##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665166
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	223959
Total Bases	55.4 Mbp
Sequences flagged as poor quality	0
Sequence length	76-252
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.502824177639656	27.0	18.0	32.0	18.0	33.0
2	26.38971865386075	28.0	25.0	31.0	18.0	33.0
3	25.49652838242714	27.0	18.0	30.0	18.0	31.0
4	30.34683580476784	31.0	29.0	33.0	27.0	33.0
5	31.946159788175514	33.0	32.0	33.0	30.0	33.0
6	36.01701650748574	37.0	36.0	38.0	31.0	38.0
7	36.149246067360544	38.0	36.0	38.0	33.0	38.0
8	36.708719006603886	38.0	37.0	38.0	34.0	38.0
9	36.878058037408636	38.0	38.0	38.0	35.0	38.0
10-14	36.93479431503088	38.0	38.0	38.0	35.0	38.0
15-19	36.914623658794696	38.0	38.0	38.0	35.0	38.0
20-24	36.91192762961078	38.0	38.0	38.0	35.0	38.0
25-29	36.845867323929824	38.0	38.0	38.0	35.0	38.0
30-34	36.829730441732636	38.0	38.0	38.0	35.0	38.0
35-39	36.768160243616016	38.0	38.0	38.0	35.0	38.0
40-44	36.7914689742319	38.0	38.0	38.0	35.0	38.0
45-49	36.754180899182444	38.0	38.0	38.0	35.0	38.0
50-54	36.66021995097317	38.0	38.0	38.0	34.0	38.0
55-59	36.57103219785764	38.0	38.0	38.0	34.0	38.0
60-64	36.53761090199545	38.0	38.0	38.0	34.0	38.0
65-69	36.47729182573595	38.0	38.0	38.0	34.0	38.0
70-74	36.39385423224787	38.0	37.6	38.0	34.0	38.0
75-79	36.29519261171631	38.0	37.0	38.0	33.4	38.0
80-84	36.13725808044875	38.0	37.0	38.0	33.0	38.0
85-89	36.00567606699979	38.0	37.0	38.0	31.8	38.0
90-94	35.9105215365946	38.0	37.0	38.0	31.0	38.0
95-99	35.80968146221212	38.0	37.0	38.0	31.0	38.0
100-104	35.68433068374942	38.0	36.6	38.0	30.2	38.0
105-109	35.48174746432362	38.0	36.0	38.0	29.0	38.0
110-114	35.349606184143816	38.0	35.8	38.0	29.0	38.0
115-119	35.18494226922353	38.0	35.0	38.0	28.8	38.0
120-124	35.351974335083725	38.0	35.0	38.0	29.8	38.0
125-129	33.925992986121	34.4	33.4	35.6	31.4	37.0
130-134	35.46804065499718	36.2	36.0	37.2	33.2	38.0
135-139	36.645959147822495	38.0	38.0	38.0	34.0	38.0
140-144	36.59238794331962	38.0	38.0	38.0	34.0	38.0
145-149	36.59054520358868	38.0	38.0	38.0	34.0	38.0
150-154	36.57941437444543	38.0	38.0	38.0	34.0	38.0
155-159	36.593343371604234	38.0	38.0	38.0	34.0	38.0
160-164	36.56672313194052	38.0	38.0	38.0	34.0	38.0
165-169	36.55289997160712	38.0	38.0	38.0	34.0	38.0
170-174	36.526598477591406	38.0	38.0	38.0	34.0	38.0
175-179	36.49905883546741	38.0	38.0	38.0	34.0	38.0
180-184	36.40194030703543	38.0	38.0	38.0	34.0	38.0
185-189	36.33008237664493	38.0	38.0	38.0	33.8	38.0
190-194	36.206022147193345	38.0	37.6	38.0	33.0	38.0
195-199	36.043157640405525	38.0	37.0	38.0	32.2	38.0
200-204	35.82455073933398	38.0	37.0	38.0	30.6	38.0
205-209	35.623467238869395	38.0	37.0	38.0	29.0	38.0
210-214	35.36082040548381	38.0	36.8	38.0	28.0	38.0
215-219	35.09037067301166	38.0	36.0	38.0	26.2	38.0
220-224	34.83890422617651	38.0	35.8	38.0	25.0	38.0
225-229	34.560340498610884	38.0	35.0	38.0	23.6	38.0
230-234	34.3819648057916	38.0	34.6	38.0	23.0	38.0
235-239	34.234157613940354	38.0	34.0	38.0	23.0	38.0
240-244	34.24751074339242	38.0	34.0	38.0	23.0	38.0
245-249	34.606110079968175	38.0	34.2	38.0	24.6	38.0
250-252	35.89530041232535	37.666666666666664	35.333333333333336	38.0	31.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	7.0
18	4.0
19	6.0
20	9.0
21	36.0
22	100.0
23	235.0
24	463.0
25	759.0
26	1283.0
27	1820.0
28	2559.0
29	3291.0
30	4194.0
31	5397.0
32	7124.0
33	9779.0
34	14586.0
35	24242.0
36	47945.0
37	100119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.46296999620205	26.44891758450437	17.77976363352025	18.30834878577333
2	11.318143052969516	23.819091887354382	45.734710371094714	19.128054688581393
3	12.463888479587782	34.58222263896516	36.790662576632336	16.16322630481472
4	7.65140047955206	22.773364767658364	52.58953647765886	16.985698275130716
5	6.920016610183113	43.73032563996089	42.25148353046763	7.09817421938837
6	16.323076991770815	53.75358882652629	23.084135935595356	6.839198246107546
7	16.539187976370677	38.03062167628896	34.09641943391424	11.333770913426118
8	16.260119039645648	44.04020378730035	26.000294696797184	13.699382476256815
9	18.698511781174233	21.596363620126898	30.864131381190308	28.840993217508558
10-14	15.890140606093079	30.46093258140999	37.266731857170285	16.382194955326646
15-19	15.791729736246365	35.829415205461714	32.999700838099834	15.37915422019209
20-24	15.982639602068243	34.39680654408416	33.66917011225319	15.951383741594405
25-29	16.035045825515652	33.58966092779813	34.3141711265763	16.061122120109914
30-34	15.991855652150615	33.78877383806858	34.3212820203698	15.898088489411007
35-39	15.867457882916069	33.532566228640064	34.20197446854112	16.398001419902748
40-44	15.649292950941915	32.224112449153644	34.93469786880634	17.1918967310981
45-49	15.106582484215792	32.04118629385867	35.23312406790559	17.61910715401995
50-54	14.626620955592449	32.28399686726136	34.71695956463149	18.3724226125147
55-59	14.487959742271956	31.145264492737446	34.27443661059936	20.092339154391244
60-64	15.082516208540964	31.25194234581793	32.5707728303774	21.09476861526371
65-69	15.624143236941258	31.14549723290649	31.115848207707497	22.11451132244476
70-74	16.027453537076564	30.714560020005177	30.380902197890524	22.87708424502773
75-79	16.15465269144482	30.305636529915265	30.13612623426359	23.40358454437633
80-84	16.167595151043184	29.637792856594263	30.395196767838424	23.799415224524132
85-89	16.309139362416587	28.573278510495403	30.879106354400353	24.238475772687654
90-94	16.798177338009666	27.58535690076013	30.91380491407821	24.702660847152003
95-99	16.8566421761708	26.784671200355838	31.511997842121154	24.84668878135221
100-104	16.849405247329038	26.95301020686156	31.610811930960992	24.586772614848407
105-109	16.545061502318063	29.231064700259946	30.707388317686046	23.516485479735945
110-114	16.020680376156278	31.543202045523987	29.81831858928887	22.61779898903086
115-119	15.769740098899312	32.17989523892923	29.80293409631513	22.247430565856327
120-124	15.773667204447642	32.61428590581831	29.97081681630683	21.641230073427213
125-129	15.73022838363291	32.225945462737286	34.079922179197446	17.963903974432355
130-134	13.884185209720346	38.840417836112366	34.72310245016615	12.552294504001141
135-139	16.577600980947533	29.997597825853127	36.312178664391176	17.112622528808163
140-144	15.568114084424984	34.45989611576952	33.65140969735539	16.32058010245011
145-149	15.890687714782024	33.85018455344162	34.208266631769725	16.05086110000663
150-154	15.792832570554777	33.412569084791215	34.563011245381645	16.231587099272357
155-159	15.690932004825939	33.16550709100981	34.5167518496143	16.626809054549952
160-164	15.612876739833492	32.66001807060444	34.75310678149627	16.9739984080658
165-169	15.659957262505737	32.619786886186326	34.08817117944011	17.63208467186783
170-174	15.658203499638779	32.57324377183689	33.87059475215523	17.897957976369103
175-179	16.039189498075036	31.546559938329427	33.76652130458361	18.64772925901193
180-184	16.391491146845034	30.888291497511183	33.57233968362958	19.147877672014204
185-189	16.115668025522705	30.516882299517928	33.81606044115155	19.55138923380781
190-194	16.544721972374347	30.207968089618813	33.39326693109191	19.85404300691493
195-199	16.49527821988082	29.92287968477206	33.30187274250822	20.2799693528389
200-204	17.60515137830075	29.097171033552733	32.6133350811053	20.68434250704122
205-209	18.189475766034242	28.059439852209444	32.48254875784913	21.26853562390719
210-214	18.38225159429899	28.4033407534262	31.895258677770084	21.319148974504728
215-219	18.383693670226624	28.578767618882235	31.421666521953057	21.61587218893809
220-224	18.85941335218731	28.198600893984644	31.815904034228716	21.12608171959933
225-229	18.573172688392955	29.301718271364052	31.0890561402797	21.036052899963302
230-234	18.92312427813406	29.497783689195	30.971778630603257	20.60731340206768
235-239	19.22016178466339	29.36390669064196	30.91074060150011	20.505190923194544
240-244	18.85821449263577	29.34072635976459	30.85433162907472	20.94672751852492
245-249	18.558073610679436	30.412514300451665	30.773249393264074	20.25616269560483
250-252	16.428079906581033	31.49084577529402	32.35335515889565	19.72771915922929
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.5
16	4.833333333333333
17	16.000000000000007
18	43.33333333333336
19	95.16666666666644
20	195.00000000000102
21	358.4999999999974
22	602.3333333333312
23	1016.5000000000439
24	1511.3333333332728
25	2079.499999999824
26	2756.833333333463
27	3437.833333333771
28	4148.833333334044
29	4856.50000000007
30	5486.8333333328355
31	5987.999999999049
32	6517.833333331894
33	6595.833333331822
34	6688.499999998402
35	6926.166666664852
36	6847.166666664925
37	6875.666666664896
38	6948.333333331493
39	6447.499999998617
40	5736.666666665934
41	4841.66666666675
42	4000.1666666673545
43	3139.500000000293
44	2395.8333333332903
45	1792.9999999998804
46	1283.1666666666613
47	867.6666666666914
48	545.3333333333235
49	375.9999999999983
50	263.66666666666845
51	201.666666666667
52	163.83333333333286
53	134.16666666666623
54	116.66666666666646
55	95.66666666666667
56	72.33333333333346
57	57.33333333333339
58	44.33333333333332
59	37.833333333333286
60	25.833333333333318
61	25.833333333333318
62	17.333333333333343
63	15.33333333333334
64	17.833333333333343
65	10.33333333333333
66	7.999999999999997
67	4.666666666666667
68	1.3333333333333333
69	2.6666666666666665
70	3.0
71	2.1666666666666665
72	1.8333333333333333
73	0.0
74	1.0
75	1.3333333333333333
76	0.0
77	0.0
78	0.0
79	0.3333333333333333
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0687625860090463
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	4.465102987600409E-4
25-29	3.572082390080327E-4
30-34	0.0
35-39	0.0
40-44	0.0
45-49	4.465102987600409E-4
50-54	7.144164780160654E-4
55-59	8.930205975200818E-4
60-64	0.0013395308962801226
65-69	0.002500457673056229
70-74	0.007590675078920695
75-79	0.008037249974548709
80-84	0.0016967845931958937
85-89	3.572283367805868E-4
90-94	7.144841112130243E-4
95-99	1.786320358693128E-4
100-104	0.0018757335457973744
105-109	3.5731066554471115E-4
110-114	5.360359072586409E-4
115-119	5.361336187947001E-4
120-124	0.00196636268487161
125-129	0.020776559742012445
130-134	0.015505543455854014
135-139	0.0066324289926774395
140-144	0.005825782223297752
145-149	0.005736154804477786
150-154	0.00645317415503751
155-159	0.008245722531436817
160-164	0.009500506394916333
165-169	0.007170206358538999
170-174	0.005646572933555969
175-179	0.008694077809307503
180-184	0.0036750469688929684
185-189	0.0041239146708264415
190-194	0.0038559281757155435
195-199	0.0026017112082615994
200-204	0.0019751827268474917
205-209	0.0
210-214	6.305988166362778E-4
215-219	6.33118043050218E-4
220-224	8.199954991358158E-4
225-229	6.4547718745303E-4
230-234	2.8239174512450653E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	5.0
80-84	5.0
85-89	7.0
90-94	15.0
95-99	9.0
100-104	15.0
105-109	24.0
110-114	37.0
115-119	51.0
120-124	108.0
125-129	537.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	1.0
170-174	3.0
175-179	6.0
180-184	27.0
185-189	47.0
190-194	80.0
195-199	139.0
200-204	225.0
205-209	381.0
210-214	637.0
215-219	1301.0
220-224	2101.0
225-229	3594.0
230-234	5752.0
235-239	7857.0
240-244	11673.0
245-249	27968.0
250-253	161354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.24936801051719
#Duplication Level	Percentage of total
1	30.681481941227627
2	14.346176327643617
3	9.844756914017736
4	7.58307514883803
5	5.739643768146141
6	4.484111473525551
7	3.6844543517755652
8	3.1741251456145556
9	2.7439030170596133
>10	15.736799786660285
>50	1.0305539924968874
>100	0.9509181329943843
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTTATAGTAATATCTTTAGTAGTTGAATCAATAGTAGCACGAATAACA	257	0.1147531467813305	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	4.465102987600409E-4	0.0	0.0
7	0.0	0.0	0.0	4.465102987600409E-4	0.0	0.0
8	0.0	0.0	0.0	4.465102987600409E-4	0.0	0.0
9	0.0	0.0	0.0	4.465102987600409E-4	0.0	0.0
10-14	0.0	0.0	0.0	4.465102987600409E-4	0.0	0.0
15-19	0.0	0.0	0.0	4.465102987600409E-4	0.0	0.0
20-24	0.0	0.0	0.0	0.0012502288365281145	0.0	0.0
25-29	0.0	0.0	0.0	0.034470595064275154	0.0	0.0
30-34	0.0	0.0	0.0	0.6896798074647592	8.037185377680736E-4	0.0
35-39	0.0	0.0	2.6790617925602457E-4	2.6888850191329667	8.930205975200818E-4	0.0
40-44	0.0	0.0	4.465102987600409E-4	6.7064060832563115	8.930205975200818E-4	0.0
45-49	0.0	0.0	4.465102987600409E-4	11.060774516764228	0.001428832956032131	0.0
50-54	0.0	0.0	4.465102987600409E-4	15.341200844797488	0.005358123585120491	0.0
55-59	0.0	0.0	4.465102987600409E-4	19.315410409941105	0.0076799771386727046	0.0
60-64	0.0	0.0	4.465102987600409E-4	23.265687023071187	0.010359038931232948	0.0
65-69	0.0	0.0	4.465102987600409E-4	26.94618211369045	0.011877173947017088	0.0
70-74	0.0	0.0	4.465102987600409E-4	30.517728691412266	0.012591590425033152	0.0
75-79	0.0	0.0	4.465102987600409E-4	33.89986560040008	0.013841819261561267	0.0
80-84	0.0	0.0	4.465102987600409E-4	37.12527739452311	0.015002746038337372	0.0
85-89	0.0	0.0	4.465102987600409E-4	40.13431029786702	0.01625297487486549	0.0
90-94	0.0	0.0	4.465102987600409E-4	43.06573971128644	0.01821762018940967	0.0
95-99	0.0	0.0	4.465102987600409E-4	45.823208712308954	0.08921275769225616	0.0
100-104	0.0	0.0	4.465102987600409E-4	48.43493675181618	0.34568827330002366	0.0
105-109	0.0	0.0	4.465102987600409E-4	50.868596484177914	0.9037368446903228	0.0
110-114	0.0	0.0	4.465102987600409E-4	53.202773721975895	1.4784849012542474	0.0
115-119	0.0	0.0	4.465102987600409E-4	54.610888600145564	1.9033841015543023	0.0
120-124	0.0	0.0	4.465102987600409E-4	54.67170330283668	1.9409802687098978	0.0
125-129	0.0	0.0	4.465102987600409E-4	54.672685625493955	1.9409802687098978	0.0
130-134	0.0	0.0	4.465102987600409E-4	54.67438236462924	1.9409802687098978	0.0
135-139	0.0	0.0	4.465102987600409E-4	54.67768654084006	1.9409802687098978	0.0
140-144	0.0	0.0	4.465102987600409E-4	54.68340187266419	1.9409802687098978	0.0
145-149	0.0	0.0	4.465102987600409E-4	54.692778588938154	1.9409802687098978	0.0
150-154	0.0	0.0	4.465102987600409E-4	54.70670971025946	1.9409802687098978	0.0
155-159	0.0	0.0	4.465102987600409E-4	54.72653476752441	1.9409802687098978	0.0
160-164	0.0	0.0	4.465102987600409E-4	54.747877959805145	1.9409802687098978	0.0
165-169	0.0	0.0	4.465102987600409E-4	54.77011417268339	1.9409802687098978	0.0
170-174	0.0	0.0	4.465102987600409E-4	54.80387035126965	1.9409802687098978	0.0
175-179	0.0	0.0	4.465102987600409E-4	54.84655673583111	1.941337476948906	0.0
180-184	0.0	0.0	4.465102987600409E-4	54.899870065503066	1.941426779008658	0.0
185-189	0.0	0.0	4.465102987600409E-4	54.944967605677824	1.941426779008658	0.0
190-194	0.0	0.0	4.465102987600409E-4	54.9924763014659	1.9416053831281619	0.0
195-199	0.0	0.0	4.465102987600409E-4	55.03873476841743	1.943570028442706	0.0
200-204	0.0	0.0	4.465102987600409E-4	55.08124254885939	1.9508034952826185	0.0
205-209	0.0	0.0	4.465102987600409E-4	55.13160891055952	2.0031345022972955	0.0
210-214	0.0	0.0	4.465102987600409E-4	55.172419951866196	2.1123509213740013	0.0
215-219	0.0	0.0	4.465102987600409E-4	55.209926816962025	2.25193004076639	0.0
220-224	0.0	0.0	4.465102987600409E-4	55.241361141994744	2.480007501373019	0.0
225-229	0.0	0.0	4.465102987600409E-4	55.26931268669712	2.7345183716662427	0.0
230-234	0.0	0.0	4.465102987600409E-4	55.30128282408833	2.952504699520895	0.0
235-239	0.0	0.0	4.465102987600409E-4	55.33584272121236	3.0905656838974993	0.0
240-241	0.0	0.0	4.465102987600409E-4	55.39339789872253	3.0945396255564637	0.0
>>END_MODULE
