##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665117
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26765
Total Bases	6.5 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.10487577059593	25.0	18.0	32.0	18.0	33.0
2	26.999551653278534	29.0	25.0	31.0	18.0	33.0
3	28.62529422753596	30.0	27.0	33.0	18.0	33.0
4	30.53629740332524	31.0	30.0	33.0	27.0	33.0
5	30.519260227909584	33.0	31.0	33.0	27.0	33.0
6	34.501550532411734	37.0	34.0	38.0	28.0	38.0
7	34.940556697179154	37.0	34.0	38.0	29.0	38.0
8	35.878348589575936	38.0	36.0	38.0	31.0	38.0
9	36.215617410797684	38.0	37.0	38.0	33.0	38.0
10-14	36.47056977395853	38.0	37.6	38.0	33.8	38.0
15-19	36.549060339996274	38.0	38.0	38.0	34.0	38.0
20-24	36.568802540631424	38.0	38.0	38.0	34.0	38.0
25-29	36.506781244162156	38.0	38.0	38.0	34.0	38.0
30-34	36.48068746497292	38.0	38.0	38.0	34.0	38.0
35-39	36.45968989351765	38.0	38.0	38.0	34.0	38.0
40-44	36.41807584532038	38.0	38.0	38.0	33.8	38.0
45-49	36.410439006164765	38.0	38.0	38.0	33.8	38.0
50-54	36.313984681487014	38.0	37.2	38.0	33.0	38.0
55-59	36.24109097702223	38.0	37.0	38.0	33.0	38.0
60-64	36.171896133009525	38.0	37.0	38.0	33.0	38.0
65-69	36.056424434896314	38.0	37.0	38.0	32.4	38.0
70-74	35.92852606015318	38.0	37.0	38.0	31.4	38.0
75-79	35.78137668038896	38.0	37.0	38.0	31.0	38.0
80-84	35.59341851752731	38.0	36.0	38.0	29.4	38.0
85-89	35.41121631284098	38.0	36.0	38.0	29.0	38.0
90-94	35.275840826832464	38.0	36.0	38.0	28.2	38.0
95-99	35.12430987449872	38.0	35.0	38.0	27.4	38.0
100-104	34.9845985020522	38.0	35.0	38.0	26.8	38.0
105-109	34.77453751904663	38.0	34.8	38.0	25.6	38.0
110-114	34.58323493956661	38.0	34.0	38.0	24.6	38.0
115-119	34.44068814817062	38.0	34.0	38.0	24.2	38.0
120-124	34.55847094510619	38.0	34.0	38.0	25.8	38.0
125-129	33.37438772634968	34.2	33.2	35.6	29.8	36.4
130-134	34.73173657269463	36.2	35.4	37.2	30.4	38.0
135-139	35.88351287340515	38.0	37.0	38.0	31.0	38.0
140-144	35.82504393001757	38.0	37.0	38.0	30.4	38.0
145-149	35.797402398960955	38.0	37.0	38.0	29.4	38.0
150-154	35.750851860340745	38.0	37.0	38.0	29.0	38.0
155-159	35.75322790129116	38.0	37.0	38.0	29.0	38.0
160-164	35.729709286778466	38.0	37.0	38.0	29.0	38.0
165-169	35.67286158351351	38.0	37.0	38.0	29.0	38.0
170-174	35.66030156117709	38.0	37.0	38.0	29.0	38.0
175-179	35.62073662023627	38.0	37.0	38.0	29.0	38.0
180-184	35.51480936717576	38.0	37.0	38.0	29.0	38.0
185-189	35.39550186025596	38.0	36.4	38.0	28.2	38.0
190-194	35.265292993640244	38.0	36.0	38.0	27.6	38.0
195-199	35.109764459468195	38.0	36.0	38.0	27.0	38.0
200-204	34.88630226518745	38.0	35.4	38.0	25.6	38.0
205-209	34.75804467367224	38.0	35.0	38.0	25.0	38.0
210-214	34.5755881852039	38.0	35.0	38.0	23.6	38.0
215-219	34.32283765646066	38.0	34.2	38.0	23.0	38.0
220-224	34.17839923525994	38.0	34.0	38.0	23.0	38.0
225-229	33.956160575530006	38.0	34.0	38.0	21.4	38.0
230-234	33.84481196392918	38.0	34.0	38.0	21.0	38.0
235-239	33.686432737114295	38.0	33.2	38.0	21.0	38.0
240-244	33.60628038122918	38.0	33.0	38.0	21.0	38.0
245-249	33.96674609098555	38.0	33.0	38.0	22.8	38.0
250-252	35.50282612802069	37.333333333333336	33.666666666666664	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	4.0
19	7.0
20	6.0
21	19.0
22	34.0
23	86.0
24	170.0
25	210.0
26	351.0
27	419.0
28	558.0
29	583.0
30	686.0
31	804.0
32	1013.0
33	1392.0
34	1979.0
35	2982.0
36	5583.0
37	9876.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.74750569859123	26.15373117596502	11.595231867269534	25.50353125817421
2	16.53652157668597	19.23407435083131	35.45301699981319	28.77638707266953
3	18.531664487203436	25.544554455445546	30.132635905099942	25.791145152251072
4	16.095647300579113	18.789463852045582	35.845320381094716	29.26956846628059
5	17.9562861946572	32.837661124603024	32.02690080328788	17.179151877451897
6	30.85746310480105	35.621147020362415	18.703530730431535	14.817859144405007
7	25.87334205118625	29.35550158789464	24.748739024845882	20.022417336073232
8	24.774892583597982	32.68821221744816	20.93779189239679	21.599103306557073
9	24.45731365589389	16.260041098449467	19.734728189800112	39.54791705585653
10-14	25.61218923503434	21.810899471690217	26.595577740747107	25.98133355252834
15-19	25.30767793760508	26.119932747991783	24.044834672146457	24.52755464225668
20-24	25.001307677937607	25.178404632916124	24.694190173734356	25.126097515411917
25-29	25.028209324396023	25.026714790653187	24.6366414837731	25.308434401177692
30-34	25.053616398024225	24.957966238482747	24.854843410227094	25.13357395326593
35-39	24.659243760274997	24.28037662531759	25.563443431475115	25.496936182932295
40-44	24.006277323170046	24.07876545977656	26.071068265889473	25.84388895116392
45-49	23.408309669705574	24.130922134210135	26.764310267523538	25.69645792856075
50-54	22.510909851745573	24.53819942611191	26.601357006217118	26.349533715925393
55-59	22.399569583931132	24.20417862266858	26.19784194165471	27.198409851745573
60-64	22.799348397895745	24.52624342419895	25.219691535150645	27.45471664275466
65-69	22.849990659443304	24.621707453764245	24.13450401643938	28.393797870353072
70-74	23.18211368748272	25.355133273054708	23.400312352884033	28.062440686578537
75-79	23.38504767883299	25.84521567572415	23.182524736197053	27.587211909245806
80-84	23.296783789642053	25.826849339641676	23.390213093556365	27.4861537771599
85-89	23.281004709576138	25.373402108095988	23.70262390670554	27.642969275622338
90-94	23.219399848952765	24.358983945622995	24.378425669057002	28.04319053636724
95-99	22.85975044136569	24.18384751189443	25.07256351177474	27.88383853496514
100-104	22.656828777322964	25.015527601715142	25.00954105647557	27.318102564486317
105-109	22.117558966679145	26.74279296143766	24.567577686259828	26.572070385623363
110-114	21.886662370168313	28.371127531062186	23.920504788597295	25.82170531017221
115-119	21.686611396631278	28.695652173913043	23.800877818209102	25.81685861124658
120-124	21.980465815176558	28.45304282494365	23.87678437265214	25.689706987227652
125-129	24.072934005213913	24.931025833960373	26.033092398780887	24.962947762044827
130-134	23.71227748491099	28.95255558102223	25.68569027427611	21.649476659790665
135-139	25.429180450610822	22.007960944005987	26.146582218521036	26.416276386862155
140-144	24.3861257544503	25.144014057605624	25.06914202765681	25.400718160287266
145-149	24.344869737947896	25.064558025823207	25.43204217281687	25.158530063412027
150-154	24.50702503648129	24.915004316634707	25.41160830933081	25.166362337553196
155-159	24.224157689663077	24.603101841240736	25.730766292306516	25.44197417678967
160-164	23.777361806165718	24.5933453031287	25.916644382473166	25.712648508232416
165-169	23.99810509103968	24.476416788282126	25.639340755060097	25.8861373656181
170-174	23.89853953794068	24.672714350128775	25.77551223165634	25.653233880274207
175-179	24.075433044382272	24.263480560779097	25.513308566099468	26.147777828739166
180-184	24.01241837062413	24.114885221833084	25.607536666309816	26.265159741232967
185-189	23.75500026770076	23.953863686774817	26.52685038587152	25.76428565965291
190-194	23.731900694890868	23.966847277068602	26.27881348149509	26.022438546545445
195-199	23.963024231841437	23.985234200287962	26.592990840302665	25.45875072756793
200-204	24.238705223594383	23.479328066272913	26.342716882718413	25.939249827414283
205-209	24.205879185958867	23.370610223199865	26.343275546462795	26.08023504437847
210-214	24.721331736896992	23.639874859989956	25.92329380865938	25.71549959445367
215-219	24.47944619453195	24.076537160191343	25.898961614747407	25.545055030529305
220-224	24.651100691908365	24.26312937351271	26.18256642241752	24.903203512161408
225-229	24.029747244075658	25.135305323736418	26.056736571096074	24.77821086109185
230-234	24.537791835137604	25.313029868266597	25.729587844006787	24.41959045258902
235-239	24.687381743261994	25.11289576588319	26.18172644325779	24.017996047597023
240-244	24.377344566754815	24.786737768719195	26.166023878341694	24.669893786184296
245-249	23.889682766504716	25.732113937315425	26.106506620939317	24.271696675240545
250-252	22.958505353019383	26.209968978144754	26.018706411960906	24.812819256874956
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.3333333333333333
19	1.0
20	0.6666666666666666
21	3.0
22	3.666666666666667
23	2.1666666666666665
24	4.166666666666667
25	5.666666666666665
26	15.166666666666675
27	18.500000000000004
28	23.33333333333332
29	31.833333333333293
30	44.6666666666667
31	62.33333333333349
32	79.16666666666664
33	84.99999999999989
34	106.33333333333293
35	154.6666666666666
36	197.66666666666782
37	237.1666666666689
38	263.0000000000028
39	324.6666666666661
40	399.3333333333287
41	438.6666666666599
42	495.1666666666567
43	554.3333333333248
44	614.4999999999977
45	639.6666666666665
46	630.333333333332
47	618.4999999999976
48	574.8333333333262
49	555.1666666666572
50	518.333333333323
51	510.66666666665645
52	450.9999999999932
53	446.4999999999934
54	443.83333333332666
55	441.3333333333267
56	408.83333333332854
57	374.33333333333053
58	368.49999999999744
59	347.83333333333184
60	350.499999999999
61	329.1666666666669
62	290.3333333333352
63	270.33333333333616
64	235.50000000000185
65	179.33333333333377
66	147.99999999999963
67	115.3333333333329
68	105.83333333333303
69	78.6666666666667
70	62.333333333333464
71	47.16666666666668
72	34.3333333333333
73	21.83333333333334
74	12.833333333333334
75	12.333333333333332
76	4.666666666666667
77	2.6666666666666665
78	1.3333333333333333
79	1.5
80	1.1666666666666665
81	0.8333333333333333
82	0.8333333333333333
83	0.3333333333333333
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014944890715486644
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0014944890715486642
15-19	0.0
20-24	0.0
25-29	0.0029889781430973285
30-34	0.0029889781430973285
35-39	0.003736222678871661
40-44	0.007472445357743322
45-49	0.003736222678871661
50-54	7.472445357743321E-4
55-59	7.472445357743321E-4
60-64	7.472445357743321E-4
65-69	0.0
70-74	0.0014944890715486642
75-79	0.0
80-84	7.474288447739775E-4
85-89	7.475461796652488E-4
90-94	0.0029909449142720415
95-99	0.0022441819582731766
100-104	0.001496613911026303
105-109	0.0
110-114	0.0
115-119	7.502663445523161E-4
120-124	0.0
125-129	0.0
130-134	0.0
135-139	7.640003056001222E-4
140-144	0.0
145-149	0.0
150-154	7.640003056001222E-4
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0015288182235132244
180-184	0.003058618422058756
185-189	7.648534540781986E-4
190-194	0.0
195-199	0.0
200-204	0.0
205-209	7.691183596243626E-4
210-214	0.0030897813207270254
215-219	0.010110594347400022
220-224	0.007853053659915659
225-229	0.0119548584544759
230-234	0.020375396301458064
235-239	0.05295096572475584
240-244	0.019323841228293618
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	5.0
80-84	4.0
85-89	5.0
90-94	11.0
95-99	9.0
100-104	11.0
105-109	19.0
110-114	27.0
115-119	37.0
120-124	63.0
125-129	396.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	1.0
160-164	0.0
165-169	5.0
170-174	5.0
175-179	8.0
180-184	7.0
185-189	11.0
190-194	18.0
195-199	19.0
200-204	77.0
205-209	75.0
210-214	155.0
215-219	214.0
220-224	317.0
225-229	483.0
230-234	654.0
235-239	907.0
240-244	1256.0
245-249	3586.0
250-253	18380.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.81318886605641
#Duplication Level	Percentage of total
1	99.63011395479171
2	0.3586773771716794
3	0.011208668036614982
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	7.472445357743321E-4	0.0	0.0
20-24	0.0	0.0	0.0	0.014197646179712309	0.0	0.0
25-29	0.0	0.0	0.0	0.12254810386699048	0.0	0.0
30-34	0.0	0.0	0.0	0.9938352325798616	0.006725200821968989	0.0
35-39	0.0	0.0	0.0	2.8910891089108914	0.0074724453577433205	0.0
40-44	0.0	0.0	0.0	6.248458808144965	0.008966934429291985	0.0
45-49	0.0	0.0	0.0	9.846441247898374	0.01793386885858397	0.0
50-54	0.0	0.0	0.0	13.406314216327294	0.0418456940033626	0.0
55-59	0.0	0.0	0.0	16.623201942835795	0.06949374182701289	0.0
60-64	0.0	0.0	0.0	19.697365963011396	0.09340556697179152	0.0
65-69	0.0	0.0	0.0	22.782738651223614	0.12180085933121614	0.0
70-74	0.0	0.0	0.0	25.877825518400897	0.14048197272557444	0.0
75-79	0.0	0.0	0.0	28.79282645245657	0.15467961890528675	0.0
80-84	0.0	0.0	0.0	31.4963571828881	0.1763497104427424	0.0
85-89	0.0	0.0	0.0	34.02652718101999	0.19353633476555201	0.0
90-94	0.0	0.0	0.0	36.51559872968429	0.211470203624136	0.0
95-99	0.0	0.0	0.0	39.03605454885111	0.31533719409676814	0.0
100-104	0.0	0.0	0.0	41.33682047450028	0.5185877078273865	0.0
105-109	0.0	0.0	0.0	43.48066504763684	0.8436390808892209	0.0
110-114	0.0	0.0	0.0	45.60956473005791	1.1081636465533347	0.0
115-119	0.0	0.0	0.0	46.89183635344667	1.2680739772090415	0.0
120-124	0.0	0.0	0.0	46.91574817859144	1.2882495796749487	0.0
125-129	0.0	0.0	0.0	46.91574817859144	1.288996824210723	0.0
130-134	0.0014944890715486642	0.0	0.0	46.91574817859144	1.288996824210723	0.0
135-139	0.0037362226788716603	0.0	0.0	46.92471511302073	1.288996824210723	0.0
140-144	0.0037362226788716603	0.0	0.0	46.947132449093964	1.288996824210723	0.0
145-149	0.0037362226788716603	0.0	0.0	46.95908836166636	1.288996824210723	0.0
150-154	0.0037362226788716603	0.0	0.0	46.97552774145339	1.288996824210723	0.0
155-159	0.0037362226788716603	0.0	0.0	46.98598916495423	1.288996824210723	0.0
160-164	0.0037362226788716603	0.0	0.0	47.00242854474127	1.288996824210723	0.0
165-169	0.0037362226788716603	0.0	0.0	47.0188679245283	1.288996824210723	0.0
170-174	0.0037362226788716603	0.0	0.0	47.037549037922666	1.288996824210723	0.0
175-179	0.0037362226788716603	0.0	0.0	47.077900242854476	1.288996824210723	0.0
180-184	0.0037362226788716603	0.0	0.0	47.10779002428545	1.2904913132822717	0.0
185-189	0.0037362226788716603	0.0	0.0	47.14888847375304	1.2927330468895946	0.0
190-194	0.0037362226788716603	0.0	0.0	47.183261722398655	1.2927330468895946	0.0
195-199	0.0037362226788716603	0.0	0.0	47.22211843825892	1.2927330468895946	0.0
200-204	0.0037362226788716603	0.0	0.0	47.24677750793948	1.3076779376050816	0.0
205-209	0.0037362226788716603	0.0	0.0	47.281898001120865	1.3338314963571831	0.0
210-214	0.0037362226788716603	0.0	0.0	47.317765738838034	1.3577433215019614	0.0
215-219	0.0037362226788716603	0.0	0.0	47.34616103119746	1.3891275920044834	0.0
220-224	0.0037362226788716603	0.0	0.0	47.38427050252195	1.4414347095086868	0.0
225-229	0.0037362226788716603	0.0	0.0	47.40594059405941	1.4944890715486643	0.0
230-234	0.0037362226788716603	0.0	0.0	47.42462170745376	1.5385764991593498	0.0
235-239	0.0037362226788716603	0.0	0.0	47.44928077713432	1.5647300579114516	0.0
240-241	0.0037362226788716607	0.0	0.0	47.511675695871475	1.565477302447226	0.0
>>END_MODULE
