FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR5665149

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5665149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486104
Total Bases615.8 Mbp
Sequences flagged as poor quality0
Sequence length75-252
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCGCGCTTATAATCATGCTTTGAGTGTTTTTCCTATTCTTGCTTATA322801.2984171217294207No Hit
GTTCGTCTGCTGCTCCTGCCGCTTCTGCCTCTTCTCCGGCTGTTATGCAG245390.9870463987025482No Hit
TGCATTATTTAACCGATTTTGATAAAATGTTGATTGGTGATGTTAGGCAA219400.8825053175571095No Hit
GGTACAGGTATTAATTATAATTTCTTTAAAGTTAATCCTTCGATATTAGA216670.8715242805610707No Hit
GCATCACACAGGCGTTTTGCCTAACATCACCAATCAACATTTTATCAAAA165950.6675102891914417No Hit
GCATCACACAGGCGTTTTGAATAACTTCACCAAGCGTTATTTTATTAAAA154480.6213738443765828No Hit
GCAATGGACTAATCAGGTTTGGAGTAATAACCTTCTTCGTGCGAATGTTG119830.4819991440422444No Hit
AGGTAATGAGAATTTTTATGCAAAAGATTGGGGTGTTTTGATGTGTATTT112210.45134877704231197No Hit
GCATCACACAGGCGTTTTGTGTATGGCTAGCGTATCATGGCTTATCAAAC92780.3731943635503583No Hit
GGTAATGAGAATTTTTATGCAAAAGATTGGGGTGTTTTGATGTGTATTTA84860.3413372891882238No Hit
GGTTATAATCCTTATATGAATCCCGGTGATGCTGGTAATGCTACCGGTTC82250.33083893513706586No Hit
GGTCAAGGTAATGAGAATTTTTATGCAAAAGATTGGGGTGTTTTGATGTG80870.3252880812709364No Hit
ATCCTTCGATATTAGACCCTATTTTTGCGGTGAATGCTGATTCATATTGG73710.2964879988930471No Hit
GTATCTTCGTTATGGTAATGTCCGTACCGGTGTTGGTACTGGTAGTAGCC70870.28506450253086757No Hit
ATCCTTCGATATTAGACCAAAGTTCCGATGTAATAGGAGCAACCCACTCT64950.26125214391674684No Hit
TCCTTCGATATTAGACCCTATTTTTGCGGTGAATGCTGATTCATATTGGG63470.2552990542632167No Hit
GGTTTTAATCGTGCTGATTTGGCTTATAAGCTTATTCAGTATCTTCGTTA62510.25143759070417004No Hit
CCGTCGTCAAAAGGTTAAGCAAGATGCTGCCTCTGATTTTATGGGTAATC61280.2464900905191416No Hit
CGTTCGCAAATTAAGGCTCATTTTGGTGTTGACGTTGGTTCTGAGTTGTC59890.24089901307427203No Hit
CCTTCGTATATATTTACTAACTATATAAATTTTAACATTATGTATTTATT59740.24029565939317102No Hit
GGTAGTAGCCGTAATTATGGTACATCTGTAGATGTTAAAGACCCTTCTTA57770.23237161438137746No Hit
CCACAGTTGGTGGACACTAATAAAGTGTGTGTGAGTGACCTTGTAGAACG55360.22267773190502085No Hit
GCTTGTTGAATTGTCGTCTTTTACTGATGATTCCGGTGTATTTCATATGT52100.20956484523575844No Hit
ACATGCTTCTTCTTTTGATGGTAGTATTTTACTTGGTTCTAACATGCCTT50250.2021234831688457No Hit
GAATTACAGGATACAGGTGTTGAAGCTTCTGCCGGTGCTGCTTCTGGCGC50210.2019625888538854No Hit
TCCTTCGATATTAGACCAAAGTTCCGATGTAATAGGAGCAACCCACTCTT46320.18631561672399868No Hit
GCAACGCCTTGACAATCGCATTGCTCCTACTGAACTTCGTGAAATGTTTA44220.1778686651885842No Hit
GGTTTGGAGTAATAACCTTCTTCGTGCGAATGTTGCTAACTCTCTTTTGG43760.17601838056654107No Hit
GTTGTGAAACAGGGCTATTTTTTATGTCTATGCGAAGCCTTTACTTTTGT39990.1608540913815351No Hit
GGTTCGTCTGCTGCTCCTGCCGCTTCTGCCTCTTCTCCGGCTGTTATGCA39200.15767642866106968No Hit
GGTACTGGTAGTAGCCGTAATTATGGTACATCTGTAGATGTTAAAGACCC38480.15478033099178473No Hit
CTTAAGAATCAGCTCCACATGCAGGAACTTGTATTTTCCGAATTGGGTTA36920.148505452708334No Hit
CCCTATTTTTGCGGTGAATGCTGATTCATATTGGGACACTGATACCTTCT33990.13671994413749386No Hit
GCTCTTCAACGTTTCCGCGAGATTTCGCTTTGTACCTGCTTCAATCAATT33620.1352316717241113No Hit
CTTTGTTGTTTAATCAGCAACGCCTTGACAATCGCATTGCTCCTACTGAA33620.1352316717241113No Hit
ATATTACGACTCTTTGCCCGAAACGAGCCTTTTAAGGAAGCAACTCCGAG32210.12956014712176162No Hit
ACTTCGTTCCCTGTTCCGGAGCTTGATGCCATTGGGTTAGAGCCTATTAC32070.12899701701940064No Hit
GCATCACACAGGCGTTTTGAATAACTTCACCAATCAACATTTTATCAAAA31750.12770986249971844No Hit
CATTAAAAATTTAGGTTATGGATACAGATTTTACTGTTTGTTTAGAGCTT31570.1269858380823972No Hit
GATGTGTATTTATCATTCTGTACCTCTTCTTGATTATGTTATTTCCGCTC31150.12529644777531432No Hit
GCTCTTCAACGTTTCCGCGAGATTTCGCTTTGTACTCCTCTGAATTATCG30900.1242908583068126No Hit
GGTTTGGAGTAATAACCTTCTTCGTGCGAATGTTGCTAACTCTCTTTTCG30690.12344616315327114No Hit
GCATCACACAGGCGTTTTGAATAACTTCACCAAGCGTTATTTTATCAAAA29290.1178148621296615No Hit
GCATCACACAGGCGTTTTGAATAACTTCACCAAGCGTTATTTTATTAAAC28520.11471764656667621No Hit
AATCTTGCGCGTGCGTTTCGCGCGTGCATTATTTAACCGATTTTGATAAA28250.11363160994069435No Hit
CACAAAACTGTTTCCTTGTTGTATTCGTGATTTAGGTTTTAAGAGATTAT27430.11033327648400872No Hit
GCTTATAAATAGAGGGCAACTTCATGTTCGTGAAGCTAGAGAGAGTTTGT27430.11033327648400872No Hit
GTGCACAGCAATGCAAGAAAATAATTTTTTCTTTTTTCTTTCCGACATTG27300.10981036996038782No Hit
CCCGTACCCAACCTGTTAATACTTCTGCCTATACTCGTGTGCGAGAGTAT27010.10864388617692583No Hit
GCATCACACAGGCGTTTTGAATAACTTCTGAAAAATAACTACGCAATCCT26940.10836232112574536No Hit
GACAAAAGGTCTGCTTTATTTGATGGACTGCCTTATATTTCGAATCAAAA26300.10578801208638094No Hit
GGTGGCTCTTCTGATTGATAGTTTGGATTCTTGTTCGTGCCAATTGGCTA26290.10574778850764087No Hit
TGCATTATTTAACCGATTTTAATAAAATAACGCTTGGTGAAGTTATTCAA25560.10281146725961586No Hit
TGCATTATTTAACCGATTTTGATAAAATGTTGATTGGTGAAGTTATTCAA25520.10265057294465557No Hit
GAATGTGCAACATGCTTCTTCTTTTGATGGTAGTATTTTACTTGGTTCTA25090.10092095905883261No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)