##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665175
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560019
Total Bases	139 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.72996451906096	31.0	18.0	32.0	18.0	33.0
2	26.93839851862169	29.0	25.0	31.0	18.0	33.0
3	26.06542992291333	28.0	18.0	31.0	18.0	33.0
4	30.000357130740205	31.0	29.0	33.0	27.0	33.0
5	31.585314069701205	33.0	32.0	33.0	29.0	33.0
6	35.58264630307186	37.0	36.0	38.0	30.0	38.0
7	35.9030461466486	38.0	36.0	38.0	31.0	38.0
8	36.47876054205304	38.0	37.0	38.0	34.0	38.0
9	36.725086113149736	38.0	38.0	38.0	34.0	38.0
10-14	36.77075777786111	38.0	38.0	38.0	34.2	38.0
15-19	36.70646995905496	38.0	38.0	38.0	34.0	38.0
20-24	36.70601854579934	38.0	38.0	38.0	34.0	38.0
25-29	36.63833119947716	38.0	38.0	38.0	34.0	38.0
30-34	36.60961110248046	38.0	38.0	38.0	34.0	38.0
35-39	36.54740857006637	38.0	38.0	38.0	34.0	38.0
40-44	36.5674250337935	38.0	38.0	38.0	34.0	38.0
45-49	36.51418684008935	38.0	38.0	38.0	34.0	38.0
50-54	36.389738919572366	38.0	37.6	38.0	33.6	38.0
55-59	36.266402389918916	38.0	37.0	38.0	33.0	38.0
60-64	36.23182713443651	38.0	37.0	38.0	33.0	38.0
65-69	36.154271551500926	38.0	37.0	38.0	33.0	38.0
70-74	36.04698340592015	38.0	37.0	38.0	32.2	38.0
75-79	35.93196153605284	38.0	37.0	38.0	31.0	38.0
80-84	35.817113664631584	38.0	37.0	38.0	31.0	38.0
85-89	35.695755325226074	38.0	36.0	38.0	31.0	38.0
90-94	35.6100004939462	38.0	36.0	38.0	30.2	38.0
95-99	35.49598687369301	38.0	36.0	38.0	29.0	38.0
100-104	35.36312044576816	38.0	35.6	38.0	29.0	38.0
105-109	35.185039770858864	38.0	35.0	38.0	28.2	38.0
110-114	35.07666225018247	38.0	35.0	38.0	28.0	38.0
115-119	34.94366107876982	38.0	35.0	38.0	27.8	38.0
120-124	35.09136049941891	38.0	35.0	38.0	28.8	38.0
125-129	33.30350538631734	34.4	32.8	35.6	29.4	35.6
130-134	34.616218843719956	36.2	35.4	37.0	30.4	37.4
135-139	35.81267524286473	38.0	37.0	38.0	30.2	38.0
140-144	35.70399919642985	38.0	37.0	38.0	29.0	38.0
145-149	35.67947201136478	38.0	37.0	38.0	29.0	38.0
150-154	35.65110096284923	38.0	37.0	38.0	29.0	38.0
155-159	35.63966013287607	38.0	37.0	38.0	29.0	38.0
160-164	35.592771358004946	38.0	37.0	38.0	29.0	38.0
165-169	35.555895797753564	38.0	37.0	38.0	29.0	38.0
170-174	35.515097802215706	38.0	37.0	38.0	29.0	38.0
175-179	35.45978440881448	38.0	36.0	38.0	29.0	38.0
180-184	35.32669552878282	38.0	36.0	38.0	28.0	38.0
185-189	35.226240196615805	38.0	36.0	38.0	27.6	38.0
190-194	35.12053567210309	38.0	36.0	38.0	27.0	38.0
195-199	34.98631243574837	38.0	35.6	38.0	26.8	38.0
200-204	34.81121644063909	38.0	35.0	38.0	25.4	38.0
205-209	34.65009704234879	38.0	35.0	38.0	24.8	38.0
210-214	34.477932922265765	38.0	34.8	38.0	23.4	38.0
215-219	34.27178073733845	38.0	34.0	38.0	23.0	38.0
220-224	34.0953407940404	38.0	34.0	38.0	22.6	38.0
225-229	33.86379753740361	38.0	34.0	38.0	21.0	38.0
230-234	33.72082215211518	38.0	34.0	38.0	21.0	38.0
235-239	33.595189876854455	38.0	33.4	38.0	21.0	38.0
240-244	33.63392889165474	38.0	33.0	38.0	21.0	38.0
245-249	34.0342100790722	38.0	33.2	38.0	23.2	38.0
250-252	35.57050036055817	37.666666666666664	34.333333333333336	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	0.0
6	1.0
7	0.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	11.0
17	31.0
18	38.0
19	50.0
20	98.0
21	314.0
22	810.0
23	1580.0
24	2624.0
25	4070.0
26	5796.0
27	7611.0
28	9914.0
29	12233.0
30	14973.0
31	18100.0
32	22741.0
33	29605.0
34	40991.0
35	62573.0
36	116346.0
37	209506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.692284189404816	17.567169166060655	11.420142977366979	20.320403667167557
2	20.110711320232493	19.035382980813015	36.36349026365365	24.490415435300843
3	19.382873673026616	27.872289135112453	30.776497058114515	21.968340133746416
4	13.26285903056168	20.017678097908092	40.91622545824665	25.803237413283576
5	14.103907931037563	36.51902181191575	35.10852387882468	14.26854637822201
6	25.386284295956358	40.2785639670366	21.26889458318081	13.066257153826236
7	23.01099077703276	31.366838388257456	27.527655509227433	18.09451532548235
8	22.19418730893403	34.55169138024628	21.19993000199994	22.05419130881975
9	22.438327544797907	16.38563252770015	22.316009392605554	38.86003053489639
10-14	24.1422461898342	22.957170790068123	28.44616662053695	24.454416399560728
15-19	23.838781518787346	27.49175379525413	25.326562564730388	23.342902121228136
20-24	23.55719266216248	26.34416666488099	26.558804769322965	23.539835903633563
25-29	24.017641138752722	26.394316379088057	25.96982335290206	23.618219129257163
30-34	23.639366802674928	26.257153826236795	26.412078247904073	23.6914011231842
35-39	23.564225958233262	25.907127487656577	26.698999133951766	23.82964742015839
40-44	23.414408542628323	25.702597251859327	26.982777247038026	23.900216958474328
45-49	22.52169242329439	25.822304982471778	27.53194936101278	24.124053233221048
50-54	22.37438242086023	26.003858508038796	27.30265859918016	24.31910047192082
55-59	22.15699677717969	25.63324276293985	27.140511251299987	25.069249208580473
60-64	22.345479850586088	25.87064960909026	26.557362178023126	25.22650836230052
65-69	22.16304654120486	25.882542860602104	26.286193069945202	25.668217528247837
70-74	22.34537960396605	25.839071920450323	25.828356716289967	25.987191759293655
75-79	22.106510296023856	25.615920144959787	25.936493297610113	26.341076261406243
80-84	21.86343776129164	25.507350007661277	26.187936036699856	26.441276194347225
85-89	21.888710335907806	24.9335367384764	26.55365798861079	26.62409493700501
90-94	21.885165030259774	24.592541471001557	26.769416301770434	26.75287719696823
95-99	21.74579063715957	24.26951496765546	27.05302446810487	26.931669927080105
100-104	21.648335354928374	24.601483773350317	27.14712150470981	26.6030593670115
105-109	21.203691296152368	25.72333145731157	26.82432396215796	26.248653284378097
110-114	20.898802502234137	26.644897229669347	26.461983914209114	25.9943163538874
115-119	21.040416123535334	26.789508222516663	26.308870519278255	25.861205134669753
120-124	20.934586795710082	27.061727953344004	26.346789269837295	25.65689598110862
125-129	23.564756450689924	25.244711015115072	27.833663917298075	23.35686861689693
130-134	20.34945988681429	31.502697605863585	28.286021927443606	19.86182057987852
135-139	24.134803565438425	22.499573962756273	27.827568825195577	25.538053646609733
140-144	23.38039689814574	26.478914673894522	25.737210591364963	24.403477836594778
145-149	23.45473324773382	26.313383910950677	26.723874120640783	23.508008720674724
150-154	23.619645768277003	26.191140762766345	26.355240574485673	23.83397289447098
155-159	23.286206162923655	26.084847562911666	26.651026573926345	23.97791970023833
160-164	23.271438525087166	25.94027989359939	26.85137281043857	23.936908770874876
165-169	23.228577825040578	25.918754386040444	26.68983644105377	24.16283134786521
170-174	22.797707736800884	26.038993444884976	26.861800121910573	24.301498696403566
175-179	23.08505374812489	25.7733390213073	26.76872250568409	24.372884724883725
180-184	23.064169551619333	25.495494881354265	26.73431729683553	24.70601827019087
185-189	22.80965276748623	25.27175365929539	27.250541569617127	24.668052003601254
190-194	22.63826708271153	25.33771343295153	27.331261722796114	24.692757761540832
195-199	22.503271810725074	25.338194822191223	27.370936267460877	24.787597099622822
200-204	22.74762070712333	25.037813601399733	27.099544081138465	25.115021610338474
205-209	22.923227918140757	24.735329140605977	27.235357013000044	25.10608592825322
210-214	22.822369262530604	24.957007823801433	27.043150705907575	25.17747220776039
215-219	22.773429859646622	25.049539994801055	26.913458573139685	25.26357157241263
220-224	22.952416572799596	24.92698722862602	27.349502822554705	24.771093376019678
225-229	22.74889852989516	25.258561014462856	27.08427313600256	24.908267319639425
230-234	22.745032794087447	25.584773115273045	26.9781643852608	24.692029705378708
235-239	22.754455378734203	25.477552230409355	27.278089707678028	24.489902683178418
240-244	22.82794960948802	25.280135431254504	26.945072274765085	24.946842684492392
245-249	22.384091929438707	25.833463647415496	27.207268330987677	24.57517609215812
250-252	21.41182607535279	26.152790119562834	27.597511896132886	24.83787190895149
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.5
6	0.5
7	0.0
8	0.0
9	0.8333333333333333
10	0.5
11	0.3333333333333333
12	0.0
13	0.0
14	1.0
15	4.5
16	15.000000000000007
17	34.8333333333333
18	53.5000000000001
19	77.66666666666666
20	95.66666666666642
21	133.99999999999932
22	158.00000000000003
23	199.8333333333346
24	274.00000000000205
25	381.8333333333292
26	547.3333333333254
27	839.0000000000247
28	1324.3333333333128
29	2008.1666666664914
30	2839.8333333334995
31	3685.50000000055
32	4776.833333333476
33	5999.499999999033
34	7517.999999997663
35	9120.6666666653
36	10328.166666667488
37	10700.000000001499
38	10868.833333335137
39	10582.000000001282
40	10411.500000000939
41	10119.833333333712
42	9701.66666666629
43	9374.999999999016
44	8995.499999998307
45	8692.333333331095
46	8583.499999997564
47	8669.333333331022
48	8549.666666664136
49	8463.333333330671
50	8116.16666666386
51	7650.333333330945
52	6998.833333331531
53	6280.833333332177
54	5670.666666666054
55	5243.166666666428
56	4962.166666666678
57	4681.000000000263
58	4464.333333333782
59	4318.166666667242
60	4379.166666667187
61	4283.833333333942
62	4344.000000000551
63	4369.833333333862
64	4217.500000000669
65	4105.166666667396
66	3915.166666667312
67	3676.333333333871
68	3339.1666666670526
69	2973.1666666668875
70	2543.833333333359
71	2022.4999999998236
72	1633.833333333243
73	1275.3333333333255
74	934.8333333333683
75	668.3333333333384
76	433.6666666666599
77	280.0000000000019
78	172.33333333333377
79	106.9999999999996
80	54.33333333333343
81	28.833333333333304
82	14.166666666666673
83	4.833333333333333
84	0.8333333333333333
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06249787953623002
2	7.142614804140574E-4
3	7.142614804140574E-4
4	7.142614804140574E-4
5	7.142614804140574E-4
6	7.142614804140574E-4
7	7.142614804140574E-4
8	5.35696110310543E-4
9	7.142614804140574E-4
10-14	7.142614804140574E-4
15-19	6.785484063933546E-4
20-24	0.0011785314426831948
25-29	0.0017499406270144405
30-34	7.142614804140574E-4
35-39	7.142614804140574E-4
40-44	7.142614804140574E-4
45-49	0.0013928098868074119
50-54	0.0017499406270144405
55-59	0.002249923663304281
60-64	0.003142750513821852
65-69	0.005214108807022619
70-74	0.01196387979693546
75-79	0.012071381148145488
80-84	0.002535837454962098
85-89	7.143655701512347E-4
90-94	0.0012502406713292308
95-99	3.5723905651736216E-4
100-104	0.002286523551728487
105-109	3.930696812490754E-4
110-114	3.9320667550547616E-4
115-119	0.0010013185219251207
120-124	0.001431144933836381
125-129	0.021365070260969315
130-134	0.016466013287606364
135-139	0.008789048486848713
140-144	0.00699536512218571
145-149	0.005775660434214869
150-154	0.008322690812036333
155-159	0.010008753174819555
160-164	0.012376472934806123
165-169	0.009757763520601079
170-174	0.007139044186019815
175-179	0.009722189336587934
180-184	0.004448770435193792
185-189	0.005095177557967513
190-194	0.004306697129371029
195-199	0.003482629219905416
200-204	0.0024430043492662725
205-209	1.7982157384157144E-4
210-214	9.007288698014434E-4
215-219	8.315346876502636E-4
220-224	9.450225842224118E-4
225-229	6.229684814593586E-4
230-234	1.856861250870868E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	33.0
80-84	33.0
85-89	46.0
90-94	37.0
95-99	52.0
100-104	51.0
105-109	181.0
110-114	231.0
115-119	237.0
120-124	391.0
125-129	1215.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	1.0
160-164	3.0
165-169	8.0
170-174	6.0
175-179	22.0
180-184	44.0
185-189	99.0
190-194	165.0
195-199	300.0
200-204	483.0
205-209	776.0
210-214	1482.0
215-219	2505.0
220-224	3718.0
225-229	5908.0
230-234	9406.0
235-239	13529.0
240-244	23730.0
245-249	73503.0
250-253	421824.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.890193349792064
#Duplication Level	Percentage of total
1	47.3645631122095
2	15.670947586386355
3	6.530299299500411
4	4.022648081474014
5	2.9798364792994243
6	2.418540076790441
7	2.001331596138446
8	1.7198763370370191
9	1.4955845757222037
>10	13.570236042322076
>50	1.5497738111044677
>100	0.6763630020156621
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	3.571307402070287E-4	0.0
2	0.0	0.0	0.0	0.0	7.142614804140574E-4	0.0
3	0.0	0.0	0.0	0.0	8.928268505175717E-4	0.0
4	0.0	0.0	0.0	3.571307402070287E-4	0.0012499575907246004	0.0
5	0.0	0.0	0.0	7.142614804140574E-4	0.0017856537010351435	0.0
6	0.0	0.0	0.0	7.142614804140574E-4	0.0017856537010351435	0.0
7	0.0	0.0	0.0	8.928268505175717E-4	0.0019642190711386576	0.0
8	0.0	0.0	0.0	8.928268505175717E-4	0.0019642190711386576	0.0
9	0.0	0.0	0.0	0.001071392220621086	0.0023213498113456863	0.0
10-14	0.0	0.0	0.0	0.0014642360348488177	0.002821332847635526	0.0
15-19	0.0	0.0	0.0	0.001857079849076549	0.0034284551059874753	0.0
20-24	0.0	0.0	0.0	0.0033213158839253665	0.003749872772173801	0.0
25-29	0.0	0.0	0.0	0.04431992485969226	0.004249855808463642	0.0
30-34	0.0	3.571307402070287E-5	0.0	0.5283035039882575	0.005285534955064025	1.7856537010351435E-4
35-39	0.0	1.7856537010351435E-4	0.0	1.6044455634540973	0.005714091843312459	4.285568882484344E-4
40-44	0.0	1.7856537010351435E-4	0.0	3.580485662093608	0.005785517991353866	5.35696110310543E-4
45-49	0.0	1.7856537010351435E-4	0.0	5.73648394072344	0.0059997964354780815	5.35696110310543E-4
50-54	0.0	1.7856537010351435E-4	0.0	7.975693681821511	0.00721404095218198	5.35696110310543E-4
55-59	0.0	1.7856537010351435E-4	0.0	10.167297895249982	0.010856774502293673	5.71409184331246E-4
60-64	0.0	1.7856537010351435E-4	0.0	12.429863986757592	0.023534915779643194	7.142614804140574E-4
65-69	0.0	1.7856537010351435E-4	0.0	14.641503234711681	0.028891876882748618	8.928268505175717E-4
70-74	0.0	1.7856537010351435E-4	0.0	16.881820081104394	0.029713277585224785	8.928268505175717E-4
75-79	0.0	1.7856537010351435E-4	0.0	19.125315391084946	0.030534678287700956	9.999660725796805E-4
80-84	0.0	1.7856537010351435E-4	0.0	21.362560913111878	0.031177513620073604	0.0011785314426831946
85-89	0.0	1.7856537010351435E-4	0.0	23.568021799260382	0.032141766618632585	0.0014285229608281148
90-94	0.0	1.7856537010351435E-4	0.0	25.712770459573694	0.03639162242709622	0.0016428014049523318
95-99	0.0	2.85704592165623E-4	0.0	27.917088527353535	0.08835414512721888	0.0017856537010351435
100-104	0.0	3.571307402070287E-4	0.0	30.03651661818617	0.21367132186586524	0.0017856537010351435
105-109	0.0	3.571307402070287E-4	0.0	32.074590326399644	0.43987793271299724	0.0017856537010351435
110-114	0.0	3.571307402070287E-4	0.0	34.107664204250206	0.6691915809999304	0.0017856537010351435
115-119	0.0	3.571307402070287E-4	0.0	35.32537288913412	0.8249720098782362	0.0017856537010351435
120-124	0.0	3.571307402070287E-4	0.0	35.36040741474843	0.8408643278174491	0.0018213667750558462
125-129	0.0	3.571307402070287E-4	0.0	35.360800258562655	0.8411143193355939	0.0019642190711386576
130-134	0.0	3.571307402070287E-4	0.0	35.361550233117086	0.8412214585576561	0.0019642190711386576
135-139	0.0	3.571307402070287E-4	0.0	35.36358587833627	0.8412214585576561	0.0019642190711386576
140-144	0.0	3.571307402070287E-4	0.0	35.36658577655401	0.8416500154459046	0.0019642190711386576
145-149	0.0	3.571307402070287E-4	0.0	35.37147846769484	0.8419000069640494	0.0019642190711386576
150-154	0.0	3.571307402070287E-4	0.0	35.37790682101857	0.8422928507782773	0.0019642190711386576
155-159	0.0	3.571307402070287E-4	0.0	35.38794219481839	0.8429713991846706	0.0020356452191800634
160-164	0.0	3.571307402070287E-4	0.0	35.39890610854275	0.8432213907028154	0.002142784441242172
165-169	0.0	3.571307402070287E-4	0.0	35.41065570989556	0.8435428083690019	0.002214210589283578
170-174	0.0	3.571307402070287E-4	0.0	35.42715515009312	0.844007078331271	0.0023213498113456863
175-179	0.0	3.571307402070287E-4	1.071392220621086E-4	35.448297289913384	0.8442927829234366	0.002392775959387092
180-184	0.0	5.35696110310543E-4	1.7856537010351435E-4	35.477010601426024	0.8448999051817886	0.002499915181449201
185-189	0.0	5.35696110310543E-4	1.7856537010351435E-4	35.50475965994011	0.8453998882180782	0.002499915181449201
190-194	0.0	5.35696110310543E-4	2.142784441242172E-4	35.531580178529666	0.8467926981048859	0.002499915181449201
195-199	0.0	5.35696110310543E-4	3.571307402070287E-4	35.55986493315405	0.8487926302500451	0.002499915181449201
200-204	0.0	5.35696110310543E-4	3.571307402070287E-4	35.586864017113705	0.8588280040498626	0.002499915181449201
205-209	0.0	5.35696110310543E-4	3.571307402070287E-4	35.6178986784377	0.8926125720734476	0.002499915181449201
210-214	0.0	5.35696110310543E-4	3.571307402070287E-4	35.648004799837146	0.9394324121145889	0.002499915181449201
215-219	0.0	5.35696110310543E-4	3.571307402070287E-4	35.67421819616835	1.0050730421646408	0.002499915181449201
220-224	0.0	5.35696110310543E-4	3.571307402070287E-4	35.698074529614175	1.106748163901582	0.002499915181449201
225-229	0.0	5.35696110310543E-4	3.571307402070287E-4	35.72450220438949	1.220565730805562	0.002499915181449201
230-234	0.0	5.35696110310543E-4	3.571307402070287E-4	35.754358334270805	1.311562643410313	0.002642767477532012
235-239	0.0	5.35696110310543E-4	3.571307402070287E-4	35.78796433692428	1.3655965244036363	0.002678480551552715
240-241	0.0	5.35696110310543E-4	3.571307402070287E-4	35.84467669846916	1.3678107349929198	0.002678480551552715
>>END_MODULE
