##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665133
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	428864
Total Bases	105.3 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.910796895985673	32.0	25.0	33.0	18.0	33.0
2	28.66359498582301	31.0	27.0	33.0	18.0	33.0
3	30.873927398895688	33.0	30.0	33.0	27.0	33.0
4	31.220312266825847	33.0	31.0	33.0	28.0	33.0
5	32.03991241978809	33.0	33.0	33.0	31.0	34.0
6	35.57529659752276	37.0	36.0	38.0	29.0	38.0
7	35.92942284733622	38.0	36.0	38.0	31.0	38.0
8	36.45874449708999	38.0	37.0	38.0	34.0	38.0
9	36.664126622892105	38.0	37.0	38.0	34.0	38.0
10-14	36.803332991344575	38.0	38.0	38.0	34.8	38.0
15-19	36.79463885987166	38.0	38.0	38.0	34.8	38.0
20-24	36.810680775257424	38.0	38.0	38.0	34.8	38.0
25-29	36.786646582599616	38.0	38.0	38.0	35.0	38.0
30-34	36.758828439785106	38.0	38.0	38.0	35.0	38.0
35-39	36.70636052454857	38.0	38.0	38.0	34.4	38.0
40-44	36.74381435606626	38.0	38.0	38.0	35.0	38.0
45-49	36.72228352111625	38.0	38.0	38.0	34.6	38.0
50-54	36.652217952544405	38.0	38.0	38.0	34.0	38.0
55-59	36.57693068198776	38.0	38.0	38.0	34.0	38.0
60-64	36.57184235561857	38.0	38.0	38.0	34.0	38.0
65-69	36.51466945232055	38.0	38.0	38.0	34.0	38.0
70-74	36.43163473735264	38.0	38.0	38.0	34.0	38.0
75-79	36.31982106244517	38.0	37.8	38.0	33.2	38.0
80-84	36.10346663963041	38.0	37.0	38.0	32.6	38.0
85-89	35.94656800456752	38.0	37.0	38.0	31.2	38.0
90-94	35.8254142760984	38.0	37.0	38.0	31.0	38.0
95-99	35.697492213000274	38.0	37.0	38.0	30.2	38.0
100-104	35.564290211437346	38.0	36.4	38.0	29.0	38.0
105-109	35.360252737024965	38.0	36.0	38.0	28.2	38.0
110-114	35.208468016399856	38.0	35.2	38.0	28.0	38.0
115-119	34.99785312045147	38.0	34.6	38.0	27.2	38.0
120-124	35.11102612884043	38.0	34.4	38.0	28.8	38.0
125-129	33.357666015748755	34.4	32.8	35.6	28.8	36.8
130-134	34.456951213420766	36.2	35.4	37.0	28.8	38.0
135-139	35.52099290476496	38.0	37.0	38.0	29.0	38.0
140-144	35.420167783399094	38.0	36.6	38.0	28.2	38.0
145-149	35.39655347114692	38.0	36.4	38.0	28.0	38.0
150-154	35.3689517462175	38.0	36.0	38.0	28.0	38.0
155-159	35.355690340442834	38.0	36.0	38.0	28.0	38.0
160-164	35.319375927632834	38.0	36.0	38.0	28.0	38.0
165-169	35.29883584755013	38.0	36.0	38.0	28.0	38.0
170-174	35.257339971791325	38.0	36.0	38.0	27.4	38.0
175-179	35.197525569160504	38.0	36.0	38.0	27.0	38.0
180-184	35.08428374184543	38.0	36.0	38.0	27.0	38.0
185-189	34.981927584481845	38.0	36.0	38.0	27.0	38.0
190-194	34.90149633220494	38.0	36.0	38.0	26.2	38.0
195-199	34.790181072741575	38.0	35.2	38.0	25.0	38.0
200-204	34.636822319964025	38.0	35.0	38.0	25.0	38.0
205-209	34.488324097251066	38.0	35.0	38.0	24.2	38.0
210-214	34.29038403885465	38.0	34.2	38.0	23.0	38.0
215-219	34.01781406079128	38.0	34.0	38.0	23.0	38.0
220-224	33.825342173365904	38.0	34.0	38.0	21.0	38.0
225-229	33.60414533731185	38.0	34.0	38.0	15.0	38.0
230-234	33.455267902992304	38.0	33.0	38.0	15.0	38.0
235-239	33.287901071916096	38.0	32.8	38.0	15.0	38.0
240-244	33.253956364805354	38.0	32.0	38.0	18.6	38.0
245-249	33.68421442232492	38.0	32.6	38.0	22.4	38.0
250-252	35.362727046256566	37.333333333333336	33.666666666666664	38.0	30.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	2.0
6	1.0
7	0.0
8	1.0
9	0.0
10	0.0
11	0.0
12	1.0
13	1.0
14	0.0
15	0.0
16	2.0
17	3.0
18	14.0
19	72.0
20	171.0
21	327.0
22	645.0
23	1291.0
24	2159.0
25	3471.0
26	4924.0
27	6449.0
28	8124.0
29	9955.0
30	12045.0
31	14225.0
32	16996.0
33	21459.0
34	28426.0
35	41511.0
36	80500.0
37	176088.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.956405031499386	25.523025248528903	13.637053272365268	23.883516447606443
2	15.425943717411785	21.74980645281646	41.94680483914597	20.877444990625786
3	16.572498560079097	30.65527477421807	30.53774924622764	22.234477419475194
4	12.883442735720365	19.747694388415397	41.38534901887628	25.983513856987955
5	13.265755839576906	37.14057191359968	37.001592664880455	12.592079581942958
6	26.12729621459784	42.34152096964496	19.59996082619422	11.931221989562978
7	23.54624184454383	31.969621362981343	27.831149995103228	16.6529867973716
8	21.30105490938599	38.95026722505666	22.324251723205208	17.42442614235214
9	22.906989758800155	17.771233234465647	23.799130710541533	35.52264629619266
10-14	22.25200697298946	24.850415571890533	30.45930697289619	22.43827048222382
15-19	21.979348788708197	29.254981040264777	27.232484921519145	21.533185249507877
20-24	21.99840038428086	28.17846674734699	27.977512107878454	21.845620760493688
25-29	22.045040436031044	27.992198674502195	27.917440635447992	22.04532025401877
30-34	21.823154949282966	28.057082896117524	28.139862422758537	21.97989973184097
35-39	21.710647815648535	27.46822668231159	28.54659806776442	22.27452743427545
40-44	21.15264348582805	26.58148153848091	29.525559036147946	22.74031593954309
45-49	20.217736211236957	26.340354604835454	30.516134809159023	22.92577437476857
50-54	19.159036503063337	26.860911907345802	30.164846977774317	23.815204611816544
55-59	18.67830270424548	26.130717478960086	29.895982666264974	25.294997150529458
60-64	19.15058530872989	25.805163849601705	28.489641847300668	26.554608994367733
65-69	19.369090175612875	26.086451621929807	26.892857725823788	27.651600476633533
70-74	20.25448582334307	26.432543813500192	25.898890344053044	27.414080019103693
75-79	21.237008001820882	26.768320326565338	25.273879064109718	26.720792607504062
80-84	21.62854690968789	26.989907281308884	24.917448650262113	26.46409715874112
85-89	21.81762721445572	26.15076139077824	25.395970312079502	26.63564108268654
90-94	22.28158122686091	25.052884348518468	25.523304330709212	27.142230093911408
95-99	22.052832822334892	23.977427740217475	26.576708548011773	27.39303088943586
100-104	21.92410438272109	24.017451559091494	27.01590005016157	27.04254400802585
105-109	21.277049668592834	26.61322323463478	26.27588724593596	25.833839850836434
110-114	20.552841180295864	29.48500320005909	25.30935852489665	24.652797094748397
115-119	20.173740149449355	30.65104490280165	25.122122208961805	24.053092738787196
120-124	20.206335968414812	31.125434242182937	25.026665542750337	23.641564246651914
125-129	21.193728758852455	27.75313751922383	28.41087736619264	22.642256355731075
130-134	20.09873993566439	32.53112092430097	28.88231151379028	18.48782762624436
135-139	22.51133331557051	24.670943700731957	29.45448784071315	23.363235142984383
140-144	21.498327167260438	27.98093465485217	27.823785435888066	22.69695274199933
145-149	21.634126799858407	27.84742072629972	28.46664243383867	22.051810040003197
150-154	21.511819879931373	27.533140672890543	28.681300486535815	22.27373896064227
155-159	21.191462473461783	27.31211988850856	28.84531752126626	22.6511001167634
160-164	20.978067333011676	27.105643567277337	29.325604035755376	22.590685063955608
165-169	20.674590127663866	27.075816568658283	28.996365307745048	23.2532279959328
170-174	20.63009464646984	27.018826520804907	29.073576068317507	23.27750276440775
175-179	20.647284732099056	26.314119050485374	28.93790811575487	24.1006881016607
180-184	20.69978294443815	25.725318613014007	28.986052681411728	24.58884576113611
185-189	20.044365078345475	24.892263375542967	30.169014580427884	24.894356965683677
190-194	19.7597527003195	25.113280651445834	29.555328830342177	25.57163781789249
195-199	19.66405057471046	24.895631591297548	30.09251436597649	25.3478034680155
200-204	20.62565568317484	23.831985100306728	29.262315652725906	26.28004356379253
205-209	21.243707556842313	23.00318453273262	29.420344497708008	26.332763412717064
210-214	21.50034909831392	23.130270501049203	28.55543022188517	26.81395017875171
215-219	21.07309483053256	23.297236588112185	28.487268191738575	27.14240038961668
220-224	21.434917289067698	23.09410645699751	29.69993007489081	25.77104617904399
225-229	21.071275335450814	24.100619868294462	28.944857153038008	25.883247643216716
230-234	21.552775321956506	24.764656928974986	28.579090638764104	25.103477110304407
235-239	22.226272359944115	24.165519605707043	28.664201475190954	24.944006559157884
240-244	22.082822845506563	24.210534301259795	28.470400556610098	25.236242296623544
245-249	21.58898567599566	25.64768083455215	28.258679862860447	24.504653626591747
250-252	19.67702755572165	27.373535062159494	27.940532428628988	25.008904953489864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.5
9	1.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	0.6666666666666666
22	1.0
23	1.0
24	4.333333333333334
25	11.0
26	21.333333333333332
27	35.66666666666663
28	82.66666666666663
29	211.66666666666808
30	435.4999999999937
31	886.33333333336
32	1599.4999999999304
33	2626.3333333333753
34	4053.333333334014
35	5523.833333332896
36	7479.33333333112
37	9572.166666665918
38	11747.833333336474
39	13490.000000006274
40	14413.166666674568
41	14606.666666674853
42	14105.666666673858
43	13029.83333333844
44	11755.666666669349
45	10362.166666666719
46	9215.999999997835
47	8150.666666664271
48	7104.4999999985375
49	6218.333333332649
50	5512.833333333251
51	4972.1666666670435
52	4507.166666667408
53	4128.500000000601
54	3741.8333333337887
55	3461.8333333336864
56	3160.666666666908
57	2839.000000000119
58	2565.1666666666797
59	2404.499999999948
60	2156.4999999998417
61	1883.1666666665249
62	1745.999999999887
63	1613.999999999916
64	1462.4999999999498
65	1297.3333333333196
66	1162.8333333333499
67	997.3333333333765
68	846.8333333333591
69	648.3333333333364
70	480.66666666665697
71	342.99999999999807
72	218.00000000000173
73	155.33333333333331
74	90.49999999999982
75	67.50000000000013
76	43.50000000000002
77	26.333333333333314
78	18.833333333333343
79	12.666666666666666
80	5.666666666666667
81	1.6666666666666665
82	1.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10329614982838382
2	0.006528876287121325
3	0.004896657215340994
4	0.004430308909118042
5	0.005363005521563945
6	0.0013990449186688553
7	0.002331741531114759
8	0.0027980898373377106
9	0.0018653932248918071
10-14	0.0021452022086255783
15-19	0.001818758394269512
20-24	0.0025649156842262348
25-29	0.003217803312938367
30-34	0.0032644381435606626
35-39	0.003171168482316072
40-44	0.002285106700492464
45-49	0.0034509774660498433
50-54	0.004150499925384271
55-59	0.0028913594985823013
60-64	0.003124533651693777
65-69	0.005363005521563945
70-74	0.010819280704372481
75-79	0.012918034835789391
80-84	0.0024718154738446944
85-89	4.197671132055935E-4
90-94	0.0011194797404486222
95-99	3.732171881443828E-4
100-104	0.0023797014267943183
105-109	3.267844179852807E-4
110-114	7.479994520904014E-4
115-119	0.002579072003001102
120-124	0.005744828829999685
125-129	0.03534985682121751
130-134	0.030207671797213762
135-139	0.016507184430918385
140-144	0.013843200776360951
145-149	0.013462631682852746
150-154	0.015080050330262617
155-159	0.020408017639377486
160-164	0.023547779872499473
165-169	0.01931404687795092
170-174	0.016127075647402185
175-179	0.02078956582633053
180-184	0.009324621472700268
185-189	0.010657141212354481
190-194	0.00965921067594966
195-199	0.006996275316283998
200-204	0.004523800907045891
205-209	5.243178624609383E-4
210-214	0.0011938924283819749
215-219	0.001102495666712684
220-224	0.0012546791085794476
225-229	7.809918303373348E-4
230-234	2.99354292790451E-4
235-239	0.0
240-244	5.418709068426374E-5
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	21.0
80-84	19.0
85-89	36.0
90-94	51.0
95-99	76.0
100-104	117.0
105-109	392.0
110-114	1016.0
115-119	1726.0
120-124	1791.0
125-129	3196.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	3.0
165-169	5.0
170-174	10.0
175-179	5.0
180-184	15.0
185-189	34.0
190-194	78.0
195-199	148.0
200-204	334.0
205-209	579.0
210-214	1195.0
215-219	2237.0
220-224	3692.0
225-229	6729.0
230-234	12085.0
235-239	15584.0
240-244	23865.0
245-249	59954.0
250-253	293871.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.53735602547101
#Duplication Level	Percentage of total
1	53.22750605413794
2	14.422818589111763
3	7.406102932480452
4	5.06152947788559
5	3.5120394865125935
6	2.767171582261736
7	2.0850910833704526
8	1.6970581123150155
9	1.3033475370365164
>10	8.047958661177535
>50	0.35232557647830265
>100	0.11705090723213916
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	2.331741531114759E-4	0.0	0.0
2	0.0	0.0	0.0	4.663483062229518E-4	0.0	0.0
3	0.0	0.0	0.0	4.663483062229518E-4	0.0	0.0
4	0.0	0.0	0.0	4.663483062229518E-4	0.0	0.0
5	0.0	0.0	0.0	4.663483062229518E-4	2.331741531114759E-4	0.0
6	0.0	0.0	0.0	4.663483062229518E-4	2.331741531114759E-4	0.0
7	0.0	0.0	0.0	4.663483062229518E-4	2.331741531114759E-4	0.0
8	0.0	0.0	0.0	4.663483062229518E-4	2.331741531114759E-4	0.0
9	0.0	0.0	0.0	4.663483062229518E-4	2.331741531114759E-4	0.0
10-14	0.0	0.0	0.0	4.663483062229518E-4	2.331741531114759E-4	0.0
15-19	0.0	0.0	0.0	4.663483062229518E-4	7.927921205790181E-4	0.0
20-24	0.0	0.0	0.0	0.001025966273690494	0.0010726011043127892	0.0
25-29	0.0	0.0	0.0	0.03777421280405909	0.0012125055961796746	0.0
30-34	0.0	0.0	0.0	0.664173257722728	0.0017721235636472168	0.0
35-39	0.0	0.0	0.0	2.8281693030890915	0.0018653932248918071	0.0
40-44	0.0	0.0	0.0	7.693068198776302	0.0018653932248918071	0.0
45-49	0.0	0.0	0.0	13.110916281152067	0.0018653932248918071	0.0
50-54	0.0	0.0	0.0	18.72355805103716	0.002285106700492464	0.0
55-59	0.0	0.0	0.0	24.20161169974631	0.002331741531114759	0.0
60-64	0.0	0.0	0.0	29.69967169079242	0.003217803312938367	0.0
65-69	0.0	0.0	0.0	34.896284136696025	0.0038706909416505	0.0
70-74	0.0	0.0	0.0	40.000466348306226	0.004756752723474108	2.331741531114759E-4
75-79	0.0	0.0	0.0	44.8353790479033	0.004896657215340994	4.663483062229518E-4
80-84	0.0	0.0	0.0	49.439682510073126	0.004943292045963289	4.663483062229518E-4
85-89	0.0	0.0	0.0	53.71427771974332	0.005269735860319355	4.663483062229518E-4
90-94	0.0	0.0	0.0	57.71783129383674	0.010166393075660349	4.663483062229518E-4
95-99	0.0	0.0	0.0	61.45649902999552	0.07652775705118639	4.663483062229518E-4
100-104	0.0	0.0	0.0	64.93060737203402	0.32681689300104455	4.663483062229518E-4
105-109	0.0	0.0	0.0	68.14565922996567	0.949018803163707	4.663483062229518E-4
110-114	0.0	0.0	0.0	71.06621213251753	1.5948179376212503	4.663483062229518E-4
115-119	0.0	0.0	0.0	72.73793090583494	2.068208103268169	4.663483062229518E-4
120-124	0.0	0.0	0.0	72.77682435457395	2.1144698552454857	4.663483062229518E-4
125-129	0.0	0.0	0.0	72.7771041635577	2.114656394567975	4.663483062229518E-4
130-134	0.0	0.0	0.0	72.77803686017013	2.114656394567975	4.663483062229518E-4
135-139	0.0	0.0	0.0	72.78046187136248	2.1148895687210865	4.663483062229518E-4
140-144	0.0	0.0	0.0	72.78517198925533	2.1148895687210865	4.663483062229518E-4
145-149	0.0	0.0	0.0	72.79165423071183	2.1148895687210865	4.663483062229518E-4
150-154	0.0	0.0	0.0	72.8018672586181	2.1152626473660647	4.663483062229518E-4
155-159	0.0	0.0	0.0	72.81711684823162	2.1153559170273093	4.663483062229518E-4
160-164	0.0	0.0	0.0	72.83483808386809	2.1153559170273093	4.663483062229518E-4
165-169	0.0	0.0	0.0	72.86155984181465	2.1153559170273093	4.663483062229518E-4
170-174	0.0	0.0	0.0	72.892385464856	2.1153559170273093	4.663483062229518E-4
175-179	0.0	0.0	0.0	72.93417027309357	2.1153559170273093	4.663483062229518E-4
180-184	0.0	0.0	0.0	72.99036524399344	2.1153559170273093	5.12983136845247E-4
185-189	0.0	0.0	0.0	73.04231644530668	2.1153559170273093	6.995224593344276E-4
190-194	0.0	0.0	0.0	73.09296187136249	2.115542456349799	6.995224593344276E-4
195-199	0.0	0.0	0.0	73.14528615132072	2.1161487091478883	6.995224593344276E-4
200-204	0.0	0.0	0.0	73.1954652290703	2.1202059394120285	6.995224593344276E-4
205-209	0.0	0.0	0.0	73.2414938068945	2.1338233099537387	6.995224593344276E-4
210-214	0.0	0.0	0.0	73.28430458140576	2.162783539770184	6.995224593344276E-4
215-219	0.0	0.0	0.0	73.32842113117445	2.1998582301149083	6.995224593344276E-4
220-224	0.0	0.0	0.0	73.3582207879421	2.258431577376511	6.995224593344276E-4
225-229	0.0	0.0	0.0	73.38797380987913	2.3237669750783465	6.995224593344276E-4
230-234	0.0	0.0	0.0	73.42392926428892	2.3752051932547382	6.995224593344276E-4
235-239	0.0	0.0	0.0	73.46193665124608	2.40836255782719	6.995224593344276E-4
240-241	0.0	0.0	0.0	73.51514699298612	2.4103212207133264	6.995224593344276E-4
>>END_MODULE
