##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665135
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2106642
Total Bases	524.4 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.89171677010142	30.0	18.0	33.0	18.0	33.0
2	27.259669179670773	29.0	25.0	31.0	18.0	33.0
3	29.7619951562724	31.0	29.0	33.0	25.0	33.0
4	29.753330656086796	31.0	29.0	33.0	25.0	33.0
5	31.192563330646593	33.0	31.0	33.0	28.0	33.0
6	34.638291650883254	37.0	34.0	38.0	28.0	38.0
7	35.914655646284466	38.0	36.0	38.0	31.0	38.0
8	36.31260176147632	38.0	37.0	38.0	33.0	38.0
9	36.47323797778645	38.0	37.0	38.0	34.0	38.0
10-14	36.65778191073756	38.0	38.0	38.0	34.0	38.0
15-19	36.70007101348971	38.0	38.0	38.0	34.0	38.0
20-24	36.71100310351734	38.0	38.0	38.0	34.0	38.0
25-29	36.648138221871584	38.0	38.0	38.0	34.0	38.0
30-34	36.62018510976236	38.0	38.0	38.0	34.0	38.0
35-39	36.585553691609675	38.0	38.0	38.0	34.0	38.0
40-44	36.55103572415247	38.0	38.0	38.0	34.0	38.0
45-49	36.528463402894275	38.0	38.0	38.0	34.0	38.0
50-54	36.47454356269361	38.0	38.0	38.0	34.0	38.0
55-59	36.38500295731311	38.0	37.6	38.0	33.2	38.0
60-64	36.33434062360857	38.0	37.0	38.0	33.0	38.0
65-69	36.25767539050299	38.0	37.0	38.0	33.0	38.0
70-74	36.1791957057725	38.0	37.0	38.0	33.0	38.0
75-79	36.06748640289249	38.0	37.0	38.0	33.0	38.0
80-84	35.94732360564101	38.0	37.0	38.0	31.6	38.0
85-89	35.80625512455014	38.0	37.0	38.0	31.0	38.0
90-94	35.70325360324581	38.0	36.0	38.0	31.0	38.0
95-99	35.582375123245455	38.0	36.0	38.0	30.0	38.0
100-104	35.457675091145575	38.0	36.0	38.0	29.0	38.0
105-109	35.29534873933091	38.0	35.2	38.0	28.8	38.0
110-114	35.14410324811924	38.0	35.0	38.0	28.0	38.0
115-119	34.98975828867041	38.0	35.0	38.0	28.0	38.0
120-124	35.05330499229247	38.0	34.6	38.0	28.8	38.0
125-129	33.47630324206044	34.2	33.2	35.6	30.8	35.6
130-134	34.94371138780666	36.2	35.4	37.0	32.2	37.2
135-139	36.20356472348041	38.0	37.2	38.0	33.0	38.0
140-144	36.13126247324096	38.0	37.0	38.0	33.0	38.0
145-149	36.107262908254185	38.0	37.0	38.0	33.0	38.0
150-154	36.082587774610396	38.0	37.0	38.0	33.0	38.0
155-159	36.07144664517627	38.0	37.0	38.0	32.6	38.0
160-164	36.05317688569717	38.0	37.0	38.0	32.2	38.0
165-169	35.99384161711774	38.0	37.0	38.0	31.0	38.0
170-174	35.94997972914124	38.0	37.0	38.0	31.0	38.0
175-179	35.8867938241591	38.0	37.0	38.0	31.0	38.0
180-184	35.816842927450736	38.0	37.0	38.0	31.0	38.0
185-189	35.71888954879333	38.0	37.0	38.0	29.4	38.0
190-194	35.61301514723191	38.0	37.0	38.0	29.0	38.0
195-199	35.51264782155331	38.0	36.2	38.0	29.0	38.0
200-204	35.3663123799966	38.0	36.0	38.0	28.6	38.0
205-209	35.243747752770126	38.0	36.0	38.0	28.0	38.0
210-214	35.137181305675746	38.0	35.8	38.0	27.4	38.0
215-219	34.925687866104525	38.0	35.0	38.0	26.2	38.0
220-224	34.77822996597929	38.0	35.0	38.0	25.0	38.0
225-229	34.60353336032159	38.0	35.0	38.0	24.0	38.0
230-234	34.40829987313306	38.0	34.0	38.0	23.0	38.0
235-239	34.23616556906187	38.0	34.0	38.0	23.0	38.0
240-244	34.110039454468655	38.0	33.4	38.0	23.0	38.0
245-249	34.366438248748864	38.0	33.2	38.0	24.6	38.0
250-252	35.62655818902966	37.666666666666664	34.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	11.0
18	23.0
19	38.0
20	176.0
21	635.0
22	1761.0
23	3908.0
24	6799.0
25	11135.0
26	16547.0
27	22940.0
28	30707.0
29	39701.0
30	49906.0
31	63200.0
32	80038.0
33	105161.0
34	144326.0
35	218519.0
36	414480.0
37	896629.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.91982918394162	22.469966394272067	11.878487538053964	24.73171688373235
2	16.828718460897992	22.743328091155973	35.2458973593382	25.18205608860783
3	20.534149927111315	27.329055741449526	28.109446477964646	24.027347853474517
4	15.797838253513907	18.94058282659983	35.48069665769499	29.780882262191273
5	17.454900696800433	35.00117960969872	31.930396856779243	15.613522836721605
6	30.74499960125926	35.91286945228212	18.89717805482875	14.444952891629862
7	25.85498627673805	29.561263850241286	24.832411012407423	19.75133886061324
8	25.23220615187875	33.03889936633722	20.718337865825266	21.01055661595877
9	24.30523283274179	15.82571496519815	19.72101558832283	40.148036613737226
10-14	25.357949258563007	21.883197648713434	27.146202642387202	25.612650450336353
15-19	24.93320649640518	26.355441503587222	24.420238464817466	24.291113535190128
20-24	24.763742307419907	25.34611982187373	25.175022751865956	24.715115118840405
25-29	24.9562895734113	25.27287686381677	24.931158926326095	24.83967463644584
30-34	24.795943826970845	25.20308777728873	25.141300331419203	24.859668064321223
35-39	24.73904642478601	25.015674683572662	25.311423967626034	24.933854924015286
40-44	24.51066051193549	24.78804498919099	25.584953292609004	25.11634120626452
45-49	24.16279167802832	24.767244332401866	25.89810552172589	25.171858467843922
50-54	23.803251507963196	24.864745190439038	25.866702642208615	25.46530065938915
55-59	23.606762691914575	24.715922090927336	25.81807507346769	25.8592401436904
60-64	23.641680332981494	24.708326948757634	25.463349307589205	26.186643410671667
65-69	23.565987956188096	24.803008769406475	25.156291386956113	26.474711887449313
70-74	23.584868670203633	24.76311192703334	24.953349614013447	26.698669788749584
75-79	23.42612568512906	24.825520656311202	25.032697075833294	26.71565658272645
80-84	23.353082947906493	24.785878637898467	25.107553195074956	26.753485219120083
85-89	23.244188008309838	24.465826359493978	25.352361535073513	26.93762409712267
90-94	23.212607665871786	24.13769268054848	25.527105237796604	27.12259441578313
95-99	23.015663525504788	23.991927826703876	25.815420912147225	27.176987735644108
100-104	22.876427538829294	24.18280093147576	25.877365186449445	27.063406343245493
105-109	22.621993191431013	24.91432789666381	25.67247936382202	26.791199548083156
110-114	22.355187576588424	25.63253586408456	25.45816306297477	26.554113496352244
115-119	22.254100610587173	25.80247961875866	25.424526482933935	26.51889328772023
120-124	22.31233520446	25.924902350596934	25.29877192705779	26.46399051788527
125-129	24.34402944865707	23.69845139204168	26.619030627727643	25.338488531573606
130-134	23.392370038016153	29.322227949433525	25.916448414123742	21.36895359842658
135-139	25.543442278415462	21.58937249777288	26.33888886662885	26.5282963571828
140-144	24.639341792869175	25.226474779897874	24.731220544970455	25.402962882262496
145-149	24.589582467584258	25.208934110723536	25.420672962260397	24.78081045943181
150-154	24.700228071937822	25.063777049496867	25.294005019357062	24.941989859208245
155-159	24.54151331943422	24.953134847048357	25.419019782293834	25.086332051223586
160-164	24.459762961023866	24.903879525570975	25.51695938946923	25.11939812393593
165-169	24.388570203082185	24.844976360200725	25.462809924451285	25.3036435122658
170-174	24.29067778750562	24.898952417505036	25.523903345019143	25.286466449970202
175-179	24.301467820814675	24.63527725244566	25.514950825383863	25.548304101355797
180-184	24.27407321187327	24.51546364212278	25.613870719066988	25.596592426936965
185-189	24.003219580361133	24.27868338016628	26.090584664305982	25.6275123751666
190-194	23.88500488888766	24.320438890144732	25.999826906563612	25.794729314403998
195-199	23.69533594613852	24.287149109889565	26.30036939769261	25.717145546279312
200-204	23.867409697333073	24.027631651807962	26.0814074938277	26.023551157031267
205-209	23.923893079671625	23.829494173781697	26.27418697270257	25.972425773844108
210-214	23.883572541079243	24.04399262796652	25.98390173377499	26.08853309717925
215-219	23.59918979951605	24.124548180665194	26.01456833783626	26.261693681982496
220-224	23.67607745911011	23.89124914248577	26.552665214408645	25.88000818399547
225-229	23.402094941337616	24.19009959900091	26.423194264535443	25.984611195126035
230-234	23.48397339814646	24.350015908000717	26.33767513222609	25.828335561626737
235-239	23.622636162038216	24.1428896490425	26.55070062595742	25.683773562961864
240-244	23.522063293948612	23.86755782504774	26.639520981904692	25.970857899098952
245-249	23.330472237446713	24.473247787960933	26.456329453206706	25.739950521385644
250-252	22.52863902261479	24.452465913965987	26.659512383039345	26.359382680379877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.3333333333333333
16	3.0
17	3.3333333333333335
18	3.6666666666666665
19	8.666666666666664
20	17.33333333333334
21	36.49999999999998
22	58.333333333333464
23	99.16666666666637
24	175.5000000000005
25	266.66666666666913
26	407.4999999999946
27	685.5000000000068
28	1014.833333333377
29	1474.3333333332819
30	2159.666666666518
31	3150.1666666669653
32	4332.333333333907
33	6103.833333332319
34	8259.333333330374
35	10922.666666668445
36	14156.000000007596
37	18116.333333339593
38	22391.666666657464
39	27195.999999973392
40	32010.83333328933
41	36784.49999997945
42	41065.500000010325
43	45038.16666670565
44	47765.50000005876
45	49332.33333340326
46	49773.333333406306
47	48901.3333334001
48	46990.33333338603
49	44599.666666701894
50	42125.66666668407
51	39518.833333332055
52	37107.8333333148
53	34881.49999996546
54	33191.66666661988
55	31796.499999957105
56	30499.999999961827
57	29769.166666631052
58	28842.499999967706
59	27736.66666663833
60	26576.666666642475
61	24671.49999998275
62	22463.166666657362
63	19860.000000000065
64	16852.16666667761
65	13643.000000006612
66	10706.333333334656
67	8048.166666663928
68	5873.66666666589
69	4126.833333334045
70	2730.8333333334226
71	1760.6666666665585
72	1112.0000000000373
73	680.8333333333371
74	400.8333333333292
75	249.66666666666896
76	149.16666666666623
77	80.16666666666671
78	47.83333333333337
79	20.333333333333336
80	9.499999999999996
81	6.166666666666666
82	4.166666666666666
83	1.5
84	0.6666666666666666
85	0.3333333333333333
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035649151588167324
2	5.221580126096413E-4
3	2.373445511862006E-4
4	5.696269228468814E-4
5	6.170958330841216E-4
6	4.746891023724012E-4
7	0.0
8	4.746891023724012E-5
9	4.746891023724012E-5
10-14	7.879839099381859E-4
15-19	0.0
20-24	7.595025637958419E-5
25-29	0.002411420640051798
30-34	0.0030949729474680555
35-39	0.0028671221783293033
40-44	0.011943177815689614
45-49	0.006702610125498306
50-54	0.001215204102073347
55-59	7.595025637958419E-5
60-64	1.1392538456937628E-4
65-69	0.0
70-74	6.740585253688097E-4
75-79	7.405206239740704E-4
80-84	0.00244945553255578
85-89	0.0017469303632732195
90-94	0.0046902449266788444
95-99	0.0023356876938691994
100-104	0.0015666778074866312
105-109	0.0011679509696082065
110-114	5.033063906141904E-4
115-119	0.0012917086551128393
120-124	9.690268614245986E-4
125-129	0.0014577387252008155
130-134	0.0030241050747726672
135-139	0.0026520542927028438
140-144	0.00140234525549395
145-149	0.0017934755648494052
150-154	0.0020605899297252974
155-159	0.001984276516564416
160-164	0.0022132357220061454
165-169	0.0021750870678767403
170-174	0.002871522548034562
175-179	0.003711082054218432
180-184	0.0044076306581050505
185-189	0.003472873849860985
190-194	0.002662171064433318
195-199	0.0015842258441824174
200-204	0.0015752714837195694
205-209	0.00213061816780978
210-214	0.005080141868941141
215-219	0.013657509932865283
220-224	0.014642393473940103
225-229	0.016731319791292276
230-234	0.030936230020107085
235-239	0.07950247198350933
240-244	0.03329521258059912
245-249	3.5401511065225033E-4
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	36.0
80-84	38.0
85-89	44.0
90-94	55.0
95-99	64.0
100-104	96.0
105-109	146.0
110-114	258.0
115-119	422.0
120-124	1147.0
125-129	7848.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	4.0
155-159	4.0
160-164	7.0
165-169	15.0
170-174	25.0
175-179	35.0
180-184	89.0
185-189	167.0
190-194	287.0
195-199	530.0
200-204	1292.0
205-209	2050.0
210-214	4219.0
215-219	6550.0
220-224	10005.0
225-229	15561.0
230-234	25543.0
235-239	35820.0
240-244	67304.0
245-249	245284.0
250-253	1681697.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.26518459823218
#Duplication Level	Percentage of total
1	89.35435986423008
2	8.719214572127596
3	1.2850065224385556
4	0.27408929389902564
5	0.13701707776678312
6	0.06619776032397465
7	0.03444714742333741
8	0.03054642160350931
9	0.0180280041740674
>10	0.0746928655336563
>50	0.006400470479445903
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.746891023724012E-5	0.0
3	0.0	0.0	0.0	4.746891023724012E-5	4.746891023724012E-5	0.0
4	0.0	0.0	0.0	4.746891023724012E-5	4.746891023724012E-5	0.0
5	0.0	0.0	0.0	4.746891023724012E-5	4.746891023724012E-5	0.0
6	4.746891023724012E-5	0.0	0.0	4.746891023724012E-5	4.746891023724012E-5	0.0
7	4.746891023724012E-5	4.746891023724012E-5	0.0	4.746891023724012E-5	4.746891023724012E-5	0.0
8	4.746891023724012E-5	4.746891023724012E-5	0.0	4.746891023724012E-5	4.746891023724012E-5	0.0
9	4.746891023724012E-5	4.746891023724012E-5	0.0	1.8987564094896049E-4	4.746891023724012E-5	0.0
10-14	4.746891023724012E-5	4.746891023724012E-5	3.7975128189792096E-5	3.132948075657848E-4	4.746891023724012E-5	3.7975128189792096E-5
15-19	4.746891023724012E-5	5.696269228468814E-5	1.234191666168243E-4	7.595025637958418E-4	9.493782047448024E-5	4.746891023724012E-5
20-24	5.696269228468814E-5	1.1392538456937628E-4	1.4240673071172036E-4	0.003769031472836865	9.493782047448024E-5	4.746891023724012E-5
25-29	9.493782047448024E-5	1.4240673071172036E-4	1.4240673071172036E-4	0.04808600607032424	9.493782047448024E-5	4.746891023724012E-5
30-34	9.493782047448024E-5	1.4240673071172036E-4	2.1835698709130454E-4	0.401662930863431	4.272201921351611E-4	9.493782047448024E-5
35-39	1.4240673071172036E-4	1.7088807685406443E-4	2.5633211528109664E-4	1.2167231072009388	4.746891023724012E-4	1.1392538456937628E-4
40-44	1.8987564094896049E-4	1.8987564094896049E-4	2.9430724347088874E-4	2.72755408844977	5.411455767045374E-4	1.4240673071172036E-4
45-49	2.2785076913875256E-4	1.9936942299640848E-4	3.3228237166068084E-4	4.4331310208379024	7.974776919856339E-4	1.4240673071172036E-4
50-54	3.132948075657848E-4	2.373445511862006E-4	3.4177615370812887E-4	6.236342007801991	0.001500017563496788	1.4240673071172036E-4
55-59	3.7975128189792097E-4	3.0380102551833677E-4	3.7975128189792097E-4	8.070521711804854	0.0022974952554824218	1.613942948066164E-4
60-64	4.0823262804026505E-4	3.512699357555769E-4	3.9873884599281697E-4	9.959186230978021	0.0033797864088914963	2.753196793759927E-4
65-69	4.557015382775051E-4	3.89245063945369E-4	4.272201921351611E-4	11.864303474439415	0.004139288972687338	2.848134614234407E-4
70-74	4.746891023724012E-4	4.272201921351611E-4	4.651953203249532E-4	13.833589190759515	0.0048513226262459404	3.227885896132328E-4
75-79	4.746891023724012E-4	4.3671397418260907E-4	5.411455767045375E-4	15.816868741817547	0.0054019619849979255	3.3228237166068084E-4
80-84	4.746891023724012E-4	4.746891023724012E-4	6.455771792264656E-4	17.848414680804808	0.005791207048943294	3.3228237166068084E-4
85-89	5.031704485147453E-4	4.746891023724012E-4	7.120336535586018E-4	19.892084179466657	0.006484253138407001	3.3228237166068084E-4
90-94	5.221580126096413E-4	4.746891023724012E-4	7.120336535586018E-4	21.964272999399043	0.010120371662579595	3.607637178030249E-4
95-99	5.506393587519854E-4	4.841828844198492E-4	7.595025637958419E-4	24.065389373230005	0.043177720751793616	3.9873884599281697E-4
100-104	5.696269228468814E-4	5.221580126096413E-4	7.595025637958419E-4	26.176009022890458	0.11777036629859272	4.272201921351611E-4
105-109	5.696269228468814E-4	5.221580126096413E-4	7.595025637958419E-4	28.29035023511351	0.24100915105651555	4.272201921351611E-4
110-114	6.550709612739138E-4	5.221580126096413E-4	8.069714740330822E-4	30.384745011254875	0.3493332042178975	4.272201921351611E-4
115-119	7.025398715111537E-4	5.221580126096413E-4	8.069714740330822E-4	31.615870185821798	0.42089733329156076	4.272201921351611E-4
120-124	7.50008781748394E-4	5.221580126096413E-4	8.544403842703222E-4	31.642633157413552	0.4310082111720928	4.557015382775051E-4
125-129	7.974776919856339E-4	5.791207048943295E-4	8.544403842703222E-4	31.642917970874976	0.4310936552105199	5.031704485147453E-4
130-134	8.259590381279781E-4	6.645647433213617E-4	8.544403842703222E-4	31.6435825356183	0.43111264277461475	5.981082689892255E-4
135-139	8.734279483652181E-4	6.740585253688097E-4	8.544403842703222E-4	31.644588876515332	0.4312075805950893	6.360833971790177E-4
140-144	9.683657688396984E-4	7.595025637958419E-4	8.544403842703222E-4	31.646383201322294	0.4313689748898959	6.645647433213617E-4
145-149	0.0010538098072667306	8.069714740330822E-4	8.544403842703222E-4	31.648747153052106	0.43140695001808566	6.645647433213617E-4
150-154	0.001158241409788659	8.35452820175426E-4	8.829217304126662E-4	31.65281998555046	0.4315493567487974	6.645647433213617E-4
155-159	0.001186722755931003	9.493782047448023E-4	9.683657688396984E-4	31.658896006060832	0.4316822696974616	6.645647433213617E-4
160-164	0.001205710320025899	9.493782047448023E-4	9.968471149820425E-4	31.665418234327426	0.43177720751793613	7.120336535586018E-4
165-169	0.0012341916661682431	9.493782047448023E-4	9.968471149820425E-4	31.673079716439712	0.43178670129998353	7.595025637958419E-4
170-174	0.0012816605764054832	9.588719867922504E-4	9.968471149820425E-4	31.68123487521848	0.43186265155636316	8.069714740330822E-4
175-179	0.0014050797430223074	0.0010063408970294905	9.968471149820425E-4	31.690671694573645	0.4319480955947902	8.069714740330822E-4
180-184	0.0014240673071172036	0.0010443160252192826	9.968471149820425E-4	31.705605413734276	0.4320240458511698	8.449466022228743E-4
185-189	0.0014715362173544438	0.0010917849354565227	0.0010348222431718344	31.719950518407966	0.43215695879983407	0.0010253284611243863
190-194	0.0014715362173544438	0.0011297600636463148	0.0010917849354565227	31.735510827183738	0.4326506354663014	0.0011012787175039708
195-199	0.0014715362173544438	0.001158241409788659	0.0010917849354565227	31.752001526600157	0.43367596392742574	0.001196216537978451
200-204	0.0014715362173544438	0.001186722755931003	0.0010917849354565227	31.769707430118643	0.43766335238735393	0.0012626730123105872
205-209	0.0014715362173544438	0.001186722755931003	0.0010917849354565227	31.786160154406872	0.44957804885690117	0.0012911543584529313
210-214	0.001500017563496788	0.001186722755931003	0.0010917849354565227	31.80285022324629	0.4634389706461753	0.0013291294866427234
215-219	0.0015190051275916839	0.0012152041020733468	0.0011107724995514188	31.824334652019655	0.4794929560884099	0.0013291294866427234
220-224	0.00153799269168658	0.0012531792302631392	0.0011392538456937629	31.851895101303406	0.5016229620410112	0.0013291294866427234
225-229	0.001566474037828924	0.0012816605764054832	0.0011392538456937629	31.87808844597231	0.5256802057492445	0.0013291294866427234
230-234	0.001566474037828924	0.0012816605764054832	0.001177228973883555	31.901908345129357	0.5446392884979983	0.0013291294866427234
235-239	0.001566474037828924	0.0012816605764054832	0.001186722755931003	31.93293402486042	0.5569337362494435	0.0013291294866427234
240-241	0.001566474037828924	0.0013053950315241032	0.001186722755931003	31.997415792526684	0.557593554101741	0.0013291294866427234
>>END_MODULE
