##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665168
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4958810
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.956134032156907	32.0	18.0	33.0	18.0	33.0
2	28.91290813723454	31.0	27.0	33.0	18.0	33.0
3	30.623257596076478	33.0	29.0	33.0	27.0	33.0
4	31.705870763348464	33.0	31.0	33.0	29.0	33.0
5	31.838519523837373	33.0	32.0	33.0	30.0	34.0
6	35.5954132140574	37.0	36.0	38.0	31.0	38.0
7	36.202665558874	38.0	37.0	38.0	33.0	38.0
8	36.667865475789554	38.0	37.0	38.0	34.0	38.0
9	36.81657897761762	38.0	38.0	38.0	35.0	38.0
10-14	36.891408785575564	38.0	38.0	38.0	35.0	38.0
15-19	36.860838830283875	38.0	38.0	38.0	35.0	38.0
20-24	36.85456232442864	38.0	38.0	38.0	35.0	38.0
25-29	36.812565434045666	38.0	38.0	38.0	35.0	38.0
30-34	36.78090949239838	38.0	38.0	38.0	35.0	38.0
35-39	36.727275979519284	38.0	38.0	38.0	34.6	38.0
40-44	36.74791145456269	38.0	38.0	38.0	35.0	38.0
45-49	36.708613921485195	38.0	38.0	38.0	34.2	38.0
50-54	36.61780100467653	38.0	38.0	38.0	34.0	38.0
55-59	36.52089444846647	38.0	38.0	38.0	34.0	38.0
60-64	36.503118530453875	38.0	38.0	38.0	34.0	38.0
65-69	36.443773606974254	38.0	38.0	38.0	34.0	38.0
70-74	36.35547685835916	38.0	37.6	38.0	33.4	38.0
75-79	36.22918236349604	38.0	37.0	38.0	33.0	38.0
80-84	36.055789839773624	38.0	37.0	38.0	32.2	38.0
85-89	35.942281478697886	38.0	37.0	38.0	31.0	38.0
90-94	35.842344670158525	38.0	37.0	38.0	31.0	38.0
95-99	35.73457425030087	38.0	37.0	38.0	30.8	38.0
100-104	35.61119360797805	38.0	36.6	38.0	29.0	38.0
105-109	35.425555198869986	38.0	36.0	38.0	29.0	38.0
110-114	35.29735601969943	38.0	35.8	38.0	28.2	38.0
115-119	35.132720787689635	38.0	35.0	38.0	28.0	38.0
120-124	35.29876850821452	38.0	35.0	38.0	29.2	38.0
125-129	33.829226933247966	34.4	33.4	35.6	31.2	36.2
130-134	35.300972244342155	36.2	36.0	37.2	33.0	38.0
135-139	36.488483324210144	38.0	38.0	38.0	34.0	38.0
140-144	36.42893854762327	38.0	38.0	38.0	34.0	38.0
145-149	36.42318950351137	38.0	38.0	38.0	34.0	38.0
150-154	36.41948887754952	38.0	38.0	38.0	34.0	38.0
155-159	36.42494677566276	38.0	38.0	38.0	34.0	38.0
160-164	36.40502589273834	38.0	38.0	38.0	34.0	38.0
165-169	36.389643558705316	38.0	38.0	38.0	34.0	38.0
170-174	36.366898749507584	38.0	38.0	38.0	34.0	38.0
175-179	36.33982766550039	38.0	38.0	38.0	33.8	38.0
180-184	36.24506610232826	38.0	37.8	38.0	33.0	38.0
185-189	36.18949470535342	38.0	37.0	38.0	33.0	38.0
190-194	36.12741521530483	38.0	37.0	38.0	33.0	38.0
195-199	36.040192387524726	38.0	37.0	38.0	32.0	38.0
200-204	35.9043580532885	38.0	37.0	38.0	31.0	38.0
205-209	35.78554395525646	38.0	37.0	38.0	30.8	38.0
210-214	35.634642891872026	38.0	37.0	38.0	29.0	38.0
215-219	35.44531940462369	38.0	36.4	38.0	29.0	38.0
220-224	35.302767576746376	38.0	36.0	38.0	28.0	38.0
225-229	35.10451486873701	38.0	36.0	38.0	27.0	38.0
230-234	34.96225101347612	38.0	35.2	38.0	26.0	38.0
235-239	34.82001578838266	38.0	35.0	38.0	25.2	38.0
240-244	34.765314964312175	38.0	35.0	38.0	25.0	38.0
245-249	34.97333388142685	38.0	35.0	38.0	27.0	38.0
250-252	35.9775582170945	37.666666666666664	35.333333333333336	38.0	31.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	3.0
6	1.0
7	0.0
8	2.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	13.0
17	33.0
18	64.0
19	113.0
20	237.0
21	752.0
22	2221.0
23	5427.0
24	10846.0
25	19283.0
26	29154.0
27	42925.0
28	57798.0
29	75902.0
30	97190.0
31	123435.0
32	158632.0
33	208604.0
34	291599.0
35	460389.0
36	956510.0
37	2417674.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.38819960065341	30.753865075737625	14.540473949142333	22.31746137446664
2	12.949009765117298	21.87601650650272	43.786638347833225	21.38833538054676
3	13.264832627212211	31.796887178522304	34.936610429055044	20.00166976521044
4	11.21892884943474	20.139953778953696	45.67809017540605	22.96302719620552
5	9.978284972628337	39.45397434609898	40.30662393033122	10.261116750941463
6	22.345719792804793	47.31937270332633	20.015862702322053	10.319044801546825
7	19.91211984998787	34.71895110086132	31.47211653466898	13.896812514481832
8	18.777457516409726	42.254905776542174	23.39533156933022	15.57230513771788
9	21.182745825602968	18.147253367750263	27.191074453496817	33.47892635314996
10-14	19.352377005188313	27.78305616203086	33.52889145366783	19.335675379112995
15-19	18.71389927861173	33.195342953752636	30.101896833987933	17.988860933647693
20-24	18.680098688854073	31.384630983995894	31.24209179973577	18.69317852741426
25-29	18.737239956674486	31.250057222186033	31.136407406638096	18.87629541450138
30-34	18.687250418347347	31.10949871319622	31.36577049518855	18.837480373267876
35-39	18.57415924480728	30.620280584943394	31.50302276894613	19.302537401303194
40-44	18.45165480203209	29.887658676034956	31.965629739429225	19.69505678250373
45-49	17.74779782922615	29.996340621987784	32.38921407506569	19.866647473720377
50-54	17.230840899099366	29.846686859968457	32.15401896449501	20.76845327643716
55-59	17.184314879808674	29.16692194179356	31.719517255946528	21.92924592245124
60-64	17.523330796514266	29.18654501497307	30.6175118277895	22.672612360723168
65-69	17.741097514872013	29.052614706646207	29.48404611308597	23.722241665395813
70-74	18.40903519878287	29.55300986063835	28.224262634332387	23.8136923062464
75-79	18.92042709775728	29.6109887187874	27.6362642305951	23.83231995286022
80-84	19.332459962198733	29.197719339712062	27.517531285267268	23.95228941282194
85-89	19.648648889562555	28.450903657112253	27.64764392755255	24.25280352577264
90-94	19.887009984035757	27.49989623785696	27.73912797609288	24.873965802014407
95-99	19.804647677770518	26.82132842031538	28.315417882558208	25.0586060193559
100-104	19.877923622110707	27.061381149068897	28.29136636814103	24.76932886067937
105-109	19.389523914762545	28.86866633754491	27.57626432600499	24.165545421687558
110-114	18.984087178332903	30.473280109919344	26.89290633362824	23.649726378119514
115-119	18.894014948996755	30.974358435860143	26.76913626268619	23.36249035245691
120-124	18.883075982969117	31.297861646927238	26.79039305988709	23.02866931021655
125-129	19.188633088184005	29.303618988256247	30.883630826239855	20.624117097319893
130-134	17.17462223604843	35.70311745322301	31.5798374708704	15.542422839858158
135-139	19.72528995381318	27.382836892901047	32.693221640838516	20.198651512447256
140-144	18.35786125021235	31.708855890198766	30.891442456553403	19.041840403035483
145-149	18.49582824617403	31.26066982999638	31.63420881947701	18.60929310435258
150-154	18.364187402021074	30.898898050160216	31.736985751247975	18.99992879657074
155-159	18.353757306852593	30.575650594457194	31.77119417187912	19.299397926811093
160-164	18.218619702035927	30.449257992973372	31.841071140551453	19.491051164439252
165-169	18.26413564651978	30.327106783554374	31.458224655335492	19.950532914590354
170-174	18.237172844026357	30.42052554597002	31.36509026569486	19.97721134430876
175-179	18.545444286338494	29.61192075633926	31.334300149551186	20.50833480777106
180-184	18.863527151195488	29.25278158205663	31.19192924379817	20.69176202294971
185-189	18.633682391938645	28.755646255249882	31.8770193275629	20.733652025248574
190-194	18.91497204375896	28.591607918756385	31.408854005423187	21.08456603206147
195-199	18.931399284123064	28.494077712208266	31.530540105651095	21.04398289801758
200-204	19.5237381174209	27.613474994243592	30.919189858232563	21.943597030102943
205-209	19.959861577792587	26.923467836680494	31.013806756251743	22.102863829275176
210-214	20.36999768203364	27.25119652799349	30.23399964807638	22.144806141896492
215-219	20.376298896828086	27.633551349728826	29.82999945042038	22.160150303022714
220-224	21.000334633691757	27.355589271775653	30.364971243181365	21.27910485135122
225-229	20.72215960550506	27.914953690708433	29.901080462972	21.461806240814507
230-234	21.1886125693603	28.151748953704995	29.55594303518927	21.103695441745433
235-239	21.401287953007987	27.50503187738867	29.860097475083325	21.233582694520024
240-244	21.703970974624433	27.302508905219952	29.49613591370931	21.497384206446306
245-249	21.074766759226343	28.01148188360541	29.500259423311586	21.413491933856662
250-252	19.84415211576063	29.419699551449952	29.980755534541547	20.755392798247875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.5
5	1.5
6	1.5
7	1.0
8	0.5
9	0.5
10	1.0
11	1.0
12	0.5
13	0.3333333333333333
14	1.0
15	4.0
16	9.666666666666664
17	14.83333333333334
18	35.8333333333333
19	121.83333333333272
20	311.3333333333333
21	595.9999999999972
22	1019.6666666667112
23	1637.666666666578
24	2918.1666666668652
25	5115.8333333332
26	8753.333333331146
27	14467.666666674815
28	22837.83333332231
29	33888.99999995923
30	47974.166666727775
31	67230.00000017253
32	88630.83333319996
33	107795.66666625981
34	128173.33333263066
35	147644.49999968178
36	162347.83333344196
37	173217.16666709806
38	178071.8333339084
39	175783.1666671766
40	166287.1666669014
41	154302.9999998838
42	137152.16666604788
43	115737.4999994797
44	94407.83333312438
45	76306.16666672178
46	61371.66666681344
47	48477.666666717625
48	35752.166666621444
49	27014.99999998485
50	22510.833333336705
51	20793.666666678473
52	18836.833333342995
53	16306.166666673496
54	13858.833333336059
55	12022.500000000085
56	10103.166666664334
57	8996.83333333096
58	8967.33333333132
59	9185.333333331462
60	8461.499999998197
61	8460.666666664845
62	8896.49999999841
63	8492.499999998414
64	9339.833333332444
65	8551.833333333125
66	5661.333333333969
67	5725.500000000518
68	4968.666666666952
69	3055.8333333333603
70	2097.83333333315
71	1676.1666666665699
72	1324.666666666648
73	1010.3333333333771
74	746.5000000000142
75	533.4999999999899
76	344.3333333333315
77	229.3333333333354
78	156.33333333333334
79	97.99999999999973
80	56.33333333333344
81	26.833333333333307
82	6.166666666666665
83	4.166666666666667
84	1.0
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06483410334334246
2	7.461467569840345E-4
3	5.84817728446946E-4
4	6.04983857014082E-4
5	6.453161141483541E-4
6	1.6132902853708853E-4
7	2.218274142384967E-4
8	2.621596713727689E-4
9	2.0166128567136065E-4
10-14	2.2989386566535115E-4
15-19	2.056945113847879E-4
20-24	6.695154684289174E-4
25-29	0.0013914628711323887
30-34	2.4199354280563282E-4
35-39	2.4602676851906E-4
40-44	2.2989386566535115E-4
45-49	8.832764312405598E-4
50-54	0.0014116289996995248
55-59	0.002173908659537268
60-64	0.0021497093052567045
65-69	0.00403725893914064
70-74	0.010700147817722397
75-79	0.012011004306295937
80-84	0.0032185701981856784
85-89	6.332398316404856E-4
90-94	0.0012584404752210045
95-99	2.7024967722306275E-4
100-104	0.002868003468226979
105-109	5.083247250961732E-4
110-114	5.408116008124281E-4
115-119	0.0010056364509530707
120-124	0.0019406374371359813
125-129	0.023364563164030856
130-134	0.017699129536911672
135-139	0.008847517679682193
140-144	0.0068413706815127
145-149	0.006178113918852582
150-154	0.007954988928522356
155-159	0.010751320803445466
160-164	0.013130056422804357
165-169	0.010284623685128056
170-174	0.008200721229480951
175-179	0.01106678302005835
180-184	0.004847695559478113
185-189	0.00567091163405333
190-194	0.004655880665970291
195-199	0.00352220704442228
200-204	0.0026466487158878322
205-209	2.1319647650183286E-4
210-214	5.952942278670529E-4
215-219	5.067312657022321E-4
220-224	6.549210581783371E-4
225-229	3.932850839477472E-4
230-234	2.1700476576505586E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	59.0
80-84	79.0
85-89	109.0
90-94	121.0
95-99	181.0
100-104	336.0
105-109	1384.0
110-114	2897.0
115-119	4223.0
120-124	9356.0
125-129	55079.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	4.0
155-159	3.0
160-164	10.0
165-169	17.0
170-174	35.0
175-179	78.0
180-184	152.0
185-189	323.0
190-194	641.0
195-199	1160.0
200-204	2799.0
205-209	4555.0
210-214	11215.0
215-219	25364.0
220-224	36678.0
225-229	61916.0
230-234	105546.0
235-239	128269.0
240-244	194591.0
245-249	567993.0
250-253	3743637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.732961617636075
#Duplication Level	Percentage of total
1	14.546618322587024
2	5.366148193084558
3	3.2525597243684814
4	2.4193918187042445
5	2.1116044884487595
6	1.7639181418862135
7	1.5732541675394864
8	1.353410815238325
9	1.3227108255082942
>10	25.463805506997012
>50	14.875239333256483
>100	23.386239689798206
>500	1.640997689173317
>1k	0.9241012834096831
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.033225713427213E-5	0.0	0.0
5	0.0	0.0	0.0	6.04983857014082E-5	2.0166128567136066E-5	0.0
6	0.0	0.0	0.0	6.04983857014082E-5	4.033225713427213E-5	0.0
7	0.0	0.0	0.0	6.04983857014082E-5	4.033225713427213E-5	0.0
8	0.0	0.0	0.0	6.04983857014082E-5	6.04983857014082E-5	0.0
9	0.0	0.0	0.0	6.04983857014082E-5	6.04983857014082E-5	0.0
10-14	0.0	0.0	0.0	1.250299971162436E-4	2.7829257422647775E-4	0.0
15-19	1.6132902853708852E-5	0.0	0.0	2.2182741423849676E-4	5.565851484529555E-4	4.033225713427213E-6
20-24	3.2265805707417705E-5	0.0	8.066451426854426E-6	6.291832112946454E-4	6.654822427154901E-4	4.033225713427213E-5
25-29	4.033225713427213E-5	0.0	4.436548284769935E-5	0.023191047852206475	7.94545465545161E-4	4.033225713427213E-5
30-34	6.04983857014082E-5	0.0	6.04983857014082E-5	0.6417063771348368	0.0012906322282967083	4.436548284769935E-5
35-39	6.04983857014082E-5	0.0	6.04983857014082E-5	2.4273565633690337	0.0014640609339740785	6.04983857014082E-5
40-44	6.04983857014082E-5	0.0	6.04983857014082E-5	5.911458595913132	0.0015285925453889138	6.856483712826263E-5
45-49	6.04983857014082E-5	4.033225713427213E-6	6.04983857014082E-5	9.533299319796482	0.001589090931090322	8.066451426854427E-5
50-54	6.04983857014082E-5	2.0166128567136066E-5	6.04983857014082E-5	13.381210411368858	0.0016697554453588663	8.066451426854427E-5
55-59	6.856483712826263E-5	2.0166128567136066E-5	6.04983857014082E-5	17.090015548085127	0.0018310844738959546	1.0083064283568034E-4
60-64	1.209967714028164E-4	2.0166128567136066E-5	6.04983857014082E-5	20.919978785232747	0.0024965667166114446	1.4116289996995247E-4
65-69	1.209967714028164E-4	2.0166128567136066E-5	6.04983857014082E-5	24.61289301263811	0.002754693162270787	1.6132902853708853E-4
70-74	1.2906322282967082E-4	2.0166128567136066E-5	6.04983857014082E-5	28.368185108927342	0.0029482879965152934	1.6132902853708853E-4
75-79	1.8552838281765184E-4	2.0166128567136066E-5	6.04983857014082E-5	32.02715570872851	0.0030652515422046824	1.7342870567737018E-4
80-84	2.218274142384967E-4	2.0166128567136066E-5	6.04983857014082E-5	35.687308043663705	0.0032386802478820524	1.814951571042246E-4
85-89	2.218274142384967E-4	2.0166128567136066E-5	6.04983857014082E-5	39.20702749248308	0.0034887402421145395	2.097277370982151E-4
90-94	2.3796031709220558E-4	2.0166128567136066E-5	6.04983857014082E-5	42.63689070563301	0.0052189940731748145	2.218274142384967E-4
95-99	2.419935428056328E-4	2.0166128567136066E-5	6.04983857014082E-5	45.99234897082163	0.0727997241273612	2.218274142384967E-4
100-104	2.419935428056328E-4	2.0166128567136066E-5	6.04983857014082E-5	49.21473095359572	0.3168905443039762	2.218274142384967E-4
105-109	2.419935428056328E-4	2.0166128567136066E-5	6.04983857014082E-5	52.327364831481745	0.8288843492692803	2.3796031709220558E-4
110-114	2.419935428056328E-4	2.0166128567136066E-5	6.04983857014082E-5	55.28201725817282	1.3183606550765203	2.419935428056328E-4
115-119	2.419935428056328E-4	2.0166128567136066E-5	6.04983857014082E-5	57.045190277506094	1.6880662900978258	2.419935428056328E-4
120-124	2.581264456593417E-4	2.0166128567136066E-5	6.04983857014082E-5	57.096807500186536	1.7244016205500916	2.419935428056328E-4
125-129	2.6619289708619613E-4	2.0166128567136066E-5	6.04983857014082E-5	57.097436683397824	1.7244903515157872	2.500599942324872E-4
130-134	2.984587027936138E-4	2.0166128567136066E-5	6.04983857014082E-5	57.09846112272904	1.7245911821586226	2.621596713727689E-4
135-139	3.02491928507041E-4	2.0166128567136066E-5	6.04983857014082E-5	57.1018449991026	1.724687979575745	2.6619289708619613E-4
140-144	3.02491928507041E-4	2.0166128567136066E-5	6.04983857014082E-5	57.107556046712816	1.7247968766700077	2.9442547708018657E-4
145-149	3.02491928507041E-4	2.0166128567136066E-5	6.04983857014082E-5	57.11550553459399	1.7249461060214042	3.02491928507041E-4
150-154	3.065251542204682E-4	2.0166128567136066E-5	6.04983857014082E-5	57.126104851768865	1.7250469366642398	3.02491928507041E-4
155-159	3.428241856413131E-4	2.0166128567136066E-5	6.04983857014082E-5	57.14008401209162	1.7251155015013684	3.02491928507041E-4
160-164	3.428241856413131E-4	2.419935428056328E-5	6.04983857014082E-5	57.15769307555644	1.7251921327899233	3.02491928507041E-4
165-169	3.5089063706816756E-4	4.839870856112656E-5	6.04983857014082E-5	57.179206301511854	1.7253050631098994	3.2265805707417706E-4
170-174	3.831564427755853E-4	6.04983857014082E-5	6.04983857014082E-5	57.20668870152315	1.7253937940755946	3.3072450850103144E-4
175-179	3.831564427755853E-4	6.04983857014082E-5	6.04983857014082E-5	57.24312082939254	1.7254502592355827	3.629903142084492E-4
180-184	4.0332257134272136E-4	6.04983857014082E-5	6.04983857014082E-5	57.2896682873512	1.7255309237498515	3.629903142084492E-4
185-189	4.0332257134272136E-4	6.04983857014082E-5	6.04983857014082E-5	57.33555832951858	1.7256922527783884	3.831564427755853E-4
190-194	4.0332257134272136E-4	6.04983857014082E-5	6.04983857014082E-5	57.38181136200016	1.7258737479354926	3.9122289420243973E-4
195-199	4.0332257134272136E-4	6.04983857014082E-5	6.04983857014082E-5	57.428947670912976	1.7263819343753846	4.0332257134272136E-4
200-204	4.1138902276957574E-4	6.04983857014082E-5	6.04983857014082E-5	57.475664524351615	1.7294875181747233	4.0332257134272136E-4
205-209	4.3155515133671185E-4	6.04983857014082E-5	6.04983857014082E-5	57.52400273452704	1.7471853126052423	4.1138902276957574E-4
210-214	4.436548284769934E-4	6.04983857014082E-5	6.04983857014082E-5	57.56546429486106	1.7770836148188778	4.3962160276356623E-4
215-219	4.678541827575568E-4	8.066451426854427E-5	6.04983857014082E-5	57.60510283717262	1.8117290236972177	4.436548284769934E-4
220-224	4.839870856112656E-4	8.066451426854427E-5	6.04983857014082E-5	57.63821965350559	1.866996315648311	4.436548284769934E-4
225-229	5.041532141784017E-4	8.066451426854427E-5	6.04983857014082E-5	57.675268864909114	1.925833012355787	4.436548284769934E-4
230-234	5.041532141784017E-4	8.066451426854427E-5	6.04983857014082E-5	57.71874703809986	1.9700653987549432	4.436548284769934E-4
235-239	5.041532141784017E-4	8.066451426854427E-5	6.453161141483541E-5	57.76868643888351	1.9951520626924606	4.436548284769934E-4
240-241	5.041532141784017E-4	8.066451426854427E-5	8.066451426854427E-5	57.84185116993795	1.9966584724964256	4.436548284769935E-4
>>END_MODULE
