##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665153
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5194391
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.426134844296474	32.0	25.0	33.0	18.0	33.0
2	28.281659582422655	31.0	27.0	33.0	18.0	33.0
3	30.01296205849733	31.0	29.0	33.0	25.0	33.0
4	30.506669020487674	31.0	30.0	33.0	27.0	33.0
5	31.633145444769173	33.0	32.0	33.0	29.0	33.0
6	34.86701001137573	37.0	34.0	38.0	29.0	38.0
7	36.11006352814026	38.0	37.0	38.0	33.0	38.0
8	36.517803338254666	38.0	37.0	38.0	34.0	38.0
9	36.6592707403043	38.0	37.0	38.0	34.0	38.0
10-14	36.76740938446875	38.0	38.0	38.0	34.0	38.0
15-19	36.75638256727304	38.0	38.0	38.0	34.4	38.0
20-24	36.7539460545038	38.0	38.0	38.0	34.6	38.0
25-29	36.699794412858026	38.0	38.0	38.0	34.0	38.0
30-34	36.66656865068494	38.0	38.0	38.0	34.0	38.0
35-39	36.61241365927209	38.0	38.0	38.0	34.0	38.0
40-44	36.63054729611228	38.0	38.0	38.0	34.0	38.0
45-49	36.58045541816163	38.0	38.0	38.0	34.0	38.0
50-54	36.47103054044256	38.0	38.0	38.0	34.0	38.0
55-59	36.35995507461799	38.0	37.2	38.0	33.2	38.0
60-64	36.341255827680286	38.0	37.0	38.0	33.4	38.0
65-69	36.28130881945545	38.0	37.0	38.0	33.0	38.0
70-74	36.20321935718739	38.0	37.0	38.0	33.0	38.0
75-79	36.11871591009283	38.0	37.0	38.0	33.0	38.0
80-84	36.015522716091084	38.0	37.0	38.0	32.0	38.0
85-89	35.89832829176275	38.0	37.0	38.0	31.0	38.0
90-94	35.80385503563973	38.0	37.0	38.0	31.0	38.0
95-99	35.69723723526822	38.0	36.4	38.0	31.0	38.0
100-104	35.57497445169778	38.0	36.0	38.0	29.6	38.0
105-109	35.41629514485381	38.0	36.0	38.0	29.0	38.0
110-114	35.298954911179365	38.0	35.2	38.0	28.6	38.0
115-119	35.160859315585114	38.0	35.0	38.0	28.0	38.0
120-124	35.29200199883236	38.0	35.0	38.0	29.4	38.0
125-129	33.512064047351096	34.4	33.4	35.6	31.0	35.6
130-134	34.926914591648824	36.2	35.4	36.8	32.2	37.2
135-139	36.19639789290662	38.0	37.0	38.0	33.0	38.0
140-144	36.1175481200992	38.0	37.0	38.0	32.4	38.0
145-149	36.0999176031677	38.0	37.0	38.0	32.6	38.0
150-154	36.08446477227172	38.0	37.0	38.0	32.8	38.0
155-159	36.086652427800786	38.0	37.0	38.0	32.6	38.0
160-164	36.053453076421775	38.0	37.0	38.0	31.8	38.0
165-169	36.02896927099212	38.0	37.0	38.0	31.2	38.0
170-174	36.000724264148886	38.0	37.0	38.0	31.0	38.0
175-179	35.96172366006275	38.0	37.0	38.0	31.0	38.0
180-184	35.86581823671012	38.0	37.0	38.0	31.0	38.0
185-189	35.803583230371736	38.0	37.0	38.0	31.0	38.0
190-194	35.74718216176933	38.0	37.0	38.0	29.4	38.0
195-199	35.680945840792454	38.0	37.0	38.0	29.0	38.0
200-204	35.59166950977494	38.0	37.0	38.0	29.0	38.0
205-209	35.5131264594322	38.0	36.4	38.0	29.0	38.0
210-214	35.41362654956016	38.0	36.0	38.0	28.8	38.0
215-219	35.23700495363064	38.0	36.0	38.0	28.0	38.0
220-224	35.10763002839104	38.0	35.8	38.0	27.2	38.0
225-229	34.924094441620866	38.0	35.0	38.0	26.2	38.0
230-234	34.795487139189405	38.0	35.0	38.0	25.2	38.0
235-239	34.66465565482982	38.0	35.0	38.0	24.8	38.0
240-244	34.60682975856247	38.0	34.0	38.0	25.0	38.0
245-249	34.833237931632624	38.0	34.2	38.0	26.6	38.0
250-252	35.861024828641185	37.666666666666664	34.666666666666664	38.0	31.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	5.0
17	18.0
18	32.0
19	51.0
20	237.0
21	1060.0
22	3345.0
23	7933.0
24	15230.0
25	25172.0
26	38483.0
27	54237.0
28	71859.0
29	90945.0
30	115248.0
31	144711.0
32	182457.0
33	238724.0
34	327474.0
35	500259.0
36	1011106.0
37	2365805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.87955110504024	27.013565619248947	13.251407421195108	22.855475854515703
2	13.999993454465509	23.949956693588707	41.70920461937621	20.340845232569578
3	15.838339221424375	32.246255233778484	31.647993611560793	20.267411933236342
4	11.793142708190752	20.223479862767146	44.13034233324728	23.853035095794823
5	11.679960511198248	39.717972333196776	37.6438091600467	10.958257995558279
6	23.771006071741617	45.15603465353301	20.447247810186024	10.625711464539346
7	21.187781433431066	33.52763269604323	30.079682118593325	15.204903751932372
8	20.03473363277182	40.469726083279475	22.784508437854768	16.71103184609393
9	21.844793740016875	17.67131122782247	24.901629469171652	35.582265562989
10-14	20.893362997643113	25.955154466447283	31.897418873152812	21.254063662756796
15-19	20.693117660688912	30.826921994394414	28.471654462348116	20.008305882568557
20-24	20.617031098206866	29.425824968208815	29.365444137740216	20.591699795844104
25-29	20.69638704881619	29.41499786940752	29.311438792353922	20.57717628942236
30-34	20.661037028834457	29.35457404448161	29.343727727172116	20.64066119951183
35-39	20.66627198439424	29.092471546028936	29.337674581642826	20.903581887934003
40-44	20.723028442375067	28.827452014388445	29.51075067441985	20.938768868816638
45-49	20.16613342114147	28.58421387894305	29.955109731425374	21.294542968490102
50-54	20.07979856493495	28.722216093743775	29.83827169977104	21.359713641550236
55-59	19.87862670878523	28.290872664892337	29.771883715220028	22.058616911102412
60-64	19.8865622812019	28.316698132009048	29.37917708965759	22.417562497131463
65-69	19.800821512496476	28.33275133527451	28.952553277272848	22.913873874956167
70-74	20.00695978634372	28.228972734919548	28.379004380113848	23.385063098622876
75-79	20.20647447116303	28.252855328202216	28.011330241743426	23.52933995889133
80-84	20.367160641723487	28.28696692271308	27.59084313574793	23.7550292998155
85-89	20.48703895997484	27.870459985774936	27.73688055291073	23.90562050133949
90-94	20.62186795654217	27.508552503776883	27.523507954131745	24.3460715855492
95-99	20.597358006642104	27.094994826355613	27.687734442794344	24.619912724207943
100-104	20.715314292249655	27.086674148784002	27.611446676821245	24.586564882145097
105-109	20.47030205399303	27.549005008374376	27.48135227070861	24.499340666923985
110-114	20.482737027207826	28.277419163519248	26.932486677611877	24.30735713166105
115-119	20.291855431770347	28.632257626146142	26.875352592426303	24.20053434965721
120-124	20.308589063553338	28.869099535617465	26.846616585101575	23.97569481572762
125-129	20.96853155952269	27.318108197616187	29.954458118136113	21.75890212472501
130-134	18.272251310932035	34.63262803241247	30.43579854377229	16.6593221128832
135-139	21.349564792308843	25.409948768285613	30.972622749519402	22.26786368988615
140-144	20.29852271491957	29.613327774571896	28.972498951136828	21.11565055937171
145-149	20.60179812084155	29.331024232254215	29.45375228990017	20.613425357004065
150-154	20.540511872753513	29.18382428652248	29.507216112955557	20.768447727768446
155-159	20.536299967250333	29.16115649588561	29.46221987656157	20.840323660302488
160-164	20.4644689249561	28.87502595678494	29.595654593644788	21.06485052461417
165-169	20.597034533019883	28.90729707063101	29.332538691272482	21.163129705076624
170-174	20.345157420807727	28.668385873668083	29.594636192100822	21.391820513423372
175-179	20.487515718137445	28.510890362138046	29.50884295457878	21.492750965145728
180-184	20.56223816084398	28.170189035344034	29.521065017448798	21.746507786363185
185-189	20.196278053786447	27.98727019125752	29.83900536002157	21.97744639493446
190-194	20.14613103548969	27.814204094644197	29.888604884027426	22.151059985838685
195-199	20.009664472532094	27.699858253626786	29.921926381677906	22.368550892163213
200-204	20.168764690765038	27.192000810147526	29.69851310060016	22.940721398487277
205-209	20.339397266058608	26.76993634713639	29.726719664563728	23.16394672224127
210-214	20.4351982561712	26.955920919926925	29.37017854929456	23.238702274607313
215-219	20.22699230194634	26.804025247992502	29.180134362160683	23.788848087900476
220-224	20.5603775233781	26.256813139921242	29.758144251499317	23.424665085201337
225-229	20.348062222264428	26.53600772294375	29.488206645644738	23.627723409147087
230-234	20.582766054424674	26.502088230720034	29.402570220805668	23.512575494049628
235-239	20.85791366005588	26.086095508291287	29.572087031176586	23.48390380047625
240-244	20.85842602061317	25.797329403977493	29.497618686851645	23.846625888557693
245-249	20.838474968255774	26.374885397639286	29.299520872798162	23.48711876130678
250-252	19.529379437542044	26.847906484430546	29.865185998169984	23.757528079857423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.3333333333333333
14	0.6666666666666666
15	1.9999999999999998
16	4.999999999999999
17	20.83333333333334
18	56.83333333333342
19	125.16666666666603
20	289.50000000000136
21	593.3333333333295
22	1093.8333333333678
23	1855.3333333331996
24	3114.666666666936
25	5028.999999999964
26	7831.333333330766
27	12493.166666671214
28	19844.33333333361
29	31349.999999958778
30	47588.166666722165
31	66825.33333351735
32	86464.16666656661
33	103558.16666632125
34	116024.33333280744
35	123399.83333270194
36	128694.99999929282
37	130384.33333260364
38	127382.83333265121
39	122913.16666604883
40	117079.33333280047
41	108634.83333292422
42	100471.66666637501
43	93979.33333313222
44	87002.16666656424
45	80910.8333333195
46	77546.66666670331
47	77369.83333337281
48	78136.83333335775
49	78648.66666668121
50	78300.83333335193
51	76841.50000003891
52	71870.66666677852
53	63292.33333350042
54	54524.83333343777
55	45418.66666670559
56	37089.666666644756
57	30239.6666666315
58	24591.83333331865
59	20251.333333333438
60	17401.166666676894
61	14506.83333334098
62	11449.166666669189
63	8919.666666664747
64	6752.833333331769
65	5073.833333333254
66	3765.833333333909
67	2796.0000000001387
68	1992.6666666664994
69	1512.1666666666076
70	1117.333333333363
71	806.1666666666869
72	602.9999999999977
73	425.16666666666043
74	291.0000000000015
75	183.33333333333405
76	104.99999999999962
77	64.66666666666683
78	36.33333333333331
79	20.999999999999993
80	10.166666666666666
81	4.5
82	2.5
83	1.1666666666666665
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08817203017639604
2	1.9251534973012234E-4
3	1.5401227978409788E-4
4	1.5401227978409788E-4
5	1.3476074481108565E-4
6	0.0
7	1.9251534973012235E-5
8	3.850306994602447E-5
9	0.0
10-14	1.5401227978409788E-5
15-19	3.850306994602447E-6
20-24	5.236417512659329E-4
25-29	0.0011781939403483488
30-34	2.3101841967614682E-5
35-39	3.4652762951422024E-5
40-44	1.1550920983807341E-5
45-49	6.892049520338381E-4
50-54	0.0011935951683267588
55-59	0.0019559559532580434
60-64	0.00201371055817708
65-69	0.0041506309401814385
70-74	0.010653799454064972
75-79	0.012082303356758807
80-84	0.003130339360744659
85-89	6.39168803325834E-4
90-94	0.0011974998359694758
95-99	2.5798844866347547E-4
100-104	0.002714744993028304
105-109	4.7751021246085235E-4
110-114	4.8525338082962613E-4
115-119	6.162986811439335E-4
120-124	0.0017724945452443682
125-129	0.029275598126950176
130-134	0.022341024350901885
135-139	0.010241414184125885
140-144	0.008394856172139552
145-149	0.007770285079850056
150-154	0.009492708052365144
155-159	0.012972477593090233
160-164	0.015509586111293655
165-169	0.012464357968602154
170-174	0.01031526418966617
175-179	0.013690447233431784
180-184	0.0061295649595582554
185-189	0.006932822655486783
190-194	0.006106785643715149
195-199	0.004889038802166869
200-204	0.0033806757314835227
205-209	2.91305939285317E-4
210-214	8.903512941294941E-4
215-219	7.322856688461644E-4
220-224	0.0010313288402554837
225-229	6.474723933467777E-4
230-234	3.3627746229676874E-4
235-239	3.606428964529329E-5
240-244	0.0
245-249	1.2900221858015513E-5
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	43.0
80-84	62.0
85-89	100.0
90-94	96.0
95-99	148.0
100-104	206.0
105-109	346.0
110-114	656.0
115-119	1194.0
120-124	4861.0
125-129	31141.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	5.0
155-159	6.0
160-164	15.0
165-169	32.0
170-174	36.0
175-179	62.0
180-184	116.0
185-189	217.0
190-194	390.0
195-199	825.0
200-204	2952.0
205-209	4387.0
210-214	7136.0
215-219	12803.0
220-224	18610.0
225-229	32182.0
230-234	55532.0
235-239	79834.0
240-244	148167.0
245-249	567432.0
250-253	4224799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.301674560589724
#Duplication Level	Percentage of total
1	13.223220531768881
2	6.936666365646295
3	4.350725828257791
4	3.2299413333750886
5	2.504173691264653
6	2.2108893062172523
7	1.959899514880321
8	1.8714178025911694
9	1.684541323476461
>10	30.736768620532047
>50	13.21578851189945
>100	15.402935862155687
>500	1.6980223262260281
>1k	0.9750089817089689
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.9251534973012235E-5	0.0	0.0
3	0.0	0.0	0.0	1.9251534973012235E-5	0.0	0.0
4	0.0	0.0	0.0	1.9251534973012235E-5	0.0	0.0
5	0.0	0.0	0.0	3.850306994602447E-5	0.0	0.0
6	0.0	0.0	0.0	3.850306994602447E-5	0.0	0.0
7	0.0	0.0	0.0	3.850306994602447E-5	0.0	0.0
8	0.0	0.0	0.0	5.7754604919036706E-5	0.0	0.0
9	0.0	0.0	0.0	5.7754604919036706E-5	0.0	0.0
10-14	0.0	0.0	0.0	5.77546049190367E-5	3.0802455956819577E-5	0.0
15-19	0.0	0.0	0.0	5.77546049190367E-5	1.2706013082188075E-4	3.0802455956819577E-5
20-24	0.0	0.0	0.0	2.3871903366535176E-4	1.8096442874631502E-4	3.850306994602447E-5
25-29	0.0	0.0	0.0	0.008127998065605765	2.0791657770853216E-4	3.850306994602447E-5
30-34	0.0	0.0	0.0	0.22094216627127222	4.427853043792814E-4	3.850306994602447E-5
35-39	0.0	0.0	0.0	0.8741428975985828	5.313423652551377E-4	3.850306994602447E-5
40-44	0.0	0.0	0.0	2.322663041730975	5.967975841633793E-4	3.850306994602447E-5
45-49	0.0	0.0	1.1550920983807341E-5	4.03089409326329	6.507018820878137E-4	4.6203683935229365E-5
50-54	0.0	0.0	1.9251534973012235E-5	6.014218028638968	7.007558730176453E-4	5.77546049190367E-5
55-59	0.0	0.0	1.9251534973012235E-5	8.119816163242234	7.893129338935016E-4	6.930552590284405E-5
60-64	0.0	0.0	1.9251534973012235E-5	10.422596219653084	0.0012975534571810245	9.625767486506118E-5
65-69	0.0	0.0	1.9251534973012235E-5	12.79415816021551	0.0024526455555617587	9.625767486506118E-5
70-74	0.0	0.0	1.9251534973012235E-5	15.344647717124104	0.0026374602913026768	1.0780859584886852E-4
75-79	0.0	1.5401227978409788E-5	2.6952148962217126E-5	17.927714721514032	0.0029108320879194503	1.155092098380734E-4
80-84	0.0	1.9251534973012235E-5	3.850306994602447E-5	20.605961314810532	0.0033497670853041292	1.155092098380734E-4
85-89	0.0	1.9251534973012235E-5	3.850306994602447E-5	23.304117845576123	0.003961965897445918	1.155092098380734E-4
90-94	0.0	1.9251534973012235E-5	3.850306994602447E-5	26.044562298063433	0.00497074633003176	1.155092098380734E-4
95-99	0.0	1.9251534973012235E-5	3.850306994602447E-5	28.810230111672382	0.02915067425613512	1.3091043781648321E-4
100-104	0.0	1.9251534973012235E-5	3.850306994602447E-5	31.53995145917972	0.11497401716582367	1.3476074481108565E-4
105-109	0.0	1.9251534973012235E-5	3.850306994602447E-5	34.23434624001158	0.3134034384396554	1.3476074481108565E-4
110-114	0.0	1.9251534973012235E-5	3.850306994602447E-5	36.8516578748115	0.5252280777477091	1.3476074481108565E-4
115-119	0.0	1.9251534973012235E-5	3.850306994602447E-5	38.43768788294913	0.6978835439996718	1.46311665794893E-4
120-124	0.0	1.9251534973012235E-5	3.850306994602447E-5	38.48488109578197	0.7138199646503315	1.7711412175171255E-4
125-129	0.0	1.9251534973012235E-5	3.850306994602447E-5	38.485462492138154	0.7138469167992937	1.9251534973012237E-4
130-134	0.0	3.850306994602447E-5	3.850306994602447E-5	38.485809019767665	0.7139085217112073	2.0406627071392971E-4
135-139	0.0	3.850306994602447E-5	3.850306994602447E-5	38.4869333094101	0.7139431744741588	2.1176688470313457E-4
140-144	0.0	3.850306994602447E-5	3.850306994602447E-5	38.48908178071308	0.7139701266231209	2.2716811268154438E-4
145-149	0.0	3.850306994602447E-5	3.850306994602447E-5	38.49211967293182	0.7140779352189699	2.387190336653517E-4
150-154	0.0	3.850306994602447E-5	3.850306994602447E-5	38.4964782204497	0.7141125879819212	2.57970568638364E-4
155-159	0.0	3.850306994602447E-5	3.850306994602447E-5	38.5029813889636	0.714181893507824	3.0802455956819577E-4
160-164	0.0	3.850306994602447E-5	3.850306994602447E-5	38.512460844784314	0.7142781511826891	3.349767085304129E-4
165-169	0.0	3.850306994602447E-5	3.850306994602447E-5	38.525890715581475	0.7143320554806136	3.657791644872325E-4
170-174	0.0	3.850306994602447E-5	3.850306994602447E-5	38.541958046669954	0.7143590076295758	3.657791644872325E-4
175-179	0.0	3.850306994602447E-5	5.005399092983181E-5	38.56399335360006	0.7144629659184301	3.657791644872325E-4
180-184	0.0	3.850306994602447E-5	5.77546049190367E-5	38.59306317140932	0.7145977266632413	3.657791644872325E-4
185-189	0.0	3.850306994602447E-5	5.77546049190367E-5	38.62402734025991	0.714763289864009	4.158331554170643E-4
190-194	0.0	3.850306994602447E-5	5.77546049190367E-5	38.6568473570819	0.7148556972318796	4.4278530437928145E-4
195-199	0.0	3.850306994602447E-5	5.77546049190367E-5	38.69247809800995	0.7150982665725396	4.5433622536308877E-4
200-204	0.0	3.850306994602447E-5	5.77546049190367E-5	38.728251300296805	0.7163881194157313	4.620368393522936E-4
205-209	0.0	3.850306994602447E-5	5.77546049190367E-5	38.76592270393199	0.7225871136770412	4.620368393522936E-4
210-214	0.0	3.850306994602447E-5	5.77546049190367E-5	38.80025974170986	0.7325093548021318	4.620368393522936E-4
215-219	0.0	3.850306994602447E-5	5.77546049190367E-5	38.834750791767505	0.745658153188699	4.851386813199083E-4
220-224	0.0	3.850306994602447E-5	5.77546049190367E-5	38.86349718378921	0.7666846796862231	5.120908302821255E-4
225-229	0.0	3.850306994602447E-5	5.77546049190367E-5	38.89716426815001	0.790133049283352	5.274920582605352E-4
230-234	0.0	3.850306994602447E-5	5.77546049190367E-5	38.93592915897167	0.8077751559326204	5.390429792443426E-4
235-239	0.0	3.850306994602447E-5	5.77546049190367E-5	38.98207893860897	0.8179130142494087	5.390429792443426E-4
240-241	0.0	3.850306994602447E-5	5.7754604919036706E-5	39.049707655815666	0.8185175124475612	5.390429792443426E-4
>>END_MODULE
