FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR5665145

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5665145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15415
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length77-252
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTAAAGAACATTGTGTTGTTATGTGTATTTATCATGCTGTTCCTTTG10126.565034057735971No Hit
GTGTTGGTGCCGGTAACGGTTCTTTCACTTACACCACTAAAGAACATTGT9075.883879338306844No Hit
CCACTAAAGAACATTGTGTTGTTATGTGTGTTTATCATGCTGTTCCTTTG7374.781057411612066No Hit
CATTGTGTTGTTATGTGTGTTTATCATGCTGTTCCTTTGCTTGACTACAC5983.8793383068439833No Hit
GTGTTGTTATGTGTATTTATCATGCTGTTCCTTTGCTTGACTACACAATT4813.1203373337658125No Hit
ACATTGTGTTGTTATGTGTGTTTATCATGCTGTTCCTTTGCTTGACTACA3472.251054168018164No Hit
GTGTTGTTATGTGTGTTTATCATGCTGTTCCTTTGCTTGACTACACAATT3362.179695102173208No Hit
TGACTACACAATTACCGGTCAGGATGGACAGCTGCTTGTTACTGATGCCG1060.6876419072332144No Hit
CATTGTGTTGTTATGTGTATTTATCATGCTGTTCCTTTGCTTGACTACAC1050.6811547194291274No Hit
GAGTTGGTGCCGGTAACGGTTCTTTCACTTACACCACTAAAGAACATTGT990.6422315926046059No Hit
GGTGATACTGCTGTTATTGCTGGTAAAGGTGTTGGTGCCGGTAACGGTTC880.5708725267596496No Hit
ACATTGTGTTGTTATGTGTATTTATCATGCTGTTCCTTTGCTTGACTACA780.5060006487187804No Hit
CAATTACCGGTCAGGATGGACAGCTGCTTGTTACTGATGCCGAGCCCACG780.5060006487187804No Hit
GGTGGTATTTCTCGTAACCTTGATATCAGTGAGGTTGTTAATAACAATCT760.4930262731106066No Hit
ATGTGTACTTATATTGGTGGTATTTCTCGTAACCTTGATATCAGTGAGGT500.3243593902043464No Hit
GAGTTGTTATGTGTATTTATCATGCTGTTCCTTTGCTTGACTACACAATT500.3243593902043464No Hit
GTGTCCCAGTATCCAAGTACAAACAATCCTTGCGTTACTCTCTGCGCCAT480.3113850145961725No Hit
GAACATTGTGTTGTTATGTGTGTTTATCATGCTGTTCCTTTGCTTGACTA420.272461887771651No Hit
CAATCCTTGCGTTACTCTCTGCGCCATTGTAGCTGGTATTGCCGGCGTTG380.2465131365553033No Hit
GCTGCTTGTTACTGATGCCGAGCCCACGAGACGTAGAGGAATCTCGTATG360.23353876094712941No Hit
GATACGTAAACATTTTGGTGTTAATCTGCCTCAAGCTCTTTCCAATATGT360.23353876094712941No Hit
ACTAAAGAACATTGTGTTGTTATGTGTGTTTATCATGCTGTTCCTTTGCT350.22705157314304247No Hit
GGTAAAGGTGTTGGTGCCGGTAACGGTTCTTTCACTTACACCACTAAAGA350.22705157314304247No Hit
GTTATGTGTATTTATCATGCTGTTCCTTTGCTTGACTACACAATTACCGG300.19461563412260785No Hit
GTTATGTGTGTTTATCATGCTGTTCCTTTGCTTGACTACACAATTACCGG300.19461563412260785No Hit
GAGTTGTTATGTGTGTTTATCATGCTGTTCCTTTGCTTGACTACACAATT290.1881284463185209No Hit
GGTAACGGTTCTTTCACTTACACCACTAAAGAACATTGTGTTGTTATGTG270.17515407071034705No Hit
GTGTTTATCATGCTGTTCCTTTGCTTGACTACACAATTACCGGTCAGGAT240.15569250729808626No Hit
CACTAAAGAACATTGTGTTGTTATGTGTGTTTATCATGCTGTTCCTTTGC240.15569250729808626No Hit
GTACCTGTAGGCTCTACTCTTCGTGCTGATTTGTCTACTTTGCAGTCTCA240.15569250729808626No Hit
GGTCAGGATGGACAGCTGCTTGTTACTGATGCCGAGCCCACGAGACGTAG240.15569250729808626No Hit
ATTACCGGTCAGGATGGACAGCTGCTTGTTACTGATGCCGAGCCCACGAG230.14920531949399934No Hit
CTCTCTGCGCCATTGTAGCTGGTATTGCCGGCGTTGCTGATTGTCCGAGC230.14920531949399934No Hit
GGTGTTGGTGCCGGTAACGGTTCTTTCACTTACACCACTAAAGAACATTG230.14920531949399934No Hit
GAGATTAGTCAGTCTGGAGATTCTGATTATCGTGAACAGATACGTAAACA210.1362309438858255No Hit
GTTAATAACAATCTTGCTGCCGAAGGTGATACTGCTGTTATTGCTGGTAA210.1362309438858255No Hit
GCCTCAAGCTCTTTCCAATATGTGTACTTATATTGGTGGTATTTCTCGTA200.12974375608173855No Hit
GTGAGGTTGTTAATAACAATCTTGCTGCCGAAGGTGATACTGCTGTTATT200.12974375608173855No Hit
GAACATTGTGTTGTTATGTGTATTTATCATGCTGTTCCTTTGCTTGACTA180.11676938047356471No Hit
GAGTATGACAGCTGGATTCCTTTTTCGAGTGTCTCCATTGCTTCTCTTGC170.1102821926694778No Hit
GTTATTGCTGGTAAAGGTGTTGGTGCCGGTAACGGTTCTTTCACTTACAC170.1102821926694778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)