FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR5665121

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5665121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length75-252
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT1382.8354222313540167No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT180.36983768235052394RNA PCR Primer, Index 33 (95% over 22bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT110.22601191699198686RNA PCR Primer, Index 33 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT110.22601191699198686RNA PCR Primer, Index 33 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGT80.16437230326689953No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG70.14382576535853708RNA PCR Primer, Index 33 (96% over 29bp)
TCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG70.14382576535853708No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGT70.14382576535853708No Hit
GTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT60.12327922745017465No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC60.12327922745017465No Hit
GGGATAATCGCGACCAAAGTGCTGACAGTCGTTTCTGGGGAATGGTTCCT50.10273268954181221No Hit
GCCGTATCAGGTGTGGAAGACGAACCATTTGACGACGACACTGAAATAGC50.10273268954181221No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGT50.10273268954181221No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)