FastQCFastQC Report
Tue 12 Sep 2023
stdin:SRR5665124

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5665124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960616
Total Bases485.9 Mbp
Sequences flagged as poor quality0
Sequence length75-252
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTATATTAAACGATTGCGTAAACATATAAAAACAGCTTTAGGTTCTT83060.4236423654606512No Hit
GTCTAGTGTAATCAATATTAATGAAGTTCTCAATACCAACCTTGAAGCTG67150.3424943997192719No Hit
GATTCATCGTCCTTGCGTCATATATTCTTTACACAAGTACAGGGTACAGT64720.33010033581282616No Hit
GTATTCATAAGCGTGCTCAAAAAGCGTCCATGCGCGTGAAGACCGCCGGA55430.28271726845032374No Hit
GAATCCCCAGTTGACTGTTACGGCTGTTACTGATTTCCCCCAACCTGCAT51760.2639986616451156No Hit
CCTCCTTTGACTTGTCTAGTAAAAAACTTTTTACGGCAAAAGTTGGAGAG48500.24737123434675634No Hit
GCTATTGGTGGTATCTTCGGTACATTGCAACAGTCTAGCGCTAATCGTGC44190.2253883473357353No Hit
GAGCATGCTCTCTCCTAGAAATGAACATCATTTCCTTGGTGCTGCTATTG40530.20672074490874298No Hit
GGAAACAGGTCGTATTTCTGCTGAGATAGACCAATCTATGGAGCGTCAGA36620.18677803302635498No Hit
GAATGAGGCTTGTAATGGCGCCCCTAATTCTCAACATCTGAGAGGCGAAG29520.15056492449311848No Hit
GCTGCATATACCCGTATTAAAGAGTATTATGACTTTTACGCTGTGCCGTT25780.13148928704039956No Hit
GCTCAAAAAGCGTCCATGCGCGTGAAGACCGCCGGAAGTGCTTACGAGTA24430.12460369598126303No Hit
AAGTTTATCAGGAGATCATGTTTTTGGTTATGAGAAGCATTCGTATATCC23320.11894220999930634No Hit
GCCTTACGGATCTACTAGCTTTAATGCGTTGCTTGAGCTGATCCATGATT22350.11399478531237121No Hit
GGATAATTGGTTTCTTTCTATGGATGCTATCCGCTCCTTTATTGCTAAGA22320.11384177217772375No Hit
CGGTTATTCCTTTATGTCAGTAAGTTCCTTAGGAATTGGCATCTGCCTCC21560.10996543943332096No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)