##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665103
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3358301
Total Bases	833.8 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.222466657991646	32.0	25.0	33.0	18.0	33.0
2	30.643566791660426	33.0	29.0	33.0	27.0	33.0
3	29.62099377036186	31.0	29.0	33.0	18.0	33.0
4	30.833393433167544	33.0	31.0	33.0	27.0	33.0
5	30.35181926813588	32.0	31.0	33.0	27.0	33.0
6	32.37519001423637	35.0	29.0	37.0	16.0	38.0
7	35.07152426182168	37.0	35.0	38.0	29.0	38.0
8	34.656105870200435	37.0	34.0	38.0	29.0	38.0
9	35.885569816404185	38.0	36.0	38.0	31.0	38.0
10-14	36.414050259342446	38.0	37.4	38.0	33.6	38.0
15-19	36.58275080167025	38.0	38.0	38.0	34.0	38.0
20-24	36.7079047411176	38.0	38.0	38.0	34.0	38.0
25-29	36.69331081400982	38.0	38.0	38.0	34.0	38.0
30-34	36.67815940262651	38.0	38.0	38.0	34.0	38.0
35-39	36.65473267583817	38.0	38.0	38.0	34.0	38.0
40-44	36.620389774472265	38.0	38.0	38.0	34.0	38.0
45-49	36.60810320456683	38.0	38.0	38.0	34.0	38.0
50-54	36.55667154314041	38.0	38.0	38.0	34.0	38.0
55-59	36.481567018560874	38.0	38.0	38.0	34.0	38.0
60-64	36.43392006851083	38.0	38.0	38.0	34.0	38.0
65-69	36.3671139067046	38.0	37.2	38.0	33.4	38.0
70-74	36.298719263103564	38.0	37.0	38.0	33.0	38.0
75-79	36.19675273742786	38.0	37.0	38.0	33.0	38.0
80-84	36.0875435786695	38.0	37.0	38.0	33.0	38.0
85-89	35.9554918464985	38.0	37.0	38.0	31.4	38.0
90-94	35.854482637304535	38.0	37.0	38.0	31.0	38.0
95-99	35.7366832976637	38.0	36.4	38.0	31.0	38.0
100-104	35.61713915668407	38.0	36.0	38.0	30.6	38.0
105-109	35.4610297550254	38.0	36.0	38.0	29.0	38.0
110-114	35.31201505007905	38.0	35.2	38.0	29.0	38.0
115-119	35.148303659053504	38.0	35.0	38.0	28.0	38.0
120-124	35.19906751261719	38.0	35.0	38.0	29.0	38.0
125-129	33.6708177499224	34.2	33.4	35.6	31.2	35.6
130-134	35.219584858543826	36.2	36.0	37.0	32.8	37.2
135-139	36.51162148100803	38.0	38.0	38.0	34.0	38.0
140-144	36.46275714425958	38.0	38.0	38.0	34.0	38.0
145-149	36.448035867126634	38.0	38.0	38.0	34.0	38.0
150-154	36.43392480428321	38.0	38.0	38.0	34.0	38.0
155-159	36.42730021219086	38.0	38.0	38.0	34.0	38.0
160-164	36.41423518432034	38.0	38.0	38.0	34.0	38.0
165-169	36.368543143432795	38.0	38.0	38.0	33.4	38.0
170-174	36.34396234252009	38.0	38.0	38.0	33.0	38.0
175-179	36.30102974349856	38.0	38.0	38.0	33.0	38.0
180-184	36.243073153535605	38.0	37.6	38.0	33.0	38.0
185-189	36.164709928565244	38.0	37.0	38.0	33.0	38.0
190-194	36.07195054115227	38.0	37.0	38.0	33.0	38.0
195-199	35.974520107021576	38.0	37.0	38.0	31.4	38.0
200-204	35.82448745317742	38.0	37.0	38.0	31.0	38.0
205-209	35.69185437868079	38.0	37.0	38.0	30.2	38.0
210-214	35.57402639041696	38.0	37.0	38.0	29.0	38.0
215-219	35.39274330604992	38.0	36.0	38.0	28.6	38.0
220-224	35.234130912826295	38.0	36.0	38.0	27.8	38.0
225-229	35.02691852370289	38.0	35.6	38.0	26.6	38.0
230-234	34.82579177568694	38.0	35.0	38.0	25.2	38.0
235-239	34.66355955208939	38.0	35.0	38.0	24.2	38.0
240-244	34.581019586625274	38.0	34.2	38.0	24.0	38.0
245-249	34.82336058724023	38.0	34.2	38.0	26.6	38.0
250-252	35.8875334937138	38.0	34.666666666666664	38.0	31.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	9.0
18	15.0
19	41.0
20	191.0
21	757.0
22	2044.0
23	4508.0
24	8315.0
25	13712.0
26	20282.0
27	28865.0
28	38895.0
29	51603.0
30	66698.0
31	85685.0
32	111772.0
33	152324.0
34	225229.0
35	346876.0
36	665964.0
37	1534515.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.20098751192985	20.0441806736957	13.509420563553803	26.245411250820645
2	15.119563540817401	20.621457655038355	40.132769635094924	24.12620916904932
3	17.87290340101051	27.004826257125636	29.943161651027783	25.179108690836067
4	12.908883444322292	19.491247336443646	38.84185770623877	28.758011512995296
5	15.456522658338589	34.09327637557105	35.3557686409523	15.094432325138063
6	28.705182628332942	37.39589958717159	20.351749815978103	13.547167968517368
7	23.831663689466787	30.86718552029732	26.660802590357445	18.64034819987845
8	23.13104155940757	34.77151095151983	22.46528825141046	19.63215923766214
9	23.3327645930276	17.06203051008859	21.125843767931325	38.47936112895248
10-14	23.87956859021663	23.361433288903758	28.81933317281434	23.939664948065275
15-19	23.37692184232444	27.97789715692548	25.939235345491664	22.705945655258418
20-24	23.16424707779042	26.868468901120085	26.774415300513255	23.19286872057624
25-29	23.38069099339718	26.759677518389292	26.5347436707615	23.324887817452026
30-34	23.239507331601853	26.75786030739068	26.72386592587343	23.278766435134038
35-39	23.207212950302427	26.560963743657993	26.84690823744429	23.384915068595294
40-44	22.99170360969484	26.27549414396098	27.13247749751617	23.600324748828008
45-49	22.66673368862116	26.244181388567046	27.450385985709314	23.638698937102475
50-54	22.387923792973353	26.299154357016242	27.37185452165443	23.941067328355974
55-59	22.18520015529298	26.10801532943449	27.331647280553533	24.375137234718995
60-64	22.21360145635624	26.132245751063515	26.970128212519	24.68402458006124
65-69	22.252394886581044	26.19228592076767	26.532666369095566	25.02265282355572
70-74	22.362542880627693	26.34779555369534	26.17421246208323	25.115449103593733
75-79	22.40722928461291	26.56363199884036	25.887558556117902	25.141580160428827
80-84	22.32961838661297	26.66688861097544	25.799316645781467	25.20417635663012
85-89	22.284009721227882	26.409730804508612	25.926277968823914	25.379981505439588
90-94	22.28697437638381	26.148857888616405	26.00987184469489	25.554295890304896
95-99	22.16762657554426	25.935204646918645	26.20375837037901	25.69341040715808
100-104	22.045125220542378	26.036255103304335	26.275143379720266	25.643476296433022
105-109	21.877505060900564	26.705055611235156	26.043639121365953	25.373800206498327
110-114	21.636738209063004	27.40471695263907	25.768433867331307	25.190110970966618
115-119	21.52092717360982	27.654829529712526	25.727340965718852	25.096902330958805
120-124	21.566146152962173	27.877827813723233	25.60981308522625	24.94621294808834
125-129	22.98411592004005	25.45216532391905	27.691107686359732	23.872611069681163
130-134	21.748128720492748	31.04706989993023	27.462967699788283	19.74183367978874
135-139	24.155604291352	23.228307191632254	27.840847102196562	24.775241414819185
140-144	23.14663966467348	26.97489152078531	26.16740158994169	23.711067224599514
145-149	23.103013870733204	26.885286632858513	26.874250618284012	23.13744887812427
150-154	23.254617404583968	26.714694167085074	26.699791275455752	23.330897152875206
155-159	23.098155055640866	26.665356108858596	26.78644060224975	23.45004823325079
160-164	23.04253073882767	26.591449419598696	26.903030275737528	23.462989565836107
165-169	22.996221587526477	26.527563461051525	26.83618555129354	23.64002940012846
170-174	22.957412713162803	26.60185688006585	26.848888074411626	23.59184233235972
175-179	23.019929607931854	26.350892120237557	26.792639426343374	23.83653884548722
180-184	23.04162231321997	26.322321188430376	26.791499438635036	23.844557059714617
185-189	23.004710483647166	26.05852861659202	27.1342102590739	23.802550640686913
190-194	22.931603009582187	26.39013818295223	26.847650608702118	23.830608198763464
195-199	22.88814506376505	26.20110245442662	27.126365975500562	23.784386506307765
200-204	23.05199861323242	26.13845236401663	26.769460472614526	24.04008855013642
205-209	23.215957878263573	25.69851175234469	27.008964570522885	24.076565798868856
210-214	23.252230278228645	26.16493706683486	26.51217179081386	24.070660864122633
215-219	23.42871393211678	26.412094607353744	26.2353926305344	23.923798829995086
220-224	23.683700701140552	26.28135253367237	26.57543389119974	23.45951287398734
225-229	23.461903022314743	26.662744284550772	26.31216532014532	23.563187372989166
230-234	23.640032135698476	26.722497397192914	26.24368737638717	23.39378309072144
235-239	23.75437466841625	26.80608890953302	26.287887194822428	23.151649227228297
240-244	23.842642725109357	26.424196949336988	26.257532558180312	23.475627767373343
245-249	23.279841773171206	27.0365022331173	26.293580395991974	23.390075597719527
250-252	22.633433955105733	27.327721982530473	26.515936918941506	23.52290714342228
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.6666666666666666
14	1.6666666666666665
15	1.6666666666666665
16	1.3333333333333333
17	4.0
18	10.333333333333334
19	17.500000000000007
20	32.833333333333286
21	65.3333333333335
22	111.99999999999955
23	191.33333333333428
24	311.5000000000003
25	506.333333333323
26	799.0000000000188
27	1241.5000000000025
28	1905.1666666665215
29	2846.000000000149
30	4147.333333334063
31	6096.9999999990405
32	8806.666666664456
33	12357.500000004116
34	16913.333333344515
35	22837.999999989945
36	30006.499999964246
37	38273.33333332131
38	48013.66666672456
39	58698.500000134634
40	70200.16666680445
41	81530.33333330898
42	91350.83333316835
43	99161.8333330563
44	104422.1666663139
45	106290.33333295511
46	104555.99999964806
47	99075.16666639385
48	91102.66666650845
49	81355.16666664676
50	71192.16666679224
51	61706.333333487106
52	52677.83333342274
53	44654.66666669916
54	38069.666666652694
55	32482.49999995566
56	27947.9999999718
57	24686.999999983353
58	22002.833333326314
59	19817.000000000702
60	18000.000000006956
61	16288.33333334469
62	14749.500000008578
63	13240.666666672538
64	11662.16666666972
65	10020.166666666773
66	8393.33333333052
67	6885.666666664955
68	5440.166666666251
69	4144.000000000743
70	3114.166666666947
71	2245.166666666554
72	1612.333333333253
73	1075.00000000004
74	728.8333333333445
75	470.49999999999136
76	309.50000000000057
77	180.6666666666672
78	109.99999999999955
79	66.66666666666683
80	33.49999999999996
81	13.16666666666667
82	6.166666666666665
83	1.3333333333333333
84	1.1666666666666665
85	0.8333333333333333
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03504748383185426
2	8.93308848730355E-5
3	1.4888480812172582E-4
4	2.679926546191065E-4
5	6.848701173599389E-4
6	1.4888480812172582E-4
7	0.0
8	0.0
9	5.955392324869033E-5
10-14	8.218441408319266E-4
15-19	0.0
20-24	9.528627719790453E-5
25-29	0.0020605657444046853
30-34	0.0029479192008101717
35-39	0.002882409885236612
40-44	0.01139862090979933
45-49	0.005877972224645736
50-54	8.337549254816646E-4
55-59	1.0719706184764259E-4
60-64	5.3598530923821296E-5
65-69	0.0
70-74	7.325132559588911E-4
75-79	6.19361908349326E-4
80-84	0.0021916023871076133
85-89	0.0019772243997828623
90-94	0.005062228184402084
95-99	0.0025966685814119623
100-104	0.0017450707706601787
105-109	9.768488025084523E-4
110-114	4.289105998404095E-4
115-119	0.0011261004280194522
120-124	8.821380164530661E-4
125-129	0.0011501345507667295
130-134	0.0025518161275379686
135-139	0.002281623831681007
140-144	0.0013929913919736676
145-149	0.0014530341243863259
150-154	0.001915363163963793
155-159	0.001891346525245183
160-164	0.001999428631745955
165-169	0.0020474713128854393
170-174	0.0025218270132345484
175-179	0.0032003558459453175
180-184	0.00398106812958611
185-189	0.003116724016790675
190-194	0.0024626545938929412
195-199	0.001748504592949032
200-204	0.0017316256778442704
205-209	0.0022213326189752373
210-214	0.00481250498664168
215-219	0.013698060989573326
220-224	0.014766282727118002
225-229	0.0159011702196717
230-234	0.028661818026977638
235-239	0.07544912917083599
240-244	0.03250441995425965
245-249	1.781830482678689E-4
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	16.0
80-84	28.0
85-89	36.0
90-94	47.0
95-99	77.0
100-104	212.0
105-109	369.0
110-114	473.0
115-119	910.0
120-124	2788.0
125-129	22384.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	5.0
160-164	11.0
165-169	16.0
170-174	28.0
175-179	72.0
180-184	219.0
185-189	522.0
190-194	1000.0
195-199	1514.0
200-204	3400.0
205-209	5017.0
210-214	7739.0
215-219	11867.0
220-224	19306.0
225-229	30978.0
230-234	48068.0
235-239	68108.0
240-244	117644.0
245-249	370582.0
250-253	2644865.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.25871480404405
#Duplication Level	Percentage of total
1	79.83236182828884
2	13.183347857300243
3	3.6374545094185398
4	1.3343886979500024
5	0.6375961327991706
6	0.3524716899776923
7	0.23483795415734954
8	0.159968819780892
9	0.10858216686619462
>10	0.5101138019387508
>50	0.008876541522322564
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	2.9776961624345166E-5	0.0	0.0
2	0.0	0.0	0.0	8.933088487303551E-5	0.0	0.0
3	0.0	0.0	0.0	1.1910784649738066E-4	0.0	0.0
4	0.0	0.0	0.0	1.4888480812172585E-4	0.0	0.0
5	2.9776961624345166E-5	0.0	0.0	1.4888480812172585E-4	0.0	0.0
6	2.9776961624345166E-5	0.0	0.0	2.0843873137041616E-4	2.9776961624345166E-5	0.0
7	2.9776961624345166E-5	0.0	0.0	2.0843873137041616E-4	2.9776961624345166E-5	0.0
8	8.933088487303551E-5	0.0	0.0	2.3821569299476133E-4	2.9776961624345166E-5	0.0
9	8.933088487303551E-5	0.0	0.0	2.679926546191065E-4	2.9776961624345166E-5	0.0
10-14	1.250632388222497E-4	0.0	0.0	4.5260981669004657E-4	2.9776961624345163E-5	0.0
15-19	1.965279467206781E-4	0.0	0.0	7.265578636340222E-4	2.9776961624345163E-5	0.0
20-24	2.382156929947613E-4	0.0	0.0	0.0023762015376227446	2.9776961624345163E-5	0.0
25-29	2.382156929947613E-4	1.1910784649738066E-5	2.3821569299476133E-5	0.03383853918990585	2.9776961624345163E-5	0.0
30-34	2.382156929947613E-4	5.3598530923821296E-5	4.7643138598952266E-5	0.3255574768312906	2.322603006698923E-4	5.955392324869033E-6
35-39	2.382156929947613E-4	5.9553923248690326E-5	8.337549254816647E-5	1.0401509572846508	3.45412754842404E-4	2.9776961624345163E-5
40-44	2.977696162434517E-4	5.9553923248690326E-5	8.933088487303551E-5	2.4509536220845005	4.4665442436517754E-4	2.9776961624345163E-5
45-49	3.335019701926659E-4	7.146470789842841E-5	1.1910784649738068E-4	4.098649882782991	4.764313859895227E-4	5.9553923248690326E-5
50-54	3.8114510879161807E-4	8.933088487303551E-5	1.7866176974607102E-4	5.908690138257411	6.967809020096769E-4	8.933088487303551E-5
55-59	4.109220704159633E-4	8.933088487303551E-5	1.7866176974607102E-4	7.772281281517053	0.001101747580100771	1.1315245417251164E-4
60-64	4.764313859895226E-4	8.933088487303551E-5	1.7866176974607102E-4	9.71249450242846	0.0014650265119177822	1.4888480812172585E-4
65-69	4.942975629641298E-4	8.933088487303551E-5	1.7866176974607102E-4	11.688702114551377	0.0016913314202628056	1.6079559277146392E-4
70-74	5.062083476138679E-4	8.933088487303551E-5	1.7866176974607102E-4	13.750268364866638	0.0019235917209326978	1.7866176974607102E-4
75-79	5.062083476138679E-4	8.933088487303551E-5	2.0248333904554716E-4	15.829837766179983	0.0022273167295010186	1.7866176974607102E-4
80-84	5.18119132263606E-4	8.933088487303551E-5	2.0843873137041616E-4	17.971075255017343	0.0025250863457444697	1.7866176974607102E-4
85-89	5.598068785376891E-4	8.933088487303551E-5	2.0843873137041616E-4	20.142387475095294	0.0029181422391858264	1.7866176974607102E-4
90-94	5.955392324869034E-4	1.1315245417251164E-4	2.0843873137041616E-4	22.32412758713409	0.004835778567793656	1.7866176974607102E-4
95-99	5.955392324869034E-4	1.7866176974607102E-4	2.0843873137041616E-4	24.514026586657955	0.030551162626578143	2.0843873137041616E-4
100-104	5.955392324869034E-4	1.7866176974607102E-4	2.2034951602015423E-4	26.700810915995913	0.09556618063717338	2.382156929947613E-4
105-109	6.193608017863796E-4	1.7866176974607102E-4	2.382156929947613E-4	28.883557489337612	0.21349485945423	2.382156929947613E-4
110-114	6.491377634107248E-4	1.7866176974607102E-4	2.382156929947613E-4	31.03932018005533	0.322490449783983	2.620372622942375E-4
115-119	6.550931557355937E-4	1.7866176974607102E-4	2.382156929947613E-4	32.2945918188989	0.4031383726473594	2.679926546191065E-4
120-124	6.550931557355937E-4	1.7866176974607102E-4	2.441710853196304E-4	32.32279655694948	0.41391167736304757	2.679926546191065E-4
125-129	6.789147250350698E-4	1.7866176974607102E-4	2.977696162434517E-4	32.323028817250155	0.4139593205016466	2.679926546191065E-4
130-134	7.027362943345459E-4	1.7866176974607102E-4	2.977696162434517E-4	32.32348738245916	0.4140069636402455	2.799034392688446E-4
135-139	8.218441408319266E-4	1.7866176974607102E-4	3.45412754842404E-4	32.32447002219277	0.4140367406018698	3.2754657786779686E-4
140-144	8.575764947811408E-4	1.905725543958091E-4	3.5732353949214203E-4	32.32591122713539	0.41407247295581906	3.8710050111648715E-4
145-149	9.111750257049621E-4	2.0843873137041616E-4	3.5732353949214203E-4	32.32795392670282	0.4140784283481439	3.8710050111648715E-4
150-154	9.528627719790452E-4	2.0843873137041616E-4	3.5732353949214203E-4	32.33143783121287	0.4141379822713926	3.930558934413562E-4
155-159	9.528627719790452E-4	2.0843873137041616E-4	3.692343241418801E-4	32.33663093332015	0.4141975361946413	4.2878824739057037E-4
160-164	9.528627719790452E-4	2.0843873137041616E-4	3.8710050111648715E-4	32.34315208791588	0.41425709011788997	4.7047599366465364E-4
165-169	9.528627719790452E-4	2.0843873137041616E-4	3.8710050111648715E-4	32.3504355327292	0.41429877786416397	5.121637399387369E-4
170-174	9.528627719790452E-4	2.0843873137041616E-4	3.8710050111648715E-4	32.35913040552351	0.4143642871797376	5.538514862128201E-4
175-179	9.826397336033905E-4	2.0843873137041616E-4	3.8710050111648715E-4	32.36921884012184	0.4144774396339101	5.657622708625582E-4
180-184	0.0010243274798774737	2.0843873137041616E-4	3.8710050111648715E-4	32.382487454221646	0.4146084582650572	5.717176631874273E-4
185-189	0.00104814904917695	2.0843873137041616E-4	4.109220704159633E-4	32.39803698358188	0.4148704955273515	6.312715864361176E-4
190-194	0.001071970618476426	2.0843873137041616E-4	4.4665442436517754E-4	32.4139914796202	0.415209952889869	6.729593327102007E-4
195-199	0.0010898367954510332	2.0843873137041616E-4	4.4665442436517754E-4	32.43077377519168	0.41594842153815276	6.848701173599389E-4
200-204	0.0011077029724256401	2.0843873137041616E-4	4.7047599366465364E-4	32.44868759530489	0.4211593898224132	6.848701173599389E-4
205-209	0.0011613015033494615	2.560818699693684E-4	4.764313859895226E-4	32.46457658202764	0.4351069186472564	6.848701173599389E-4
210-214	0.0012149000342732827	2.679926546191065E-4	4.942975629641298E-4	32.48073951679733	0.45558155746015616	6.848701173599389E-4
215-219	0.00123276621124789	2.679926546191065E-4	5.181191322636059E-4	32.50304841644629	0.48292276362362985	7.08691686659415E-4
220-224	0.0012506323882224971	2.679926546191065E-4	5.657622708625582E-4	32.528787622074375	0.521841252466649	7.444240406086292E-4
225-229	0.0012506323882224971	2.679926546191065E-4	5.717176631874273E-4	32.55268065608175	0.5718605926032241	7.444240406086292E-4
230-234	0.001256587780547366	2.679926546191065E-4	6.193608017863796E-4	32.57445357042148	0.6164367041548687	7.444240406086292E-4
235-239	0.0013161417037960564	2.679926546191065E-4	6.253161941112486E-4	32.60393276242958	0.6492985590034961	7.444240406086292E-4
240-241	0.0013697402347198778	2.679926546191065E-4	6.253161941112486E-4	32.67248826117731	0.6503437303565106	7.444240406086292E-4
>>END_MODULE
