##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665112
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2844305
Total Bases	701.8 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.308364609280652	31.0	18.0	33.0	18.0	33.0
2	29.672795990584696	31.0	29.0	33.0	25.0	33.0
3	31.132615173126652	33.0	31.0	33.0	27.0	33.0
4	30.634354613868766	33.0	31.0	33.0	27.0	33.0
5	31.504720485320668	33.0	32.0	33.0	29.0	33.0
6	34.419061598527584	37.0	34.0	38.0	28.0	38.0
7	35.87796561901765	38.0	36.0	38.0	31.0	38.0
8	35.818092644776144	38.0	36.0	38.0	31.0	38.0
9	36.41897932886944	38.0	37.0	38.0	34.0	38.0
10-14	36.66300526842234	38.0	37.8	38.0	34.0	38.0
15-19	36.61248248693441	38.0	38.0	38.0	34.0	38.0
20-24	36.63504560868121	38.0	38.0	38.0	34.0	38.0
25-29	36.58670937188522	38.0	38.0	38.0	34.0	38.0
30-34	36.55984980513693	38.0	38.0	38.0	34.0	38.0
35-39	36.49440654219572	38.0	38.0	38.0	34.0	38.0
40-44	36.50842550289086	38.0	38.0	38.0	34.0	38.0
45-49	36.45473695683128	38.0	37.8	38.0	34.0	38.0
50-54	36.335502697495514	38.0	37.0	38.0	33.6	38.0
55-59	36.219112999484935	38.0	37.0	38.0	33.0	38.0
60-64	36.16443925669012	38.0	37.0	38.0	33.0	38.0
65-69	36.08452272171936	38.0	37.0	38.0	32.6	38.0
70-74	35.95720592552486	38.0	37.0	38.0	31.6	38.0
75-79	35.79272780984468	38.0	36.8	38.0	30.8	38.0
80-84	35.578832315667	38.0	36.0	38.0	29.0	38.0
85-89	35.402695756703864	38.0	36.0	38.0	29.0	38.0
90-94	35.28428392684511	38.0	36.0	38.0	28.0	38.0
95-99	35.12240508585274	38.0	35.4	38.0	27.6	38.0
100-104	34.937376889038624	38.0	35.0	38.0	26.8	38.0
105-109	34.67195241754173	38.0	34.4	38.0	26.0	38.0
110-114	34.494454089389464	38.0	34.0	38.0	25.2	38.0
115-119	34.33057518391712	38.0	34.0	38.0	24.6	38.0
120-124	34.61744292877412	38.0	34.0	38.0	27.0	38.0
125-129	33.866971028619474	34.4	33.2	35.6	31.0	38.0
130-134	35.44464823503424	36.4	36.0	37.2	33.0	38.0
135-139	36.501210408659155	38.0	38.0	38.0	34.0	38.0
140-144	36.433102929092115	38.0	38.0	38.0	34.0	38.0
145-149	36.41634304395847	38.0	38.0	38.0	34.0	38.0
150-154	36.40217455640184	38.0	38.0	38.0	34.0	38.0
155-159	36.39778183845145	38.0	38.0	38.0	34.0	38.0
160-164	36.36292271703762	38.0	38.0	38.0	33.8	38.0
165-169	36.32784387998964	38.0	38.0	38.0	33.2	38.0
170-174	36.27781401838803	38.0	37.2	38.0	33.0	38.0
175-179	36.21252031975125	38.0	37.0	38.0	33.0	38.0
180-184	36.085987539869144	38.0	37.0	38.0	32.6	38.0
185-189	35.962986587889944	38.0	37.0	38.0	31.0	38.0
190-194	35.84498989566546	38.0	37.0	38.0	31.0	38.0
195-199	35.67816896610505	38.0	37.0	38.0	29.4	38.0
200-204	35.46671311515197	38.0	36.2	38.0	29.0	38.0
205-209	35.23410177240464	38.0	36.0	38.0	27.8	38.0
210-214	34.956269423931914	38.0	35.4	38.0	26.2	38.0
215-219	34.636056016253505	38.0	35.0	38.0	24.6	38.0
220-224	34.39831427578956	38.0	34.0	38.0	23.2	38.0
225-229	34.119445418496234	38.0	34.0	38.0	23.0	38.0
230-234	33.89132256297752	38.0	34.0	38.0	23.0	38.0
235-239	33.64408908258157	38.0	33.2	38.0	21.0	38.0
240-244	33.50701145445287	38.0	33.0	38.0	21.0	38.0
245-249	33.7983542518728	38.0	33.0	38.0	22.6	38.0
250-252	35.35308032008616	37.666666666666664	33.666666666666664	38.0	30.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.0
17	14.0
18	27.0
19	44.0
20	167.0
21	607.0
22	1788.0
23	4191.0
24	8016.0
25	13309.0
26	20369.0
27	29102.0
28	39600.0
29	52103.0
30	67752.0
31	86428.0
32	111792.0
33	148917.0
34	211594.0
35	338828.0
36	689194.0
37	1020457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.861374641067506	21.663089071561288	12.413222791509487	22.06231349586172
2	15.565063093007648	22.106126197265894	42.23678784147765	20.092022868248804
3	15.737015934358404	31.203244109082956	33.049115175857345	20.010624780701292
4	11.01074642776028	21.605115364304815	44.200599516083784	23.183538691851126
5	11.563005946992137	39.059942797775896	38.45757911890293	10.919472136329038
6	22.04954182112742	46.22754811018794	21.17013511917151	10.552774949513132
7	20.827992245549172	35.50301620573343	28.687565525742343	14.981426022975056
8	20.502197377210564	39.058081074429566	23.697781527968218	16.74194002039166
9	22.499607109369148	17.821800279365455	25.109315006171983	34.569277605093404
10-14	21.111564180706843	25.93541937075735	31.846873633451978	21.106142815083828
15-19	20.323533789970167	31.231536335128336	28.504154234503744	19.940775640397757
20-24	20.24027504364533	30.010170550798893	29.622263605713396	20.127290799842378
25-29	20.281291196868256	29.937415215491527	29.65360948905828	20.127684098581934
30-34	20.199172970967634	29.708347384820232	29.81255574303372	20.279923901178414
35-39	19.96862920364206	28.700720676085872	30.420918120702677	20.909731999569384
40-44	19.438018927805913	27.956396475733136	31.126446409245823	21.479138187215128
45-49	18.432167069603285	27.65352082132973	31.91574459551384	21.99856751355314
50-54	17.612021635061627	27.92766655228473	31.310008325504736	23.150303487148907
55-59	17.346003483379484	27.098003431063393	30.584751112191498	24.971241973365625
60-64	18.043187147587684	26.95422095976252	28.729646329708437	26.272945562941363
65-69	18.086861132939198	26.7545228087385	27.551915392580327	27.60670066574198
70-74	18.523938349107986	26.633033452015354	26.711387496686022	28.131640702190637
75-79	18.72235112664434	26.00504309542767	26.509851940647987	28.762753837280002
80-84	18.887705891026982	25.570094451608767	26.57805222765623	28.96414742970802
85-89	19.20802152134692	24.392528733651954	27.21034596407591	29.189103780925212
90-94	19.542158514715815	23.168713520571373	27.660501047558466	29.62862691715435
95-99	19.370708897785956	22.409853890329774	28.747798763102118	29.47163844878215
100-104	19.244605417021507	23.438672629362987	28.74372303183233	28.572998921783178
105-109	18.661137536606123	26.477281464313908	27.68933625467163	27.172244744408342
110-114	18.01557486534605	29.191717467144297	26.862452979729877	25.93025468777978
115-119	17.926441990888982	30.12920237802247	26.78336729901698	25.16098833207157
120-124	18.02524933430699	30.62951107922854	26.853271241181858	24.491968345282608
125-129	19.950792914236153	28.48427767571916	29.62394401113675	21.940985398907937
130-134	18.308714081718893	34.03235902009046	30.462036682021488	17.19689021616916
135-139	21.225891991455914	25.811916265683926	31.055784349107185	21.906407393752975
140-144	19.864215577670546	29.666983324494346	29.457187498807052	21.011613599028056
145-149	19.77956855197048	29.392848561256685	30.255541025044124	20.57204186172871
150-154	19.617066511855803	29.065055838163456	30.55449769547528	20.763379954505456
155-159	19.44497879075227	28.604683107660094	30.620355207974768	21.329982893612865
160-164	19.260276105162625	28.368186123365223	30.75475074273492	21.61678702873723
165-169	19.184224898065228	28.233784550453073	30.200264049501328	22.38172650198037
170-174	19.17323374324673	28.03296358908895	30.11522721197739	22.67857545568693
175-179	19.359199424176783	27.151399412030823	29.95882745233364	23.530573711458754
180-184	19.651576142065053	26.386628276693703	29.706688852106396	24.255106729134855
185-189	19.05696895909184	25.45385578299398	30.814709389408968	24.674465868505216
190-194	19.07219782673783	25.809761704305046	29.93798538854348	25.180055080413645
195-199	19.14844494367081	25.24078987482166	30.47328969975448	25.137475481753047
200-204	20.21810269032338	24.491582888583885	29.296639206606944	25.99367521448579
205-209	20.878630828798123	23.697300157441394	29.352436969062172	26.071632044698305
210-214	21.076723409641414	24.104643532981417	28.172566622482048	26.646066434895115
215-219	20.432975375301183	24.512039834626705	28.221250548318043	26.83373424175407
220-224	20.66981949330068	24.860726298238255	28.961295163366714	25.50815904509436
225-229	20.23764167280704	26.13170981397717	28.19594898521708	25.43469952799871
230-234	21.02025953725261	26.433787436187746	28.00774802172658	24.53820500483306
235-239	21.497390437059565	25.875537718635893	27.75346707400821	24.873604770296332
240-244	21.29650806383879	25.81180252806046	28.022370443194404	24.869318964906345
245-249	20.786433893723366	27.26747385775609	27.768150072842506	24.177942175678037
250-252	18.794976786286437	29.06409531566706	27.661561195187346	24.479366702859163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	0.0
8	0.0
9	0.3333333333333333
10	0.3333333333333333
11	0.3333333333333333
12	2.333333333333333
13	2.6666666666666665
14	9.166666666666664
15	21.66666666666666
16	50.00000000000007
17	78.99999999999997
18	146.66666666666643
19	260.1666666666694
20	406.3333333333278
21	632.6666666666684
22	969.5000000000398
23	1435.999999999956
24	2045.8333333331534
25	2901.833333333521
26	3982.6666666673423
27	5389.166666666276
28	7361.833333331163
29	10162.50000000046
30	13740.166666673585
31	18051.33333333968
32	23093.833333321498
33	29602.833333297913
34	37419.66666665128
35	46542.66666671724
36	56216.500000120664
37	65765.66666685647
38	74040.16666673982
39	80815.16666664243
40	85809.16666657076
41	87016.99999988705
42	84627.66666658834
43	79513.83333332934
44	72943.16666676034
45	67016.00000018162
46	60693.333333482944
47	54832.333333441675
48	49900.83333340608
49	44876.83333336983
50	39994.66666666863
51	36583.33333331081
52	33479.499999954955
53	29746.49999996459
54	25258.499999980744
55	21518.33333332782
56	18132.5000000071
57	14921.666666675064
58	12164.166666670071
59	9912.166666665971
60	7508.99999999806
61	5682.666666666368
62	4329.666666667374
63	3242.83333333361
64	2489.8333333333067
65	1780.9999999998845
66	1232.666666666674
67	859.000000000026
68	642.6666666666692
69	486.6666666666568
70	387.49999999999574
71	312.3333333333333
72	236.50000000000225
73	174.00000000000054
74	122.83333333333269
75	105.9999999999996
76	74.83333333333337
77	41.33333333333334
78	22.83333333333332
79	15.333333333333341
80	10.499999999999998
81	3.5
82	1.3333333333333333
83	0.6666666666666666
84	0.6666666666666666
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08947704272221157
2	4.2189568277663616E-4
3	3.8673770921191647E-4
4	3.8673770921191647E-4
5	3.515797356471968E-4
6	3.5157973564719675E-5
7	1.0547392069415904E-4
8	1.757898678235984E-4
9	7.031594712943935E-5
10-14	1.1250551540710296E-4
15-19	8.437913655532723E-5
20-24	5.133064140449073E-4
25-29	0.0012445922641910764
30-34	1.1953711012004692E-4
35-39	1.2656870483299083E-4
40-44	9.844232598121509E-5
45-49	6.961278765814496E-4
50-54	0.0011602131276357492
55-59	0.001954783330198414
60-64	0.0018071198412265913
65-69	0.0038673770921191643
70-74	0.010230970307333427
75-79	0.011489668580017596
80-84	0.002897053689366231
85-89	5.695752318874373E-4
90-94	0.0011602681161626683
95-99	1.8986752942472038E-4
100-104	0.002433330780445838
105-109	4.8537105702842616E-4
110-114	9.779487335352833E-4
115-119	0.002026850562067478
120-124	0.004731465066473916
125-129	0.030502335335049088
130-134	0.021191959354981225
135-139	0.010037552462999779
140-144	0.008602606582725868
145-149	0.008213828142257565
150-154	0.010171859899488735
155-159	0.013084175569702393
160-164	0.015275491834079743
165-169	0.012038076797416343
170-174	0.010539579833394054
175-179	0.012957312547344028
180-184	0.006560185709808913
185-189	0.007069758366263678
190-194	0.006081019258022314
195-199	0.0045904397620072715
200-204	0.0026327219166951583
205-209	3.826021723159413E-4
210-214	7.742073874300682E-4
215-219	6.505425989948903E-4
220-224	8.314347099988267E-4
225-229	5.073043369447765E-4
230-234	1.96603849972207E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	23.0
80-84	36.0
85-89	53.0
90-94	65.0
95-99	140.0
100-104	632.0
105-109	432.0
110-114	675.0
115-119	1041.0
120-124	2265.0
125-129	9568.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	3.0
160-164	7.0
165-169	18.0
170-174	34.0
175-179	64.0
180-184	181.0
185-189	353.0
190-194	695.0
195-199	1150.0
200-204	2564.0
205-209	4525.0
210-214	12641.0
215-219	26182.0
220-224	39391.0
225-229	60046.0
230-234	97016.0
235-239	116611.0
240-244	165988.0
245-249	420035.0
250-253	1881870.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.833176380065204
#Duplication Level	Percentage of total
1	32.808326593008005
2	13.055494738923334
3	6.662476521449685
4	4.43014234359538
5	3.310685206404804
6	2.617832058076964
7	2.224250044329554
8	2.041973954425044
9	1.6974602080969279
>10	22.344270780548836
>50	5.499873968546293
>100	3.1969490267564655
>500	0.11026455583860904
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.5157973564719675E-5	0.0
7	0.0	0.0	0.0	1.757898678235984E-4	3.5157973564719675E-5	0.0
8	0.0	0.0	0.0	1.757898678235984E-4	3.5157973564719675E-5	0.0
9	0.0	0.0	0.0	2.812637885177574E-4	3.5157973564719675E-5	0.0
10-14	0.0	0.0	0.0	3.023585726565892E-4	6.328435241649541E-5	0.0
15-19	0.0	0.0	0.0	7.945702025626648E-4	2.390742202400938E-4	0.0
20-24	0.0	0.0	0.0	0.00825509219299618	3.2345335679542103E-4	0.0
25-29	0.0	0.0	0.0	0.08790196550651212	4.781484404801876E-4	0.0
30-34	0.0	0.0	0.0	1.2246998827481579	0.0013711609690240673	3.5157973564719675E-5
35-39	0.0	0.0	0.0	4.280033259442993	0.0014063189425887871	3.5157973564719675E-5
40-44	0.0	0.0	0.0	9.829459217629614	0.0015188244579958901	3.5157973564719675E-5
45-49	0.0	0.0	0.0	15.604332165502644	0.0017086775152453763	3.5157973564719675E-5
50-54	0.0	0.0	0.0	21.386082013004934	0.0019618149249113577	3.5157973564719675E-5
55-59	0.0	0.0	0.0	26.878608306774414	0.002432931770678602	3.5157973564719675E-5
60-64	0.0	0.0	0.0	32.12031058553847	0.003150154431398883	3.5157973564719675E-5
65-69	0.0	0.0	0.0	37.01385048368582	0.0036915872242955666	3.5157973564719675E-5
70-74	0.0	0.0	0.0	41.603351258040185	0.004015040581090987	3.5157973564719675E-5
75-79	0.0	0.0	0.0	45.8711706374668	0.004387715100877016	3.5157973564719675E-5
80-84	0.0	0.0	0.0	49.820107196661404	0.004739294836524212	3.5157973564719675E-5
85-89	0.0	0.0	0.0	53.48548063586711	0.005421359523679774	3.5157973564719675E-5
90-94	0.0	1.406318942588787E-5	0.0	56.84712434144721	0.01054036047470296	3.5157973564719675E-5
95-99	0.0	3.5157973564719675E-5	0.0	59.940632245838614	0.1108460590548482	3.5157973564719675E-5
100-104	0.0	3.5157973564719675E-5	0.0	62.795023740421655	0.4380191294534166	3.5157973564719675E-5
105-109	0.0	3.5157973564719675E-5	0.0	65.42586677589077	1.0582479727033494	6.328435241649541E-5
110-114	0.0	3.5157973564719675E-5	0.0	67.81575815533145	1.6581976968011518	7.031594712943935E-5
115-119	0.0	3.5157973564719675E-5	0.0	69.14404045979599	2.096104320739161	7.031594712943935E-5
120-124	1.406318942588787E-5	3.5157973564719675E-5	0.0	69.17913514900829	2.1461622435006094	8.437913655532723E-5
125-129	6.328435241649541E-5	3.5157973564719675E-5	0.0	69.17995784558968	2.146422412504988	1.0547392069415903E-4
130-134	7.031594712943935E-5	3.5157973564719675E-5	0.0	69.18266500955419	2.146506791641543	1.0547392069415903E-4
135-139	7.031594712943935E-5	3.5157973564719675E-5	0.0	69.19013256313931	2.146556012804534	1.0547392069415903E-4
140-144	7.031594712943935E-5	3.5157973564719675E-5	0.0	69.20246598026583	2.146795087024774	1.2656870483299083E-4
145-149	7.031594712943935E-5	3.5157973564719675E-5	7.031594712943935E-6	69.21757687730396	2.1468794661613293	1.406318942588787E-4
150-154	7.031594712943935E-5	4.218956827766361E-5	3.5157973564719675E-5	69.23670984651787	2.1470130664608753	1.9688465196243018E-4
155-159	7.031594712943935E-5	7.031594712943935E-5	3.5157973564719675E-5	69.26203061907918	2.1472802670599673	2.1094784138831805E-4
160-164	7.031594712943935E-5	7.031594712943935E-5	3.5157973564719675E-5	69.29360247934031	2.147688099553318	2.1094784138831805E-4
165-169	7.031594712943935E-5	7.031594712943935E-5	3.5157973564719675E-5	69.33051131998853	2.1478990473947066	2.1094784138831805E-4
170-174	7.031594712943935E-5	7.031594712943935E-5	4.218956827766361E-5	69.37537992585185	2.1480467108836785	2.1094784138831805E-4
175-179	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	69.43029668055992	2.1482014059673626	2.1094784138831805E-4
180-184	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	69.50183612516942	2.1484826697558805	2.3204262552714984E-4
185-189	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	69.57128015455446	2.1487569019496857	2.461058149530377E-4
190-194	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	69.6355700250149	2.1494389666368408	2.601690043789256E-4
195-199	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	69.69884734583668	2.1512179600992156	3.515797356471968E-4
200-204	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	69.75923468123145	2.158713640063214	3.515797356471968E-4
205-209	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	69.81500225889981	2.189448740553492	3.515797356471968E-4
210-214	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	69.86738060791653	2.242227890468849	3.515797356471968E-4
215-219	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	69.9135992799647	2.306763866744249	3.586113303601407E-4
220-224	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	69.95016357247201	2.395748697836554	3.937693039248604E-4
225-229	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	69.98844357408927	2.490365836293928	4.218956827766361E-4
230-234	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	70.03274965237554	2.557306617961154	4.218956827766361E-4
235-239	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	70.0822731739388	2.594236553393536	4.218956827766361E-4
240-241	7.031594712943935E-5	7.031594712943935E-5	7.031594712943935E-5	70.15620687654805	2.596258136873507	4.218956827766361E-4
>>END_MODULE
