FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR5665152

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5665152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81497
Total Bases19.8 Mbp
Sequences flagged as poor quality0
Sequence length75-252
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTAAAGAACATTGTGTTGTTATGTGTATTTATCATGCTGTTCCTTTG11911.4614034872449293No Hit
GTGTTGGTGCCGGTAACGGTTCTTTCACTTACACCACTAAAGAACATTGT9311.1423733388959103No Hit
CCACTAAAGAACATTGTGTTGTTATGTGTGTTTATCATGCTGTTCCTTTG8621.057707645680209No Hit
CATTGTGTTGTTATGTGTGTTTATCATGCTGTTCCTTTGCTTGACTACAC6260.7681264341018688No Hit
GTGTTGTTATGTGTATTTATCATGCTGTTCCTTTGCTTGACTACACAATT5220.6405143747622611No Hit
ACATTGTGTTGTTATGTGTGTTTATCATGCTGTTCCTTTGCTTGACTACA4180.5129023154226536No Hit
GTGTTGTTATGTGTGTTTATCATGCTGTTCCTTTGCTTGACTACACAATT3460.42455550511061757No Hit
TGACTACACAATTACCGGTCAGGATGGACAGCTGCTTGTTACTGATGCCG1300.1595150741745095No Hit
GGTGATACTGCTGTTATTGCTGGTAAAGGTGTTGGTGCCGGTAACGGTTC1150.14110948869283532No Hit
GAGTTGGTGCCGGTAACGGTTCTTTCACTTACACCACTAAAGAACATTGT1100.13497429353227727No Hit
ACATTGTGTTGTTATGTGTATTTATCATGCTGTTCCTTTGCTTGACTACA1040.1276120593396076No Hit
CATTGTGTTGTTATGTGTATTTATCATGCTGTTCCTTTGCTTGACTACAC1030.126385020307496No Hit
CAATTACCGGTCAGGATGGACAGCTGCTTGTTACTGATGCCGAGCCCACG890.10920647385793342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)