##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665169
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6934
Total Bases	1.6 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.22411306605134	28.0	18.0	32.0	18.0	33.0
2	27.488606864724545	29.0	25.0	31.0	18.0	33.0
3	29.014565907124314	31.0	27.0	33.0	18.0	33.0
4	30.135852321892127	31.0	29.0	33.0	25.0	33.0
5	30.378713585232187	33.0	31.0	33.0	25.0	33.0
6	33.50605710989328	36.0	31.0	38.0	26.0	38.0
7	34.200028843380444	37.0	34.0	38.0	26.0	38.0
8	34.7334871646957	38.0	34.0	38.0	28.0	38.0
9	35.00187481972887	38.0	35.0	38.0	28.0	38.0
10-14	35.18964522642053	38.0	35.4	38.0	28.8	38.0
15-19	35.17343524661091	38.0	35.2	38.0	28.4	38.0
20-24	35.13262186328238	38.0	35.0	38.0	28.0	38.0
25-29	34.932073839053935	38.0	35.0	38.0	27.2	38.0
30-34	34.85442745889818	38.0	35.0	38.0	27.0	38.0
35-39	34.77998269397173	38.0	35.0	38.0	27.0	38.0
40-44	34.72745889818287	38.0	35.0	38.0	26.2	38.0
45-49	34.667666570522066	38.0	34.8	38.0	25.8	38.0
50-54	34.43507355062013	38.0	34.0	38.0	25.0	38.0
55-59	34.28958753965965	38.0	34.0	38.0	25.0	38.0
60-64	34.13717911739256	38.0	34.0	38.0	24.8	38.0
65-69	34.09936544563023	38.0	34.0	38.0	24.4	38.0
70-74	33.86659936544562	38.0	33.6	38.0	20.4	38.0
75-79	33.741048394380776	38.0	33.6	38.0	18.6	38.0
80-84	33.384505544521254	37.0	33.0	38.0	15.6	38.0
85-89	33.206700926502975	37.0	32.4	38.0	15.0	38.0
90-94	33.045949055270185	37.0	31.6	38.0	15.0	38.0
95-99	32.74468622315929	37.0	31.0	38.0	15.0	38.0
100-104	32.54530665301901	37.0	30.6	38.0	15.0	38.0
105-109	32.176324276201555	36.2	29.0	38.0	15.0	38.0
110-114	31.9778004254116	36.0	29.0	38.0	15.0	38.0
115-119	31.809617230307957	35.0	28.6	38.0	15.0	38.0
120-124	32.25142296529593	35.0	30.6	38.0	16.0	38.0
125-129	31.416486887754946	33.6	30.4	35.4	20.0	37.6
130-134	32.12005469462169	35.8	30.4	37.0	16.0	38.0
135-139	32.851169857186264	37.2	30.4	38.0	16.0	38.0
140-144	32.745305378304465	37.0	29.0	38.0	16.0	38.0
145-149	32.708113035551506	37.0	29.0	38.0	16.0	38.0
150-154	32.69084594837648	37.0	29.0	38.0	16.0	38.0
155-159	32.707916729980255	37.0	29.0	38.0	16.0	38.0
160-164	32.70445221091019	37.0	29.0	38.0	16.0	38.0
165-169	32.61078863394621	37.0	29.0	38.0	16.0	38.0
170-174	32.611536951960346	37.0	29.0	38.0	16.0	38.0
175-179	32.48566228689971	37.0	29.0	38.0	16.0	38.0
180-184	32.39311639602206	37.0	28.8	38.0	16.0	38.0
185-189	32.2330018639064	37.0	28.4	38.0	16.0	38.0
190-194	32.13175765879576	37.0	28.4	38.0	15.4	38.0
195-199	31.81425301394433	37.0	27.4	38.0	15.0	38.0
200-204	31.73655001886337	37.0	27.2	38.0	15.0	38.0
205-209	31.47610286572341	37.0	26.2	38.0	15.0	38.0
210-214	31.317414898108137	36.2	26.0	38.0	15.0	38.0
215-219	31.151628834583647	36.0	25.6	38.0	15.0	38.0
220-224	30.985462906229607	35.8	25.0	38.0	15.0	38.0
225-229	30.829561248797678	35.0	24.6	38.0	15.0	38.0
230-234	30.86815301961176	35.0	24.8	38.0	15.0	38.0
235-239	30.860956440695258	35.0	24.6	38.0	14.2	38.0
240-244	31.190951913166266	35.0	26.2	38.0	14.4	38.0
245-249	32.130674788817956	36.0	29.8	38.0	16.2	38.0
250-252	34.80536657794426	36.666666666666664	33.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	5.0
18	10.0
19	12.0
20	11.0
21	33.0
22	54.0
23	117.0
24	142.0
25	201.0
26	260.0
27	279.0
28	332.0
29	341.0
30	357.0
31	401.0
32	462.0
33	512.0
34	606.0
35	819.0
36	980.0
37	999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.91994807442666	26.81378912447714	12.433290062022213	25.832972739073995
2	15.935967695413902	20.0461494087107	37.16469570233632	26.853187193539085
3	14.046726276319585	29.492356504182286	33.97750216325353	22.483415056244592
4	13.051629650995098	18.589558696279205	42.2843957311797	26.074415921546006
5	11.912316123449667	34.294779348139606	39.3712143063167	14.42169022209403
6	24.617825209114507	42.67378136717623	20.86818575137006	11.840207672339197
7	22.065186039803866	33.58811652725699	28.511681569079894	15.835015863859244
8	21.372944909143353	36.35708104989905	24.358234785116817	17.911739255840782
9	21.2575713873666	17.378136717623306	25.454283241995963	35.91000865301414
10-14	22.050764349581772	24.32362272858379	31.675800403807326	21.949812518027112
15-19	21.442169022209402	29.036631093164118	28.329968272281512	21.191231612344964
20-24	20.98932794923565	27.84539948081915	29.215460051918086	21.949812518027112
25-29	21.30660513412172	28.130948947216616	28.840496106143643	21.721949812518027
30-34	21.03547735794635	28.04153446783963	29.48947216613787	21.433516008076147
35-39	20.954715892702623	27.415633112200748	29.036631093164118	22.593019901932507
40-44	20.276896452264207	26.781078742428612	30.27401211421979	22.668012691087394
45-49	18.91548889529853	26.683011248918376	31.168156907989612	23.23334294779348
50-54	19.247187770406693	26.801269108739547	30.262474762042114	23.689068358811653
55-59	18.50591289299106	26.0888376117681	30.023074704355352	25.382174790885493
60-64	18.996250360542255	26.26189789443323	28.33573694837035	26.40611479665417
65-69	19.417363715027403	25.86674358234785	27.355062013267954	27.360830689356792
70-74	19.72769492601033	26.714166209940288	26.402630743935156	27.15550812011423
75-79	20.38778925500606	25.918979744936234	26.36909227306827	27.32413872698944
80-84	19.460801293153214	25.81399376515414	26.743447638840777	27.981757302851868
85-89	19.891385984170086	24.446819573632215	27.803454850077998	27.85833959211971
90-94	19.593088877955605	23.575376957138307	28.344861517089686	28.4866726478164
95-99	19.538162513417078	23.42258710220185	28.760407298888925	28.27884308549215
100-104	19.50319581638582	24.70947123765253	28.631609529343404	27.155723416618244
105-109	18.805657412257727	27.59734590536057	27.50130958617077	26.09568709621093
110-114	18.403243684732512	29.207747505979814	26.798319817980282	25.590688991307392
115-119	18.584847686770257	30.091007520557167	26.567758171655985	24.756386621016592
120-124	18.95680282104026	29.620922715251247	26.761680869820747	24.660593593887747
125-129	20.54439794044142	27.436692662069795	28.83683117039535	23.182078227093434
130-134	19.92403524764509	31.975083561227592	28.37739288969918	19.723488301428137
135-139	21.93558189000304	25.59708295350957	29.234275296262535	23.233059860224856
140-144	21.011850501367366	28.356122759039803	27.696748708599213	22.93527803099362
145-149	21.030082041932545	27.9519902765117	29.358857490124585	21.659070191431177
150-154	20.979063478075908	27.70974505454435	28.970798261873654	22.340393205506093
155-159	20.361647166084182	27.646254368636985	29.38763105910956	22.604467406169277
160-164	20.364686217900015	27.080990730891962	29.30861571189789	23.245707339310133
165-169	20.28870992250418	27.51861419237198	28.795015955022034	23.397659930101806
170-174	20.529418915633357	26.79613420860686	29.163627522489666	23.51081935327012
175-179	21.000425972129253	27.055315523641454	27.83727864662569	24.1069798576036
180-184	21.082612404045328	26.352503960034117	28.487876203241136	24.07700743267942
185-189	21.345983709082034	25.864120320937182	29.015528234540405	23.774367735440375
190-194	21.302806138044875	25.915510179128205	29.00593018279636	23.775753500030568
195-199	21.23644384535261	25.90527541204583	29.327247104956804	23.531033637644754
200-204	22.178114229443622	25.73239783357952	28.474273756770064	23.615214180206795
205-209	22.60710180496443	24.303333436888376	28.55944577340085	24.53011898474634
210-214	22.844408173514548	25.09494962177093	28.045450265231175	24.015191939483348
215-219	22.65983188996434	25.5285277636271	27.81456953642384	23.997070809984717
220-224	23.053658536585367	26.091056910569105	27.947967479674794	22.907317073170734
225-229	22.302880272973606	27.474659619308866	27.892817716522263	22.32964239119526
230-234	22.269904403042357	27.667294675877468	28.05805596259856	22.004744958481616
235-239	22.977141795205352	27.60825125441368	27.704887567366658	21.70971938301431
240-244	22.614336420382685	27.208192240218676	27.444820692750195	22.732650646648445
245-249	22.62906309751434	27.86806883365201	27.93499043977055	21.567877629063098
250-252	21.006871406534845	28.747721217220583	28.649558266722757	21.595849109521804
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.3333333333333333
19	1.0
20	0.3333333333333333
21	0.3333333333333333
22	0.6666666666666666
23	2.333333333333333
24	4.999999999999999
25	4.333333333333333
26	6.666666666666664
27	10.666666666666668
28	14.666666666666675
29	16.66666666666668
30	29.999999999999968
31	37.66666666666664
32	46.50000000000003
33	63.50000000000014
34	72.83333333333343
35	83.66666666666663
36	97.3333333333331
37	123.83333333333272
38	155.83333333333314
39	168.50000000000003
40	177.16666666666686
41	194.00000000000077
42	193.00000000000082
43	209.50000000000097
44	214.50000000000097
45	210.33333333333434
46	183.16666666666708
47	169.16666666666674
48	152.33333333333286
49	133.83333333333275
50	122.83333333333289
51	99.8333333333332
52	93.33333333333323
53	85.33333333333331
54	74.66666666666681
55	58.00000000000007
56	52.50000000000004
57	45.66666666666668
58	46.499999999999964
59	46.66666666666662
60	33.16666666666664
61	23.333333333333336
62	19.666666666666675
63	23.166666666666664
64	21.16666666666667
65	9.166666666666664
66	8.83333333333333
67	9.666666666666664
68	6.166666666666666
69	2.333333333333333
70	2.333333333333333
71	2.6666666666666665
72	1.5
73	0.8333333333333333
74	0.3333333333333333
75	0.0
76	0.5
77	1.0
78	0.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014421690222094028
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.008653014133256419
75-79	0.008655260956117827
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.012042751768779166
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	3.0
80-84	5.0
85-89	7.0
90-94	18.0
95-99	14.0
100-104	10.0
105-109	13.0
110-114	21.0
115-119	23.0
120-124	56.0
125-129	182.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	3.0
175-179	10.0
180-184	9.0
185-189	10.0
190-194	15.0
195-199	17.0
200-204	53.0
205-209	63.0
210-214	72.0
215-219	118.0
220-224	152.0
225-229	218.0
230-234	287.0
235-239	433.0
240-244	558.0
245-249	1270.0
250-253	3293.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.8704932218056
#Duplication Level	Percentage of total
1	95.05336025382175
2	2.8554946639746177
3	0.5191808479953851
4	0.4614940871070089
5	0.1442169022209403
6	0.17306028266512835
7	0.1009518315546582
8	0.0
9	0.0
>10	0.6922411306605134
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGATTCATTCTTGGCCCGCTGGGTTGCATCCCGTCGCCTCCTGCCGTGCT	26	0.37496394577444475	No Hit
GGTTTTTTTGGTTTTCGTTGTCTAGATGTCCTTTTCCGAGCCCACGAGAC	22	0.31727718488606865	No Hit
GCCCTTTGCTTAATCCTGCTGCTTCCATCTCTCTTGCTTGAGCGCTATAG	7	0.1009518315546582	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.04326507066628209	0.0	0.0
30-34	0.0	0.0	0.0	1.156619555811941	0.19613498702047882	0.0
35-39	0.0	0.0	0.0	3.69483703490049	0.23074704355350448	0.0
40-44	0.0	0.0	0.0	8.808768387655032	0.23074704355350448	0.0
45-49	0.0	0.0	0.0	13.813094894721663	0.2422843957311797	0.0
50-54	0.0	0.0	0.0	18.33862128641477	0.2567060859532737	0.0
55-59	0.0	0.0	0.0	22.639169310643204	0.30862417075281223	0.0
60-64	0.0	0.0	0.0	26.472454571675804	0.3634265935967695	0.0
65-69	0.0	0.0	0.0	30.429766368618402	0.400922988174214	0.0
70-74	0.0	0.0	0.0	34.009229881742144	0.5018748197288723	0.0
75-79	0.0	0.0	0.0	37.33775598500144	0.5509085664839919	0.0
80-84	0.0	0.0	0.0	40.34323622728583	0.6316700317277185	0.0
85-89	0.0	0.0	0.0	43.49870204788	0.7268531871935391	0.0
90-94	0.0	0.0	0.0	46.55610037496395	0.7903086241707529	0.0
95-99	0.0	0.0	0.0	49.192385347562734	0.9576002307470436	0.0
100-104	0.0	0.0	0.0	51.638304009229884	1.3152581482549754	0.0
105-109	0.0	0.0	0.0	53.88808768387655	1.9094317854052494	0.0
110-114	0.0	0.0	0.0	55.797519469281795	2.3738102105566776	0.0
115-119	0.0	0.0	0.0	56.648399192385355	2.737236804153447	0.0
120-124	0.0	0.0	0.0	56.66282088260745	2.7689645226420536	0.0
125-129	0.0	0.0	0.0	56.66282088260745	2.7689645226420536	0.0
130-134	0.0	0.0	0.0	56.691664263051635	2.7689645226420536	0.0
135-139	0.0	0.0	0.0	56.73204499567349	2.7689645226420536	0.0
140-144	0.0	0.0	0.0	56.73781367176233	2.7689645226420536	0.0
145-149	0.0	0.0	0.0	56.7637727141621	2.7689645226420536	0.0
150-154	0.0	0.0	0.0	56.80703778482838	2.7689645226420536	0.0
155-159	0.0	0.0	0.0	56.85030285549467	2.7689645226420536	0.0
160-164	0.0	0.0	0.0	56.896452264205365	2.7689645226420536	0.0
165-169	0.0	0.0	0.0	56.971445053360256	2.7689645226420536	0.0
170-174	0.0	0.0	0.0	57.02336313815979	2.7689645226420536	0.0
175-179	0.0	0.0	0.0	57.04643784251514	2.7689645226420536	0.0
180-184	0.0	0.0	0.0	57.132967983847706	2.7689645226420536	0.0
185-189	0.0	0.0	0.0	57.25122584366888	2.7689645226420536	0.0
190-194	0.0	0.0	0.0	57.36371502740121	2.7689645226420536	0.0
195-199	0.0	0.0	0.0	57.45312950677819	2.7689645226420536	0.0
200-204	0.0	0.0	0.0	57.574271704643785	2.7805018748197288	0.0
205-209	0.0	0.0	0.0	57.67233919815402	2.8554946639746177	0.0
210-214	0.0	0.0	0.0	57.78194404384194	2.869916354196712	0.0
215-219	0.0	0.0	0.0	57.81655610037497	2.910297086818575	0.0
220-224	0.0	0.0	0.0	57.87712719930776	3.0285549466397463	0.0
225-229	0.0	0.0	0.0	57.99826939717335	3.1439284684164988	0.0
230-234	0.0	0.0	0.0	58.08479953850591	3.201615229304875	0.0
235-239	0.0	0.0	0.0	58.21171041246034	3.2391116238823194	0.0
240-241	0.0	0.0	0.0	58.30689356792616	3.2448802999711566	0.0
>>END_MODULE
