##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665165
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3403
Total Bases	820.7 kbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.18571848369086	28.0	18.0	32.0	18.0	33.0
2	26.803996473699677	28.0	25.0	31.0	18.0	33.0
3	26.059653247134882	28.0	18.0	31.0	18.0	33.0
4	29.421392888627683	31.0	29.0	33.0	25.0	33.0
5	30.83161915956509	33.0	31.0	33.0	27.0	33.0
6	34.41610343814281	37.0	34.0	38.0	28.0	38.0
7	34.56450191007934	37.0	34.0	38.0	28.0	38.0
8	35.12430208639436	38.0	35.0	38.0	28.0	38.0
9	35.39288862768146	38.0	36.0	38.0	29.0	38.0
10-14	35.49714957390538	38.0	36.0	38.0	29.0	38.0
15-19	35.36273875991772	38.0	36.0	38.0	28.8	38.0
20-24	35.35721422274464	38.0	36.0	38.0	28.2	38.0
25-29	35.13082574199235	38.0	36.0	38.0	27.2	38.0
30-34	35.119365265941816	38.0	36.0	38.0	27.2	38.0
35-39	34.94769321187188	38.0	35.2	38.0	26.8	38.0
40-44	34.898736409050834	38.0	35.2	38.0	27.0	38.0
45-49	34.96855715545107	38.0	35.6	38.0	27.0	38.0
50-54	34.77525712606524	38.0	35.0	38.0	25.0	38.0
55-59	34.590185130766976	38.0	34.4	38.0	25.0	38.0
60-64	34.502086394357924	38.0	34.4	38.0	25.0	38.0
65-69	34.451131354687035	38.0	34.2	38.0	25.0	38.0
70-74	34.227093740816926	38.0	34.0	38.0	23.4	38.0
75-79	34.11715479805401	38.0	34.0	38.0	23.0	38.0
80-84	33.94821232488253	38.0	34.0	38.0	21.4	38.0
85-89	33.66333137139494	38.0	33.6	38.0	15.8	38.0
90-94	33.5569840684643	38.0	33.4	38.0	16.2	38.0
95-99	33.4889654201209	38.0	33.2	38.0	16.6	38.0
100-104	33.352295648714275	38.0	33.2	38.0	15.0	38.0
105-109	33.20841902059438	37.6	33.0	38.0	15.0	38.0
110-114	32.95504607106456	37.4	31.4	38.0	15.0	38.0
115-119	32.918150702140636	37.0	31.4	38.0	15.4	38.0
120-124	33.17240677516075	37.0	31.8	38.0	17.8	38.0
125-129	31.19002617863879	34.0	29.4	35.2	19.0	35.6
130-134	31.597929354445796	35.4	29.0	36.4	15.8	37.2
135-139	32.42856272838002	37.0	29.0	38.0	16.0	38.0
140-144	32.409196102314255	37.0	28.8	38.0	16.0	38.0
145-149	32.33337393422655	37.0	28.2	38.0	16.0	38.0
150-154	32.21071863580998	37.0	28.0	38.0	16.0	38.0
155-159	32.35334957369062	37.0	28.4	38.0	16.0	38.0
160-164	32.22222898903776	37.0	28.2	38.0	16.0	38.0
165-169	32.208160779537145	37.0	28.0	38.0	16.0	38.0
170-174	32.18023693139283	37.0	28.2	38.0	16.0	38.0
175-179	32.154686238579856	37.0	28.0	38.0	16.0	38.0
180-184	32.04762806923586	37.0	28.0	38.0	16.0	38.0
185-189	31.939559063496837	37.0	28.0	38.0	16.0	38.0
190-194	31.816175603251217	37.0	27.4	38.0	15.6	38.0
195-199	31.720107380466807	37.0	27.2	38.0	15.0	38.0
200-204	31.564009229733493	37.0	26.8	38.0	15.0	38.0
205-209	31.325047171679067	36.8	26.0	38.0	15.0	38.0
210-214	31.252867405282938	36.8	25.8	38.0	15.0	38.0
215-219	31.20855449647824	36.0	25.6	38.0	15.0	38.0
220-224	31.13035907871039	36.2	25.4	38.0	15.0	38.0
225-229	31.086234600270416	36.0	25.2	38.0	15.0	38.0
230-234	31.21925239206804	36.0	25.2	38.0	15.0	38.0
235-239	31.39276291135035	36.4	25.6	38.0	15.0	38.0
240-244	31.84911349309835	36.8	28.0	38.0	14.8	38.0
245-249	32.81122477924245	37.4	31.2	38.0	17.2	38.0
250-252	35.251777503593786	37.666666666666664	33.666666666666664	38.0	29.666666666666668	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	9.0
21	20.0
22	35.0
23	53.0
24	90.0
25	107.0
26	139.0
27	162.0
28	180.0
29	170.0
30	162.0
31	164.0
32	168.0
33	189.0
34	236.0
35	283.0
36	439.0
37	792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.62022339800117	19.841269841269842	14.168136390358613	20.37037037037037
2	15.603878930355568	20.981486923302967	40.14105201292977	23.273582133411697
3	17.543344108139877	30.14986776373788	31.736702909197767	20.57008521892448
4	11.960035263003233	20.805171907140757	43.75550984425507	23.479282985600943
5	11.460476050543637	38.55421686746988	38.08404349103732	11.901263590949164
6	22.803408756979135	45.518660005877166	20.59947105495151	11.078460182192183
7	21.569203643843668	33.41169556273876	29.70908022333235	15.31002057008522
8	20.59947105495151	37.466940934469584	24.272700558330882	17.660887452248016
9	20.922715251248896	18.16044666470761	26.594181604466648	34.32265647957685
10-14	21.62209814869233	26.06523655598002	30.390831619159563	21.921833676168088
15-19	20.88157508081105	29.785483397002643	28.36908610049956	20.96385542168675
20-24	21.05201292976785	28.41022627093741	28.956802821040256	21.58095797825448
25-29	21.563326476638263	29.015574493094327	28.686453129591538	20.734645900675876
30-34	20.910960916838086	29.074346165148395	29.039083161915958	20.975609756097562
35-39	20.828680575962384	29.086100499559215	28.733470467234795	21.35174845724361
40-44	20.77578607111372	27.65207170143991	29.385836027034966	22.1863062004114
45-49	20.152806347340583	27.599177196591242	30.59653247134881	21.651483984719366
50-54	19.52982662356744	28.045841904202174	30.12635909491625	22.297972377314135
55-59	19.964736996767556	27.458125183661476	29.938289744343226	22.63884807522774
60-64	19.71201880693506	27.74022920952101	29.215398178078168	23.332353805465765
65-69	19.541580957978255	28.498383779018514	27.757860711137234	24.202174551866
70-74	20.946223920070526	28.351454598883336	27.511019688510135	23.191301792536
75-79	20.58667920757157	28.305214273117397	27.6056669214038	23.502439597907237
80-84	20.787244057425276	27.888915038832668	27.588844433984466	23.73499646975759
85-89	21.03001765744556	27.027663331371393	27.904649793996466	24.03766921718658
90-94	20.809282601013077	27.005536576746376	27.73000353398516	24.45517728825539
95-99	20.422493656694403	26.234731810939987	29.137900513365196	24.204874019000414
100-104	20.926385442514476	26.627673401866954	28.400094529126786	24.045846626491787
105-109	19.590314368598662	27.54129418033272	28.707595761056183	24.16079569001243
110-114	19.809026748116956	29.19755649131131	27.803807603345	23.189609157226737
115-119	19.980970504281636	29.17459562321599	27.8127973358706	23.03163653663178
120-124	20.04895814675503	29.846558003462892	27.380739148605887	22.72374470117619
125-129	19.957601453664445	27.843731072077528	30.496668685645062	21.70199878861296
130-134	18.447015834348356	33.15468940316687	30.74299634591961	17.655298416565167
135-139	21.07186358099878	25.889159561510354	30.194884287454325	22.84409257003654
140-144	21.065773447015836	28.605359317904995	28.599269183922043	21.729598051157126
145-149	20.444579780755177	29.17174177831912	29.04384896467722	21.33982947624848
150-154	20.164433617539586	29.116930572472594	29.20219244823386	21.51644336175396
155-159	19.859926918392205	28.81851400730816	29.488428745432397	21.833130328867234
160-164	20.219244823386116	28.471376370280144	29.33617539585871	21.97320341047503
165-169	20.66382460414129	28.611449451887943	28.885505481120582	21.839220462850186
170-174	20.23635477582846	28.386939571150098	29.367690058479536	22.00901559454191
175-179	19.62075483202244	28.425096030729836	29.687214194256452	22.26693494299128
180-184	19.962185898999756	27.67748231275921	29.409612100512323	22.950719687728714
185-189	20.362570957700054	27.675028993468842	28.962949398767012	22.99945065006409
190-194	19.980429331539355	27.42339918047826	29.625099382300778	22.971072105681607
195-199	20.308766770814188	27.764504074006002	29.47987502297372	22.44685413220609
200-204	20.67819558126654	27.047818327281682	28.79561819188873	23.47836789956305
205-209	20.742439266236985	26.654685176003966	29.58601883986118	23.01685671789787
210-214	21.12667250218832	26.978867075153186	28.61072902338377	23.28373139927473
215-219	20.985701632291534	27.192205491585476	28.603062128305705	23.219030747817285
220-224	21.087124132613724	27.08172706245181	29.009252120277562	22.8218966846569
225-229	20.90670170827858	26.373193166885677	29.54664914586071	23.17345597897503
230-234	20.78415411748745	27.553927553927554	29.446479446479444	22.21543888210555
235-239	19.66415014464122	27.53122133634375	29.478586043886263	23.326042475128766
240-244	20.911004209260373	27.71346963319302	28.998797354179196	22.376728803367406
245-249	21.110923233006424	27.942171119377747	28.973621914102132	21.973283733513696
250-252	18.408499566348656	28.751084128360798	31.04943625325238	21.79098005203816
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.3333333333333333
20	1.0
21	2.6666666666666665
22	4.333333333333333
23	4.666666666666666
24	7.666666666666663
25	11.000000000000002
26	13.33333333333334
27	17.66666666666667
28	19.0
29	24.16666666666665
30	25.83333333333331
31	33.333333333333286
32	44.00000000000003
33	46.333333333333385
34	55.33333333333345
35	71.66666666666673
36	69.50000000000011
37	68.50000000000013
38	78.49999999999999
39	78.33333333333336
40	75.33333333333343
41	71.83333333333346
42	71.66666666666679
43	63.1666666666668
44	63.50000000000012
45	60.83333333333345
46	65.50000000000014
47	54.00000000000007
48	53.83333333333339
49	57.83333333333342
50	60.16666666666677
51	54.66666666666673
52	51.33333333333338
53	43.49999999999999
54	39.16666666666665
55	31.333333333333297
56	25.999999999999982
57	25.33333333333332
58	25.333333333333318
59	19.500000000000007
60	16.33333333333334
61	17.500000000000007
62	12.166666666666666
63	7.499999999999998
64	6.499999999999998
65	2.6666666666666665
66	2.6666666666666665
67	4.5
68	5.0
69	3.1666666666666665
70	1.3333333333333333
71	1.6666666666666665
72	1.0
73	0.3333333333333333
74	0.3333333333333333
75	0.3333333333333333
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02938583602703497
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.005946363798537194
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	3.0
80-84	2.0
85-89	0.0
90-94	5.0
95-99	7.0
100-104	6.0
105-109	5.0
110-114	6.0
115-119	15.0
120-124	13.0
125-129	57.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	3.0
175-179	1.0
180-184	1.0
185-189	7.0
190-194	4.0
195-199	9.0
200-204	22.0
205-209	26.0
210-214	33.0
215-219	41.0
220-224	66.0
225-229	81.0
230-234	107.0
235-239	140.0
240-244	215.0
245-249	530.0
250-253	1998.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.94210990302675
#Duplication Level	Percentage of total
1	97.97237731413459
2	1.821921833676168
3	0.08815750808110491
4	0.11754334410813988
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGCGGCAAATTCGCCGTATGTCTCGTTTACGTCTGTTGTGTATTCGAT	4	0.11754334410813988	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.005877167205406994	0.0	0.0
25-29	0.0	0.0	0.0	0.041140170437848955	0.0	0.0
30-34	0.0	0.0	0.0	0.28210402585953565	0.07052600646488393	0.0
35-39	0.0	0.0	0.0	1.3752571260652364	0.1057890096973259	0.0
40-44	0.0	0.0	0.0	3.7790185130766973	0.1175433441081399	0.0
45-49	0.0	0.0	0.0	5.930061710255657	0.1175433441081399	0.0
50-54	0.0	0.0	0.0	8.551278283867175	0.14692918013517484	0.0
55-59	0.0	0.0	0.0	10.778724654716427	0.2527181898325007	0.0
60-64	0.0	0.0	0.0	12.829856009403468	0.39377020276226854	0.0
65-69	0.0	0.0	0.0	15.045548045841903	0.47017337643255946	0.0
70-74	0.0	0.0	0.0	17.32001175433441	0.5054363796650014	0.0
75-79	0.0	0.0	0.0	19.47693211871878	0.5289450484866295	0.0
80-84	0.0	0.0	0.0	21.310608286805763	0.5348222156920365	0.0
85-89	0.0	0.0	0.0	22.926829268292682	0.5583308845136644	0.0
90-94	0.0	0.0	0.0	24.88980311489862	0.5700852189244785	0.0
95-99	0.0	0.0	0.0	26.717602115780192	0.7346459006758742	0.0
100-104	0.0	0.0	0.0	28.798119306494264	0.987364090508375	0.0
105-109	0.0	0.0	0.0	30.467234792829856	1.3811342932706439	0.0
110-114	0.0	0.0	0.0	32.16573611519247	1.6103438142815165	0.0
115-119	0.0	0.0	0.0	33.14134587129004	1.8571848369086101	0.0
120-124	0.0	0.0	0.0	33.17660887452248	1.8806935057302383	0.0
125-129	0.0	0.0	0.0	33.17660887452248	1.8806935057302383	0.0
130-134	0.0	0.0	0.0	33.17660887452248	1.8806935057302383	0.0
135-139	0.0	0.0	0.0	33.17660887452248	1.8806935057302383	0.0
140-144	0.0	0.0	0.0	33.17660887452248	1.8806935057302383	0.0
145-149	0.0	0.0	0.0	33.188363208933296	1.8806935057302383	0.0
150-154	0.0	0.0	0.0	33.23538054657655	1.8806935057302383	0.0
155-159	0.0	0.0	0.0	33.28827505142521	1.8806935057302383	0.0
160-164	0.0	0.0	0.0	33.34116955627387	1.8806935057302383	0.0
165-169	0.0	0.0	0.0	33.3646782250955	1.8806935057302383	0.0
170-174	0.0	0.0	0.0	33.55862474287393	1.8806935057302383	0.0
175-179	0.0	0.0	0.0	33.664413752571264	1.8806935057302383	0.0
180-184	0.0	0.0	0.0	33.72906259183074	1.8806935057302383	0.0
185-189	0.0	0.0	0.0	33.81134293270644	1.8806935057302383	0.0
190-194	0.0	0.0	0.0	33.85248310314429	1.904202174551866	0.0
195-199	0.0	0.0	0.0	33.85248310314429	1.9100793417572732	0.0
200-204	0.0	0.0	0.0	33.88186893917132	1.91595650896268	0.0
205-209	0.0	0.0	0.0	33.946517778430795	1.939465177784308	0.0
210-214	0.0	0.0	0.0	34.16397296503085	1.9688510138113429	0.0
215-219	0.0	0.0	0.0	34.287393476344405	2.009991184249192	0.0
220-224	0.0	0.0	0.0	34.32853364678225	2.1099030267411107	0.0
225-229	0.0	0.0	0.0	34.42844548927417	2.1451660299735527	0.0
230-234	0.0	0.0	0.0	34.51660299735528	2.2039377020276225	0.0
235-239	0.0	0.0	0.0	34.53423449897149	2.2333235380546577	0.0
240-241	0.0	0.0	0.0	34.58712900382016	2.2333235380546577	0.0
>>END_MODULE
