##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665154
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3227849
Total Bases	802.9 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.849582802665182	32.0	25.0	33.0	18.0	33.0
2	27.724593684524894	29.0	25.0	33.0	18.0	33.0
3	29.66423212486086	31.0	29.0	33.0	25.0	33.0
4	30.13932622003074	31.0	29.0	33.0	27.0	33.0
5	31.50271124826471	33.0	32.0	33.0	29.0	33.0
6	34.80404907416673	37.0	34.0	38.0	29.0	38.0
7	36.11521046988258	38.0	36.0	38.0	33.0	38.0
8	36.50234072287768	38.0	37.0	38.0	34.0	38.0
9	36.59678690050247	38.0	37.0	38.0	34.0	38.0
10-14	36.72231489143389	38.0	38.0	38.0	34.0	38.0
15-19	36.70563269843168	38.0	38.0	38.0	34.0	38.0
20-24	36.709914559200264	38.0	38.0	38.0	34.0	38.0
25-29	36.64946346622782	38.0	38.0	38.0	34.0	38.0
30-34	36.61683839609598	38.0	38.0	38.0	34.0	38.0
35-39	36.55755222750506	38.0	38.0	38.0	34.0	38.0
40-44	36.577806954414534	38.0	38.0	38.0	34.0	38.0
45-49	36.52347708954168	38.0	38.0	38.0	34.0	38.0
50-54	36.402920830559296	38.0	37.6	38.0	33.8	38.0
55-59	36.287327938822415	38.0	37.0	38.0	33.2	38.0
60-64	36.264925279961986	38.0	37.0	38.0	33.0	38.0
65-69	36.20174128343674	38.0	37.0	38.0	33.0	38.0
70-74	36.1240144752744	38.0	37.0	38.0	33.0	38.0
75-79	36.05353869456171	38.0	37.0	38.0	32.6	38.0
80-84	35.97498699124428	38.0	37.0	38.0	31.0	38.0
85-89	35.872803497954706	38.0	37.0	38.0	31.0	38.0
90-94	35.79353973553976	38.0	37.0	38.0	31.0	38.0
95-99	35.70371148873194	38.0	36.0	38.0	31.0	38.0
100-104	35.58812657668517	38.0	36.0	38.0	30.0	38.0
105-109	35.4306008103094	38.0	36.0	38.0	29.0	38.0
110-114	35.31255561575398	38.0	35.6	38.0	29.0	38.0
115-119	35.18842412672629	38.0	35.0	38.0	28.4	38.0
120-124	35.30979029719477	38.0	35.0	38.0	29.4	38.0
125-129	33.639655230698466	34.4	33.4	35.6	31.2	35.6
130-134	35.236961619307905	36.2	36.0	37.0	33.0	37.2
135-139	36.514218896515985	38.0	38.0	38.0	34.0	38.0
140-144	36.42981523885116	38.0	38.0	38.0	34.0	38.0
145-149	36.42220363788417	38.0	38.0	38.0	34.0	38.0
150-154	36.40493172414698	38.0	38.0	38.0	34.0	38.0
155-159	36.398114316323074	38.0	38.0	38.0	34.0	38.0
160-164	36.36494934116128	38.0	38.0	38.0	33.8	38.0
165-169	36.338413767876304	38.0	38.0	38.0	33.4	38.0
170-174	36.31408897166226	38.0	38.0	38.0	33.0	38.0
175-179	36.27615462386423	38.0	37.8	38.0	33.0	38.0
180-184	36.190644416548515	38.0	37.0	38.0	33.0	38.0
185-189	36.14050865403155	38.0	37.0	38.0	33.0	38.0
190-194	36.098775846375354	38.0	37.0	38.0	33.0	38.0
195-199	36.04480109567902	38.0	37.0	38.0	32.4	38.0
200-204	35.96545831183705	38.0	37.0	38.0	31.0	38.0
205-209	35.90467747291222	38.0	37.0	38.0	31.0	38.0
210-214	35.8274627566448	38.0	37.0	38.0	31.0	38.0
215-219	35.6890993824173	38.0	37.0	38.0	30.6	38.0
220-224	35.58366916590472	38.0	36.2	38.0	29.0	38.0
225-229	35.432265392946384	38.0	36.0	38.0	29.0	38.0
230-234	35.31969839200463	38.0	36.0	38.0	28.6	38.0
235-239	35.213509001116485	38.0	36.0	38.0	28.2	38.0
240-244	35.152321380572445	38.0	35.2	38.0	28.0	38.0
245-249	35.30665861564252	38.0	35.4	38.0	29.2	38.0
250-252	36.10207751160365	37.666666666666664	35.333333333333336	38.0	31.666666666666668	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	11.0
17	33.0
18	54.0
19	84.0
20	154.0
21	470.0
22	1385.0
23	3352.0
24	6781.0
25	11654.0
26	18955.0
27	28257.0
28	38309.0
29	49141.0
30	62696.0
31	80038.0
32	103878.0
33	139108.0
34	196787.0
35	310646.0
36	654157.0
37	1521896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.416025021152215	27.493845008620553	12.571157156981503	19.518972813245732
2	17.924516611128944	25.675932730163492	40.186304384733305	16.213246273974267
3	14.235375432293864	36.28037210034329	34.44000769555111	15.04424477181173
4	10.76583054560911	23.616800326657874	46.18206219511364	19.43530693261938
5	10.193153756239074	40.92181682938111	38.9489203780977	9.936109036282124
6	19.415269501704852	48.064746583325224	23.039234213776	9.480749701193924
7	17.232575531794268	35.73946836373235	32.9551347864799	14.072821317993487
8	16.78712898586734	38.6986413833143	23.459667480626376	21.054562150191984
9	19.235236366531623	17.245953727050882	24.66922998518827	38.84957992122923
10-14	21.18022490417603	25.470027099807556	31.932900883926983	21.41684711208943
15-19	20.831276234209906	30.57638678222111	28.267410741725506	20.324926241843478
20-24	20.550754844165052	29.51907841046736	29.452965919723574	20.47720082564401
25-29	20.764742570796177	29.538416011125296	29.05201895672107	20.64482246135746
30-34	20.225027025784463	29.533557916719253	29.647962475502993	20.59345258199329
35-39	20.71457241608608	29.270228582326602	29.289727668341797	20.725471333245515
40-44	20.663376503735837	28.955449584586518	29.774919124039506	20.60625478763814
45-49	19.890312707240586	29.147626272580723	29.91187331825902	21.050187701919672
50-54	19.989053904408973	29.028715872916734	29.950254933859956	21.031975288814337
55-59	19.98398787137952	28.567906494423205	29.711483029696485	21.736622604500795
60-64	19.672116825226873	28.848551767212054	29.673325080023645	21.80600632753743
65-69	19.583669378324615	28.622916128622904	29.3437171442182	22.44969734883428
70-74	19.527066555598456	28.388511442460885	29.14756636130345	22.93685564063721
75-79	19.25804606568968	28.30884052501675	29.149397765066098	23.283715644227474
80-84	18.943662783470952	28.071238174032587	29.174914579649837	23.810184462846625
85-89	18.980461315337696	27.594733791114567	29.2942086635971	24.13059622995064
90-94	18.789730766504178	27.39855099047131	29.43238327340617	24.37933496961834
95-99	18.5803420806608	27.06049739407072	29.47644934249723	24.88271118277126
100-104	18.834117763645093	26.91743646566894	29.48128191218748	24.767163858498495
105-109	18.32079350190592	27.326037807043235	29.419179601615276	24.933989089435567
110-114	18.198972508678185	27.934763022486454	28.9947411749224	24.87152329391296
115-119	18.155692707952625	27.95316755885163	28.960824149386376	24.93031558380937
120-124	18.139046987094883	28.354478806859763	28.928488956612625	24.57798524943273
125-129	21.267350026989536	28.13752832028293	31.159775879126833	19.435345773600705
130-134	15.558688773382787	35.8791952527576	32.10242533598961	16.459690637870004
135-139	21.10142674595899	24.875309659989387	31.073079381884483	22.950184212167144
140-144	20.65027343408141	29.53745387728764	28.405925442183953	21.406347246446998
145-149	20.60944113424671	29.398975471525553	29.45737525973633	20.534208134491415
150-154	20.610397097350067	29.543098315503734	29.170332237329532	20.676172349816667
155-159	20.125139659488458	29.378215016671493	29.58082965639775	20.9158156674423
160-164	20.65011504200511	29.049859215029883	29.464770451884192	20.835255291080816
165-169	20.521027182290336	29.068882695043225	29.620688234721847	20.789401887944592
170-174	20.02559939661248	29.26979743222251	29.52077068131631	21.183832489848697
175-179	20.423789073460753	28.758959861598022	29.71334703379702	21.103904031144204
180-184	20.41654884302874	28.612084601639452	29.503898681666957	21.467467873664848
185-189	19.96303396916918	28.578449328659545	29.926570660330658	21.53194604184062
190-194	19.87948793736774	28.377470982184644	30.024712091394477	21.71832898905314
195-199	19.822741738210176	28.250846193466206	30.08828412915613	21.83812793916749
200-204	19.920124889659167	27.987496000418936	29.703801738041914	22.388577371879983
205-209	20.07129167874392	27.472845855648426	30.03859014946449	22.417272316143162
210-214	20.062218120431304	27.553348109153607	29.719985820032917	22.664447950382165
215-219	19.951996787881672	27.57632632074065	29.639374202794144	22.832302688583532
220-224	19.989873725406245	27.041250574740467	30.271641854646354	22.69723384520693
225-229	19.965294058315955	27.31507829148663	29.957135175365362	22.762492474832055
230-234	20.069014202456735	27.328535178806774	29.750521650443734	22.85192896829276
235-239	20.346271471167086	26.94957428375512	29.987560237508948	22.716594007568848
240-244	20.237815015939596	26.77449465763933	29.89690458760658	23.090785738814493
245-249	20.307831289363115	27.321625744650024	29.545326708540358	22.825216257446503
250-252	19.292281212197732	27.444651059446013	30.321452919166703	22.941614809189552
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.3333333333333333
10	0.3333333333333333
11	0.8333333333333333
12	2.833333333333333
13	4.999999999999999
14	10.666666666666668
15	24.333333333333314
16	46.00000000000005
17	87.16666666666652
18	124.166666666666
19	160.33333333333348
20	254.0000000000028
21	360.99999999999704
22	493.99999999998965
23	820.5000000000223
24	1329.6666666666486
25	2144.3333333331716
26	3434.1666666670644
27	5449.499999999669
28	8554.166666663936
29	13954.000000006916
30	22036.499999992586
31	31848.166666624125
32	43855.166666693214
33	56721.666666786026
34	71897.83333344648
35	88487.16666654112
36	105367.99999963096
37	115486.66666615198
38	118693.33333277414
39	117365.49999946411
40	110219.16666624234
41	99923.33333306089
42	86172.33333325373
43	71459.00000012529
44	57771.50000012574
45	46356.83333337576
46	37742.33333331075
47	31041.333333298022
48	26092.33333331465
49	22586.999999993113
50	19750.500000002507
51	17639.0000000097
52	16102.166666677007
53	14495.166666674375
54	13453.666666672692
55	12540.00000000451
56	11707.333333336423
57	11097.000000002026
58	10619.33333333454
59	10197.833333333789
60	9752.999999999647
61	9164.833333331937
62	8742.499999997872
63	8318.999999997119
64	7940.83333333065
65	7424.833333331115
66	6776.833333331698
67	6229.3333333321925
68	5664.16666666603
69	4974.833333333315
70	4270.500000000618
71	3622.166666667182
72	3005.8333333335704
73	2443.1666666666524
74	1900.9999999998497
75	1449.4999999999518
76	1008.0000000000437
77	694.0000000000083
78	474.49999999999073
79	305.0000000000004
80	178.8333333333339
81	79.0
82	38.16666666666664
83	17.333333333333343
84	9.666666666666664
85	2.333333333333333
86	0.3333333333333333
87	0.3333333333333333
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07491056737784202
2	4.0274498590237647E-4
3	3.098038353095203E-4
4	2.1686268471666427E-4
5	1.8588230118571223E-4
6	9.294115059285611E-5
7	9.294115059285611E-5
8	2.1686268471666427E-4
9	6.196076706190408E-5
10-14	9.913722729904652E-5
15-19	8.05489971804753E-5
20-24	5.32862596732375E-4
25-29	0.0010347448099337982
30-34	1.734901477733314E-4
35-39	1.6729407106714098E-4
40-44	1.4870584094856977E-4
45-49	5.700390569695175E-4
50-54	9.727840428718939E-4
55-59	0.0014189015657176034
60-64	0.0016419603271404578
65-69	0.003302508884399487
70-74	0.008761252462553236
75-79	0.01049625864764072
80-84	0.0020571595410254
85-89	3.4080966461850386E-4
90-94	8.799374872579474E-4
95-99	1.4253237266514157E-4
100-104	0.0019211939861174523
105-109	2.7890546833837234E-4
110-114	4.215026084502967E-4
115-119	6.819754447921348E-4
120-124	0.001321128832017911
125-129	0.025568740616549977
130-134	0.01877908410650996
135-139	0.007812098988308142
140-144	0.006172058976339607
145-149	0.0053019614127379775
150-154	0.007017120396634436
155-159	0.00957734308603152
160-164	0.011736963566774967
165-169	0.009702592382450902
170-174	0.007411581522576716
175-179	0.009959452944965166
180-184	0.004306885363795032
185-189	0.004920548479641293
190-194	0.004150752838883301
195-199	0.003418593643168949
200-204	0.002323899907438628
205-209	1.755548080543292E-4
210-214	5.523327774857109E-4
215-219	4.464049937000309E-4
220-224	6.240630780266066E-4
225-229	4.112961149158814E-4
230-234	2.2926138160168496E-4
235-239	1.2866278006429408E-5
240-244	0.0
245-249	6.817968291811225E-6
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	65.0
80-84	140.0
85-89	99.0
90-94	123.0
95-99	178.0
100-104	250.0
105-109	259.0
110-114	511.0
115-119	811.0
120-124	4158.0
125-129	26211.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	3.0
155-159	5.0
160-164	8.0
165-169	15.0
170-174	41.0
175-179	50.0
180-184	105.0
185-189	128.0
190-194	247.0
195-199	465.0
200-204	2625.0
205-209	3267.0
210-214	4420.0
215-219	7130.0
220-224	10250.0
225-229	15710.0
230-234	27377.0
235-239	39287.0
240-244	75271.0
245-249	314944.0
250-253	2693696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.604681573973025
#Duplication Level	Percentage of total
1	25.56588949988978
2	7.764859210017002
3	4.369058190314101
4	3.15780266338809
5	2.616123252576217
6	2.1952655091536553
7	1.8418728876033228
8	1.7524535708331936
9	1.5054558280126196
>10	22.949822556787392
>50	8.270789045248923
>100	14.188450768272476
>500	2.403191233630157
>1k	1.4189657842730437
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.098038353095204E-5	0.0	0.0
5	0.0	0.0	0.0	3.098038353095204E-5	0.0	0.0
6	0.0	0.0	0.0	3.098038353095204E-5	3.098038353095204E-5	0.0
7	0.0	0.0	0.0	3.098038353095204E-5	3.098038353095204E-5	0.0
8	0.0	0.0	0.0	3.098038353095204E-5	3.098038353095204E-5	0.0
9	0.0	0.0	0.0	3.098038353095204E-5	3.098038353095204E-5	0.0
10-14	0.0	0.0	0.0	3.098038353095204E-5	1.3011761082999855E-4	0.0
15-19	0.0	0.0	0.0	8.67450738866657E-5	3.779606790776149E-4	3.098038353095204E-5
20-24	0.0	0.0	0.0	8.798428922790378E-4	7.869017416861817E-4	6.196076706190408E-5
25-29	0.0	0.0	0.0	0.011747761434937012	9.232154292223707E-4	6.196076706190408E-5
30-34	0.0	0.0	0.0	0.18598763448971747	0.0015118427163104593	7.43529204742849E-5
35-39	0.0	0.0	2.478430682476163E-5	0.6991714916032318	0.001641960327140458	1.2392153412380815E-4
40-44	0.0	0.0	5.5764690355713666E-5	1.8025006745978516	0.001722509324320933	1.2392153412380815E-4
45-49	0.0	0.0	8.67450738866657E-5	3.15680814065342	0.0018898033953880743	1.2392153412380815E-4
50-54	0.0	0.0	2.0447053130428347E-4	4.7801926298287185	0.0021314503869295	1.4870584094856977E-4
55-59	0.0	6.196076706190408E-6	2.1686268471666427E-4	6.486988703622754	0.0023359209182337837	1.7968622447952186E-4
60-64	0.0	3.098038353095204E-5	2.1686268471666427E-4	8.375664413050298	0.0029679207422652052	2.1066660801047384E-4
65-69	0.0	3.098038353095204E-5	2.1686268471666427E-4	10.362182369745302	0.004975449595070897	3.098038353095204E-4
70-74	0.0	3.098038353095204E-5	2.1686268471666427E-4	12.495547344377014	0.005341018120736131	3.098038353095204E-4
75-79	0.0	3.098038353095204E-5	2.1686268471666427E-4	14.662581799830164	0.0058552924873499345	3.407842188404724E-4
80-84	0.0	3.098038353095204E-5	2.1686268471666427E-4	16.975670175401635	0.006474900157968975	3.7176460237142446E-4
85-89	0.0	3.098038353095204E-5	2.1686268471666427E-4	19.33833955677604	0.007875213493568008	4.461175228457093E-4
90-94	0.0	3.098038353095204E-5	2.1686268471666427E-4	21.726183597807704	0.009833173732724177	4.647057529642806E-4
95-99	0.0	3.098038353095204E-5	2.1686268471666427E-4	24.13735586763817	0.027900933407975403	4.70901829670471E-4
100-104	0.0	3.098038353095204E-5	2.1686268471666427E-4	26.582556990739032	0.08394444721546764	5.01882213201423E-4
105-109	0.0	3.098038353095204E-5	2.1686268471666427E-4	28.978796715707585	0.21149068621239717	5.266665200261846E-4
110-114	0.0	3.098038353095204E-5	2.1686268471666427E-4	31.3374758236832	0.34844876572602995	5.266665200261846E-4
115-119	0.0	3.098038353095204E-5	2.1686268471666427E-4	32.78684969464185	0.46539351747866775	5.266665200261846E-4
120-124	0.0	3.098038353095204E-5	2.1686268471666427E-4	32.834547093126105	0.47770512189386805	5.266665200261846E-4
125-129	0.0	3.098038353095204E-5	2.664312983661875E-4	32.83493744595859	0.47774849443081135	5.32862596732375E-4
130-134	0.0	3.098038353095204E-5	2.788234517785683E-4	32.8354517203252	0.4778042591211671	5.576469035571366E-4
135-139	0.0	3.098038353095204E-5	3.34588142134282E-4	32.836337759294196	0.4778910041950537	5.576469035571366E-4
140-144	6.196076706190408E-5	3.098038353095204E-5	3.407842188404724E-4	32.83803548431169	0.4781016708030642	5.762351336757078E-4
145-149	6.196076706190408E-5	3.098038353095204E-5	3.407842188404724E-4	32.840705993372055	0.47830614133436855	6.010194405004695E-4
150-154	6.196076706190408E-5	3.098038353095204E-5	3.407842188404724E-4	32.84449179623954	0.4785230040190852	6.196076706190408E-4
155-159	6.196076706190408E-5	3.098038353095204E-5	3.407842188404724E-4	32.84949822621814	0.4787088863202708	6.629802075623735E-4
160-164	6.196076706190408E-5	5.5764690355713666E-5	3.407842188404724E-4	32.85722473387076	0.4789257490049875	7.125488212118968E-4
165-169	6.196076706190408E-5	6.196076706190408E-5	3.407842188404724E-4	32.86774567211787	0.4790744548459361	7.187448979180872E-4
170-174	6.196076706190408E-5	6.196076706190408E-5	3.407842188404724E-4	32.88122213895384	0.47916119991982276	7.745095882738009E-4
175-179	6.196076706190408E-5	6.196076706190408E-5	3.407842188404724E-4	32.901018604030114	0.479365670451127	7.930978183923722E-4
180-184	6.196076706190408E-5	6.196076706190408E-5	3.407842188404724E-4	32.925926832389	0.4795329645221941	8.240782019233242E-4
185-189	6.196076706190408E-5	6.196076706190408E-5	3.407842188404724E-4	32.953517961961666	0.4796630821330242	8.736468155728474E-4
190-194	6.196076706190408E-5	6.196076706190408E-5	3.407842188404724E-4	32.9834821889128	0.47979939582056036	8.984311223976091E-4
195-199	7.43529204742849E-5	9.294115059285611E-5	3.4698029554666276E-4	33.01283920034673	0.4800906114257513	8.984311223976091E-4
200-204	9.294115059285611E-5	9.294115059285611E-5	3.7176460237142446E-4	33.043423034968484	0.4813670032272266	9.170193525161803E-4
205-209	9.294115059285611E-5	9.294115059285611E-5	3.7176460237142446E-4	33.07778647638102	0.4865717076604265	9.294115059285612E-4
210-214	9.294115059285611E-5	9.294115059285611E-5	3.903528324899957E-4	33.11423799564354	0.49595256779359875	9.479997360471324E-4
215-219	9.294115059285611E-5	9.294115059285611E-5	4.027449859023764E-4	33.14681077088799	0.506925819640262	9.60391889459513E-4
220-224	9.294115059285611E-5	9.294115059285611E-5	4.0894106260856684E-4	33.17420982208275	0.5236366385168575	9.60391889459513E-4
225-229	9.294115059285611E-5	9.294115059285611E-5	4.3372536943332853E-4	33.2058903622815	0.5416052609648097	9.60391889459513E-4
230-234	9.294115059285611E-5	9.294115059285611E-5	4.3372536943332853E-4	33.24516109644534	0.5564386685994296	9.60391889459513E-4
235-239	9.294115059285611E-5	9.294115059285611E-5	4.3372536943332853E-4	33.29251151463406	0.5653114504426942	9.60391889459513E-4
240-241	9.294115059285611E-5	9.294115059285611E-5	4.3372536943332853E-4	33.364215612316436	0.5657327836587152	9.603918894595131E-4
>>END_MODULE
