##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665126
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2407660
Total Bases	600.9 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.11649070051419	32.0	25.0	33.0	18.0	33.0
2	28.277442412965286	30.0	27.0	33.0	18.0	33.0
3	30.51269822150968	31.0	29.0	33.0	27.0	33.0
4	30.912483074852762	33.0	31.0	33.0	28.0	33.0
5	31.92365533339425	33.0	32.0	33.0	30.0	34.0
6	35.61531819276808	37.0	36.0	38.0	30.0	38.0
7	36.090141880498074	38.0	36.0	38.0	33.0	38.0
8	36.49921334407682	38.0	37.0	38.0	34.0	38.0
9	36.685395778473705	38.0	38.0	38.0	34.0	38.0
10-14	36.79125158867946	38.0	38.0	38.0	34.8	38.0
15-19	36.7408369121886	38.0	38.0	38.0	34.0	38.0
20-24	36.73028334565512	38.0	38.0	38.0	34.0	38.0
25-29	36.68337580887667	38.0	38.0	38.0	34.0	38.0
30-34	36.65623094622995	38.0	38.0	38.0	34.0	38.0
35-39	36.6051955840941	38.0	38.0	38.0	34.0	38.0
40-44	36.61961730476894	38.0	38.0	38.0	34.0	38.0
45-49	36.566750704003056	38.0	38.0	38.0	34.0	38.0
50-54	36.45146399408554	38.0	38.0	38.0	33.8	38.0
55-59	36.34812515056113	38.0	37.6	38.0	33.2	38.0
60-64	36.32910801358996	38.0	37.2	38.0	33.0	38.0
65-69	36.26523786581161	38.0	37.0	38.0	33.0	38.0
70-74	36.18410822126047	38.0	37.0	38.0	33.0	38.0
75-79	36.10477131417048	38.0	37.0	38.0	33.0	38.0
80-84	36.04081243887	38.0	37.0	38.0	32.8	38.0
85-89	35.93341021428734	38.0	37.0	38.0	31.4	38.0
90-94	35.853006731186866	38.0	37.0	38.0	31.0	38.0
95-99	35.75951992441367	38.0	36.4	38.0	31.0	38.0
100-104	35.6539030880765	38.0	36.0	38.0	31.0	38.0
105-109	35.51331668139312	38.0	36.0	38.0	30.6	38.0
110-114	35.43146196694157	38.0	35.2	38.0	29.8	38.0
115-119	35.31648803876443	38.0	35.0	38.0	29.0	38.0
120-124	35.41255707827655	38.0	35.0	38.0	30.6	38.0
125-129	32.857283735388066	34.4	32.8	35.4	28.4	35.6
130-134	33.76280076067535	36.0	34.4	36.4	27.2	37.2
135-139	34.96902379441783	38.0	36.0	38.0	27.2	38.0
140-144	34.82844106514532	38.0	35.2	38.0	26.2	38.0
145-149	34.790162842474594	38.0	35.0	38.0	26.0	38.0
150-154	34.75666806545671	38.0	35.0	38.0	25.2	38.0
155-159	34.743394477778	38.0	35.0	38.0	25.0	38.0
160-164	34.6883280133542	38.0	35.0	38.0	25.0	38.0
165-169	34.64221216386214	38.0	35.0	38.0	25.0	38.0
170-174	34.579628493650794	38.0	35.0	38.0	25.0	38.0
175-179	34.51843078621867	38.0	35.0	38.0	25.0	38.0
180-184	34.39811667667344	38.0	34.2	38.0	24.8	38.0
185-189	34.30313782653542	38.0	34.0	38.0	24.0	38.0
190-194	34.233146189775006	38.0	34.0	38.0	24.0	38.0
195-199	34.13155436186952	38.0	34.0	38.0	23.0	38.0
200-204	34.01891516441242	38.0	34.0	38.0	23.0	38.0
205-209	33.92336567394918	38.0	34.0	38.0	21.6	38.0
210-214	33.82783215411554	38.0	34.0	38.0	21.0	38.0
215-219	33.66560614035918	38.0	34.0	38.0	19.8	38.0
220-224	33.56582152938349	38.0	34.0	38.0	17.4	38.0
225-229	33.38863992618639	38.0	33.0	38.0	15.0	38.0
230-234	33.301430489860756	38.0	33.0	38.0	15.0	38.0
235-239	33.23391142447935	38.0	33.0	38.0	16.2	38.0
240-244	33.29231871448044	38.0	33.0	38.0	21.0	38.0
245-249	33.721112088508825	38.0	33.0	38.0	22.4	38.0
250-252	35.34194482224657	37.333333333333336	33.666666666666664	38.0	30.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	2.0
18	11.0
19	61.0
20	402.0
21	1725.0
22	4767.0
23	10044.0
24	16750.0
25	24561.0
26	33321.0
27	43328.0
28	53249.0
29	63938.0
30	75311.0
31	88615.0
32	105565.0
33	129558.0
34	166080.0
35	237068.0
36	436086.0
37	917215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.59247122370054	22.561757934010124	12.622241587043161	25.22352925524617
2	18.12674391462575	18.962743047392944	37.33495371850986	25.575559319471452
3	18.68899154281879	26.24774781135859	28.17862836000794	26.884632285814682
4	15.044537056342719	18.2382630755697	37.556330674871596	29.160869193215984
5	15.761582417436362	34.95413385123635	34.86454471523779	14.419739016089494
6	29.266146077995263	38.45540419137428	17.99399333543496	14.2844563951955
7	26.747392384054386	29.693241443244244	24.413257857528826	19.14610831517254
8	25.50685126091361	34.150184889139204	19.791257815164023	20.551706034783166
9	25.15563873455502	15.185165341105197	20.375269089185803	39.283926835153984
10-14	25.411331089660123	21.72943926029392	26.79147669998119	26.067752950064772
15-19	25.3529922634393	25.884604090529432	24.01278586377888	24.749617782252386
20-24	25.116802904879354	24.886644446342103	24.930180754177442	25.066371894601097
25-29	25.369170066067454	24.615649031151047	24.97833762012123	25.03684328266027
30-34	25.134441807119845	24.815426504785478	24.730140333837998	25.319991354256675
35-39	25.19301944336802	24.550760718191405	25.029757120921193	25.226462717519382
40-44	25.168680448518664	24.601714777715614	24.82737796340761	25.402226810358115
45-49	25.16302051243669	24.54315255699581	25.069476697073462	25.224350233494036
50-54	24.87512603693362	24.691119174742802	24.951832251798407	25.481922536525165
55-59	24.664447370720776	24.617928216292803	25.19533891363819	25.522285499348236
60-64	24.785073270966674	24.385997207522543	24.83148441907896	25.997445102431822
65-69	24.5841601726826	24.611499037033983	24.943353474320244	25.860987315963175
70-74	24.66854803823855	24.47045820444908	24.821319131744442	26.039674625567926
75-79	24.460167056958063	24.249644816413223	25.035763441791058	26.25442468483766
80-84	24.31156383356081	24.376592381159664	24.752674701917876	26.559169083361645
85-89	24.081678358484698	24.148499823227155	25.081558073202565	26.68826374508558
90-94	24.049337836917314	24.006256946855824	25.01280962832141	26.93159558790545
95-99	23.81251163007071	23.95942434472859	25.171435549471	27.056628475729706
100-104	23.868396276612756	23.71638568355628	25.402724643253205	27.012493396577756
105-109	23.48234346086028	24.19847432880644	25.221849538830266	27.09733267150301
110-114	23.278293181213787	24.4874717627512	25.311258785418012	26.922976270617006
115-119	23.082905125294417	24.55460534225168	25.27524276938597	27.08724676306793
120-124	23.151073780218244	24.520671224817438	25.16134956274327	27.16690543222105
125-129	24.0289460343833	22.52649751652115	26.82588345171513	26.618672997380422
130-134	22.911608404677505	30.29937693066918	26.267861097339246	20.521153567314066
135-139	25.71239948114789	21.28765889262864	26.108033788840316	26.89190783738315
140-144	24.99689402099875	24.78642855382287	24.226003356122725	25.990674069055657
145-149	25.005229306767124	24.836509221232983	25.08168710093228	25.07657437106761
150-154	25.17084528100465	24.600529687612546	25.099115535768483	25.129509495614315
155-159	25.07397150030966	24.68328472684112	24.76580237020251	25.47694140264671
160-164	25.04304664660877	24.448171804171654	25.173302518074763	25.33547903114482
165-169	25.05658518289297	24.558918707536133	24.839796063503314	25.544700046067582
170-174	25.12796760815885	24.57827460634175	24.847570965823703	25.4461868196757
175-179	25.082453721485138	24.57800450168505	24.759484301564264	25.580057475265548
180-184	24.97886189477029	24.603005816080746	24.945087557637454	25.473044731511507
185-189	24.856603723306808	24.24066591831908	25.053053291452514	25.849677066921593
190-194	24.67163687406898	24.29260068325779	25.26555683910665	25.77020560356658
195-199	24.502596810873236	24.334844902146664	25.472631404759632	25.68992688222047
200-204	24.56748223395496	23.973421838356167	25.502124262151565	25.95697166553731
205-209	24.587475700349486	23.97140263719902	25.423544502077256	26.017577160374234
210-214	24.338791338961872	23.991731061015113	25.423468326236158	26.246009273786854
215-219	24.234015547184594	24.12593324732285	25.29240211658944	26.34764908890312
220-224	24.214588038703024	23.89262631242313	25.781357417685026	26.11142823118882
225-229	24.160169451175594	23.828257028156965	25.81036855580308	26.20120496486436
230-234	24.03377082638631	24.074855235119323	25.712241692234944	26.179132246259424
235-239	23.97302616116089	23.826225055017332	26.23921861344114	25.96153017038063
240-244	23.89870738923978	23.89897736352691	26.071355945857093	26.130959301376215
245-249	23.747097071818153	23.88980355198382	26.152242906105272	26.210856470092757
250-252	23.31661733109172	23.363703430951816	25.90458381871764	27.41509541923882
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.3333333333333333
14	1.6666666666666665
15	1.9999999999999998
16	2.333333333333333
17	3.0
18	1.9999999999999998
19	3.0
20	7.9999999999999964
21	10.333333333333334
22	21.16666666666666
23	44.500000000000036
24	80.83333333333327
25	158.6666666666667
26	249.1666666666692
27	414.9999999999944
28	664.6666666666712
29	1067.0000000000398
30	1665.4999999999045
31	2365.166666666613
32	3177.6666666669794
33	4112.83333333407
34	5235.9999999997535
35	6554.999999998559
36	7972.166666663931
37	9667.999999999605
38	12086.000000003978
39	15888.333333344204
40	21360.8333333275
41	27791.499999970838
42	34723.833333299015
43	41268.00000001312
44	48023.833333395436
45	53694.166666769896
46	57374.00000012995
47	59649.333333479895
48	61238.33333349138
49	61561.83333349359
50	63094.666666837984
51	66878.83333350786
52	69502.83333346999
53	69153.83333347517
54	65341.50000018729
55	60314.833333484115
56	53251.666666766214
57	45703.50000004477
58	38942.833333329225
59	33151.83333328735
60	26913.333333307462
61	21209.83333332817
62	15757.000000010496
63	11507.500000002794
64	8762.666666664509
65	6526.333333331967
66	4526.000000000428
67	2859.666666666812
68	1647.1666666665901
69	951.500000000031
70	722.8333333333409
71	561.666666666658
72	463.83333333332536
73	429.3333333333271
74	380.66666666666305
75	284.1666666666685
76	181.8333333333339
77	113.33333333333292
78	55.16666666666676
79	24.66666666666665
80	13.166666666666671
81	4.0
82	0.6666666666666666
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09893423490027661
2	8.306820730501815E-5
3	8.306820730501815E-5
4	8.306820730501815E-5
5	8.306820730501815E-5
6	4.1534103652509074E-5
7	4.1534103652509074E-5
8	4.1534103652509074E-5
9	4.1534103652509074E-5
10-14	4.1534103652509074E-5
15-19	4.1534103652509074E-5
20-24	5.980910925961307E-4
25-29	0.0012377162888447704
30-34	4.9840924383010895E-5
35-39	4.1534103652509074E-5
40-44	4.1534103652509074E-5
45-49	7.559206864756651E-4
50-54	0.001345704958341294
55-59	0.00206009154116445
60-64	0.002026864258242443
65-69	0.004086955799406893
70-74	0.011106219316680927
75-79	0.012667945810764313
80-84	0.003306145409923819
85-89	6.728620369494296E-4
90-94	0.001304205847876267
95-99	2.5752233113201584E-4
100-104	0.0027746272466900317
105-109	3.904506731203455E-4
110-114	3.904661135229548E-4
115-119	4.403432883042164E-4
120-124	0.0013627732235669359
125-129	0.03321023108113157
130-134	0.026718555116490905
135-139	0.013013743112207939
140-144	0.01069075249908829
145-149	0.009491789601994274
150-154	0.011781475690194648
155-159	0.016885409598989008
160-164	0.020374092314204638
165-169	0.015686499153062267
170-174	0.01316371869308003
175-179	0.018817326061297192
180-184	0.007668584589941798
185-189	0.009042640797497637
190-194	0.0078439248801803
195-199	0.006054125715960741
200-204	0.004581082876370316
205-209	4.999688769374106E-4
210-214	0.0011340892983581305
215-219	9.101408731073245E-4
220-224	0.001280365799672126
225-229	7.557529806267761E-4
230-234	3.8017506977479777E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	19.0
80-84	11.0
85-89	20.0
90-94	42.0
95-99	22.0
100-104	47.0
105-109	73.0
110-114	130.0
115-119	241.0
120-124	809.0
125-129	4170.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	4.0
160-164	2.0
165-169	10.0
170-174	8.0
175-179	27.0
180-184	44.0
185-189	69.0
190-194	148.0
195-199	291.0
200-204	830.0
205-209	1405.0
210-214	2426.0
215-219	4366.0
220-224	6827.0
225-229	11102.0
230-234	20036.0
235-239	33853.0
240-244	74366.0
245-249	297633.0
250-253	1948628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.42394930153735
#Duplication Level	Percentage of total
1	15.675509597129627
2	5.281257540392823
3	3.5287825407205307
4	2.846814991052753
5	2.4043282442925893
6	2.2079508440459974
7	1.9165255233123357
8	1.7866809962003813
9	1.6897892449330425
>10	28.213975697652444
>50	12.40371896759658
>100	19.6769602025474
>500	2.0989091255872063
>1k	0.2687964845363058
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	3.322728292200726E-5	0.0
15-19	0.0	0.0	0.0	4.984092438301089E-5	1.4121595241853085E-4	0.0
20-24	0.0	0.0	0.0	3.405796499505744E-4	1.661364146100363E-4	0.0
25-29	0.0	0.0	0.0	0.007393070450146616	1.8275005607103992E-4	0.0
30-34	0.0	0.0	0.0	0.12084762798734042	2.907387255675635E-4	0.0
35-39	0.0	0.0	0.0	0.4361745429171894	3.1565918775906896E-4	0.0
40-44	0.0	0.0	0.0	1.0909929142819168	3.488864706810762E-4	0.0
45-49	0.0	0.0	0.0	1.856516285522042	4.402614987165962E-4	0.0
50-54	0.0	0.0	0.0	2.7205336301637275	5.980910925961307E-4	0.0
55-59	0.0	0.0	0.0	3.6494023242484404	6.645456584401452E-4	0.0
60-64	0.0	0.0	0.0	4.721837801018416	9.386707425467052E-4	0.0
65-69	0.0	0.0	0.0	5.847428623642873	0.001212795826653265	3.322728292200726E-5
70-74	0.0	0.0	0.0	7.124668765523372	0.0013623185998022976	4.1534103652509074E-5
75-79	0.0	0.0	0.0	8.49313441266624	0.0015533754766038394	4.1534103652509074E-5
80-84	0.0	0.0	0.0	9.979531993720043	0.0017361255326748793	4.1534103652509074E-5
85-89	0.0	0.0	0.0	11.60206175290531	0.0019354892302069228	4.1534103652509074E-5
90-94	0.0	0.0	0.0	13.332821079388287	0.002558500784994559	4.1534103652509074E-5
95-99	0.0	0.0	0.0	15.18787536446176	0.014919050031981263	4.1534103652509074E-5
100-104	0.0	0.0	0.0	17.109193158502446	0.054035868851914304	4.1534103652509074E-5
105-109	0.0	0.0	0.0	19.10457456617629	0.132643313424653	4.1534103652509074E-5
110-114	0.0	3.322728292200726E-5	0.0	21.1605708447206	0.21091848516817158	4.1534103652509074E-5
115-119	0.0	4.1534103652509074E-5	0.0	22.425217846373656	0.26929882126213833	4.1534103652509074E-5
120-124	8.306820730501815E-6	4.1534103652509074E-5	0.0	22.45328659362202	0.27433275462482243	4.984092438301089E-5
125-129	4.1534103652509074E-5	4.1534103652509074E-5	0.0	22.453444423215903	0.27433275462482243	9.968184876602178E-5
130-134	4.1534103652509074E-5	4.1534103652509074E-5	0.0	22.453677014196355	0.27433275462482243	1.661364146100363E-4
135-139	4.1534103652509074E-5	4.1534103652509074E-5	0.0	22.454183730260915	0.27433275462482243	1.661364146100363E-4
140-144	4.1534103652509074E-5	4.1534103652509074E-5	0.0	22.45489811684374	0.27433275462482243	1.661364146100363E-4
145-149	4.1534103652509074E-5	4.1534103652509074E-5	0.0	22.456152446774045	0.2743576750870139	1.9105687680154174E-4
150-154	4.1534103652509074E-5	4.1534103652509074E-5	0.0	22.457739049533572	0.27441582283212745	2.24284159723549E-4
155-159	4.1534103652509074E-5	4.1534103652509074E-5	0.0	22.460314163960028	0.27445735693578	2.5751144264555626E-4
160-164	4.1534103652509074E-5	4.1534103652509074E-5	1.661364146100363E-5	22.463229858036435	0.27452381150162397	3.1565918775906896E-4
165-169	4.1534103652509074E-5	4.1534103652509074E-5	4.1534103652509074E-5	22.466909779620046	0.274590266067468	3.5719329141157804E-4
170-174	4.1534103652509074E-5	4.1534103652509074E-5	4.1534103652509074E-5	22.472467042688752	0.27465672063331203	4.070342157945889E-4
175-179	4.1534103652509074E-5	4.1534103652509074E-5	4.1534103652509074E-5	22.479336783432878	0.274723175199156	4.568751401775998E-4
180-184	4.1534103652509074E-5	4.1534103652509074E-5	4.1534103652509074E-5	22.49011903674107	0.27474809566134756	4.6518196090810163E-4
185-189	4.1534103652509074E-5	4.1534103652509074E-5	4.1534103652509074E-5	22.501158801491904	0.27478962976500004	5.39943347482618E-4
190-194	4.1534103652509074E-5	4.1534103652509074E-5	4.1534103652509074E-5	22.51282988461826	0.274889311613766	5.81477451135127E-4
195-199	4.1534103652509074E-5	4.1534103652509074E-5	4.1534103652509074E-5	22.526477991078472	0.2750554480283761	6.230115547876361E-4
200-204	4.1534103652509074E-5	4.1534103652509074E-5	4.1534103652509074E-5	22.54163793891164	0.27577814143192975	6.230115547876361E-4
205-209	4.1534103652509074E-5	4.1534103652509074E-5	4.1534103652509074E-5	22.55817681898607	0.279142403827783	6.230115547876361E-4
210-214	4.1534103652509074E-5	4.1534103652509074E-5	4.1534103652509074E-5	22.574998130965337	0.2834121096832609	6.479320169791416E-4
215-219	4.1534103652509074E-5	8.306820730501815E-5	4.1534103652509074E-5	22.594303182343022	0.28950100927871875	6.645456584401452E-4
220-224	4.1534103652509074E-5	8.306820730501815E-5	4.1534103652509074E-5	22.608831811800673	0.29908708040171783	6.645456584401452E-4
225-229	4.1534103652509074E-5	8.306820730501815E-5	4.1534103652509074E-5	22.62792088583936	0.3088808220429795	6.645456584401452E-4
230-234	4.1534103652509074E-5	8.306820730501815E-5	4.1534103652509074E-5	22.65055697232998	0.3165064834735802	6.645456584401452E-4
235-239	4.1534103652509074E-5	8.306820730501815E-5	4.1534103652509074E-5	22.6781771512589	0.32083433707417164	6.645456584401452E-4
240-241	4.1534103652509074E-5	8.306820730501815E-5	4.1534103652509074E-5	22.727191546979224	0.3211416894412002	6.645456584401452E-4
>>END_MODULE
