144	29.192170587087734	20.04752980958518	20.388278344969464	30.372021258357616
145-149	28.913087172938912	20.217311625817285	21.092233296681208	29.777367904562595
150-154	28.545973926471042	19.984463714378276	21.634455083108488	29.835107276042194
155-159	28.025705147532648	19.820395036328918	22.092947637881394	30.06095217825704
160-164	27.328052744404594	20.367985867980547	22.450188533341848	29.853772854273007
165-169	27.173567321437652	20.42339203136658	22.257878002840112	30.14516264435566
170-174	27.083234801691276	20.59504441018171	22.452562926247886	29.86915786187913
175-179	27.247541423627165	20.139338049669334	22.50030447681424	30.11281604988926
180-184	27.37447806359376	20.273186629440822	22.601102821619847	29.751232485345575
185-189	26.87358733590624	20.042177374428874	23.97995451088557	29.10428077877932
190-194	26.859727564538666	20.741820487913383	23.835440746529482	28.56301120101847
195-199	26.63897791569533	20.6152538339409	24.539234478651522	28.206533771712245
200-204	27.045855076105134	20.356212082245452	24.16259464994483	28.435338191704584
205-209	27.326568057526618	20.13752234144256	24.42886069034315	28.10704891068767
210-214	27.523947383180804	21.596500167664498	23.246414306734145	27.63313814242055
215-219	27.331778151503645	22.115047288377728	23.394636431038535	27.15853812908009
220-224	27.21427094889725	23.084732535909165	24.484770943392995	25.21622557180059
225-229	26.263125	24.422375	24.042125	25.272375000000004
230-234	26.823569940154346	24.770608933721686	23.911582290038375	24.494238836085593
235-239	27.150388532711244	24.295393617079124	24.13824042806571	24.415977422143918
240-244	26.496123673014644	24.218673332140323	24.44669256657296	24.838510428272077
245-249	25.962301874298866	25.405894510821174	24.26800770162936	24.3637959132506
250-252	25.1643529190786	27.329969434625955	22.180813824428395	25.324863821867048
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.5
7	1.5
8	2.333333333333333
9	1.0
10	0.3333333333333333
11	0.0
12	0.0
13	0.3333333333333333
14	0.6666666666666666
15	1.3333333333333333
16	2.333333333333333
17	1.6666666666666665
18	4.166666666666666
19	8.5
20	11.166666666666664
21	19.000000000000007
22	20.66666666666667
23	24.83333333333332
24	32.8333333333333
25	46.50000000000003
26	73.66666666666676
27	109.83333333333293
28	113.66666666666623
29	126.49999999999935
30	185.33333333333394
31	232.66666666666848
32	259.5000000000026
33	312.6666666666672
34	379.83333333333024
35	440.16666666666066
36	504.3333333333238
37	601.1666666666605
38	660.1666666666661
39	745.0000000000085
40	822.3333333333499
41	916.0000000000254
42	1049.5000000000373
43	1200.333333333372
44	1278.8333333333642
45	1416.1666666666745
46	1571.1666666666483
47	1742.1666666666163
48	1893.4999999999195
49	2174.4999999998636
50	2483.1666666665137
51	2732.9999999999714
52	3086.166666666803
53	3443.500000000314
54	3766.833333333816
55	3986.8333333339197
56	4125.833333333995
57	4109.333333334007
58	3955.6666666672872
59	3825.500000000566
60	3671.5000000005025
61	3545.8333333337837
62	3321.666666667024
63	3214.5000000003142
64	3112.3333333336036
65	2947.6666666668657
66	2729.5000000001
67	2504.833333333332
68	2194.4999999998627
69	1878.6666666665262
70	1581.1666666665924
71	1286.333333333326
72	1025.8333333333771
73	784.0000000000168
74	564.833333333326
75	414.6666666666612
76	272.83333333333576
77	188.83333333333414
78	130.33333333333263
79	74.66666666666673
80	41.16666666666667
81	24.833333333333318
82	10.333333333333332
83	5.666666666666665
84	2.6666666666666665
85	0.6666666666666666
86	0.6666666666666666
87	0.3333333333333333
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0870257241170038
2	0.012023290831954471
3	0.009733140197296477
4	0.009733140197296477
5	0.010305677855960977
6	0.0040077636106514904
7	0.0062979142453094845
8	0.006870451903973984
9	0.004580301269315989
10-14	0.006412421777042384
15-19	0.005610869054912087
20-24	0.004580301269315989
25-29	0.006412421777042384
30-34	0.007213974499172683
35-39	0.007672004626104282
40-44	0.005839884118377886
45-49	0.004923823864514688
50-54	0.007557497094371382
55-59	0.005496361523179187
60-64	0.005839884118377886
65-69	0.0062979142453094845
70-74	0.009847647729029376
75-79	0.009046737277023691
80-84	0.0028632948506505406
85-89	0.0010309963319441611
90-94	0.0012603650704715943
95-99	4.5843538295972755E-4
100-104	0.002407850970077292
105-109	3.4447716977557315E-4
110-114	0.0024221145983153616
115-119	0.0034823549076827714
120-124	0.005603575080901615
125-129	0.02651757113191136
130-134	0.018561100897908033
135-139	0.008748544372262385
140-144	0.008275650081869824
145-149	0.007684532218879121
150-154	0.010640159271361893
155-159	0.01229533876613911
160-164	0.01194079258488603
165-169	0.01040399418795052
170-174	0.008039535598590717
175-179	0.009813241017928674
180-184	0.0057940304931547665
185-189	0.004849098422505494
190-194	0.0039042904602803515
195-199	0.0030781871372030883
200-204	0.002014733628511562
205-209	2.3751643316821983E-4
210-214	4.773343739222685E-4
215-219	4.8222459851788267E-4
220-224	4.892649161583408E-4
225-229	4.999975000125E-4
230-234	1.2950784429012865E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	28.0
80-84	27.0
85-89	36.0
90-94	43.0
95-99	56.0
100-104	119.0
105-109	540.0
110-114	1060.0
115-119	1080.0
120-124	936.0
125-129	1565.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	2.0
160-164	1.0
165-169	2.0
170-174	2.0
175-179	10.0
180-184	35.0
185-189	42.0
190-194	89.0
195-199	148.0
200-204	242.0
205-209	540.0
210-214	1351.0
215-219	2116.0
220-224	2933.0
225-229	4570.0
230-234	6896.0
235-239	7760.0
240-244	10643.0
245-249	26889.0
250-253	104899.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.16642805899586
#Duplication Level	Percentage of total
1	71.92232166656123
2	23.893767814379004
3	3.432950973292729
4	0.5009599834218749
5	0.09244211502219914
6	0.02006416054627056
7	0.023332194119680162
8	0.01333268235410295
9	0.0
>10	0.0
>50	0.0
>100	0.10082841030290356
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.725376586644986E-4	0.0
7	0.0	0.0	0.0	0.0	5.725376586644986E-4	0.0
8	0.0	0.0	0.0	0.0	5.725376586644986E-4	0.0
9	0.0	0.0	0.0	5.725376586644986E-4	5.725376586644986E-4	0.0
10-14	0.0	0.0	0.0	0.002061135571192195	0.00458030126931599	0.0
15-19	0.0	0.0	0.0	0.003091703356788293	0.014656964061811165	1.1450753173289973E-4
20-24	0.0	0.0	0.0	0.006755944372241083	0.020496848180189053	5.725376586644986E-4
25-29	0.0	0.0	0.0	0.1651198607588414	0.023474044005244443	5.725376586644986E-4
30-34	5.725376586644986E-4	0.0	0.0	2.0472801598525145	0.02690926995723144	5.725376586644986E-4
35-39	5.725376586644986E-4	0.0	0.0	6.176994291799543	0.028741390464957835	0.0011450753173289972
40-44	5.725376586644986E-4	0.0	0.0	13.133441352104933	0.031146048631348726	0.0011450753173289972
45-49	5.725376586644986E-4	0.0	0.0	19.443493395778106	0.034008736924671226	0.0011450753173289972
50-54	5.725376586644986E-4	0.0	0.0	25.548462450117654	0.03538282730546601	0.0011450753173289972
55-59	5.725376586644986E-4	0.0	0.0	30.894361076599814	0.03927608338438461	0.0011450753173289972
60-64	5.725376586644986E-4	0.0	0.0	35.904638127572845	0.06847550397627404	0.0028626882933224934
65-69	5.725376586644986E-4	0.0	0.0	40.46375550351824	0.07889568936396792	0.0028626882933224934
70-74	5.725376586644986E-4	0.0	0.0	44.87950944973406	0.08049879480822851	0.0028626882933224934
75-79	5.725376586644986E-4	0.0	0.0	48.936625806562425	0.08152936259382461	0.0028626882933224934
80-84	5.725376586644986E-4	0.0	0.0	52.75774214048929	0.083361483101551	0.0028626882933224934
85-89	5.725376586644986E-4	0.0	0.0	56.21586959882286	0.08496458854581161	0.0028626882933224934
90-94	5.725376586644986E-4	0.0	0.0	59.52170203995168	0.09286560823538167	0.0028626882933224934
95-99	5.725376586644986E-4	0.0	0.0	62.5505407618186	0.22180108896662679	0.0028626882933224934
100-104	5.725376586644986E-4	0.0	0.0	65.33605097875312	0.5444833133899383	0.0028626882933224934
105-109	5.725376586644986E-4	0.0	0.0	67.94865482277096	1.1171354795861699	0.0028626882933224934
110-114	5.725376586644986E-4	0.0	0.0	70.26960798346512	1.5802039379140163	0.0028626882933224934
115-119	5.725376586644986E-4	0.0	0.0	71.54980218823894	1.8835343894744674	0.0028626882933224934
120-124	5.725376586644986E-4	0.0	0.0	71.56846691591139	1.909413091646103	0.0028626882933224934
125-129	5.725376586644986E-4	0.0	0.0	71.57201664939512	1.9099856293047677	0.0028626882933224934
130-134	5.725376586644986E-4	0.0	0.0	71.57968865402123	1.9099856293047677	0.0028626882933224934
135-139	5.725376586644986E-4	0.0	0.0	71.58930728668678	1.9110161970903636	0.0028626882933224934
140-144	5.725376586644986E-4	0.0	0.0	71.60717046163711	1.9130773326615558	0.0028626882933224934
145-149	5.725376586644986E-4	0.0	0.0	71.6297284453885	1.913993392915419	0.0028626882933224934
150-154	5.725376586644986E-4	0.0	0.0	71.66236309193238	1.9151384682327481	0.0028626882933224934
155-159	5.725376586644986E-4	0.0	0.0	71.69786042676957	1.9167415736770086	0.0028626882933224934
160-164	5.725376586644986E-4	0.0	0.0	71.73564791224143	1.9182301715895362	0.0028626882933224934
165-169	5.725376586644986E-4	0.0	0.0	71.78213797012498	1.9191462318433996	0.0028626882933224934
170-174	5.725376586644986E-4	0.0	0.0	71.83687257029332	1.9191462318433996	0.0028626882933224934
175-179	5.725376586644986E-4	0.0	0.0	71.90431750648399	1.9191462318433996	0.0028626882933224934
180-184	5.725376586644986E-4	0.0	0.0	71.98401474857008	1.919718769502064	0.0028626882933224934
185-189	5.725376586644986E-4	0.0	0.0	72.05878816679167	1.9204058146924616	0.0028626882933224934
190-194	5.725376586644986E-4	0.0	0.0	72.12268336949862	1.921207367414592	0.0028626882933224934
195-199	5.725376586644986E-4	0.0	4.580301269315989E-4	72.20066299860873	1.9241845632396468	0.0028626882933224934
200-204	5.725376586644986E-4	0.0	5.725376586644986E-4	72.27784107499672	1.9374674369206637	0.0028626882933224934
205-209	5.725376586644986E-4	0.0	5.725376586644986E-4	72.33646893124396	1.9761709826463836	0.0028626882933224934
210-214	5.725376586644986E-4	0.0	5.725376586644986E-4	72.39601284774506	2.0117828250153154	0.0028626882933224934
215-219	5.725376586644986E-4	0.0	5.725376586644986E-4	72.45715986969043	2.053578074097824	0.0028626882933224934
220-224	5.725376586644986E-4	0.0	5.725376586644986E-4	72.5099478418193	2.1123204378768015	0.0028626882933224934
225-229	5.725376586644986E-4	0.0	5.725376586644986E-4	72.552086613497	2.172780414631773	0.0028626882933224934
230-234	0.0010305677855960975	0.0	5.725376586644986E-4	72.60224091239601	2.2103388850401635	0.0028626882933224934
235-239	0.0011450753173289972	0.0	5.725376586644986E-4	72.65090661338249	2.2320953160694144	0.0028626882933224934
240-241	0.0011450753173289972	0.0	5.725376586644986E-4	72.72659609185794	2.2340419441088737	0.0028626882933224934
>>END_MODULE
