##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665172
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104464
Total Bases	25.9 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.37683795374483	30.0	18.0	33.0	18.0	33.0
2	27.906599402665034	29.0	25.0	33.0	18.0	33.0
3	29.909260606524736	31.0	29.0	33.0	25.0	33.0
4	31.236071756777456	33.0	31.0	33.0	29.0	33.0
5	31.391752182570073	33.0	32.0	33.0	28.0	33.0
6	35.01451217644356	37.0	34.0	38.0	29.0	38.0
7	35.67713279215806	38.0	36.0	38.0	31.0	38.0
8	36.189854878235565	38.0	37.0	38.0	33.0	38.0
9	36.43875402052382	38.0	37.0	38.0	34.0	38.0
10-14	36.512710598866605	38.0	37.6	38.0	34.0	38.0
15-19	36.46918172767652	38.0	37.8	38.0	33.8	38.0
20-24	36.454309618624606	38.0	37.6	38.0	33.8	38.0
25-29	36.38941644968602	38.0	37.2	38.0	33.2	38.0
30-34	36.3407068463777	38.0	37.0	38.0	33.0	38.0
35-39	36.27922729361311	38.0	37.0	38.0	33.0	38.0
40-44	36.27120730586613	38.0	37.0	38.0	33.0	38.0
45-49	36.21009534385051	38.0	37.0	38.0	33.0	38.0
50-54	36.07625976412927	38.0	37.0	38.0	32.2	38.0
55-59	35.92987440649411	38.0	37.0	38.0	31.0	38.0
60-64	35.88687777607597	38.0	37.0	38.0	31.0	38.0
65-69	35.82133175065094	38.0	36.8	38.0	31.0	38.0
70-74	35.71810575892173	38.0	36.0	38.0	30.2	38.0
75-79	35.63190469346114	38.0	36.0	38.0	29.4	38.0
80-84	35.51052474528346	38.0	36.0	38.0	29.0	38.0
85-89	35.38194121617396	38.0	36.0	38.0	29.0	38.0
90-94	35.27620840120047	38.0	36.0	38.0	29.0	38.0
95-99	35.166250682283575	38.0	35.0	38.0	28.0	38.0
100-104	35.023272576022784	38.0	35.0	38.0	27.6	38.0
105-109	34.82310317002899	38.0	35.0	38.0	27.0	38.0
110-114	34.70250297941199	38.0	34.8	38.0	26.2	38.0
115-119	34.57610845678439	38.0	34.0	38.0	25.6	38.0
120-124	34.75434797183182	38.0	34.0	38.0	27.4	38.0
125-129	33.234865757588764	34.2	32.8	35.6	30.0	35.6
130-134	34.686924642798566	36.2	35.4	37.0	30.6	37.2
135-139	35.98658022440257	38.0	37.0	38.0	31.0	38.0
140-144	35.91044735801184	38.0	37.0	38.0	31.0	38.0
145-149	35.868555661678876	38.0	37.0	38.0	31.0	38.0
150-154	35.856442167616954	38.0	37.0	38.0	31.0	38.0
155-159	35.841287758193204	38.0	37.0	38.0	31.0	38.0
160-164	35.807364958081294	38.0	37.0	38.0	30.6	38.0
165-169	35.78171676117244	38.0	37.0	38.0	30.6	38.0
170-174	35.73457467848839	38.0	37.0	38.0	29.0	38.0
175-179	35.67935088388913	38.0	37.0	38.0	29.0	38.0
180-184	35.57987516513906	38.0	36.8	38.0	29.0	38.0
185-189	35.5254738529271	38.0	36.0	38.0	29.0	38.0
190-194	35.46968190679322	38.0	36.0	38.0	29.0	38.0
195-199	35.398780509516584	38.0	36.0	38.0	29.0	38.0
200-204	35.30493451027628	38.0	36.0	38.0	28.4	38.0
205-209	35.21394653234014	38.0	36.0	38.0	28.0	38.0
210-214	35.12027717667009	38.0	35.8	38.0	27.6	38.0
215-219	34.97873467492741	38.0	35.0	38.0	27.0	38.0
220-224	34.865734290690725	38.0	35.0	38.0	26.0	38.0
225-229	34.70501776856703	38.0	35.0	38.0	25.0	38.0
230-234	34.59704823509095	38.0	35.0	38.0	24.4	38.0
235-239	34.49731458439311	38.0	34.2	38.0	24.0	38.0
240-244	34.47887015721814	38.0	34.0	38.0	24.0	38.0
245-249	34.694346795521255	38.0	34.0	38.0	26.4	38.0
250-252	35.780940996268576	37.666666666666664	34.333333333333336	38.0	31.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	4.0
17	6.0
18	12.0
19	16.0
20	21.0
21	65.0
22	151.0
23	293.0
24	507.0
25	680.0
26	972.0
27	1280.0
28	1695.0
29	2166.0
30	2634.0
31	3249.0
32	4056.0
33	5372.0
34	7342.0
35	11751.0
36	22570.0
37	39619.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.81801204761585	26.85430812400042	12.006435610377423	24.321244218006303
2	16.172078419359778	20.836843314443254	36.11387655077347	26.877201715423492
3	16.56934446316434	27.511870117935366	31.56015469444019	24.358630724460102
4	14.153201102772247	20.38884208914076	38.47066932148874	26.987287486598255
5	15.200451830295606	34.47694899678358	34.859856026956656	15.462743145964161
6	27.304143054066472	39.314979323020374	19.837455965691532	13.543421657221627
7	24.3615025271864	31.094922652779903	26.488551079797823	18.05502374023587
8	23.543038750191453	34.30942717108286	21.70125593505897	20.446278143666717
9	23.362115178434674	15.517307397763824	21.86207688773166	39.25850053606984
10-14	23.82179506815745	23.02419972430694	28.957152703323636	24.19685250421198
15-19	23.47641292694134	27.808623066319498	25.60365293306785	23.111311073671313
20-24	23.401261300587	26.53498443476962	26.441363307410427	23.62239095723295
25-29	23.727836820027605	26.302700074092648	26.356881522594428	23.612581583285312
30-34	23.51834124674529	26.40450298667483	26.468065553683566	23.60909021289631
35-39	23.454204319191298	26.31413692755399	26.637693368050236	23.59396538520447
40-44	23.418402511870116	26.130533006586	26.637884821565322	23.813179659978555
45-49	22.991122283508737	25.93147099760874	27.239866824679936	23.837539894202585
50-54	22.844512346884464	25.983787654264262	27.038700561726763	24.13299943712451
55-59	22.437140663619985	25.97330739761486	27.26428586471434	24.325266074050816
60-64	22.65697393914795	25.919749730048476	26.803314468635847	24.61996186216773
65-69	22.71099861765798	25.829500940069156	26.493867532576935	24.965632909695923
70-74	22.404757665052568	26.007279717695535	26.496870410685595	25.091092206566294
75-79	22.682127764682907	25.81277034103899	26.20970135393111	25.295400540346996
80-84	22.333273978084513	25.80747071967282	26.38742894123639	25.471826361006283
85-89	22.438131419511485	25.56909136865325	26.417941313888736	25.574835897946524
90-94	22.48871542573055	25.089194577276114	26.734434756297244	25.68765524069609
95-99	22.395475204989072	25.220216011844453	26.67702226197613	25.70728652119034
100-104	22.163478207566868	25.043531230425316	27.00258026699155	25.790410295016276
105-109	22.028643962255114	25.63031086841979	26.62394022129616	25.71710494802893
110-114	21.871999540049252	26.1177282266024	26.513285868971533	25.496986364376816
115-119	21.74945747609444	26.42148931438781	26.559133188660294	25.269920020857455
120-124	21.68169089723426	26.517319421535156	26.432544401396296	25.368445279834283
125-129	23.077663992849274	25.024657682023776	27.81906655262489	24.07861177250206
130-134	21.60738855322294	30.988774756033788	27.633744816260688	19.77009187448259
135-139	24.192465325213604	22.747007201241114	28.023672247049653	25.03685522649563
140-144	23.310789298497813	26.77845420602225	26.160985634207755	23.74977086127218
145-149	23.250779071674593	26.690079016681302	26.620034925566095	23.439106986078013
150-154	23.451785073382705	26.361429602386533	26.483959231401542	23.70282609282922
155-159	23.470705035804635	26.191124001165505	26.68125481206185	23.65691615096801
160-164	23.30524418647283	26.218809758582562	26.731129554194943	23.744816500749664
165-169	23.26726177382069	26.24028898588647	26.6953028215489	23.797146418743946
170-174	23.144253108670316	26.353072392846393	26.661430875985086	23.841243622498208
175-179	23.224234050477314	26.047976411244022	26.614152867643227	24.113636670635433
180-184	23.25530235636698	26.119568594381075	26.635958573019202	23.989170476232747
185-189	23.25693145831283	25.829777625964674	26.982849622043265	23.930441293679237
190-194	23.1302652783541	25.81971879915466	26.894921207793338	24.155094714697906
195-199	22.910469513383923	25.843214997866987	27.186915472920408	24.05940001582868
200-204	23.185797646586316	25.625909949293863	26.866916657333856	24.32137574678597
205-209	23.28461920469016	25.591632222821247	26.968806339599894	24.154942232888704
210-214	23.525622175447285	25.58735219463877	26.59664613384511	24.29037949606884
215-219	23.410841593946138	25.53643909114522	26.81865935778501	24.23405995712364
220-224	23.395071325844444	25.533486220701985	27.132778792708052	23.938663660745522
225-229	23.224562519637097	25.951606387691417	26.962489974103427	23.861341118568056
230-234	23.327157085623227	26.010779287891083	26.817359251305167	23.844704375180523
235-239	23.710862908658097	25.633118270168875	27.021060558793312	23.63495826237971
240-244	23.628096613741402	25.65353556006778	26.89207983529264	23.826287990898177
245-249	23.216900185566658	26.025936771140127	26.90269667512215	23.854466368171064
250-252	22.855315619597693	25.916081260679704	26.841405773785137	24.387197345937466
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.3333333333333333
16	0.3333333333333333
17	2.333333333333333
18	5.333333333333332
19	4.999999999999999
20	7.666666666666663
21	12.666666666666671
22	18.333333333333336
23	28.666666666666632
24	43.500000000000014
25	77.83333333333339
26	108.9999999999996
27	144.49999999999963
28	187.50000000000085
29	254.00000000000267
30	319.99999999999966
31	382.4999999999961
32	481.6666666666572
33	594.9999999999964
34	750.5000000000135
35	858.6666666666925
36	987.0000000000402
37	1161.0000000000198
38	1364.6666666666401
39	1548.1666666666008
40	1716.166666666564
41	1885.9999999998577
42	2056.6666666664864
43	2143.4999999998395
44	2293.9999999999077
45	2443.666666666643
46	2552.5000000000264
47	2617.8333333333876
48	2664.8333333334103
49	2586.1666666667106
50	2542.000000000026
51	2362.4999999999454
52	2226.1666666665487
53	1974.3333333331693
54	1766.9999999998827
55	1553.3333333332653
56	1356.3333333333087
57	1148.0000000000214
58	1018.1666666667114
59	899.5000000000314
60	774.5000000000172
61	618.8333333333326
62	503.16666666665606
63	396.9999999999953
64	306.3333333333337
65	252.50000000000267
66	166.83333333333363
67	127.99999999999932
68	107.99999999999962
69	80.16666666666664
70	52.000000000000085
71	31.333333333333293
72	19.500000000000004
73	14.000000000000005
74	14.000000000000007
75	7.666666666666663
76	2.5
77	2.833333333333333
78	4.333333333333334
79	2.6666666666666665
80	0.6666666666666666
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04307704089447082
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	3.82907030173074E-4
25-29	1.91453515086537E-4
30-34	0.0
35-39	0.0
40-44	0.0
45-49	1.91453515086537E-4
50-54	3.82907030173074E-4
55-59	5.74360545259611E-4
60-64	7.65814060346148E-4
65-69	0.0034461632715576656
70-74	0.008998315209067239
75-79	0.010338667958361972
80-84	9.573335579876084E-4
85-89	0.0
90-94	3.83013357590846E-4
95-99	0.0
100-104	0.0011493262075108468
105-109	5.747919253230331E-4
110-114	1.916457772769435E-4
115-119	5.751103732658026E-4
120-124	5.753927535036623E-4
125-129	0.013482851738710036
130-134	0.011191185976286263
135-139	0.0040519811293450265
140-144	0.0038590296469952633
145-149	0.0038590296469952633
150-154	0.004437884094044552
155-159	0.006946253364591474
160-164	0.006560350399891948
165-169	0.006174530832133802
170-174	0.005209790547124485
175-179	0.007525296573867539
180-184	0.0019296712033236656
185-189	0.00270168565887359
190-194	0.0028949146000192996
195-199	0.002123314377926796
200-204	0.0015447176449234787
205-209	0.0
210-214	0.0011607616143872533
215-219	9.691669234959014E-4
220-224	0.0015547626266159812
225-229	7.805989926369999E-4
230-234	3.9297285932947043E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	5.0
80-84	7.0
85-89	10.0
90-94	18.0
95-99	8.0
100-104	20.0
105-109	29.0
110-114	26.0
115-119	37.0
120-124	117.0
125-129	534.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	2.0
170-174	0.0
175-179	3.0
180-184	7.0
185-189	4.0
190-194	18.0
195-199	18.0
200-204	68.0
205-209	91.0
210-214	174.0
215-219	235.0
220-224	350.0
225-229	551.0
230-234	942.0
235-239	1435.0
240-244	2888.0
245-249	11869.0
250-253	84998.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.42529483841324
#Duplication Level	Percentage of total
1	57.156532393934754
2	22.18754786337877
3	8.962896308776228
4	4.24260989431766
5	2.4697503446163274
6	1.568004288558738
7	0.9716265890641753
8	0.735181497932302
9	0.3187701026190841
>10	1.3870807168019603
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-14	0.0	5.74360545259611E-4	0.0	0.003254709756471129	3.82907030173074E-4	0.0
15-19	0.0	9.57267575432685E-4	0.0	0.004786337877163425	9.57267575432685E-4	0.0
20-24	0.0	9.57267575432685E-4	0.0	0.005935058967682647	9.57267575432685E-4	0.0
25-29	0.0	9.57267575432685E-4	0.0	0.02603767805176903	9.57267575432685E-4	0.0
30-34	0.0	9.57267575432685E-4	0.0	0.24372032470516158	0.006892326543115332	0.0
35-39	0.0	0.00191453515086537	0.0	0.7432225455659365	0.00765814060346148	0.0
40-44	0.0	0.00191453515086537	0.0	1.8339332210139379	0.00765814060346148	0.0
45-49	0.0	0.00191453515086537	0.0	3.1149486904579566	0.007849594118548017	0.0
50-54	0.0	0.00191453515086537	0.0	4.449188237096033	0.012827385510797979	0.0
55-59	0.0	0.00191453515086537	3.82907030173074E-4	5.8787716342472045	0.017039362842701793	0.0
60-64	0.0	0.00191453515086537	9.57267575432685E-4	7.380150099555829	0.02546331750650942	0.0
65-69	0.0	0.00191453515086537	9.57267575432685E-4	8.935135549088681	0.02871802726298055	0.0
70-74	0.0	0.00191453515086537	9.57267575432685E-4	10.495481697043958	0.03005820186858631	0.0
75-79	0.0	0.00191453515086537	9.57267575432685E-4	11.948805330065857	0.031398376474192066	0.0
80-84	0.0	0.00191453515086537	9.57267575432685E-4	13.486559963240925	0.0331214581099709	3.82907030173074E-4
85-89	0.0	0.003637616786644203	9.57267575432685E-4	15.094195129422577	0.034653086230663196	9.57267575432685E-4
90-94	0.0	0.00382907030173074	9.57267575432685E-4	16.65511563792311	0.038673610047480475	9.57267575432685E-4
95-99	0.0	0.00382907030173074	9.57267575432685E-4	18.24417981314137	0.06662582325011487	9.57267575432685E-4
100-104	0.0	0.00382907030173074	9.57267575432685E-4	19.787678051769028	0.11850972583856641	9.57267575432685E-4
105-109	0.0	0.00382907030173074	9.57267575432685E-4	21.402779905039058	0.23089293919436357	9.57267575432685E-4
110-114	0.0	0.00382907030173074	9.57267575432685E-4	22.971167100627966	0.3612727829682953	9.57267575432685E-4
115-119	0.0	0.00382907030173074	9.57267575432685E-4	23.91465002297442	0.45068157451370805	9.57267575432685E-4
120-124	0.0	0.00382907030173074	9.57267575432685E-4	23.9362842701792	0.4575739010568234	9.57267575432685E-4
125-129	0.0	0.00382907030173074	9.57267575432685E-4	23.936475723694286	0.4575739010568234	9.57267575432685E-4
130-134	0.0	0.00382907030173074	9.57267575432685E-4	23.936475723694286	0.4575739010568234	9.57267575432685E-4
135-139	0.0	0.00382907030173074	9.57267575432685E-4	23.93743299126972	0.4575739010568234	9.57267575432685E-4
140-144	0.0	0.00382907030173074	9.57267575432685E-4	23.94107060805636	0.4575739010568234	9.57267575432685E-4
145-149	0.0	0.00382907030173074	9.57267575432685E-4	23.945474038903352	0.4575739010568234	9.57267575432685E-4
150-154	0.0	0.00382907030173074	9.57267575432685E-4	23.948920202174914	0.4575739010568234	9.57267575432685E-4
155-159	0.0	0.00382907030173074	9.57267575432685E-4	23.953706540052075	0.4575739010568234	9.57267575432685E-4
160-164	0.0	0.00382907030173074	9.57267575432685E-4	23.960790320110274	0.4575739010568234	9.57267575432685E-4
165-169	0.0	0.00382907030173074	9.57267575432685E-4	23.975915147802112	0.4575739010568234	9.57267575432685E-4
170-174	0.0	0.00382907030173074	9.57267575432685E-4	23.992188696584467	0.4577653545719099	9.57267575432685E-4
175-179	0.0	0.00382907030173074	9.57267575432685E-4	24.00578189615561	0.45853116863225607	9.57267575432685E-4
180-184	0.0	0.00382907030173074	0.001723081635778833	24.024544340634094	0.45853116863225607	9.57267575432685E-4
185-189	0.0	0.00382907030173074	0.00191453515086537	24.049624751110436	0.45853116863225607	9.57267575432685E-4
190-194	0.0	0.00382907030173074	0.00191453515086537	24.073556440496247	0.45853116863225607	9.57267575432685E-4
195-199	0.0	0.00382907030173074	0.00191453515086537	24.091361617399297	0.45853116863225607	9.57267575432685E-4
200-204	0.0	0.00382907030173074	0.00191453515086537	24.113761678664424	0.4596798897227753	9.57267575432685E-4
205-209	0.0	0.00382907030173074	0.00191453515086537	24.132524123142904	0.465997855720631	9.57267575432685E-4
210-214	0.0	0.00382907030173074	0.00191453515086537	24.158178894164497	0.4748047174146118	9.57267575432685E-4
215-219	0.0	0.00382907030173074	0.00191453515086537	24.189194363608514	0.48399448613876556	9.57267575432685E-4
220-224	0.0	0.00382907030173074	0.00191453515086537	24.211977331903814	0.5010338489814672	9.57267575432685E-4
225-229	0.0	0.00382907030173074	0.00191453515086537	24.240312452136617	0.5243911778220248	9.57267575432685E-4
230-234	0.0	0.00382907030173074	0.00191453515086537	24.263861234492264	0.5448767039362842	9.57267575432685E-4
235-239	0.0	0.00382907030173074	0.00191453515086537	24.2918134476949	0.5580869964772553	9.57267575432685E-4
240-241	0.0	0.0038290703017307397	0.0019145351508653699	24.331827232347987	0.5580869964772553	9.572675754326849E-4
>>END_MODULE
