##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665177
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1176504
Total Bases	293.2 Mbp
Sequences flagged as poor quality	0
Sequence length	153-252
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.168064876957494	31.0	18.0	33.0	18.0	33.0
2	28.437106036188574	30.0	27.0	33.0	18.0	33.0
3	30.245765420262064	31.0	29.0	33.0	25.0	33.0
4	31.482719990752262	33.0	31.0	33.0	29.0	33.0
5	31.67684597757424	33.0	32.0	33.0	29.0	33.0
6	35.38403864330253	37.0	35.0	38.0	29.0	38.0
7	35.96434861249941	38.0	36.0	38.0	32.0	38.0
8	36.47708040091661	38.0	37.0	38.0	34.0	38.0
9	36.664755070955984	38.0	38.0	38.0	34.0	38.0
10-14	36.76885722445482	38.0	38.0	38.0	34.2	38.0
15-19	36.75145430869763	38.0	38.0	38.0	34.0	38.0
20-24	36.74562806416297	38.0	38.0	38.0	34.0	38.0
25-29	36.66695837838205	38.0	38.0	38.0	34.0	38.0
30-34	36.63134200988692	38.0	38.0	38.0	34.0	38.0
35-39	36.61157548125634	38.0	38.0	38.0	34.0	38.0
40-44	36.58352270795509	38.0	38.0	38.0	34.0	38.0
45-49	36.53069381829556	38.0	38.0	38.0	34.0	38.0
50-54	36.43675244623053	38.0	38.0	38.0	34.0	38.0
55-59	36.34361430135384	38.0	37.2	38.0	33.2	38.0
60-64	36.28746574597281	38.0	37.0	38.0	33.0	38.0
65-69	36.240879419024495	38.0	37.0	38.0	33.0	38.0
70-74	36.138844576814016	38.0	37.0	38.0	33.0	38.0
75-79	36.009043913152865	38.0	37.0	38.0	31.8	38.0
80-84	35.84206972521981	38.0	37.0	38.0	31.0	38.0
85-89	35.724162603782055	38.0	36.6	38.0	31.0	38.0
90-94	35.611339017971886	38.0	36.0	38.0	29.6	38.0
95-99	35.50936843393647	38.0	36.0	38.0	29.0	38.0
100-104	35.32008645954455	38.0	35.6	38.0	28.6	38.0
105-109	35.14523180541673	38.0	35.0	38.0	28.0	38.0
110-114	35.01436714197317	38.0	35.0	38.0	27.6	38.0
115-119	34.89420707451909	38.0	35.0	38.0	27.4	38.0
120-124	35.0507022500561	38.0	34.4	38.0	28.8	38.0
125-129	33.665733733161986	34.4	33.2	35.6	31.2	35.6
130-134	35.20777217927011	36.2	36.0	37.2	32.8	38.0
135-139	36.421300905054295	38.0	38.0	38.0	34.0	38.0
140-144	36.360806423097586	38.0	38.0	38.0	33.2	38.0
145-149	36.34741658336903	38.0	38.0	38.0	33.0	38.0
150-154	36.331630207128335	38.0	38.0	38.0	33.0	38.0
155-159	36.33378268800223	38.0	38.0	38.0	33.0	38.0
160-164	36.3279479854673	38.0	38.0	38.0	33.0	38.0
165-169	36.299224806828136	38.0	38.0	38.0	33.0	38.0
170-174	36.25729583668287	38.0	37.8	38.0	33.0	38.0
175-179	36.218023686431955	38.0	37.0	38.0	33.0	38.0
180-184	36.14350558547377	38.0	37.0	38.0	33.0	38.0
185-189	36.067306546069005	38.0	37.0	38.0	32.6	38.0
190-194	36.00630463520973	38.0	37.0	38.0	31.4	38.0
195-199	35.90438413373285	38.0	37.0	38.0	31.0	38.0
200-204	35.798966157815435	38.0	37.0	38.0	31.0	38.0
205-209	35.669096877613114	38.0	37.0	38.0	29.6	38.0
210-214	35.5373985598421	38.0	36.4	38.0	29.0	38.0
215-219	35.3645854840499	38.0	36.0	38.0	28.6	38.0
220-224	35.22562445435578	38.0	36.0	38.0	27.8	38.0
225-229	35.03588089653273	38.0	35.0	38.0	27.0	38.0
230-234	34.92236610610398	38.0	35.0	38.0	26.0	38.0
235-239	34.71690452007687	38.0	35.0	38.0	25.0	38.0
240-244	34.65453823244094	38.0	34.6	38.0	25.0	38.0
245-249	34.87044471685976	38.0	34.4	38.0	27.2	38.0
250-252	35.87300050681119	37.666666666666664	34.666666666666664	38.0	31.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	3.0
20	38.0
21	253.0
22	712.0
23	1762.0
24	3117.0
25	5277.0
26	7917.0
27	11230.0
28	15043.0
29	19411.0
30	24905.0
31	31652.0
32	40271.0
33	52946.0
34	74170.0
35	116411.0
36	236547.0
37	534837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.436055266874654	26.19955630723131	12.281480461107156	23.082907964786884
2	16.7895731761218	21.176468588292092	39.52931736738677	22.504640868199346
3	15.102796080591311	30.331813576494426	34.02818859944377	20.537201743470483
4	12.45886116834282	20.490708063890985	42.26249974500724	24.787931022758954
5	12.797406553653875	36.354572530140146	37.38176835777864	13.46625255842734
6	24.062306630491694	43.52641384984666	20.706686930091188	11.704592589570456
7	21.260361205741756	33.220626534206424	29.641123192101343	15.877889067950472
8	20.2409001584355	38.09022323766005	22.03290426551886	19.635972338385592
9	21.83230996239707	16.659611867022978	23.035960778713886	38.47211739186607
10-14	22.678681925433317	24.07475027709213	30.00511685468133	23.241450942793225
15-19	22.109725083807618	29.25270122328526	26.9066148521382	21.73095884076892
20-24	22.026548168597955	27.80230904638577	27.892032574502572	22.2791102105137
25-29	22.22128157699286	27.939220265170157	27.764549245922687	22.074948911914298
30-34	22.12964851798209	27.95876597104642	27.953411123124106	21.958174387847386
35-39	22.017961689888008	27.51035270598315	28.037133745401633	22.43455185872721
40-44	21.74467745116039	27.246401202205856	28.569983612465403	22.43893773416835
45-49	20.960256924383796	27.022517092621534	29.037660630863467	22.97956535213121
50-54	20.69845742613777	27.176427869346842	28.9647983359473	23.16031636856809
55-59	20.307326788688012	26.847727858443086	28.664108330041067	24.180837022827834
60-64	20.378534362954053	26.813057540045133	28.127991432104952	24.680416664895855
65-69	20.470932511720527	26.88001898540293	27.285974771581873	25.363073731294673
70-74	21.109289579397313	26.598921100891225	26.54188371615817	25.749905603553287
75-79	21.192852453152618	26.529575909364304	26.40922949393304	25.868342143550034
80-84	21.46122299937305	25.958188212542517	26.48971790014239	26.090870887942042
85-89	21.519904027248266	25.50047148297247	26.82040281152428	26.15922167825499
90-94	21.673221436178757	24.946854943106526	26.888517352785428	26.491406267929285
95-99	21.467070530412858	24.305075790750617	27.401695058165625	26.8261586206709
100-104	21.390895231515543	24.795884576102225	27.40399588199215	26.409224310390083
105-109	21.15042826142944	25.93932346848834	26.944830476132193	25.965417793950024
110-114	20.922574833493215	27.126424119841708	26.467400811186685	25.48360023547839
115-119	20.775237127716796	27.571753701327534	26.41589845378245	25.237110717173223
120-124	20.65816561579353	27.895777410959116	26.500583944335744	24.945473028911618
125-129	22.448099592196506	26.49877645349707	29.001146692275675	22.051977262030746
130-134	19.009563261948635	33.26242130788481	29.52182330051725	18.20619212964931
135-139	22.85659736319007	23.870222762632025	29.123645263582386	24.14953461059552
140-144	21.87541652272548	28.053179581477966	27.40352313622231	22.667880759574246
145-149	21.885752396372805	27.707565929897033	28.1724834196656	22.234198254064562
150-154	21.88580832500289	27.697839525062733	28.15550387280603	22.260848277128343
155-159	21.82499621722802	27.63033562113116	28.249775158833916	22.2948930028069
160-164	21.703919134381252	27.41219546944978	28.399570332543433	22.484315063625534
165-169	21.675116038244123	27.501705657205488	28.25902935736549	22.564148947184894
170-174	21.54238489747413	27.313262823597018	28.091731795516097	23.05262048341276
175-179	21.705272156205083	27.03892307906789	28.2774032329526	22.978401531774427
180-184	21.788975657210106	26.71900987887174	28.249540500789962	23.242473963128198
185-189	21.34998595638386	26.444452227562348	28.871889577052528	23.333672239001263
190-194	21.418999879107723	26.6266684962038	28.612719465815967	23.341612158872515
195-199	21.48738350003758	26.297880068851594	28.812711356261417	23.40202507484941
200-204	21.669996114887738	25.90961636727715	28.475933736722748	23.94445378111237
205-209	22.018853447853296	25.346766463152274	28.59699997821434	24.03738011078009
210-214	22.1608441577143	25.73522883338676	28.229876501426464	23.874050507472475
215-219	22.259125366424808	26.160295361895237	27.775453170268953	23.805126101411002
220-224	22.657222192064523	25.853441836910683	28.365392874247668	23.12394309677712
225-229	22.20005281433615	26.448907321222954	28.168505420045292	23.182534444395607
230-234	22.76894083122862	26.474888288355263	27.825948663493172	22.93022221692294
235-239	23.0507669094747	26.06238868964299	28.13877550874454	22.74806889213777
240-244	23.049209647638595	26.020574251947735	28.011440556072785	22.918775544340885
245-249	22.393764092876157	26.618319565105995	28.073174918348265	22.914741423669582
250-252	21.551730097029214	27.124492791358385	28.351425022984238	22.972352088628156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.3333333333333333
12	0.6666666666666666
13	1.0
14	2.6666666666666665
15	4.333333333333333
16	9.666666666666666
17	12.000000000000004
18	14.333333333333341
19	29.66666666666663
20	47.66666666666673
21	76.0
22	115.99999999999943
23	204.00000000000142
24	338.66666666666487
25	527.9999999999901
26	766.8333333333505
27	1182.8333333333437
28	1902.9999999998465
29	2962.5000000002296
30	4468.500000000406
31	6268.999999998768
32	8742.999999998021
33	11802.166666670253
34	15044.50000000948
35	18243.33333333849
36	21505.666666659956
37	24577.66666664878
38	27015.499999973246
39	28461.833333301325
40	29159.833333298782
41	28843.9999999666
42	27430.666666638415
43	25878.666666644058
44	24438.499999982643
45	23073.333333320945
46	22021.1666666581
47	21779.666666658984
48	21332.499999993943
49	20731.666666662783
50	20213.333333331324
51	19072.83333333548
52	17577.166666674246
53	15588.500000010456
54	13764.00000000713
55	12059.333333337376
56	10314.000000000875
57	9346.999999999121
58	8552.333333331013
59	7790.666666664057
60	6843.6666666649135
61	5657.499999999325
62	4729.333333333502
63	3902.166666667323
64	3233.1666666670194
65	2656.166666666757
66	2166.833333333201
67	1755.3333333332137
68	1464.3333333332796
69	1128.1666666666895
70	905.6666666666997
71	695.8333333333426
72	545.1666666666588
73	415.49999999999386
74	302.0000000000003
75	201.66666666666802
76	141.33333333333297
77	99.99999999999966
78	64.00000000000017
79	36.999999999999986
80	24.99999999999998
81	17.166666666666675
82	8.83333333333333
83	3.3333333333333335
84	2.333333333333333
85	0.3333333333333333
86	0.3333333333333333
87	0.3333333333333333
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03952387752187838
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	8.499758606855565E-5
25-29	5.609840680524672E-4
30-34	0.0
35-39	0.0
40-44	0.0
45-49	1.8699468935082244E-4
50-54	2.3799324099195584E-4
55-59	8.839748951129787E-4
60-64	0.0016149541353025572
65-69	0.0018019488246533797
70-74	0.007020800609262697
75-79	0.005677838749379518
80-84	0.0010879691016775124
85-89	2.889917926330892E-4
90-94	5.949831024798895E-4
95-99	1.3599613770968905E-4
100-104	0.0010879691016775124
105-109	2.54992758205667E-4
110-114	4.589869647702005E-4
115-119	6.119826196936008E-4
120-124	0.0018699468935082244
125-129	0.02135139362042118
130-134	0.01791749114325153
135-139	0.009587727708533077
140-144	0.008295764400291032
145-149	0.006816806402698164
150-154	0.008142770129599242
155-159	0.009859736745028908
160-164	0.012664676924091666
165-169	0.009621819975748253
170-174	0.00817691929380657
175-179	0.009265003057451008
180-184	0.00518508897612683
185-189	0.005899330893469814
190-194	0.005236690771879719
195-199	0.004438220732612505
200-204	0.0026705181094296678
205-209	6.808014667186798E-5
210-214	4.601203470329906E-4
215-219	5.640559933256792E-4
220-224	7.902017728692123E-4
225-229	3.634000749296345E-4
230-234	2.454809585680745E-4
235-239	0.0
240-244	0.0
245-249	0.0
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
152-153	1.0
154-155	1.0
156-157	0.0
158-159	0.0
160-161	2.0
162-163	0.0
164-165	6.0
166-167	3.0
168-169	5.0
170-171	4.0
172-173	3.0
174-175	6.0
176-177	5.0
178-179	7.0
180-181	9.0
182-183	14.0
184-185	22.0
186-187	21.0
188-189	34.0
190-191	38.0
192-193	64.0
194-195	66.0
196-197	85.0
198-199	117.0
200-201	181.0
202-203	216.0
204-205	301.0
206-207	392.0
208-209	495.0
210-211	695.0
212-213	1046.0
214-215	1403.0
216-217	2044.0
218-219	2271.0
220-221	2547.0
222-223	2849.0
224-225	3274.0
226-227	4308.0
228-229	5857.0
230-231	7030.0
232-233	8005.0
234-235	8939.0
236-237	10032.0
238-239	11421.0
240-241	13618.0
242-243	16657.0
244-245	23110.0
246-247	37719.0
248-249	88788.0
250-251	463305.0
252-253	459488.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.35020778145526
#Duplication Level	Percentage of total
1	36.43409590376152
2	10.091100979512932
3	5.337674690422358
4	3.9820361643125004
5	3.162542200201405
6	2.741613026685402
7	2.426872914370631
8	1.9788424974700034
9	1.7594930281030603
>10	20.125206711524235
>50	5.043057663682189
>100	5.201091962965734
>500	1.3722811740957641
>1k	0.34409108289222456
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATATATACCCCCCTTTACATCGGCAAGGTTCCAAGTAGCGGTGGCAGTG	1735	0.14747081182894406	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	8.499758606855565E-5
4	0.0	0.0	0.0	0.0	0.0	8.499758606855565E-5
5	0.0	0.0	0.0	8.499758606855565E-5	0.0	8.499758606855565E-5
6	0.0	0.0	0.0	8.499758606855565E-5	0.0	8.499758606855565E-5
7	0.0	0.0	0.0	8.499758606855565E-5	0.0	8.499758606855565E-5
8	0.0	0.0	0.0	8.499758606855565E-5	0.0	8.499758606855565E-5
9	0.0	0.0	0.0	8.499758606855565E-5	0.0	8.499758606855565E-5
10-14	0.0	0.0	6.799806885484452E-5	1.8699468935082244E-4	1.699951721371113E-5	8.499758606855565E-5
15-19	0.0	0.0	8.499758606855565E-5	3.739893787016449E-4	8.499758606855565E-5	8.499758606855565E-5
20-24	0.0	0.0	8.499758606855565E-5	0.0017339507557985352	1.0199710328226679E-4	8.499758606855565E-5
25-29	0.0	0.0	8.499758606855565E-5	0.03248607739540197	1.8699468935082244E-4	8.499758606855565E-5
30-34	0.0	0.0	8.499758606855565E-5	0.5350768038187714	8.499758606855566E-4	8.499758606855565E-5
35-39	0.0	0.0	8.499758606855565E-5	1.8536953550519164	0.001019971032822668	1.1899662049597792E-4
40-44	0.0	0.0	8.499758606855565E-5	4.438522945948335	0.0010879691016775124	1.699951721371113E-4
45-49	0.0	0.0	8.499758606855565E-5	7.256923903361144	0.0011049686188912234	1.8699468935082244E-4
50-54	0.0	0.0	1.1899662049597792E-4	10.28633986794775	0.0011049686188912234	2.54992758205667E-4
55-59	0.0	0.0	1.699951721371113E-4	13.31385188660642	0.0011049686188912234	2.54992758205667E-4
60-64	0.0	0.0	1.699951721371113E-4	16.399502254135985	0.0016999517213711127	3.399903442742226E-4
65-69	0.0	0.0	1.699951721371113E-4	19.435089043471166	0.00195494447957678	4.249879303427783E-4
70-74	0.0	0.0	1.699951721371113E-4	22.53484901028811	0.00195494447957678	4.929859991976229E-4
75-79	0.0	0.0	1.699951721371113E-4	25.571370773070043	0.002039942065645336	5.439845508387562E-4
80-84	0.0	8.499758606855565E-5	1.699951721371113E-4	28.65319624922652	0.002736922271407492	5.949831024798896E-4
85-89	0.0	8.499758606855565E-5	1.699951721371113E-4	31.670661553211893	0.0038078918558712926	5.949831024798896E-4
90-94	0.0	8.499758606855565E-5	1.699951721371113E-4	34.620706771927686	0.005847833921516629	5.949831024798896E-4
95-99	0.0	8.499758606855565E-5	1.699951721371113E-4	37.49979600579344	0.056319400529024975	5.949831024798896E-4
100-104	0.0	8.499758606855565E-5	1.699951721371113E-4	40.27716012865235	0.20700312111136046	5.949831024798896E-4
105-109	0.0	8.499758606855565E-5	1.699951721371113E-4	42.99861283939536	0.5124334468901084	6.62981171334734E-4
110-114	0.0	8.499758606855565E-5	1.699951721371113E-4	45.59443911792905	0.8162488185335537	6.799806885484452E-4
115-119	0.0	8.499758606855565E-5	1.699951721371113E-4	47.118989820689094	1.0502981715319284	6.969802057621564E-4
120-124	0.0	8.499758606855565E-5	1.699951721371113E-4	47.163460557720164	1.0731795217015836	7.649782746170008E-4
125-129	0.0	8.499758606855565E-5	1.699951721371113E-4	47.16381754758165	1.0732475197704385	7.649782746170008E-4
130-134	0.0	8.499758606855565E-5	2.0399420656453358E-4	47.164718521993976	1.0733665163909345	7.81977791830712E-4
135-139	0.0	8.499758606855565E-5	2.54992758205667E-4	47.16716645247275	1.0735365115630715	9.179739295404011E-4
140-144	0.0	1.0199710328226679E-4	2.54992758205667E-4	47.171110340466335	1.0736385086663538	9.349734467541122E-4
145-149	0.0	1.699951721371113E-4	2.54992758205667E-4	47.175836206251745	1.0737915043212773	9.349734467541122E-4
150-154	0.0	1.699951721371113E-4	2.54992758205667E-4	47.18486294989223	1.07408049611391	9.349734467541122E-4
155-159	0.0	1.699951721371113E-4	2.54992758205667E-4	47.19745959214758	1.0741144951483377	0.001019971032822668
160-164	0.0	1.699951721371113E-4	2.54992758205667E-4	47.210260228609506	1.0741144951483377	0.001019971032822668
165-169	0.0	1.699951721371113E-4	2.54992758205667E-4	47.22640976996253	1.0741144951483377	0.001019971032822668
170-174	0.0	1.699951721371113E-4	2.54992758205667E-4	47.24468425096727	1.0741144951483377	0.001019971032822668
175-179	0.0	1.699951721371113E-4	2.54992758205667E-4	47.265661655209	1.0743694879065435	0.001019971032822668
180-184	0.0	1.699951721371113E-4	2.54992758205667E-4	47.29459483350673	1.0745394830786805	0.0010709695844638013
185-189	0.0	1.699951721371113E-4	2.54992758205667E-4	47.327811890142314	1.0746074811475352	0.0011049686188912234
190-194	0.0	1.699951721371113E-4	2.54992758205667E-4	47.35949899022867	1.0749474714918097	0.0011049686188912234
195-199	0.0	1.699951721371113E-4	2.54992758205667E-4	47.391050094177324	1.0757634483180678	0.0011049686188912234
200-204	0.0	1.699951721371113E-4	2.54992758205667E-4	47.42183621985136	1.0791973507952375	0.0011049686188912234
205-209	0.0	1.699951721371113E-4	2.54992758205667E-4	47.45129638318272	1.094224924012158	0.0011049686188912234
210-214	0.0	1.699951721371113E-4	2.54992758205667E-4	47.48017856292882	1.1210331626581804	0.0011049686188912234
215-219	0.0	1.699951721371113E-4	2.54992758205667E-4	47.510420704052	1.1499153424042758	0.0011049686188912234
220-224	0.0	1.699951721371113E-4	2.54992758205667E-4	47.54056084807191	1.1922441402664163	0.0011899662049597792
225-229	0.0	1.699951721371113E-4	2.54992758205667E-4	47.56912003699095	1.2381258372262227	0.0012409647566009128
230-234	0.0	1.699951721371113E-4	2.54992758205667E-4	47.6009261336978	1.273008846548758	0.001274963791028335
235-239	0.0	1.699951721371113E-4	2.54992758205667E-4	47.64024601701311	1.294666231479026	0.001274963791028335
240-241	0.0	1.699951721371113E-4	2.54992758205667E-4	47.70765760252409	1.295788199615131	0.001274963791028335
>>END_MODULE
