##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665128
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1835938
Total Bases	457.9 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.889198328048113	32.0	25.0	33.0	18.0	33.0
2	28.737337535363395	31.0	27.0	33.0	18.0	33.0
3	30.873699438652068	33.0	30.0	33.0	27.0	33.0
4	31.13457643994514	33.0	31.0	33.0	28.0	33.0
5	32.125120238265126	33.0	33.0	33.0	31.0	34.0
6	35.925086250189274	37.0	36.0	38.0	31.0	38.0
7	36.2296635289427	38.0	37.0	38.0	33.0	38.0
8	36.638785187735095	38.0	37.0	38.0	34.0	38.0
9	36.75787907870527	38.0	38.0	38.0	34.0	38.0
10-14	36.863043305383954	38.0	38.0	38.0	35.0	38.0
15-19	36.83243192308237	38.0	38.0	38.0	35.0	38.0
20-24	36.823746880341275	38.0	38.0	38.0	34.8	38.0
25-29	36.77985563782655	38.0	38.0	38.0	35.0	38.0
30-34	36.7479914899087	38.0	38.0	38.0	34.6	38.0
35-39	36.697457103671255	38.0	38.0	38.0	34.2	38.0
40-44	36.71534093199226	38.0	38.0	38.0	34.0	38.0
45-49	36.67296706097919	38.0	38.0	38.0	34.0	38.0
50-54	36.5727658559276	38.0	38.0	38.0	34.0	38.0
55-59	36.47990378760067	38.0	38.0	38.0	34.0	38.0
60-64	36.47061523864096	38.0	38.0	38.0	34.0	38.0
65-69	36.41817316271029	38.0	38.0	38.0	34.0	38.0
70-74	36.35599208687875	38.0	37.6	38.0	33.6	38.0
75-79	36.300648148972655	38.0	37.0	38.0	33.0	38.0
80-84	36.245293614936955	38.0	37.0	38.0	33.0	38.0
85-89	36.15858746482782	38.0	37.0	38.0	33.0	38.0
90-94	36.08947346006228	38.0	37.0	38.0	33.0	38.0
95-99	36.017517386461826	38.0	37.0	38.0	32.6	38.0
100-104	35.928224788252834	38.0	37.0	38.0	31.6	38.0
105-109	35.800098811256056	38.0	37.0	38.0	31.0	38.0
110-114	35.717624237993434	38.0	36.0	38.0	31.0	38.0
115-119	35.59653993624211	38.0	36.0	38.0	31.0	38.0
120-124	35.65899661352431	38.0	36.0	38.0	31.0	38.0
125-129	33.00858500203513	34.4	33.0	35.4	28.4	35.6
130-134	33.94243706656025	36.0	34.4	36.4	27.6	37.2
135-139	35.20873204726706	38.0	36.0	38.0	28.0	38.0
140-144	35.1053237581775	38.0	36.0	38.0	27.8	38.0
145-149	35.07495597519393	38.0	36.0	38.0	27.0	38.0
150-154	35.041081534980904	38.0	36.0	38.0	27.0	38.0
155-159	35.02759060900319	38.0	36.0	38.0	27.0	38.0
160-164	34.98497335242674	38.0	36.0	38.0	27.0	38.0
165-169	34.95084888243403	38.0	36.0	38.0	27.0	38.0
170-174	34.904795040582485	38.0	36.0	38.0	27.0	38.0
175-179	34.860385082673375	38.0	36.0	38.0	25.4	38.0
180-184	34.76339961047328	38.0	35.0	38.0	25.0	38.0
185-189	34.697081643152735	38.0	35.0	38.0	25.0	38.0
190-194	34.65429164844882	38.0	35.0	38.0	25.0	38.0
195-199	34.597105421228285	38.0	35.0	38.0	25.0	38.0
200-204	34.509816095774205	38.0	35.0	38.0	25.0	38.0
205-209	34.44315441421166	38.0	35.0	38.0	24.6	38.0
210-214	34.37764948036734	38.0	34.6	38.0	23.6	38.0
215-219	34.257243295358975	38.0	34.0	38.0	23.0	38.0
220-224	34.17251660613347	38.0	34.0	38.0	23.0	38.0
225-229	34.033317397993926	38.0	34.0	38.0	23.0	38.0
230-234	33.96699548873899	38.0	34.0	38.0	22.4	38.0
235-239	33.90764983333803	38.0	34.0	38.0	21.6	38.0
240-244	33.949779749631766	38.0	34.0	38.0	23.0	38.0
245-249	34.32252136776834	38.0	34.0	38.0	24.4	38.0
250-252	35.71969270171261	37.666666666666664	34.666666666666664	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	8.0
18	10.0
19	34.0
20	200.0
21	861.0
22	2651.0
23	5567.0
24	9943.0
25	15318.0
26	21053.0
27	28394.0
28	35462.0
29	43387.0
30	51468.0
31	60918.0
32	72599.0
33	89677.0
34	117095.0
35	168025.0
36	313386.0
37	799881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.02432453505472	28.282915301960216	13.540541527878725	22.152218635106347
2	14.554670568043605	22.36399694324357	43.7475659514808	19.333766537232023
3	14.314061056594566	33.753845449950326	32.631093894340545	19.300999599114565
4	10.796890523416938	21.165389207466927	45.63492409321458	22.402796175901557
5	10.876522928903839	39.62365790528646	39.32135978596204	10.178459379847665
6	22.504136849937197	46.452821391572044	20.866881125615354	10.176160632875401
7	20.043051562743404	34.71304586538325	30.842926068309495	14.400976503563845
8	19.443695508070267	40.532382102435975	23.575046420438177	16.44887596905558
9	21.774210240215083	17.606476907172247	26.086992044393657	34.53232080821901
10-14	20.312036680977243	26.241474385300595	32.82168569962602	20.624803234096138
15-19	19.75995921430898	31.723195445597835	29.086929950793543	19.429915389299637
20-24	19.76700408708571	30.26943522658717	30.118372756422197	19.845187929904924
25-29	19.86706370712837	30.12948842458564	30.087220718583684	19.9162271497023
30-34	19.832179463199562	30.19325168712179	30.123085896065955	19.851482953612688
35-39	19.83483318443577	30.136750820207524	30.014840372043107	20.0135756233136
40-44	19.797104171101275	29.747444520433486	30.313683932108354	20.14176737635688
45-49	19.73679322700149	29.707830176542032	30.370863652738834	20.184512943717646
50-54	19.624874109232607	29.754602196068774	30.30176200775961	20.31876168693901
55-59	19.538618727792283	29.414338849633037	30.339081889113412	20.70796053346127
60-64	19.59275221520249	29.298159533797076	30.18583102693147	20.923257224068966
65-69	19.3764942500311	29.384758448143515	30.01687264702941	21.22187465479597
70-74	19.394102814261366	29.010331977489397	29.755419698794555	21.840145509454683
75-79	19.193599206503116	28.913554685610414	29.784056674364273	22.108789433522194
80-84	19.05294725276114	28.848845921971485	29.660645260531176	22.4375615647362
85-89	18.943551675457456	28.501007626456058	29.733123604974697	22.82231709311179
90-94	18.953332055097764	28.292487896706948	29.659299381653543	23.094880666541748
95-99	18.857915528506247	28.020730588413954	29.643384027908056	23.477969855171743
100-104	18.946017619864914	27.74129699689224	29.622774175456517	23.68991120778633
105-109	18.873479117277924	27.827795983823734	29.466514433133455	23.832210465764884
110-114	18.598337906679337	28.26591488455797	29.244664677825877	23.891082530936817
115-119	18.55037303451783	28.11417330684779	29.313944015349154	24.02150964328523
120-124	18.475941129282123	28.449555910758654	29.190284058221195	23.884218901738027
125-129	19.58672071457677	27.67966836344675	31.37298110473922	21.360629817237253
130-134	17.359477490652385	35.64760033786444	31.265174851855775	15.727747319627403
135-139	20.817077975132943	25.60184152085113	31.96319323377079	21.617887270245134
140-144	19.564752529494484	30.427971776218694	29.447619873495988	20.559655820790837
145-149	19.72479952139809	30.199920751266607	30.210048559831097	19.86523116750421
150-154	19.764536620002836	30.026283647695102	30.215201813631914	19.99397791867015
155-159	19.75503778116881	30.043343664098863	30.130328826690167	20.07128972804216
160-164	19.766830686157448	29.86456346783399	30.223325078337776	20.145280767670787
165-169	19.76161873780262	29.891606954735877	30.04838601769026	20.298388289771243
170-174	19.754036720834154	29.757575571767504	30.136206343039916	20.352181364358426
175-179	19.870313803059716	29.588160859327367	30.061313887156345	20.480211450456576
180-184	19.829609133060835	29.457456261942642	30.09200681992669	20.620927785069824
185-189	19.743076637718634	29.14255881855065	30.369067148090018	20.74529739564069
190-194	19.57648071338785	29.156158707772896	30.36848663492745	20.89887394391181
195-199	19.555192008983543	29.000635658991126	30.24497235348772	21.19919997853761
200-204	19.65077731909058	28.732816708748793	30.14486159776118	21.47154437439945
205-209	19.77849632301091	28.410919817650182	30.258109741925328	21.552474117413585
210-214	19.808496566674354	28.27794404233534	30.042709722260145	21.87084966873016
215-219	19.701509317047048	28.351323037588116	29.861167073143154	22.08600057222169
220-224	19.884785124366946	27.937099321954843	30.13744604458994	22.04066950908827
225-229	19.95221267412394	27.98967057552898	29.88303426347293	22.175082486874153
230-234	20.10473187842304	27.778718005544224	29.784151695866935	22.332398420165802
235-239	20.250725996387168	27.611143467287906	29.92302218946253	22.2151083468624
240-244	20.18867245988313	27.365966461146957	29.969808524739143	22.475552554230774
245-249	20.115228214646912	27.713556031054836	29.778048538940087	22.393167215358158
250-252	19.023350712117054	28.022265275773854	30.64573504288635	22.308648969222737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	1.0
12	2.6666666666666665
13	8.666666666666664
14	25.666666666666643
15	52.66666666666675
16	90.99999999999983
17	176.16666666666717
18	285.3333333333349
19	475.8333333333242
20	803.0000000000202
21	1274.8333333333255
22	1838.1666666665321
23	2718.1666666667707
24	4096.333333334056
25	5949.833333332443
26	8516.66666666417
27	11962.33333333706
28	16668.500000011463
29	22781.83333332267
30	29022.999999966647
31	34179.99999996073
32	37731.66666665264
33	41066.166666676574
34	43211.00000002571
35	44222.66666669985
36	44804.00000003707
37	45025.333333371615
38	44240.500000032174
39	42598.16666668733
40	39312.99999999736
41	35715.66666663797
42	31942.33333328984
43	28809.49999996785
44	26505.33333330961
45	24929.99999998215
46	23638.833333320217
47	22585.999999990487
48	21656.66666666031
49	21138.83333332878
50	20708.66666666354
51	19946.333333332917
52	18816.83333333691
53	16695.50000001125
54	14490.833333341528
55	12256.333333337501
56	10144.166666667035
57	8470.99999999735
58	6992.999999998189
59	5870.833333332538
60	5060.166666666598
61	4397.1666666671945
62	3891.6666666672922
63	3286.5000000003583
64	2752.166666666786
65	2289.333333333244
66	1858.8333333331952
67	1443.8333333332878
68	1078.1666666667038
69	804.3333333333535
70	558.8333333333258
71	379.16666666666305
72	255.66666666666944
73	168.33333333333366
74	116.3333333333328
75	66.3333333333335
76	51.66666666666675
77	27.49999999999997
78	12.500000000000004
79	6.833333333333331
80	3.1666666666666665
81	0.6666666666666666
82	0.0
83	0.6666666666666666
84	0.3333333333333333
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10376167386916117
2	2.723403513626277E-4
3	1.0893614054505109E-4
4	1.0893614054505109E-4
5	1.0893614054505109E-4
6	0.0
7	0.0
8	5.4468070272525545E-5
9	0.0
10-14	0.0
15-19	0.0
20-24	4.793190183982248E-4
25-29	0.0011438294757230363
30-34	3.268084216351533E-5
35-39	1.0893614054505108E-5
40-44	4.357445621802043E-5
45-49	6.862976854338218E-4
50-54	0.0011438294757230363
55-59	0.001819233547102353
60-64	0.0018301271611568584
65-69	0.003660254322313717
70-74	0.009858720719327124
75-79	0.011634392484269604
80-84	0.002930401015029907
85-89	5.882643228473039E-4
90-94	0.001078499630532172
95-99	1.960932985768856E-4
100-104	0.002451198543486931
105-109	4.139921502730496E-4
110-114	2.505837238898569E-4
115-119	2.3970732171849665E-4
120-124	0.0010244042517135615
125-129	0.034382365355869506
130-134	0.028267839420717304
135-139	0.012798258823709734
140-144	0.009918924289376406
145-149	0.008605159482836484
150-154	0.010948040054499343
155-159	0.015973197434522756
160-164	0.019618919995971117
165-169	0.01574331607006104
170-174	0.011966278523507935
175-179	0.017451384100584787
180-184	0.0067988802539825175
185-189	0.008353696609286004
190-194	0.006853975075618177
195-199	0.005299614934796669
200-204	0.0038552042245152654
205-209	2.958721236298381E-4
210-214	9.211450490920964E-4
215-219	8.234127648520882E-4
220-224	0.0010119974497664265
225-229	6.730874798556503E-4
230-234	2.9963890183008345E-4
235-239	3.362650037241349E-5
240-244	3.425010200822048E-5
245-249	3.60125554173207E-5
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	7.0
80-84	11.0
85-89	27.0
90-94	21.0
95-99	22.0
100-104	36.0
105-109	66.0
110-114	92.0
115-119	227.0
120-124	989.0
125-129	7629.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	3.0
160-164	0.0
165-169	4.0
170-174	8.0
175-179	14.0
180-184	31.0
185-189	45.0
190-194	89.0
195-199	205.0
200-204	901.0
205-209	1094.0
210-214	1641.0
215-219	2924.0
220-224	4541.0
225-229	7877.0
230-234	14684.0
235-239	24037.0
240-244	51464.0
245-249	203545.0
250-253	1513704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.23108922833694
#Duplication Level	Percentage of total
1	24.342918386449373
2	10.837210345292863
3	6.406569547583117
4	4.7389562982137745
5	3.9508204696171827
6	3.4244759284679356
7	3.0612612997217585
8	2.50605036057347
9	2.3962327215635866
>10	30.463602088279085
>50	5.454870946316698
>100	2.4170316079212086
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.4468070272525545E-5	0.0	0.0
8	0.0	0.0	0.0	5.4468070272525545E-5	0.0	0.0
9	0.0	0.0	0.0	5.4468070272525545E-5	0.0	0.0
10-14	0.0	0.0	0.0	1.307233686540613E-4	0.0	0.0
15-19	0.0	0.0	0.0	3.050211935261431E-4	0.0	5.4468070272525545E-5
20-24	0.0	0.0	0.0	5.119998605617401E-4	5.4468070272525545E-5	5.4468070272525545E-5
25-29	0.0	0.0	0.0	0.003017531093097915	5.4468070272525545E-5	5.4468070272525545E-5
30-34	0.0	0.0	0.0	0.0806236376173923	3.1591480758064817E-4	5.4468070272525545E-5
35-39	0.0	0.0	0.0	0.37121079252131606	3.7038287785317377E-4	5.4468070272525545E-5
40-44	0.0	0.0	0.0	1.0944378295999102	3.921701059621839E-4	5.4468070272525545E-5
45-49	0.0	0.0	1.307233686540613E-4	1.998466179141126	4.5753179028921456E-4	1.0893614054505109E-4
50-54	0.0	0.0	1.6340421081757664E-4	3.1023596657403463	5.773615448887708E-4	1.0893614054505109E-4
55-59	0.0	0.0	1.6340421081757664E-4	4.3085768691535336	6.971912994883269E-4	1.6340421081757664E-4
60-64	0.0	0.0	1.6340421081757664E-4	5.694244576886583	8.932763524694189E-4	2.0697866703559708E-4
65-69	0.0	0.0	1.6340421081757664E-4	7.158847412058577	0.001176510317886552	2.1787228109010218E-4
70-74	0.0	0.0	1.6340421081757664E-4	8.788401351243888	0.0013290209146496232	2.1787228109010218E-4
75-79	0.0	0.0	1.6340421081757664E-4	10.490288887751111	0.001503318739521705	2.1787228109010218E-4
80-84	0.0	0.0	1.6340421081757664E-4	12.30665741435713	0.0018410207752113633	2.1787228109010218E-4
85-89	0.0	0.0	1.6340421081757664E-4	14.18643766837442	0.001928169687647404	2.1787228109010218E-4
90-94	0.0	0.0	1.6340421081757664E-4	16.142800029194884	0.0022440844952280525	2.1787228109010218E-4
95-99	0.0	0.0	1.6340421081757664E-4	18.11722400211772	0.010784677913960056	2.1787228109010218E-4
100-104	0.0	6.536168432703066E-5	1.6340421081757664E-4	20.145113832820062	0.047746710400895884	2.1787228109010218E-4
105-109	0.0	1.0893614054505109E-4	1.6340421081757664E-4	22.208211824146566	0.14428591815192016	2.1787228109010218E-4
110-114	0.0	1.0893614054505109E-4	1.6340421081757664E-4	24.262322583878106	0.25276452690668205	2.1787228109010218E-4
115-119	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.55978469861183	0.3468853523376062	2.1787228109010218E-4
120-124	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.603500771812556	0.357299647373713	2.1787228109010218E-4
125-129	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.603827580234196	0.35731054098776754	2.2876589514460728E-4
130-134	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.60394740998879	0.35734322182993106	2.723403513626277E-4
135-139	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.604372260936913	0.35737590267209457	3.1591480758064817E-4
140-144	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.605407154272093	0.35748483881263965	3.2680842163515327E-4
145-149	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.60644204760727	0.3575502004969667	3.2680842163515327E-4
150-154	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.607694813223542	0.35770271109372975	3.594892637986687E-4
155-159	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.610113195543637	0.35784432807643834	4.684254043437196E-4
160-164	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.613359492531885	0.35785522169049283	4.902126324527299E-4
165-169	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.61858842727805	0.3578987961467107	4.902126324527299E-4
170-174	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.625745531711857	0.35796415783103785	5.119998605617401E-4
175-179	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.634122720919766	0.3580077322872559	5.446807027252555E-4
180-184	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.647717951259796	0.35807309397158293	5.88255158943276E-4
185-189	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.66269667058474	0.35818203011212796	6.427232292158015E-4
190-194	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.678481517349713	0.35829096625267304	6.862976854338218E-4
195-199	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.695366619134198	0.35836722155105455	7.625529838153576E-4
200-204	0.0	1.0893614054505109E-4	1.6340421081757664E-4	25.712578529340316	0.3586722427445807	7.95233825978873E-4
205-209	0.0	1.0893614054505109E-4	2.0697866703559708E-4	25.733755715062273	0.3608400719414272	8.714891243604087E-4
210-214	0.0	1.0893614054505109E-4	2.1787228109010218E-4	25.75414856057231	0.36455479433401344	8.714891243604087E-4
215-219	0.0	1.0893614054505109E-4	2.2876589514460728E-4	25.773757065870416	0.3689884952541971	8.714891243604087E-4
220-224	0.0	1.0893614054505109E-4	2.723403513626277E-4	25.791350252568442	0.3793483222200314	8.714891243604087E-4
225-229	0.0	1.0893614054505109E-4	2.723403513626277E-4	25.81026156656706	0.3921918931902929	8.714891243604087E-4
230-234	0.0	1.0893614054505109E-4	2.723403513626277E-4	25.83469594289132	0.4018545288566389	8.714891243604087E-4
235-239	0.0	1.0893614054505109E-4	2.723403513626277E-4	25.864152275294703	0.40780244213039873	8.714891243604087E-4
240-241	0.0	1.0893614054505109E-4	2.723403513626277E-4	25.91141421987017	0.40823818669257894	8.987231594966714E-4
>>END_MODULE
