##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665119
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1341551
Total Bases	332 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.191145919909122	25.0	18.0	32.0	18.0	33.0
2	27.059507987396678	29.0	25.0	31.0	18.0	33.0
3	28.68470225880343	30.0	27.0	33.0	18.0	33.0
4	30.67832307530612	31.0	30.0	33.0	27.0	33.0
5	30.659295844884017	33.0	31.0	33.0	28.0	33.0
6	34.723855447910665	37.0	34.0	38.0	29.0	38.0
7	35.11965255141251	37.0	35.0	38.0	29.0	38.0
8	36.094729905907414	38.0	37.0	38.0	33.0	38.0
9	36.43989978763386	38.0	37.0	38.0	34.0	38.0
10-14	36.66347742277408	38.0	38.0	38.0	34.0	38.0
15-19	36.72646556113036	38.0	38.0	38.0	34.0	38.0
20-24	36.75023118763282	38.0	38.0	38.0	34.0	38.0
25-29	36.696672284542295	38.0	38.0	38.0	34.0	38.0
30-34	36.67182760849196	38.0	38.0	38.0	34.0	38.0
35-39	36.64648425590976	38.0	38.0	38.0	34.0	38.0
40-44	36.60805962650693	38.0	38.0	38.0	34.0	38.0
45-49	36.58859633364665	38.0	38.0	38.0	34.0	38.0
50-54	36.538449600499725	38.0	38.0	38.0	34.0	38.0
55-59	36.45878419828989	38.0	38.0	38.0	34.0	38.0
60-64	36.404924747549664	38.0	38.0	38.0	34.0	38.0
65-69	36.332704906485105	38.0	37.2	38.0	33.2	38.0
70-74	36.222650797472475	38.0	37.0	38.0	33.0	38.0
75-79	36.06237366209017	38.0	37.0	38.0	32.8	38.0
80-84	35.85664944115624	38.0	37.0	38.0	31.2	38.0
85-89	35.6857425987175	38.0	36.4	38.0	31.0	38.0
90-94	35.56266979263415	38.0	36.0	38.0	29.2	38.0
95-99	35.414123595602085	38.0	36.0	38.0	29.0	38.0
100-104	35.259914202863136	38.0	35.2	38.0	28.0	38.0
105-109	35.03793221108851	38.0	35.0	38.0	27.6	38.0
110-114	34.85313027953043	38.0	34.8	38.0	27.0	38.0
115-119	34.68580287422674	38.0	34.0	38.0	26.2	38.0
120-124	34.83570695999585	38.0	34.0	38.0	28.2	38.0
125-129	33.804961441230276	34.2	33.2	35.6	31.0	37.0
130-134	35.35990268062997	36.2	36.0	37.2	33.0	38.0
135-139	36.53116063692319	38.0	38.0	38.0	34.0	38.0
140-144	36.4794110768991	38.0	38.0	38.0	34.0	38.0
145-149	36.46281311742784	38.0	38.0	38.0	34.0	38.0
150-154	36.45037446670724	38.0	38.0	38.0	34.0	38.0
155-159	36.443899100860165	38.0	38.0	38.0	34.0	38.0
160-164	36.43328887793009	38.0	38.0	38.0	34.0	38.0
165-169	36.38562050821243	38.0	38.0	38.0	33.6	38.0
170-174	36.35220755705602	38.0	38.0	38.0	33.0	38.0
175-179	36.290298933497766	38.0	38.0	38.0	33.0	38.0
180-184	36.20840716899974	38.0	37.4	38.0	33.0	38.0
185-189	36.10035001247022	38.0	37.0	38.0	33.0	38.0
190-194	35.96980308341993	38.0	37.0	38.0	31.4	38.0
195-199	35.81263941983426	38.0	37.0	38.0	31.0	38.0
200-204	35.601704830195516	38.0	37.0	38.0	29.0	38.0
205-209	35.40917276908399	38.0	36.2	38.0	28.6	38.0
210-214	35.222113364366706	38.0	36.0	38.0	27.6	38.0
215-219	34.977144319767284	38.0	35.2	38.0	26.0	38.0
220-224	34.78850152886433	38.0	35.0	38.0	24.8	38.0
225-229	34.55805266311336	38.0	35.0	38.0	23.4	38.0
230-234	34.32878917234568	38.0	34.0	38.0	23.0	38.0
235-239	34.12685055423059	38.0	34.0	38.0	23.0	38.0
240-244	34.01413617498342	38.0	33.2	38.0	23.0	38.0
245-249	34.29023265539602	38.0	33.2	38.0	24.4	38.0
250-252	35.60316226120523	37.666666666666664	34.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	4.0
18	9.0
19	19.0
20	65.0
21	291.0
22	793.0
23	1800.0
24	3439.0
25	5786.0
26	8884.0
27	12474.0
28	16977.0
29	22462.0
30	29241.0
31	37959.0
32	48729.0
33	66023.0
34	95791.0
35	151922.0
36	287649.0
37	551232.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.39455401875626	26.34965800436806	11.48968122413036	25.766106752745323
2	16.309380140986487	20.255197917031605	34.65156271081073	28.783859231171178
3	18.352021956740984	26.533472178015383	30.361068229439596	24.753437635804037
4	15.533226491744623	18.521560881070403	36.579851664119865	29.36536096306511
5	16.55065588703149	32.70877840574503	34.00154449133907	16.73902121588441
6	29.92147876930233	36.74628116176238	18.892130583475208	14.44010948546008
7	24.98347062467249	29.832410396623015	25.27865135205445	19.90546762665005
8	24.89581089351057	33.44658533294672	21.036397423579125	20.621206349963586
9	24.133558843458058	15.563702013564896	20.58736492313747	39.715374219839575
10-14	24.903916623780734	22.301823750439045	27.674993991993695	25.119265633786526
15-19	24.410447310612867	26.85673522661457	24.8450487532714	23.887768709501167
20-24	24.29052599000768	25.80537053328306	25.61593297067825	24.28817050603101
25-29	24.413034461928007	25.697779574735296	25.48499217458794	24.40419378874876
30-34	24.241659846055448	25.536085133154728	25.797758068820343	24.42449695196948
35-39	23.946152948204507	25.053137557134892	26.31096618741363	24.68974330724697
40-44	23.361223031708317	24.648687606751974	26.972973263103654	25.017116098436055
45-49	22.47540462777197	24.688238962552298	27.621957101204163	25.21439930847157
50-54	21.9510420682563	24.91091605222521	27.26667996478087	25.871361914737623
55-59	21.541628253399946	24.677716170782702	26.875919085857674	26.904736489959674
60-64	21.844132258338153	24.790112214958487	25.898158712212187	27.467596814491174
65-69	22.050850098132685	25.136576991854948	25.02876148577281	27.78381142423956
70-74	22.23091371435463	25.6556150542607	24.521651133047065	27.5918200983376
75-79	22.644341735968055	25.71817167780468	24.285987747139163	27.3514988390881
80-84	22.570933680333578	25.654799958016465	24.420177022604314	27.35408933904564
85-89	22.525108523807848	24.93094075038421	25.030086693809295	27.513864031998647
90-94	22.551805742221198	24.072482563432608	25.541660044115883	27.834051650230318
95-99	22.26199420502314	23.90443611099887	26.135485801591003	27.698083882386985
100-104	21.970113565944413	24.713073175212195	26.177841847449105	27.13897141139429
105-109	21.509546730672906	26.678698654476214	25.543926025792075	26.267828589058805
110-114	21.130121714593372	28.27493687574851	24.95261020457776	25.642331205080364
115-119	21.03244744908927	28.680801372765792	24.888092826081476	25.398658352063467
120-124	21.28940210335559	28.634135029483865	24.81916919901416	25.25729366814638
125-129	23.761283868071278	25.359831532959355	26.297900254805352	24.58098434416402
130-134	23.144113902835517	29.28319922382586	26.320947167510063	21.251739705828562
135-139	25.079582899409463	22.205574003514165	26.84083285200077	25.874010245075603
140-144	24.026666814575783	25.60348793683721	25.42161968578407	24.948225562802936
145-149	23.953924779604794	25.583952854434227	26.056703720004844	24.405418645956132
150-154	23.918170348819082	25.40155988784446	26.140593781555737	24.53967598178072
155-159	23.695067856141684	25.114087963386318	26.306746038289663	24.88409814218234
160-164	23.506023278206207	25.182293380472792	26.481901532717018	24.829781808603983
165-169	23.389936360196607	24.992789327845426	26.371841620659282	25.24543269129868
170-174	23.357567611447223	25.14934610623755	26.415353150003924	25.0777331323113
175-179	23.51063797890394	24.720573155593932	26.31333165428368	25.45545721121845
180-184	23.641418745090043	24.466231492087097	26.39343819901291	25.498911563809944
185-189	23.378242502918525	24.0646696840015	27.25149344879692	25.305594364283056
190-194	23.459084787753177	24.265616705007965	26.90746200612461	25.367836501114247
195-199	23.290143598579842	24.29918753533784	27.314138151245732	25.09653071483659
200-204	23.814084329445954	23.728325825273615	26.929446254951795	25.52814359032864
205-209	24.000352309339622	23.567781371012398	27.01010213964797	25.42176418000001
210-214	24.121120499849173	24.11567831149247	26.42761339236625	25.33558779629211
215-219	23.965501391528015	24.579151139086967	26.40047062864057	25.05487684074445
220-224	24.300681083398633	24.509806171783705	26.923336459422124	24.266176285395535
225-229	23.763513356427126	25.41169723881927	26.626585760893995	24.19820364385961
230-234	23.991383685291765	25.715485590562327	26.560765185479028	23.732365538666883
235-239	24.33317358056841	25.381359429692417	26.682028596941166	23.603438392798008
240-244	24.144078668637572	25.328523239494615	26.48151937381612	24.045878718051693
245-249	23.688770580912937	25.969395144362007	26.60305168579699	23.73878258892806
250-252	22.584874631696017	26.831153817096332	26.57094716276357	24.013024388444084
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.3333333333333333
9	0.0
10	0.0
11	0.3333333333333333
12	0.6666666666666666
13	2.1666666666666665
14	2.5
15	5.666666666666665
16	10.0
17	16.333333333333343
18	27.833333333333304
19	39.166666666666664
20	65.16666666666683
21	95.83333333333307
22	127.16666666666596
23	181.00000000000074
24	293.50000000000085
25	422.4999999999937
26	597.1666666666633
27	816.8333333333546
28	1138.6666666666897
29	1513.666666666606
30	1993.999999999831
31	2645.8333333334076
32	3375.0000000004
33	4430.000000000488
34	5706.333333332676
35	7160.499999998034
36	8958.166666664898
37	10933.333333335113
38	13262.333333339311
39	16028.833333344312
40	18866.833333336897
41	22238.499999991407
42	25468.166666646433
43	28785.166666634606
44	31878.666666623452
45	34293.16666662766
46	35977.499999972984
47	36793.999999978856
48	36810.333333312214
49	35808.49999997162
50	33969.33333329195
51	31313.499999959036
52	28517.99999996911
53	25600.33333331283
54	22851.333333322724
55	20273.333333331942
56	18112.000000006323
57	16124.666666677771
58	14500.166666674852
59	13065.500000005622
60	11718.50000000321
61	10452.83333333427
62	9078.166666665105
63	7752.66666666417
64	6592.999999998551
65	5456.666666666245
66	4360.500000000565
67	3437.833333333751
68	2638.8333333333944
69	1932.333333333181
70	1354.33333333331
71	932.6666666667003
72	633.0000000000001
73	385.499999999996
74	259.0000000000028
75	142.49999999999952
76	83.3333333333333
77	53.50000000000007
78	32.49999999999996
79	17.00000000000001
80	5.833333333333332
81	1.6666666666666665
82	0.6666666666666666
83	0.8333333333333333
84	0.8333333333333333
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03369234565066852
2	2.9816235089087184E-4
3	2.9816235089087184E-4
4	7.454058772271796E-5
5	6.708652895044616E-4
6	2.2362176316815388E-4
7	0.0
8	0.0
9	0.0
10-14	5.814165842372E-4
15-19	0.0
20-24	5.963247017817437E-5
25-29	0.0018038822228897747
30-34	0.002161677043958821
35-39	0.002444931277305149
40-44	0.009228124760072483
45-49	0.004382986558095815
50-54	5.516003491481128E-4
55-59	4.472435263363078E-5
60-64	5.963247017817437E-5
65-69	0.0
70-74	6.857734070490053E-4
75-79	6.261460709334049E-4
80-84	0.0024748201953676734
85-89	0.001878519428512645
90-94	0.004949894248193065
95-99	0.002206652729964898
100-104	0.0014910489350332087
105-109	9.990933600553527E-4
110-114	4.026811405182685E-4
115-119	6.861991770681782E-4
120-124	6.565854928032261E-4
125-129	0.0011377114365597881
130-134	0.0022785804443831493
135-139	0.0015440380642859498
140-144	8.544676666436809E-4
145-149	0.0011392902221915747
150-154	0.0012592155087380562
155-159	0.0013491594736479175
160-164	0.001574022217398552
165-169	0.001574030475928352
170-174	0.0021886676485689334
175-179	0.0026684184283974886
180-184	0.0033281651825348684
185-189	0.002533873313231272
190-194	0.0017095826908651596
195-199	0.0010500919355489573
200-204	7.505501532623413E-4
205-209	0.001187376593620962
210-214	0.003241087461725018
215-219	0.011558070354368618
220-224	0.012851576681915675
225-229	0.013978315324758727
230-234	0.027187691287088728
235-239	0.06841630039140906
240-244	0.028689314248997785
245-249	9.166415354112375E-5
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	29.0
80-84	29.0
85-89	30.0
90-94	44.0
95-99	56.0
100-104	87.0
105-109	167.0
110-114	250.0
115-119	361.0
120-124	916.0
125-129	5418.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	6.0
165-169	9.0
170-174	13.0
175-179	38.0
180-184	95.0
185-189	209.0
190-194	355.0
195-199	628.0
200-204	1286.0
205-209	2565.0
210-214	6517.0
215-219	10037.0
220-224	14037.0
225-229	21618.0
230-234	34256.0
235-239	41495.0
240-244	63110.0
245-249	172958.0
250-253	964932.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.15201911039611
#Duplication Level	Percentage of total
1	96.39821261332266
2	3.348255214787617
3	0.2089238785509646
4	0.029056535131021157
5	0.010432307396571751
6	0.00240336147295002
7	0.0
8	0.0
9	0.0013349149142301237
>10	0.0013811744239641437
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	1.4908117544543592E-4	0.0	0.0	0.0	0.0
5	0.0	1.4908117544543592E-4	0.0	0.0	0.0	0.0
6	0.0	1.4908117544543592E-4	0.0	0.0	0.0	0.0
7	0.0	1.4908117544543592E-4	0.0	0.0	0.0	0.0
8	0.0	1.4908117544543592E-4	0.0	0.0	0.0	0.0
9	0.0	1.4908117544543592E-4	0.0	7.454058772271796E-5	0.0	0.0
10-14	5.963247017817437E-5	2.236217631681539E-4	0.0	1.4908117544543592E-4	2.9816235089087185E-5	0.0
15-19	7.454058772271796E-5	2.9816235089087184E-4	1.0435682281180513E-4	4.0251917370267697E-4	1.4908117544543592E-4	0.0
20-24	7.454058772271796E-5	2.9816235089087184E-4	1.4908117544543592E-4	0.004114640442294032	1.4908117544543592E-4	0.0
25-29	7.454058772271796E-5	2.9816235089087184E-4	2.087136456236103E-4	0.12402062985305815	1.9380552807906671E-4	0.0
30-34	7.454058772271796E-5	3.727029386135898E-4	3.4288670352450263E-4	1.2154140990540054	2.8325423334632824E-4	0.0
35-39	7.454058772271796E-5	3.727029386135898E-4	4.919678789699386E-4	3.3943249268943183	2.9816235089087184E-4	0.0
40-44	7.454058772271796E-5	3.727029386135898E-4	5.217841140590258E-4	7.143448143231231	3.1307046843541544E-4	0.0
45-49	7.454058772271796E-5	4.472435263363077E-4	5.665084666926565E-4	11.088001872459563	5.217841140590258E-4	5.963247017817437E-5
50-54	7.454058772271796E-5	4.472435263363077E-4	5.963247017817437E-4	14.99315344701767	9.690276403953334E-4	7.454058772271796E-5
55-59	1.4908117544543592E-4	4.472435263363077E-4	5.963247017817437E-4	18.706989149126645	0.0016398929298997952	1.0435682281180513E-4
60-64	1.4908117544543592E-4	4.7705976142539496E-4	5.963247017817437E-4	22.3258005100067	0.0023852988071269747	1.4908117544543592E-4
65-69	1.4908117544543592E-4	5.217841140590258E-4	5.963247017817437E-4	25.75700811970622	0.0030561640966314365	1.4908117544543592E-4
70-74	1.4908117544543592E-4	5.217841140590258E-4	5.963247017817437E-4	29.113168265686504	0.0034288670352450256	1.4908117544543592E-4
75-79	1.4908117544543592E-4	5.217841140590258E-4	5.963247017817437E-4	32.35390976563694	0.003741937503680441	1.4908117544543592E-4
80-84	1.4908117544543592E-4	5.217841140590258E-4	5.963247017817437E-4	35.472419609839655	0.004233905382650381	1.4908117544543592E-4
85-89	1.4908117544543592E-4	5.217841140590258E-4	5.963247017817437E-4	38.47961054033726	0.004591700203719426	1.4908117544543592E-4
90-94	1.4908117544543592E-4	5.217841140590258E-4	5.963247017817437E-4	41.34990022742333	0.011926494035634875	1.4908117544543592E-4
95-99	1.4908117544543592E-4	5.217841140590258E-4	5.963247017817437E-4	44.093321834205334	0.12847815699887669	1.4908117544543592E-4
100-104	1.4908117544543592E-4	5.217841140590258E-4	5.963247017817437E-4	46.7156746184081	0.37462608577683587	1.4908117544543592E-4
105-109	1.4908117544543592E-4	5.217841140590258E-4	5.963247017817437E-4	49.21190472818402	0.777011086421612	1.4908117544543592E-4
110-114	1.4908117544543592E-4	5.217841140590258E-4	5.963247017817437E-4	51.57454319664329	1.1162303930301571	1.4908117544543592E-4
115-119	2.087136456236103E-4	5.217841140590258E-4	5.963247017817437E-4	52.91481278013284	1.3338143685927706	1.4908117544543592E-4
120-124	2.2362176316815385E-4	5.963247017817437E-4	5.963247017817437E-4	52.94139395371477	1.3589941791255047	1.7889741053452312E-4
125-129	2.2362176316815385E-4	5.963247017817437E-4	5.963247017817437E-4	52.94222880829726	1.3590239953605938	2.2362176316815385E-4
130-134	2.683461158017846E-4	5.963247017817437E-4	5.963247017817437E-4	52.944062506755245	1.3590836278307719	2.2362176316815385E-4
135-139	3.876110561581334E-4	5.963247017817437E-4	5.963247017817437E-4	52.94707394649923	1.3590985359483165	2.2362176316815385E-4
140-144	4.472435263363077E-4	5.963247017817437E-4	5.963247017817437E-4	52.95174018729068	1.3590985359483165	2.2362176316815385E-4
145-149	4.472435263363077E-4	5.963247017817437E-4	5.963247017817437E-4	52.95861282947872	1.3592028927711284	2.2362176316815385E-4
150-154	4.472435263363077E-4	5.963247017817437E-4	5.963247017817437E-4	52.96784095423878	1.3593519739465738	2.2362176316815385E-4
155-159	4.472435263363077E-4	5.963247017817437E-4	5.963247017817437E-4	52.98227201202191	1.3594563307693854	2.2362176316815385E-4
160-164	4.472435263363077E-4	5.963247017817437E-4	5.963247017817437E-4	52.99965487707884	1.3595010551220192	2.8325423334632824E-4
165-169	4.472435263363077E-4	6.559571719599181E-4	5.963247017817437E-4	53.01811112659899	1.3596799525325536	2.9816235089087184E-4
170-174	4.472435263363077E-4	6.708652895044616E-4	5.963247017817437E-4	53.03531509424539	1.359769401237821	2.9816235089087184E-4
175-179	4.472435263363077E-4	6.708652895044616E-4	6.410490544153745E-4	53.0555006854007	1.3599482986483555	2.9816235089087184E-4
180-184	4.472435263363077E-4	6.708652895044616E-4	6.708652895044616E-4	53.08108301510714	1.3602762772343353	2.9816235089087184E-4
185-189	4.472435263363077E-4	6.708652895044616E-4	6.708652895044616E-4	53.111137780076945	1.360678796408038	2.9816235089087184E-4
190-194	4.472435263363077E-4	6.708652895044616E-4	6.708652895044616E-4	53.1398060901151	1.3611111318168299	3.27978585979959E-4
195-199	4.472435263363077E-4	7.155896421380924E-4	6.708652895044616E-4	53.16778862674621	1.3634517062713232	3.727029386135898E-4
200-204	4.472435263363077E-4	7.454058772271796E-4	6.708652895044616E-4	53.19517483867553	1.3761683305368189	3.727029386135898E-4
205-209	4.472435263363077E-4	7.454058772271796E-4	6.708652895044616E-4	53.219817956976655	1.4082058751400433	4.0251917370267697E-4
210-214	5.217841140590258E-4	7.454058772271796E-4	6.708652895044616E-4	53.24371566940056	1.4462215748786293	4.472435263363077E-4
215-219	5.217841140590258E-4	7.454058772271796E-4	6.708652895044616E-4	53.274620197070405	1.4923771067965363	4.472435263363077E-4
220-224	5.217841140590258E-4	7.454058772271796E-4	6.708652895044616E-4	53.3080889209579	1.54941556452196	4.472435263363077E-4
225-229	5.217841140590258E-4	7.454058772271796E-4	6.708652895044616E-4	53.33498316500826	1.607333601182512	4.472435263363077E-4
230-234	5.217841140590258E-4	7.454058772271796E-4	6.708652895044616E-4	53.3606996677726	1.6527884515758253	5.217841140590258E-4
235-239	5.217841140590258E-4	7.901302298608103E-4	6.708652895044616E-4	53.39586791706017	1.682977389603526	5.217841140590258E-4
240-241	5.217841140590258E-4	8.199464649498976E-4	6.708652895044616E-4	53.465168301465994	1.6842818498886736	5.217841140590258E-4
>>END_MODULE
