##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665178
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2047585
Total Bases	507.7 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.61193210538268	32.0	25.0	33.0	18.0	33.0
2	30.191615000109884	31.0	29.0	33.0	25.0	33.0
3	31.32011467167419	33.0	31.0	33.0	27.0	34.0
4	30.7346449597941	33.0	31.0	33.0	27.0	33.0
5	31.382156052129705	33.0	31.0	33.0	29.0	33.0
6	34.67495415330743	37.0	34.0	38.0	28.0	38.0
7	35.91505114561789	38.0	36.0	38.0	31.0	38.0
8	35.76368893110664	38.0	36.0	38.0	31.0	38.0
9	36.35573175228379	38.0	37.0	38.0	33.0	38.0
10-14	36.65869412014642	38.0	37.8	38.0	34.0	38.0
15-19	36.68413462688973	38.0	38.0	38.0	34.0	38.0
20-24	36.70519573058017	38.0	38.0	38.0	34.0	38.0
25-29	36.65232896314439	38.0	38.0	38.0	34.0	38.0
30-34	36.625554006304995	38.0	38.0	38.0	34.0	38.0
35-39	36.59318592390548	38.0	38.0	38.0	34.0	38.0
40-44	36.55362146138011	38.0	38.0	38.0	34.0	38.0
45-49	36.530467843825775	38.0	38.0	38.0	34.0	38.0
50-54	36.47528166107878	38.0	38.0	38.0	34.0	38.0
55-59	36.38459170193179	38.0	37.0	38.0	33.8	38.0
60-64	36.32607945457698	38.0	37.0	38.0	33.0	38.0
65-69	36.23547838062889	38.0	37.0	38.0	33.0	38.0
70-74	36.131021764664226	38.0	37.0	38.0	33.0	38.0
75-79	35.986214421770995	38.0	37.0	38.0	31.8	38.0
80-84	35.833300150498424	38.0	37.0	38.0	31.0	38.0
85-89	35.671012211727444	38.0	36.0	38.0	30.6	38.0
90-94	35.5434343785915	38.0	36.0	38.0	29.0	38.0
95-99	35.39428098243236	38.0	36.0	38.0	29.0	38.0
100-104	35.23774122231158	38.0	35.2	38.0	28.0	38.0
105-109	35.0249198341185	38.0	35.0	38.0	27.6	38.0
110-114	34.836006238150034	38.0	34.8	38.0	27.0	38.0
115-119	34.6691246184116	38.0	34.0	38.0	26.2	38.0
120-124	34.79059969958469	38.0	34.0	38.0	28.0	38.0
125-129	33.77454751673274	34.2	33.2	35.6	31.0	36.2
130-134	35.43645889293631	36.2	36.0	37.2	33.2	38.0
135-139	36.617320348416385	38.0	38.0	38.0	34.0	38.0
140-144	36.56661592387403	38.0	38.0	38.0	34.0	38.0
145-149	36.54610535971566	38.0	38.0	38.0	34.0	38.0
150-154	36.53152159785803	38.0	38.0	38.0	34.0	38.0
155-159	36.5198204389413	38.0	38.0	38.0	34.0	38.0
160-164	36.50139816332485	38.0	38.0	38.0	34.0	38.0
165-169	36.44497003365889	38.0	38.0	38.0	34.0	38.0
170-174	36.407694110025645	38.0	38.0	38.0	34.0	38.0
175-179	36.34466894879854	38.0	38.0	38.0	33.2	38.0
180-184	36.265396166147795	38.0	37.6	38.0	33.0	38.0
185-189	36.151578293713825	38.0	37.0	38.0	33.0	38.0
190-194	36.03873571902013	38.0	37.0	38.0	32.4	38.0
195-199	35.911982898170905	38.0	37.0	38.0	31.0	38.0
200-204	35.73784377849237	38.0	37.0	38.0	30.2	38.0
205-209	35.56972040444646	38.0	37.0	38.0	29.0	38.0
210-214	35.387426788785625	38.0	36.2	38.0	28.4	38.0
215-219	35.12681297483539	38.0	36.0	38.0	27.0	38.0
220-224	34.94658567467407	38.0	35.0	38.0	25.8	38.0
225-229	34.738248942516186	38.0	35.0	38.0	24.8	38.0
230-234	34.51042077544842	38.0	34.6	38.0	23.6	38.0
235-239	34.30465737878789	38.0	34.0	38.0	23.0	38.0
240-244	34.15953349169567	38.0	33.8	38.0	23.0	38.0
245-249	34.40980672568294	38.0	33.4	38.0	24.6	38.0
250-252	35.67809904478903	37.666666666666664	34.333333333333336	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.0
17	19.0
18	37.0
19	33.0
20	124.0
21	388.0
22	1169.0
23	2680.0
24	5048.0
25	8688.0
26	13058.0
27	18619.0
28	25500.0
29	33725.0
30	43527.0
31	56753.0
32	73428.0
33	98750.0
34	139768.0
35	219414.0
36	419431.0
37	887421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.736011072618375	19.380111204968024	13.176728545458365	21.707149176955234
2	15.403312888049742	21.32743107836132	40.745241087157076	22.52401494643186
3	17.65368645297029	30.0136551574592	29.67554837959775	22.657110009972758
4	13.26395709274221	21.366013228305235	37.406859515554714	27.963170163397848
5	15.970445053077395	35.338712702239135	34.84241854758993	13.84842369709354
6	28.448098192107945	37.6413499265962	20.4559728615955	13.454579019700349
7	23.881132046906036	31.64555478337142	26.36122687090096	18.112086298821588
8	23.33717363349862	35.567495920800276	22.324613947273445	18.77071649842766
9	23.140904735439165	17.8139385870749	21.610953798187325	37.4342028792986
10-14	23.68569110004716	23.767485601471872	29.037018702751322	23.50980459572965
15-19	23.0929892895247	28.401342420985664	26.19945983173732	22.306208457752312
20-24	22.849115825052504	27.26610304880705	27.071776201109877	22.813004925030565
25-29	23.08110446051969	27.175623475851868	26.78541811108487	22.957853952543573
30-34	22.91520758987571	27.021418417021582	27.099688424224418	22.96368556887829
35-39	22.71262756002829	26.669783273102926	27.431961554386948	23.18562761248183
40-44	22.341466826507535	26.19330676786448	27.962132484530393	23.503093921097587
45-49	21.642564919987645	26.154909686094786	28.52630783095611	23.67621756296146
50-54	21.094650688920876	26.3179095790189	28.3211479628042	24.266291769256025
55-59	20.827059671473865	26.027761338328236	28.029625586557877	25.11555340364002
60-64	21.099087928812015	25.994522122539305	27.193771148731866	25.712618799916818
65-69	21.24223328263291	26.049109994651253	26.36449644634709	26.344160276368743
70-74	21.52481803286983	25.990692566145313	25.842272557936546	26.642216843048306
75-79	21.314478590959872	25.702598747501654	25.920868766640577	27.062053894897897
80-84	21.042443454198224	25.36646723795054	26.254494667547227	27.336594640304007
85-89	21.00218047520332	24.717672320741826	26.738358018750795	27.541789185304054
90-94	21.084346059020184	24.04504596560995	27.03843261499999	27.832175360369877
95-99	20.851161311719796	23.736741664741366	27.52752531752843	27.884571706010412
100-104	20.740583925247595	24.16033037456799	27.618401821233572	27.480683878950842
105-109	20.370231775416773	25.683092730684553	27.150431158044647	26.79624433585403
110-114	20.047517298829884	27.11157916298721	26.599699545095795	26.241203993087115
115-119	19.96380832471103	27.573680484544923	26.50344597191386	25.95906521883019
120-124	20.103404386352405	27.75084112902816	26.418181298862077	25.727573185757365
125-129	22.199644394305405	25.746107343870705	28.110860688403577	23.943387573420313
130-134	21.627908438547706	30.90615446143257	27.574370304835554	19.89156679518417
135-139	23.710207807355726	23.6958587225052	28.148907161519233	24.44502630861984
140-144	22.663348504636936	27.206096833001013	26.703638657537038	23.426916004825014
145-149	22.506120170427817	27.033180231677644	27.40614576484992	23.05455383304462
150-154	22.565333659140624	26.836967760792497	27.37196677079929	23.225731809267593
155-159	22.39080471510663	26.615891234451343	27.54483060849492	23.448473441947108
160-164	22.163094620510076	26.594328713366057	27.718134001771404	23.52444266435246
165-169	22.089798129621414	26.39396909076516	27.566574952939995	23.94965782667343
170-174	22.003493023284136	26.43012706063721	27.57627164257151	23.99010827350714
175-179	22.03369287261623	25.956447667414622	27.522793494013655	24.487065965955495
180-184	22.042020302429485	25.676350650824048	27.489797953058552	24.791831093687918
185-189	21.61911028297676	25.07363619788883	28.357244795956216	24.95000872317819
190-194	21.531137945290972	25.364981002280985	27.88983248680692	25.214048565621123
195-199	21.34415441017128	25.123298772756698	28.365710307367575	25.166836509704442
200-204	21.86042866312045	24.778804051587	27.75783347105378	25.60293381423877
205-209	22.06655753314665	24.327409223528264	27.97357994831056	25.632453295014525
210-214	22.112834765337013	24.54245994776046	27.27147223477933	26.073233052123197
215-219	21.665227307692916	24.726848922027187	27.304312604978044	26.303611165301856
220-224	21.83762428718597	24.81913971773622	27.935454682250686	25.407781312827122
225-229	21.54445239389748	25.348658776750533	27.580032613588646	25.526856215763345
230-234	21.846227255036812	25.622888725666904	27.44372634972894	25.087157669567343
235-239	22.07151374262794	25.26741676690908	27.4192183498965	25.241851140566478
240-244	22.058420662453052	25.13399918228567	27.533374951612764	25.274205203648513
245-249	21.623526038752438	25.916709911689175	27.334913365902114	25.124850683656273
250-252	20.638916086162073	26.549760257697603	27.550468514548744	25.26085514159158
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.5
13	1.5
14	0.6666666666666666
15	0.0
16	0.3333333333333333
17	1.3333333333333333
18	4.166666666666667
19	11.500000000000002
20	19.0
21	23.66666666666665
22	58.6666666666668
23	97.9999999999997
24	153.49999999999994
25	267.00000000000244
26	449.49999999999216
27	673.5000000000056
28	1029.6666666667124
29	1581.4999999999234
30	2406.6666666666315
31	3438.3333333337637
32	4880.000000000076
33	6810.833333331671
34	9241.499999998796
35	12610.500000004899
36	16740.666666677716
37	21796.16666665949
38	27794.333333304457
39	34617.333333297516
40	41899.00000001684
41	49813.33333340729
42	57326.000000128224
43	63234.833333504226
44	67397.83333350345
45	69332.83333347634
46	68847.6666668171
47	65329.33333351789
48	59821.500000144086
49	52962.1666667608
50	45827.500000042295
51	38700.166666657875
52	32496.833333288738
53	27143.666666641224
54	22964.999999989566
55	19548.333333335042
56	16714.16666667842
57	14514.166666674664
58	12759.500000004862
59	11168.66666666871
60	9638.49999999932
61	8364.833333330369
62	7090.833333331499
63	6033.999999999103
64	4987.333333333364
65	4055.333333334012
66	3212.8333333336336
67	2573.166666666683
68	2051.4999999998286
69	1530.1666666666106
70	1109.1666666667033
71	818.3333333333527
72	568.833333333325
73	424.16666666666134
74	279.1666666666691
75	173.50000000000023
76	108.49999999999963
77	68.83333333333348
78	44.66666666666669
79	27.833333333333304
80	12.66666666666667
81	8.666666666666664
82	4.0
83	1.5
84	1.5
85	0.3333333333333333
86	0.3333333333333333
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03584710769027904
2	2.9302812825841173E-4
3	3.418661496348137E-4
4	2.9302812825841173E-4
5	4.395421923876176E-4
6	3.418661496348137E-4
7	9.76760427528039E-5
8	9.76760427528039E-5
9	1.4651406412920587E-4
10-14	7.130351120954685E-4
15-19	3.907041710112156E-5
20-24	1.8558448123032746E-4
25-29	0.0023344574217920135
30-34	0.0028033024270054724
35-39	0.0028716756569324353
40-44	0.010519709804476982
45-49	0.006163358297701926
50-54	9.962956360785999E-4
55-59	1.7581687695504704E-4
60-64	1.7581687695504704E-4
65-69	8.790843847752352E-5
70-74	8.497815719493941E-4
75-79	8.497865521766502E-4
80-84	0.0025591758125456466
85-89	0.0020806001036783543
90-94	0.005440981807095607
95-99	0.0027352391111840507
100-104	0.0017584446127558158
105-109	0.0010845027969913353
110-114	3.225012966018038E-4
115-119	8.896788454901592E-4
120-124	8.510013106398347E-4
125-129	0.0014420216083504626
130-134	0.003123137043842755
135-139	0.0023177998187009123
140-144	0.0012571117660750712
145-149	0.001610674450283685
150-154	0.0017481717364629143
155-159	0.0020820977016489527
160-164	0.0023669250412517713
165-169	0.0021901598964044547
170-174	0.0031526968964792823
175-179	0.004409944513272755
180-184	0.005097694015385804
185-189	0.0036345254773359832
190-194	0.0026428689255892884
195-199	0.0017689963923284131
200-204	0.0013963605926193689
205-209	0.0020279993864809626
210-214	0.004322023938684097
215-219	0.011765276100898295
220-224	0.013469158321275944
225-229	0.015066747707964706
230-234	0.030717715266151017
235-239	0.0767325645861305
240-244	0.031106113830539548
245-249	9.331351509795178E-5
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	34.0
80-84	44.0
85-89	55.0
90-94	68.0
95-99	78.0
100-104	142.0
105-109	400.0
110-114	699.0
115-119	968.0
120-124	1573.0
125-129	7110.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	4.0
155-159	6.0
160-164	17.0
165-169	19.0
170-174	35.0
175-179	72.0
180-184	141.0
185-189	269.0
190-194	495.0
195-199	861.0
200-204	1815.0
205-209	3319.0
210-214	7229.0
215-219	11432.0
220-224	16716.0
225-229	26514.0
230-234	43046.0
235-239	54580.0
240-244	86818.0
245-249	256691.0
250-253	1526335.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.9759001469338
#Duplication Level	Percentage of total
1	87.38811669092237
2	9.306658925952632
3	2.0351252233816455
4	0.6644241875226043
5	0.21738668694725521
6	0.1135825999641136
7	0.062189299667964756
8	0.051881796101118954
9	0.019312204239784525
>10	0.13854049997186033
>50	0.0027818853286324255
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	9.767604275280392E-5	0.0	0.0
2	0.0	0.0	0.0	9.767604275280392E-5	0.0	0.0
3	0.0	0.0	0.0	9.767604275280392E-5	0.0	0.0
4	0.0	0.0	0.0	9.767604275280392E-5	0.0	0.0
5	4.883802137640196E-5	0.0	0.0	9.767604275280392E-5	0.0	0.0
6	4.883802137640196E-5	0.0	4.883802137640196E-5	9.767604275280392E-5	0.0	0.0
7	4.883802137640196E-5	0.0	4.883802137640196E-5	9.767604275280392E-5	0.0	0.0
8	4.883802137640196E-5	0.0	9.767604275280392E-5	1.4651406412920587E-4	0.0	0.0
9	4.883802137640196E-5	0.0	9.767604275280392E-5	1.9535208550560784E-4	0.0	0.0
10-14	1.269788555786451E-4	7.814083420224314E-5	1.3674645985392548E-4	2.2465489833144902E-4	5.8605625651682344E-5	0.0
15-19	2.2465489833144902E-4	1.4651406412920587E-4	1.6604927267976663E-4	9.083871976010764E-4	3.223309410842529E-4	0.0
20-24	3.5163375391009403E-4	1.4651406412920587E-4	1.9535208550560784E-4	0.005147527453072766	5.469858394157019E-4	0.0
25-29	4.004717752864961E-4	1.6604927267976663E-4	1.9535208550560784E-4	0.08604282606094497	6.641970907190666E-4	0.0
30-34	5.372182351404216E-4	1.9535208550560784E-4	1.9535208550560784E-4	0.7818283490062684	0.0015042110583931804	0.0
35-39	5.372182351404216E-4	2.441901068820098E-4	1.9535208550560784E-4	2.404207883921791	0.0016604927267976663	0.0
40-44	5.860562565168235E-4	2.539577111572902E-4	2.9302812825841173E-4	5.436257835450054	0.001670260331072947	0.0
45-49	5.860562565168235E-4	2.9302812825841173E-4	2.9302812825841173E-4	8.756540021537567	0.0019046828336796765	0.0
50-54	6.153590693426646E-4	2.9302812825841173E-4	2.9302812825841173E-4	12.146084289541093	0.002891210865482996	0.0
55-59	6.544294864437863E-4	3.320985453595333E-4	3.320985453595333E-4	15.443305161934669	0.003946112127213278	0.0
60-64	7.325703206460294E-4	3.614013581853744E-4	3.418661496348137E-4	18.7049426519534	0.005098689431696364	0.0
65-69	7.716407377471509E-4	3.907041710112157E-4	3.418661496348137E-4	21.84782560919327	0.006192661110527768	0.0
70-74	7.814083420224314E-4	3.907041710112157E-4	3.418661496348137E-4	24.962978337895617	0.007032675078201882	0.0
75-79	7.814083420224314E-4	3.907041710112157E-4	3.5163375391009403E-4	27.960109104139754	0.007950829880078239	0.0
80-84	8.107111548482724E-4	3.907041710112157E-4	4.49309796662898E-4	30.902218955501233	0.00875177343065123	0.0
85-89	8.790843847752352E-4	3.907041710112157E-4	4.883802137640196E-4	33.74268711677415	0.009816442296656793	0.0
90-94	9.47457614702198E-4	3.907041710112157E-4	4.883802137640196E-4	36.48830207292982	0.014641638808645307	0.0
95-99	0.001015830844629161	3.907041710112157E-4	4.883802137640196E-4	39.141876893999516	0.07017046911361433	0.0
100-104	0.0010744364702808431	3.907041710112157E-4	4.883802137640196E-4	41.69956314389879	0.20216010568547826	3.907041710112157E-5
105-109	0.0011037392831066842	3.907041710112157E-4	4.883802137640196E-4	44.16687951904316	0.4420329314778141	4.883802137640196E-5
110-114	0.0012111829301347684	3.907041710112157E-4	4.981478180393E-4	46.517805121643306	0.6546736765506682	4.883802137640196E-5
115-119	0.001220950534410049	4.395421923876176E-4	5.372182351404216E-4	47.87056947574826	0.8090799649343007	4.883802137640196E-5
120-124	0.0012600209515111704	4.395421923876176E-4	5.372182351404216E-4	47.90175743619923	0.8287519199447153	4.883802137640196E-5
125-129	0.0013088589728875726	4.590774009381784E-4	5.762886522415431E-4	47.90239233047713	0.8287812227575412	1.1721125130336471E-4
130-134	0.0013381617857134136	4.883802137640196E-4	6.348942778932255E-4	47.90368165424146	0.8289277368216705	1.4651406412920587E-4
135-139	0.0013674645985392547	5.079154223145805E-4	7.130351120954685E-4	47.90602587926753	0.8290937860943501	1.4651406412920587E-4
140-144	0.0013967674113650958	5.469858394157019E-4	7.325703206460294E-4	47.90952268159808	0.82925983536703	1.4651406412920587E-4
145-149	0.0014846758498426196	5.860562565168235E-4	7.325703206460294E-4	47.91444555415282	0.8294161170354345	1.4651406412920587E-4
150-154	0.0015139786626684607	5.860562565168235E-4	7.325703206460294E-4	47.923197327583466	0.8297091451636929	1.4651406412920587E-4
155-159	0.0015139786626684607	5.958238607921038E-4	8.009435505729922E-4	47.93411750916324	0.8298458916235468	1.9535208550560784E-4
160-164	0.001553049079769582	6.348942778932255E-4	8.790843847752352E-4	47.948397746613686	0.8298947296449232	1.9535208550560784E-4
165-169	0.0015628166840448627	7.032675078201883E-4	9.279224061516371E-4	47.964397082416596	0.8300412437090523	2.539577111572902E-4
170-174	0.0016018871011459841	7.911759462977117E-4	9.279224061516371E-4	47.98019129852973	0.8301779901689063	3.223309410842529E-4
175-179	0.0016116547054212643	8.302463633988331E-4	9.279224061516371E-4	47.99813438758342	0.8302854338159344	3.907041710112157E-4
180-184	0.0016604927267976663	8.302463633988331E-4	9.279224061516371E-4	48.02222129972626	0.8304417154843389	3.907041710112157E-4
185-189	0.0016604927267976663	8.302463633988331E-4	9.669928232527587E-4	48.04924826075596	0.8308324196553499	3.907041710112157E-4
190-194	0.0017093307481740685	8.693167804999548E-4	9.767604275280391E-4	48.074057975615176	0.8314380111204175	3.907041710112157E-4
195-199	0.0017581687695504705	8.790843847752352E-4	9.767604275280391E-4	48.09982491569336	0.8331766446814173	3.907041710112157E-4
200-204	0.0017874715823763116	9.181548018763567E-4	0.0010255984489044411	48.125796975461334	0.8412642210213495	3.907041710112157E-4
205-209	0.0018070067909268725	0.001045133657455002	0.0010255984489044411	48.14877038071679	0.8607505915505339	3.907041710112157E-4
210-214	0.0018070067909268725	0.0010744364702808431	0.001045133657455002	48.170815863566105	0.8875333624733528	3.907041710112157E-4
215-219	0.0018363096037527134	0.0010744364702808431	0.0010744364702808431	48.202101500059825	0.9210753155546657	4.004717752864961E-4
220-224	0.0018558448123032743	0.0010744364702808431	0.0010744364702808431	48.2324982845645	0.9678133020118823	4.395421923876176E-4
225-229	0.0018558448123032743	0.0010939716788314039	0.0010744364702808431	48.26280716063069	1.0134084787688913	4.395421923876176E-4
230-234	0.001875380020853835	0.001123274491657245	0.0010744364702808431	48.29151414959574	1.0476146289409232	4.395421923876176E-4
235-239	0.0019925912721571993	0.001123274491657245	0.0010744364702808431	48.33128783420469	1.0707540834690623	4.395421923876176E-4
240-241	0.00200235887643248	0.001123274491657245	0.0010744364702808431	48.41273988625625	1.0717015410837645	4.395421923876176E-4
>>END_MODULE
